##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936931_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 107851 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18536684870794 33.0 31.0 34.0 31.0 34.0 2 32.190874447153945 33.0 31.0 34.0 30.0 34.0 3 32.334507793159084 33.0 31.0 34.0 31.0 34.0 4 36.09064357307767 37.0 35.0 37.0 35.0 37.0 5 36.060898832648746 37.0 35.0 37.0 35.0 37.0 6 36.15217290521182 37.0 35.0 37.0 35.0 37.0 7 36.09158932230577 37.0 35.0 37.0 35.0 37.0 8 36.105766288676044 37.0 35.0 37.0 35.0 37.0 9 37.77521766140323 39.0 37.0 39.0 35.0 39.0 10-11 37.70320627532429 39.0 37.0 39.0 35.0 39.0 12-13 37.61424557954956 39.0 37.0 39.0 35.0 39.0 14-15 38.69534821188492 40.0 38.0 41.0 34.5 41.0 16-17 38.658978590833655 40.0 38.0 41.0 34.5 41.0 18-19 38.769682246803455 40.0 38.0 41.0 35.0 41.0 20-21 38.854660596563775 40.0 38.0 41.0 35.0 41.0 22-23 38.76259839964395 40.0 38.0 41.0 35.0 41.0 24-25 38.59673994677843 40.0 38.0 41.0 35.0 41.0 26-27 38.36648709794068 40.0 38.0 41.0 34.0 41.0 28-29 38.145353311513105 40.0 37.0 41.0 34.0 41.0 30-31 37.86056225718816 40.0 37.0 41.0 34.0 41.0 32-33 37.62597472438827 40.0 35.5 41.0 33.0 41.0 34-35 37.42126637676053 40.0 35.0 41.0 33.0 41.0 36-37 37.15150068149577 39.5 35.0 41.0 33.0 41.0 38-39 36.90492438642201 39.0 35.0 41.0 33.0 41.0 40-41 36.60702265162122 39.0 35.0 41.0 32.0 41.0 42-43 36.21303001362992 39.0 35.0 41.0 31.0 41.0 44-45 35.51766789366812 39.0 35.0 41.0 29.0 41.0 46-47 34.93714476453626 39.0 35.0 40.5 25.0 41.0 48-49 34.29164310020306 38.0 35.0 40.0 21.0 41.0 50-51 33.89032554171959 38.0 34.0 40.0 16.0 41.0 52-53 33.60272505586411 38.0 34.0 40.0 9.0 41.0 54-55 33.308527505540056 38.0 34.0 40.0 4.0 41.0 56-57 32.941405272088346 37.5 33.0 40.0 2.0 41.0 58-59 32.570847743646326 37.0 33.0 40.0 2.0 41.0 60-61 32.14740243484066 36.5 32.5 40.0 2.0 41.0 62-63 31.73642803497418 36.0 32.0 39.0 2.0 41.0 64-65 31.28904228982578 35.5 31.0 39.0 2.0 40.5 66-67 30.864632687689497 35.0 31.0 39.0 2.0 40.0 68-69 30.322815736525392 35.0 30.5 38.0 2.0 40.0 70-71 29.769686882829088 35.0 29.5 37.5 2.0 40.0 72-73 29.41665816728635 35.0 29.0 37.0 2.0 39.0 74-75 28.96954131162437 34.5 29.0 36.5 2.0 39.0 76-77 27.615645659289203 32.5 26.5 35.0 2.0 37.5 78-79 28.21965489425226 34.0 28.5 35.5 2.0 38.0 80-81 27.965099999072798 34.0 28.0 35.0 2.0 37.0 82-83 27.606874298801124 34.0 27.0 35.0 2.0 37.0 84-85 27.188630610750018 34.0 26.0 35.0 2.0 36.0 86-87 26.834883311234943 34.0 25.5 35.0 2.0 36.0 88-89 26.45848439050171 33.5 25.0 35.0 2.0 35.5 90-91 26.002572994223513 33.0 23.5 35.0 2.0 35.0 92-93 25.55806158496444 33.0 20.0 35.0 2.0 35.0 94-95 25.05946630072971 33.0 16.5 35.0 2.0 35.0 96-97 24.39790080759566 32.0 3.5 34.5 2.0 35.0 98-99 23.811953528479105 32.0 2.0 34.0 2.0 35.0 100-101 21.33801726455944 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5152465463550513 1101 2 0.3739245703826555 1101 3 0.39222789372956157 1101 4 0.2849233883322455 1101 5 0.18850253607369893 1101 6 0.2536347898801097 1101 7 0.373827638462771 1101 8 0.31733017048983214 1101 9 0.36820552130087236 1101 10-11 0.40000185590078274 1101 12-13 0.4132915082054254 1101 14-15 0.6206357051002414 1101 16-17 0.6328081502332452 1101 18-19 0.5641356144029857 1101 20-21 0.44479303633089273 1101 22-23 0.40943136225772037 1101 24-25 0.5330804695615683 1101 26-27 0.5236315271886625 1101 28-29 0.39287405025184086 1101 30-31 0.4413792270581496 1101 32-33 0.7079223234137899 1101 34-35 0.5560534298525823 1101 36-37 1.0224039555720665 1101 38-39 1.0682300653233199 1101 40-41 1.0332622452069131 1101 42-43 1.0727780354463476 1101 44-45 1.2446211111252623 1101 46-47 1.2943961374980404 1101 48-49 0.9426188878952075 1101 50-51 1.2953761081526523 1101 52-53 1.388253162146249 1101 54-55 1.9615577561911195 1101 56-57 1.9463148894623856 1101 58-59 1.9437993769180117 1101 60-61 2.2351595894916123 1101 62-63 2.5120433896211267 1101 64-65 2.320890893933637 1101 66-67 2.452712332715663 1101 68-69 2.61965816922271 1101 70-71 2.5810259407736424 1101 72-73 2.5145733163668105 1101 