##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936929_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68513 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3228730313955 33.0 31.0 34.0 31.0 34.0 2 32.422532949951105 34.0 31.0 34.0 31.0 34.0 3 32.478916410024375 34.0 31.0 34.0 31.0 34.0 4 36.14162275772481 37.0 35.0 37.0 35.0 37.0 5 36.09004130602951 37.0 35.0 37.0 35.0 37.0 6 36.18025776130077 37.0 36.0 37.0 35.0 37.0 7 36.13781326171676 37.0 35.0 37.0 35.0 37.0 8 36.139900456847606 37.0 36.0 37.0 35.0 37.0 9 37.8086786449287 39.0 38.0 39.0 35.0 39.0 10-11 37.75279144104039 39.0 37.0 39.0 35.0 39.0 12-13 37.66131245165151 39.0 37.0 39.0 35.0 39.0 14-15 38.728701122414726 40.0 38.0 41.0 34.5 41.0 16-17 38.6906207581043 40.0 38.0 41.0 34.5 41.0 18-19 38.83983331630493 40.0 38.0 41.0 35.0 41.0 20-21 38.910805248638944 40.0 38.0 41.0 35.0 41.0 22-23 38.79652766628231 40.0 38.0 41.0 35.0 41.0 24-25 38.633726446075926 40.0 38.0 41.0 35.0 41.0 26-27 38.37314086377768 40.0 37.5 41.0 34.0 41.0 28-29 38.198736006305374 40.0 37.0 41.0 34.0 41.0 30-31 37.99262913607636 40.0 37.0 41.0 34.0 41.0 32-33 37.63013588662005 40.0 35.0 41.0 33.5 41.0 34-35 37.33150642943674 39.5 35.0 41.0 33.0 41.0 36-37 37.061083298060225 39.0 35.0 41.0 33.0 41.0 38-39 36.7283581218163 39.0 35.0 41.0 33.0 41.0 40-41 36.415147490257326 39.0 35.0 41.0 31.5 41.0 42-43 35.960255717892956 39.0 35.0 41.0 30.5 41.0 44-45 35.34461343102768 39.0 35.0 41.0 28.5 41.0 46-47 34.518054967670366 38.0 35.0 40.0 22.0 41.0 48-49 33.758388918891306 38.0 34.0 40.0 11.0 41.0 50-51 33.26726314713996 38.0 33.5 40.0 2.0 41.0 52-53 32.93977055449331 38.0 33.0 40.0 2.0 41.0 54-55 32.47938347466904 38.0 33.0 40.0 2.0 41.0 56-57 32.09547093252375 37.0 33.0 40.0 2.0 41.0 58-59 31.681819508706376 36.5 32.0 40.0 2.0 41.0 60-61 31.22525652066031 36.0 31.0 39.5 2.0 41.0 62-63 30.821712667668912 36.0 31.0 39.0 2.0 40.5 64-65 30.342416767620744 35.0 30.5 39.0 2.0 40.0 66-67 29.83746150365624 35.0 29.0 39.0 2.0 40.0 68-69 29.462955935369926 35.0 29.0 38.0 2.0 40.0 70-71 28.914286339818723 35.0 28.5 37.5 2.0 40.0 72-73 28.347153095033057 34.5 26.5 37.0 2.0 39.0 74-75 27.852392976515407 34.0 26.0 36.0 2.0 39.0 76-77 26.540393793878536 32.5 25.0 35.0 2.0 37.0 78-79 27.08124005663159 34.0 25.5 35.0 2.0 37.5 80-81 26.652372542437202 34.0 23.0 35.0 2.0 37.0 82-83 26.35531213054457 34.0 22.5 35.0 2.0 37.0 84-85 26.02742545210398 33.5 20.0 35.0 2.0 36.0 86-87 25.76003094303271 33.0 20.0 35.0 2.0 36.0 88-89 25.388495613971074 33.0 18.0 35.0 2.0 35.0 90-91 24.942375899464338 33.0 9.5 35.0 2.0 35.0 92-93 24.54481631223271 32.5 2.0 35.0 2.0 35.0 94-95 24.006940288704335 32.0 2.0 34.0 2.0 35.0 96-97 23.363821464539576 31.5 2.0 34.0 2.0 35.0 98-99 22.73737830776641 31.0 2.0 34.0 2.0 35.0 100-101 19.999817552873175 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1248037264660482 1101 2 0.2337484470713349 1101 3 0.1322140369341156 1101 4 0.16951888934716663 1101 5 0.07997896792497272 1101 6 0.08462558015626342 1101 7 0.32935977440564557 1101 8 0.27240983156736576 1101 9 0.18131280289769336 1101 10-11 0.19996794624418612 1101 12-13 0.285636670813318 1101 14-15 0.40610569248376294 1101 16-17 0.4831942304910015 1101 18-19 0.5288572732303791 1101 20-21 0.40776886390898426 1101 22-23 0.6018669534481518 1101 24-25 0.6666498448822509 1101 26-27 