##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936934_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 823051 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.361662886017996 33.0 31.0 34.0 31.0 34.0 2 32.4266539983549 34.0 31.0 34.0 31.0 34.0 3 32.549485997830025 34.0 31.0 34.0 31.0 34.0 4 36.20951192574944 37.0 35.0 37.0 35.0 37.0 5 36.14325357723884 37.0 35.0 37.0 35.0 37.0 6 36.19756005399422 37.0 36.0 37.0 35.0 37.0 7 36.15677400306907 37.0 36.0 37.0 35.0 37.0 8 36.144370154461875 37.0 36.0 37.0 35.0 37.0 9 37.86237547855479 39.0 38.0 39.0 35.0 39.0 10-11 37.76871967836745 39.0 37.0 39.0 35.0 39.0 12-13 37.70042135906523 39.0 37.0 39.0 35.0 39.0 14-15 38.92583084158819 40.0 38.0 41.0 36.0 41.0 16-17 38.869387194718186 40.0 38.0 41.0 35.5 41.0 18-19 38.94246468323348 40.0 38.0 41.0 35.5 41.0 20-21 39.032495556168456 40.0 38.0 41.0 36.0 41.0 22-23 38.96654095554224 40.0 38.0 41.0 35.0 41.0 24-25 38.872873005439516 40.0 38.0 41.0 35.0 41.0 26-27 38.70400133163073 40.0 38.0 41.0 35.0 41.0 28-29 38.546696377259735 40.0 38.0 41.0 34.5 41.0 30-31 38.44541103771212 40.0 38.0 41.0 34.5 41.0 32-33 38.30960839607752 40.0 38.0 41.0 34.0 41.0 34-35 38.16139704586958 40.0 37.5 41.0 34.0 41.0 36-37 37.96989919215213 40.0 37.0 41.0 34.0 41.0 38-39 37.80828648528463 40.0 37.0 41.0 33.0 41.0 40-41 37.56956434048437 40.0 37.0 41.0 33.0 41.0 42-43 37.290508121610934 40.0 37.0 41.0 33.0 41.0 44-45 36.93632715348137 40.0 36.0 41.0 31.5 41.0 46-47 36.42053894594624 40.0 36.0 41.0 30.5 41.0 48-49 35.916559848660654 39.0 36.0 41.0 29.5 41.0 50-51 35.638956152170394 39.0 35.0 41.0 28.0 41.0 52-53 35.41928568217522 39.0 35.0 40.5 27.5 41.0 54-55 35.144543898251754 39.0 35.0 40.0 26.5 41.0 56-57 34.88011982246543 38.0 35.0 40.0 26.0 41.0 58-59 34.53789923103186 38.0 34.0 40.0 25.5 41.0 60-61 34.18596781973413 38.0 34.0 40.0 25.0 41.0 62-63 33.860768652246335 37.0 34.0 40.0 23.5 41.0 64-65 33.45841326965157 36.5 33.0 39.5 22.5 41.0 66-67 33.02618185264339 36.0 33.0 39.0 21.5 40.0 68-69 32.57764828667968 35.5 32.5 39.0 20.0 40.0 70-71 32.188522946937674 35.0 32.0 38.0 19.0 40.0 72-73 31.813630018066924 35.0 32.0 37.5 18.0 39.5 74-75 31.348364196143372 35.0 31.0 37.0 14.5 39.0 76-77 29.92468814204709 33.5 29.5 35.5 11.0 37.5 78-79 30.586063318068987 34.0 31.0 36.0 7.5 38.0 80-81 30.253428402371178 34.0 31.0 35.5 7.0 37.0 82-83 30.096667764209023 34.0 31.0 35.0 4.0 37.0 84-85 29.80127233913816 34.0 30.0 35.0 2.0 36.5 86-87 29.46481749004618 34.0 30.0 35.0 2.0 36.0 88-89 29.11357558644604 34.0 30.0 35.0 2.0 36.0 90-91 28.700858756018764 34.0 29.0 35.0 2.0 35.0 92-93 28.320384156024353 34.0 29.0 35.0 2.0 35.0 94-95 27.880552966948585 34.0 29.0 35.0 2.0 35.0 96-97 27.299073204455134 33.0 27.0 35.0 2.0 35.0 98-99 26.61020033995463 33.0 25.5 35.0 2.0 35.0 100-101 23.547101576937514 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1636814049209363 1101 2 0.022639145975915653 1101 3 0.09750460283605378 1101 4 0.044268487434777626 1101 5 0.10622510652153494 1101 6 0.11863798109874324 1101 7 0.05231451957629929 1101 8 0.017457660633361627 1101 9 0.12792125502094365 1101 10-11 0.08876728657642019 1101 12-13 0.03769535091618792 1101 14-15 0.16210403898178072 1101 16-17 0.045974502302321696 1101 18-19 0.031076240497988294 1101 20-21 0.14082005720812418 1101 22-23 0.054536652292590304 1101 24-25 -0.005764686661571261 1101 26-27 0.09328809561385043 1101 28-29 