##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936927_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206298 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.95709119816964 33.0 31.0 34.0 30.0 34.0 2 32.0381826290124 33.0 31.0 34.0 30.0 34.0 3 32.0232430755509 34.0 31.0 34.0 30.0 34.0 4 35.5927977973611 37.0 35.0 37.0 33.0 37.0 5 35.61122260031605 37.0 35.0 37.0 33.0 37.0 6 35.7052273895045 37.0 35.0 37.0 35.0 37.0 7 35.65785901947668 37.0 35.0 37.0 33.0 37.0 8 35.62591493858399 37.0 35.0 37.0 33.0 37.0 9 37.27091876799581 39.0 37.0 39.0 34.0 39.0 10-11 37.16351346110966 39.0 37.0 39.0 34.0 39.0 12-13 37.07591687752668 39.0 37.0 39.0 33.5 39.0 14-15 38.22013543514721 40.0 38.0 41.0 33.5 41.0 16-17 38.16114552734393 40.0 38.0 41.0 33.0 41.0 18-19 38.19270181969772 40.0 38.0 41.0 33.5 41.0 20-21 38.1711359295776 40.0 38.0 41.0 34.0 41.0 22-23 38.04952059641877 40.0 38.0 41.0 33.5 41.0 24-25 37.889911681160264 40.0 38.0 41.0 33.0 41.0 26-27 37.806781936809855 40.0 37.5 41.0 33.0 41.0 28-29 37.827029345897685 40.0 38.0 41.0 33.0 41.0 30-31 37.629283851515765 40.0 37.0 41.0 33.0 41.0 32-33 37.37344036297007 40.0 36.5 41.0 33.0 41.0 34-35 37.15166894492433 40.0 36.0 41.0 32.0 41.0 36-37 36.96737486548585 39.0 36.0 41.0 31.5 41.0 38-39 36.762002055279254 39.0 35.0 41.0 31.0 41.0 40-41 36.48903043170559 39.0 35.0 41.0 30.5 41.0 42-43 36.24914928889277 39.0 35.0 41.0 30.0 41.0 44-45 35.904974842218536 39.0 35.0 41.0 29.5 41.0 46-47 35.38458443610699 39.0 35.0 40.0 26.5 41.0 48-49 34.90553228824322 38.5 35.0 40.0 24.0 41.0 50-51 34.2145900590408 38.0 34.0 39.5 23.0 40.5 52-53 34.469095677127264 38.5 34.5 40.0 23.0 40.5 54-55 34.75402088241282 39.0 35.0 40.5 23.0 41.0 56-57 34.62794113369979 39.0 35.0 41.0 22.5 41.0 58-59 34.393888937362455 38.5 34.5 41.0 21.5 41.0 60-61 34.06330163162028 38.0 34.0 40.0 19.5 41.0 62-63 33.761398559365574 38.0 34.0 40.0 18.0 41.0 64-65 33.40387691591775 37.5 34.0 40.0 14.0 41.0 66-67 33.00300536117655 37.0 33.0 40.0 9.0 41.0 68-69 32.566886251926825 36.0 33.0 39.0 7.0 41.0 70-71 32.164218751514795 36.0 32.0 39.0 2.0 41.0 72-73 31.7498933581518 35.0 32.0 39.0 2.0 40.0 74-75 31.300053805659772 35.0 31.5 37.5 2.0 40.0 76-77 30.84310560451386 35.0 31.0 37.0 2.0 39.0 78-79 30.401048483262077 35.0 31.0 37.0 2.0 39.0 80-81 29.98512830953281 35.0 30.5 36.0 2.0 39.0 82-83 29.55529622197016 34.5 30.0 36.0 2.0 37.0 84-85 29.195566607528914 34.0 30.0 35.0 2.0 37.0 86-87 28.849428981376455 34.0 29.0 35.0 2.0 36.5 88-89 28.446698465326858 34.0 29.0 35.0 2.0 36.0 90-91 28.063716080621237 34.0 29.0 35.0 2.0 36.0 92-93 27.714854239982937 34.0 27.5 35.0 2.0 35.5 94-95 27.343830284345948 34.0 27.0 35.0 2.0 35.0 96-97 26.901535642614085 34.0 25.5 35.0 2.0 35.0 98-99 26.322695808975368 34.0 25.0 35.0 2.0 35.0 100-101 24.3811403891458 31.5 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3164172839233039 1101 2 0.14082915847944122 1101 3 0.3231185051854162 1101 4 0.3253357220331168 1101 5 0.23496079477629195 1101 6 0.18931791945847465 1101 7 0.10701888112225078 1101 8 -0.01894815549668749 1101 9 0.1347059322754589 1101 10-11 0.14439145852850288 1101 12-13 0.23713986853102398 1101 14-15 0.36690438080571397 1101 16-17 0.12996871530643261 1101 18-19 0.34994867614157243 1101 20-21 0.30569860325995535 1101 22-23 0.28320410690897546 1101 24-25 0.31538675374378755 1101 26-27 0.5152961452828748 1101 28-29 0.20656972606945345 1101 30-31 