##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936930_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68153 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.31945769078397 33.0 31.0 34.0 31.0 34.0 2 32.37383534107082 33.0 31.0 34.0 31.0 34.0 3 32.44337006441389 34.0 31.0 34.0 31.0 34.0 4 36.13901075521254 37.0 35.0 37.0 35.0 37.0 5 36.08833066776224 37.0 35.0 37.0 35.0 37.0 6 36.177072175839655 37.0 36.0 37.0 35.0 37.0 7 36.151159890246944 37.0 36.0 37.0 35.0 37.0 8 36.149971387906625 37.0 36.0 37.0 35.0 37.0 9 37.848869455489854 39.0 38.0 39.0 35.0 39.0 10-11 37.75414141710563 39.0 37.0 39.0 35.0 39.0 12-13 37.68102651387319 39.0 37.0 39.0 35.0 39.0 14-15 38.76757442812496 40.0 38.0 41.0 35.0 41.0 16-17 38.746966384458496 40.0 38.0 41.0 35.0 41.0 18-19 38.80349360996581 40.0 38.0 41.0 35.0 41.0 20-21 38.8912226901237 40.0 38.0 41.0 35.0 41.0 22-23 38.80299473244025 40.0 38.0 41.0 35.0 41.0 24-25 38.66855457573401 40.0 38.0 41.0 35.0 41.0 26-27 38.4202676331196 40.0 38.0 41.0 34.5 41.0 28-29 38.19836250788667 40.0 37.0 41.0 34.0 41.0 30-31 37.923055478115415 40.0 37.0 41.0 34.0 41.0 32-33 37.595630419790766 40.0 35.0 41.0 33.5 41.0 34-35 37.331386732792396 40.0 35.0 41.0 33.0 41.0 36-37 37.017893563012635 39.0 35.0 41.0 33.0 41.0 38-39 36.73662201223718 39.0 35.0 41.0 33.0 41.0 40-41 36.3405572755418 39.0 35.0 41.0 31.5 41.0 42-43 35.906218361627516 39.0 35.0 41.0 30.5 41.0 44-45 35.33209836690975 39.0 35.0 41.0 28.5 41.0 46-47 34.506793538068756 38.0 35.0 40.5 22.0 41.0 48-49 33.72779628189515 38.0 34.5 40.0 8.5 41.0 50-51 33.3112702302173 38.0 34.0 40.0 2.0 41.0 52-53 32.99743958446437 38.0 33.5 40.0 2.0 41.0 54-55 32.65300867166522 38.0 33.0 40.0 2.0 41.0 56-57 32.26810265138732 37.0 33.0 40.0 2.0 41.0 58-59 31.860703123853682 37.0 33.0 40.0 2.0 41.0 60-61 31.411280501225185 36.0 32.0 40.0 2.0 41.0 62-63 30.973948322157497 36.0 31.0 39.0 2.0 41.0 64-65 30.493617302246417 35.5 31.0 39.0 2.0 40.5 66-67 29.943905624110457 35.0 29.5 39.0 2.0 40.0 68-69 29.385470925711267 35.0 29.0 38.0 2.0 40.0 70-71 28.911236482619987 35.0 28.0 37.5 2.0 40.0 72-73 28.497402902293366 35.0 27.5 37.0 2.0 39.0 74-75 28.03208222675451 34.5 26.0 36.5 2.0 39.0 76-77 26.665546637712204 32.5 25.0 35.0 2.0 37.5 78-79 27.20412160873329 34.0 25.5 35.5 2.0 37.5 80-81 26.714898830572388 34.0 23.0 35.0 2.0 37.0 82-83 26.567267765175416 34.0 24.0 35.0 2.0 37.0 84-85 26.22348979502003 34.0 22.0 35.0 2.0 36.0 86-87 25.867614044869633 34.0 20.0 35.0 2.0 36.0 88-89 25.49906827285666 33.0 18.0 35.0 2.0 35.5 90-91 25.07454550790134 33.0 9.5 35.0 2.0 35.0 92-93 24.727348759408976 33.0 3.5 35.0 2.0 35.0 94-95 24.307910143353922 33.0 2.0 35.0 2.0 35.0 96-97 23.753481138027674 32.0 2.0 34.5 2.0 35.0 98-99 23.129810866726338 32.0 2.0 34.0 2.0 35.0 100-101 20.705339456810414 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.44262343643250546 1101 2 0.45653633022524787 1101 3 0.2780179291863405 1101 4 0.24421893681156348 1101 5 0.24472536381884424 1101 6 0.2429929110036113 1101 7 0.3512087244004576 1101 8 0.2985646083761253 1101 9 0.42017908899347844 1101 10-11 0.374554593500811 1101 12-13 0.22696290553817278 1101 14-15 0.4342627915223929 1101 16-17 0.501670587449496 1101 18-19 0.4804727026630218 1101 20-21 0.14481998154276 1101 22-23 0.37387882518297744 1101 24-25 0.555507039126546 1101 26-27 0.7077895626367976 1101 28-29 0.4907852083176536 1101 30-31 0.7973582888480948 1101 32-33 1.0979711331237496 1101 34-35 1.2685728775150125 1101 36-37 1.4406052699263157 1101 38-39 1.5760953626833256 1101 40-41 1.5754820588229919 1101 42-43 1.8321549656897034 1101 44-45 2.2479824858915407 1101 46-47 2.0643075807879327 1101 48-49 2.589513319316133 1101 50-51 2.953090427724625 1101 52-53 3.1671681278162858 1101 54-55 3.3302101916002194 1101 56-57 3.5704534141928725 1101 58-59 3.5060105620771687 1101 60-61 3.353643762960374 1101 62-63 3.4541233852247437 1101 64-65 3.882905770523223 1101 66-67 3.7980918953848253 1101 68-69 3.664415243681642 1101 70-71 3.967357754127118 1101 72-73 4.137022985469997 1101 74-75 4.051590600281809 1101 76-77 4.0405418384119045 1101 78-79 4.328714256382678 1101 80-81 4.08336227473551 1101 82-83 4.00433822075993 1101 84-85 3.333985272160991 1101 86-87 3.5803757282778363 1101 88-89 3.4641617045087756 1101 90-91 3.1759009187807443 1101 92-93 2.89453415072774 1101 94-95 2.898909939884309 1101 96-97 3.1063329611995876 1101 98-99 2.640575699712265 1101 100-101 