##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936935_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 950807 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.24202072555208 33.0 31.0 34.0 31.0 34.0 2 32.23187671104651 33.0 31.0 34.0 30.0 34.0 3 32.40152417893432 34.0 31.0 34.0 31.0 34.0 4 36.13239700591182 37.0 35.0 37.0 35.0 37.0 5 36.080311777258686 37.0 35.0 37.0 35.0 37.0 6 36.13737383086157 37.0 35.0 37.0 35.0 37.0 7 36.08293376047926 37.0 35.0 37.0 35.0 37.0 8 36.06872057105175 37.0 35.0 37.0 35.0 37.0 9 37.76151416638708 39.0 37.0 39.0 35.0 39.0 10-11 37.69602821603122 39.0 37.0 39.0 35.0 39.0 12-13 37.60882965733319 39.0 37.0 39.0 35.0 39.0 14-15 38.798641574998925 40.0 38.0 41.0 35.0 41.0 16-17 38.724532423509714 40.0 38.0 41.0 35.0 41.0 18-19 38.82486298481185 40.0 38.0 41.0 35.0 41.0 20-21 38.91322949873108 40.0 38.0 41.0 35.0 41.0 22-23 38.84268468784937 40.0 38.0 41.0 35.0 41.0 24-25 38.732648160983246 40.0 38.0 41.0 35.0 41.0 26-27 38.56565002150805 40.0 38.0 41.0 34.0 41.0 28-29 38.391350715760396 40.0 38.0 41.0 34.0 41.0 30-31 38.2070357075621 40.0 37.5 41.0 34.0 41.0 32-33 38.139009283692694 40.0 37.0 41.0 34.0 41.0 34-35 38.02311773051734 40.0 37.0 41.0 34.0 41.0 36-37 37.83307127524303 40.0 37.0 41.0 33.0 41.0 38-39 37.64828614008942 40.0 37.0 41.0 33.0 41.0 40-41 37.447963151302 40.0 37.0 41.0 33.0 41.0 42-43 37.16672784276935 40.0 36.0 41.0 32.5 41.0 44-45 36.7229038069766 40.0 36.0 41.0 31.0 41.0 46-47 36.30890864286864 39.0 36.0 41.0 30.5 41.0 48-49 35.85506680114892 39.0 35.0 41.0 28.5 41.0 50-51 35.549079361005965 39.0 35.0 41.0 27.5 41.0 52-53 35.3007250682841 39.0 35.0 40.0 27.0 41.0 54-55 35.05015108218598 38.5 35.0 40.0 26.5 41.0 56-57 34.77023623090701 38.0 34.5 40.0 26.0 41.0 58-59 34.4355026835099 38.0 34.0 40.0 25.0 41.0 60-61 34.08714281657582 37.5 34.0 40.0 24.5 41.0 62-63 33.72966122462287 37.0 33.5 40.0 23.0 41.0 64-65 33.3325990448114 36.5 33.0 39.0 22.0 41.0 66-67 32.944918895212176 36.0 33.0 39.0 21.5 40.0 68-69 32.5015891763523 35.5 32.0 38.5 20.0 40.0 70-71 32.021829877146466 35.0 31.5 38.0 18.5 40.0 72-73 31.696857511566492 35.0 31.0 37.0 18.0 39.5 74-75 31.26187386083611 35.0 31.0 37.0 14.5 39.0 76-77 29.90340205741018 33.5 29.5 35.5 12.0 37.5 78-79 30.598066169054288 34.0 31.0 36.0 9.5 38.0 80-81 30.419226509691242 34.0 31.0 35.5 7.0 37.0 82-83 30.098612021156768 34.0 30.5 35.0 4.5 37.0 84-85 29.728959715273447 34.0 30.0 35.0 2.0 36.5 86-87 29.395917362829678 34.0 30.0 35.0 2.0 36.0 88-89 29.056935845024277 34.0 29.5 35.0 2.0 36.0 90-91 28.67191238600473 34.0 29.0 35.0 2.0 35.0 92-93 28.24738669361921 34.0 29.0 35.0 2.0 35.0 94-95 27.801678468921665 33.5 28.0 35.0 2.0 35.0 96-97 27.199941207837135 33.0 27.0 35.0 2.0 35.0 98-99 26.53412942900084 33.0 25.5 35.0 2.0 35.0 100-101 23.637469013164605 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3178036104372737 1101 2 0.18255797038639798 1101 3 0.19621523232378735 1101 4 0.18563002674846985 1101 5 0.2714108927352825 1101 6 0.2106879074572987 1101 7 0.25878821438868727 1101 8 0.3156553324164051 1101 9 0.3540030616133265 1101 10-11 0.38444056208851407 1101 12-13 0.3092793287796667 1101 14-15 0.41774329578798586 1101 16-17 0.49690979105714206 1101 18-19 0.38530398801944443 1101 20-21 0.3980271473671593 1101 22-23 0.3423809618365752 1101 24-25 0.3700048397791633 1101 26-27 0.3804079114337853 1101 28-29 0.3960883146833183 1101 30-31 0.4633748570143368 1101 32-33 0.5572015105874684 1101 