##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936933_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 846269 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.370080908080055 33.0 31.0 34.0 31.0 34.0 2 32.488519607831556 34.0 31.0 34.0 31.0 34.0 3 32.580516360637105 34.0 31.0 34.0 31.0 34.0 4 36.206463902139866 37.0 35.0 37.0 35.0 37.0 5 36.138928638529826 37.0 35.0 37.0 35.0 37.0 6 36.20045990104801 37.0 36.0 37.0 35.0 37.0 7 36.15579207084272 37.0 36.0 37.0 35.0 37.0 8 36.126345169207426 37.0 35.0 37.0 35.0 37.0 9 37.840178477529015 39.0 38.0 39.0 35.0 39.0 10-11 37.75704474581959 39.0 37.0 39.0 35.0 39.0 12-13 37.67361796308266 39.0 37.0 39.0 35.0 39.0 14-15 38.884363009870384 40.0 38.0 41.0 35.5 41.0 16-17 38.814159563921166 40.0 38.0 41.0 35.0 41.0 18-19 38.9924657526153 40.0 38.0 41.0 36.0 41.0 20-21 39.03373513622737 40.0 38.0 41.0 36.0 41.0 22-23 38.94802420979617 40.0 38.0 41.0 35.0 41.0 24-25 38.83400963523418 40.0 38.0 41.0 35.0 41.0 26-27 38.65976539374596 40.0 38.0 41.0 35.0 41.0 28-29 38.57039723775773 40.0 38.0 41.0 34.5 41.0 30-31 38.51571722466497 40.0 38.0 41.0 35.0 41.0 32-33 38.29935930537453 40.0 38.0 41.0 34.0 41.0 34-35 38.11854800305813 40.0 37.0 41.0 34.0 41.0 36-37 37.944301989083854 40.0 37.0 41.0 33.5 41.0 38-39 37.76339733583529 40.0 37.0 41.0 33.0 41.0 40-41 37.56125180055042 40.0 37.0 41.0 33.0 41.0 42-43 37.26128748660296 40.0 36.5 41.0 33.0 41.0 44-45 36.89311909097462 40.0 36.0 41.0 31.5 41.0 46-47 36.369890661243645 39.5 36.0 41.0 30.5 41.0 48-49 35.877593294803425 39.0 35.5 41.0 29.0 41.0 50-51 35.547506762034295 39.0 35.0 40.5 28.0 41.0 52-53 35.2990290321399 39.0 35.0 40.0 27.0 41.0 54-55 35.00588347204021 38.5 35.0 40.0 26.0 41.0 56-57 34.702554388734555 38.0 34.5 40.0 25.5 41.0 58-59 34.34973454067206 38.0 34.0 40.0 25.0 41.0 60-61 33.998004180703774 37.5 34.0 40.0 23.5 41.0 62-63 33.64113833780985 37.0 33.5 40.0 23.0 41.0 64-65 33.25436356524935 36.5 33.0 39.0 22.0 40.5 66-67 32.856123762066204 36.0 33.0 39.0 21.0 40.0 68-69 32.54151162337271 35.5 32.0 39.0 20.0 40.0 70-71 32.06981586233219 35.0 32.0 38.0 18.5 40.0 72-73 31.56848886110681 35.0 31.0 37.0 15.5 39.5 74-75 31.078519950512188 35.0 31.0 37.0 12.0 39.0 76-77 29.703363233203625 33.5 29.5 35.0 9.0 37.5 78-79 30.406598847411402 34.0 30.5 36.0 7.5 38.0 80-81 30.154600369386095 34.0 30.0 35.5 7.0 37.0 82-83 29.852118534413997 34.0 30.0 35.0 2.0 37.0 84-85 29.521442945446424 34.0 30.0 35.0 2.0 36.0 86-87 29.209249068558577 34.0 29.5 35.0 2.0 36.0 88-89 28.831181338321503 34.0 29.0 35.0 2.0 35.5 90-91 28.405776413882585 34.0 29.0 35.0 2.0 35.0 92-93 27.9574857403497 33.5 28.5 35.0 2.0 35.0 94-95 27.404786185007367 33.0 27.0 35.0 2.0 35.0 96-97 26.780621764474418 33.0 25.5 34.5 2.0 35.0 98-99 26.028997281006394 32.5 24.5 34.0 2.0 35.0 100-101 22.56941350799805 28.5 10.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1788825077983276 1101 2 0.19371471680661045 1101 3 0.15319629918795385 1101 4 0.16022979282536198 1101 5 0.18947502079324607 1101 6 0.2363456753248343 1101 7 0.23996559296268316 1101 8 0.2507053189220585 1101 9 0.24140009330831447 1101 10-11 0.25306428069745834 1101 12-13 0.2715512561872444 1101 14-15 0.28337095847121674 1101 16-17 0.3378802089511552 1101 18-19 0.09278364652310245 1101 20-21 0.21187266179874342 1101 22-23 0.2916115809643287 1101 24-25 0.3016373815894795 1101 26-27 0.27081754941818303 1101 28-29 0.31397654251849616 1101 30-31 0.5412094456457623 1101 32-33 0.4847335694001984 1101 