FastQCFastQC Report
Thu 26 May 2016
SRR936935_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936935_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950807
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76840.8081555983496126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.6193685995159901No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.4056554064073992No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28270.29732637643601695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13740.14450882250551375No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12330.12967931451914005No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.10454277261315913No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9910.10422725116664056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99750.028.71951
GTACATG53500.025.6635381
GGTATCA69400.025.260351
GAGTACT60550.024.19910412-13
ATCAACG118450.024.139023
TCAACGC118300.024.0893334
CAACGCA118900.023.9677725
TATCAAC120550.023.7591692
TACATGG56900.023.6241022
AACGCAG120850.023.5810366
ATTCCGT1652.4308665E-623.0284296
ACATGGG54750.022.9889973
AGTACTT62950.022.86152312-13
GTACTTT65600.022.4448214-15
AGAGTAC92900.022.29097410-11
CATGGGG19850.022.0133234
TATTCCG1302.942609E-421.9212955
ACGCAGA131200.021.5759897
CGCAGAG130650.021.5214028
CCGTTGC1353.7973654E-421.1093929
Produced by FastQC (version 0.11.5)