2.8645256860077417 1102 1 0.17141471320924495 1102 2 0.1857529414695449 1102 3 0.1251681882536957 1102 4 0.019371670552189357 1102 5 0.09137295487908403 1102 6 0.16554337113817041 1102 7 0.07663599220650497 1102 8 0.1780016202081569 1102 9 0.0671901476896366 1102 10-11 -0.06572458057698327 1102 12-13 0.25834098921305326 1102 14-15 0.11961195245348932 1102 16-17 0.2742906477696536 1102 18-19 0.22283601563186295 1102 20-21 0.08573715732190124 1102 22-23 -0.10340444757240874 1102 24-25 -4.437767422942329E-4 1102 26-27 0.037694442383148896 1102 28-29 -0.14333048265751813 1102 30-31 0.3036803057068056 1102 32-33 -0.021760519493994934 1102 34-35 -0.24700983070554372 1102 36-37 -0.008605927970215532 1102 38-39 0.275693228277639 1102 40-41 0.7115310882870745 1102 42-43 0.9000538134199658 1102 44-45 1.412722258530934 1102 46-47 1.738346711558954 1102 48-49 1.3769620858461806 1102 50-51 1.4431530678916644 1102 52-53 1.3061558317834887 1102 54-55 0.8708876334067277 1102 56-57 1.4113589899410748 1102 58-59 1.6724710059716905 1102 60-61 2.1236924830902986 1102 62-63 1.431909325987256 1102 64-65 1.5312701661616117 1102 66-67 2.027269839859347 1102 68-69 2.1495672040868676 1102 70-71 1.8285053838541288 1102 72-73 2.256309970235293 1102 74-75 2.357831416923986 1102 76-77 2.3429933116158317 1102 78-79 2.348994977131266 1102 80-81 2.485670680891257 1102 82-83 2.6185405252994087 1102 84-85 2.339669287318369 1102 86-87 2.3421497596752516 1102 88-89 2.5029831413659416 1102 90-91 2.8335646725507573 1102 92-93 2.722467025215405 1102 94-95 2.5470230015191397 1102 96-97 2.346124933978178 1102 98-99 2.4348878178779145 1102 100-101 1.8071635330420044 1103 1 -0.11396561407001826 1103 2 -0.06752884238228063 1103 3 -0.037482414155945776 1103 4 -0.2491951339793701 1103 5 -0.11578403127448667 1103 6 0.05234430622179076 1103 7 0.03464714128041635 1103 8 0.13538338607760636 1103 9 -0.32743312150655157 1103 10-11 -0.09822772749301123 1103 12-13 -0.15296093490130858 1103 14-15 -0.15996905815611484 1103 16-17 -0.7379823247173007 1103 18-19 -0.2765346011636822 1103 20-21 0.14229173383691318 1103 22-23 0.2763384044326145 1103 24-25 0.5285078506628551 1103 26-27 0.409300268673384 1103 28-29 0.6395772677470788 1103 30-31 0.6493465527846709 1103 32-33 -0.02135591600540465 1103 34-35 0.13835251419645545 1103 36-37 0.44747211554714994 1103 38-39 0.017196555017427784 1103 40-41 0.5279759723007373 1103 42-43 0.9567882137076822 1103 44-45 1.1095843336972706 1103 46-47 1.7164082112892132 1103 48-49 0.46075996392566054 1103 50-51 0.8246492016805504 1103 52-53 1.142291418908119 1103 54-55 1.7588574230129161 1103 56-57 1.2708716675170528 1103 58-59 1.2297448775775166 1103 60-61 1.2651238803210134 1103 62-63 1.5725764721839859 1103 64-65 1.895952777202794 1103 66-67 1.4726034129577066 1103 68-69 2.056418489826843 1103 70-71 2.38155339680144 1103 72-73 2.642391610228099 1103 74-75 2.0470959376936335 1103 76-77 1.9062013677208505 1103 78-79 2.5969270037451864 1103 80-81 3.612671220362575 1103 82-83 3.3876018452059 1103 84-85 2.848031092749043 1103 86-87 2.3969060984632407 1103 88-89 1.8040081368703476 1103 90-91 2.178241888878752 1103 92-93 1.3921307547604513 1103 94-95 1.606319890567871 1103 96-97 1.8679285664183887 1103 98-99 1.975303210792859 1103 100-101 1.2120817132254231 1104 1 0.23498574692953156 1104 2 0.2711592432937593 1104 3 0.1251681882536957 1104 4 0.2592832238965812 1104 5 0.16489423735558972 1104 6 0.007721370032584218 1104 7 0.03821697617554776 1104 8 0.04256767880406187 1104 9 0.3684615683640402 1104 10-11 0.19795701146281885 1104 12-13 0.22945762824014082 1104 14-15 -0.18083028082456565 1104 16-17 -0.4878707673768261 1104 18-19 0.042073163227215105 1104 20-21 0.2899936526231741 1104 22-23 0.3809791897962995 1104 24-25 0.2863168644959657 1104 26-27 0.8114644810785521 1104 28-29 0.21487847256636172 1104 30-31 -0.36671770424344885 1104 32-33 -0.3443144166747558 1104 34-35 -0.8659982220819984 1104 36-37 0.07431281166494585 1104 38-39 0.5493250690736602 1104 40-41 0.7101491092931553 1104 42-43 0.10292832972731247 1104 44-45 -0.24371776358073305 1104 46-47 -0.5745333326643802 1104 48-49 -0.34111419939428345 1104 50-51 0.27980315081040175 1104 52-53 0.042336041844301064 1104 54-55 0.7874161021740029 1104 56-57 0.8845485974590375 1104 58-59 1.676755140852837 1104 60-61 1.7312104488172224 1104 62-63 1.6240044061420384 1104 64-65 0.9045426924192128 1104 66-67 0.5307247873441447 1104 68-69 -0.2734565659518289 1104 70-71 0.7078586176849733 1104 72-73 0.8632751443646427 1104 74-75 0.6255207480461529 1104 76-77 1.0887091767346853 1104 78-79 1.3648877355613394 1104 80-81 1.3753887571764984 1104 82-83 0.9905692704624833 1104 84-85 0.6087405974344513 1104 86-87 1.47689271158238 1104 88-89 1.5043651203598607 1104 90-91 1.8096564359559508 1104 92-93 1.6963476222303306 1104 94-95 1.642241354200177 1104 96-97 2.136893314298799 1104 98-99 0.8974361871427021 1104 100-101 1.2739960371879384 1105 1 0.0347542654480506 1105 2 0.34060368773820215 1105 3 1.6818825369568913E-4 1105 4 0.21993137204472646 1105 5 0.32924608920744447 1105 6 0.23457322188443896 1105 7 -0.1099311719725975 1105 8 0.20691953065591662 1105 9 0.551331938734414 1105 10-11 0.5625403447961475 1105 12-13 -0.23466274213022587 1105 14-15 0.01303545991617483 1105 16-17 0.3981246029935406 1105 18-19 -0.21139905899500633 1105 20-21 0.4416140229935408 1105 22-23 0.0696365972037043 1105 24-25 0.33724279042188954 1105 26-27 0.24664966626374252 1105 28-29 0.8555034725663617 1105 30-31 0.5655739624232226 1105 32-33 0.7303383611030227 1105 34-35 0.6270573334735587 1105 36-37 1.4510489227760601 1105 38-39 1.9659917357403245 1105 40-41 2.618713924107965 1105 42-43 2.272488514912503 1105 44-45 1.621444273456305 1105 46-47 2.796415741409696 1105 48-49 1.4991635783834951 1105 50-51 2.265914261921516 1105 52-53 1.9092341899924499 1105 54-55 1.3678559169888196 1105 56-57 2.104456004866446 1105 58-59 2.110204214926913 1105 60-61 0.8104928562246272 1105 62-63 2.036620146882779 1105 64-65 2.579311210937732 1105 66-67 2.6186877503071067 1105 68-69 3.447608248862988 1105 70-71 2.447441951018309 1105 72-73 2.3997334776979784 1105 74-75 2.995891118416523 1105 76-77 2.452135102660609 1105 78-79 3.025767365190969 1105 80-81 2.30073597939872 1105 82-83 3.7799211223143345 1105 84-85 3.6330461529900084 1105 86-87 3.391823267137937 1105 88-89 2.814550305545044 1105 90-91 2.6684527322522484 1105 92-93 2.8369726222303306 1105 94-95 1.9483756134594366 1105 96-97 3.006106277261761 1105 98-99 1.738292668624183 1105 100-101 0.6108015927434955 1106 1 -0.2464957345519494 1106 2 -0.2573692852347733 1106 3 0.0035465666320746436 1106 4 0.09662056123391238 1106 5 0.2870163594777182 1106 6 0.3190326813438986 1106 7 0.2143931523517253 1106 8 -0.2136885774521886 1106 9 -0.22062752072504566 1106 10-11 0.18669575020155094 1106 12-13 -0.005777606995096107 1106 14-15 -0.2171165671108568 1106 16-17 0.19331041380434755 1106 18-19 0.31985094100499367 1106 20-21 0.14685050948002498 1106 22-23 0.31541362423073593 1106 24-25 0.043323871502970235 1106 26-27 -0.14524222562814515 1106 28-29 -0.11620260851471897 1106 30-31 0.01616517863943301 1106 32-33 0.0618417394814017 1106 34-35 -0.07395618003995708 1106 36-37 -1.0240355366833995 1106 38-39 -0.16238664263805447 1106 40-41 0.5857747349187719 1106 42-43 -0.19837297157398126 1106 44-45 -0.9026266724896388 1106 46-47 -1.6761349342659813 1106 48-49 -1.7254985837786663 1106 50-51 -1.582058711051456 1106 52-53 -1.8230293235210624 1106 54-55 -3.1545258397679383 1106 56-57 -2.687773724863284 1106 58-59 -2.418089703992006 1106 60-61 -2.7667875491807763 1106 62-63 -3.506876474738842 1106 64-65 -3.1782901404136226 1106 66-67 -2.0671230605037074 1106 68-69 -3.032966075461335 1106 70-71 -4.006105346278989 1106 72-73 -3.9385266574371585 1106 74-75 -3.902757530232126 1106 76-77 -4.579959491933987 1106 78-79 -4.69467182399822 1106 80-81 -4.084821453033712 1106 82-83 -3.9751464452532304 1106 84-85 -3.4911092524153986 1106 86-87 -3.2986328139431436 1106 88-89 -3.79693618094144 1106 90-91 -3.2183715920720743 1106 92-93 -3.742841566958859 1106 94-95 -3.7471480351892126 1106 96-97 -3.285278857873376 1106 98-99 -2.8237850340785187 1106 100-101 -2.285313272121371 1107 1 -0.017924305980521638 1107 2 -0.3129677408332263 1107 3 0.1965967596822651 1107 4 -0.09256862795527354 1107 5 0.15960323206459037 1107 6 -0.16899820668699306 1107 7 0.3186402565988331 1107 8 0.38013381637020416 1107 9 -0.3040252041227305 1107 10-11 0.28129034479614745 1107 12-13 0.27337297215548517 1107 14-15 0.4670533170590332 1107 16-17 0.8539281744221157 1107 18-19 1.005565226719277 1107 20-21 0.7746497372792547 1107 22-23 0.8741008829179933 1107 24-25 0.9050999332790326 1107 26-27 0.789506809120887 1107 28-29 -0.020835813147918714 1107 30-31 0.4713775338518005 1107 32-33 0.9406062182458754 1107 34-35 -0.16044266652644268 1107 36-37 -0.7645760772239427 1107 38-39 -1.7125796928311061 1107 40-41 -0.9107503616063184 1107 42-43 -0.8976007708017804 1107 44-45 -0.6138235836865533 1107 46-47 -2.614744972876018 1107 48-49 -2.1347649930450743 1107 50-51 -1.9805143095070576 1107 52-53 -2.552372952864694 1107 54-55 -2.326340511582611 1107 56-57 -2.200901137990698 1107 58-59 -1.9705993565016584 1107 60-61 -2.2899535723468034 1107 62-63 -3.270969138831507 1107 64-65 -3.398367360490841 1107 66-67 -3.4991693925500336 1107 68-69 -2.6800703225655838 1107 70-71 -2.8088080489816925 1107 72-73 -2.517677236587737 1107 74-75 -2.082680310154906 1107 76-77 -0.7139363259108187 1107 78-79 -1.5054826348090309 1107 80-81 -1.2238175920298495 1107 82-83 -0.7504360205428071 1107 84-85 -0.9857038470099901 1107 86-87 -0.8032660185763483 1107 88-89 -0.8247354087406684 1107 90-91 0.07738130368081997 1107 92-93 -0.45365237776966794 1107 94-95 0.03007204203086289 1107 96-97 0.5641419915474764 1107 98-99 0.29320338290989767 1107 100-101 0.4224087356006372 1108 1 0.28291603015393463 1108 2 0.24501545244408618 1108 3 0.3898740706066377 1108 4 0.3191960779270815 1108 5 0.4285107950897995 1108 6 -0.228662072233206 1108 7 0.21359823979210546 1108 8 -0.04675693993231533 1108 9 0.41530252696971104 1108 10-11 -0.0275331846156206 1108 12-13 0.1973225519874191 1108 14-15 0.2804986952102908 1108 16-17 0.35308783828765655 1108 18-19 0.2610274115932256 1108 20-21 0.7927169641700118 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0.36896983358318636 2311 5 0.27315634561769997 2311 6 -0.29427293196171433 2311 7 -0.1868542488956777 2311 8 -0.0030163667799811833 2311 9 -0.1714244715219948 2311 10-11 -0.05204298853718825 2311 12-13 -0.44860504982253246 2311 14-15 -0.3695767195710076 2311 16-17 -0.10467988418594842 2311 18-19 0.11472273587678927 2311 20-21 0.4295947922243144 2311 22-23 0.1088994177165219 2311 24-25 0.28275561093470714 2311 26-27 0.14408556369964032 2311 28-29 -0.4966599889721053 2311 30-31 -0.7128715503972884 2311 32-33 -0.9447417671021014 2311 34-35 -2.00916061524439 2311 36-37 -3.64149915414702 2311 38-39 -3.3224698027212156 2311 40-41 -2.8404206912766483 2311 42-43 -2.8529121261131394 2311 44-45 -3.1361679060308774 2311 46-47 -2.3081515662826106 2311 48-49 -4.063336421616505 2311 50-51 -4.493701122693871 2311 52-53 -3.796695297187039 2311 54-55 -3.5003331855752826 2311 56-57 -4.009326046415605 2311 58-59 -4.781222067124368 2311 60-61 -5.963946246339475 2311 62-63 -5.974998442860812 2311 64-65 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-0.0193057104886023 2312 24-25 0.34685817503727634 2312 26-27 -0.3687349491208707 2312 28-29 0.2725707802586683 2312 30-31 0.36405152652578465 2312 32-33 0.786027463667125 2312 34-35 1.606224000140223 2312 36-37 0.16619315354528652 2312 38-39 1.0877866075352003 2312 40-41 1.9288100779541253 2312 42-43 1.6214468482458315 2312 44-45 2.1074218375588742 2312 46-47 3.114925356794309 2312 48-49 2.821278962998882 2312 50-51 2.929375800383049 2312 52-53 2.703304702812961 2312 54-55 2.5894104041683086 2312 56-57 2.74708420999465 2312 58-59 2.488008702106402 2312 60-61 2.946310163916934 2312 62-63 2.9224374545750855 2312 64-65 3.5704971083736297 2312 66-67 3.89753390415326 2312 68-69 4.381101838606579 2312 70-71 5.0347816946080535 2312 72-73 3.927377708467212 2312 74-75 3.9894808620062676 2312 76-77 5.463353051378558 2312 78-79 4.827850698524305 2312 80-81 4.6553032870910265 2312 82-83 4.720626250519462 2312 84-85 4.247629486323344 2312 86-87 3.9234739081635794 2312 88-89 3.907499023493763 2312 90-91 3.952106578406095 2312 92-93 4.318142494025203 2312 94-95 3.683551895510721 2312 96-97 2.131907559313042 2312 98-99 2.8019945917011064 2312 100-101 3.0667310799229845 2313 1 -0.39232906788528155 2313 2 -0.5760629789284657 2313 3 -0.1873318117463043 2313 4 -0.48840196128860924 2313 5 -1.0249205774592198 2313 6 -0.640426778115561 2313 7 -0.5474311719725975 2313 8 -0.4389138026774191 2313 9 -0.4695013945989217 2313 10-11 -0.46870965520385255 2313 12-13 -1.1825794087968902 2313 14-15 -1.6692562067504966 2313 16-17 -1.0966670636731237 2313 18-19 -0.6488990589950063 2313 20-21 0.045780689660212204 2313 22-23 0.0488032638703757 2313 24-25 -0.07942387624478187 2313 26-27 0.017482999597071114 2313 28-29 -0.22262152743363828 2313 30-31 0.13328229575655115 2313 32-33 -0.44153663889697725 2313 34-35 -0.8521093331931127 2313 36-37 -0.2833260772239399 2313 38-39 -0.3777582642596755 2313 40-41 -1.3604527425587065 2313 42-43 -1.0764698184208328 2313 44-45 -0.34209739321035926 2313 46-47 -0.34420925859030405 2313 48-49 -1.1883364216165049 2313 50-51 -1.1507524047451483 2313 52-53 -1.3928491433408823 2313 54-55 -3.3456857496778447 2313 56-57 -3.833043995133554 2313 58-59 -2.9575041184064226 2313 60-61 -3.767632143775373 2313 62-63 -2.833171519783889 2313 64-65 -1.649855455728936 2313 66-67 -2.1521455830262255 2313 68-69 -2.0107250844703444 2313 70-71 -2.3858913823150267 2313 72-73 -1.938808188968686 2313 74-75 -2.722858881583477 2313 76-77 -4.131198230672727 2313 78-79 -3.9325659681423666 2313 80-81 -3.7878056872679444 2313 82-83 -3.7304955443523298 2313 84-85 -3.8669538470099916 2313 86-87 -3.811301732862063 2313 88-89 -3.966699694454956 2313 90-91 -3.8836306010810837 2313 92-93 -3.9703190444363337 2313 94-95 -3.2026660532072277 2313 96-97 -2.629310389404907 2313 98-99 -2.308582331375817 2313 100-101 -2.0766984072565045 2314 1 -0.5480830361392499 2314 2 -0.5066185344840193 2314 3 -0.42245085936535176 2314 4 -0.7116162470028939 2314 5 -0.20547613301477696 2314 6 -0.6166172543060355 2314 7 -0.36389942594085056 2314 8 -0.835739199502811 2314 9 -0.11037441047193397 2314 10-11 -0.11553505202925152 2314 12-13 0.0019444007269129315 2314 14-15 -0.14247049246477417 2314 16-17 -0.06988134938740842 2314 18-19 0.018263639417689603 2314 20-21 -0.14122327859376327 2314 22-23 -0.13224832343122017 2314 24-25 0.0019253301044344084 2314 26-27 -0.03112811151403605 2314 28-29 0.22678323447112092 2314 30-31 -0.6524319899577264 2314 32-33 -0.09431441667475582 2314 34-35 -0.09218869827247289 2314 36-37 -0.3026713153191807 2314 38-39 -0.014166994418403078 2314 40-41 0.48131313045717405 2314 42-43 0.9706531974521866 2314 44-45 1.4718907020277356 2314 46-47 -0.11950687763792445 2314 48-49 -0.09508245336253651 2314 50-51 0.3671047381119905 2314 52-53 0.5158810153892723 2314 54-55 0.6815959963538951 2314 56-57 0.9625909255013667 2314 58-59 1.4532101673078657 2314 60-61 2.019570237177007 2314 62-63 1.5829991151367473 2314 64-65 1.752426290302811 2314 66-67 1.1960687026880592 2314 68-69 1.5929455504502918 2314 70-71 1.346747506573866 2314 72-73 1.4108941919836937 2314 74-75 2.0236688961943017 2314 76-77 2.522571610597115 2314 78-79 2.0659459366195385 2314 80-81 1.7126903444780872 2314 82-83 1.370198900092113 2314 84-85 0.8984231371169926 2314 86-87 0.5205435052331779 2314 88-89 0.3720899880847277 2314 90-91 0.5662701925697107 2314 92-93 0.5574587333414414 2314 94-95 0.449119661078484 2314 96-97 1.6117610391665202 2314 98-99 1.9503462400527525 2314 100-101 1.5160595292514323 2315 1 -0.5151524509698611 2315 2 -0.3142470585304551 2315 3 -0.19572733413436083 2315 4 -0.1671954936269131 2315 5 -0.47579122422538944 2315 6 -0.7075909572200416 2315 7 0.02439718623635656 2315 8 -0.2697595738216947 2315 9 -0.5820635836536425 2315 10-11 -0.644082789532213 2315 12-13 -0.7677784137720138 2315 14-15 -0.8691940176957687 2315 16-17 -0.311530247752728 2315 18-19 -0.7174624918308226 2315 20-21 -0.4068001561109398 2315 22-23 -0.7340014624977869 2315 24-25 -0.49298109017512814 2315 26-27 -0.7458876471690914 2315 28-29 -0.6545245125082673 2315 30-31 -1.2522898435469259 2315 32-33 -0.4434023105387652 2315 34-35 -0.6947710247353953 2315 36-37 -0.1690536891642367 2315 38-39 -0.47803811500594406 2315 40-41 -0.4041405535039786 2315 42-43 -0.28651335075913664 2315 44-45 -1.4790687862451861 2315 46-47 -0.5303100048589542 2315 48-49 -0.3991573171388936 2315 50-51 -0.9374439470337066 2315 52-53 -0.9438441682165042 2315 54-55 -1.024634754652972 2315 56-57 -1.0102828011037026 2315 58-59 -0.8424543671626381 2315 60-61 -0.8763075169097014 2315 62-63 -0.20615037550528115 2315 64-65 -0.37917759503241655 2315 66-67 -0.6219838914839393 2315 68-69 -0.4847611541220864 2315 70-71 -0.27597222808616806 2315 72-73 -0.1914512237945587 2315 74-75 0.20857768558069978 2315 76-77 0.24597838624270096 2315 78-79 -0.06518412734634538 2315 80-81 -0.6225382743326229 2315 82-83 -0.7732505194767114 2315 84-85 -0.30958444402491736 2315 86-87 0.3496124462424177 2315 88-89 0.34626672345548926 2315 90-91 -0.2597189095387975 2315 92-93 -0.5909658106054891 2315 94-95 -0.22536505818235497 2315 96-97 0.08866224741101192 2315 98-99 -1.0389927791370113 2315 100-101 -2.3532842281520274 2316 1 -0.3053192639637139 2316 2 0.021486040679377538 2316 3 0.04869760001840007 2316 4 0.1780196073388467 2316 5 -0.4067833225572599 2316 6 -0.4110150134096813 