0.18477007913440957 1101 30-31 0.11115919136016572 1101 32-33 0.2611363187754421 1101 34-35 0.3557531098951401 1101 36-37 0.40640531048737927 1101 38-39 0.39253005576568967 1101 40-41 0.5573464160456538 1101 42-43 0.5975700944830677 1101 44-45 0.5775727166330853 1101 46-47 0.3847843191475562 1101 48-49 0.41142327144560653 1101 50-51 0.5681405989292827 1101 52-53 0.5250221809989455 1101 54-55 0.5800730682951141 1101 56-57 0.579192449775455 1101 58-59 0.6432365308774521 1101 60-61 0.522091959648435 1101 62-63 0.4798203229691538 1101 64-65 0.5592632532368924 1101 66-67 0.5296343714681129 1101 68-69 0.4097022462087452 1101 70-71 0.4637830681180546 1101 72-73 0.6615742066823564 1101 74-75 0.42751710531037546 1101 76-77 0.16831250766882278 1101 78-79 0.6539087662200203 1101 80-81 0.7907159753469024 1101 82-83 0.6426893634090156 1101 84-85 0.6026449353346912 1101 86-87 0.5622628937230907 1101 88-89 0.3743945517841176 1101 90-91 0.4059322672209049 1101 92-93 0.44321531344850484 1101 94-95 0.43583762959054795 1101 96-97 0.35463045103298896 1101 98-99 0.46203822638210923 1101 100-101 0.12426612153024053 1102 1 0.22210083479057374 1102 2 0.2105279326909013 1102 3 0.097139481399374 1102 4 0.12863858534141315 1102 5 0.18822191508971997 1102 6 0.22383352391261013 1102 7 0.25852023169832705 1102 8 0.2115873193124358 1102 9 0.23864771144978647 1102 10-11 0.2609524707599036 1102 12-13 0.338420184583093 1102 14-15 0.3162934693923418 1102 16-17 0.40659615535581395 1102 18-19 0.37050056569948 1102 20-21 0.2288177042033155 1102 22-23 0.250644052087047 1102 24-25 0.24435025897590634 1102 26-27 0.3894725766004896 1102 28-29 0.3748834020099494 1102 30-31 0.37505070972441246 1102 32-33 0.35261276168126443 1102 34-35 0.3900914287137667 1102 36-37 0.45947976821442893 1102 38-39 0.4464835095440165 1102 40-41 0.6104749658374047 1102 42-43 0.5081288655113667 1102 44-45 0.4796119874720475 1102 46-47 0.41739845340406845 1102 48-49 0.5300471693197863 1102 50-51 0.4640911317361258 1102 52-53 0.24246551956117202 1102 54-55 0.23110149367511212 1102 56-57 0.30887749872876924 1102 58-59 0.34857000845629216 1102 60-61 0.33197187526481997 1102 62-63 0.15657290586523942 1102 64-65 0.29133511454483596 1102 66-67 0.3508093863434283 1102 68-69 0.2565202805031177 1102 70-71 0.19795775844097818 1102 72-73 0.2836126661403533 1102 74-75 0.5619595513535423 1102 76-77 0.6408810693608231 1102 78-79 0.5571177779579983 1102 80-81 0.5113913147746096 1102 82-83 0.47705269980756526 1102 84-85 0.42706209333760725 1102 86-87 0.30071423791089913 1102 88-89 0.4968657477596672 1102 90-91 0.3839708889550977 1102 92-93 0.32148450260845607 1102 94-95 0.3279645295743201 1102 96-97 0.2991286119027876 1102 98-99 0.3404426457037957 1102 100-101 0.6762114074068037 1103 1 0.1577224836093052 1103 2 0.0545018141285496 1103 3 0.10600837793141693 1103 4 0.05251326430387593 1103 5 0.07710350293009327 1103 6 0.09898747173556188 1103 7 0.0579712762906297 1103 8 0.1100887686748635 1103 9 0.14724213027798072 1103 10-11 0.2339740534937178 1103 12-13 0.21473437331561485 1103 14-15 0.14573711697948966 1103 16-17 0.14987595740144144 1103 18-19 0.1148091897814183 1103 20-21 0.09842577557503063 1103 22-23 0.0831863689642276 1103 24-25 0.10776286918001432 1103 26-27 -0.06252386382546149 1103 28-29 0.024648349968074967 1103 30-31 0.07204947814183527 1103 32-33 0.016909820902370143 1103 34-35 0.08560422515719068 1103 36-37 