##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936932_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 864371 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83953880914561 33.0 31.0 34.0 30.0 34.0 2 31.935187552567125 33.0 31.0 34.0 30.0 34.0 3 31.945720066961986 33.0 31.0 34.0 30.0 34.0 4 35.559173086556584 37.0 35.0 37.0 33.0 37.0 5 35.559064336957164 37.0 35.0 37.0 33.0 37.0 6 35.641614538201765 37.0 35.0 37.0 33.0 37.0 7 35.59112811512649 37.0 35.0 37.0 33.0 37.0 8 35.5576829856624 37.0 35.0 37.0 33.0 37.0 9 37.183245388843446 39.0 37.0 39.0 34.0 39.0 10-11 37.05720923075855 39.0 37.0 39.0 33.0 39.0 12-13 36.978622026884295 39.0 37.0 39.0 33.0 39.0 14-15 38.104971707750494 40.0 38.0 41.0 33.0 41.0 16-17 38.052799087428895 40.0 38.0 41.0 33.0 41.0 18-19 38.037364742685725 40.0 38.0 41.0 33.0 41.0 20-21 37.98254511083782 40.0 38.0 41.0 33.0 41.0 22-23 37.870690941736825 40.0 38.0 41.0 33.0 41.0 24-25 37.826492906402464 40.0 37.5 41.0 33.0 41.0 26-27 37.84193824179664 40.0 38.0 41.0 33.0 41.0 28-29 37.762692177317376 40.0 37.0 41.0 33.0 41.0 30-31 37.59324641849391 40.0 37.0 41.0 33.0 41.0 32-33 37.36852289121222 40.0 36.5 41.0 32.0 41.0 34-35 37.17872071136121 39.5 36.0 41.0 31.5 41.0 36-37 36.98422378816504 39.0 36.0 41.0 31.0 41.0 38-39 36.8145055768877 39.0 36.0 41.0 31.0 41.0 40-41 36.61461571477988 39.0 35.0 41.0 30.0 41.0 42-43 36.360333120847415 39.0 35.0 41.0 30.0 41.0 44-45 36.05138245035985 39.0 35.0 40.0 29.5 41.0 46-47 35.62155717857263 39.0 35.0 40.0 27.5 41.0 48-49 35.314237752076366 38.5 35.0 40.0 26.5 41.0 50-51 34.6007547684964 38.0 34.0 39.5 25.0 40.5 52-53 34.78431772930836 38.0 34.5 40.0 26.0 40.5 54-55 35.15794722405079 39.0 35.0 40.0 26.0 41.0 56-57 35.021044782853664 39.0 35.0 40.5 26.0 41.0 58-59 34.80164593675632 38.0 34.5 40.0 26.0 41.0 60-61 34.49549036235598 38.0 34.0 40.0 25.0 41.0 62-63 34.187306145162204 37.5 34.0 40.0 24.0 41.0 64-65 33.847409850631266 37.0 34.0 40.0 23.0 41.0 66-67 33.46746420229276 36.5 33.0 39.5 22.0 41.0 68-69 33.07008506763878 36.0 33.0 39.0 21.5 41.0 70-71 32.64559951687412 35.5 32.5 39.0 20.0 40.5 72-73 32.24936225301404 35.0 32.0 38.0 19.0 40.0 74-75 31.85390532537533 35.0 32.0 37.0 18.0 39.5 76-77 31.409815923949324 35.0 31.5 37.0 14.5 39.0 78-79 30.949079735437678 35.0 31.0 36.0 9.5 39.0 80-81 30.52684842503971 35.0 31.0 36.0 7.0 37.5 82-83 30.13307191009416 34.0 30.5 35.0 4.5 37.0 84-85 29.766697401925793 34.0 30.0 35.0 2.0 37.0 86-87 29.43214198532806 34.0 30.0 35.0 2.0 36.0 88-89 29.05708659823155 34.0 29.0 35.0 2.0 36.0 90-91 28.693023597506162 34.0 29.0 35.0 2.0 35.5 92-93 28.3049367690494 34.0 29.0 35.0 2.0 35.0 94-95 27.94064122928696 34.0 29.0 35.0 2.0 35.0 96-97 27.446318189758795 34.0 27.0 35.0 2.0 35.0 98-99 26.865514923568696 34.0 26.5 35.0 2.0 35.0 100-101 24.704359586335034 31.