0.31094762084327243 1101 32-33 0.4304720671030893 1101 34-35 0.5486873675115973 1101 36-37 0.25906058784784136 1101 38-39 0.4911320019259833 1101 40-41 0.9327731212608157 1101 42-43 0.9255603966672439 1101 44-45 0.9507815557846442 1101 46-47 0.8815667961038827 1101 48-49 0.9559181613646643 1101 50-51 1.2509693253359089 1101 52-53 1.4687249159541977 1101 54-55 1.383531281386034 1101 56-57 1.3709015736162016 1101 58-59 1.4638988175477223 1101 60-61 1.3417088665114036 1101 62-63 1.4594995967898967 1101 64-65 1.5547537635227329 1101 66-67 1.5998812098276858 1101 68-69 1.63987684638235 1101 70-71 1.8703736304095742 1101 72-73 1.9561214029570841 1101 74-75 1.9678589093925503 1101 76-77 1.796016463492009 1101 78-79 1.7872129661782488 1101 80-81 1.4467547932240912 1101 82-83 1.1789738245700754 1101 84-85 1.3535918519559687 1101 86-87 1.5969806969354856 1101 88-89 1.4805796127057498 1101 90-91 1.3663186326228818 1101 92-93 1.1598245087686578 1101 94-95 1.5770643447353265 1101 96-97 1.1705693229180874 1101 98-99 1.443539528799537 1101 100-101 1.671538618654882 1102 1 0.5642324544943342 1102 2 0.4616132554089347 1102 3 0.4648963136759541 1102 4 0.4090295464635858 1102 5 0.5221282331012134 1102 6 0.19013334340982624 1102 7 0.37378689991846414 1102 8 0.3946422436924948 1102 9 0.376638072417812 1102 10-11 0.6439952638402744 1102 12-13 0.671518971564673 1102 14-15 0.7518121273332525 1102 16-17 0.6411520014188881 1102 18-19 0.6356557224180364 1102 20-21 0.4598943510774234 1102 22-23 0.4597295716315202 1102 24-25 0.49856460369657185 1102 26-27 0.4523265278871591 1102 28-29 0.5219821601329855 1102 30-31 0.5481323350993748 1102 32-33 0.7854486870095698 1102 34-35 0.8361335241927748 1102 36-37 0.7844999723640029 1102 38-39 0.7856244350681223 1102 40-41 0.78513301345901 1102 42-43 1.0898360744596118 1102 44-45 1.0912786419109182 1102 46-47 1.1127993762066097 1102 48-49 1.5593295819068942 1102 50-51 1.5638779011081425 1102 52-53 1.623418210589442 1102 54-55 1.6009200819757226 1102 56-57 2.0518819251238156 1102 58-59 1.91174393306612 1102 60-61 1.9364178246575818 1102 62-63 2.109394789474109 1102 64-65 2.2308070194598955 1102 66-67 2.222685438975631 1102 68-69 2.5508113590859267 1102 70-71 2.6037530328926977 1102 72-73 2.5631331275557123 1102 74-75 2.543221128082802 1102 76-77 2.698955675248854 1102 78-79 2.713350298313788 1102 80-81 2.5795099587275168 1102 82-83 2.262909512036966 1102 84-85 2.434542258516217 1102 86-87 2.5067496601492714 1102 88-89 2.359376286513136 1102 90-91 2.4196482804242336 1102 92-93 2.412619754432402 1102 94-95 2.54916717107767 1102 96-97 2.1683211484081504 1102 98-99 2.499891312827362 1102 100-101 2.9256376212577884 1103 1 0.08084372717124921 1103 2 0.2976608942401171 1103 3 0.37283842695666536 1103 4 0.2276765482638936 1103 5 0.10804431120673996 1103 6 0.024325283584317958 1103 7 0.08637518408963274 1103 8 0.2964356241107211 1103 9 0.16859901965504065 1103 10-11 0.18812350092655805 1103 12-13 -0.03898165161771061 1103 14-15 0.11632189052394182 1103 16-17 0.2910619861855466 1103 18-19 0.5648483550173964 1103 20-21 0.40646338583715647 1103 22-23 0.26664689610181114 1103 24-25 0.22274061809901013 1103 26-27 0.41684321532657265 1103 28-29 0.3385415009445083 1103 30-31 0.7614268926398822 1103 32-33 0.6225972813870797 1103 34-35 0.7038561387890852 1103 36-37 0.9027938374796349 1103 38-39 1.116762781557533 1103 40-41 0.9484067982533588 1103 42-43 1.2386866491722515 1103 