34-35 0.6168081916462427 1101 36-37 0.6630215434254225 1101 38-39 0.6479957744765414 1101 40-41 0.5349332187052909 1101 42-43 0.5728749362505567 1101 44-45 0.8433487794595962 1101 46-47 0.9296598638443925 1101 48-49 1.0318972605126788 1101 50-51 0.8867871298472423 1101 52-53 0.8125466403024859 1101 54-55 0.9523167601552842 1101 56-57 0.9745185646389274 1101 58-59 1.0014083982839708 1101 60-61 1.0937174597217236 1101 62-63 0.9514771415028562 1101 64-65 0.9158310955494784 1101 66-67 0.6523865472201678 1101 68-69 0.527548703406616 1101 70-71 0.7032945104267974 1101 72-73 0.8821353448181881 1101 74-75 0.889695224628337 1101 76-77 0.750344638361458 1101 78-79 0.7316268728212201 1101 80-81 0.8400058156742745 1101 82-83 0.8389765380870777 1101 84-85 0.771396632919032 1101 86-87 0.2708658291986481 1101 88-89 0.5295326349223686 1101 90-91 0.5073697414644549 1101 92-93 0.5238639534324534 1101 94-95 0.5108157616728839 1101 96-97 0.7503264847705786 1101 98-99 0.670395029664359 1101 100-101 1.2313199058564024 1102 1 0.18770972663914876 1102 2 0.2524001524476347 1102 3 0.18784042777338783 1102 4 0.12272129025029699 1102 5 0.1532297579422135 1102 6 0.025786581323899327 1102 7 0.1784577440920927 1102 8 0.20214567722278076 1102 9 0.23765233489628912 1102 10-11 0.2933046495987952 1102 12-13 0.3134700554911305 1102 14-15 0.4314802949611689 1102 16-17 0.4282868749699986 1102 18-19 0.24927409272056877 1102 20-21 0.17415214696230663 1102 22-23 0.1879353766905183 1102 24-25 0.27252655217984767 1102 26-27 0.1613231544923437 1102 28-29 0.19339346463048912 1102 30-31 0.23919466662437117 1102 32-33 0.19715218761029263 1102 34-35 0.11874169930077016 1102 36-37 0.12832662023570407 1102 38-39 0.11214693038505885 1102 40-41 0.003395290595115341 1102 42-43 0.0697797780805729 1102 44-45 0.032418443155144416 1102 46-47 0.18079832551875796 1102 48-49 0.17028163874255142 1102 50-51 0.06915853115995674 1102 52-53 0.07594206083763311 1102 54-55 0.07639804035598985 1102 56-57 -0.12057997689338862 1102 58-59 0.07989710421413321 1102 60-61 -0.07010728564271318 1102 62-63 -0.0680544880763918 1102 64-65 -0.16012671401050937 1102 66-67 -0.18722286435747293 1102 68-69 -0.15736684504260268 1102 70-71 -0.27766192988732996 1102 72-73 -0.33393938067010254 1102 74-75 -0.13301548464101742 1102 76-77 -0.3216445666808987 1102 78-79 -0.3368133353041465 1102 80-81 -0.24304115387545977 1102 82-83 -0.3576438539589262 1102 84-85 -0.31009447581675786 1102 86-87 -0.36985399659793927 1102 88-89 -0.22502673292773778 1102 90-91 -0.11785773002430844 1102 92-93 0.105777982460463 1102 94-95 -0.1462897409286832 1102 96-97 -0.10309132955968536 1102 98-99 -0.09216338413057557 1102 100-101 0.2682078308747329 1103 1 0.30249845410772735 1103 2 0.26338870225283983 1103 3 0.09858090168228983 1103 4 0.162114996490196 1103 5 0.010491690182988123 1103 6 0.11687403507196592 1103 7 0.08595387956390965 1103 8 0.16326957294696598 1103 9 0.16669889808919436 1103 10-11 0.26820037145535025 1103 12-13 0.2863186848951145 1103 14-15 0.2971491789227443 1103 16-17 0.26955152578685926 1103 18-19 0.3035698392914554 1103 20-21 0.2770232057847366 1103 22-23 0.12202389595253749 1103 24-25 0.12325783981965088 1103 26-27 0.25370461315063153 1103 28-29 0.2906822329010268 1103 30-31 0.2820950095939594 1103 32-33 0.2852622642946514 1103 34-35 0.1270495605554629 1103 36-37 0.138293955354996 1103 38-39 0.024283996980841493 1103 40-41 0.02063761458131097 1103 42-43 0.13562655857303696 1103 44-45 0.32981982482596806 1103 