##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936928_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 72530 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23018061491796 33.0 31.0 34.0 31.0 34.0 2 32.3299738039432 33.0 31.0 34.0 31.0 34.0 3 32.38979732524473 34.0 31.0 34.0 31.0 34.0 4 36.10009651178822 37.0 35.0 37.0 35.0 37.0 5 35.993492347993936 37.0 35.0 37.0 35.0 37.0 6 36.12600303322763 37.0 35.0 37.0 35.0 37.0 7 36.08067006755825 37.0 35.0 37.0 35.0 37.0 8 36.062360402592034 37.0 35.0 37.0 35.0 37.0 9 37.746628981111265 39.0 37.0 39.0 35.0 39.0 10-11 37.6827795395009 39.0 37.0 39.0 35.0 39.0 12-13 37.57157727836757 39.0 37.0 39.0 35.0 39.0 14-15 38.636391837860195 40.0 38.0 41.0 34.0 41.0 16-17 38.64391975734179 40.0 38.0 41.0 34.5 41.0 18-19 38.768406176754446 40.0 38.0 41.0 35.0 41.0 20-21 38.81369778022887 40.0 38.0 41.0 35.0 41.0 22-23 38.690893423411 40.0 38.0 41.0 35.0 41.0 24-25 38.551096098166276 40.0 38.0 41.0 34.5 41.0 26-27 38.25928581276713 40.0 37.0 41.0 34.0 41.0 28-29 38.05635599062457 40.0 37.0 41.0 34.0 41.0 30-31 37.81882669240315 39.5 36.5 41.0 34.0 41.0 32-33 37.453536467668556 39.0 35.0 41.0 33.0 41.0 34-35 37.15654901420102 39.0 35.0 41.0 33.0 41.0 36-37 36.90821039569833 39.0 35.0 41.0 33.0 41.0 38-39 36.570653522680274 39.0 35.0 41.0 32.5 41.0 40-41 36.04766303598511 38.5 35.0 41.0 30.5 41.0 42-43 35.63843926651041 38.0 35.0 40.0 30.0 41.0 44-45 35.100303322763 38.0 35.0 40.0 27.0 41.0 46-47 34.25675582517579 38.0 34.5 40.0 21.5 41.0 48-49 33.4947745760375 38.0 34.0 40.0 7.5 41.0 50-51 32.982372811250514 38.0 33.0 40.0 2.0 41.0 52-53 32.62415552185303 38.0 33.0 40.0 2.0 41.0 54-55 32.20035847235627 37.0 33.0 40.0 2.0 41.0 56-57 31.841982627878117 37.0 32.5 40.0 2.0 41.0 58-59 31.44044533296567 36.0 31.5 40.0 2.0 41.0 60-61 30.931635185440506 36.0 31.0 39.0 2.0 40.5 62-63 30.459940714187233 35.0 30.5 39.0 2.0 40.0 64-65 30.092568592306634 35.0 30.0 39.0 2.0 40.0 66-67 29.689859368537157 35.0 29.0 38.5 2.0 40.0 68-69 29.129243071832345 35.0 28.5 38.0 2.0 40.0 70-71 28.596553150420515 35.0 27.5 37.0 2.0 39.5 72-73 28.11723424789742 34.0 26.0 37.0 2.0 39.0 74-75 27.653632979456777 34.0 26.0 36.0 2.0 39.0 76-77 26.318688818419965 32.5 24.0 35.0 2.0 37.0 78-79 26.842382462429338 34.0 25.0 35.0 2.0 37.0 80-81 26.614290638356543 34.0 24.0 35.0 2.0 37.0 82-83 26.237977388666756 33.5 22.5 35.0 2.0 36.5 84-85 25.821025782434855 33.0 20.0 35.0 2.0 36.0 86-87 25.426175375706606 33.0 18.5 35.0 2.0 35.5 88-89 25.010326761340135 33.0 13.5 35.0 2.0 35.0 90-91 24.552261133324144 32.0 4.5 35.0 2.0 35.0 92-93 24.092086033365504 32.0 2.0 34.0 2.0 35.0 94-95 23.594126568316558 31.5 2.0 34.0 2.0 35.0 96-97 22.938528884599478 31.0 2.0 34.0 2.0 35.0 98-99 22.217213566799945 31.0 2.0 34.0 2.0 35.0 100-101 19.6701778574383 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2159332438415973 1101 2 0.3278347427002686 1101 3 0.28757966883957664 1101 4 0.2416041556013795 1101 5 0.2595550978691108 1101 6 0.23249470885183854 1101 7 0.20176563460509556 1101 8 0.07057453206083864 1101 9 0.19365216672010632 1101 10-11 0.38318156344918464 1101 12-13 -0.050362819543686044 1101 14-15 0.2701983814873401 1101 16-17 0.5296003123059663 1101 18-19 0.565898330994159 1101 20-21 0.10601119390784675 1101 22-23 0.7041162220657924 1101 24-25 0.5259772434069561 1101 26-27 0.27900091935536153 1101 28-29 0.39594958285537984 1101 30-31 0.6847564849088386 1101 32-33 0.8423075704316645 1101 34-35 0.5140753459186911 1101 36-37 0.5571292879501257 1101 38-39 0.8770080336460992 1101 40-41 0.9074740788811653 1101 42-43 1.3391177500621367 1101 44-45 1.4862888002337442 1101 46-47 1.3287846798304912 1101 48-49 1.2093655097258917 1101 50-51 1.4515317643436525 1101 52-53 1.8043505381806035 1101 54-55 1.63934976461044 1101 56-57 1.3349120571225228 1101 58-59 1.6152156997789717 1101 60-61 2.0750824765991567 1101 62-63 2.055341763190224 1101 64-65 2.5127081516299867 1101 66-67 2.654775774049366 1101 68-69 2.8165270714129385 1101 70-71 2.884172439501416 1101 72-73 2.8911691145762397 