##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936932_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 864371 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27825551759603 33.0 31.0 34.0 31.0 34.0 2 32.39696958829021 34.0 31.0 34.0 31.0 34.0 3 32.51578662403065 34.0 31.0 34.0 31.0 34.0 4 36.171940058146326 37.0 35.0 37.0 35.0 37.0 5 36.03958716801003 37.0 35.0 37.0 35.0 37.0 6 36.14489148756726 37.0 35.0 37.0 35.0 37.0 7 36.10554148623681 37.0 35.0 37.0 35.0 37.0 8 36.05693041529621 37.0 35.0 37.0 35.0 37.0 9 37.7757155203032 39.0 37.0 39.0 35.0 39.0 10-11 37.69657357778084 39.0 37.0 39.0 35.0 39.0 12-13 37.596593360952646 39.0 37.0 39.0 35.0 39.0 14-15 38.788734814101815 40.0 38.0 41.0 35.0 41.0 16-17 38.794926021349625 40.0 38.0 41.0 35.5 41.0 18-19 38.94219322489995 40.0 38.0 41.0 35.0 41.0 20-21 38.96608921400649 40.0 38.0 41.0 35.0 41.0 22-23 38.86959419045757 40.0 38.0 41.0 35.0 41.0 24-25 38.766173899864754 40.0 38.0 41.0 35.0 41.0 26-27 38.56608273530695 40.0 38.0 41.0 34.5 41.0 28-29 38.40376528134331 40.0 38.0 41.0 34.0 41.0 30-31 38.343758640676285 40.0 38.0 41.0 34.0 41.0 32-33 38.16022575954075 40.0 37.0 41.0 34.0 41.0 34-35 38.00275633958104 40.0 37.0 41.0 34.0 41.0 36-37 37.8317956062848 40.0 37.0 41.0 33.0 41.0 38-39 37.64527268962054 40.0 37.0 41.0 33.0 41.0 40-41 37.281133332793445 40.0 36.5 41.0 32.5 41.0 42-43 37.025467073744956 40.0 36.0 41.0 32.0 41.0 44-45 36.696175022068076 39.0 36.0 41.0 31.0 41.0 46-47 36.17205574920954 39.0 35.5 41.0 30.0 41.0 48-49 35.67451071357091 39.0 35.0 40.5 28.5 41.0 50-51 35.3458549627417 39.0 35.0 40.0 27.0 41.0 52-53 35.082362781722196 39.0 35.0 40.0 26.5 41.0 54-55 34.67974284190469 38.0 34.5 40.0 24.5 41.0 56-57 34.47209068791063 38.0 34.0 40.0 25.0 41.0 58-59 34.14011055438001 38.0 34.0 40.0 24.0 41.0 60-61 33.74789181960061 37.0 33.5 40.0 23.0 41.0 62-63 33.37508662368358 37.0 33.0 39.5 22.0 40.5 64-65 33.05144434507868 36.0 33.0 39.0 22.0 40.0 66-67 32.72154086613271 36.0 32.5 39.0 20.5 40.0 68-69 32.266686989730104 35.0 32.0 38.5 18.5 40.0 70-71 31.818071753911227 35.0 31.0 38.0 17.5 40.0 72-73 31.40940059303239 35.0 31.0 37.0 14.0 39.0 74-75 30.92948166932949 34.5 31.0 36.5 9.5 39.0 76-77 29.53248662900537 33.5 29.0 35.0 7.5 37.5 78-79 30.18719508174152 34.0 30.0 36.0 7.0 37.5 80-81 30.039594687929142 34.0 30.0 35.0 4.0 37.0 82-83 29.69680206763068 34.0 30.0 35.0 2.0 37.0 84-85 29.27692507036909 34.0 29.0 35.0 2.0 36.0 86-87 28.925286711377407 34.0 29.0 35.0 2.0 36.0 88-89 28.55559476197142 34.0 29.0 35.0 2.0 35.0 90-91 28.12736776222247 33.5 29.0 35.0 2.0 35.0 92-93 27.673242161062785 33.0 27.0 35.0 2.0 35.0 94-95 27.145125761970263 33.0 26.5 34.5 2.0 35.0 96-97 26.405041353770546 32.5 25.0 34.0 2.0 35.0 98-99 25.59442473197273 32.0 23.5 34.0 2.0 35.0 100-101 22.517475713553555 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.01894567167820327 1101 2 0.029855033151129362 1101 3 0.06682956889922309 1101 4 0.003935467190267161 1101 5 -0.058756443805997094 1101 6 -0.031810587097254484 1101 7 -0.041840231175775955 1101 8 0.008784106834596628 1101 9 0.14153843254432275 1101 10-11 0.018885674182094192 1101 12-13 0.20842247804577596 1101 14-15 0.2609253415847945 1101 16-17 0.31021454106310387 1101 18-19 0.056183246513811014 1101 20-21 -0.0071607082032372205 1101 22-23 -0.05312782080050482 1101 24-25 -0.016230580315912846 1101 26-27 0.029935480142476933 1101 28-29 