0.7350522619965503 1101 28-29 1.1107451561254393 1101 30-31 0.8962215664523399 1101 32-33 0.9311486759603369 1101 34-35 0.88699984336548 1101 36-37 1.0060292311480055 1101 38-39 1.3966918029158961 1101 40-41 0.916561536311491 1101 42-43 0.7582089294006451 1101 44-45 1.201798252388329 1101 46-47 1.8034084904513463 1101 48-49 2.102669794807042 1101 50-51 2.100704698586547 1101 52-53 2.2257614350423154 1101 54-55 2.0085104278250157 1101 56-57 2.499164234576366 1101 58-59 2.6793439382057436 1101 60-61 2.762280854861629 1101 62-63 2.2836474365066124 1101 64-65 2.494073400877731 1101 66-67 2.635981257868284 1101 68-69 2.6586921272990764 1101 70-71 2.7345960909502516 1101 72-73 2.8374782110573626 1101 74-75 2.349414280337033 1101 76-77 2.2890650276450053 1101 78-79 2.2771674661111057 1101 80-81 2.802701740819849 1101 82-83 2.6519435958510478 1101 84-85 2.536412507864931 1101 86-87 2.9166225613980394 1101 88-89 2.6976916377579734 1101 90-91 2.9295323755992904 1101 92-93 3.0715178071417917 1101 94-95 3.0373199585715795 1101 96-97 2.868403730361198 1101 98-99 3.05682712971711 1101 100-101 2.7978025689209467 1102 1 0.09144445784126987 1102 2 0.32789355502698214 1102 3 0.38683376072326325 1102 4 0.30198339041699995 1102 5 0.16887217936243104 1102 6 -0.02478893063930343 1102 7 0.17501237117669888 1102 8 0.1069750912444718 1102 9 0.3442188523044223 1102 10-11 0.18970729656513896 1102 12-13 0.19698660273318325 1102 14-15 0.3187685985331683 1102 16-17 0.4100567086080886 1102 18-19 0.14950500713428738 1102 20-21 0.18164554159815083 1102 22-23 -0.14745224515134225 1102 24-25 0.0975485027309233 1102 26-27 0.03941911669407716 1102 28-29 -0.41410409499301437 1102 30-31 -0.23342247167254015 1102 32-33 -0.12662218573972694 1102 34-35 -0.22562415974675076 1102 36-37 -0.376727041950609 1102 38-39 -0.7493887261640495 1102 40-41 -0.06554311258950207 1102 42-43 0.07998484848254606 1102 44-45 -0.022914838450034836 1102 46-47 -0.2142437172903371 1102 48-49 0.22360930073973861 1102 50-51 0.6391699777151274 1102 52-53 0.4261115614768656 1102 54-55 -0.037006008665940726 1102 56-57 0.2362290079667595 1102 58-59 0.9376108123547375 1102 60-61 0.8788437803625015 1102 62-63 0.7483430210232456 1102 64-65 0.8697298879425013 1102 66-67 0.18135180834484288 1102 68-69 0.8174161109598401 1102 70-71 0.7770489211389311 1102 72-73 0.4909502981999374 1102 74-75 0.9076714287517405 1102 76-77 1.1166763873026575 1102 78-79 1.0069379387817925 1102 80-81 0.9905542986879681 1102 82-83 0.8786504622194578 1102 84-85 1.58932050241852 1102 86-87 0.9846540727771504 1102 88-89 1.4009594844804028 1102 90-91 1.1442279601159235 1102 92-93 1.0238130804349268 1102 94-95 2.014221339625852 1102 96-97 1.8521131254205265 1102 98-99 1.1639074642823672 1102 100-101 0.5634610011325805 1103 1 0.1049215467900595 1103 2 0.39415590902518716 1103 3 0.17075110124437742 1103 4 0.20584682258231624 1103 5 0.1428164740614477 1103 6 0.06977531015135696 1103 7 0.20196654907427103 1103 8 0.39538479474851584 1103 9 -0.13017467869288168 1103 10-11 0.22396156430996683 1103 12-13 0.44193269437738536 1103 14-15 0.34572277643074756 1103 16-17 0.3975904013304685 1103 18-19 0.5152956630192804 1103 20-21 0.6433481471686804 1103 22-23 0.3797265508953771 1103 24-25 0.04723403732212006 1103 26-27 -0.0035952588674703634 1103 28-29 0.14744127787311356 1103 30-31 