34-35 0.5988430734724872 1101 36-37 0.5072984884730971 1101 38-39 0.6141507058609363 1101 40-41 0.4597543350543276 1101 42-43 0.6184327857329137 1101 44-45 0.567533692520577 1101 46-47 0.5369654243156177 1101 48-49 0.4965812893588719 1101 50-51 0.5440778652601495 1101 52-53 0.42195229741475515 1101 54-55 0.7138377508699136 1101 56-57 0.7889222563177398 1101 58-59 0.7700835894998264 1101 60-61 0.6820650204903203 1101 62-63 0.6816636203101822 1101 64-65 0.6133164472491472 1101 66-67 0.6865186866810333 1101 68-69 0.5492360960294818 1101 70-71 0.49792814593331514 1101 72-73 0.6372620491440557 1101 74-75 0.5734176267987294 1101 76-77 0.2896952233896606 1101 78-79 0.2711735056165274 1101 80-81 0.6644672026324514 1101 82-83 0.649030628325157 1101 84-85 0.543191156077441 1101 86-87 0.3324863247265206 1101 88-89 0.4766533527747647 1101 90-91 0.48311651620441864 1101 92-93 0.652068900143373 1101 94-95 0.6149224621306573 1101 96-97 0.8079549597307896 1101 98-99 0.7597357084767253 1101 100-101 0.5858076803107366 1102 1 0.15219026052066198 1102 2 0.1128519810541917 1102 3 0.14004431639187231 1102 4 0.18910432600712568 1102 5 0.23172904765514346 1102 6 0.13096771420693187 1102 7 0.18629282925694923 1102 8 0.16335705174087423 1102 9 0.39497442012294925 1102 10-11 0.3008124847401916 1102 12-13 0.2747776181268904 1102 14-15 0.40161956031006696 1102 16-17 0.40130433399046694 1102 18-19 0.2950268068668933 1102 20-21 0.2569916343600198 1102 22-23 0.2234731299293884 1102 24-25 0.21333658597786354 1102 26-27 0.20858948070578975 1102 28-29 0.13395737366771954 1102 30-31 0.07570745512695254 1102 32-33 0.0942236338524225 1102 34-35 0.15993798760261058 1102 36-37 0.16536465191370553 1102 38-39 0.35193906988914136 1102 40-41 0.2952165164196572 1102 42-43 0.33276260408494807 1102 44-45 0.3268041779991009 1102 46-47 0.46271402058469846 1102 48-49 0.330644356682555 1102 50-51 0.30456800883293766 1102 52-53 0.6010208287103538 1102 54-55 0.5976024877361112 1102 56-57 0.5229035045763695 1102 58-59 0.5152810352033654 1102 60-61 0.5865884344731072 1102 62-63 0.49621055225583177 1102 64-65 0.518664876828943 1102 66-67 0.46388210544931496 1102 68-69 0.24353670361031732 1102 70-71 0.21027872978741158 1102 72-73 0.4344170099568174 1102 74-75 0.42661985157071314 1102 76-77 0.3935612745799695 1102 78-79 0.6259751766846158 1102 80-81 0.5775278218076636 1102 82-83 0.6962030529717218 1102 84-85 0.573422858997958 1102 86-87 0.5776400104786816 1102 88-89 0.6589553028627257 1102 90-91 0.6749348505802288 1102 92-93 0.6004128870844383 1102 94-95 0.5895982972431604 1102 96-97 0.5933260481331182 1102 98-99 0.7969959019358548 1102 100-101 0.9436825846611185 1103 1 -0.022944865125843705 1103 2 -0.11341545522091678 1103 3 -0.056179506255617184 1103 4 0.07724566416194278 1103 5 0.08855012007482799 1103 6 0.16027964659543414 1103 7 0.20754749103191017 1103 8 0.1326423978492457 1103 9 0.11898537379803287 1103 10-11 0.2896078677278524 1103 12-13 0.20424937140110444 1103 14-15 0.10204356839127371 1103 16-17 0.22050406461686833 1103 18-19 0.23321051391353365 1103 20-21 0.17068316967780106 1103 22-23 0.18651604618579398 1103 24-25 0.30668857970950825 1103 26-27 0.23575145115447782 1103 28-29 0.22838335098479945 1103 30-31 0.28394497305992417 1103 32-33 0.3238394892332721 1103 34-35 0.40008741973179696 1103 36-37 0.43590718118494465 1103 38-39 0.466380159575543 1103 40-41 0.5100559215636054 1103 42-43 0.5330880705816057 1103 44-45 0.544724703213781 1103 