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2534011101091451 1101 2 0.3322327509056855 1101 3 0.3168494593497364 1101 4 0.3563136247741099 1101 5 0.30857750822357133 1101 6 0.16302379379332876 1101 7 0.25449541087277794 1101 8 0.3696236966289348 1101 9 0.35682141508324605 1101 10-11 0.260316121530046 1101 12-13 0.2794915719677462 1101 14-15 0.573012490241382 1101 16-17 0.6014651402034445 1101 18-19 0.41169817567580225 1101 20-21 0.4530963814559854 1101 22-23 0.39993160522337945 1101 24-25 0.4999451171822713 1101 26-27 0.42536169574231764 1101 28-29 0.5791193465815496 1101 30-31 0.39139139938016854 1101 32-33 0.4732203812520339 1101 34-35 0.5699480613303152 1101 36-37 0.46385688896472743 1101 38-39 0.3219104536960131 1101 40-41 0.44841469302678405 1101 42-43 0.5740235746397886 1101 44-45 0.5950883146952819 1101 46-47 0.7648460173387619 1101 48-49 0.8948068509075284 1101 50-51 0.9821975568585586 1101 52-53 0.8485516826190391 1101 54-55 0.9022865075024455 1101 56-57 1.029210311024947 1101 58-59 0.9230308609200577 1101 60-61 1.0405586339679402 1101 62-63 0.9956085139699624 1101 64-65 0.9568053182455145 1101 66-67 0.9667049715189506 1101 68-69 0.8719137346450694 1101 70-71 0.8171451609040119 1101 72-73 0.6785222857674356 1101 74-75 0.6738761157365971 1101 76-77 0.6440870914528389 1101 78-79 0.8229092188704534 1101 80-81 0.9595447786732691 1101 82-83 0.8229918387955486 1101 84-85 0.7889471895600124 1101 86-87 0.7907819127471818 1101 88-89 0.9454836702369924 1101 90-91 0.8791845047768021 1101 92-93 0.9037760444015568 1101 94-95 1.0544838765424345 1101 96-97 1.0565282073813087 1101 98-99 0.9249748759893848 1101 100-101 1.1792090459763642 1102 1 -0.028405450378723884 1102 2 -0.020114626130432356 1102 3 -0.009020214508986157 1102 4 -0.05046849141417198 1102 5 0.0668704631953645 1102 6 0.0239538360185918 1102 7 -0.02709205626322131 1102 8 -0.10626335984282775 1102 9 0.04254377129644382 1102 10-11 0.20409423247457426 1102 12-13 -0.07447637159670961 1102 14-15 -0.18815312260436912 1102 16-17 0.03775424202934374 1102 18-19 -0.1115329623783623 1102 20-21 -0.08571120750315941 1102 22-23 -0.08638501907316964 1102 24-25 -0.13409746297692493 1102 26-27 -0.09064841514426547 1102 28-29 -0.1489486846202368 1102 30-31 -0.10487767622696254 1102 32-33 -0.357683912977663 1102 34-35 -0.3176081605587413 1102 36-37 -0.08203954687532189 1102 38-39 0.10191990549185448 1102 40-41 -0.09646504101923625 1102 42-43 -0.005828709363136397 1102 44-45 -0.11715972795006024 1102 46-47 -0.1079757239644934 1102 48-49 -0.004106011501846751 1102 50-51 -0.12775561872756924 1102 52-53 -0.001918915415274114 1102 54-55 -0.2275407889942329 1102 56-57 0.04552914436190747 1102 58-59 -0.3642258730318062 1102 60-61 -0.07902378496751794 1102 62-63 -0.39378917848816286 1102 64-65 -0.11441179059136886 1102 66-67 -0.3440092365943812 1102 68-69 -0.22755555245051085 1102 70-71 -0.5616183558883208 1102 72-73 -0.24375027360505896 1102 74-75 -0.27788803841821164 1102 76-77 -0.4602013440395609 1102 78-79 -0.5104739084064853 1102 80-81 -0.2676876196203395 1102 82-83 -0.3375646583182146 1102 84-85 -0.41849800088824196 1102 86-87 -0.18952684591696922 1102 88-89 -0.07576566886157465 1102 90-91 -0.3570723667874134 1102 92-93 -0.26251490432698077 1102 94-95 -0.38041280555130186 1102 96-97 -0.49149669321187517 1102 98-99 -0.3950933507668033 1102 100-101 -0.43031952307244126 1103 1 0.17930792197395462 1103 2 0.1962370650996128 1103 3 0.1971711683272055 1103 4 0.1675579759247583 1103 5 0.07096631915286622 1103 6 0.3488897831779383 1103 7 0.3557088745597099 1103 8 0.325211853470762 1103 9 0.26111086811612694 1103 10-11 0.3045279113406636 1103 12-13 0.27069792264676096 1103 14-15 