1101 74-75 3.021581200068905 1101 76-77 3.3849306587446577 1101 78-79 3.150587998797228 1101 80-81 3.4439101443491396 1101 82-83 3.9165827146491416 1101 84-85 3.3928039395008938 1101 86-87 3.335595936906355 1101 88-89 3.444288501415297 1101 90-91 3.8464878601158716 1101 92-93 3.4783424133663097 1101 94-95 3.2966703824386165 1101 96-97 3.3441722198809707 1101 98-99 3.4001367630667616 1101 100-101 2.785191894188113 1102 1 -0.3553337701735124 1102 2 -0.40692837374223956 1102 3 -0.11364816750276674 1102 4 -0.22384275259361885 1102 5 -0.4624045935562293 1102 6 -0.02632026752038996 1102 7 -0.10216424947746106 1102 8 -0.2643339860537921 1102 9 0.03403344221588611 1102 10-11 -0.004435060808660296 1102 12-13 -0.18603065750160397 1102 14-15 -0.23527437589381606 1102 16-17 0.05706507259515092 1102 18-19 0.46044172935435057 1102 20-21 -0.06089780887901952 1102 22-23 -0.07986132145373404 1102 24-25 -0.0010028446418317571 1102 26-27 0.06828966284257376 1102 28-29 -0.9009929430947778 1102 30-31 -0.22174216204782482 1102 32-33 -0.2069068566453609 1102 34-35 0.23345011872857668 1102 36-37 0.584472880179213 1102 38-39 0.8440099319457133 1102 40-41 1.1888061197551991 1102 42-43 1.4547524687502928 1102 44-45 1.9446069875595597 1102 46-47 1.731264586127196 1102 48-49 2.3725183026496737 1102 50-51 2.95100670274989 1102 52-53 3.339085382075748 1102 54-55 3.3428717162239927 1102 56-57 3.239815324621219 1102 58-59 3.0869431524224495 1102 60-61 3.669755120890123 1102 62-63 3.6961592437023647 1102 64-65 4.398850564489962 1102 66-67 4.535465624204864 1102 68-69 4.518695979688719 1102 70-71 3.8364726131756406 1102 72-73 3.9863870151376517 1102 74-75 4.194891915364355 1102 76-77 4.531443038081466 1102 78-79 3.9471468258904068 1102 80-81 4.110314280218926 1102 82-83 4.52123152922162 1102 84-85 4.058178146859834 1102 86-87 3.795913397223817 1102 88-89 3.943743245144848 1102 90-91 4.0048949040191 1102 92-93 4.008432481624315 1102 94-95 3.576225291764519 1102 96-97 3.2817706689298056 1102 98-99 3.2925193310218503 1102 100-101 3.1881564727252147 1103 1 -0.09964962012836764 1103 2 -0.29070084357725534 1103 3 -0.2611487831146846 1103 4 -0.05418010792482164 1103 5 -0.10740172070060794 1103 6 -0.030178102208068935 1103 7 0.0022435318571822904 1103 8 -0.12553401888642668 1103 9 -0.20556271636574763 1103 10-11 -0.2046320566225006 1103 12-13 -0.4693763030733038 1103 14-15 -0.2097470016838443 1103 16-17 -0.15404877456892763 1103 18-19 0.09755902394517335 1103 20-21 0.10933940691347743 1103 22-23 0.1284207108864166 1103 24-25 0.014797861259836509 1103 26-27 -0.07174152816128299 1103 28-29 0.03863604812248411 1103 30-31 -0.07518548471055198 1103 32-33 0.33676054875560624 1103 34-35 -0.0727142086127941 1103 36-37 0.19503678069633423 1103 38-39 -0.021950833054695806 1103 40-41 -0.44958343946667156 1103 42-43 0.14079039044445807 1103 44-45 0.5103543404283144 1103 46-47 0.5834356441588824 1103 48-49 0.43235578110326145 1103 50-51 0.7410009570386471 1103 52-53 0.8756937708141557 1103 54-55 1.0028076925844935 1103 56-57 0.7014421817171659 1103 58-59 1.0949050665651114 1103 60-61 1.0060433914310423 1103 62-63 1.5125016880920903 1103 64-65 1.6812522717870095 1103 66-67 1.7449788803815025 1103 68-69 1.7241051564103813 1103 70-71 1.6817488177229585 1103 72-73 2.014910367349753 1103 74-75 1.697805811777389 1103 76-77 1.2988238145732964 1103 78-79 1.0817195912191444 1103 80-81 0.7176769987611529 1103 82-83 1.051930043544857 1103 84-85 0.9266834411223321 1103 86-87 1.2152682359334968 1103 88-89 1.2138737548715177 1103 90-91 0.4666448834986987 1103 92-93 1.2290677931239458 1103 94-95 1.6253100820460595 1103 96-97 1.18236986453023 1103 98-99 0.7988396133825155 1103 100-101 0.9095305185267399 1104 1 0.0208767801284111 1104 2 0.0352500471304964 1104 3 0.31371881438170846 1104 4 0.07774093203666155 1104 5 -0.12345050901708987 1104 6 0.12019904431736705 1104 7 0.11442456218939157 1104 8 0.20940419327855864 1104 9 0.3529351170478279 1104 10-11 0.23061108252049678 1104 12-13 0.30160748765049306 1104 14-15 0.3228320385986123 