0.7066044825053552 1103 32-33 0.8638315429215524 1103 34-35 1.2153012670277334 1103 36-37 0.5555505860817291 1103 38-39 1.017457635021934 1103 40-41 1.2959797984617083 1103 42-43 1.3833316589048223 1103 44-45 1.62859010314925 1103 46-47 1.756218996096905 1103 48-49 1.0746874678556466 1103 50-51 1.5438195734024518 1103 52-53 2.4584565749539564 1103 54-55 1.9188565250267828 1103 56-57 2.582927121174304 1103 58-59 2.504883948024098 1103 60-61 2.7541807075862224 1103 62-63 2.4749827335120145 1103 64-65 3.3053767882120475 1103 66-67 3.3132026618938113 1103 68-69 2.545965077716353 1103 70-71 2.936977043331204 1103 72-73 2.7343240628001197 1103 74-75 1.9026175203959461 1103 76-77 2.26267818424785 1103 78-79 2.2809720807404616 1103 80-81 1.790981073171345 1103 82-83 1.542284783872649 1103 84-85 1.5008209516548199 1103 86-87 1.555184171609131 1103 88-89 2.3457034197903788 1103 90-91 2.1030105297475465 1103 92-93 1.8132335656101297 1103 94-95 1.4993965417821862 1103 96-97 1.8114572404250175 1103 98-99 2.0144240860254072 1103 100-101 1.7421447387785811 1104 1 0.20177384703218593 1104 2 0.0779735038597309 1104 3 0.4738180907520473 1104 4 0.18445860627884514 1104 5 0.14200937155942484 1104 6 0.2535929049859007 1104 7 0.09906098006700859 1104 8 0.16293927416740672 1104 9 0.5792684205807248 1104 10-11 0.3366532511541962 1104 12-13 0.3950198614476008 1104 14-15 0.009766359965851734 1104 16-17 0.35249354903022123 1104 18-19 0.03174037649790051 1104 20-21 0.09482110923486431 1104 22-23 0.12165552587520523 1104 24-25 -0.13638178188691796 1104 26-27 -0.44619009341145244 1104 28-29 0.051597855758508615 1104 30-31 -0.29218486374161046 1104 32-33 0.2210752878771629 1104 34-35 0.43907043571215354 1104 36-37 -0.6874881548383698 1104 38-39 -0.3741888540821776 1104 40-41 -0.9640080950007572 1104 42-43 -0.4006675339926744 1104 44-45 -0.3228828589169339 1104 46-47 0.6986120550153885 1104 48-49 0.351927177298748 1104 50-51 0.3522336169859912 1104 52-53 0.10191904468761237 1104 54-55 -0.430114419283143 1104 56-57 0.004537290665830085 1104 58-59 0.33367732978358333 1104 60-61 -0.6459403577890797 1104 62-63 -0.036114925890728955 1104 64-65 -0.7058217590034523 1104 66-67 -0.4172251024322584 1104 68-69 -1.025564381524969 1104 70-71 -1.087437807354835 1104 72-73 -0.39168481532740174 1104 74-75 -0.43243090575417753 1104 76-77 -0.607580088552794 1104 78-79 -0.47427408552265504 1104 80-81 -0.4080705813887846 1104 82-83 -0.597444836789176 1104 84-85 -0.41483683688432293 1104 86-87 -0.7896503723779524 1104 88-89 -0.5768147400159194 1104 90-91 -0.6487045630692414 1104 92-93 -0.46491413414774385 1104 94-95 -0.21731048000958353 1104 96-97 -0.7264765771698158 1104 98-99 0.3363570965177374 1104 100-101 0.6300583788108653 1105 1 0.39728035077676793 1105 2 0.10871847823892011 1105 3 0.4359781222853556 1105 4 0.29167185026781084 1105 5 0.486116584803419 1105 6 0.028523925876712042 1105 7 -0.038897238301139225 1105 8 -0.22255540458702683 1105 9 0.4113535605097738 1105 10-11 0.3459161601017726 1105 12-13 -0.05432976409437629 1105 14-15 0.26183573324136944 1105 16-17 0.018437577410203687 1105 18-19 0.1629977671167424 1105 20-21 -0.22445362470286057 1105 22-23 0.08598347226857328 1105 24-25 -0.014781466553849043 1105 26-27 0.4367425041447106 1105 28-29 0.6992131494124223 1105 30-31 0.9590961768575141 1105 32-33 0.5821316536635166 