0.42339578085800866 1103 16-17 0.4875932284666007 1103 18-19 0.46178109633870434 1103 20-21 0.4370863150034836 1103 22-23 0.5066972741360516 1103 24-25 0.33516011171221294 1103 26-27 0.41760793725213574 1103 28-29 0.511909153331942 1103 30-31 0.33176583739455623 1103 32-33 0.353141017093094 1103 34-35 0.3301952383552944 1103 36-37 0.1789992844247763 1103 38-39 0.3102532388251902 1103 40-41 0.4738050386648496 1103 42-43 0.553408415428251 1103 44-45 0.3100345225729413 1103 46-47 0.5224364472505059 1103 48-49 0.7636936265114613 1103 50-51 0.6348933482000163 1103 52-53 0.6148893726697224 1103 54-55 0.6551220462548102 1103 56-57 0.7432731915612081 1103 58-59 0.837675732402694 1103 60-61 0.7328224647092796 1103 62-63 0.9765831506846752 1103 64-65 0.811095931007948 1103 66-67 0.6990413240148854 1103 68-69 0.883623137345694 1103 70-71 0.9652368259277253 1103 72-73 0.7592114357243247 1103 74-75 0.9477336854846641 1103 76-77 0.9752169386391429 1103 78-79 1.0631839201434516 1103 80-81 0.8189188726345584 1103 82-83 0.8641342110610033 1103 84-85 0.7062111169454788 1103 86-87 0.8989189618539974 1103 88-89 0.8041058728726718 1103 90-91 0.8956777918755847 1103 92-93 0.9853842800276738 1103 94-95 1.0443633033267758 1103 96-97 0.9737032221678596 1103 98-99 1.1447310621800995 1103 100-101 1.4106707691025306 1104 1 0.1621691769327107 1104 2 0.03935127937264227 1104 3 0.1463697964988775 1104 4 0.37278795756994754 1104 5 0.21084088704060377 1104 6 0.28947577067116015 1104 7 0.4181630444714912 1104 8 0.2984994591930885 1104 9 0.19511781834864195 1104 10-11 0.4278865679326813 1104 12-13 0.34385640707705534 1104 14-15 0.317834000760449 1104 16-17 0.5126750963757374 1104 18-19 0.4851527333780652 1104 20-21 0.5663305796952969 1104 22-23 0.385269743745873 1104 24-25 0.22230650838557153 1104 26-27 0.30769315275033193 1104 28-29 0.47058578533854245 1104 30-31 0.6239887276164069 1104 32-33 0.1554305858234244 1104 34-35 0.3907091729793919 1104 36-37 0.3567505699445235 1104 38-39 0.39729852464553517 1104 40-41 0.2732070271782874 1104 42-43 0.6376947868694458 1104 44-45 0.5153943086916044 1104 46-47 0.6071031230262562 1104 48-49 0.5819366204857985 1104 50-51 0.5943055388896212 1104 52-53 0.5923785442439069 1104 54-55 0.7475496631813101 1104 56-57 0.8651869194090551 1104 58-59 0.5745290442385809 1104 60-61 0.8173604746472947 1104 62-63 0.4530543819142636 1104 64-65 0.49298843468574916 1104 66-67 0.413753864037929 1104 68-69 0.5082206296143568 1104 70-71 0.6419417052095611 1104 72-73 0.5831191424664155 1104 74-75 0.4830803545196929 1104 76-77 0.6047663577071916 1104 78-79 0.7655342920218828 1104 80-81 0.6565778831145614 1104 82-83 0.620290814465065 1104 84-85 0.4077314233751288 1104 86-87 0.5959223003339638 1104 88-89 0.5913244590514566 1104 90-91 0.647849170216908 1104 92-93 0.23799175585443422 1104 94-95 0.6844439642820461 1104 96-97 0.6751187741101425 1104 98-99 0.5317043862221631 1104 100-101 0.28081164111532075 1105 1 0.054341580613488816 1105 2 -0.0303237860818939 1105 3 0.1316514425450528 1105 4 0.080147398742767 1105 5 0.12000240849199173 1105 6 0.020705312000650622 1105 7 -0.006148791240924822 1105 8 0.011327755631462821 1105 9 -0.031228061648299388 1105 10-11 -0.0268441239699726 1105 12-13 0.06943017453711064 1105 