0.03364993873164934 1101 30-31 0.07454336323253585 1101 32-33 0.1756501001567372 1101 34-35 -0.10902631514122874 1101 36-37 -0.06919780083156013 1101 38-39 0.1339117795575575 1101 40-41 0.2532089490118423 1101 42-43 0.21332817304045193 1101 44-45 0.08509366727741963 1101 46-47 0.07278288674165623 1101 48-49 0.14440045264520052 1101 50-51 0.22943926666029313 1101 52-53 0.17013646330759968 1101 54-55 -0.030938087762599764 1101 56-57 0.2429176256103105 1101 58-59 0.3646596682131076 1101 60-61 0.2650457272635194 1101 62-63 0.16819489945621058 1101 64-65 0.3168206729757159 1101 66-67 0.29630180806091744 1101 68-69 0.0362317167086772 1101 70-71 0.15807580511742358 1101 72-73 0.5594673031572661 1101 74-75 0.2378944158252132 1101 76-77 0.3560776668709984 1101 78-79 0.1855547893290037 1101 80-81 0.3201558627923937 1101 82-83 0.5237491975000772 1101 84-85 0.2393924318699412 1101 86-87 -0.006678368352666553 1101 88-89 0.31785087901797837 1101 90-91 0.3333810799831092 1101 92-93 0.004431989025750482 1101 94-95 -0.18416771408235988 1101 96-97 -0.05517704690013758 1101 98-99 -0.09843211436465893 1101 100-101 0.3333204312547622 1102 1 -0.1384122435670747 1102 2 -0.0902751191640121 1102 3 0.04377044628662219 1102 4 0.10970323548004046 1102 5 -0.09092333137670039 1102 6 0.013530872607674382 1102 7 0.0930705742910547 1102 8 0.11298019719521335 1102 9 0.0922388069947786 1102 10-11 0.10668235555155547 1102 12-13 0.11882778263985472 1102 14-15 0.21151616935931372 1102 16-17 0.13545590588793743 1102 18-19 0.06545125743588898 1102 20-21 0.14005463340131996 1102 22-23 0.08308034323341928 1102 24-25 0.01986369138881372 1102 26-27 0.18007961097560354 1102 28-29 0.0349672148761897 1102 30-31 -0.04619880191677339 1102 32-33 0.059105111781356356 1102 34-35 0.22299245808086 1102 36-37 0.06619917459377689 1102 38-39 0.3164717676794382 1102 40-41 0.29974999008550895 1102 42-43 0.25967637575068636 1102 44-45 0.27877671705687845 1102 46-47 0.11598501756031254 1102 48-49 0.28867433019917854 1102 50-51 0.3191724797638855 1102 52-53 0.3449068679603471 1102 54-55 0.4824383761793456 1102 56-57 0.4694574354155492 1102 58-59 0.3458040597854364 1102 60-61 0.36783400703667724 1102 62-63 0.4497144657600103 1102 64-65 0.5411061751521657 1102 66-67 0.4107301011607447 1102 68-69 0.5126366301939598 1102 70-71 0.39901783410293135 1102 72-73 0.3340039478783865 1102 74-75 0.5817876865811407 1102 76-77 0.5639655707451467 1102 78-79 0.29418435135958276 1102 80-81 0.4140781045700521 1102 82-83 0.6331546397065075 1102 84-85 0.5530707376445925 1102 86-87 0.5772144678379334 1102 88-89 0.6491019201640746 1102 90-91 0.5658454637332646 1102 92-93 0.5996570214370784 1102 94-95 0.4127819994666595 1102 96-97 0.590859536257561 1102 98-99 0.5971195663167386 1102 100-101 0.2513876657534553 1103 1 0.08872853164859151 1103 2 0.19642892145371604 1103 3 0.06099419637476444 1103 4 0.06511893253948386 1103 5 -0.012056067667039372 1103 6 -0.08739642447997653 1103 7 -0.03689205175788146 1103 8 0.10025777517514456 1103 9 0.06444927640220044 1103 10-11 0.06816838145476112 1103 12-13 0.05928802286738488 1103 14-15 0.016939505748382544 1103 16-17 0.010123888869870257 1103 18-19 0.05684525879921409 1103 20-21 0.04691651432180066 1103 22-23 0.10945659784639616 1103 24-25 -0.014507415491287645 1103 26-27 -0.03586660814386988 1103 28-29 0.007650735079280935 1103 30-31 0.04961014432585387 1103 32-33 0.0046131859651268314 1103 