2316 7 -0.5452252896196583 2316 8 -0.6467569399323168 2316 9 -0.6905798259714686 2316 10-11 -1.1951802434391468 2316 12-13 -0.5703245068361085 2316 14-15 -0.7665601283191208 2316 16-17 0.13544077946412614 2316 18-19 -0.4566196472303048 2316 20-21 -0.7514006828888071 2316 22-23 -0.48808399103159417 2316 24-25 -0.10834544487222786 2316 26-27 0.040767313322568555 2316 28-29 -0.23512152743364112 2316 30-31 -1.2353451552238326 2316 32-33 -1.4165366388969716 2316 34-35 -1.1016191371146817 2316 36-37 -1.3528113713415877 2316 38-39 -0.8134200289655595 2316 40-41 -0.7073890170685075 2316 42-43 -0.5707100144992623 2316 44-45 -0.3978571971319269 2316 46-47 0.5348348590567511 2316 48-49 0.5719576960305588 2316 50-51 0.6320907325097522 2316 52-53 0.27871948411010194 2316 54-55 0.6610544464005876 2316 56-57 0.371367769572327 2316 58-59 -0.6285825497789723 2316 60-61 -0.14961743789302062 2316 62-63 -0.2029019119407529 2316 64-65 0.6083553285847927 2316 66-67 -0.21513577910465997 2316 68-69 0.05606413121592979 2316 70-71 0.9794272451359554 2316 72-73 0.9730790659332698 2316 74-75 -0.1129324109952421 2316 76-77 0.5087527497194309 2316 78-79 0.5180467769556749 2316 80-81 0.79466980292813 2316 82-83 0.504185828196686 2316 84-85 0.5201785059311845 2316 86-87 0.2765659141967589 2316 88-89 1.1719032467215165 2316 90-91 1.3235997910757789 2316 92-93 1.399288798700919 2316 94-95 1.2485594369888489 2316 96-97 1.261620983144116 2316 98-99 0.37303531568300485 2316 100-101 -0.26750723078591676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 3.0 6 4.0 7 15.0 8 30.0 9 70.0 10 131.0 11 250.0 12 337.0 13 490.0 14 651.0 15 926.0 16 1256.0 17 1836.0 18 1680.0 19 934.0 20 805.0 21 792.0 22 807.0 23 902.0 24 848.0 25 889.0 26 821.0 27 920.0 28 1019.0 29 1202.0 30 1443.0 31 1674.0 32 2014.0 33 2722.0 34 3987.0 35 5972.0 36 8625.0 37 13121.0 38 10181.0 39 793.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.87823993894385 21.64264537528987 17.18084950245105 17.29826518331523 2 15.581023375937312 25.135369128501623 39.25631355745667 20.027293938104393 3 18.403251603057917 30.535135214450264 32.5248345585538 18.53677862393802 4 11.525538127448536 21.4018458468446 38.58377474212434 28.488841283582527 5 11.037417461482026 40.39031548055759 33.96184886280264 14.61041819515774 6 25.38112775666515 41.197012603993954 17.69694657608616 15.724913063254736 7 23.249159978284155 38.87283024958549 22.025442753803944 15.852567018326413 8 25.315471299448294 34.01514262237352 20.11386312947529 20.5555229487029 9 26.604844981145366 18.759262248176896 21.831760890936568 32.80413187974117 10-11 22.961571757662906 29.628923158188194 26.846213666309627 20.563291417839274 12-13 26.48526110369316 28.709667952988127 27.56224964418294 17.24282129913577 14-15 22.74588059219697 29.029536484087277 29.615717576629052 18.608865347086702 16-17 17.83267060877731 32.69922087068801 29.44111044268044 20.026998077854238 18-19 17.535840584601388 29.963755887834008 35.44586127456015 17.054542253004445 20-21 19.654006148067175 27.98911249202145 35.3506525901851 17.006228769726274 22-23 18.923598374246183 28.95030299473244 35.06522090003375 17.06087773098763 24-25 19.04503367620948 29.04004460813805 35.62121234354136 16.29370937211111 26-27 18.35563772964724 29.307536538122907 36.13532312026766 16.201502611962198 28-29 17.16509897721103 30.090099344065035 36.36623769204807 16.37856398667586 30-31 18.047717248515717 30.00932034844893 35.21256999434908 16.73039240868627 32-33 17.689999486438303 30.813701826078667 34.77399617029706 16.722302517185973 34-35 17.853657820788424 30.07036104976632 35.205470406538666 16.87051072290659 36-37 18.46250926353944 30.102651023208377 34.777344867815714 16.657494845436467 38-39 17.612014761663694 30.91365307155592 35.12718361836844 16.347148548411948 40-41 16.963453735701762 31.506386975119778 34.54469415157052 16.985465137607946 42-43 17.286921812632073 31.787831650622213 33.39912538154496 17.52612115520075 44-45 17.798108597882628 30.8757822759921 33.20225090058033 18.123858225544932 46-47 18.31061350863549 31.40544982318156 32.31155262733129 17.972384040851665 48-49 18.69479090242113 31.644167278063094 31.557593543653706 18.103448275862068 50-51 17.95063684921054 31.929770499501085 31.8219170041674 18.297675647120972 52-53 18.046486375442047 32.434078269673805 30.985781155996417 18.53365419888773 54-55 17.58228052413024 32.041349356575836 30.894631039896698 19.48173907939722 