14-15 0.3454041645883379 1105 16-17 0.2961738163465739 1105 18-19 0.1241230826714741 1105 20-21 0.1912126822720026 1105 22-23 0.21995113669236588 1105 24-25 0.20891838082121694 1105 26-27 0.18725548581154072 1105 28-29 0.17976751777678146 1105 30-31 0.13638744151073467 1105 32-33 0.17189197227069997 1105 34-35 -0.1187800834940731 1105 36-37 -0.14912514701712354 1105 38-39 0.32300531474452043 1105 40-41 0.25296433681853614 1105 42-43 0.3698087239961012 1105 44-45 0.06308563276223111 1105 46-47 0.12740591795475353 1105 48-49 0.20272236553146428 1105 50-51 0.13407098576379184 1105 52-53 0.423104263979603 1105 54-55 0.4045289264446055 1105 56-57 0.3137743959946917 1105 58-59 0.17191693931053464 1105 60-61 0.33618905641724695 1105 62-63 0.4517849205833002 1105 64-65 0.2389700149471352 1105 66-67 0.11384857827042794 1105 68-69 0.054181470497091766 1105 70-71 -0.053491932741650317 1105 72-73 0.08427566004733222 1105 74-75 0.46764140623075434 1105 76-77 0.19297014257774947 1105 78-79 0.21981849284247446 1105 80-81 -0.11269586875792825 1105 82-83 0.11671228091086405 1105 84-85 0.26686216600340273 1105 86-87 0.2511611730628651 1105 88-89 0.27740820386991416 1105 90-91 -0.061310105569269524 1105 92-93 0.16066755841555036 1105 94-95 0.30377134792356486 1105 96-97 -0.08342003758950867 1105 98-99 0.3592221071625943 1105 100-101 0.4456229920282482 1106 1 0.3153127840414811 1106 2 0.15957592825224154 1106 3 0.2411410568961294 1106 4 0.3028820442023701 1106 5 0.07791511093951442 1106 6 0.2798426858113814 1106 7 0.3255791846469336 1106 8 0.2666401118906947 1106 9 0.3184644844092759 1106 10-11 0.4094953584422072 1106 12-13 0.49362658797792136 1106 14-15 0.6333267624531018 1106 16-17 0.5909982407782692 1106 18-19 0.6064497766942836 1106 20-21 0.5051969948582453 1106 22-23 0.20097130276591457 1106 24-25 0.2890591559925042 1106 26-27 0.28507486267260873 1106 28-29 0.31096804069056816 1106 30-31 0.224140425266512 1106 32-33 0.39029775095227137 1106 34-35 0.4399569344447727 1106 36-37 0.19445539017996083 1106 38-39 0.07832296671634253 1106 40-41 0.2709258808667627 1106 42-43 -0.00954283869302941 1106 44-45 0.03433520910522958 1106 46-47 0.18850709330033766 1106 48-49 0.16654357016989962 1106 50-51 0.3467415077092184 1106 52-53 0.34780301358620846 1106 54-55 0.45040265849970496 1106 56-57 0.39838853524469897 1106 58-59 0.4079513886774677 1106 60-61 0.47393432356324894 1106 62-63 0.38176500870395813 1106 64-65 0.2739444139594198 1106 66-67 0.27486245001129817 1106 68-69 0.3111642458136217 1106 70-71 0.38095924986662766 1106 72-73 0.33890865203416354 1106 74-75 0.44420712148482977 1106 76-77 0.4981930769472278 1106 78-79 0.8055765060121551 1106 80-81 0.5799719902006011 1106 82-83 0.6301028684626111 1106 84-85 0.5696558813543113 1106 86-87 0.6246738343551392 1106 88-89 0.6059701001586788 1106 90-91 0.6776816210145995 1106 92-93 0.854074547545725 1106 94-95 0.9445344147161165 1106 96-97 0.9369207944728544 1106 98-99 0.9836085610323977 1106 100-101 1.495507848105909 1107 1 0.3923425089749344 1107 2 0.4822800098848923 1107 3 0.3769274366654294 1107 4 0.33640577978356134 1107 5 0.3889177728738531 1107 6 0.5307577257404006 1107 7 0.4331934259956469 1107 8 0.47152587054198136 1107 9 0.4309589387127346 1107 10-11 0.5313759041387485 