1105 34-35 1.4132525405604 1105 36-37 1.5393545727926963 1105 38-39 1.4468596283774176 1105 40-41 1.1751326223819731 1105 42-43 1.2024069634452488 1105 44-45 1.8801128052533471 1105 46-47 1.8261248654213773 1105 48-49 2.0115645442676 1105 50-51 1.8108461514755447 1105 52-53 2.6519781696383404 1105 54-55 2.257311674528445 1105 56-57 2.905601539778953 1105 58-59 3.3535827298624135 1105 60-61 4.044047817220772 1105 62-63 3.817452279469933 1105 64-65 3.356456522431319 1105 66-67 2.9416633484940284 1105 68-69 2.4040769271072726 1105 70-71 2.1183170211828077 1105 72-73 2.1372864105299065 1105 74-75 2.917982968900926 1105 76-77 3.187650498755051 1105 78-79 3.797306521493507 1105 80-81 4.1812080942123195 1105 82-83 4.141814130495018 1105 84-85 3.4631686814444116 1105 86-87 2.92201695123261 1105 88-89 2.726102090886723 1105 90-91 3.2249651255393523 1105 92-93 2.6957086486666064 1105 94-95 1.7917553457688946 1105 96-97 2.283771747623245 1105 98-99 2.7462900882402472 1105 100-101 2.1127150599302986 1106 1 0.4192072610757691 1106 2 0.7060606709299506 1106 3 0.48979872029199356 1106 4 0.3820372987727936 1106 5 0.24757837882334854 1106 6 0.36739435777040086 1106 7 0.5305379776457002 1106 8 0.3668133661770909 1106 9 0.3841110355928308 1106 10-11 0.5180091833575844 1106 12-13 0.8383612658059505 1106 14-15 1.252865633573606 1106 16-17 0.9440189727590322 1106 18-19 0.8450575677811898 1106 20-21 0.6921576709782045 1106 22-23 0.30591702708585444 1106 24-25 0.623424513512596 1106 26-27 -0.0905000207722324 1106 28-29 0.030774611206446423 1106 30-31 0.16374733964821786 1106 32-33 0.17216487625488242 1106 34-35 0.7700631717896371 1106 36-37 0.6662648717960167 1106 38-39 0.11031477787907562 1106 40-41 -0.05044877296685968 1106 42-43 0.610712611285777 1106 44-45 1.3226377221968662 1106 46-47 1.3835999484778512 1106 48-49 1.3604017535699313 1106 50-51 1.5473910019738852 1106 52-53 -0.3486862821889005 1106 54-55 -0.17757204640178514 1106 56-57 -0.8920728788256973 1106 58-59 -0.15106843292828032 1106 60-61 0.24703785044336612 1106 62-63 1.1868874954167765 1106 64-65 -0.33390892607367206 1106 66-67 -0.84870210001095 1106 68-69 -0.648082541331263 1106 70-71 -0.07968962333546159 1106 72-73 -1.1311521276760708 1106 74-75 -0.9152396224611969 1106 76-77 -1.1492265776569077 1106 78-79 -0.9452183954500164 1106 80-81 0.06360012079039379 1106 82-83 -1.3993818827940174 1106 84-85 -1.9467980959642297 1106 86-87 -1.7281491617242004 1106 88-89 -1.2400108659239102 1106 90-91 -1.2457989940619747 1106 92-93 -1.433195005818444 1106 94-95 -1.0434606010749583 1106 96-97 -0.7361618071940264 1106 98-99 -0.19390924730792491 1106 100-101 0.3409542625881059 1107 1 0.0587030593950999 1107 2 0.032111091098016686 1107 3 -0.0715458175231305 1107 4 -0.5448534575297259 1107 5 -0.007043469916148126 1107 6 0.06067166869477347 1107 7 0.4624707507549459 1107 8 0.08866210567288846 1107 9 0.18495137172728704 1107 10-11 -0.1937555225247607 1107 12-13 0.20558815656226415 1107 14-15 -0.0849494924768166 1107 16-17 0.652842502170806 1107 18-19 -0.177631507849064 1107 20-21 -0.03977510213103841 1107 22-23 0.04289181700182354 1107 24-25 -0.2959032175798413 1107 26-27 -0.30436556699071815 1107 28-29 -1.288132951818767 1107 30-31 -1.6715467779988415 1107 32-33 -1.0727931069383985 1107 34-35 -1.6992645593027973 1107 36-37 -1.3614662206409562 