FastQCFastQC Report
Thu 26 May 2016
SRR936928_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936928_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72530
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16722.305252998759134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12551.7303184889011443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8641.1912312146697916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7461.0285399145181304No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6930.9554667034330621No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2990.41224320970632844No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT2830.39018337239762857No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2620.36122983592996No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.3308975596304977No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.3033227629946229No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.24265821039569832No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1610.2219771129187922No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT1420.19578105611471114No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.19302357645112367No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC1400.19302357645112367No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1370.18888735695574246No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1300.17923617813318626No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.14476768233834275No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC880.12132910519784916No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG820.11305666620708672No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAA810.11167792637529299No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.10754170687991176No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT760.10478422721632429No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC760.10478422721632429No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA760.10478422721632429No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA750.10340548738453054No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT730.10064800772094307No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA730.10064800772094307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCAT200.00206091871.29616
TAACTCA250.00498955257.0368844
ATGGGAG704.2355186E-640.7406275
GTATCAA12150.032.8607521
GGTATCA8050.029.5221941
GTGCAGT650.00579011829.249681
TATCAAC14700.027.160422
ATCAACG14700.027.160423
CAACGCA14750.027.0683485
TCAACGC14750.027.0683484
AACGCAG14850.026.8860726
GAGTACT7850.026.62304312-13
CGCAGAG16150.024.4107028
ACGCAGA16200.024.3353587
AGTACTT8100.024.33535812-13
GTACTTT8650.024.16079514-15
GCAGAGT15800.024.0495839
TGGGATG1050.0021683822.6336826
AGAGTAC12700.021.87896710-11
ACTTTTT9750.021.67854316-17
Produced by FastQC (version 0.11.5)