1104 16-17 -0.03191749548049927 1104 18-19 -0.17815915933199022 1104 20-21 -0.21476587313787832 1104 22-23 -0.595299398245345 1104 24-25 0.14198450866795298 1104 26-27 0.23896301364580808 1104 28-29 -0.264044099526366 1104 30-31 -0.474960801674122 1104 32-33 -0.06004574236941096 1104 34-35 -0.3338279945219611 1104 36-37 -0.31651834689153446 1104 38-39 -0.19848750453599706 1104 40-41 -0.2136662512143772 1104 42-43 -1.145680481004753 1104 44-45 -0.5207000649640747 1104 46-47 0.7120666825154842 1104 48-49 1.1302373410454862 1104 50-51 1.7954865933731092 1104 52-53 1.590025338780773 1104 54-55 1.40844081728358 1104 56-57 2.027954780015996 1104 58-59 1.4441267003317613 1104 60-61 1.8231674485647247 1104 62-63 0.09202343420025372 1104 64-65 0.6898383735363289 1104 66-67 0.5547204948896045 1104 68-69 1.3849942592831148 1104 70-71 1.4394121141440799 1104 72-73 0.8087636814245407 1104 74-75 1.4314761696653697 1104 76-77 1.1364903207520811 1104 78-79 1.8084889701310374 1104 80-81 1.943001986724564 1104 82-83 1.6668393678908693 1104 84-85 0.19028478922055214 1104 86-87 0.22128653155217748 1104 88-89 0.3941818916071931 1104 90-91 0.4390409675943516 1104 92-93 0.4582444902833096 1104 94-95 0.43842194210062146 1104 96-97 1.321191883467801 1104 98-99 0.8646396454800929 1104 100-101 0.6483364886759944 1105 1 -0.5222770161077968 1105 2 -0.6490458646151218 1105 3 -0.11879254188045962 1105 4 -0.06469904200616838 1105 5 -0.3369482377646591 1105 6 -0.1919359329701038 1105 7 -0.35540022690859274 1105 8 -0.16924603384669012 1105 9 -0.41734392253685826 1105 10-11 -0.4115044268889818 1105 12-13 -0.2780485798381491 1105 14-15 -0.5420284416090482 1105 16-17 -0.323286736233257 1105 18-19 0.3456889912195962 1105 20-21 0.10256053828327794 1105 22-23 -0.27667512829077623 1105 24-25 -1.1106112083988862 1105 26-27 -0.5764166099752117 1105 28-29 -1.1897416984880778 1105 30-31 -0.6771022682542664 1105 32-33 -1.281168390000822 1105 34-35 -0.9056969108100859 1105 36-37 0.32932850580152007 1105 38-39 0.550863566197286 1105 40-41 0.08127210050528078 1105 42-43 0.09555897389466139 1105 44-45 -1.5818616483514845 1105 46-47 -1.535662065051035 1105 48-49 -2.4529878374100598 1105 50-51 -1.3107755740506448 1105 52-53 -0.8689298850998206 1105 54-55 -0.8443171320999348 1105 56-57 -0.9877817547017216 1105 58-59 -1.2958148960342335 1105 60-61 -1.8655087357311864 1105 62-63 -1.9842257546381603 1105 64-65 -1.2739838198445952 1105 66-67 -2.4704255141954015 1105 68-69 -2.373604053500795 1105 70-71 -1.9387514160311348 1105 72-73 -1.4655711660770834 1105 74-75 -1.2304316823787609 1105 76-77 -1.3815174663991172 1105 78-79 -0.8621794270136718 1105 80-81 -2.015142075572644 1105 82-83 -1.2466908073200322 1105 84-85 -2.6932324074050165 1105 86-87 -1.671477907124011 1105 88-89 -2.5199972128704182 1105 90-91 -2.335131647590593 1105 92-93 -1.823877508418832 1105 94-95 -1.9123243265560923 1105 96-97 -1.8668353715614003 1105 98-99 -0.3991501014374954 1105 100-101 -1.9056868727776077 1106 1 0.05234108024943396 1106 2 -0.1067426918771659 1106 3 0.2636986449585521 1106 4 -0.03238411838689359 1106 5 0.22709406540808175 1106 6 -0.52683605047892 1106 7 -0.6620982292587669 1106 8 -0.4553799938936933 1106 9 0.14317311624105145 1106 10-11 0.09789627816390123 1106 12-13 0.17171640253553022 1106 14-15 -0.2614761501871925 1106 16-17 -0.2598319771263249 1106 18-19 -0.4331006680048475 1106 20-21 -0.7399776520809951 1106 22-23 -0.08924857129899522 1106 24-25 -1.123243405813696 1106 26-27 -0.20920156884712782 1106 28-29 -1.9370977501919526 1106 30-31 -1.820463020310676 1106 32-33 -1.7056689775448817 1106 34-35 -1.7491164172730649 1106 36-37 -2.7679100370891945 1106 38-39 -2.5316863750483023 1106 40-41 -1.7072120358049432 1106 42-43 -1.9546760437316593 1106 44-45 -3.1138827999378584 1106 46-47 -2.689304838259023 1106 48-49 -3.0484637481033623 1106 50-51 -1.7658284530165673 1106 52-53 -2.1189298850998206 1106 54-55 -1.480627355366682 1106 56-57 -1.6061718839614159 1106 58-59 -1.8777772932140238 1106 60-61 -2.257106855590173 1106 62-63 -2.193978986130521 1106 64-65 -1.8154056764838415 1106 66-67 -2.545924926651338 1106 68-69 -2.7325934777076064 1106 70-71 -2.8538512985223186 1106 72-73 -2.402410179003052 1106 74-75 -3.52596634747864 1106 76-77 -4.231399957585957 1106 78-79 -3.728219380010149 1106 80-81 -3.444049243610248 1106 82-83 -3.1236003255339 1106 84-85 -3.162680115983161 1106 86-87 -2.933816332505913 1106 88-89 -2.959186402059604 1106 90-91 -3.8915358779078666 1106 92-93 -3.9405050054811106 1106 94-95 -3.4393513535831204 1106 96-97 -3.466424090715339 1106 98-99 -3.8644850015550034 1106 100-101 -4.293172184175962 1107 1 0.6911617190700667 1107 2 -0.11083769597217241 1107 3 0.005713386973297929 1107 4 0.30750122149844117 1107 5 -0.019425181111166978 1107 6 0.3060877824449122 1107 7 0.5418329746724311 1107 8 0.44142590291220785 1107 9 0.6419446150125552 1107 10-11 0.236716916984534 1107 12-13 0.26180649262562383 1107 14-15 0.4813575926465532 1107 16-17 0.2643285470341965 1107 18-19 0.6786929437887679 1107 20-21 0.5814801693768175 1107 22-23 0.9426924606420357 1107 24-25 0.4918262092559189 1107 26-27 -0.9295127891583448 1107 28-29 -0.6172779303721398 1107 30-31 -0.5473262471738991 1107 32-33 -0.5865043583802674 1107 34-35 -0.6049722731289222 1107 36-37 -0.4628322320113867 1107 38-39 0.5215486781867469 1107 40-41 0.787464458871554 1107 42-43 -0.36458595364157276 1107 44-45 -0.03607772213278082 1107 46-47 -0.48987813883232434 1107 48-49 0.6112414116017959 1107 50-51 1.1256539384658275 1107 52-53 0.3659185997486638 1107 54-55 0.49889762415829964 1107 56-57 0.8676200898305595 1107 58-59 0.4805355650988332 1107 60-61 0.037323938840618354 1107 62-63 1.2568809647294295 1107 64-65 1.169033307955143 1107 66-67 0.547031666305255 1107 68-69 -0.9230111681252957 1107 70-71 -0.06556301023402966 1107 72-73 1.037393260800389 1107 74-75 0.5805037589914654 1107 76-77 0.3750701488841486 1107 78-79 1.556792905002137 1107 80-81 1.1673348677738637 1107 82-83 1.1462604443268702 1107 84-85 0.9134869601839135 1107 86-87 0.4928830941935125 1107 88-89 1.3057603628871597 1107 90-91 0.8816008952289032 1107 92-93 -0.7029947679708748 1107 94-95 0.04222112798936095 1107 96-97 0.603190978899729 1107 98-99 -0.13762177469177672 1107 100-101 0.6671062761024977 1108 1 -0.08635024265242208 1108 2 0.30862003927503423 1108 3 -0.2000282398209663 1108 4 -0.03380659349357984 1108 5 0.22140416498133675 1108 6 0.298910748952089 1108 7 0.2475746014666953 1108 8 -0.18297601096339378 1108 9 0.14672930400777062 1108 10-11 0.038152323683100064 1108 12-13 0.3758415803449182 1108 14-15 0.11014547143442854 1108 16-17 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0.17193720006129354 2312 34-35 0.06169439353774209 2312 36-37 -0.4312305004096615 2312 38-39 -0.13516127852320636 2312 40-41 -0.7454359740288794 2312 42-43 -1.2264907155463263 2312 44-45 -0.3914889775440358 2312 46-47 -0.5310036799578626 2312 48-49 -0.873174172813787 2312 50-51 -1.4812724684605847 2312 52-53 -1.5617870279569637 2312 54-55 -0.9188512935906203 2312 56-57 -2.0328128106023406 2312 58-59 -2.444572660009392 2312 60-61 -2.4540180525013753 2312 62-63 -1.6885735807251159 2312 64-65 -0.7655987266768918 2312 66-67 -1.0235311042575148 2312 68-69 -0.07236181747594728 2312 70-71 -0.28937253404355445 2312 72-73 -0.19507427166714564 2312 74-75 -1.6043447258570183 2312 76-77 -1.381206907392908 2312 78-79 -1.1509993027900691 2312 80-81 -2.462968162529169 2312 82-83 -0.8749516768852494 2312 84-85 -0.9267727800758259 2312 86-87 -1.6612294599190385 2312 88-89 -1.8271400700132752 2312 90-91 -0.8984856848576754 2312 92-93 -0.5428216077977126 2312 94-95 -0.8123243265560909 2312 96-97 -2.8050341293253744 2312 98-99 -3.414678051748055 2312 100-101 -3.6761837671875455 2313 1 0.21064223855059083 2313 2 0.15323156809709104 2313 3 0.11826749952740556 2313 4 0.17546658946380944 2313 5 0.20135403966805399 2313 6 -0.05321957686244616 2313 7 0.09594552878498774 2313 8 -0.0802191187328134 2313 9 0.11164158470952401 2313 10-11 0.2204831507507663 2313 12-13 0.38085411167323713 2313 14-15 0.30062166191061834 