46-47 0.4047963040046554 1103 48-49 0.34531947265842433 1103 50-51 0.18151669858892205 1103 52-53 0.3524219543983449 1103 54-55 0.4266196730692968 1103 56-57 0.43816651884774416 1103 58-59 0.37684247647976576 1103 60-61 0.2600878496068617 1103 62-63 0.37958399374286955 1103 64-65 0.23105071620377515 1103 66-67 0.23101517568592556 1103 68-69 0.20877785521435044 1103 70-71 0.1804250181607827 1103 72-73 0.3635388807628175 1103 74-75 0.4140775425219658 1103 76-77 0.1786967353476534 1103 78-79 0.09828698619535459 1103 80-81 0.19459123471365558 1103 82-83 0.11192526304801831 1103 84-85 0.07554609103426557 1103 86-87 -0.1527801357299019 1103 88-89 0.17915991827925737 1103 90-91 0.20983373175424092 1103 92-93 0.14559799366095305 1103 94-95 0.14692436536212483 1103 96-97 0.5109341127444793 1103 98-99 0.5573547369269782 1103 100-101 0.7279136172013807 1104 1 0.23128101158985004 1104 2 0.03953535951997367 1104 3 0.13456930511978982 1104 4 0.15996544248589117 1104 5 0.19211661315895867 1104 6 0.20738757742429925 1104 7 0.09526028889583671 1104 8 0.17941767304215972 1104 9 0.27162034262926227 1104 10-11 0.19054416948189612 1104 12-13 0.26552480495933395 1104 14-15 0.2493427762108098 1104 16-17 0.34976555141061993 1104 18-19 0.5054891519364304 1104 20-21 0.22691745479552594 1104 22-23 0.2147417884236873 1104 24-25 0.08340852436126767 1104 26-27 0.07633729665717226 1104 28-29 0.22693682980189323 1104 30-31 0.3033863062304718 1104 32-33 0.17637262651211216 1104 34-35 0.2092545597249682 1104 36-37 0.20322164161927248 1104 38-39 0.0947564815720412 1104 40-41 0.1318304447424481 1104 42-43 0.2251219412953489 1104 44-45 0.41973731050848784 1104 46-47 0.17085091969008204 1104 48-49 0.35578308490934774 1104 50-51 0.32637805373596507 1104 52-53 0.2054753084695733 1104 54-55 0.1040427951132159 1104 56-57 0.13172166979024524 1104 58-59 0.33965493832610605 1104 60-61 0.35594487138342146 1104 62-63 0.4699216746022614 1104 64-65 0.4437260460725341 1104 66-67 0.42811717887447287 1104 68-69 0.3636675391738038 1104 70-71 0.5123197928131304 1104 72-73 0.3325072413190604 1104 74-75 0.41575497626067914 1104 76-77 0.35662061029036707 1104 78-79 0.2495842547033078 1104 80-81 0.5181669643000149 1104 82-83 0.47365685949426606 1104 84-85 0.4809781098470012 1104 86-87 0.31599491297406956 1104 88-89 0.575461601552167 1104 90-91 0.7924305336595765 1104 92-93 0.6907077788680382 1104 94-95 0.650910482297661 1104 96-97 0.6847997658731231 1104 98-99 0.5938734249189928 1104 100-101 0.5365715994326195 1105 1 0.10376165968349227 1105 2 -0.09889234063660268 1105 3 -0.002771164291580419 1105 4 0.06639452020186809 1105 5 0.11032302281468276 1105 6 0.04611902141889601 1105 7 0.13246039942094967 1105 8 0.08168203630136617 1105 9 0.1082283829308821 1105 10-11 0.09667641117950154 1105 12-13 0.13151610711337014 1105 14-15 0.22697295357157543 1105 16-17 0.20210890408585414 1105 18-19 0.1485315759604262 1105 20-21 0.14766273845663136 1105 22-23 0.22607211435263963 1105 24-25 0.13603609877551293 1105 26-27 0.3522541345176293 1105 28-29 0.3895499078061917 1105 30-31 0.3438145710262219 1105 32-33 0.32398071887266866 1105 34-35 0.25163153045583186 1105 36-37 0.15981500530696735 1105 38-39 0.3125340057293897 1105 40-41 0.13085433979164662 1105 42-43 0.1454127104460312 1105 44-45 0.3374231308571254 1105 46-47 0.15417253976399792 1105 48-49 0.046771784116849346 1105 50-51 0.03196627960765852 1105 52-53 0.12255894714855486 1105 54-55 -0.03840245290754751 1105 