1107 38-39 -0.20943312128058267 1107 40-41 -0.5033899494374552 1107 42-43 0.48592269531938115 1107 44-45 -0.15887488284515427 1107 46-47 -0.42900509353894734 1107 48-49 -1.2278335405477172 1107 50-51 -1.9005081576899805 1107 52-53 -1.4995266183233582 1107 54-55 -1.3216896934606126 1107 56-57 -0.4097199376492249 1107 58-59 -0.4863625505753397 1107 60-61 -1.9432982840104174 1107 62-63 -0.637062084415156 1107 64-65 -1.35113581682997 1107 66-67 -2.942399579002551 1107 68-69 -2.7905195161211793 1107 70-71 -3.2506980266968064 1107 72-73 -3.3059420436424567 1107 74-75 -2.9954917233015372 1107 76-77 -3.0517475860602694 1107 78-79 -3.369588143349173 1107 80-81 -2.796904080890279 1107 82-83 -2.045600370188975 1107 84-85 -2.412344314451623 1107 86-87 -3.2445357163460464 1107 88-89 -2.385389017184412 1107 90-91 -1.682353615910717 1107 92-93 -2.1668084511965944 1107 94-95 -1.7674101809068894 1107 96-97 -1.2172542441688172 1107 98-99 -1.5300437010894399 1107 100-101 -2.8380373340505507 1108 1 0.22473721499282107 1108 2 -0.12158909865530632 1108 3 0.2851904253611224 1108 4 0.380193980800442 1108 5 0.0982696230629827 1108 6 0.11854643150311972 1108 7 0.23192046612496853 1108 8 0.46911751364253007 1108 9 0.639410574763339 1108 10-11 0.5728478930350107 1108 12-13 0.09458246396263803 1108 14-15 0.39987024186853404 1108 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-1.1231503629613009 2316 94-95 -0.6010498867892444 2316 96-97 0.5741060499488277 2316 98-99 0.039349681263502134 2316 100-101 0.16104354830239132 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 10.0 7 18.0 8 21.0 9 72.0 10 145.0 11 243.0 12 338.0 13 426.0 14 582.0 15 918.0 16 1297.0 17 1947.0 18 1602.0 19 952.0 20 800.0 21 814.0 22 812.0 23 881.0 24 882.0 25 894.0 26 908.0 27 998.0 28 1062.0 29 1319.0 30 1470.0 31 1706.0 32 2161.0 33 2942.0 34 4184.0 35 6344.0 36 9121.0 37 13011.0 38 9046.0 39 586.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.854194038130274 21.632945023502963 17.090011970453418 17.422848967913346 2 15.445469406819242 25.3108944418496 39.25005838393274 19.993577767398413 3 18.73075182812021 30.620466188898458 32.22308175090859 18.425700232072746 4 11.643045845314028 21.01790900996891 39.3005706946127 28.03847445010436 5 11.089866156787762 40.69884547458147 33.510428677769184 14.70085969086159 6 25.44918482623736 41.1381781559704 17.59666048779064 15.815976530001604 7 23.34009604016756 38.96048925021528 22.154919504327648 15.544495205289508 8 25.598061681724637 33.90013574066236 20.07064352750573 20.43115905010728 9 26.736531753097953 18.88108826062207 21.544816312232715 32.83756367404726 10-11 23.03930513484909 29.64636327141345 26.67639867158133 20.63793292215613 12-13 26.919708668427887 28.727394800986673 27.10215579525054 17.250740735334897 14-15 22.71904602046327 29.054340052252858 29.402449170230465 18.824164757053406 16-17 18.076131537080553 32.60111219768511 29.460102462306427 19.862653802927912 18-19 17.62889983579639 30.03393541324576 35.122787812442986 17.21437693851487 20-21 19.546655379271087 27.97717221549195 35.29184242406551 17.184329981171455 22-23 18.984995329285383 28.79203643157403 35.12961233068659 17.093355908453994 24-25 19.30092183725157 29.025830419899425 35.49255888299309 16.18068885985592 26-27 18.450399953290127 29.199509546330354 36.019880889823085 16.33020961055643 