2313 16-17 0.3087008414064911 2313 18-19 0.17436393945976647 2313 20-21 0.6377572256538713 2313 22-23 -0.31704779909822633 2313 24-25 0.25481322224293024 2313 26-27 0.38217552252996256 2313 28-29 -0.08156364465784804 2313 30-31 0.20592050607285728 2313 32-33 0.5552705333946264 2313 34-35 -0.3549722731289293 2313 36-37 -0.9098019289810892 2313 38-39 -0.8756374689993933 2313 40-41 -0.6216264502193525 2313 42-43 0.5163664273108068 2313 44-45 -0.9343461204011803 2313 46-47 -2.150051299005483 2313 48-49 -1.3065075061471205 2313 50-51 -2.00746294465106 2313 52-53 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74-75 0.8922654436345034 2314 76-77 1.3647979352221071 2314 78-79 0.16619198050290862 2314 80-81 1.0438114984877807 2314 82-83 1.4826754417588184 2314 84-85 1.4165686242825295 2314 86-87 1.8298117023085645 2314 88-89 1.8655960074685645 2314 90-91 2.289407777612059 2314 92-93 1.4220694333645163 2314 94-95 -0.2767311062171096 2314 96-97 0.5990820934349124 2314 98-99 0.7342323598742198 2314 100-101 0.7061406880182659 2315 1 -0.1750720471636953 2315 2 -0.008673193807666735 2315 3 -0.37220869094878495 2315 4 -0.10072388672666222 2315 5 -0.031979293665280295 2315 6 0.18011375647088812 2315 7 -0.5802449474054896 2315 8 -0.018314356828049938 2315 9 -0.27883460576666863 2315 10-11 -0.5438025635349462 2315 12-13 0.035616016435149334 2315 14-15 -0.3327116714227145 2315 16-17 -0.019870587164930953 2315 18-19 0.2553163204121489 2315 20-21 -0.29557610767945164 2315 22-23 -0.4741906562410847 2315 24-25 -0.41899630156659384 2315 26-27 -0.6773482869938476 2315 28-29 -0.6363255494197517 2315 30-31 -0.5631271129747617 2315 32-33 -0.04949137136727444 2315 34-35 0.22598010782345312 2315 36-37 -0.10980192898109209 2315 38-39 0.6219815786196463 2315 40-41 0.34027831168540246 2315 42-43 0.3163664273108111 2315 44-45 0.35136816531311155 2315 46-47 -0.10957510852929175 2315 48-49 0.4768258271862109 2315 50-51 0.7330132458251306 2315 52-53 1.05964154347161 2315 54-55 1.081148706409376 2315 56-57 0.9600443322548031 2315 58-59 1.6839987685620343 2315 60-61 1.8816962332129137 2315 62-63 1.6971407049891702 2315 64-65 1.848686987608822 2315 66-67 1.6836117528853407 2315 68-69 1.1347810396669082 2315 70-71 1.5891988945278719 2315 72-73 1.6192114426185675 2315 74-75 2.3599409884286935 2315 76-77 1.9402216640356649 2315 78-79 2.399000697209928 2315 80-81 3.101317551756548 2315 82-83 3.0679054659718936 2315 84-85 2.644655791352747 2315 86-87 2.8244848257952455 2315 88-89 2.3228599299867234 2315 90-91 2.4300857437137537 2315 92-93 1.9428926779165714 2315 94-95 1.9376756734439091 2315 96-97 1.3735372992460526 2315 98-99 1.5138933768233755 2315 100-101 0.823816232812451 2316 1 -0.35045931050276735 2316 2 -0.22657336592470756 2316 3 -0.3458747838919862 2316 4 -0.255112871236129 2316 5 0.059070620276202135 2316 6 -0.2930359853535549 2316 7 -0.1306752400044573 2316 8 0.0887424417950129 2316 9 0.0734889742677538 2316 10-11 -0.5579161607810761 2316 12-13 -0.2100810575751808 2316 14-15 -0.44708344422821966 2316 16-17 -0.8371683496434201 2316 18-19 -0.44726543518165585 2316 20-21 -0.10435407669838526 2316 22-23 -0.30758136192094554 2316 24-25 -0.5017845976078945 2316 26-27 0.24993226378067845 2316 28-29 -0.37229801069342017 2316 30-31 -0.24703416977337866 2316 32-33 -0.771608410954201 2316 34-35 -0.10095620887189938 2316 36-37 0.23778843246468995 2316 38-39 0.5923774478106978 2316 40-41 0.717730979499521 2316 42-43 1.326177098567264 2316 44-45 1.2291650672063952 2316 46-47 2.0464489878562517 2316 48-49 1.4020409734856898 2316 50-51 1.359863504000689 2316 52-53 0.9352869823700551 2316 54-55 1.7876891539136857 2316 56-57 1.91727324791745 2316 58-59 1.7580090955844128 2316 60-61 2.3334174036087774 2316 62-63 2.4282938891544 2316 64-65 2.34610523201502 2316 66-67 2.8513398079627947 2316 68-69 2.8519066851058064 2316 70-71 2.279818515870385 2316 72-73 2.032464454666762 2316 74-75 1.1739685271550293 2316 76-77 1.6552819049995193 2316 78-79 2.3963328830963313 2316 80-81 2.5888390663864946 2316 82-83 