56-57 18.672095667803823 31.629800814350173 30.512453688419356 19.185649829426655 58-59 18.68837304847987 30.994688343702315 30.57503815001761 19.74190045780021 60-61 17.837203330765565 31.586515535013387 31.446388613770587 19.12989252045046 62-63 17.823866887737882 30.603201619884672 32.08002582424838 19.492905668129062 64-65 17.56475163254824 30.926700418225845 31.975199941301636 19.53334800792428 66-67 17.692200465140164 30.24951761883451 31.24582731121105 20.812454604814274 68-69 17.537269632585982 30.201608169973 31.249266345815236 21.01185585162578 70-71 17.85965839056172 30.355256207078714 31.121236133122025 20.66384926923754 72-73 17.34769497171452 29.58272494478645 31.468423717247905 21.60115636625113 74-75 16.810347990051138 30.361636719810413 30.527451081094963 22.300564209043486 76-77 17.02678708995796 29.89720976984086 30.27946323103223 22.79653990916895 78-79 17.095415590514204 29.643108710965016 30.003375205447284 23.258100493073492 80-81 17.181195251859783 29.416900209822018 29.140316640500053 24.261587897818142 82-83 16.808023595357234 28.757575312917282 29.511805015480785 24.922596076244698 84-85 16.083782693224755 28.23447415722094 29.793477862147387 25.88826528740692 86-87 16.453420979267236 28.741214620046073 28.90995260663507 25.895411794051622 88-89 16.02729173544624 28.301969847034226 28.53600381497377 27.134734602545763 90-91 15.579765966032062 28.43035838743993 29.049557976596603 26.940317669931403 92-93 15.799976523066087 29.214843291466135 27.969832139922524 27.015348045545252 94-95 15.519606764241958 29.878581123216318 27.001210520523827 27.600601592017902 96-97 15.494549029389756 30.5122298358106 26.23802327116928 27.75519786363036 98-99 16.02435714023697 30.323905946223544 25.059975789589522 28.591761123949965 100-101 14.924102917764099 28.56723623104407 25.40222885776542 31.10643199342641 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 501.0 1 351.0 2 202.5 3 195.0 4 176.5 5 181.5 6 237.0 7 396.5 8 530.5 9 539.0 10 503.5 11 480.5 12 485.5 13 467.5 14 419.0 15 382.5 16 384.0 17 365.0 18 337.0 19 363.5 20 377.5 21 379.5 22 427.0 23 478.0 24 525.0 25 562.0 26 605.5 27 673.5 28 736.0 29 857.0 30 1001.0 31 1150.5 32 1340.5 33 1492.5 34 1613.0 35 1813.5 36 2084.0 37 2298.0 38 2391.5 39 2344.5 40 2281.0 41 2186.5 42 2064.0 43 2044.0 44 2019.5 45 2013.0 46 2071.0 47 2389.0 48 3008.5 49 2811.5 50 2094.5 51 1720.0 52 1417.0 53 1273.5 54 1206.5 55 1134.5 56 1038.0 57 920.5 58 826.5 59 725.5 60 601.5 61 492.5 62 398.0 63 288.0 64 197.0 65 156.0 66 124.5 67 90.5 68 64.0 69 34.5 70 19.5 71 14.5 72 11.5 73 6.5 74 3.0 75 3.0 76 2.0 77 0.0 78 0.0 79 0.0 80 1.0 81 1.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027878449958182325 2 0.008803721039425998 3 0.0029345736798086657 4 0.0 5 0.004401860519712999 6 0.0 7 0.0 8 0.0014672868399043329 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0058691473596173315 20-21 0.0022009302598564994 22-23 0.0 24-25 0.0058691473596173315 26-27 0.007336434199521665 28-29 0.008803721039425998 30-31 0.03301395389784749 32-33 0.0022009302598564994 34-35 0.006602790779569497 36-37 0.013939224979091162 38-39 0.005135503939665165 40-41 0.009537364459378164 42-43 0.013205581559138995 44-45 0.0036682170997608325 46-47 0.0058691473596173315 48-49 0.004401860519712999 50-51 0.007336434199521665 52-53 0.0058691473596173315 54-55 0.0029345736798086657 56-57 7.336434199521664E-4 58-59 0.0014672868399043329 60-61 7.336434199521664E-4 62-63 0.0 64-65 0.011738294719234663 66-67 0.0022009302598564994 68-69 0.0014672868399043329 70-71 0.007336434199521665 72-73 0.01247193813918683 74-75 0.006602790779569497 76-77 0.006602790779569497 78-79 0.013205581559138995 80-81 0.0 82-83 0.0058691473596173315 84-85 7.336434199521664E-4 86-87 0.0 88-89 7.336434199521664E-4 90-91 7.336434199521664E-4 92-93 0.0014672868399043329 94-95 7.336434199521664E-4 96-97 0.0 98-99 7.336434199521664E-4 100-101 0.0022009302598564994 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 68153.