28-29 17.220819406535988 30.22711018346883 35.959598908236394 16.59247150175879 30-31 17.908672266699753 30.005911416331564 35.19920013428403 16.886216182684656 32-33 17.713685193834657 30.96099953292854 34.478631480616535 16.846683792620272 34-35 18.066644286485776 29.994307649643133 34.935705632507705 17.003342431363393 36-37 18.718708490855214 30.194572975813394 34.292574698214835 16.794143835116554 38-39 17.73859224009576 30.55498788427291 35.063352115143196 16.64306776048813 40-41 17.08726810970181 31.50715922525652 34.27816618743888 17.12740647760279 42-43 17.506166712886607 31.521754995402336 33.228000525447726 17.744077766263334 44-45 18.076861325587846 30.71168975230978 32.93170639148775 18.27974253061463 46-47 18.68915388320465 31.376527082451506 31.96546640783501 17.968852626508838 48-49 19.091546006539 31.379904250350304 31.52951307800093 17.99903666510976 50-51 18.280213404176124 31.791675485523697 31.635490486581958 18.292620623718225 52-53 18.11335075095237 32.55440573321851 30.689796097091065 18.64244741873805 54-55 18.001751569113996 31.75448839585462 30.447379944533644 19.79638009049774 56-57 18.586931930142097 31.649893082082308 30.44350865925661 19.319666328518988 58-59 18.99521251751518 30.99967888836992 30.334102055114432 19.671006539000466 60-61 17.92803970223325 31.505619617574077 31.288133119252663 19.278207560940007 62-63 18.19024404483886 30.606317141522656 31.70320527790752 19.500233535730967 64-65 17.790914066776136 30.97974822112753 31.73362525086663 19.495712461229704 66-67 17.8041001612915 30.521314562214 30.90739240543282 20.767192871061678 68-69 17.855996029892573 30.213685193834657 30.942754553946756 20.987564222326014 70-71 18.08939974457216 30.018609742747675 30.75497172048896 21.137018792191206 72-73 17.505436924379314 29.29808941368791 31.45680381825347 21.7396698436793 74-75 16.987287084202997 30.429261599988322 30.269437916891683 22.314013398916995 76-77 17.319340855020215 29.56592179586356 30.25046341570213 22.864273933414097 78-79 17.369853815894146 29.66230960669688 30.0710120494238 22.89682452798517 80-81 17.288239501379383 29.49393510341707 29.197623669882788 24.02020172532076 82-83 17.020810674686306 28.399891968437263 29.483857310744032 25.095440046132396 84-85 16.251487128582795 28.09085534526929 29.970293922297074 25.687363603850844 86-87 16.495755815226516 28.861186328105042 28.86775514374758 25.775302712920862 88-89 16.057275474011472 28.264804192027558 28.731882471427944 26.946037862533025 90-91 15.92517989811855 28.231232940695655 29.03767278247289 26.80591437871291 92-93 15.995621237000549 29.03411786170407 28.200693304141577 26.769567597153802 94-95 15.667829463021615 29.60095164421351 27.22549005298265 27.50572883978223 96-97 15.691369017209416 30.52591630296749 26.251295450232814 27.53141922959027 98-99 16.19860164357968 30.748514793676744 24.681428717394795 28.371454845348786 100-101 15.178252143769386 28.77796022623609 25.051632913701877 30.992154716292646 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 491.0 1 343.5 2 193.5 3 187.5 4 170.5 5 181.5 6 248.5 7 364.5 8 501.0 9 548.0 10 486.0 11 445.5 12 446.5 13 427.0 14 405.5 15 388.5 16 383.0 17 379.0 18 378.5 19 373.5 20 357.0 21 376.0 22 421.0 23 483.5 24 520.5 25 540.0 26 605.0 27 676.5 28 736.0 29 839.5 30 1036.5 31 1195.0 32 1333.0 33 1448.0 34 1551.5 35 1814.0 36 2112.0 37 2286.0 38 2426.5 39 2423.0 40 2266.0 41 2150.0 42 2058.0 43 2069.0 44 2068.0 45 2058.5 46 2095.0 47 2426.5 48 3021.5 49 2810.5 50 2131.5 51 1694.0 52 1458.0 53 1352.5 54 1190.5 55 1131.0 56 1067.0 57 963.0 58 862.5 59 746.5 60 637.5 61 528.5 62 410.5 63 293.0 64 201.5 65 151.0 66 121.5 67 102.0 68 69.5 69 31.5 70 17.0 71 11.5 72 11.5 73 7.0 74 6.5 75 8.0 76 3.5 77 1.5 78 1.