1107 12-13 0.5546569783949664 1107 14-15 0.5674300233226646 1107 16-17 0.6152883916212133 1107 18-19 0.7094888183979151 1107 20-21 0.6639009655769641 1107 22-23 0.7661886940702587 1107 24-25 0.9359092003580685 1107 26-27 0.8751785671978993 1107 28-29 0.9074215677535733 1107 30-31 0.8480312859586121 1107 32-33 0.8479840863560071 1107 34-35 0.73978501785205 1107 36-37 0.6838769740308948 1107 38-39 0.7235231663614172 1107 40-41 0.9054090219492821 1107 42-43 1.0347395481747625 1107 44-45 0.6446807059996331 1107 46-47 0.7611834464502962 1107 48-49 0.6751116713234069 1107 50-51 0.5668502033613905 1107 52-53 0.7106440739233904 1107 54-55 0.6204753071243729 1107 56-57 0.7769655095126637 1107 58-59 0.5281820896535123 1107 60-61 0.7288561292420397 1107 62-63 0.7819979279586207 1107 64-65 0.7209830120073306 1107 66-67 0.5585941492127091 1107 68-69 0.5778151996734238 1107 70-71 0.527240860336903 1107 72-73 0.44807568841392964 1107 74-75 0.5376521081751591 1107 76-77 0.4563507965568121 1107 78-79 0.41231730015590173 1107 80-81 0.48970191477912905 1107 82-83 0.2207056856764531 1107 84-85 0.20583045988137272 1107 86-87 0.3632358352424525 1107 88-89 0.4702946343201688 1107 90-91 0.6808149395594114 1107 92-93 0.9255447116983397 1107 94-95 1.0106807101908295 1107 96-97 0.9557484342244074 1107 98-99 1.0731022389383469 1107 100-101 0.9618177416285398 1108 1 0.04890708383036113 1108 2 0.1676248374710987 1108 3 0.011505237050950967 1108 4 0.07979970781954648 1108 5 0.20001222564746968 1108 6 0.015196310019689463 1108 7 0.09770268922117964 1108 8 0.01729985854797178 1108 9 -0.15901616302211607 1108 10-11 -0.1949205458505432 1108 12-13 0.017135560532466343 1108 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-4.142523427035972 2316 98-99 -4.348412477720181 2316 100-101 -5.0558178226118144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1440.0 3 546.0 4 516.0 5 546.0 6 613.0 7 826.0 8 967.0 9 1216.0 10 1540.0 11 2088.0 12 2887.0 13 3768.0 14 4303.0 15 5653.0 16 7438.0 17 10250.0 18 8899.0 19 5023.0 20 4772.0 21 4806.0 22 5244.0 23 5754.0 24 6587.0 25 7531.0 26 8656.0 27 10475.0 28 12271.0 29 15110.0 30 18719.0 31 23448.0 32 30300.0 33 39768.0 34 55855.0 35 80741.0 36 122671.0 37 175901.0 38 151875.0 39 25362.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16630692293537 19.257650967951754 14.838994908806411 21.737047200306467 2 15.132024334555 20.917440960235893 40.3958473408238 23.554687364385313 3 18.69588008826977 26.44170295393609 30.5302550774212 24.332161880372936 4 12.291744312828573 17.09901592894132 38.425879841937494 32.183359916292616 5 13.097734765746685 37.99990967319066 34.13461705485964 14.76773850620302 6 29.92581610966012 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21.405511093628434 28-29 21.185043945688186 26.46587346719418 30.902383474361784 21.446699112755848 30-31 21.320484682160174 26.09638339548245 30.89073594355788 21.692395978799496 32-33 21.257952157184434 26.35410116399276 30.64295833841928 21.744988340403523 34-35 21.837515192781282 26.203375299444588 30.422528530032967 21.536580977741167 36-37 21.699264735657717 25.976351214545623 30.53950238049785 21.78488166929881 38-39 21.528913046244906 26.338286285494135 30.541527397426687 21.591273270834275 40-41 