FastQCFastQC Report
Thu 26 May 2016
SRR936932_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936932_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864371
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.8171259794694639No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56060.6485641003689387No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37230.4307178283399142No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27640.31977009871918427No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13870.16046350467565432No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12060.13952342223420267No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9360.10828683516684386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9220.1066671602818697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTACG459.612907E-442.231891
CAGTGCG1004.9478833E-528.503239
GTATCAA91200.027.6104871
GTACATG52050.025.101811
GGTATCA65400.023.53751
TACATGG54650.023.3805472
ACATGGG52750.022.9620293
ATCAACG110300.022.7379323
TCAACGC110800.022.6353244
CATGGGG20050.022.5061784
TATCAAC111250.022.5010682
CATATAG1903.2850585E-722.4999453
AGTACTT59400.022.47308312-13
CAACGCA111600.022.4730635
GAGTACT57500.022.43080112-13
AACGCAG113450.022.109166
TGTAGGA5250.021.7142332
AGAGTAC90800.021.24139210-11
ATGGGGA10600.021.0612685
GTACTTT61600.020.93663214-15
Produced by FastQC (version 0.11.5)