FastQCFastQC Report
Thu 26 May 2016
SRR936927_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936927_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206298
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23111.12022414177549No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.7707297210830933No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.5288466199381477No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA7160.34707074232421065No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6520.316047659211432No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5960.28890246148775073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4280.20746686831670688No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT3800.18419955598212295No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.17838272789847695No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA3640.17644378520392828No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG3350.1623864506684505No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC3070.14881385180660986No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.1386344026602294No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2690.13039389620839756No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTA652.5602785E-643.8925065
GTGTAGC953.452075E-530.0317171
TATCCAG804.4533127E-429.718895
GTATCAA23900.029.4453221
ATGGGAG3000.028.530135
TAGGCTG700.00833277427.1715535
CGATTCT350.00838694227.14185172-73
GAGTACT12500.026.6151812-13
GTACATG14200.026.4539951
GTCCTAA908.8676024E-426.4167881
TATCAAC26950.025.9364832
ATCAACG27400.025.5167263
TACATGG14000.025.4733312
TCAACGC27550.025.2051564
AACGCAG27950.025.0085246
CAACGCA28050.024.9193675
ACATGGG13450.024.7533453
GTACTTT13450.024.5556314-15
ACGCAGA29550.023.6544257
CGCAGAG29650.023.419978
Produced by FastQC (version 0.11.5)