74-75 2.3556032368139626 1101 76-77 2.218455950161559 1101 78-79 2.600386648526815 1101 80-81 2.8958873490335435 1101 82-83 3.0610096581088264 1101 84-85 3.040676040255871 1101 86-87 2.808060758754742 1101 88-89 2.7627156112861897 1101 90-91 2.777330635669859 1101 92-93 2.639576882556984 1101 94-95 2.8493262880831765 1101 96-97 3.1044306155709798 1101 98-99 3.1876928005440384 1101 100-101 2.4658448392707655 1102 1 0.29978556648558197 1102 2 0.15701913001917944 1102 3 0.20305609485098586 1102 4 0.19443450411231566 1102 5 0.10155914579360825 1102 6 0.06937415668114966 1102 7 0.3158785097350787 1102 8 0.3348212739263374 1102 9 0.45384889713636767 1102 10-11 0.3527483005582468 1102 12-13 0.12724237028571395 1102 14-15 0.36017978084249336 1102 16-17 0.47239424664442 1102 18-19 0.19633001252255866 1102 20-21 0.3415797683046833 1102 22-23 0.4317482768900973 1102 24-25 0.22340783683534227 1102 26-27 0.12652964019800095 1102 28-29 0.3774314544250146 1102 30-31 0.4868403382422102 1102 32-33 0.8123830800484626 1102 34-35 0.8024718244004063 1102 36-37 0.6787536725891314 1102 38-39 0.8138934009765393 1102 40-41 1.2171223689052582 1102 42-43 1.5475590683669225 1102 44-45 1.7754537769427614 1102 46-47 1.6775392835329939 1102 48-49 1.9523230361231185 1102 50-51 2.1130523651615647 1102 52-53 2.2008147158604814 1102 54-55 2.6814440377558597 1102 56-57 2.5742087347475433 1102 58-59 2.9467342580126434 1102 60-61 2.8477815479174104 1102 62-63 3.240136833083639 1102 64-65 3.1946450467575573 1102 66-67 2.8893136484878568 1102 68-69 2.9071911620135396 1102 70-71 3.081932011447158 1102 72-73 3.422259159340822 1102 74-75 2.8965536918190153 1102 76-77 2.582945858635288 1102 78-79 2.971777138724182 1102 80-81 2.9530485902190478 1102 82-83 2.630507905059048 1102 84-85 3.3656336913004807 1102 86-87 3.524401546379657 1102 88-89 3.718653000489507 1102 90-91 3.4694767100201815 1102 92-93 3.335531080027863 1102 94-95 2.921588707160563 1102 96-97 3.399796523677029 1102 98-99 3.351396134621485 1102 100-101 2.810854228263974 1103 1 0.10751295615276746 1103 2 -0.2853177972675809 1103 3 -0.02857849129864576 1103 4 -0.0565419441885453 1103 5 -0.04073334432496978 1103 6 0.12227777480216417 1103 7 0.16928263069772953 1103 8 0.3656700238386321 1103 9 0.1793675256650289 1103 10-11 0.0982168788027451 1103 12-13 0.31332885904778607 1103 14-15 0.24290073881429208 1103 16-17 0.38956570394964984 1103 18-19 0.3110246824011753 1103 20-21 0.502126579353174 1103 22-23 0.45618871939074523 1103 24-25 0.3876382552463582 1103 26-27 0.31115438000669116 1103 28-29 0.6458670322251407 1103 30-31 0.9628559614150234 1103 32-33 1.071404994588768 1103 34-35 0.5757019387676436 1103 36-37 0.6520212077295753 1103 38-39 0.961249389469657 1103 40-41 1.2480295887756867 1103 42-43 1.3202863410941887 1103 44-45 1.501732514927184 1103 46-47 1.53668515438455 1103 48-49 1.705942326531705 1103 50-51 1.9819042490636178 1103 52-53 2.4745827538425686 1103 54-55 2.545793734881478 1103 56-57 2.4839311193863125 1103 58-59 2.372302001306142 1103 60-61 2.2883059065019715 1103 62-63 2.705510923875785 1103 64-65 2.7834024432072653 1103 66-67 3.1532470395115553 1103 68-69 3.4135994694251934 1103 70-71 3.05664960154936 1103 72-73 3.119735595947695 1103 74-75 3.187652225392469 1103 76-77 2.78557934554971 1103 78-79 2.8904454269576867 1103 80-81 3.1563603286478426 1103 82-83 3.3922428256568438 1103 84-85 3.000523884719101 1103 86-87 2.7859844450862994 1103 88-89 3.173771460624689 1103 90-91 3.924139104481906 1103 92-93 3.509163467707072 1103 94-95 3.2045131405866663 1103 96-97 2.543784642581535 1103 98-99 2.3978797513392145 1103 100-101 2.592562486560862 1104 1 0.43893135105137304 1104 2 0.14462418009784983 1104 3 0.269175285917413 1104 4 0.12413249712816565 1104 5 0.16315015082066253 1104 6 0.25073184500380563 1104 7 0.16652511935171788 1104 8 0.4411568969356594 1104 9 0.21412365825536028 1104 10-11 0.3344436841109584 1104 12-13 0.3080436289679298 1104 14-15 0.5356278026391905 1104 16-17 0.48803183826343144 1104 18-19 0.4436150196218378 1104 20-21 0.18541491163328772 