34-35 0.06357140173786746 1103 36-37 -0.08998691947574855 1103 38-39 -0.21319527507730385 1103 40-41 -0.1329210721338967 1103 42-43 -0.24387297431864852 1103 44-45 -0.23528258764660137 1103 46-47 -0.435160294241868 1103 48-49 -0.41280417390255764 1103 50-51 -0.324349956359562 1103 52-53 -0.3720177729911569 1103 54-55 -0.3313177059023147 1103 56-57 -0.44897509167468996 1103 58-59 -0.3772202920468217 1103 60-61 -0.3062457475645459 1103 62-63 -0.08059898181068093 1103 64-65 0.009000423324600604 1103 66-67 -0.12123755509292522 1103 68-69 -0.2046141513682258 1103 70-71 -0.10583901661281558 1103 72-73 0.04137648860188747 1103 74-75 -0.09025589285613833 1103 76-77 0.1262936858287098 1103 78-79 0.03121218202502618 1103 80-81 -0.13139353588783464 1103 82-83 0.06059863754398975 1103 84-85 -0.3905803918009134 1103 86-87 -0.30022717943658606 1103 88-89 2.995319921055284E-4 1103 90-91 -0.29454578747140303 1103 92-93 -0.48708168998431134 1103 94-95 -0.0779567824716132 1103 96-97 0.2769859260279084 1103 98-99 0.09178672660162945 1103 100-101 0.6368535473320946 1104 1 0.0918802753553507 1104 2 0.13098924865207806 1104 3 0.08641310046064632 1104 4 0.049230409532214026 1104 5 0.08082700355234351 1104 6 0.069830951966658 1104 7 0.07027406608636255 1104 8 0.06994501471221781 1104 9 0.06939323275145881 1104 10-11 0.10897139291854074 1104 12-13 0.26888239527414015 1104 14-15 0.25732144971838977 1104 16-17 0.23729993561769902 1104 18-19 0.1533935984405943 1104 20-21 0.09448188339918318 1104 22-23 0.17350371914753993 1104 24-25 0.1551174268076707 1104 26-27 0.27079418316241544 1104 28-29 0.17802138378282706 1104 30-31 0.17669327839793425 1104 32-33 0.0876231467505093 1104 34-35 0.2986586895531005 1104 36-37 0.20845975365836011 1104 38-39 0.15569195899431776 1104 40-41 0.6215048049572047 1104 42-43 0.5003154289041447 1104 44-45 0.5799088625654178 1104 46-47 0.41572624753923293 1104 48-49 0.7709124242182455 1104 50-51 0.7486912485903119 1104 52-53 0.6935733383628602 1104 54-55 0.7179009622582413 1104 56-57 0.8444297024372034 1104 58-59 0.6379144541057258 1104 60-61 0.7678896863239828 1104 62-63 0.9558999865853437 1104 64-65 1.0088068552501213 1104 66-67 0.6251358900999762 1104 68-69 0.5828970641937374 1104 70-71 0.5804395119054497 1104 72-73 0.5234031235689365 1104 74-75 0.6278030975838789 1104 76-77 0.7636974141775532 1104 78-79 0.7268753462481818 1104 80-81 0.5994090656670856 1104 82-83 0.6724992027627685 1104 84-85 0.6962667714020263 1104 86-87 0.6907810141861397 1104 88-89 0.6651443814872344 1104 90-91 0.8821283486456686 1104 92-93 0.8542084298457162 1104 94-95 1.0855968541031906 1104 96-97 1.3169953083863426 1104 98-99 0.9144499044884107 1104 100-101 1.502263387877548 1105 1 -0.20157268588447153 1105 2 -0.09973435751636828 1105 3 -0.08296289522767353 1105 4 -0.037359798839098346 1105 5 -0.1749384116183137 1105 6 -0.07626542141613157 1105 7 -0.16638866406130148 1105 8 -0.13594946015879117 1105 9 -0.02994046517222415 1105 10-11 -0.01154619328197981 1105 12-13 6.674084971507455E-4 1105 14-15 -0.11345042504217417 1105 16-17 -0.3574539262659684 1105 18-19 -0.30559461049610803 1105 20-21 -0.02305161003685896 1105 22-23 -0.11004321657623706 1105 24-25 -0.18245492984623723 1105 26-27 -0.24147177228886818 1105 28-29 -0.25552343837369307 1105 30-31 -0.1536625670685936 1105 32-33 -0.16545209360171498 1105 34-35 -0.16686110268324938 1105 36-37 -0.1284924031049144 1105 38-39 -0.17964397931065434 1105 40-41 -0.03084442504382423 1105 42-43 0.09413800021722807 1105 44-45 -0.05440833632390252 1105 46-47 -0.17199339833257454 1105 48-49 -0.3682910779626951 1105 50-51 -0.37024578559928756 1105 52-53 -0.37240166342046166 1105 54-55 -0.14104814568428026 1105 56-57 -0.3199424227810894 1105 58-59 -0.3700318982734885 1105 60-61 -0.6403213903535914 1105 62-63 -0.41493661617233357 1105 64-65 -0.559037332572025 1105 66-67 -0.63950782851024 1105 68-69 -0.36939047606310993 1105 70-71 -0.5696950959089335 1105 72-73 -0.40778441061140924 1105 74-75 -0.6659114176034251 1105 76-77 -0.5195773997624826 1105 78-79 -0.363801972797436 1105 80-81 -0.5527651345458544 1105 82-83 -0.6194769033594447 1105 84-85 -0.8440059316127169 1105 86-87 -0.9375726226026124 1105 88-89 -0.7571272013368606 1105 90-91 -0.6789511029893056 1105 92-93 -0.5138521344239031 1105 94-95 -0.171556910556383 1105 96-97 -0.2957403873867861 1105 98-99 -0.2873805768501114 1105 100-101 -0.1486426719839642 1106 1 -0.09358856696999851 1106 2 -0.06671483298057268 1106 3 -0.05928198158107989 1106 4 0.013365562479805249 1106 5 -0.005688950267938253 1106 6 0.05002219327387536 1106 7 0.11833154074660257 1106 8 0.13924300116737953 1106 9 0.03471867250419791 1106 10-11 0.19880253695745154 1106 12-13 0.2206218048464308 1106 14-15 0.22987667448090576 1106 16-17 0.05969011106427047 1106 18-19 0.1775544320390594 1106 20-21 0.1251125933669215 1106 22-23 0.18568072246556966 1106 24-25 0.1601527092948274 1106 26-27 0.25630478495325093 1106 28-29 0.15289326460642627 1106 30-31 0.11417339293818429 1106 32-33 -0.02564301160592919 1106 34-35 0.07956353478740397 1106 36-37 0.05408182765499703 1106 38-39 0.002295522468706679 1106 40-41 0.07785764119192606 1106 42-43 -0.010780657637383229 1106 44-45 -0.14575559586829456 1106 46-47 -0.3873821338979795 1106 48-49 -0.5996403347472565 1106 50-51 -0.43212013498315827 1106 52-53 -0.5680994265114663 1106 54-55 -0.19425643967802841 1106 56-57 -0.3449949259887788 1106 58-59 -0.5564190420989945 1106 60-61 -0.7794874215347534 1106 62-63 -0.6465499020560799 1106 64-65 -0.5621686117741049 1106 66-67 -0.6685396814649494 1106 68-69 -0.655779586867574 1106 70-71 -0.649876723720201 1106 72-73 -0.6471786113522029 1106 74-75 -0.8620752034024406 1106 76-77 -0.6374917363156065 1106 78-79 -0.627479285359474 1106 80-81 -0.40824967711733606 1106 82-83 -0.5882147988735191 1106 84-85 -0.42691939059060857 1106 86-87 -0.3283757855047824 1106 88-89 -0.376997168896839 1106 90-91 -0.473121128650817 1106 92-93 -0.2701041014028611 1106 94-95 -0.3615504346564151 1106 96-97 -0.31012959502713855 1106 98-99 -0.0496227512999603 1106 100-101 0.29456686271959853 1107 1 0.2479154259315095 1107 2 0.3144131616955548 1107 3 0.30501482409771086 1107 4 0.11357629894830268 1107 5 -0.03754897688727965 1107 6 0.11457410099347953 1107 7 0.20745088413612933 1107 8 0.11606198963232828 1107 9 0.23422510551218778 1107 10-11 0.23583392559986294 1107 12-13 0.300341192601536 1107 14-15 0.44446740207629176 1107 16-17 0.42121406847331855 1107 18-19 0.24374232023782127 1107 20-21 0.31476155077596957 1107 22-23 0.14270157428455832 1107 24-25 0.20074720796385748 1107 26-27 0.19265405292130566 1107 28-29 0.299452159903673 1107 30-31 0.2100584861058863 1107 32-33 0.2341035500622226 1107 34-35 0.2047437699249386 1107 36-37 0.2047861311155188 1107 38-39 0.044415087686097365 1107 40-41 0.31101258795323616 1107 