46-47 0.14573195822188012 1103 48-49 0.272501614914205 1103 50-51 0.42431158512811606 1103 52-53 0.44459939667970616 1103 54-55 0.22458258644005014 1103 56-57 0.22531977619647847 1103 58-59 0.44214911575058835 1103 60-61 0.48681960424562476 1103 62-63 0.5686373125955626 1103 64-65 0.08286642426615742 1103 66-67 0.17564001075822944 1103 68-69 0.07754692293648446 1103 70-71 0.2681580845215912 1103 72-73 0.5706091048778461 1103 74-75 0.4903404879493962 1103 76-77 0.3210492018111317 1103 78-79 0.5268078733197505 1103 80-81 0.8320251201598055 1103 82-83 0.7013027327825334 1103 84-85 0.4674311070100501 1103 86-87 0.2998471698717111 1103 88-89 0.37600007745507824 1103 90-91 0.4925337122898803 1103 92-93 0.42021126659829733 1103 94-95 0.2976285525702842 1103 96-97 0.4172612692917994 1103 98-99 0.3300838244327089 1103 100-101 0.6170744690966075 1104 1 0.13419304037577717 1104 2 0.14560005536734622 1104 3 0.18639718321825427 1104 4 0.07026023429010309 1104 5 0.08740720279848802 1104 6 0.021261273294278737 1104 7 0.08205843307936789 1104 8 0.11424796025894324 1104 9 0.15713460478419705 1104 10-11 0.20404674827666014 1104 12-13 0.09565971078541935 1104 14-15 0.0709713539138832 1104 16-17 0.14154599215203234 1104 18-19 0.11999943798001311 1104 20-21 0.05120184877030454 1104 22-23 0.15093412077321489 1104 24-25 0.19383850271602654 1104 26-27 0.1959115411452217 1104 28-29 0.16679004938369957 1104 30-31 0.0627677395688977 1104 32-33 0.27783957259253356 1104 34-35 0.2513216786122996 1104 36-37 0.289633056988599 1104 38-39 0.38464799661878857 1104 40-41 0.36229486669356703 1104 42-43 0.5346606308790598 1104 44-45 0.29122518033853595 1104 46-47 0.43799157419745427 1104 48-49 0.4103049898503741 1104 50-51 0.6249561328998539 1104 52-53 0.6231591318876823 1104 54-55 0.4815362266040424 1104 56-57 0.41104239492882044 1104 58-59 0.6370348616123849 1104 60-61 0.597134715247968 1104 62-63 0.7552069618601038 1104 64-65 0.5910579246835255 1104 66-67 0.3649301030711527 1104 68-69 0.424758482780085 1104 70-71 0.3859919883154639 1104 72-73 0.5141011055796056 1104 74-75 0.5121654923354022 1104 76-77 0.507279703626434 1104 78-79 0.6148739059427193 1104 80-81 0.7711780449294139 1104 82-83 0.7792658590180181 1104 84-85 0.4983734228898591 1104 86-87 0.5035339875958798 1104 88-89 0.40809225404418115 1104 90-91 0.6359388867748201 1104 92-93 0.6424522993050736 1104 94-95 0.7035115036782962 1104 96-97 0.6156155940084389 1104 98-99 0.6573806684058816 1104 100-101 0.6805050991956918 1105 1 0.1312864277079555 1105 2 0.11206116764270746 1105 3 0.20286344158684955 1105 4 0.10669829454775481 1105 5 0.11661593206066101 1105 6 0.1722612894145712 1105 7 0.11155856762180605 1105 8 0.0465650538446738 1105 9 0.13285992492700416 1105 10-11 0.08586144675900442 1105 12-13 0.026277973588342718 1105 14-15 0.1958267362934123 1105 16-17 0.027591027288075054 1105 18-19 -0.10197698423275625 1105 20-21 0.11858652978072115 1105 22-23 0.08132669948673765 1105 24-25 -0.045920461716413286 1105 26-27 0.04039908325948005 1105 28-29 0.03936844050414834 1105 30-31 0.11589030214972951 1105 32-33 0.142722384269085 1105 34-35 0.3665321662263352 1105 36-37 0.19355053597332272 1105 38-39 0.17009930899656922 1105 40-41 0.011530330840180625 1105 42-43 0.1756685459424716 1105 44-45 0.014551367009040916 1105 46-47 -0.0631907872269366 1105 48-49 0.1398598093837009 1105 50-51 0.1311742255716979 1105 52-53 0.19936285269910314 1105 54-55 0.23818553708955648 1105 56-57 0.1566976662023407 1105 58-59 