1104 22-23 0.26965456646376396 1104 24-25 0.5125018158828851 1104 26-27 0.4205931115514687 1104 28-29 0.3820363893803105 1104 30-31 0.14714963637337064 1104 32-33 0.2287267616939559 1104 34-35 0.06800388626002984 1104 36-37 0.5817046684063101 1104 38-39 0.7758067514504603 1104 40-41 0.3822284742815114 1104 42-43 0.44164556439515934 1104 44-45 1.1638224787348506 1104 46-47 1.6916802712915455 1104 48-49 1.076138224733576 1104 50-51 1.2131905132102219 1104 52-53 0.0643167688939883 1104 54-55 -0.5107352477117288 1104 56-57 -0.6479985640743635 1104 58-59 -0.8244004747092539 1104 60-61 -0.9325822419440577 1104 62-63 -0.7181117566550341 1104 64-65 -1.5335102930482662 1104 66-67 -1.1750978791993134 1104 68-69 -1.6544765919294377 1104 70-71 -1.7896478649870886 1104 72-73 -2.185342820781205 1104 74-75 -2.249499495179716 1104 76-77 -1.992555887402549 1104 78-79 -2.8362162608690475 1104 80-81 -2.264016531236109 1104 82-83 -2.5630912353530952 1104 84-85 -2.5120285367205497 1104 86-87 -2.4501561879923486 1104 88-89 -2.2158993614583835 1104 90-91 -1.5846504629334994 1104 92-93 -1.788188172437696 1104 94-95 -2.1697041283281386 1104 96-97 -1.8215281626117772 1104 98-99 -2.0959732962853046 1104 100-101 -1.6785927957968063 1105 1 -0.31183963695547945 1105 2 -0.3666265337799359 1105 3 -0.4592084947793289 1105 4 -0.23886007854287783 1105 5 -0.48779216785437995 1105 6 -0.3673149853788331 1105 7 -0.1258792210252082 1105 8 -0.024862520540068545 1105 9 0.021205325873864922 1105 10-11 -0.07703547065774785 1105 12-13 -0.5386725332251032 1105 14-15 -0.3355886451049557 1105 16-17 -0.6981474878359322 1105 18-19 -0.1711194178424691 1105 20-21 -0.22589291246723064 1105 22-23 -0.0945944341073428 1105 24-25 0.0470535006344619 1105 26-27 -0.20990374738627793 1105 28-29 -0.17278942444036716 1105 30-31 0.08558481627057546 1105 32-33 -0.5399768362500765 1105 34-35 -0.5345660737628108 1105 36-37 -0.1628413053229849 1105 38-39 0.08220309639621348 1105 40-41 -0.1482112744334998 1105 42-43 -0.020890129494048892 1105 44-45 -0.10710841789565251 1105 46-47 0.005672275860355569 1105 48-49 0.16671503798314546 1105 50-51 0.07978103291324601 1105 52-53 0.7247393845421826 1105 54-55 1.0185051863259602 1105 56-57 0.19169304072289606 1105 58-59 0.6095801078150167 1105 60-61 0.934807820877186 1105 62-63 0.9965655705865402 1105 64-65 1.5240568114634385 1105 66-67 0.968248779852658 1105 68-69 1.5738500785445773 1105 70-71 1.2225737226771045 1105 72-73 2.119283107259914 1105 74-75 2.089392566499324 1105 76-77 1.8240060771891144 1105 78-79 1.271893390758592 1105 80-81 2.0438646795006115 1105 82-83 1.571289118730288 1105 84-85 0.596138225129824 1105 86-87 0.7586958965307815 1105 88-89 0.7637122890270547 1105 90-91 0.8186048197621041 1105 92-93 0.5671516333875459 1105 94-95 0.3348646894902494 1105 96-97 0.33118457296789217 1105 98-99 1.3538839281578738 1105 100-101 1.7781176553739506 1106 1 0.41757212775040387 1106 2 0.6671969956318264 1106 3 0.589320916985379 1106 4 0.4993752155747657 1106 5 0.3342666556750302 1106 6 0.3415085440329335 1106 7 0.19176783779832363 1106 8 0.12513747945993003 1106 9 0.29179356116798516 1106 10-11 0.20531747051872884 1106 12-13 0.17309217265725607 1106 14-15 0.9872054725421009 1106 16-17 0.8783231003993635 1106 18-19 0.3465276409810585 1106 20-21 0.28881296988571137 1106 22-23 0.9980526247161876 1106 24-25 0.913229971222691 1106 26-27 0.5687727232019526 1106 28-29 0.9992693990890444 1106 30-31 1.3892612868588117 1106 32-33 1.361493751985222 1106 34-35 0.8426398085901283 1106 36-37 1.42539398879466 1106 38-39 1.5983795669844483 1106 40-41 1.849582843213561 1106 42-43 2.307786341094186 1106 44-45 1.6862739350455271 1106 46-47 1.4394958052721236 1106 48-49 2.11230327327727 1106 50-51 2.0084575035014893 1106 52-53 1.4887099727774782 1106 54-55 1.9442404804436038 1106 56-57 2.153457746605241 1106 58-59 2.4345801078150124 1106 60-61 1.9561313502889526 1106 62-63 2.5098008647041894 1106 64-65 2.594645046757563 1106 66-67 1.4160428974997146 1106 68-69 2.243703019721046 1106 70-71 3.388750193265338 1106 72-73 