56-57 -0.09665835639940923 1105 58-59 0.03131239456657653 1105 60-61 0.24442312877078365 1105 62-63 0.2807350153039323 1105 64-65 0.00803734646457599 1105 66-67 0.13361380105077103 1105 68-69 0.17304675644775358 1105 70-71 -0.046660472847595 1105 72-73 0.18385947204424014 1105 74-75 0.22943155815137928 1105 76-77 0.12143988571014219 1105 78-79 0.10031127769332926 1105 80-81 0.2263561344406142 1105 82-83 0.1946505243228529 1105 84-85 0.17538367694896095 1105 86-87 0.15470032750331697 1105 88-89 0.36806633010488454 1105 90-91 0.570769613497955 1105 92-93 0.683494613564914 1105 94-95 0.8004762907928722 1105 96-97 1.0040256295508065 1105 98-99 1.2139313304680748 1105 100-101 1.176965024790121 1106 1 0.4093372082414959 1106 2 0.38209681315956345 1106 3 0.37476419833149777 1106 4 0.1578150230258757 1106 5 0.23418717036304315 1106 6 0.2901036268070101 1106 7 0.21724072630654234 1106 8 0.12326498227027827 1106 9 0.39380593078913506 1106 10-11 0.3622022271439107 1106 12-13 0.42744003373905315 1106 14-15 0.5732355116762449 1106 16-17 0.601142992155026 1106 18-19 0.49424432649773564 1106 20-21 0.40769922568610184 1106 22-23 0.48935621491744286 1106 24-25 0.5686046997651601 1106 26-27 0.4448704688006373 1106 28-29 0.47479007374811033 1106 30-31 0.578698611612019 1106 32-33 0.6512086890830986 1106 34-35 0.5210397375833438 1106 36-37 0.5413302249800793 1106 38-39 0.7480040911994763 1106 40-41 0.4632130142555795 1106 42-43 0.5392498674410788 1106 44-45 0.5447180776257525 1106 46-47 0.38190158459831025 1106 48-49 0.46356965486209134 1106 50-51 0.4141587622387348 1106 52-53 0.4949173716999624 1106 54-55 0.45114870418747444 1106 56-57 0.4811831490736793 1106 58-59 0.31227280842172433 1106 60-61 0.06741133289933288 1106 62-63 0.2578067901827268 1106 64-65 0.412134662403993 1106 66-67 0.5153776836918453 1106 68-69 0.6062601317663976 1106 70-71 0.3869964972128841 1106 72-73 0.31191340316834726 1106 74-75 0.33837467805940946 1106 76-77 0.2818449939222667 1106 78-79 0.24690996815166955 1106 80-81 0.2975749578524187 1106 82-83 0.48273844355112416 1106 84-85 0.38525147322026854 1106 86-87 0.20433795433392632 1106 88-89 0.4127019576352531 1106 90-91 0.4859517997327707 1106 92-93 0.536738478212289 1106 94-95 0.46620703503239014 1106 96-97 0.8264490313601733 1106 98-99 0.7248750001836726 1106 100-101 1.020251124655168 1107 1 0.10266150126068396 1107 2 -0.025533777003445834 1107 3 -0.03441172053167918 1107 4 -0.004763726585672146 1107 5 0.09927743224964303 1107 6 0.05139978184840288 1107 7 0.07164364180785299 1107 8 -0.0226569885182073 1107 9 -0.009400555636268848 1107 10-11 0.018543159991324387 1107 12-13 0.2182966035048466 1107 14-15 0.2691970338391343 1107 16-17 0.2801101362632821 1107 18-19 0.27335555061277006 1107 20-21 0.2698683360626859 1107 22-23 0.32185190664272767 1107 24-25 0.14765377172041383 1107 26-27 0.29999660943401807 1107 28-29 0.10892149731508027 1107 30-31 0.33045864777326983 1107 32-33 0.13083690616363697 1107 34-35 0.07939072778024325 1107 36-37 0.20797994105771522 1107 38-39 0.07674805255213357 1107 40-41 0.16403511782368696 1107 42-43 0.08107544587993232 1107 44-45 0.16276197965134998 1107 46-47 0.16990480521022988 1107 48-49 0.07891841323144178 1107 50-51 0.054519527275317614 1107 52-53 -0.05194818187802497 1107 54-55 0.12631326615600358 1107 56-57 0.11890668496655366 1107 58-59 0.16564173799202564 1107 60-61 0.22032965931118298 1107 62-63 0.01262640766443468 1107 64-65 0.05928272579919991 1107 