0.2040089882621885 1103 38-39 0.029042723133478887 1103 40-41 0.15887372073745354 1103 42-43 -0.09600557224114681 1103 44-45 -0.011309784504938136 1103 46-47 0.21508848509478895 1103 48-49 -0.04387500817829704 1103 50-51 -0.06200208554808739 1103 52-53 -0.05345035734064396 1103 54-55 0.164428005743126 1103 56-57 0.08083571503672715 1103 58-59 0.1870111553300262 1103 60-61 0.15578704025182333 1103 62-63 0.08850637787677584 1103 64-65 0.07101137353764386 1103 66-67 0.12617792241227477 1103 68-69 0.04448531557318347 1103 70-71 0.13524185600635263 1103 72-73 0.3432261444600009 1103 74-75 0.2552832848510853 1103 76-77 0.2434994965397479 1103 78-79 0.5049210176209655 1103 80-81 0.6737743519774142 1103 82-83 0.5995857798153743 1103 84-85 0.4787388104190562 1103 86-87 0.4392475657371584 1103 88-89 0.42728414790385116 1103 90-91 0.42969339237198767 1103 92-93 0.49718308779041465 1103 94-95 0.43116683203298223 1103 96-97 0.6463213170288782 1103 98-99 0.7651809344047784 1103 100-101 0.8802270049496457 1104 1 0.20929650198563365 1104 2 0.1351418009183547 1104 3 0.02449793362064412 1104 4 0.05039609458825112 1104 5 -0.01798159667713861 1104 6 -0.020320644278257305 1104 7 0.08753498599658371 1104 8 0.09946989460812716 1104 9 0.15254743912527147 1104 10-11 0.10968026818298426 1104 12-13 0.0854195342558981 1104 14-15 0.0877608717416436 1104 16-17 0.16538319352189745 1104 18-19 0.10708299467155058 1104 20-21 0.2148488792632648 1104 22-23 0.10888491702221614 1104 24-25 0.09866375732976707 1104 26-27 0.18565088818850484 1104 28-29 0.12571460145716884 1104 30-31 0.18929998826768468 1104 32-33 0.13175300880561025 1104 34-35 0.18815721337417557 1104 36-37 0.11791979585333223 1104 38-39 0.2453119044552139 1104 40-41 0.41100386475216766 1104 42-43 0.12141891144072048 1104 44-45 0.08011243457200123 1104 46-47 0.06396797744584859 1104 48-49 0.28630910892064776 1104 50-51 0.14477285754191627 1104 52-53 0.09233240571327173 1104 54-55 0.26918780428764677 1104 56-57 0.2420726375329778 1104 58-59 0.05690842247084049 1104 60-61 0.12760096521319753 1104 62-63 0.3534020527172501 1104 64-65 0.4698814300348175 1104 66-67 0.3229078092999842 1104 68-69 0.2640234305254694 1104 70-71 0.21690209449756281 1104 72-73 0.3761572745214501 1104 74-75 0.2424888567228045 1104 76-77 0.4969418139854582 1104 78-79 0.44703618221764074 1104 80-81 0.6183528641791547 1104 82-83 0.5446349052296675 1104 84-85 0.10383169460574848 1104 86-87 0.20227553124911068 1104 88-89 0.4889038122233522 1104 90-91 0.35563491366753297 1104 92-93 0.3706111335071256 1104 94-95 0.48755431537280103 1104 96-97 0.6144240027087875 1104 98-99 0.7118465395378806 1104 100-101 0.9610338884050549 1105 1 0.14450985134261884 1105 2 0.10451176762784087 1105 3 0.07529289998479527 1105 4 0.17681715739819026 1105 5 0.17783291577572413 1105 6 0.14552135702020763 1105 7 0.1375329139730468 1105 8 -0.04668650172853006 1105 9 -0.07107639190811454 1105 10-11 0.09216476254017181 1105 12-13 0.10727869190926498 1105 14-15 0.16656545229501063 1105 16-17 0.23592523487948114 1105 18-19 0.09471715819564963 1105 20-21 0.13799476353384676 1105 22-23 -0.164660867791234 1105 24-25 0.019259671299344916 1105 26-27 0.18617580081818375 1105 28-29 0.0935954739172189 1105 30-31 -0.00373110589887915 1105 32-33 -0.1952502926185673 1105 34-35 -4.5908930702154294E-4 1105 36-37 -0.03113605208776704 1105 38-39 0.19397475859856428 1105 40-41 0.12172037049167272 1105 