2.967812519024619 1106 74-75 3.5710102135581465 1106 76-77 3.530623724247935 1106 78-79 3.9990992731115362 1106 80-81 4.157099973618259 1106 82-83 3.7801126481420546 1106 84-85 3.654961754541585 1106 86-87 2.9469311906484315 1106 88-89 2.963712289027054 1106 90-91 3.345810702115042 1106 92-93 4.129651633387546 1106 94-95 3.014276454196132 1106 96-97 3.626772808262011 1106 98-99 3.493589810510816 1106 100-101 2.4869411847857172 1107 1 -0.3415187813405076 1107 2 -0.5623484589136254 1107 3 -0.14476999210553032 1107 4 -0.09607932987977819 1107 5 0.36835756476593673 1107 6 0.32332672585111055 1107 7 -0.2582321622016792 1107 8 -0.2975897932673419 1107 9 0.3599753793498053 1107 10-11 0.39622656142781665 1107 12-13 0.09809217265725323 1107 14-15 -0.1343854365488042 1107 16-17 -0.12394962687336886 1107 18-19 -0.2671087226553084 1107 20-21 0.21608569715844794 1107 22-23 0.10714353380709696 1107 24-25 0.7836845166772406 1107 26-27 -0.007363640434405738 1107 28-29 0.34926939908904586 1107 30-31 0.6903976504951714 1107 32-33 0.9092210247124939 1107 34-35 0.9665034449537657 1107 36-37 0.7503939887946629 1107 38-39 -0.22662043301555457 1107 40-41 0.6370828432135553 1107 42-43 0.524831795639642 1107 44-45 0.7601375714091674 1107 46-47 1.4122230779993998 1107 48-49 1.2304850914590801 1107 50-51 0.155048412592393 1107 52-53 -0.16924457267706572 1107 54-55 0.22492229862542246 1107 56-57 -0.16245134430384667 1107 58-59 -0.2790562558213452 1107 60-61 -0.27227774062014376 1107 62-63 -0.8833809534776336 1107 64-65 -0.39626404415153615 1107 66-67 0.07854289749971599 1107 68-69 -1.1392515257334992 1107 70-71 -1.7589770794619355 1107 72-73 -1.979914753702655 1107 74-75 -2.084671604623672 1107 76-77 -1.636421730297517 1107 78-79 -2.1156734541611932 1107 80-81 -2.445172753654468 1107 82-83 -3.1130691700397684 1107 84-85 -2.6314018818220504 1107 86-87 -2.5689779002606628 1107 88-89 -2.445378620063856 1107 90-91 -3.1303256615213186 1107 92-93 -2.2805756393397267 1107 94-95 -2.5675417276220465 1107 96-97 -1.3482271917379904 1107 98-99 -1.3541374622164604 1107 100-101 -0.7948769970324641 1108 1 0.08840546108373815 1108 2 0.10053032896516356 1108 3 -0.1523457496812881 1108 4 -0.008958117758567141 1108 5 0.08426665567503022 1108 6 -0.00432478930040503 1108 7 0.4292678377983208 1108 8 0.47513747945993146 1108 9 0.6667935611679852 1108 10-11 -0.30926586281460544 1108 12-13 0.21267550599058183 1108 14-15 0.1497054725421023 1108 16-17 -0.6050102329339779 1108 18-19 -0.13263902568561292 1108 20-21 0.2804796365523856 1108 22-23 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94-95 2.0344892201535814 2312 96-97 2.348581318900308 2312 98-99 2.2802919381703894 2312 100-101 1.0943879932963547 2313 1 -0.33168160359288024 2313 2 -0.45108658645772337 2313 3 -0.5341865457011927 2313 4 -0.22972926203717492 2313 5 -0.13028558313094152 2313 6 -0.48162578432528136 2313 7 -0.5753963413061598 2313 8 -0.3830714757639484 2313 9 -0.6186542000260431 2313 10-11 -0.6797571563469447 2313 12-13 -0.9373555885367821 2313 14-15 -1.637607960293721 2313 16-17 -1.2306321234812359 2313 18-19 -0.9967559411084963 2313 20-21 -0.5895452390695084 2313 22-23 -0.856051852895753 2313 24-25 -0.4371431631056595 2313 26-27 -1.4329063812756573 2313 28-29 -1.1451335859855831 2313 30-31 -0.755328265379994 2313 32-33 -0.8411181883132883 2313 34-35 -0.5579198929024116 2313 36-37 -1.0760985485187646 2313 38-39 -1.275127895702127 2313 40-41 -0.6875440224580913 2313 42-43 -1.0528479872640162 2313 44-45 -2.0982409903276036 2313 46-47 -1.7578922544293647 2313 48-49 -1.1418012043346764 2313 50-51 0.009017204994023587 2313 52-53 0.4489711668073326 2313 54-55 0.9589792864137578 2313 56-57 0.6019652092918122 2313 58-59 0.9633114510985941 2313 60-61 0.544377618945667 2313 62-63 0.8415172826146353 2313 64-65 0.8812122109366598 2313 66-67 0.18861752436538737 2313 68-69 0.6935164525568709 2313 70-71 0.8383770589369774 2313 72-73 2.080125951860442 2313 74-75 2.229778870274565 2313 76-77 2.3031983511136076 2313 78-79 3.191823153708551 2313 80-81 2.547025346752587 2313 