FastQCFastQC Report
Thu 26 May 2016
SRR936934_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936934_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823051
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.6686098431324425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39960.4855106184185427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.3180847845394757No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18990.23072689298719035No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14400.17495878141208746No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10500.12757411144631378No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.10776974938369553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73150.027.8668251
TAGGCGG700.0083710327.1555945
GTACATG47150.026.3028831
GGTATCA51650.025.2992061
TACATGG48000.025.1456812
CGTATAG950.001223776425.0086881
ACATGGG47400.024.8609583
CATGGGG16100.024.7912244
ATGGGGA9050.023.104765
GTTCTAG1652.4211622E-623.038311
TCAACGC89400.022.6420944
GTACTAG1050.002191263722.6269111
ATCAACG90050.022.5314253
AGTACTT42750.022.4563412-13
CAACGCA90200.022.3386365
TATCAAC90850.022.2820722
AACGCAG91350.022.1601146
GAGTACT40350.021.96640812-13
AGAGTAC68650.021.9473510-11
CATGGGA29900.021.4539434
Produced by FastQC (version 0.11.5)