42-43 0.12932506258793097 1105 44-45 0.10220987493225664 1105 46-47 0.09681921996929788 1105 48-49 0.07748366999700096 1105 50-51 -0.02640046032030341 1105 52-53 0.13586009485448614 1105 54-55 0.24645632472469714 1105 56-57 0.12025284832684946 1105 58-59 0.17600488207329335 1105 60-61 -0.03949806369843856 1105 62-63 -0.1287529898973716 1105 64-65 -0.013951951645729821 1105 66-67 -0.06702077495540237 1105 68-69 0.1631973838082459 1105 70-71 0.09963385033030647 1105 72-73 0.020532421289782832 1105 74-75 -0.025416880019697885 1105 76-77 -0.15585403644206863 1105 78-79 0.010096837253350088 1105 80-81 0.28459891006756877 1105 82-83 0.32586582534625563 1105 84-85 0.18763468660773697 1105 86-87 -0.01513222916971202 1105 88-89 -0.21449706907732846 1105 90-91 -0.08103852160936142 1105 92-93 0.09456786965564845 1105 94-95 -0.09210393887829937 1105 96-97 0.10278751851832624 1105 98-99 0.36565768149911904 1105 100-101 0.6071833779965914 1106 1 0.08956486356755278 1106 2 -0.0012706284601762263 1106 3 0.005295956219512732 1106 4 0.0017590889385346031 1106 5 0.0987345050177808 1106 6 0.02566805628671176 1106 7 -0.039753149596634785 1106 8 -0.03353857996814469 1106 9 0.0701674956224494 1106 10-11 0.05584905349371638 1106 12-13 -0.05947100246726933 1106 14-15 0.10832584349305563 1106 16-17 0.18370946470833616 1106 18-19 0.13836477433256533 1106 20-21 -0.06785945407006722 1106 22-23 0.0162330808640192 1106 24-25 -0.01101844606007063 1106 26-27 -0.08510476152900992 1106 28-29 0.08947872575095062 1106 30-31 0.06339603346543043 1106 32-33 0.015548912760401379 1106 34-35 0.09917416252671529 1106 36-37 0.053463581164074014 1106 38-39 0.009118401630352935 1106 40-41 -0.01788384711223756 1106 42-43 -0.07677671002820574 1106 44-45 -0.09289250893082368 1106 46-47 -0.17798823724341872 1106 48-49 -0.19420642780251285 1106 50-51 -0.2748387243789878 1106 52-53 -0.3498321423093316 1106 54-55 -0.3148517437349554 1106 56-57 -0.4651872494726703 1106 58-59 -0.43778790521277244 1106 60-61 -0.513814995238782 1106 62-63 -0.4014531732714559 1106 64-65 -0.46211362646235443 1106 66-67 -0.33293611725368777 1106 68-69 -0.29859662597170455 1106 70-71 -0.31491993940074536 1106 72-73 -0.3582848158740326 1106 74-75 -0.2568578946896274 1106 76-77 -0.29884914646651595 1106 78-79 -0.2573741285168225 1106 80-81 0.03615300542208999 1106 82-83 0.11335665664209671 1106 84-85 0.21435076240235773 1106 86-87 0.32680236740729995 1106 88-89 -0.19564774144896901 1106 90-91 -0.04804799593699016 1106 92-93 -0.02709013767931623 1106 94-95 0.45552747921460934 1106 96-97 0.513629511183364 1106 98-99 0.14882394066782467 1106 100-101 0.2968319110039275 1107 1 0.2061352153263485 1107 2 0.12644293937901807 1107 3 0.016809775314989395 1107 4 0.12970506883803523 1107 5 0.158979479892146 1107 6 0.11596956382440027 1107 7 0.16682976497622803 1107 8 0.12775538988110213 1107 9 0.18408081220535877 1107 10-11 0.15231576203643016 1107 12-13 0.1962796256734336 1107 14-15 0.16809971283979053 1107 16-17 0.10352730390431475 1107 18-19 0.2561631411667449 1107 20-21 0.14043514391988055 1107 22-23 0.20013069392934568 1107 24-25 0.2702441167540002 1107 26-27 0.305554786209683 1107 28-29 0.2780277207258308 1107 30-31 0.24301914904331312 1107 32-33 0.13201562130311828 1107 34-35 0.2009329564965654 1107 36-37 0.12450001332486949 1107 38-39 0.39124779861527514 1107 40-41 0.45456276092796344 1107 42-43 0.2288231643436589 1107 44-45 -0.0020476596845924178 1107 46-47 0.05245774265608105 1107 48-49 0.20445563249899124 1107 50-51 0.2978936876813165 1107 52-53 0.14987577226353466 1107 54-55 -0.03934923117214595 1107 56-57 -0.15666966153296613 1107 58-59 -0.010884638881115904 1107 60-61 0.11188538164564221 1107 62-63 0.23518313326120932 1107 64-65 -0.008762495809094162 1107 66-67 0.13962983752017522 1107 68-69 0.02733930367654125 1107 70-71 0.05670066361432902 1107 72-73 -0.09422074552227144 1107 74-75 -0.03479445247856816 1107 76-77 -0.033346633903704515 1107 78-79 3.614242469964779E-4 1107 80-81 0.08989986471857137 1107 82-83 0.10118957121495953 1107 84-85 -0.08217247880367395 1107 86-87 -0.08268883862284682 1107 88-89 -0.27349007813238657 1107 90-91 -0.23265540799728868 1107 92-93 -0.22858825325720034 1107 94-95 -0.08363709364971328 1107 96-97 0.2300082800275831 1107 98-99 0.32173851353214644 1107 100-101 0.11603417231045654 1108 1 0.09251531401800861 1108 2 0.1371399249503753 1108 3 0.12170361387717321 1108 4 0.06464036306980603 1108 5 0.16757781261108562 1108 6 0.14028839090704537 1108 7 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56-57 -1.1578971731367886 2316 58-59 -1.161576073151707 2316 60-61 -0.8233099516182776 2316 62-63 -0.9494936585495353 2316 64-65 -0.8608315926564671 2316 66-67 -0.8731521750508549 2316 68-69 -1.0457517513806422 2316 70-71 -0.7209368423451323 2316 72-73 -0.8032998104214677 2316 74-75 -0.8050346940353172 2316 76-77 -0.7060956023007563 2316 78-79 -0.7790282997272904 2316 80-81 -0.4998482214045161 2316 82-83 -0.9148208242739315 2316 84-85 -1.1891927217852114 2316 86-87 -0.9966068474236671 2316 88-89 -0.9687904622777559 2316 90-91 -1.020415089721066 2316 92-93 -0.7859716807545567 2316 94-95 -0.8506407323448251 2316 96-97 -0.6485262957959179 2316 98-99 -0.9333407267258487 2316 100-101 -1.6882948610789548 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 8.0 6 37.0 7 77.0 8 161.0 9 404.0 10 762.0 11 1255.0 12 2125.0 13 3147.0 14 3903.0 15 5501.0 16 8228.0 17 12293.0 18 10955.0 19 5508.0 20 4193.0 21 4059.0 22 4023.0 23 4526.0 24 4921.0 25 5696.0 26 6721.0 27 8237.0 28 10355.0 29 13048.0 30 16450.0 31 20928.0 32 27136.0 33 36904.0 34 52396.0 35 78818.0 36 124688.0 37 191766.0 38 142764.0 39 11053.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1778815888934 19.647036805685524 15.330638673313388 20.844442932107693 2 15.630289267093636 21.79642799427954 39.627902907737564 22.94537983088926 3 19.122348472421198 26.753012567909607 30.74648012258056 23.378158837088638 4 12.8843777603089 17.865843064403055 37.85451934327278 31.39525983201527 5 13.14570248566523 38.29360667509924 33.6703932811583 14.89029755807723 6 30.23312042631623 37.43619775688262 17.393697352897938 14.936984463903208 7 27.154210370924766 34.60137950139177 21.181433471315874 17.06297665636759 8 26.72680882084928 33.57548144098171 19.984569588724867 19.713140149444143 9 27.4963519879084 16.47856572678971 20.788505208061224 35.23657707724065 10-11 24.74597564427964 27.25584441304366 26.985144298469958 21.013035644206738 12-13 26.609169077418233 25.10482364962475 27.787349234371224 20.498658038585795 14-15 23.44071023545321 25.487424230090237 27.54750313164069 23.524362402815864 16-17 21.228722434846002 29.023206366563393 27.77388980013365 21.97418139845696 18-19 21.07825328338641 26.749225705249163 31.693715849318416 20.478805162046008 20-21 22.333099240128597 25.473370480441876 