2.38649410624728 2316 84-85 2.1999053610601464 2316 86-87 3.151335083970803 2316 88-89 2.802291943756085 2316 90-91 2.344543575039058 2316 92-93 1.9132885471076229 2316 94-95 1.7503262758535456 2316 96-97 1.9562395367675656 2316 98-99 1.8693150635703617 2316 100-101 2.005916060695416 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 1.0 6 11.0 7 17.0 8 27.0 9 84.0 10 159.0 11 289.0 12 365.0 13 486.0 14 669.0 15 1031.0 16 1399.0 17 2032.0 18 1787.0 19 1068.0 20 828.0 21 863.0 22 869.0 23 903.0 24 978.0 25 958.0 26 1003.0 27 1096.0 28 1282.0 29 1386.0 30 1648.0 31 1899.0 32 2423.0 33 3257.0 34 4726.0 35 6949.0 36 9887.0 37 13590.0 38 8198.0 39 360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03072552507826 21.53958600526802 17.19415828885855 17.235530180795166 2 15.61858015414524 25.424313033407326 39.297384494478074 19.659722317969365 3 18.692954639459533 30.405349510547357 32.600303322763 18.301392527230114 4 11.491796498000827 21.409072108093202 38.982490004136224 28.116641389769754 5 11.325281270681668 40.31960070593426 33.88622325170969 14.468894771674387 6 25.3977664414725 41.26568316558665 17.42589273404109 15.910657658899765 7 23.15625473948352 38.87134801251913 22.33313571123275 15.639261536764604 8 25.33434440920998 34.07279746311871 19.946229146560043 20.646628981111263 9 26.670343306218115 18.655728664001103 21.414587067420378 33.2593409623604 10-11 23.05735557700262 29.71804770439818 26.725492899489865 20.499103819109337 12-13 26.771555825209248 28.915072875442977 27.310710001241016 17.002661298106755 14-15 22.653788615195825 29.166580491268707 29.508524470366005 18.671106423169462 16-17 17.85881704122432 32.82365917551358 29.522956018199366 19.79456776506273 18-19 17.64316588422642 29.78787941458303 35.38698047001565 17.1819742311749 20-21 19.695705825985645 27.82699214791427 35.53430720337523 16.94299482272486 22-23 19.044073545531635 28.464078647115194 35.33604958187697 17.155798225476204 24-25 19.217962594014768 28.978263717021584 35.70665324665476 16.097120442308885 26-27 18.540170138841013 29.195218464338403 36.16967006300928 16.094941333811303 28-29 17.296291189852475 29.633255204742863 36.61726182269406 16.4531917827106 30-31 18.179374052116366 29.701502826416654 35.37294912450021 16.746173996966775 32-33 17.78850130980284 30.73555770026196 34.66703433062181 16.80890665931339 34-35 17.892596167103267 29.90900317110161 35.231628291741345 16.96677237005377 36-37 18.598157901993766 29.872735294928717 35.01365027714199 16.515456525935527 38-39 17.66762039367093 30.791133786066393 35.2966320796994 16.244613740563274 40-41 17.068580410324287 31.16520516214428 34.6369677917494 17.129246635782042 42-43 17.50034468495795 31.56900592858128 33.38067006755825 17.549979318902523 44-45 18.31405368886928 30.545023369962358 33.155703235947 17.985219705221358 46-47 18.47649248586792 31.34082448641941 32.36798566110575 17.81469736660692 48-49 18.77774713911485 31.250517027436924 31.90128222804357 18.07045360540466 50-51 18.23252030278379 31.672342713747987 31.759896314475988 18.335240668992235 52-53 18.08975596304977 32.54101750999586 30.685233696401486 18.683992830552874 54-55 17.79470563904591 31.663449607059146 30.685233696401486 19.85661105749345 56-57 18.56404246518682 31.435268164897284 30.63215221287743 19.368537157038467 58-59 19.091410450847928 30.996139528470977 30.509444367847788 19.40300565283331 60-61 18.081417393402504 31.1978215159767 31.46668505049809 19.254076040122712 62-63 17.894664276850957 30.61216048531642 31.999862126016822 19.493313111815798 64-65 17.80435681786847 30.739004549841443 31.879911760650764 19.576726871639323 66-67 18.093892182545154 30.14132083275886 31.07748517854681 20.68730180614918 68-69 18.000827243899074 30.038604715290223 30.853439955880326 21.107128084930373 70-71 18.023576451123674 30.133048393768096 31.054046601406316 20.789328553701917 72-73 17.38590927891907 29.34371984006618 31.673790155797597 21.59658072521715 74-75 17.01584937919243 30.245496473702715 30.679820479410974 22.05883366769388 76-77 