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01605534716039292 2 0.0014595770145811745 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01532555865310233 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.297885072905872E-4 20-21 0.0 22-23 0.0014595770145811745 24-25 0.012406404623939982 26-27 0.007297885072905873 28-29 0.0 30-31 0.0021893655218717615 32-33 0.0014595770145811745 34-35 0.0 36-37 0.005838308058324698 38-39 0.010217039102068221 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0014595770145811745 50-51 0.0065680965656152845 52-53 0.0 54-55 0.004378731043743523 56-57 0.0036489425364529364 58-59 0.0014595770145811745 60-61 0.004378731043743523 62-63 0.0014595770145811745 64-65 7.297885072905872E-4 66-67 0.0051085195510341104 68-69 0.0014595770145811745 70-71 7.297885072905872E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0051085195510341104 80-81 0.005838308058324698 82-83 0.02116386671142703 84-85 0.012406404623939982 86-87 0.010946827609358807 88-89 0.002919154029162349 90-91 0.002919154029162349 92-93 7.297885072905872E-4 94-95 0.0 96-97 0.005838308058324698 98-99 0.005838308058324698 100-101 7.297885072905872E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 68513.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.05956533796506 #Duplication Level Percentage of deduplicated Percentage of total 1 86.09388019627812 54.29042663436137 2 7.811776687343765 9.852144848422926 3 2.2960836959540782 4.3437011953935745 4 1.0878622349782427 2.744004787412608 5 0.6457735394870845 2.0361099353407384 6 0.4374594944912508 1.6551603345350516 7 0.31015646699379684 1.3690832396771415 8 0.2036848439959263 1.0275422182651468 9 0.13887602999722248 0.7881715878738341 >10 0.895750393482085 9.827332039175047 >50 0.041662808999166745 2.0200545881803453 >100 0.02314600499953708 2.2915359128924435 >500 0.009258401999814833 4.158334914541766 >1k 0.004629200999907416 3.596397763928014 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1250 1.8244712682264679 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1214 1.771926495701546 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 987 1.4406025133916192 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 672 0.9808357537985491 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 621 0.9063973260549092 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 569 0.8304993212966882 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 232 0.33862186738283245 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 207 0.3021324420183031 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 193 0.2816983638141667 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 171 0.24958766949338082 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 157 0.2291535912892444 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 136 0.19850247398303972 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 131 0.19120458891013384 