44-45 1.4749443391827626 1103 46-47 1.5795802929771412 1103 48-49 2.0133975780293127 1103 50-51 1.9904150843237645 1103 52-53 2.0999934771730224 1103 54-55 1.9969871087033226 1103 56-57 2.1687563192520543 1103 58-59 2.2700080239122613 1103 60-61 1.8828483744429292 1103 62-63 2.0990014921468756 1103 64-65 2.106447512466403 1103 66-67 2.3541457630304734 1103 68-69 2.4355676255310144 1103 70-71 2.338810504157067 1103 72-73 2.142273828289678 1103 74-75 2.6210116003165673 1103 76-77 2.423252864003878 1103 78-79 2.442674491638808 1103 80-81 2.6454322686568865 1103 82-83 2.6904576355655614 1103 84-85 2.2166915453185965 1103 86-87 2.401158329308668 1103 88-89 2.6332614824693756 1103 90-91 2.511373803205011 1103 92-93 2.264084233036474 1103 94-95 2.5634588204337128 1103 96-97 2.4494041009078025 1103 98-99 2.476186147337817 1103 100-101 2.5190076531093304 1104 1 0.4253818797816926 1104 2 0.5186247496618037 1104 3 0.5381530186568035 1104 4 0.29515981466282426 1104 5 0.3682585013004527 1104 6 0.31565058478913954 1104 7 0.209802225588966 1104 8 0.5043740444587783 1104 9 0.1248373061342889 1104 10-11 0.28805656652226475 1104 12-13 0.4667680137102721 1104 14-15 0.8003178744596866 1104 16-17 0.7344087063997264 1104 18-19 0.3610197070923604 1104 20-21 0.3392813242575059 1104 22-23 0.273139533317341 1104 24-25 0.5609400826237732 1104 26-27 0.43041081907490053 1104 28-29 0.521292504960563 1104 30-31 0.31449248835607335 1104 32-33 0.32767090923179154 1104 34-35 0.4468614935414337 1104 36-37 0.39990227121457167 1104 38-39 0.18478152319072905 1104 40-41 0.09609086786513643 1104 42-43 0.4486866491722523 1104 44-45 0.5310487568534441 1104 46-47 1.014178686551439 1104 48-49 0.9878353290333308 1104 50-51 1.241425793828455 1104 52-53 1.3636480956469157 1104 54-55 1.6136403884891308 1104 56-57 1.5281271358517898 1104 58-59 1.3115140480086467 1104 60-61 1.348678361056045 1104 62-63 1.5675557090143428 1104 64-65 1.5446001229081716 1104 66-67 1.352800381504366 1104 68-69 1.5647577192391822 1104 70-71 1.7910327263792851 1104 72-73 1.9422135873258206 1104 74-75 1.5189299403433374 1104 76-77 1.6634001196933 1104 78-79 2.0807066201528635 1104 80-81 2.0198164721374745 1104 82-83 1.7655915043741324 1104 84-85 1.458833446255678 1104 86-87 1.6709642195362413 1104 88-89 1.7572306926434074 1104 90-91 1.6777325716119726 1104 92-93 1.6707040456201412 1104 94-95 1.7326154469397395 1104 96-97 1.2508498840403348 1104 98-99 1.1844123856242987 1104 100-101 1.2619594603382502 1105 1 0.7510961654959765 1105 2 0.5042332152702755 1105 3 0.512332912836694 1105 4 0.4807682802712847 1105 5 0.5134436864856369 1105 6 0.5567087858473414 1105 7 0.4986911144778574 1105 8 0.39876557885031616 1105 9 0.6871653484623295 1105 10-11 0.6436121220778261 1105 12-13 0.4110008179430693 1105 14-15 0.7905824247242421 1105 16-17 0.7309166429076654 1105 18-19 0.7002789663516182 1105 20-21 0.6340961390723194 1105 22-23 0.8144093745871857 1105 24-25 0.8804109820946735 1105 26-27 0.8371833058473825 1105 28-29 0.8801284837965468 1105 30-31 0.7368205306841205 1105 32-33 0.9187820203429027 1105 34-35 1.0272847739647162 1105 36-37 1.1481562394685412 1105 38-39 1.4852048036140104 1105 40-41 1.1128104445847171 1105 42-43 1.14477130525691 1105 44-45 1.2563397621444565 1105 46-47 1.5684644008371578 1105 48-49 1.9224385036364993 1105 50-51 1.7252353176379742 1105 52-53 1.917087249086073 1105 54-55 1.885174780023526 1105 56-57 2.205904913629567 