21.450599875391138 26.250412904248442 30.617218815822433 21.681768404537983 42-43 21.346043602029525 26.468041240691605 29.961629704960025 22.22428545231885 44-45 21.56127635721851 26.449744634844315 29.825805619363905 22.16317338857327 46-47 22.008228110241866 26.66193480911756 29.379282099695263 21.95055498094531 48-49 21.93387426774576 27.028634199418423 28.85551706245658 22.18197447037924 50-51 21.672219018139096 27.190247915048154 29.014743474420328 22.12278959239242 52-53 22.03371789450301 27.486337501742703 28.310339302004987 22.1696053017493 54-55 21.606980250316163 27.252631420614232 28.563395339634628 22.576992989434977 56-57 21.92404659068163 27.441157107601242 28.26281133828459 22.37198496343254 58-59 22.26366108601447 26.97739451167413 28.42279904966358 22.336145352647815 60-61 21.964827722247644 27.085638786804584 29.02843828075694 21.921095210190835 62-63 21.696859044002544 27.14722240541669 29.132954062925563 22.0229644876552 64-65 21.684185682478034 27.378892608462003 28.71493087552272 22.221990833537244 66-67 21.70814037182843 27.51035106843393 28.23347937076613 22.548029188971512 68-69 21.580492231337615 27.268155428804235 28.746481416322524 22.404870923535622 70-71 21.760726868205364 27.248297053782945 28.558041975041398 22.432934102970297 72-73 21.668976703719814 27.253385226252508 28.55032255675981 22.527315513267865 74-75 21.38447885052178 27.942627911039942 28.219687984884057 22.453205253554227 76-77 21.660873107160793 27.41790464464497 28.428518198797008 22.49270404939723 78-79 21.649998119544872 27.502379498988894 28.30935511961141 22.538267261854823 80-81 21.5908814624856 27.68389610239252 28.12546672301111 22.599755712110774 82-83 21.604776116639453 27.478061826130933 28.378328336031604 22.53883372119801 84-85 21.463951950589916 27.579699563912957 28.125184402685715 22.831164082811416 86-87 21.500615959870313 27.565086619175936 28.14083116681721 22.793466254136536 88-89 21.588210102319554 27.34860410204176 27.78832353349692 23.274862262141767 90-91 21.532895292702886 27.06721882779957 28.208539593217118 23.191346286280424 92-93 21.619485117528527 27.20540322197955 28.243362580916965 22.931749079574956 94-95 21.41477369695322 26.875724959431196 28.34135067354336 23.36815067007223 96-97 21.238652359377774 27.136561633794116 28.370215274672063 23.25457073215605 98-99 21.050813305703294 27.4298914801533 27.990400857372176 23.52889435677122 100-101 20.894372385591215 27.30782901462622 28.297035044800246 23.500763554982328 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 278.0 1 214.5 2 198.5 3 241.0 4 231.0 5 250.5 6 388.0 7 787.5 8 1327.0 9 1645.0 10 1575.0 11 1388.0 12 1389.5 13 1509.0 14 1666.0 15 1761.5 16 1806.5 17 1891.5 18 1963.0 19 2049.0 20 2221.0 21 2446.5 22 2773.0 23 3220.0 24 3651.0 25 4315.5 26 5195.5 27 6257.5 28 7326.5 29 8472.5 30 9947.0 31 11833.0 32 13840.0 33 15727.0 34 17680.5 35 19276.5 36 21980.5 37 24578.0 38 25802.0 39 26245.5 40 25898.0 41 26551.0 42 27054.5 43 27992.0 44 29980.0 45 31188.0 46 30962.0 47 31800.5 48 36093.0 49 36523.5 50 32406.0 51 29657.5 52 28184.5 53 27678.5 54 26853.0 55 24606.5 56 22182.0 57 20156.5 58 18525.5 59 16700.5 60 14246.0 61 11564.0 62 8914.0 63 6606.5 64 4784.5 65 3496.0 66 2493.5 67 1885.0 68 1367.5 69 877.5 70 