0.07545410576909006 1105 60-61 0.057742921468666 1105 62-63 0.14679737747844257 1105 64-65 0.05399489310138961 1105 66-67 0.23952167067035646 1105 68-69 0.03335221049842829 1105 70-71 -0.17129580973944059 1105 72-73 -0.022664940621147878 1105 74-75 0.21805448304391817 1105 76-77 0.25090133805045056 1105 78-79 0.2878105395545205 1105 80-81 0.14344085033144793 1105 82-83 0.13971001026603957 1105 84-85 0.05867633557863883 1105 86-87 0.016523222960824313 1105 88-89 0.12310409005864997 1105 90-91 0.03124564973336419 1105 92-93 0.13632658830718825 1105 94-95 -0.14251490632025465 1105 96-97 -0.14245478222677832 1105 98-99 -0.2876857161970072 1105 100-101 -0.05367314411407875 1106 1 0.009951573090795307 1106 2 0.04298471449772023 1106 3 -0.026090253737216074 1106 4 0.028829377225122244 1106 5 0.01824133654135096 1106 6 0.11366500466591845 1106 7 0.09419233580635478 1106 8 0.14377422701348763 1106 9 0.09394920635060089 1106 10-11 -0.03224331751633969 1106 12-13 0.11894343838324062 1106 14-15 0.1619251756346145 1106 16-17 0.1865121915018335 1106 18-19 0.024461988641675703 1106 20-21 0.11183139652690244 1106 22-23 0.05588740000628434 1106 24-25 0.06879378679479942 1106 26-27 0.21502898972354956 1106 28-29 0.32193300274418135 1106 30-31 0.3142559771522855 1106 32-33 0.2312714622185723 1106 34-35 0.26411600109894096 1106 36-37 0.24524996635516771 1106 38-39 0.29177515756072125 1106 40-41 0.39424136221018813 1106 42-43 0.3179630179557904 1106 44-45 0.25960299809676 1106 46-47 0.09607496791639392 1106 48-49 -0.11970348815725629 1106 50-51 -0.035255280044637516 1106 52-53 -0.07346971351775267 1106 54-55 -0.12362055101925051 1106 56-57 -0.051240298797587513 1106 58-59 0.1097506151133203 1106 60-61 -0.08865354945979931 1106 62-63 0.32926438955134785 1106 64-65 -0.021430953664271613 1106 66-67 0.2418640257155502 1106 68-69 0.28181953622822675 1106 70-71 0.05526517278353893 1106 72-73 0.05157534069166303 1106 74-75 0.024893641810411538 1106 76-77 0.26420657655197743 1106 78-79 0.10964623802378881 1106 80-81 0.15126140982050273 1106 82-83 0.2131229855893011 1106 84-85 0.03479740713413193 1106 86-87 0.10979801660193544 1106 88-89 0.11679210505662851 1106 90-91 -0.004729211674895595 1106 92-93 0.08519691996336931 1106 94-95 0.13252620719044472 1106 96-97 0.10791462788246875 1106 98-99 0.03083428374544539 1106 100-101 0.03524354545337616 1107 1 0.184840753667892 1107 2 0.14945091181153458 1107 3 0.1877569811911215 1107 4 0.057738919996481286 1107 5 0.137974927974561 1107 6 0.07209943448175693 1107 7 0.10819042362807352 1107 8 0.11993092545649375 1107 9 0.1750621000007868 1107 10-11 0.2198113902282799 1107 12-13 0.16603782603687023 1107 14-15 0.20216710006969407 1107 16-17 0.22024858490968313 1107 18-19 0.26888271500425276 1107 20-21 0.12197419116514396 1107 22-23 0.1226589565753855 1107 24-25 0.25449679221460286 1107 26-27 0.18372922410533477 1107 28-29 0.2798805489539262 1107 30-31 0.2734792909955388 1107 32-33 0.21342692025008603 1107 34-35 0.11105997781102417 1107 36-37 0.26395115829927107 1107 38-39 0.21504716271093827 1107 40-41 0.06806175593035135 1107 42-43 0.0368190403598021 1107 44-45 0.04747790215638048 1107 46-47 -0.0603781918182591 1107 48-49 0.4077129939945152 1107 50-51 0.28738723837371083 1107 52-53 -0.014920670859083884 1107 54-55 0.04924743649413443 1107 56-57 0.10192768562213672 1107 58-59 0.13392611713378244 1107 60-61 0.17307327421762153 1107 62-63 0.1488132451170685 1107 64-65 0.18701636641345232 1107 66-67 0.3052583336705439 