17.454846270508757 29.57190128222804 30.585275058596444 22.38797738866676 78-79 17.38797738866676 29.356128498552327 30.171653109058322 23.084241003722596 80-81 17.165665910642023 29.518740908746217 29.356046243890333 23.959546936721427 82-83 16.873018061491795 28.537157038466844 29.8897008134565 24.700124086584864 84-85 16.39838132596152 28.142730099202385 29.885493288845073 25.573395285991023 86-87 16.326347718185577 29.157589962774022 28.89287191506963 25.62319040397077 88-89 16.087690875874667 27.912860639067937 28.931095101857917 27.06835338319948 90-91 16.044616636104177 28.168043127576553 29.166264528671288 26.62107570764797 92-93 15.924445057217703 28.936302219771132 28.445470839652558 26.693781883358607 94-95 15.567351440783126 29.525713497862956 27.528608851509723 27.3783262098442 96-97 15.602509306493864 30.7045360540466 26.682062594788363 27.01089204467117 98-99 16.025093064938645 30.554253412381083 25.119950365366055 28.300703157314217 100-101 15.245625887576002 28.83260488907885 25.102372843965863 30.81939637937928 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 545.0 1 379.0 2 210.5 3 194.0 4 172.5 5 193.5 6 277.0 7 413.0 8 526.5 9 567.5 10 499.5 11 450.0 12 497.0 13 500.0 14 449.5 15 414.5 16 399.0 17 394.0 18 391.0 19 402.5 20 418.5 21 412.5 22 441.0 23 487.5 24 505.0 25 557.0 26 647.5 27 719.0 28 777.5 29 936.5 30 1135.5 31 1281.5 32 1433.5 33 1572.0 34 1692.0 35 1875.5 36 2247.5 37 2458.5 38 2512.0 39 2519.5 40 2396.5 41 2264.0 42 2153.5 43 2189.0 44 2224.0 45 2170.5 46 2177.0 47 2593.0 48 3313.0 49 3037.0 50 2224.5 51 1807.5 52 1527.5 53 1414.0 54 1308.5 55 1176.0 56 1051.5 57 972.0 58 914.5 59 801.0 60 648.5 61 526.5 62 404.5 63 290.5 64 209.0 65 159.5 66 113.5 67 79.5 68 51.5 69 27.0 70 21.0 71 14.5 72 10.5 73 6.0 74 3.0 75 5.0 76 4.5 77 1.5 78 1.0 79 2.0 80 1.0 81 0.5 82 1.5 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02343857714049359 2 0.0013787398317937406 3 0.0 4 0.0 5 0.002757479663587481 6 0.0 7 0.0013787398317937406 8 0.0 9 0.0 10-11 0.0 12-13 0.012408658486143666 14-15 0.004825589411278091 16-17 0.0 18-19 6.893699158968703E-4 20-21 0.0020681097476906106 22-23 0.004825589411278091 24-25 0.0020681097476906106 26-27 0.0013787398317937406 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.005514959327174962 38-39 0.010340548738453054 40-41 0.002757479663587481 42-43 0.0 44-45 0.0013787398317937406 46-47 0.0 48-49 0.0 50-51 0.004136219495381221 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0034468495794843512 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.004825589411278091 76-77 0.0 78-79 0.0 80-81 0.0020681097476906106 82-83 0.0 84-85 0.0020681097476906106 86-87 0.0 88-89 0.0034468495794843512 90-91 0.0013787398317937406 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0013787398317937406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 72530.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.63201433889425 #Duplication Level Percentage of deduplicated Percentage of total 1 86.16901842516566 53.96939197573418 2 7.581394324960926 9.496759961395284 3 2.31360204283796 4.347166689645664 4 1.1336870143306845 2.8402040534951056 5 0.664802870539547 2.081897146008548 6 0.4248574636229555 1.5965807252171516 7 0.3302001012613644 1.4476768233834276 8 0.23554273889977326 1.1802012960154418 9 0.1673013846390913 0.9430580449469185 >10 0.8915402734056839 9.681511098855646 >50 0.041825346159772826 1.7303184889011443 >100 0.03302001012613644 2.9711843375155107 >500 0.006604002025227288 2.7822969805597686 >1k 0.006604002025227288 4.93175237832621 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1269 1.7496208465462566 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 1.6986074727698883 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1076 1.483524059010065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 737 1.0161312560319868 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 641 0.8837722321797877 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 640 0.8823934923479939 