610.0 71 421.5 72 265.0 73 194.5 74 145.0 75 107.0 76 77.0 77 35.0 78 17.0 79 12.0 80 8.5 81 7.0 82 5.5 83 5.0 84 4.0 85 3.0 86 3.0 87 2.0 88 1.0 89 2.0 90 3.0 91 1.5 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03817805086010521 2 0.028922765803110005 3 0.023369594768912885 4 0.04731764485388797 5 0.09718049309844963 6 0.11291447769534146 7 0.059696588617619055 8 0.08792520804145443 9 0.07288536982383721 10-11 0.0745628902404176 12-13 0.07259614216580612 14-15 0.06652236134715302 16-17 0.05206097844559801 18-19 0.06345654817202336 20-21 0.05934951542798173 22-23 0.03476516449533823 24-25 0.010180813562694723 26-27 0.0027187399854923405 28-29 0.007577764640414822 30-31 0.006015935287046882 32-33 0.007115000387565062 34-35 0.007230691450777501 36-37 0.006478699539896641 38-39 0.004396260402072721 40-41 0.0076934557036272624 42-43 0.0035285774279794208 44-45 0.002371666795855021 46-47 0.0019667480746114806 48-49 2.8922765803110007E-4 50-51 0.0026030489222799006 52-53 0.006073780818653102 54-55 0.012031870574093764 56-57 0.009023902930570323 58-59 0.005263943376166021 60-61 0.0029501221119172206 62-63 0.009255285056995202 64-65 0.0028344310487047808 66-67 0.024179432211399964 68-69 0.04477244146321429 70-71 0.057903377137826235 72-73 0.003123658706735881 74-75 0.013651545459067924 76-77 0.022328375200000927 78-79 0.025278497311918148 80-81 0.07357951620311186 82-83 0.027534473044560727 84-85 0.010874959941969362 86-87 0.03152581472538991 88-89 0.04754902698031285 90-91 0.04303707551502769 92-93 0.042574311262177936 94-95 0.011395569726425343 96-97 0.017295813950259784 98-99 0.03881435170777363 100-101 0.07496780896166114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 864371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.29568287924293 #Duplication Level Percentage of deduplicated Percentage of total 1 78.11668721771883 43.19515563967992 2 12.237811562981376 13.53396294645101 3 3.957813181125034 6.565499476763328 4 1.821779051735466 4.029460668832489 5 0.9274938527703603 2.564320297761854 6 0.6036011552230881 2.002592283885634 7 0.3930090605647082 1.52121930671587 8 0.2821309012121691 1.2480496675090496 9 0.2154419242325691 1.0721707489132224 >10 1.2872612936384025 13.394108510160832 >50 0.10814148223599997 4.07176697680324 >100 0.0456857983437161 4.174416976197373 >500 0.0018861109309855724 0.6767508275363722 >1k 0.0010478394061030959 1.3212864615701567 >5k 2.0956788122061915E-4 0.6292392112196064 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5430 0.6282024732435494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4080 0.4720195379067553 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2586 0.2991770894673699 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2149 0.24862009484353365 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1491 0.1724953752497481 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1096 0.12679740528083427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 904 0.1045847211440458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.1569106321244003E-4 0.0 0.0 0.0 0.0 16-17 1.7353659481866005E-4 0.0 0.0 0.0 0.0 18-19 5.206097844559802E-4 0.0 0.0 0.0 0.0 20-21 8.098374424870802E-4 0.0 0.0 0.0 0.0 22-23 8.098374424870802E-4 0.0 