FastQCFastQC Report
Thu 26 May 2016
SRR936933_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936933_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846269
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55630.6573559943705843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.4704177985959547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25070.2962415024064452No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19440.22971419253216174No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13120.15503344681182932No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11700.1382539121721344No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.10516750584034155No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8610.101740699470263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69550.030.3384021
GTACATG49750.027.8930911
GGTATCA47400.026.669211
TACATGG51850.026.580072
ACATGGG51000.025.9980563
TATCAAC86100.024.3964142
ATCAACG85750.024.385153
TCAACGC86000.024.3695224
CAACGCA86050.024.3553625
CATGGGA31800.024.214
AACGCAG87650.023.9107696
GAGTACT42300.023.02459312-13
AGTACTT45300.021.97173112-13
ACGCAGA96050.021.6191987
ATGGGCG1553.8900904E-521.462155
CGCAGAG95700.021.4500038
AGAGTAC71950.021.26197210-11
CATGGGG19700.021.2286974
GTACTTT46200.021.02954314-15
CTGTGCG1604.9797858E-520.785319
Produced by FastQC (version 0.11.5)