1107 68-69 0.1756081978065538 1107 70-71 0.34659466702859376 1107 72-73 0.2871157814944283 1107 74-75 0.2833220316178249 1107 76-77 0.23487128684518055 1107 78-79 0.39767950815801 1107 80-81 0.3438161418724732 1107 82-83 0.1934671776792456 1107 84-85 0.22182847782137927 1107 86-87 0.11828201868205213 1107 88-89 0.14143091991858725 1107 90-91 0.3128021337590745 1107 92-93 0.2965176635620672 1107 94-95 0.591736999065013 1107 96-97 0.39471190604843187 1107 98-99 0.3534340801529616 1107 100-101 0.3280012807153909 1108 1 0.07590339064303464 1108 2 0.1052082197562072 1108 3 0.08637640566119131 1108 4 0.0497328019594363 1108 5 0.08444805518122678 1108 6 0.14653803167775692 1108 7 0.1464132766984747 1108 8 0.18379983996614868 1108 9 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60-61 -0.6593309933661899 2316 62-63 -0.54430522767273 2316 64-65 -0.7599214199948108 2316 66-67 -0.9265846300653031 2316 68-69 -0.933605332739905 2316 70-71 -0.6027615886271143 2316 72-73 -0.3865164630446962 2316 74-75 -0.6662226134532965 2316 76-77 -0.5682005877422114 2316 78-79 -0.5197745296551268 2316 80-81 -0.5626155049730528 2316 82-83 -0.65699510096319 2316 84-85 -0.5972057768489059 2316 86-87 -0.739050032959188 2316 88-89 -0.8644936856712668 2316 90-91 -0.5801950935351705 2316 92-93 -0.43768338819832664 2316 94-95 -0.5021566076100932 2316 96-97 -0.2005761043339227 2316 98-99 -0.6585761476211083 2316 100-101 -1.0765456668930327 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 15.0 6 29.0 7 77.0 8 185.0 9 376.0 10 713.0 11 1378.0 12 2224.0 13 3175.0 14 3995.0 15 5750.0 16 8308.0 17 12845.0 18 11356.0 19 5697.0 20 4479.0 21 4322.0 22 4392.0 23 4688.0 24 5258.0 25 6291.0 26 7397.0 27 9087.0 28 11256.0 29 14011.0 30 17844.0 31 22851.0 32 29792.0 33 40513.0 34 56980.0 35 84176.0 36 133331.0 37 195782.0 38 130003.0 39 7688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09925334234791 19.719113939461277 15.290347821667375 20.891284896523437 2 15.631322203104613 21.787966306793475 39.52629563803438 23.054415852067535 3 19.137458981621638 26.74037862754899 30.789573503397865 23.33258888743151 4 12.878456655472393 17.80255853360527 37.68767739694139 31.63130741398095 5 13.095142437147569 38.379213204327705 33.60980209578999 14.915842262734735 6 30.25740042468766 37.36400600754607 17.416093464371258 14.962500103395021 7 27.063380556300654 34.52625583591033 21.25565275343892 17.154710854350093 8 26.70403855039001 33.57112218455361 20.021647962999943 19.70319130205644 9 27.560385645698943 16.43720849989779 20.730760550132405 35.27164530427086 10-11 24.72253507976784 27.271217749245697 27.00424159982414 21.00200557116232 12-13 26.541678827890426 25.111105334119525 27.763689796034118 20.583526041955928 14-15 23.46753809958772 25.513577833998408 27.494271915903806 23.524612150510062 16-17 21.309181832254286 28.934830414442693 27.6709296925682 22.085058060734823 18-19 21.06123905559189 26.743498346559086 31.604443286415435 20.590819311433588 20-21 22.362787927630063 25.478781419015128 31.702024075257928 20.456406578096882 22-23 22.02669304183572 25.68057566878838 31.59643627980131 20.696295009574587 24-25 21.838986731428875 25.73689170943277 31.798421716612157 20.6256998425262 26-27 21.611252819142933 25.923470572490604 31.81730358469128 20.64797302367519 28-29 21.1888460602222 26.15779199447928 31.928318046571647 20.725043898726877 30-31 21.32926279492841 25.906609622407732 31.711026436244417 21.05310114641944 32-33 21.333510591154834 26.204555526012584 