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 0.1824471268226468 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 114 0.16639177966225388 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 104 0.15179600951644212 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 100 0.14595770145811746 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 99 0.14449812444353627 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 97 0.14157897041437392 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 94 0.1372002393706304 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.13428108534146804 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 91 0.13282150832688686 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 91 0.13282150832688686 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 80 0.11676616116649396 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 73 0.10654912206442572 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 72 0.10508954504984455 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 72 0.10508954504984455 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 69 0.10071081400610103 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.002919154029162349 0.0 24-25 0.0 0.0 0.0 0.008757462087487046 0.0 26-27 0.0 0.0 0.0 0.018974501189555265 0.0 28-29 0.0 0.0 0.0 0.03211069432078584 0.0 30-31 0.0 0.0 0.0 0.06714054267073402 0.0 32-33 0.0 0.0 0.0 0.09414271744048575 0.0 34-35 0.0 0.0 0.0 0.12625341176127158 0.0 36-37 0.0 0.0 0.0 0.1605534716039292 0.0 38-39 0.0 0.0 0.0 0.21747697517259498 0.0 40-41 0.0 0.0 0.0 0.32621546275889246 0.0 42-43 0.0 0.0 0.0 0.5429626494241968 0.0 44-45 0.0 0.0 0.0 0.707165063564579 0.0 46-47 0.0 0.0 0.0 1.1443083794316407 0.0 48-49 0.0 0.0 0.0 1.5449622699341732 0.0 50-51 0.0 0.0 0.0 1.7865222658473574 0.0 52-53 0.0 0.0 0.0 2.069680206676105 0.0 54-55 0.0 0.0 0.0 2.342621108402785 0.0 56-57 0.0 0.0 0.0 3.1001415789704145 0.0 58-59 0.0 0.0 0.0 3.961292017573307 0.0 60-61 0.0 0.0 0.0 4.804927532001226 0.0 62-63 0.0 0.0 0.0 5.750733437449828 0.0 64-65 0.0 0.0 0.0 6.797250156904529 0.0 66-67 0.0 0.0 0.0 8.058324697502664 0.0 68-69 0.0 0.0 0.0 9.303343890940406 0.0 70-71 0.0 0.0 0.0 10.387079824266927 0.0 72-73 0.0 0.0 0.0 11.394917752835228 0.0 74-75 0.0 0.0 0.0 12.507845226453373 0.0 76-77 0.0 0.0 0.0 13.984207376702232 0.0 78-79 0.0 0.0 0.0 15.095675273305796 0.0 80-81 0.0 0.0 0.0 16.40637543239969 0.0 82-83 0.0 0.0 0.0 18.09510603827011 0.0 84-85 0.0 0.0 0.0 20.573467809028944 0.0 86-87 0.0 0.0 0.0 22.653365054807118 0.0 88-89 0.0 0.0 0.0 24.120239954461198 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATTCA 15 6.5748446E-4 95.059166 9 TAGCCAC 25 5.223816E-5 76.04733 5 GCTCTAT 20 0.0020666162 71.24233 1 CTCTATT 20 0.0020666162 71.24233 2 GTGGTAC 20 0.0020666162 71.24233 3 TGGTACT 20 0.0020666162 71.24233 4 ATTCTAT 40 5.336123E-4 47.529583 6 TGGGATA 35 2.0364692E-4 33.949703 20-21 ATAACCC 60 0.0039273594 31.663261 3 AATAAGG 65 0.005809339 29.227625 2 AATTGGT 35 0.008340855 27.159761 22-23 CAATAAG 70 0.008341933 27.139938 1 ATGGGAT 185 8.600182E-9 25.691668 5 TGGGATG 135 1.3125455E-5 24.64497 6 GTACATG 575 0.0 23.953945 1 TACATGG 615 0.0 22.395966 2 GAGTACT 785 0.0 22.386127 12-13 ATGTAGA 55 0.0028663764 21.588589 60-61 ACATGGG 550 0.0 21.588587 3 GTACTTT 855 0.0 20.831093 14-15 >>END_MODULE