82-83 1.9495156332166808 2313 84-85 2.478842351556512 2313 86-87 1.5275282055738018 2313 88-89 1.3890854233554109 2313 90-91 0.7892808513687761 2313 92-93 0.7626740214472498 2313 94-95 0.8493510810618083 2313 96-97 1.3346086291575325 2313 98-99 1.6126196612570816 2313 100-101 -0.33320806894562693 2314 1 -0.14346683328855647 2314 2 0.02206712550195533 2314 3 0.12795728062174305 2314 4 -0.19348192728237734 2314 5 -0.18359048718211568 2314 6 -0.095504442980058 2314 7 -0.46277761674713247 2314 8 -0.336550832228383 2314 9 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1.8220332982453975 2314 74-75 1.100393330441264 2314 76-77 0.6557860619102733 2314 78-79 0.11322264973491158 2314 80-81 -0.07828963677135192 2314 82-83 0.1093334273628308 2314 84-85 -0.017765518185687057 2314 86-87 -0.02189997818273781 2314 88-89 0.061114886429653126 2314 90-91 0.47032368912803335 2314 92-93 0.6528659191018313 2314 94-95 0.40973099965068016 2314 96-97 1.1615130680022716 2314 98-99 1.5419664338874348 2314 100-101 2.072006119850652 2315 1 -0.6092571405422795 2315 2 -0.29072983363646543 2315 3 -0.4308010342341362 2315 4 -0.3311125893032809 2315 5 -0.8212211492030193 2315 6 -0.6450768218207301 2315 7 -0.5204272841529018 2315 8 -0.7077893498083583 2315 9 -0.6228405851734777 2315 10-11 -0.49346301728615316 2315 12-13 -1.075688315147623 2315 14-15 -1.0759042835554595 2315 16-17 -0.850945192283568 2315 18-19 -1.3059113834091889 2315 20-21 -0.8770406886508724 2315 22-23 -1.0306059118691735 2315 24-25 -1.6520139312163309 2315 26-27 -1.3358004475297562 2315 28-29 -1.4891452350572933 2315 30-31 -1.539092371677775 2315 32-33 -0.9732623455757548 2315 34-35 -1.3222992158001219 2315 36-37 -1.3563133282785103 2315 38-39 -1.3119862866740917 2315 40-41 -1.9238927665425436 2315 42-43 -1.9967868296375144 2315 44-45 -2.7817138698325223 2315 46-47 -2.0891627313132375 2315 48-49 -1.840745507210535 2315 50-51 -1.013188837961927 2315 52-53 -1.248790027222519 2315 54-55 -1.4591131780929771 2315 56-57 -1.568493472906951 2315 58-59 -0.4672491604776674 2315 60-61 -0.696612552150075 2315 62-63 -0.9249552328567887 2315 64-65 -0.750476904461955 2315 66-67 -1.6988961268905278 2315 68-69 -0.5937969802789524 2315 70-71 -1.888688831124906 2315 72-73 -1.8535289443900176 2315 74-75 -1.1689288108320959 2315 76-77 -1.0538274952642581 2315 78-79 -1.8487665805470037 2315 80-81 -1.498387831259791 2315 82-83 -2.1698873518579482 2315 84-85 -2.9279650747267034 2315 86-87 -2.3530688093515693 2315 88-89 -2.2192145402412358 2315 90-91 -2.1142502734947115 2315 92-93 -1.9560190983197714 2315 94-95 -2.6540162287306934 2315 96-97 -1.9884710941770152 2315 98-99 -1.7216540919282117 2315 100-101 -0.9301319859459909 2316 1 -0.7151381526234282 2316 2 -0.24798992025602118 2316 3 -0.11418375591181729 2316 4 -0.4618397376962591 2316 5 -0.59213521348385 2316 6 -0.42624846531286664 2316 7 -0.27926019958485426 2316 8 -0.22112420278305933 2316 9 -0.2969914855609872 2316 10-11 -0.732065707051369 2316 12-13 -0.6717676404268573 2316 14-15 -0.6610421910092938 2316 16-17 -1.2927049369838173 2316 18-19 -0.8637527328507204 2316 20-21 -0.7798786188993319 2316 22-23 -1.060124945377268 2316 24-25 -0.45102236522591 2316 26-27 -0.5257366225924329 2316 28-29 -0.9696558345558159 2316 30-31 -0.9863228252907277 2316 32-33 -0.3074314816596413 2316 34-35 -1.1558414998210864 2316 36-37 -0.7390919925137425 2316 38-39 -0.5981157601183611 2316 40-41 -0.701702203515417 2316 42-43 -1.847937957971233 2316 44-45 -1.010571859347003 2316 46-47 -1.090644381643763 2316 48-49 -1.6309210257881546 2316 50-51 -1.4911920292087935 2316 52-53 -0.5408461019888726 2316 54-55 -0.6166240055377017 2316 56-57 -0.7385983281611104 2316 58-59 0.09275767790847667 2316 60-61 0.21699583627026087 2316 62-63 1.2502214254518478 2316 64-65 0.017074953299612616 2316 66-67 -0.14692439221990838 2316 68-69 0.6748946085060936 2316 70-71 0.13290907177000832 2316 72-73 0.06150410780966453 2316 74-75 0.6984634845861848 2316 76-77 0.3461611074255053 2316 78-79 0.3347301142330288 2316 80-81 -6.10306755575607E-4 2316 82-83 