1105 58-59 1.8561701326647295 1105 60-61 2.141270953648643 1105 62-63 1.9849102063688377 1105 64-65 2.231901710209762 1105 66-67 2.149414138118118 1105 68-69 1.9903661848476446 1105 70-71 2.2422496575962185 1105 72-73 2.245599830712063 1105 74-75 2.1171309985443933 1105 76-77 2.3053048815980617 1105 78-79 2.6768970963433425 1105 80-81 2.916747689068689 1105 82-83 2.530141768924395 1105 84-85 2.7362937637159988 1105 86-87 2.771281679853704 1105 88-89 2.8167015921143026 1105 90-91 2.536251090130488 1105 92-93 2.5784289133450073 1105 94-95 2.8288059231302114 1105 96-97 2.882278455468903 1105 98-99 2.390549951761866 1105 100-101 2.8211128994916876 1106 1 0.5979576373574496 1106 2 0.6863520223890802 1106 3 0.7330520085557914 1106 4 0.3459678954708991 1106 5 0.5870968851388341 1106 6 0.5829233120618653 1106 7 0.8989436397303763 1106 8 0.738111418196155 1106 9 0.5400393263363057 1106 10-11 0.5987888897545943 1106 12-13 0.6888387207809714 1106 14-15 0.5774390865809025 1106 16-17 0.96233799932903 1106 18-19 1.2560449596176113 1106 20-21 1.1532459707221534 1106 22-23 1.0059678161456276 1106 24-25 1.436116850300543 1106 26-27 1.4255370817011581 1106 28-29 1.1891712928393545 1106 30-31 1.449542993406581 1106 32-33 1.3771153536762384 1106 34-35 1.4053210895010295 1106 36-37 1.5264931803054793 1106 38-39 1.6770037454129536 1106 40-41 1.7267474335217017 1106 42-43 1.4716411946267982 1106 44-45 1.2472608780655676 1106 46-47 1.6287998986726535 1106 48-49 1.9107141169121178 1106 50-51 0.8706177130203656 1106 52-53 1.8753905198893435 1106 54-55 2.064347459196206 1106 56-57 2.198329156053802 1106 58-59 2.351059502554101 1106 60-61 2.462188462066152 1106 62-63 2.7432375271961646 1106 64-65 2.4476304259384776 1106 66-67 2.402270078474068 1106 68-69 2.4824597394411967 1106 70-71 2.4524468677934266 1106 72-73 1.4056226782349164 1106 74-75 1.3114551928685962 1106 76-77 1.193652644945825 1106 78-79 1.2707823777286222 1106 80-81 0.6360538458748515 1106 82-83 1.1986723124549385 1106 84-85 1.2742627391849695 1106 86-87 1.2869743205463458 1106 88-89 0.785917561330276 1106 90-91 0.41861640999580985 1106 92-93 1.3918909143070124 1106 94-95 0.8412518105761038 1106 96-97 0.8506226113130602 1106 98-99 1.1002962240081366 1106 100-101 1.404686733065521 1107 1 0.37014378454359687 1107 2 0.4386247496618054 1107 3 0.48164508214886226 1107 4 0.3167471162501201 1107 5 0.4679410409829927 1107 6 0.6318410609796175 1107 7 0.4542466700334131 1107 8 0.34373912382385896 1107 9 0.8226150839120692 1107 10-11 0.5229772014429059 1107 12-13 0.7147045216467731 1107 14-15 0.6117464458882651 1107 16-17 0.6817102937013217 1107 18-19 0.6965752626479187 1107 20-21 0.6795987845749636 1107 22-23 0.990599850777663 1107 24-25 0.6841146857983773 1107 26-27 1.0091409778050604 1107 28-29 0.9965306001986605 1107 30-31 1.2770321708957582 1107 32-33 1.0806867822476605 1107 34-35 1.0960678427477788 1107 36-37 1.4370451283574326 1107 38-39 1.207956126365339 1107 40-41 1.4472019789762456 1107 42-43 1.1267818872674908 1107 44-45 0.8891439949486895 1107 46-47 0.9827501151228688 1107 48-49 0.8430734242714237 1107 50-51 0.6093623017649605 1107 52-53 0.4874576194564426 1107 54-55 0.6444340392827783 1107 56-57 0.58209538982004 1107 58-59 0.814371190865792 1107 60-61 1.1185196308973246 1107 62-63 1.5732699947286335 1107 64-65 1.4255525038605512 1107 66-67 1.0213718100757987 1107 68-69 1.1120593065407718 1107 70-71 0.3650009803475456 1107 