31.77547950631444 20.418050773115084 22-23 21.9117041349807 25.691239060519944 31.73484996676998 20.662206837729375 24-25 21.835299806987692 25.748887156753092 31.930870365836395 20.484942670422825 26-27 21.540262650122237 25.858813408570636 32.00975460391059 20.591169337396536 28-29 21.08825600419934 26.2198104100128 32.00957731384415 20.682356271943707 30-31 21.223717154038745 26.006040496852133 31.765842631079998 21.00439971802912 32-33 21.294669543791098 26.09289418305704 31.62431170144002 20.988124571711843 34-35 21.81001006060665 25.934251262436025 31.607801587340163 20.647937089617162 36-37 21.815764674001212 25.751373632215934 31.423078722508368 21.009782971274486 38-39 21.559708551768637 26.070193750011388 31.648670372244737 20.721427325975235 40-41 21.64797316057662 26.092493212073126 31.40126262426812 20.858271003082134 42-43 21.460462883926645 26.49477364617539 30.767473579649874 21.27728989024809 44-45 21.787557448307915 26.253899498494874 30.516601915500484 21.44194113769672 46-47 22.253873309385426 26.72208340699349 29.829843512408015 21.194199771213064 48-49 22.19426344137627 27.122292193425086 29.16302669260313 21.52041767259552 50-51 22.034319443229425 27.316070335053798 29.18100389778283 21.46860632393394 52-53 22.371333937610416 27.693488144909857 28.46485270138587 21.47032521609386 54-55 21.854530741780707 27.491607262406248 28.640771390697576 22.01309060511547 56-57 22.239596812309472 27.707246662073942 28.383093916341956 21.67006260927463 58-59 22.77387658794019 27.058543362742615 28.451611374666708 21.715968674650487 60-61 22.231942166332544 27.31279995140028 29.197375615090216 21.257882267176964 62-63 22.05831966466193 27.169734524026484 29.404774922544195 21.36717088876739 64-65 21.9967252854743 27.44897308233264 28.979400891281298 21.574900740911758 66-67 22.071649831813467 27.580890219180134 28.57257414392539 21.774885805081013 68-69 22.03025739379142 27.357164944288755 28.829574801178804 21.78300286074102 70-71 22.168260409958567 27.410298781302778 28.749772177737814 21.671668631000838 72-73 22.13579798357845 27.292412718742366 28.768517382127317 21.803271915551868 74-75 22.017746104939565 28.096257886567095 28.177728364909978 21.708267643583365 76-77 22.158162716679254 27.67133707038533 28.28122541446466 21.889274798470755 78-79 22.125842838620933 27.673844389115914 28.160257508539267 22.040055263723886 80-81 22.219049742391263 27.93261166842132 27.927083453566944 21.921255135620477 82-83 22.179060426985856 27.687373595309367 28.10237891425471 22.031187063450066 84-85 21.828731322429125 27.975532351776206 27.888234679471136 22.307501646323537 86-87 21.98296338866 27.814376022871002 27.908477117456876 22.294183471012126 88-89 21.971876163751755 27.733818041318848 27.44112213462675 22.853183660302648 90-91 21.86688034564478 27.572485713053354 27.828244383788668 22.7323895575132 92-93 22.012635927343418 27.610412490128184 27.805236619889435 22.571714962638964 94-95 21.85546893984312 27.40040678064949 27.827903111361667 22.91622116814572 96-97 21.85493546848754 27.563475984071395 27.808419319662598 22.77316922777847 98-99 21.68604580523662 28.149018893141363 27.210679788591214 22.954255513030798 100-101 21.29446142069996 28.29638719632584 27.244986604620646 23.164164778353552 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 536.0 1 381.0 2 265.5 3 329.5 4 313.5 5 323.0 6 503.5 7 974.0 8 1652.5 9 2074.5 10 