66-67 0.04920964685256024 1107 68-69 0.07266830195030849 1107 70-71 0.18263454563506798 1107 72-73 0.33117068485888623 1107 74-75 0.27997283609540347 1107 76-77 0.25207269683494715 1107 78-79 0.2934110840654469 1107 80-81 0.21181204009102572 1107 82-83 -0.17621288000736968 1107 84-85 -0.054791468271950805 1107 86-87 0.004374680610165882 1107 88-89 0.16407495534692274 1107 90-91 0.25013944275336897 1107 92-93 0.13328338314773802 1107 94-95 0.1402712212628643 1107 96-97 0.5291752510963086 1107 98-99 0.6539946995932304 1107 100-101 0.7900833138237431 1108 1 0.06827984340539928 1108 2 -0.06230239927125325 1108 3 0.08329176325274545 1108 4 0.12402138743626523 1108 5 0.1256622661484883 1108 6 0.021683554435789176 1108 7 0.2046848514661832 1108 8 0.06694048248313322 1108 9 0.1402113160594567 1108 10-11 0.059733091341442446 1108 12-13 0.021486702534282642 1108 14-15 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-1.0661491124643092 2316 70-71 -0.9926119811181664 2316 72-73 -0.7558147247190803 2316 74-75 -0.908897917650215 2316 76-77 -1.0977590315038164 2316 78-79 -0.8111948754957048 2316 80-81 -0.9468115988274945 2316 82-83 -0.7748431837629965 2316 84-85 -0.7543543333678038 2316 86-87 -0.6049018234661183 2316 88-89 -0.977784967522549 2316 90-91 -1.1117488314665067 2316 92-93 -1.2737369807152277 2316 94-95 -0.8744574497108424 2316 96-97 -0.7695745245070889 2316 98-99 -0.8136220330683948 2316 100-101 -0.7424480036886862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 7.0 5 21.0 6 44.0 7 98.0 8 241.0 9 490.0 10 896.0 11 1530.0 12 2579.0 13 3702.0 14 4704.0 15 6717.0 16 9463.0 17 13750.0 18 11923.0 19 6238.0 20 4828.0 21 4852.0 22 4970.0 23 5093.0 24 5893.0 25 6814.0 26 8260.0 27 10174.0 28 12718.0 29 16228.0 30 20430.0 31 25867.0 32 33726.0 33 44874.0 34 63119.0 35 91692.0 36 143207.0 37 214553.0 38 158381.0 39 12721.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02275902113909 19.676938871684392 15.325522586508686 20.974779520667834 2 15.660569853907885 21.656867591531075 39.55246799962136 23.13009455493968 3 19.151118029407435 26.656219104314356 30.73644796903595 23.456214897242265 4 12.87298657453421 17.74044099473604 37.75947749538549 31.627094935344264 5 13.218249360527734 38.35792356623586 33.520862782714055 14.902964290522347 6 30.438344811338613 37.343658629959485 17.379817501819513 14.838179056882387 7 27.20720398566691 34.50153396009916 21.148140474354943 17.143121579878986 8 26.847194015189203 33.537615940984864 20.000273451920318 19.61491659190561 9 27.493592285290287 16.411322171586875 20.78686841809116 35.30821712503168 10-11 24.70062241929479 27.21328010130353 26.993834704450748 21.09226277495094 12-13 26.525911587131056 24.950449647428666 27.841434664790356 20.682204100649926 14-15 23.49535710191448 25.386329717808138 27.434589774791306 23.683723405486077 16-17 21.34213357705612 28.890353142120322 27.67848785295018 22.089025427873374 18-19 21.174381341323738 26.577265417692548 31.56765778964606 20.68069545133765 20-21 22.42033587407299 25.3850288133971 31.596027653288427 20.59860765924148 22-23 22.102224741719404 25.574958955918497 31.502292263308956 20.82052403905314 24-25 21.902429968862194 25.712222732333203 31.69882206435076 20.686525234453843 26-27 21.65142323452448 25.830097434559818 31.74558683707898 20.772892493836718 28-29 21.241562826213283 26.12004385884184 31.80639003342002 20.83200328152486 30-31 21.453080639511583 25.811565191871356 31.64738713929121 21.08796702932585 