0.15721545188037211 2316 84-85 0.6054290442612107 2316 86-87 0.8151554897138524 2316 88-89 0.42165621426069677 2316 90-91 1.058077057255229 2316 92-93 0.21761892310717101 2316 94-95 0.6072671084017394 2316 96-97 0.4765391634021938 2316 98-99 0.6241972871463268 2316 100-101 0.8051654838511375 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 0.0 5 3.0 6 13.0 7 17.0 8 48.0 9 89.0 10 192.0 11 294.0 12 442.0 13 618.0 14 817.0 15 1259.0 16 1744.0 17 2533.0 18 2235.0 19 1258.0 20 1027.0 21 1013.0 22 1073.0 23 1128.0 24 1146.0 25 1247.0 26 1368.0 27 1526.0 28 1751.0 29 2038.0 30 2437.0 31 2874.0 32 3594.0 33 4769.0 34 6678.0 35 10513.0 36 14476.0 37 20643.0 38 15728.0 39 1259.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8930979162996 21.4564574311229 16.829103183508444 17.821341469069058 2 15.605789468804184 24.782338596767765 38.92407117226544 20.687800762162613 3 18.728036421292735 29.708203135865887 32.2645550723697 19.29920537047168 4 11.807957274387814 20.702636044172053 38.99546596693587 28.49394071450427 5 11.516814864952574 40.20361424557955 33.65383723841225 14.625733651055622 6 26.421637258810765 40.80629757721301 17.399004181695116 15.373060982281109 7 23.69472698445077 38.298207712492236 21.99608719437001 16.010978108686984 8 25.588079850905416 33.67979898192877 20.191746019971998 20.540375147193814 9 27.047500718583972 18.420784230095226 21.525066990570323 33.00664806075048 10-11 23.199707001821967 29.4611522431514 26.777344564930157 20.561796190096477 12-13 26.81118770891187 28.163522654044254 27.291945795337064 17.73334384170681 14-15 22.80692807669841 28.713688329269083 29.248222084171683 19.231161509860826 16-17 18.501914678584345 32.05116317883005 29.242658853418142 20.20426328916746 18-19 18.147258718046196 29.35948669924247 34.63621106897479 17.857043513736546 20-21 19.94427342185298 27.54571248423707 34.84626511386396 17.66374898004599 22-23 19.533430380803146 28.07159877979806 34.658463991988945 17.736506847409853 24-25 19.659423347071996 28.292063256573 35.16943062593594 16.879082770419068 26-27 19.168270010895014 28.423004705718725 35.51079069983078 16.897934583555482 28-29 17.998896569629704 29.277067656972243 35.64033733442749 17.083698438970565 30-31 18.696472704846958 28.96258479743672 34.682209578922475 17.65873291879385 32-33 18.429007223061873 29.68177729974316 34.31139834398094 17.57781713321403 34-35 18.801259197099583 28.841918838348953 34.80363294126374 17.55318902328773 36-37 19.199135804758637 28.922723142258405 34.34712460360143 17.531016449381525 38-39 18.331648879451826 29.741583139389334 34.718448942502945 17.208319038655898 40-41 17.926024145540865 30.187953192515256 34.26599039370955 17.620032268234333 42-43 18.358708552777983 30.225409836065577 33.192085156887465 18.22379645426897 44-45 18.875840870843167 29.67375833916708 32.81563660471305 18.634764185276705 46-47 19.39852203502981 30.373849106637863 32.03354628144384 18.194082576888484 48-49 19.638295603636514 30.58307564638087 31.407828429168156 18.37080032081446 50-51 18.814846408470945 30.812417131041904 31.69094398753836 18.681792472948793 52-53 18.977083594115822 31.509942187420314 30.633713032633743 18.879261185830124 54-55 18.6517135227357 31.014019731473923 30.609747051405684 19.72451969438469 56-57 19.40055632823366 30.882243857209087 30.258229021789525 19.458970792767733 58-59 19.63152355829188 30.08034344155513 30.57130538389144 19.71682761626155 60-61 18.844315458115243 30.793837709029713 31.460043857412412 18.90180297544263 62-63 18.727689126665492 30.201388953278137 31.712269705426934 19.358652214629444 64-65 18.522399061664064 30.53068831391602 31.58677984803038 19.360132776389538 66-67 18.690600921641895 30.18052683795236 30.69095325958962 20.437918980816125 68-69 18.712390242093257 30.150856273933478 30.547700067685973 20.589053416287285 70-71 19.033203215547374 29.997867428211144 30.6190948623564 20.349834493885083 72-73 18.45509081974205 29.42068223753141 31.32747957830711 20.796747364419428 74-75 18.192776111376396 30.503799240615482 30.284977816514523 