31.48772489586103 20.97420898697155 34-35 21.892034329509887 25.856908382559208 31.482542784859184 20.76851450307172 36-37 21.865477486910375 25.749377648281612 31.296270144075972 21.088874720732036 38-39 21.56570768784409 26.1107674016927 31.52020213659229 20.803322773870914 40-41 21.677203223595583 26.08554108666367 31.297709923664126 20.93954576607662 42-43 21.49925142005674 26.51804568051057 30.601794464880548 21.380908434552133 44-45 21.88458988808523 26.253886175672275 30.466376530394 21.395147405848494 46-47 22.336771180692597 26.723512465347778 29.702126754471998 21.237589599487634 48-49 22.354428031307922 27.074427511360554 29.073388798324824 21.4977556590067 50-51 22.018560580097894 27.364506347184243 29.060850718859086 21.55608235385877 52-53 22.4168083670795 27.671166024041998 28.414428509138347 21.497597099740155 54-55 21.992058943437698 27.46134348023894 28.542780669672137 22.003816906651224 56-57 22.26899817721489 27.61995078184778 28.435802217475693 21.675248823461637 58-59 22.763028689527065 27.029235454058824 28.365988511899907 21.8417473445142 60-61 22.297954806632774 27.339003429827386 29.058643844479043 21.3043979190608 62-63 22.028569504039744 27.18215304112302 29.285809668205676 21.50346778663156 64-65 22.014526122619476 27.46988692123126 28.82855736684043 21.687029589308832 66-67 22.17169225497118 27.500007385502556 28.429871699049635 21.898428660476633 68-69 22.058798332201892 27.364757277710126 28.80532173413663 21.771122655951352 70-71 22.23807624702737 27.387955864758272 28.601290970591275 21.772676917623077 72-73 22.21846717769409 27.361276379023693 28.626358758267173 21.793897685015047 74-75 22.067510448805287 28.060167629914368 28.10601593583128 21.766305985449073 76-77 22.173859612014617 27.687591061470997 28.27977865194164 21.858770674572742 78-79 22.177141327718015 27.690067964027453 28.014142284283782 22.118648423970747 80-81 22.16952593429396 27.92028161757581 27.88577661080479 22.024415837325435 82-83 22.206550326972724 27.623655488278676 28.099327382215073 22.070466802533527 84-85 21.95340861821207 27.865312074572685 27.825782786265314 22.355496520949934 86-87 22.010980349523006 27.771586986655073 27.83799848506155 22.379434178760373 88-89 22.007153762165178 27.743516238354754 27.359949803135645 22.88938019634442 90-91 21.984556837726267 27.53057702408644 27.71213964428379 22.7727264939035 92-93 22.07174445432443 27.660402886794067 27.64551397755438 22.622338681327122 94-95 21.923761829867335 27.382841626007803 27.743719786498144 22.94967675762671 96-97 21.88068315428874 27.644280479432226 27.703955445842897 22.771080920436134 98-99 21.724256710008632 28.068506578103868 27.129722796156475 23.077513915731025 100-101 21.385483085736436 28.304331488370117 27.079305846374908 23.230879579518547 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 509.0 1 392.0 2 275.5 3 302.0 4 298.5 5 300.0 6 458.5 7 961.0 8 1624.5 9 1972.5 10 1885.5 11 1702.5 12 1727.5 13 1828.0 14 1977.5 15 2069.0 16 2143.5 17 2282.0 18 2341.5 19 2489.5 20 2656.0 21 2781.0 22 3077.0 23 3492.0 24 3912.5 25 4504.5 26 5269.0 27 6305.5 28 7408.0 29 8383.0 30 9804.5 31 11532.0 32 13310.5 33 15150.5 34 16950.0 35 18439.0 36 20717.5 37 23187.0 38 24779.5 39 25301.5 40 25050.0 41 25503.0 42 25968.0 43 26891.0 44 28902.5 45 29884.0 46 29319.0 47 30701.0 48 35187.5 49 34888.0 50 30889.5 51 28993.5 52 27540.5 53 26826.5 54 25970.0 55 24068.0 56 21928.5 57 19828.5 58 18406.0 59 16856.0 60 14234.5 61 11441.0 62 8951.5 63 6757.0 64 4780.0 65 3429.5 66 2528.5 67 2006.0 68 1442.5 69 862.5 70 591.0 71 416.5 72 280.0 73 210.5 74 161.5 75 128.0 76 94.5 77 45.0 78 22.0 79 14.0 80 6.5 81 4.5 82 4.5 83 2.5 84 2.0 85 2.