1939.5 11 1708.5 12 1755.5 13 1909.0 14 2049.0 15 2170.0 16 2211.0 17 2243.0 18 2343.0 19 2425.5 20 2524.5 21 2754.5 22 3069.5 23 3403.0 24 3763.0 25 4317.0 26 5123.0 27 6194.0 28 7221.5 29 8270.5 30 9637.0 31 11170.5 32 12911.5 33 14519.0 34 16297.5 35 18026.5 36 20543.0 37 23025.0 38 24391.5 39 24789.0 40 24317.0 41 24502.5 42 24992.0 43 26176.0 44 28295.0 45 29316.0 46 28761.5 47 29999.0 48 34355.0 49 34027.5 50 29858.0 51 27831.0 52 26519.0 53 25892.0 54 24926.5 55 23080.0 56 21005.5 57 18861.0 58 17739.0 59 16399.0 60 13741.0 61 10990.0 62 8650.0 63 6498.0 64 4573.5 65 3397.0 66 2516.5 67 1916.0 68 1352.0 69 850.0 70 591.0 71 392.5 72 266.0 73 193.0 74 146.5 75 126.0 76 94.0 77 46.0 78 24.5 79 12.0 80 10.0 81 9.5 82 7.0 83 3.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02296334006033648 2 0.004859966150335763 3 0.009962930608188314 4 0.0 5 0.006317955995436491 6 0.0 7 0.0 8 1.2149915375839409E-4 9 0.0 10-11 0.0 12-13 2.4299830751678818E-4 14-15 0.0 16-17 1.2149915375839409E-4 18-19 0.005345962765369339 20-21 0.0020654856138926994 22-23 0.0 24-25 0.0056497106497653245 26-27 0.006682453456711673 28-29 0.007836695417416417 30-31 0.0329262706685248 32-33 8.504940763087584E-4 34-35 0.004738466996577369 36-37 0.017192130256812762 38-39 0.006986201341107659 40-41 0.010023680185067512 42-43 0.007107700494866054 44-45 0.0034627258821142313 46-47 0.001761737729496714 48-49 0.0017009881526175168 50-51 0.0023084839214094874 52-53 0.003523475458993428 54-55 0.0021869847676510936 56-57 0.0012149915375839408 58-59 0.001397240268221532 60-61 1.2149915375839409E-4 62-63 1.2149915375839409E-4 64-65 0.009537683570033935 66-67 7.897444994295614E-4 68-69 0.001518739421979926 70-71 0.004981465304094157 72-73 0.01312190860590656 74-75 0.006500204726074083 76-77 0.003827223343389413 78-79 0.010691925530738678 80-81 6.0749576879197044E-5 82-83 0.006014208111040507 84-85 6.074957687919704E-4 86-87 0.0 88-89 0.001518739421979926 90-91 0.0011542419607047437 92-93 1.2149915375839409E-4 94-95 3.6449746127518225E-4 96-97 4.252470381543792E-4 98-99 1.2149915375839409E-4 100-101 7.289949225503645E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 823051.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.503490483210825 #Duplication Level Percentage of deduplicated Percentage of total 1 76.62768800800546 40.23221088078762 2 12.692430359470485 13.327937931745495 3 4.3790914101212515 6.8975275252923405 4 1.983533879574502 4.165698086774643 5 1.0989444398094785 2.88492094685572 6 0.6678808358568317 2.103964506559683 7 0.4501538738941292 1.6544254743786628 8 0.29856772133582393 1.2540678012599498 9 0.24707369355939637 1.1675008186602762 >10 1.378953368783325 13.598552082953773 >50 0.11657929868991573 4.137954998349551 >100 0.054682012054395136 4.956561657948528 >500 0.0030249623676497817 1.0421106438332417 >1k 9.307576515845482E-4 1.0490743374672973 >5k 4.653788257922741E-4 1.5274923071332966 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6971 0.846970600849765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5532 0.672133318591436 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3529 0.4287705136133727 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2646 0.32148676084471073 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1292 0.15697690665584513 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1120 0.13607905220940136 