72-73 0.3641183492305835 1107 74-75 0.881152162565563 1107 76-77 0.8799080562012378 1107 78-79 0.7435637630100089 1107 80-81 0.37441964674065176 1107 82-83 0.990988329770957 1107 84-85 0.8918493192715502 1107 86-87 1.0998531084251333 1107 88-89 0.5188179942307052 1107 90-91 1.2002722541516526 1107 92-93 1.5122913472074444 1107 94-95 1.4240440183683098 1107 96-97 0.5679927411831933 1107 98-99 0.42123778244969756 1107 100-101 0.8486261270049162 1108 1 -0.33803692363711235 1108 2 -0.3374436264065679 1108 3 -0.20235980185601932 1108 4 -0.3181735186705126 1108 5 -0.2785791055371547 1108 6 -0.5759733468347932 1108 7 -0.11669350090676289 1108 8 -0.4860533059685679 1108 9 -0.7568720955751118 1108 10-11 -0.3008323223666238 1108 12-13 -0.8205824136401603 1108 14-15 -0.3536992195574058 1108 16-17 -0.6500357380447142 1108 18-19 -0.6401768741042133 1108 20-21 -0.6807186757424972 1108 22-23 -1.3858348256570139 1108 24-25 -0.9250428233591279 1108 26-27 -0.5384780698139835 1108 28-29 -0.9192203155522591 1108 30-31 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62-63 1.0568626397074183 2311 64-65 0.6528509479906788 2311 66-67 0.39940104157037126 2311 68-69 -0.2997747340061423 2311 70-71 0.286830306137265 2311 72-73 0.3338967556426553 2311 74-75 0.6606901163609429 2311 76-77 0.9966454442257557 2311 78-79 0.41687823731458096 2311 80-81 0.7702235128415467 2311 82-83 0.502115156739368 2311 84-85 0.7329148543964905 2311 86-87 1.0100841315274423 2311 88-89 1.5068896585399933 2311 90-91 1.6832991282676382 2311 92-93 1.2954125164672234 2311 94-95 1.3773679221872612 2311 96-97 1.9049092899809281 2311 98-99 1.8563155759433307 2311 100-101 2.5255238167738945 2312 1 0.2520485464483535 2312 2 0.29862474966180486 2312 3 -0.107402536898757 2312 4 -0.09039574089273117 2312 5 0.07936961241156126 2312 6 -0.7143494152108616 2312 7 0.347580003366744 2312 8 0.2699296000143363 2312 9 -0.35405158275460025 2312 10-11 -0.5041656556999499 2312 12-13 -0.2993430974008433 2312 14-15 -0.4630154588736488 2312 16-17 -0.22948018248915503 2312 18-19 -0.9050914040187479 2312 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0.5638686456429127 2313 42-43 -0.12131335082774797 2313 44-45 -0.9722845764798862 2313 46-47 -0.8024879801152309 2313 48-49 -1.0121646709666692 2313 50-51 -0.7763519839493256 2313 52-53 -0.7230185710197503 2313 54-55 -1.2474707226219763 2313 56-57 -0.8940950863704415 2313 58-59 -0.8218192853246862 2313 60-61 -0.9390994167217315 2313 62-63 -1.0191109576523232 2313 64-65 -1.138733210425162 2313 66-67 -0.773866285162299 2313 68-69 -0.8541311696497118 2313 70-71 -0.8911894958429301 2313 72-73 -0.6411197460075115 2313 74-75 -0.7021811707677692 2313 76-77 -0.3343776580844775 2313 78-79 -0.5792933798471331 2313 80-81 -0.6746279723069684 2313 82-83 -0.5432973845147551 2313 84-85 -0.2800554426332127 2313 86-87 -0.6434802249082026 2313 88-89 -0.29499152957881947 2313 90-91 -0.5511563172769165 2313 92-93 -0.1848515099354131 2313 94-95 -7.178863935948243E-4 2313 96-97 -0.20581678262633218 2313 98-99 0.32219016340207673 2313 100-101 1.0619594603382474 2314 1 -0.6349969080970972 2314 2 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2315 18-19 -0.67521960914695 2315 20-21 -0.65336824839207 2315 22-23 -0.19951003933223177 2315 24-25 -0.4558120541283657 2315 26-27 -0.5436062749421993 2315 28-29 -0.9448613411932811 2315 30-31 -1.207216913353328 2315 32-33 -1.2735256719647907 2315 34-35 -1.0266427799628417 2315 36-37 -1.191892600580303 2315 38-39 -0.9678680494588363 2315 40-41 -1.3553621235878595 2315 42-43 -1.14977488928929 2315 44-45 -0.7335666277619382 2315 46-47 -1.112487980115226 2315 48-49 -2.280369799171794 2315 50-51 -1.8455827531800963 2315 52-53 -2.0312236992248742 2315 54-55 -1.6636245687758233 2315 56-57 -1.3658899581653117 2315 58-59 -0.8618192853246924 2315 60-61 -1.2060224936448023 2315 62-63 -1.9150083935497584 2315 64-65 -1.8323229540149057 2315 66-67 -1.050789362085375 2315 68-69 -0.9718234773420171 2315 70-71 -0.8393946240480616 2315 72-73 -0.7465043613921267 2315 74-75 -1.1239760425626422 2315 76-77 -0.2651468888537103 2315 78-79 -0.5198062003599553 2315 80-81 -1.4728331005120943 2315 82-83 -1.5027845640019386 2315 84-85 -0.7597990323767974 2315 86-87 -1.1180956095235857 2315 88-89 -1.2998633244506124 2315 90-91 -1.5326947788153795 2315 92-93 -1.5756207407046432 2315 94-95 -1.6191794248551332 2315 96-97 -1.3712013980109496 2315 98-99 -1.1183226571107454 2315 100-101 -0.8770148986361086 2316 1 -1.295337345667825 2316 2 -1.442703051168067 2316 3 -1.1822158149070532 2316 4 -2.7188189774072598 2316 5 -2.585526653148605 2316 6 -1.9804490002731043 2316 7 -1.5377311999527592 2316 8 -1.5095309808985249 2316 9 -1.7668731595180844 2316 10-11 -1.2586469835007819 2316 12-13 -1.9856708982307154 2316 14-15 -1.796334960948336 2316 16-17 -1.862115037260942 2316 18-19 -1.6804648480021527 2316 20-21 -2.2123000496475242 2316 22-23 -1.8800186040731575 2316 24-25 -2.910945578971429 2316 26-27 -2.2777588443644703 2316 28-29 -1.8317642031998744 2316 30-31 -1.488310646729218 2316 32-33 -1.164897094456542 2316 34-35 -1.8301325129131598 2316 36-37 -1.5317159860468408 2316 38-39 -1.8530792421389108 2316 40-41 -1.68474822849263 2316 42-43 -1.851562313483349 2316 44-45 -1.283529389757895 2316 46-47 -1.6347563065329282 2316 48-49 -1.3731881841063682 2316 50-51 -1.1994501858857092 2316 52-53 -1.066918985957507 2316 54-55 -1.2606242495929365 2316 56-57 -0.4088945331200904 2316 58-59 -0.7845025495017239 2316 60-61 -1.1322806062099744 2316 62-63 -1.124477486006402 2316 64-65 -0.8526336253629196 2316 66-67 -0.9398413888967383 2316 68-69 -0.46371623189037336 2316 70-71 -0.788782856838786 2316 72-73 -1.2156287363256304 2316 74-75 -1.0438824155810487 2316 76-77 -1.318748335816153 2316 78-79 -1.5475644725165658 2316 80-81 -0.9437980967882957 2316 82-83 -1.111540814666899 2316 84-85 -0.777109384541923 2316 86-87 -1.4426088556135959 2316 88-89 -1.5809251395373245 2316 90-91 -1.5744758193516084 2316 92-93 -1.9077837367680566 2316 94-95 -2.1079654659233356 2316 96-97 -2.0833409873289632 2316 98-99 -2.4233976650903166 2316 100-101 -2.5184554774210888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 617.0 3 162.0 4 158.0 5 174.0 6 212.0 7 196.0 8 253.0 9 288.0 10 401.0 11 515.0 12 727.0 13 976.0 14 1206.0 15 1536.0 16 2241.0 17 3050.0 18 2704.0 19 1622.0 20 1453.0 21 1373.0 22 1448.0 23 1643.0 24 1767.0 25 1905.0 26 2210.0 27 2397.0 28 2835.0 29 3369.0 30 4001.0 31 4920.0 32 6305.0 33 8618.0 34 11977.0 35 17499.0 36 26740.0 37 40083.0 38 39993.0 39 8718.