42-43 0.22136219950547797 1107 44-45 0.27832381850615207 1107 46-47 0.12355675252615583 1107 48-49 0.2670965774089069 1107 50-51 0.2194853441650153 1107 52-53 0.1009611324947457 1107 54-55 0.2743549179084823 1107 56-57 0.254023485723728 1107 58-59 0.12177805195602076 1107 60-61 0.07367639834102135 1107 62-63 0.27328705446566204 1107 64-65 0.30736887713450756 1107 66-67 0.018655305225379948 1107 68-69 -0.08070471996429518 1107 70-71 -0.15102745575214627 1107 72-73 -0.05578553238148132 1107 74-75 0.047086287280787786 1107 76-77 -0.26647077358268945 1107 78-79 -0.1012481407277086 1107 80-81 -0.06943534703747645 1107 82-83 0.20927022774582227 1107 84-85 0.27002215776786187 1107 86-87 0.24730910801784134 1107 88-89 0.38170957467015043 1107 90-91 0.09144464331014035 1107 92-93 0.2531323670975816 1107 94-95 0.37759386436754383 1107 96-97 0.34377734818493266 1107 98-99 0.5871479896050964 1107 100-101 0.6901552611224382 1108 1 0.015665479476162147 1108 2 0.023846391509223963 1108 3 -0.00795369164441695 1108 4 0.0551055343306146 1108 5 -0.0023902169815173124 1108 6 -0.012741705389039737 1108 7 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-0.5726642884825424 2316 78-79 -0.9196012184023523 2316 80-81 -0.818430828229463 2316 82-83 -0.5356537463626303 2316 84-85 -0.5805381133606176 2316 86-87 -0.5763306697056514 2316 88-89 -1.2277524429165574 2316 90-91 -1.1263786957225896 2316 92-93 -0.9748048581100406 2316 94-95 -1.057104779990052 2316 96-97 -0.6652373684735942 2316 98-99 -0.9626214901213324 2316 100-101 -1.7138185420040877 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 21.0 6 51.0 7 96.0 8 215.0 9 460.0 10 791.0 11 1430.0 12 2363.0 13 3444.0 14 4361.0 15 6144.0 16 9001.0 17 13210.0 18 11493.0 19 6031.0 20 4629.0 21 4651.0 22 4706.0 23 4971.0 24 5710.0 25 6763.0 26 7833.0 27 9909.0 28 12337.0 29 15355.0 30 19305.0 31 24938.0 32 32274.0 33 43015.0 34 60493.0 35 89403.0 36 138128.0 37 197035.0 38 118211.0 39 5586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.07337636339652 19.797454761733945 15.324889319858089 20.804279555011444 2 15.682504202633456 21.71219857023691 39.58203899164564 23.023258235483997 3 19.234493601690943 26.665926242148895 30.86251357926996 23.237066576890193 4 12.899206474997428 17.830538044427684 37.72199668892177 31.548258791653122 5 13.095450201655357 38.32446728509185 33.67806410858615 14.90201840466665 6 30.41737864875152 37.349124392188074 17.416711111316786 14.816785847743619 7 27.142111760139475 34.47474400400755 21.20425420161991 17.178890034233067 8 26.746501213020796 33.58800792715165 19.956129948829844 19.70936091099771 9 27.51969642629892 16.37921260571283 20.828348970926804 35.27274199706144 10-11 24.73330317652952 27.323336854198022 26.96590931440319 20.977450654869266 12-13 26.52529591883369 25.0722695084285 27.84954752374611 20.552887048991696 14-15 23.48970110737609 25.444891220531723 27.539378288996062 23.526029383096127 16-17 21.260546686550104 28.923807022678922 27.82057704388509 21.995069246885887 18-19 21.080400687441468 26.64923189233246 31.709249974981617 20.561117445244452 20-21 22.339675991110177 25.360816512583312 31.846200479199037 20.453307017107473 22-23 22.029149992422113 25.63009265847329 31.66573533852332 20.675022010581277 24-25 21.882127682958927 25.671488111248976 31.88932948080533 20.557054724986767 26-27 21.576916380186507 25.90237635365853 31.94845266005723 20.572254606097733 