FastQCFastQC Report
Thu 26 May 2016
SRR936933_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936933_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846269
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70070.8279873184531159No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53570.6330138525693367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.4318957683667959No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26200.3095942306760616No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12390.14640734801818336No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11330.13388177990686176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8540.10091353931196817No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8480.10020454489057262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85650.030.5547471
GGTATCA57700.026.9992981
ATCAACG104600.025.0162963
TATCAAC104500.024.9962672
TCAACGC104950.024.9328694
CAACGCA105050.024.8742165
GAGTACT54550.024.63739612-13
AACGCAG106500.024.5801626
TAGACTG2351.7644197E-1024.2603025
AGTACTT56750.023.18019312-13
GTACTTT58300.023.05265614-15
ACGCAGA115750.022.5337837
AGAGTAC84950.022.45457810-11
CGCAGAG115800.022.4009728
TAAGGTG3007.2759576E-1222.1712195
GCAGAGT117650.021.6449039
GTACATG48450.021.272591
TACTTTT64150.020.46923414-15
CTGGTCG2801.5697879E-920.3613229
ACTTTTT66650.020.30829616-17
Produced by FastQC (version 0.11.5)