21.018446831493595 76-77 18.426981919332405 29.7185906351414 30.19425127491887 21.660176170607325 78-79 18.334175548560275 29.76574053898684 29.896940672504996 22.003143239947892 80-81 18.515049795070563 29.89651527234287 29.13475269375568 22.453682238830883 82-83 18.20006768754317 28.953578399328688 29.618863498333308 23.227490414794826 84-85 17.60830458810875 28.743833685694153 29.70679870924669 23.94106301695041 86-87 17.717146897239157 29.429518533113885 29.1124040891073 23.740930480539653 88-89 17.42351539427994 28.68978242627389 29.00272141012624 24.883980769319926 90-91 17.29523738879282 28.528379562449874 29.506585504935074 24.669797543822234 92-93 17.354498335666797 29.209279468896902 28.832370585344595 24.603851610091702 94-95 17.310072739579326 29.510757947343773 28.169549515301274 25.009619797775624 96-97 17.09566152676427 30.035976226019713 27.808788213149864 25.059574034066145 98-99 17.376710201167413 30.354998585940073 26.406757815578768 25.861533397313753 100-101 16.463683188146444 29.106764519075192 26.73957691041683 27.68997538236153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 817.0 1 562.0 2 299.5 3 282.5 4 269.0 5 290.0 6 383.5 7 641.0 8 900.5 9 952.5 10 816.5 11 646.5 12 627.0 13 659.5 14 620.0 15 575.5 16 550.0 17 529.0 18 529.5 19 503.0 20 521.5 21 560.0 22 563.5 23 600.5 24 668.0 25 764.0 26 871.0 27 1000.0 28 1082.5 29 1220.0 30 1495.0 31 1695.5 32 1955.0 33 2294.0 34 2559.5 35 2864.0 36 3376.5 37 3696.0 38 3801.5 39 3792.5 40 3531.0 41 3288.0 42 3094.0 43 3122.5 44 3261.5 45 3258.0 46 3186.5 47 3722.5 48 4966.0 49 4681.5 50 3344.0 51 2656.0 52 2269.0 53 2139.0 54 1972.5 55 1848.5 56 1814.5 57 1655.0 58 1502.5 59 1319.5 60 1096.0 61 929.5 62 754.5 63 579.0 64 394.0 65 282.5 66 223.5 67 189.5 68 129.5 69 61.0 70 37.0 71 33.0 72 26.5 73 12.5 74 11.0 75 12.0 76 7.5 77 4.0 78 3.5 79 1.0 80 1.0 81 2.0 82 1.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012980871758259079 2 0.0 3 0.0018544102511798685 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.636025627949671E-4 12-13 4.636025627949671E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0027816153767698027 22-23 0.0 24-25 0.004172423065154704 26-27 0.0032452179395647696 28-29 0.005099628190744639 30-31 0.019007705074593654 32-33 0.0018544102511798685 34-35 0.004172423065154704 36-37 0.004636025627949672 38-39 0.0018544102511798685 40-41 0.004636025627949672 42-43 0.0027816153767698027 44-45 0.0013908076883849014 46-47 0.0 48-49 4.636025627949671E-4 50-51 0.0 52-53 0.002318012813974836 54-55 0.0027816153767698027 56-57 9.272051255899342E-4 58-59 0.0013908076883849014 60-61 0.0013908076883849014 62-63 0.0 64-65 4.636025627949671E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.636025627949671E-4 76-77 9.272051255899342E-4 78-79 4.636025627949671E-4 80-81 0.008344846130309409 82-83 0.002318012813974836 84-85 0.006490435879129539 86-87 0.0032452179395647696 88-89 0.002318012813974836 90-91 4.636025627949671E-4 92-93 0.0 94-95 4.636025627949671E-4 96-97 0.0018544102511798685 98-99 0.005099628190744639 100-101 0.0013908076883849014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 107851.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.09820029485123 #Duplication Level Percentage of deduplicated Percentage of total 1 84.00953905048793 49.64812565483862 2 8.855000156892277 10.466291457659178 3 2.6954093319526815 4.778815217290521 4 1.273965295428159 3.0115622479161064 5 0.7593586243685085 2.243836403927641 6 0.465970064953403 1.6522795338012628 7 0.3043710188584518 1.2591445605511307 8 0.2824060999717594 1.3351753808495053 9 0.1631679688725721 0.8678639975521785 >10 1.0794188710031694 11.043013045776116 >50 0.06746367943769808 2.6527338643128022 >100 0.034516301107659485 3.7839241175325213 >500 0.003137845555241771 1.6643332004339322 >1k 0.006275691110483542 5.592901317558484 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1806 1.6745324568154214 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1631 1.5122715598371828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1498 