28-29 21.18118261718637 26.158674920838386 32.01894788233293 20.641194579642306 30-31 21.325565064075494 25.867769742390713 31.783863641885258 21.02280155164854 32-33 21.477979890532865 26.077230904856606 31.583559799113576 20.86122940549696 34-35 21.83819216516982 25.821030552852886 31.629589609091468 20.711187672885835 36-37 21.830653502368232 25.702371008309054 31.43662695318375 21.03034853613896 38-39 21.63054941533036 26.046999189509556 31.53056578712159 20.79188560803849 40-41 21.6834149343798 25.998012420635657 31.34781713344316 20.97075551154138 42-43 21.46427864886721 26.391445340021818 30.69237630600749 21.45189970510348 44-45 21.87380331225813 26.233709139078975 30.506094069056477 21.386393479606415 46-47 22.32019986822765 26.70275848465269 29.815894707066825 21.161146940052834 48-49 22.3243647537309 27.05625314174089 29.22986060938059 21.38952149514762 50-51 21.98550708708879 27.31631213386653 29.17972797009557 21.518452808949114 52-53 22.433018864104522 27.591594537733705 28.511632507738327 21.46375409042345 54-55 21.952494935623708 27.326576203967974 28.66309721172969 22.057831648678636 56-57 22.286424632129638 27.61159322371169 28.429359288357837 21.672622855800835 58-59 22.687911426819532 26.997003597996226 28.494279070305538 21.8208059048787 60-61 22.27464437169875 27.17173183795231 29.201868917759636 21.3517548725893 62-63 22.095154797624396 27.188167573974354 29.362061754768355 21.35461587363289 64-65 22.066534007427375 27.44091072110323 28.883869176394366 21.608686095075026 66-67 22.09924904930869 27.42514498982497 28.591137370411545 21.88446859045479 68-69 22.06495941265927 27.31074232635195 28.787076837999447 21.83722142298933 70-71 22.17504581382004 27.417488847342796 28.675575680728578 21.73188965810858 72-73 22.158097014234677 27.264833959609795 28.669617570294132 21.90745145586139 74-75 22.01991103089623 28.056852298561342 28.13355778074727 21.789678889795162 76-77 22.127143395106614 27.649196727560692 28.314015806879112 21.909644070453584 78-79 22.137876619537767 27.66799287802519 28.073202702092974 22.120927800344067 80-81 22.11019231158584 27.904240457452257 28.014033626327222 21.971533604634683 82-83 22.126217866065538 27.613101094958704 28.19890313239519 22.061777906580566 84-85 21.95267415439152 27.881246905181712 27.85249691096642 22.313582029460356 86-87 22.052875627047737 27.77260379838217 27.887890343002052 22.286630231568036 88-89 22.01379886422864 27.65250972844802 27.448193519612797 22.885497887710542 90-91 22.00334467313658 27.431988657497257 27.796536047790298 22.76813062157586 92-93 22.1207675870662 27.562412436326532 27.77939102538146 22.537428951225806 94-95 21.931439162119045 27.362209051437404 27.703844761103742 23.002507025339813 96-97 21.909649593691576 27.510157710604744 27.815698869000236 22.76449382670344 98-99 21.748687616596047 27.998843077991005 27.243430852217678 23.009038453195274 100-101 21.28832517495867 28.285977738107498 27.186650731708443 23.23904635522539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 513.0 1 378.0 2 287.5 3 334.0 4 311.0 5 307.5 6 469.0 7 971.0 8 1632.0 9 1973.0 10 1903.0 11 1762.0 12 1806.0 13 1946.0 14 2085.0 15 2189.5 16 2275.5 17 2376.0 18 2465.5 19 2591.0 20 2733.5 21 2934.0 22 3197.0 23 3572.5 24 4086.0 25 4672.0 26 5393.5 27 6385.0 28 7518.0 29 8625.0 30 9896.5 31 11537.0 32 13422.5 33 15322.5 34 17311.0 35 19027.5 36 21524.0 37 23935.0 38 25174.0 39 