0.0 1.1569106321244003E-4 0.0 24-25 9.255285056995202E-4 0.0 0.0 2.8922765803110007E-4 5.7845531606220015E-5 26-27 9.255285056995202E-4 0.0 0.0 9.833740373057403E-4 1.1569106321244003E-4 28-29 9.833740373057403E-4 0.0 0.0 0.003123658706735881 1.1569106321244003E-4 30-31 0.0011569106321244003 0.0 0.0 0.007404228045596163 1.1569106321244003E-4 32-33 0.0011569106321244003 0.0 0.0 0.010990651005181803 1.1569106321244003E-4 34-35 0.0011569106321244003 0.0 0.0 0.015849675660104284 1.7353659481866005E-4 36-37 0.0012726016953368403 0.0 0.0 0.019956708404145904 2.3138212642488006E-4 38-39 0.0013882927585492804 0.0 0.0 0.023427440300519107 2.3138212642488006E-4 40-41 0.0013882927585492804 0.0 0.0 0.02811292836062293 2.3138212642488006E-4 42-43 0.0013882927585492804 0.0 0.0 0.03470731896373201 2.3138212642488006E-4 44-45 0.0015039838217617203 0.0 0.0 0.039797725745079365 2.3138212642488006E-4 46-47 0.0017932114797928203 0.0 0.0 0.04853240101761859 2.3138212642488006E-4 48-49 0.0018510570113990405 0.0 0.0 0.057498458416582694 2.3138212642488006E-4 50-51 0.0018510570113990405 0.0 0.0 0.06825772729533962 2.3138212642488006E-4 52-53 0.0018510570113990405 0.0 0.0 0.08572707784041805 2.3138212642488006E-4 54-55 0.0018510570113990405 0.0 0.0 0.12182268956269934 2.3138212642488006E-4 56-57 0.0018510570113990405 0.0 0.0 0.1959228155502672 2.3138212642488006E-4 58-59 0.0018510570113990405 0.0 0.0 0.2701964781326537 2.3138212642488006E-4 60-61 0.0018510570113990405 0.0 0.0 0.3320333514197029 2.3138212642488006E-4 62-63 0.0018510570113990405 0.0 0.0 0.4134798599212607 2.3138212642488006E-4 64-65 0.0018510570113990405 0.0 0.0 0.5239648252891409 2.3138212642488006E-4 66-67 0.0018510570113990405 0.0 0.0 0.697790647765832 2.3138212642488006E-4 68-69 0.0019667480746114806 0.0 0.0 0.9141907815047011 2.3138212642488006E-4 70-71 0.0019667480746114806 0.0 0.0 1.091603026940978 2.3138212642488006E-4 72-73 0.0019667480746114806 0.0 0.0 1.2812785250777732 2.3138212642488006E-4 74-75 0.0019667480746114806 0.0 0.0 1.475465974679854 2.3138212642488006E-4 76-77 0.0019667480746114806 0.0 0.0 1.703955824524423 2.3138212642488006E-4 78-79 0.0020824391378239204 0.0 0.0 1.861700589214585 2.3138212642488006E-4 80-81 0.0023138212642488006 0.0 0.0 1.9850272625990462 2.3138212642488006E-4 82-83 0.0024295123274612404 0.0 0.0 2.1576383289120065 2.3138212642488006E-4 84-85 0.0025452033906736803 0.0 0.0 2.4668226953472523 2.3138212642488006E-4 86-87 0.0026608944538861205 0.0 0.0 2.912406825309965 2.3138212642488006E-4 88-89 0.0026608944538861205 0.0 0.0 3.448924130957656 2.3138212642488006E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGCGTA 65 0.005817138 29.257599 5 GGTATCA 5075 0.0 29.133406 1 GTATCAA 7260 0.0 28.616184 1 GTACATG 5240 0.0 28.578945 1 TACATGG 5480 0.0 26.637915 2 ACATGGG 5500 0.0 24.725538 3 CATGGGG 1845 0.0 24.226967 4 ATCAACG 8755 0.0 23.516579 3 GAGTACT 4140 0.0 23.47722 12-13 TATCAAC 8855 0.0 23.465794 2 TCAACGC 8785 0.0 23.3835 4 CAACGCA 8785 0.0 23.325315 5 AACGCAG 8925 0.0 22.95544 6 ATGGGGA 980 0.0 22.316385 5 CCCTATA 215 4.370304E-8 22.115866 2 GTACTTT 4565 0.0 21.601967 14-15 CATGGGA 3580 0.0 21.517883 4 GTATAGG 245 7.4378477E-9 21.344872 1 CGCAGAG 9660 0.0 21.058764 8 ACGCAGA 9695 0.0 21.031767 7 >>END_MODULE