32-33 21.416987186566246 26.0584518915144 31.4589098221601 21.065651099759254 34-35 21.918022174390224 25.793886397663396 31.397644989937223 20.890446438009153 36-37 21.930185301659492 25.700744866813775 31.172865475917515 21.19620435560922 38-39 21.65021534837003 26.022392023433266 31.3517777415504 20.975614886646298 40-41 21.77583570161913 25.97432785209275 31.245017133697534 21.004819312590588 42-43 21.54159665743728 26.38889108010108 30.528757030504305 21.54075523195734 44-45 21.991779579997118 26.170720959020954 30.33441207063924 21.50308739034269 46-47 22.394029492841344 26.576161092629736 29.70445106104604 21.325358353482883 48-49 22.37095007438438 27.014175889996313 29.067805211271086 21.547068824348223 50-51 22.037314676871347 27.19089235390414 29.127197939010657 21.644595030213857 52-53 22.488403119706728 27.456458782628633 28.43947877275268 21.615659324911952 54-55 22.01557489211274 27.28903478371536 28.525517979807752 22.169872344364148 56-57 22.299478598341928 27.464115838988647 28.37602972226093 21.860375840408498 58-59 22.82327463803632 26.910051262849894 28.407864650930915 21.85880944818287 60-61 22.317464307444258 27.122030177951363 29.117153198880725 21.443352315723658 62-63 22.179527496116457 27.03808449033295 29.26440381696811 21.517984196582482 64-65 22.204722421906922 27.251764163926445 28.772055664439954 21.77145774972668 66-67 22.258735084373107 27.320385378097434 28.43165249711692 21.989227040412533 68-69 22.128477245370114 27.231355696453484 28.699793280756914 21.940373777419488 70-71 22.327799592660313 27.29959450086164 28.58098439898465 21.7916215074934 72-73 22.28648926648626 27.188062351244785 28.553165889607463 21.972282492661495 74-75 22.150908232304694 27.95626433656604 28.00017458926823 21.892652841861036 76-77 22.303697685364128 27.495234263867964 28.244447424398995 21.956620626368917 78-79 22.18469842364988 27.525494213318414 28.065510872903353 22.224296490128353 80-81 22.21307147470081 27.864236564704196 27.911568017401144 22.01112394319384 82-83 22.250853504363757 27.55309817351118 28.00482968827896 22.191218633846102 84-85 22.006020459464974 27.737605152941125 27.855458486807365 22.40091590078653 86-87 22.085832143090034 27.65012316125816 27.883614188681786 22.380430506970022 88-89 22.122031370540384 27.611060831629057 27.37962329478495 22.887284503045606 90-91 21.958836942208908 27.420477827186257 27.746096987391724 22.87458824321311 92-93 22.195525588013965 27.438705982763057 27.743920555864943 22.62184787335804 94-95 21.99731279629576 27.252768056457572 27.74882270503075 23.001096442215918 96-97 21.95196600703629 27.378376814947647 27.802920744437493 22.86673643357857 98-99 21.80162404268936 27.764808832368942 27.322590874501916 23.110976250439773 100-101 21.371957151646782 28.116576128397814 27.231211775408998 23.280254944546407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 634.0 1 438.0 2 284.5 3 340.0 4 313.5 5 330.0 6 535.5 7 1053.5 8 1798.0 9 2230.0 10 2096.0 11 1874.5 12 1908.5 13 2067.5 14 2202.0 15 2282.5 16 2409.0 17 2507.0 18 2583.0 19 2658.0 20 2795.0 21 3071.5 22 3442.0 23 3819.5 24 4263.5 25 4884.5 26 5766.5 27 6928.5 28 8072.5 29 9311.0 30 10957.0 31 12727.5 32 14508.5 33 16515.5 34 18834.0 35 20608.5 36 23056.5 37 25694.5 38 27498.5 39 28094.5 40 27741.0 41 28429.0 42 28910.0 43 30031.5 44 32400.0 45 33491.0 46 33119.5 47 34525.5 48 39701.5 49 39608.5 50 34859.0 51 32836.0 52 31331.5 53 30750.0 54 29758.0 55 27277.0 56 24821.0 57 22378.5 58 21060.0 59 19426.0 60 16224.5 61 12997.5 62 10141.5 63 7804.0 64 5659.5 65 4121.0 66 3019.0 67 2297.5 68 1592.0 69 992.0 70 727.5 71 499.5 72 323.0 73 245.0 74 196.0 75 168.0 76 124.5 77 54.0 78 29.0 79 17.5 80 12.0 81 8.0 82 5.5 83 6.0 84 4.5 85 2.