25580.0 40 25286.5 41 25938.0 42 26576.0 43 27432.5 44 29727.0 45 30801.0 46 30134.5 47 31482.5 48 35980.5 49 35900.5 50 31650.0 51 29254.0 52 27839.5 53 27399.0 54 26536.5 55 24488.0 56 22226.5 57 20027.0 58 18780.5 59 17421.5 60 14659.5 61 11533.0 62 8853.5 63 6718.5 64 4881.5 65 3598.0 66 2602.5 67 1999.0 68 1476.0 69 922.5 70 642.0 71 431.5 72 283.5 73 218.5 74 164.5 75 127.0 76 97.0 77 48.5 78 23.0 79 13.5 80 8.5 81 6.5 82 5.5 83 4.0 84 2.0 85 1.0 86 0.5 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019551789682902363 2 0.003702114022798081 3 2.3138212642488006E-4 4 0.0 5 0.0028922765803110007 6 0.0 7 2.3138212642488006E-4 8 0.0 9 1.1569106321244003E-4 10-11 0.0 12-13 0.008271911019689462 14-15 0.0035285774279794208 16-17 0.0 18-19 7.519919108808602E-4 20-21 0.0013882927585492802 22-23 0.0016196748849741603 24-25 4.049187212435401E-4 26-27 5.7845531606220015E-5 28-29 0.0 30-31 0.0 32-33 2.3138212642488006E-4 34-35 0.0 36-37 0.0015039838217617205 38-39 0.009197439525388982 40-41 3.4707318963732005E-4 42-43 0.0 44-45 6.941463792746401E-4 46-47 1.7353659481866003E-4 48-49 1.7353659481866003E-4 50-51 0.0020245936062177005 52-53 5.206097844559801E-4 54-55 0.0 56-57 4.627642528497601E-4 58-59 1.1569106321244003E-4 60-61 0.002371666795855021 62-63 5.7845531606220015E-5 64-65 1.1569106321244003E-4 66-67 0.0 68-69 5.7845531606220015E-5 70-71 3.4707318963732005E-4 72-73 3.4707318963732005E-4 74-75 0.003007967643523441 76-77 5.7845531606220015E-5 78-79 4.627642528497601E-4 80-81 0.0021402846694301403 82-83 5.7845531606220015E-5 84-85 0.002660894453886121 86-87 3.4707318963732005E-4 88-89 0.0028344310487047808 90-91 0.0013304472269430605 92-93 0.0 94-95 0.0 96-97 3.4707318963732005E-4 98-99 9.833740373057403E-4 100-101 0.0027765855170985604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 864371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.2922541497144 #Duplication Level Percentage of deduplicated Percentage of total 1 76.43867971010707 39.97150866269537 2 12.877847355820684 13.468233336636057 3 4.354556908132122 6.831287896483183 4 1.9815330761401988 4.144753248943546 5 1.096048571916144 2.865742524153526 6 0.6636151445094182 2.0821159076571565 7 0.44894883042123845 1.6433582438423102 8 0.32523572513144416 1.3605847357712106 9 0.2511296544039318 1.1818922141358312 >10 1.3829575952212494 13.557754861131757 >50 0.12074869763828687 4.29761579950825 >100 0.05445813786148347 5.005484466069167 >500 0.0029014581609305844 1.051329137158273 >1k 8.927563572094106E-4 1.0597323007767672 >5k 4.463781786047053E-4 1.4786066650375398 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.8171259794694639 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5606 0.6485641003689387 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.4307178283399142 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2764 0.31977009871918427 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1387 0.16046350467565432 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1206 0.13952342223420267 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 936 0.10828683516684386 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 922 0.1066671602818697 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.1569106321244003E-4 0.0 0.0 1.1569106321244003E-4 0.0 16-17 1.1569106321244003E-4 0.0 0.0 1.1569106321244003E-4 0.0 18-19 1.1569106321244003E-4 0.0 0.0 1.1569106321244003E-4 