FastQCFastQC Report
Thu 26 May 2016
SRR936932_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936932_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864371
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54300.6282024732435494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40800.4720195379067553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25860.2991770894673699No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21490.24862009484353365No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14910.1724953752497481No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10960.12679740528083427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9040.1045847211440458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGTA650.00581713829.2575995
GGTATCA50750.029.1334061
GTATCAA72600.028.6161841
GTACATG52400.028.5789451
TACATGG54800.026.6379152
ACATGGG55000.024.7255383
CATGGGG18450.024.2269674
ATCAACG87550.023.5165793
GAGTACT41400.023.4772212-13
TATCAAC88550.023.4657942
TCAACGC87850.023.38354
CAACGCA87850.023.3253155
AACGCAG89250.022.955446
ATGGGGA9800.022.3163855
CCCTATA2154.370304E-822.1158662
GTACTTT45650.021.60196714-15
CATGGGA35800.021.5178834
GTATAGG2457.4378477E-921.3448721
CGCAGAG96600.021.0587648
ACGCAGA96950.021.0317677
Produced by FastQC (version 0.11.5)