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027414450960628358 2 0.005317458160466707 3 2.3633147379852033E-4 4 3.5449721069778046E-4 5 1.1816573689926016E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.014888882849306782 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.1358007914741056E-4 20-21 7.089944213955609E-4 22-23 0.0021860661326363134 24-25 0.011402993610778605 26-27 0.0053765410289163375 28-29 3.5449721069778046E-4 30-31 0.003013226290931134 32-33 0.005199292423567447 34-35 0.0 36-37 0.009866839031088223 38-39 0.012820982453569727 40-41 0.0010634916320933416 42-43 0.0 44-45 0.0 46-47 3.5449721069778046E-4 48-49 0.003485889238528175 50-51 0.007917104372250431 52-53 0.0 54-55 0.004017635054574846 56-57 0.0043130493968229966 58-59 2.3633147379852033E-4 60-61 0.0019497346588377928 62-63 0.006144618318761529 64-65 5.908286844963008E-5 66-67 0.0019497346588377928 68-69 4.1358007914741056E-4 70-71 7.680772898451911E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001477071711240752 80-81 0.0017724860534889025 82-83 0.011521159347677865 84-85 0.00720810995085487 86-87 0.003544972106977805 88-89 1.1816573689926016E-4 90-91 5.317458160466708E-4 92-93 1.7724860534889023E-4 94-95 0.0 96-97 0.002126983264186683 98-99 0.0017134031850392724 100-101 1.1816573689926016E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 846269.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.04176597980037 #Duplication Level Percentage of deduplicated Percentage of total 1 76.19667591339878 39.65409576323791 2 13.05776045983395 13.59097828141938 3 4.34385432196634 6.781855502223502 4 1.9967134236165314 4.156499708823101 5 1.12475790585869 2.9267193860314147 6 0.646318133390295 2.0181322227839478 7 0.4553433890688497 1.6587811866059188 8 0.3201769291253236 1.3330058254136976 9 0.2646687875219801 1.239644799213876 >10 1.4183138686664531 13.961440634037611 >50 0.11312866233714096 4.017132345334021 >100 0.05816619163904244 5.18380006812736 >500 0.0027480090510813003 0.9739065712773424 >1k 9.160030170271001E-4 1.0305151886729293 >5k 4.5800150851355004E-4 1.4734925167979762 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7007 0.8279873184531159 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5357 0.6330138525693367 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3655 0.4318957683667959 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2620 0.3095942306760616 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1239 0.14640734801818336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1133 0.13388177990686176 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 854 0.10091353931196817 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 848 0.10020454489057262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 1.1816573689926016E-4 0.0 0.0 0.0 0.0 6 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 7 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 8 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 9 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 10-11 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 12-13 1.1816573689926016E-4 0.0 0.0 0.0 1.1816573689926016E-4 14-15 2.3633147379852033E-4 0.0 0.0 0.0 1.1816573689926016E-4 16-17 2.3633147379852033E-4 0.0 0.0 1.1816573689926016E-4 