1.3889532781337215 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1097 1.0171440227721578 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 990 0.9179330743340349 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 805 0.7464001260998971 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 325 0.30134166581672867 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 324 0.3004144606911387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 306 0.28372476843051986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.25312699928605203 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 231 0.21418438401127482 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 219 0.20305792250419563 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.18544102511798685 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 177 0.16411530722941836 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 176 0.16318810210382845 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 173 0.16040648672705862 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 173 0.16040648672705862 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 170 0.15762487135028883 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 167 0.15484325597351903 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 162 0.15020723034556935 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 161 0.1492800252199794 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 148 0.13722635858731028 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.13166312783377065 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 120 0.1112646150707921 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 116 0.10755579456843238 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 108 0.1001381535637129 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 9.272051255899342E-4 0.0 22-23 0.0 0.0 0.0 0.0018544102511798685 0.0 24-25 0.0 0.0 0.0 0.004636025627949672 0.0 26-27 0.0 0.0 0.0 0.009272051255899343 0.0 28-29 0.0 0.0 0.0 0.023643730702543324 0.0 30-31 0.0 0.0 0.0 0.04636025627949671 0.0 32-33 0.0 0.0 0.0 0.06675876904247527 0.0 34-35 0.0 0.0 0.0 0.09040249974501859 0.0 36-37 0.0 0.0 0.0 0.11080101250799715 0.0 38-39 0.0 0.0 0.0 0.1539160508479291 0.0 40-41 0.0 0.0 0.0 0.2280924608951238 0.0 42-43 0.0 0.0 0.0 0.3727364604871536 0.0 44-45 0.0 0.0 0.0 0.49420033193943497 0.0 46-47 0.0 0.0 0.0 0.8210401387098868 0.0 48-49 0.0 0.0 0.0 1.1348990737220794 0.0 50-51 0.0 0.0 0.0 1.3064320219562173 0.0 52-53 0.0 0.0 0.0 1.5308156623489815 0.0 54-55 0.0 0.0 0.0 1.7408276232951017 0.0 56-57 0.0 0.0 0.0 2.2345643526717414 0.0 58-59 0.0 0.0 0.0 2.8289028381748897 0.0 60-61 0.0 0.0 0.0 3.3963523750359292 0.0 62-63 0.0 0.0 0.0 4.024533847623109 0.0 64-65 0.0 0.0 0.0 4.752389871211208 0.0 66-67 0.0 0.0 0.0 5.639261573837981 0.0 68-69 0.0 0.0 0.0 6.499707930385439 0.0 70-71 0.0 0.0 0.0 7.241008428294592 0.0 72-73 0.0 0.0 0.0 7.947075131431327 0.0 74-75 0.0 0.0 0.0 8.688839231903273 0.0 76-77 0.0 0.0 0.0 9.603527088297744 0.0 78-79 0.0 0.0 0.0 10.382842996356084 0.0 80-81 0.0 0.0 0.0 11.328128621895022 0.0 82-83 0.0 0.0 0.0 12.516805592901317 0.0 84-85 0.0 0.0 0.0 14.242334331624185 0.0 86-87 0.0 0.0 0.0 15.761096327340496 0.0 88-89 0.0 0.0 0.0 16.94096484965369 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATA 25 5.254698E-5 75.99703 6 GTATGGT 20 0.0020654837 71.280266 1 TAAGGTG 40 8.985808E-8 71.24722 5 GGGATGC 20 0.0020692889 71.24722 7 GGTGTGC 45 2.0307016E-7 63.33086 8 GTCCAAC 25 0.0050005605 57.02421 1 AGGTAAG 25 0.0050097536 56.997776 2 CGGCCTT 25 0.0050097536 56.997776 5 GTAAACC 25 0.0050097536 56.997776 4 AAGGTGT 35 2.7771376E-4 54.283592 6 TGAACGC 20 5.3794263E-4 47.49815 76-77 GTAAGGT 40 5.3673907E-4 47.49815 4 GGTAAGG 45 9.58811E-4 42.220573 3 TGTAGAT 45 9.58811E-4 42.220573 5 GTTGAGC 25 0.0016132161 37.998516 68-69 ATAGATT 50 0.0016096388 37.998516 4 TTGAACG 25 0.0016132161 37.998516 74-75 ACGCTTT 25 0.0016132161 37.998516 78-79 TAGATAT 50 0.0016096388 37.998516 7 AGGTGTG 50 0.0016096388 37.998516 7 >>END_MODULE