1.1569106321244003E-4 20-21 1.1569106321244003E-4 0.0 0.0 1.1569106321244003E-4 1.1569106321244003E-4 22-23 1.1569106321244003E-4 0.0 0.0 2.3138212642488006E-4 1.1569106321244003E-4 24-25 1.1569106321244003E-4 0.0 0.0 5.206097844559801E-4 1.1569106321244003E-4 26-27 1.1569106321244003E-4 0.0 0.0 0.0012147561637306202 1.1569106321244003E-4 28-29 1.1569106321244003E-4 0.0 0.0 0.003470731896373201 1.1569106321244003E-4 30-31 1.1569106321244003E-4 0.0 0.0 0.008040528893264582 1.1569106321244003E-4 32-33 1.1569106321244003E-4 0.0 0.0 0.011800488447668882 1.1569106321244003E-4 34-35 2.3138212642488006E-4 0.0 0.0 0.016543822039378923 1.1569106321244003E-4 36-37 3.470731896373201E-4 0.0 0.0 0.020766545846632983 1.1569106321244003E-4 38-39 5.784553160622001E-4 0.0 0.0 0.024237277743006186 1.1569106321244003E-4 40-41 5.784553160622001E-4 0.0 0.0 0.02880707473989757 1.1569106321244003E-4 42-43 6.941463792746402E-4 0.0 0.0 0.03563284746943153 1.1569106321244003E-4 44-45 9.255285056995202E-4 0.0 0.0 0.040376181061141575 1.1569106321244003E-4 46-47 0.0011569106321244003 0.0 0.0 0.04887947420725591 1.7353659481866005E-4 48-49 0.0012726016953368403 0.0 0.0 0.057729840543007574 3.470731896373201E-4 50-51 0.0012726016953368403 0.0 0.0 0.06831557282694584 3.470731896373201E-4 52-53 0.0013304472269430605 0.0 0.0 0.08566923230881185 3.470731896373201E-4 54-55 0.0015039838217617203 0.0 0.0 0.12130207977824338 3.470731896373201E-4 56-57 0.0016196748849741603 0.0 0.0 0.19574927895544852 3.470731896373201E-4 58-59 0.0016196748849741603 0.0 0.0 0.271064161106747 3.470731896373201E-4 60-61 0.0016196748849741603 0.0 0.0 0.3332481075834335 3.470731896373201E-4 62-63 0.0016196748849741603 0.0 0.0 0.4149838437430224 3.470731896373201E-4 64-65 0.0016196748849741603 0.0 0.0 0.5256423457057213 3.470731896373201E-4 66-67 0.0016196748849741603 0.0 0.0 0.7003936966881119 3.470731896373201E-4 68-69 0.0016196748849741603 0.0 0.0 0.9174301312746495 3.470731896373201E-4 70-71 0.0017932114797928203 0.0 0.0 1.095594368621807 3.470731896373201E-4 72-73 0.0019089025430052606 0.0 0.0 1.2870052327067891 4.0491872124354014E-4 74-75 0.0019667480746114806 0.0 0.0 1.4820025197513567 4.627642528497601E-4 76-77 0.0019667480746114806 0.0 0.0 1.7130954185182057 4.627642528497601E-4 78-79 0.0019667480746114806 0.0 0.0 1.871129410866399 4.627642528497601E-4 80-81 0.0023138212642488006 0.0 0.0 1.9946296208456784 4.627642528497601E-4 82-83 0.0023138212642488006 0.0 0.0 2.168166215664338 5.784553160622001E-4 84-85 0.0023716667958550205 0.0 0.0 2.4792016391109835 5.784553160622001E-4 86-87 0.0024295123274612404 0.0 0.0 2.9250171512001213 5.784553160622001E-4 88-89 0.0024295123274612404 0.0 0.0 3.4624599853535116 5.784553160622001E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTACG 45 9.612907E-4 42.23189 1 CAGTGCG 100 4.9478833E-5 28.50323 9 GTATCAA 9120 0.0 27.610487 1 GTACATG 5205 0.0 25.10181 1 GGTATCA 6540 0.0 23.5375 1 TACATGG 5465 0.0 23.380547 2 ACATGGG 5275 0.0 22.962029 3 ATCAACG 11030 0.0 22.737932 3 TCAACGC 11080 0.0 22.635324 4 CATGGGG 2005 0.0 22.506178 4 TATCAAC 11125 0.0 22.501068 2 CATATAG 190 3.2850585E-7 22.499945 3 AGTACTT 5940 0.0 22.473083 12-13 CAACGCA 11160 0.0 22.473063 5 GAGTACT 5750 0.0 22.430801 12-13 AACGCAG 11345 0.0 22.10916 6 TGTAGGA 525 0.0 21.714233 2 AGAGTAC 9080 0.0 21.241392 10-11 ATGGGGA 1060 0.0 21.061268 5 GTACTTT 6160 0.0 20.936632 14-15 >>END_MODULE