FastQCFastQC Report
Thu 26 May 2016
SRR936934_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936934_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823051
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69710.846970600849765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55320.672133318591436No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35290.4287705136133727No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26460.32148676084471073No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12920.15697690665584513No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11200.13607905220940136No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9120.1108072282276554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.028.7619531
GTATCAA86650.028.3507651
GTACATG48000.023.362191
ATCAACG103800.023.2461383
TATCAAC104450.023.2379022
TCAACGC104400.023.1552224
CAACGCA104800.023.0215265
GAGTACT56050.022.92046212-13
AACGCAG106750.022.6441086
ATGGGAG13150.022.031085
TACATGG50050.021.8277112
AGTACTT57950.021.5952812-13
ACATGGG49450.021.4201743
GTACTTT60350.021.20930114-15
AGAGTAC85900.021.0091710-11
CATGGGA30550.020.8317264
CGATACA1150.003714989920.6592711
CCGATCA1150.003726780920.6479769
ACGCAGA117200.020.4629887
TCGCTTA704.8496772E-420.35918872-73
Produced by FastQC (version 0.11.5)