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28522920203735 19.362114964831434 15.442638855202523 20.91001697792869 2 14.573678770556636 20.9761399865099 42.079416916977635 22.370764325955832 3 18.363257959821276 27.48812636746891 31.22559174109417 22.92302393161564 4 11.689976067612635 17.22112457948417 39.71514148264295 31.373757870260242 5 12.393529505976908 38.02361642958023 34.911159321112585 14.671694743330278 6 27.91563335646654 38.08049385352312 19.08198376145943 14.921889028550908 7 25.513274765810806 35.05994272678736 22.040964908023103 17.38581759937873 8 24.866181701712584 33.827033480052215 21.233797430882788 20.072987387352413 9 26.567619121579973 16.983046076037837 21.564925326046332 34.884409476335854 10-11 23.133294346316333 27.307493514970773 28.400181508021056 21.15903063069184 12-13 25.507807571743285 25.415368639667708 28.71526867945769 20.361555109131316 14-15 22.644526236361386 25.83651375568048 28.732012315695233 22.7869476922629 16-17 19.8263059810145 29.487459476248713 29.077368819521286 21.608865723215498 18-19 19.644104803493452 27.49344978165939 33.137311984473556 19.725133430373603 20-21 20.80541959144469 26.373453121892297 32.82607535618824 19.995051930474773 22-23 20.24909953066447 26.246892319633265 32.79042409983386 20.713584049868416 24-25 19.938009012461254 26.543590141589746 33.27067942704417 20.247721418904828 26-27 20.130857485140304 26.399212333002716 33.15452387130569 20.315406310551296 28-29 19.69924447301083 26.32338495494731 33.83184370414388 20.145526867897985 30-31 19.772830333261393 26.9836727864416 33.32953423913439 19.91396264116262 32-33 19.35161586792732 27.11583276549 32.98422082598774 20.54833054059494 34-35 19.752816998741533 26.66710312870538 32.87723050287701 20.70284936967607 36-37 20.138855208404063 26.71471389910081 32.79369113324862 20.352739759246504 38-39 20.108824798863257 26.685596232838517 33.08947978448422 20.11609918381401 40-41 19.641956386776346 27.06546833773087 33.115154043147605 20.17742123234518 42-43 19.569603554141487 27.435712137819984 32.58916879262011 20.405515515418415 44-45 19.600697349055206 27.592812164328024 32.1389169752121 20.66757351140466 46-47 19.886152883876427 27.562105589806123 31.232802321550224 21.318939204767226 48-49 20.06589953593925 27.707384724303303 31.08309936331059 21.143616376446854 50-51 20.63847994356514 27.955860570990833 30.73154412852021 20.674115356923817 52-53 20.047664997017907 28.125257599487952 30.888421236380566 20.938656167113574 54-55 19.915763847802683 27.989243765942796 30.929749861788697 21.165242524465825 56-57 20.090973234180915 28.609045435699237 30.129549570956453 21.170431759163396 58-59 20.03428768189567 27.8338866081626 30.7265157265036 21.40530998343813 60-61 20.085546767020848 27.421763989854657 31.08271945606999 21.409969787054507 62-63 20.542789799897186 27.453514651250764 30.861955246030437 21.141740302821617 64-65 20.427138236848613 27.30576580857574 31.266819568158343 21.000276386417305 66-67 19.87897896077124 27.636694355180985 31.096828957739646 21.38749772630813 68-69 19.93215637494878 27.988041288107485 31.04100441541248 21.03879792153126 70-71 19.78517196908726 27.813224214333506 30.710323963191733 21.691279853387506 72-73 19.73941920783439 27.62107916808067 30.217191060260824 22.422310563824112 74-75 19.84435986326941 27.71485369342287 30.602681277121867 21.838105166185848 76-77 20.190967128728236 27.300588952711628 30.79623787967325 21.712206038886883 78-79 19.697770750067907 27.032080633269956 30.98638003957937 22.283768577082768 80-81 19.539965134914304 27.858852167672453 30.659454426871818 21.94172827054143 82-83 19.74588561699981 27.876187750706947 30.40447792323699 21.97344870905625 84-85 19.65314694811998 27.25810989096271 