1.1816573689926016E-4 18-19 2.3633147379852033E-4 0.0 0.0 1.1816573689926016E-4 1.1816573689926016E-4 20-21 2.3633147379852033E-4 0.0 0.0 1.1816573689926016E-4 1.1816573689926016E-4 22-23 2.3633147379852033E-4 0.0 0.0 3.544972106977805E-4 1.1816573689926016E-4 24-25 2.3633147379852033E-4 0.0 0.0 0.0014179888427911218 1.1816573689926016E-4 26-27 2.3633147379852033E-4 0.0 0.0 0.002954143422481504 1.1816573689926016E-4 28-29 4.135800791474106E-4 0.0 0.0 0.005258375292017077 1.1816573689926016E-4 30-31 4.7266294759704065E-4 0.0 0.0 0.009866839031088225 1.1816573689926016E-4 32-33 4.7266294759704065E-4 0.0 0.0 0.01494796571775641 1.1816573689926016E-4 34-35 4.7266294759704065E-4 0.0 0.0 0.019674595193726815 1.1816573689926016E-4 36-37 7.08994421395561E-4 0.0 0.0 0.025110219091092782 1.1816573689926016E-4 38-39 8.271601582948211E-4 0.0 0.0 0.029246019882566893 1.1816573689926016E-4 40-41 8.271601582948211E-4 0.0 0.0 0.0326137433841958 1.1816573689926016E-4 42-43 0.0010044087636437115 0.0 0.0 0.03799028441311214 1.1816573689926016E-4 44-45 0.0011816573689926017 0.0 0.0 0.04248058241528403 1.1816573689926016E-4 46-47 0.0014179888427911218 0.0 0.0 0.05116576407737965 1.1816573689926016E-4 48-49 0.0015361545796903821 0.0 0.0 0.05955553139722712 1.1816573689926016E-4 50-51 0.0015361545796903821 0.0 0.0 0.06640914413738422 1.1816573689926016E-4 52-53 0.0015952374481400123 0.0 0.0 0.08129802698669099 2.3633147379852033E-4 54-55 0.0017724860534889025 0.0 0.0 0.11733857674096534 2.3633147379852033E-4 56-57 0.0017724860534889025 0.0 0.0 0.18670186430083105 2.3633147379852033E-4 58-59 0.0017724860534889025 0.0 0.0 0.26079178133666714 2.3633147379852033E-4 60-61 0.0017724860534889025 0.0 0.0 0.3282053342376951 2.3633147379852033E-4 62-63 0.0017724860534889025 0.0 0.0 0.41960653172927287 2.3633147379852033E-4 64-65 0.0017724860534889025 0.0 0.0 0.529146169834887 2.3633147379852033E-4 66-67 0.0017724860534889025 0.0 0.0 0.7004864883388142 2.3633147379852033E-4 68-69 0.0017724860534889025 0.0 0.0 0.9160798753115145 2.3633147379852033E-4 70-71 0.0019497346588377928 0.0 0.0 1.0904334201063728 3.544972106977805E-4 72-73 0.002008817527287423 0.0 0.0 1.2727631521419314 3.544972106977805E-4 74-75 0.002008817527287423 0.0 0.0 1.465727800498423 3.544972106977805E-4 76-77 0.002008817527287423 0.0 0.0 1.7030046001921375 3.544972106977805E-4 78-79 0.002008817527287423 0.0 0.0 1.8643008310596274 3.544972106977805E-4 80-81 0.0021269832641866828 0.0 0.0 1.9971191193343962 3.544972106977805E-4 82-83 0.002186066132636313 0.0 0.0 2.1775581995795665 3.544972106977805E-4 84-85 0.002245149001085943 0.0 0.0 2.498437258129507 3.544972106977805E-4 86-87 0.002245149001085943 0.0 0.0 2.943744837634369 4.7266294759704065E-4 88-89 0.002245149001085943 0.0 0.0 3.470586775599721 5.908286844963008E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8565 0.0 30.554747 1 GGTATCA 5770 0.0 26.999298 1 ATCAACG 10460 0.0 25.016296 3 TATCAAC 10450 0.0 24.996267 2 TCAACGC 10495 0.0 24.932869 4 CAACGCA 10505 0.0 24.874216 5 GAGTACT 5455 0.0 24.637396 12-13 AACGCAG 10650 0.0 24.580162 6 TAGACTG 235 1.7644197E-10 24.260302 5 AGTACTT 5675 0.0 23.180193 12-13 GTACTTT 5830 0.0 23.052656 14-15 ACGCAGA 11575 0.0 22.533783 7 AGAGTAC 8495 0.0 22.454578 10-11 CGCAGAG 11580 0.0 22.400972 8 TAAGGTG 300 7.2759576E-12 22.171219 5 GCAGAGT 11765 0.0 21.644903 9 GTACATG 4845 0.0 21.27259 1 TACTTTT 6415 0.0 20.469234 14-15 CTGGTCG 280 1.5697879E-9 20.361322 9 ACTTTTT 6665 0.0 20.308296 16-17 >>END_MODULE