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 262 0.36122983592996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 214 0.29505032400386044 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 208 0.28677788501309803 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 148 0.2040534951054736 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 143 0.1971597959465049 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 141 0.19440231628291743 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 135 0.18612987729215497 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.18612987729215497 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 124 0.17096373914242383 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 114 0.15717634082448642 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 110 0.15166138149731145 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 109 0.15028264166551772 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 105 0.14476768233834275 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 104 0.14338894250654902 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 103 0.14201020267475525 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.13511650351578655 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 98 0.13511650351578655 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 88 0.12132910519784916 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 80 0.11029918654349924 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 77 0.10616296704811802 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 73 0.10064800772094307 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0013787398317937406 0.0 14-15 0.0 0.0 0.0 0.0013787398317937406 0.0 16-17 0.0 0.0 0.0 0.0013787398317937406 0.0 18-19 0.0 0.0 0.0 0.0013787398317937406 0.0 20-21 0.0 0.0 0.0 0.002068109747690611 0.0 22-23 0.0 0.0 0.0 0.0068936991589687024 0.0 24-25 0.0 0.0 0.0 0.013098028402040534 0.0 26-27 0.0 0.0 0.0 0.02205983730869985 0.0 28-29 0.0 0.0 0.0 0.041362194953812215 0.0 30-31 0.0 0.0 0.0 0.08272438990762443 0.0 32-33 0.0 0.0 0.0 0.11029918654349924 0.0 34-35 0.0 0.0 0.0 0.13442713359988973 0.0 36-37 0.0 0.0 0.0 0.164759409899352 0.0 38-39 0.0 0.0 0.0 0.2109471942644423 0.0 40-41 0.0 0.0 0.0 0.31917827106025093 0.0 42-43 0.0 0.0 0.0 0.5080656280159934 0.0 44-45 0.0 0.0 0.0 0.674893147663036 0.0 46-47 0.0 0.0 0.0 1.1464221701364954 0.0 48-49 0.0 0.0 0.0 1.5531504205156488 0.0 50-51 0.0 0.0 0.0 1.8027023300703158 0.0 52-53 0.0 0.0 0.0 2.0943058044946916 0.0 54-55 0.0 0.0 0.0 2.418309664966221 0.0 56-57 0.0 0.0 0.0 3.145594926237419 0.0 58-59 0.0 0.0 0.0 3.9673238659864882 0.0 60-61 0.0 0.0 0.0 4.810423273128361 0.0 62-63 0.0 0.0 0.0 5.787260443954226 0.0 64-65 0.0 0.0 0.0 6.855783813594375 0.0 66-67 0.0 0.0 0.0 8.098028402040534 0.0 68-69 0.0 0.0 0.0 9.360954087963602 0.0 70-71 0.0 0.0 0.0 10.443264855921687 0.0 72-73 0.0 0.0 0.0 11.42079139666345 0.0 74-75 0.0 0.0 0.0 12.500344684957948 0.0 76-77 0.0 0.0 0.0 13.841169171377361 0.0 78-79 0.0 0.0 0.0 14.908313801185717 0.0 80-81 0.0 0.0 0.0 16.2332827795395 0.0 82-83 0.0 0.0 0.0 17.979456776506275 0.0 84-85 0.0 0.0 0.0 20.358472356266375 0.0 86-87 0.0 0.0 0.0 22.461739969667725 0.0 88-89 0.0 0.0 0.0 24.144491934371985 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGTCC 20 0.002066699 71.245865 8 CTATGGC 30 1.2934256E-4 63.32965 9 GTATAGG 60 2.2990207E-8 55.45169 1 GTTATTC 45 1.5023128E-5 52.77471 3 GGTTATT 45 9.568163E-4 42.219772 2 GGCCATA 50 0.0016008634 38.024017 1 AATGCTC 25 0.0016116219 37.997795 16-17 CACTAAA 25 0.0016116219 37.997795 76-77 TGTCCTA 25 0.0016116219 37.997795 60-61 TTCTATG 65 1.3163975E-4 36.53634 7 TATTCTA 65 1.3163975E-4 36.53634 5 GGGTTAT 55 0.0025558388 34.567287 1 TACCCTA 55 0.0025645122 34.54345 5 TTATTCT 90 2.3830964E-5 31.664827 4 AGCTTTA 30 0.0039407946 31.664825 46-47 CCACTAA 30 0.0039407946 31.664825 76-77 CTAACCA 40 4.466994E-4 29.696016 38-39 TCCACTA 40 4.4760332E-4 29.685776 74-75 GAAAAGT 65 0.005810148 29.22907 7 TCCAAGA 65 0.005810148 29.22907 2 >>END_MODULE