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.27234311035465 #Duplication Level Percentage of deduplicated Percentage of total 1 86.1161356152312 54.487696799847406 2 7.791846389314038 9.860167564157116 3 2.3653819396131905 4.489897730107259 4 1.0435508557117017 2.6411163118277994 5 0.6632345438523259 2.098220181063196 6 0.4429293632020779 1.6815107185303653 7 0.31074625481192897 1.3763150558302641 8 0.1994341635360141 1.009493345854181 9 0.16696813691387227 0.9508018722580077 >10 0.8070126617503827 8.853608791982746 >50 0.053337043736375864 2.259621733452673 >100 0.02550902091739715 2.3990139832435844 >500 0.009276007606326239 4.2830102856807475 >1k 0.004638003803163119 3.6095256261646593 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 1.8047628130823297 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1230 1.8047628130823297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 933 1.3689786216307427 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 749 1.0989978430883454 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 637 0.9346617170190601 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 600 0.8803721039425998 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 222 0.3257376784587619 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.30079380218038826 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 203 0.2978592285005796 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 150 0.22009302598564995 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 139 0.20395287074670226 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 132 0.19368186286737196 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 128 0.1878127155077546 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 128 0.1878127155077546 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 116 0.17020527342890263 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 107 0.15699969186976362 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 105 0.15406511818995494 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.14085953663081596 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.1393922497909116 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 94 0.13792496295100728 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 88 0.12912124191158128 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 87 0.12765395507167696 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 82 0.1203175208721553 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 71 0.10417736563320763 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 69 0.10124279195339897 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0014672868399043329 0.0 18-19 0.0 0.0 0.0 0.0014672868399043329 0.0 20-21 0.0 0.0 0.0 0.0029345736798086657 0.0 22-23 0.0 0.0 0.0 0.004401860519712999 0.0 24-25 0.0 0.0 0.0 0.008070077619473832 0.0 26-27 0.0 0.0 0.0 0.016140155238947664 0.0 28-29 0.0 0.0 0.0 0.03668217099760832 0.0 30-31 0.0 0.0 0.0 0.07556527225507315 0.0 32-33 0.0 0.0 0.0 0.09977550511349464 0.0 34-35 0.0 0.0 0.0 0.12398573797191612 0.0 36-37 0.0 0.0 0.0 0.1555324050298593 0.0 38-39 0.0 0.0 0.0 0.19441550628732412 0.0 40-41 0.0 0.0 0.0 0.30666294954000556 0.0 42-43 0.0 0.0 0.0 0.5142840373864687 0.0 44-45 0.0 0.0 0.0 0.7116341173536014 0.0 46-47 0.0 0.0 0.0 1.1907032705823664 0.0 48-49 0.0 0.0 0.0 1.5971417252358662 0.0 50-51 0.0 0.0 0.0 1.8583187827388377 0.0 52-53 0.0 0.0 0.0 2.181855530937743 0.0 54-55 0.0 0.0 0.0 2.4782474725984183 0.0 56-57 0.0 0.0 0.0 3.2463721332883364 0.0 58-59 0.0 0.0 0.0 4.142150749049932 0.0 60-61 0.0 0.0 0.0 5.098088125247605 0.0 62-63 0.0 0.0 0.0 6.175810309157336 0.0 64-65 0.0 0.0 0.0 7.3173594706029075 0.0 66-67 0.0 0.0 0.0 8.56455328452159 0.0 68-69 0.0 0.0 0.0 9.788270509001805 0.0 70-71 0.0 0.0 0.0 10.841782460053116 0.0 72-73 0.0 0.0 0.0 11.846873945387584 0.0 74-75 0.0 0.0 0.0 12.970815664754301 0.0 76-77 0.0 0.0 0.0 14.471850101976436 0.0 78-79 0.0 0.0 0.0 15.575249805584495 0.0 80-81 0.0 0.0 0.0 16.94863028773495 0.0 82-83 0.0 0.0 0.0 18.71891186007953 0.0 84-85 0.0 0.0 0.0 21.145804293281294 0.0 86-87 0.0 0.0 0.0 23.117104162692765 0.0 88-89 0.0 0.0 0.0 24.46040526462518 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCATATG 20 0.002067778 71.23185 9 TAAATGG 55 0.00256591 34.53665 7 TAGGGAG 35 0.008346552 27.155863 72-73 GAGTACT 825 0.0 25.902487 12-13 GTACTTT 840 0.0 25.157276 14-15 AGTACTT 835 0.0 23.31741 12-13 ACTTTTT 925 0.0 23.10222 16-17 GTATCAA 1710 0.0 23.066605 1 GGTATCA 1305 0.0 21.485384 1 TACTTTT 925 0.0 21.30538 14-15 AGAGTAC 1445 0.0 19.718157 10-11 ATGGGAT 170 7.830042E-5 19.553837 5 ATCAACG 2000 0.0 19.470037 3 CAACGCA 2005 0.0 19.421484 5 TCAACGC 2005 0.0 19.421484 4 TATCAAC 2005 0.0 19.184637 2 AACGCAG 2035 0.0 19.135172 6 GATTTAT 75 7.64872E-4 19.009104 36-37 ACATGGG 590 0.0 18.51223 3 GTACATG 595 0.0 18.370142 1 >>END_MODULE