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 912 0.1108072282276554 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.2149915375839408E-4 0.0 0.0 0.0 0.0 6 1.2149915375839408E-4 0.0 0.0 0.0 0.0 7 1.2149915375839408E-4 0.0 0.0 0.0 0.0 8 1.2149915375839408E-4 0.0 0.0 0.0 0.0 9 1.2149915375839408E-4 0.0 0.0 0.0 0.0 10-11 1.2149915375839408E-4 0.0 0.0 6.074957687919704E-5 0.0 12-13 1.2149915375839408E-4 0.0 0.0 1.2149915375839408E-4 0.0 14-15 1.2149915375839408E-4 0.0 0.0 1.2149915375839408E-4 0.0 16-17 1.2149915375839408E-4 0.0 0.0 1.2149915375839408E-4 0.0 18-19 1.2149915375839408E-4 0.0 0.0 1.2149915375839408E-4 0.0 20-21 1.2149915375839408E-4 0.0 0.0 3.644974612751822E-4 0.0 22-23 1.2149915375839408E-4 0.0 0.0 6.074957687919704E-4 0.0 24-25 1.2149915375839408E-4 0.0 0.0 0.0012149915375839408 0.0 26-27 1.2149915375839408E-4 0.0 0.0 0.0018832368832551083 0.0 28-29 3.644974612751822E-4 0.0 0.0 0.003523475458993428 0.0 30-31 4.859966150335763E-4 0.0 0.0 0.006621703879832477 0.0 32-33 5.467461919127733E-4 0.0 0.0 0.01269666156775218 0.0 34-35 7.897444994295615E-4 0.0 0.0 0.017313629410571157 0.0 36-37 9.719932300671526E-4 0.0 0.0 0.0225380930221821 0.0 38-39 0.0010934923838255466 0.0 0.0 0.027641057480034655 0.0 40-41 0.0010934923838255466 0.0 0.0 0.03158977997718246 0.0 42-43 0.0011542419607047437 0.0 0.0 0.036996492319430994 0.0 44-45 0.0013364906913423348 0.0 0.0 0.041674209739129164 0.0 46-47 0.0013364906913423348 0.0 0.0 0.04981465304094157 0.0 48-49 0.0013364906913423348 0.0 0.0 0.059473835764733904 0.0 50-51 0.0013364906913423348 0.0 0.0 0.0676142790665463 0.0 52-53 0.001397240268221532 0.0 0.0 0.08480640932335906 0.0 54-55 0.0014579898451007288 0.0 0.0 0.12247114698846123 0.0 56-57 0.0014579898451007288 0.0 0.0 0.1943378964365513 0.0 58-59 0.0014579898451007288 0.0 0.0 0.2730693480719907 0.0 60-61 0.0014579898451007288 0.0 0.0 0.3413518724842082 0.0 62-63 0.0014579898451007288 0.0 0.0 0.4305930009197486 0.0 64-65 0.0014579898451007288 0.0 0.0 0.5424937215312295 0.0 66-67 0.0014579898451007288 0.0 0.0 0.7166627584438874 0.0 68-69 0.001579488998859123 0.0 0.0 0.9403427005130909 0.0 70-71 0.001579488998859123 0.0 0.0 1.122287683266286 0.0 72-73 0.001579488998859123 0.0 0.0 1.3181443191248174 0.0 74-75 0.001579488998859123 0.0 0.0 1.5245106317834496 0.0 76-77 0.001579488998859123 0.0 0.0 1.7651397058019491 0.0 78-79 0.001579488998859123 0.0 0.0 1.925397089609271 0.0 80-81 0.001579488998859123 0.0 0.0 2.056130179053303 0.0 82-83 0.001579488998859123 0.0 0.0 2.2385611584215317 0.0 84-85 0.001700988152617517 0.0 0.0 2.577786795714968 0.0 86-87 0.001822487306375911 0.0 0.0 3.053152234794685 6.074957687919704E-5 88-89 0.001822487306375911 0.0 0.0 3.6028751559745387 1.2149915375839408E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6030 0.0 28.761953 1 GTATCAA 8665 0.0 28.350765 1 GTACATG 4800 0.0 23.36219 1 ATCAACG 10380 0.0 23.246138 3 TATCAAC 10445 0.0 23.237902 2 TCAACGC 10440 0.0 23.155222 4 CAACGCA 10480 0.0 23.021526 5 GAGTACT 5605 0.0 22.920462 12-13 AACGCAG 10675 0.0 22.644108 6 ATGGGAG 1315 0.0 22.03108 5 TACATGG 5005 0.0 21.827711 2 AGTACTT 5795 0.0 21.59528 12-13 ACATGGG 4945 0.0 21.420174 3 GTACTTT 6035 0.0 21.209301 14-15 AGAGTAC 8590 0.0 21.00917 10-11 CATGGGA 3055 0.0 20.831726 4 CGATACA 115 0.0037149899 20.659271 1 CCGATCA 115 0.0037267809 20.647976 9 ACGCAGA 11720 0.0 20.462988 7 TCGCTTA 70 4.8496772E-4 20.359188 72-73 >>END_MODULE