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015881246141435643 2 0.0038914311737292635 3 0.0028396930186673007 4 2.1034763101239262E-4 5 0.002418997756642515 6 3.1552144651858895E-4 7 0.0 8 0.0 9 0.0 10-11 1.0517381550619631E-4 12-13 6.83629800790276E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0035759097272106744 22-23 0.0 24-25 0.003523322819457576 26-27 0.0015776072325929449 28-29 0.0031026275574327915 30-31 0.01514502943289227 32-33 8.413905240495705E-4 34-35 0.002681932295408006 36-37 0.0034707359117044784 38-39 0.002313823941136319 40-41 0.00515351695980362 42-43 0.004154365712494754 44-45 4.2069526202478523E-4 46-47 0.0 48-49 2.629345387654908E-4 50-51 1.5776072325929448E-4 52-53 0.001314672693827454 54-55 0.00483799551328503 56-57 4.7328216977788343E-4 58-59 0.0022086501256301226 60-61 0.0014198465093336503 62-63 0.0 64-65 2.629345387654908E-4 66-67 5.2586907753098154E-5 68-69 5.2586907753098154E-5 70-71 3.681083542716871E-4 72-73 0.0 74-75 4.2069526202478523E-4 76-77 9.99151247308865E-4 78-79 3.1552144651858895E-4 80-81 0.00683629800790276 82-83 0.0022086501256301226 84-85 0.00515351695980362 86-87 0.0022086501256301226 88-89 0.0011043250628150613 90-91 3.1552144651858895E-4 92-93 5.2586907753098154E-5 94-95 4.7328216977788343E-4 96-97 0.002313823941136319 98-99 0.003312975188445184 100-101 0.0012620857860743558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 950807.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.143713802791666 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41255371928082 38.56878064556551 2 13.39607156131373 13.702497000270919 3 4.5423199596179025 6.969333360462185 4 2.098963173147535 4.293950872402279 5 1.1609732326284288 2.968824137112512 6 0.6939266575628803 2.1293991824714262 7 0.46444733440989805 1.6627493083270513 8 0.33724684324422205 1.379844482542195 9 0.2524381693763531 1.1619562938736365 >10 1.4464517484081996 13.775536083303047 >50 0.13243575649930678 4.614551062737761 >100 0.05802705491625733 5.276392539358975 >500 0.0029013527161523495 1.0728304398707813 >1k 8.289579189006713E-4 0.9847527777948457 >5k 4.1447895945033567E-4 1.4386018139069465 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7684 0.8081555983496126 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5889 0.6193685995159901 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3857 0.4056554064073992 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2827 0.29732637643601695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1374 0.14450882250551375 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1233 0.12967931451914005 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 994 0.10454277261315913 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 991 0.10422725116664056 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.0517381550619632E-4 0.0 0.0 0.0 0.0 7 1.0517381550619632E-4 0.0 0.0 0.0 0.0 8 1.0517381550619632E-4 0.0 0.0 0.0 0.0 9 1.0517381550619632E-4 0.0 0.0 0.0 0.0 10-11 1.0517381550619632E-4 0.0 0.0 0.0 0.0 12-13 1.0517381550619632E-4 0.0 0.0 0.0 1.0517381550619632E-4 14-15 3.1552144651858895E-4 0.0 0.0 0.0 1.0517381550619632E-4 16-17 3.681083542716871E-4 0.0 0.0 0.0 1.0517381550619632E-4 18-19 5.258690775309816E-4 0.0 0.0 0.0 1.0517381550619632E-4 20-21 6.310428930371779E-4 0.0 0.0 5.258690775309816E-5 1.0517381550619632E-4 22-23 6.310428930371779E-4 0.0 0.0 2.1034763101239264E-4 1.0517381550619632E-4 24-25 7.362167085433742E-4 0.0 0.0 3.681083542716871E-4 1.0517381550619632E-4 26-27 7.362167085433742E-4 0.0 0.0 6.836298007902762E-4 1.0517381550619632E-4 28-29 9.465643395557669E-4 0.0 0.0 0.002892279926420399 1.0517381550619632E-4 30-31 9.465643395557669E-4 0.0 0.0 0.0058897336683469935 1.0517381550619632E-4 32-33 9.465643395557669E-4 0.0 0.0 0.010675142273878925 1.0517381550619632E-4 34-35 0.0011043250628150613 0.0 0.0 0.017090745019756902 1.0517381550619632E-4 36-37 0.0012620857860743558 0.0 0.0 0.02340117395012868 1.0517381550619632E-4 38-39 0.001367259601580552 0.0 0.0 0.027871061109142024 1.0517381550619632E-4 40-41 0.001367259601580552 0.0 0.0 0.0314469708363527 1.0517381550619632E-4 42-43 0.0014724334170867484 0.0 0.0 0.036600487796156315 1.0517381550619632E-4 44-45 0.0015776072325929447 0.0 0.0 0.042385047648997115 1.0517381550619632E-4 46-47 0.00199830249461773 0.0 0.0 0.051482582690283094 1.0517381550619632E-4 48-49 0.00199830249461773 0.0 0.0 0.06205255114865583 1.0517381550619632E-4 50-51 0.00199830249461773 0.0 0.0 0.07041386948139844 1.0517381550619632E-4 52-53 0.00199830249461773 0.0 0.0 0.08687357160811815 1.0517381550619632E-4 54-55 0.00199830249461773 0.0 0.0 0.1255775357143984 1.0517381550619632E-4 56-57 0.0021034763101239265 0.0 0.0 0.1877878475863135 1.0517381550619632E-4 58-59 0.0021034763101239265 0.0 0.0 0.2600948457468235 1.0517381550619632E-4 60-61 0.0021034763101239265 0.0 0.0 0.32372500412807226 1.0517381550619632E-4 62-63 0.0021034763101239265 0.0 0.0 0.40597092785391775 1.0517381550619632E-4 64-65 0.0021034763101239265 0.0 0.0 0.5062015740313228 1.0517381550619632E-4 66-67 0.002313823941136319 0.0 0.0 0.6689580535271616 1.0517381550619632E-4 68-69 0.002313823941136319 0.0 0.0 0.8687883029889347 1.0517381550619632E-4 70-71 0.002313823941136319 0.0 0.0 1.030861152683983 1.0517381550619632E-4 72-73 0.0023664108488894173 0.0 0.0 1.2041876006381949 1.0517381550619632E-4 74-75 0.002418997756642515 0.0 0.0 1.3851917371243587 1.0517381550619632E-4 76-77 0.002418997756642515 0.0 0.0 1.6006402981888017 1.0517381550619632E-4 78-79 0.002418997756642515 0.0 0.0 1.748725030421526 1.0517381550619632E-4 80-81 0.0025241715721487116 0.0 0.0 1.8688335277296022 1.0517381550619632E-4 82-83 0.0025241715721487116 0.0 0.0 2.040003912465937 1.0517381550619632E-4 84-85 0.0025241715721487116 0.0 0.0 2.3504770158402284 1.0517381550619632E-4 86-87 0.0025241715721487116 0.0 0.0 2.774590426868965 1.0517381550619632E-4 88-89 0.0025241715721487116 0.0 0.0 3.2737453552613727 1.0517381550619632E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9975 0.0 28.7195 1 GTACATG 5350 0.0 25.663538 1 GGTATCA 6940 0.0 25.26035 1 GAGTACT 6055 0.0 24.199104 12-13 ATCAACG 11845 0.0 24.13902 3 TCAACGC 11830 0.0 24.089333 4 CAACGCA 11890 0.0 23.967772 5 TATCAAC 12055 0.0 23.759169 2 TACATGG 5690 0.0 23.624102 2 AACGCAG 12085 0.0 23.581036 6 ATTCCGT 165 2.4308665E-6 23.028429 6 ACATGGG 5475 0.0 22.988997 3 AGTACTT 6295 0.0 22.861523 12-13 GTACTTT 6560 0.0 22.44482 14-15 AGAGTAC 9290 0.0 22.290974 10-11 CATGGGG 1985 0.0 22.013323 4 TATTCCG 130 2.942609E-4 21.921295 5 ACGCAGA 13120 0.0 21.575989 7 CGCAGAG 13065 0.0 21.521402 8 CCGTTGC 135 3.7973654E-4 21.109392 9 >>END_MODULE