29.976766520507546 23.11197664040976 86-87 19.879802970001673 27.120970695304408 30.212964108235536 22.786262226458383 88-89 20.111346958578448 26.358905937291528 30.552246952513794 22.97750015161623 90-91 19.705373751876582 26.37981756843609 31.391713697404196 22.523094982283133 92-93 19.302807301798282 26.848128222915385 30.76656269252599 23.082501782760342 94-95 18.818007616367915 27.104082276178232 30.82906832900769 23.248841778446163 96-97 18.835548309776463 27.190109511540683 30.71150962896112 23.262832549721733 98-99 19.055685665400624 27.97776941838877 29.798929728549066 23.167615187661543 100-101 19.305308832522787 28.10604075055573 29.130871603715892 23.457778813205586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 137.5 2 115.5 3 128.5 4 122.0 5 125.5 6 202.5 7 437.0 8 764.5 9 896.0 10 732.5 11 552.5 12 537.0 13 561.5 14 550.5 15 589.0 16 631.5 17 645.5 18 646.0 19 691.0 20 762.5 21 822.5 22 932.5 23 1085.0 24 1260.5 25 1469.0 26 1774.0 27 2163.5 28 2501.0 29 2785.0 30 3184.5 31 3636.0 32 4180.5 33 4683.5 34 5217.5 35 5665.0 36 6194.5 37 6688.0 38 6763.5 39 6637.0 40 6531.0 41 6652.5 42 6577.0 43 6591.0 44 6798.5 45 6884.5 46 6993.0 47 7335.5 48 9201.5 49 9039.5 50 6872.0 51 6077.5 52 5561.5 53 5327.0 54 5001.0 55 4509.5 56 4009.5 57 3517.5 58 3158.0 59 2740.5 60 2225.0 61 1855.0 62 1456.0 63 1055.0 64 762.0 65 546.5 66 403.0 67 305.5 68 222.0 69 161.0 70 109.5 71 74.0 72 53.5 73 38.5 74 26.5 75 19.0 76 16.5 77 9.0 78 5.0 79 4.5 80 3.0 81 3.5 82 2.0 83 2.0 84 2.0 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07174087969830052 2 0.1071265838738136 3 0.08192032884468099 4 0.14590543776478687 5 0.12360759677747724 6 0.1187602400411056 7 0.1299091605347604 8 0.11391288330473394 9 0.13039389620839756 10-11 0.11948734355156133 12-13 0.10470290550562777 14-15 0.10639948036335788 16-17 0.11924497571474275 18-19 0.0959776633801588 20-21 0.076103500761035 22-23 0.07537639725057925 24-25 0.06737825863556603 26-27 0.057925912999641296 28-29 0.07295271888239344 30-31 0.052593820589632474 32-33 0.052593820589632474 34-35 0.04580752115871215 36-37 0.054290395447362555 38-39 0.04604988899553074 40-41 0.05913775218373421 42-43 0.056229338141911216 44-45 0.042899107116889164 46-47 0.02738756556049986 48-49 0.03635517552278743 50-51 0.02060126612957954 52-53 0.0324772901336901 54-55 0.04459568197461924 56-57 0.04047542874870333 58-59 0.04920067087417231 60-61 0.04604988899553074 62-63 0.05041251005826523 64-65 0.031507818786415766 66-67 0.06568168377783594 68-69 0.042899107116889164 70-71 0.019389426945486624 72-73 0.013572598861840639 74-75 0.025690990702769777 76-77 0.040960164422340496 78-79 0.06301563757283153 80-81 0.03611280768596884 82-83 0.06350037324646869 84-85 0.06301563757283153 86-87 0.06616641945147311 88-89 0.08991846745969423 90-91 0.06713589079874745 92-93 0.076103500761035 94-95 0.079011914802858 96-97 0.09743187040107029 98-99 0.13354467808703915 100-101 0.12797021784021173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 206298.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.68059105399101 #Duplication Level Percentage of deduplicated Percentage of total 1 84.91289245940308 57.46954749756378 2 9.698246621264062 13.127661270290462 3 2.2369900610264235 4.542024285365153 4 0.989212279518915 2.6780188702282373 5 0.5697835087301055 1.9281642321835195 6 0.33952848932168606 1.3787693302176232 7 0.2609717363134417 1.2363904953456017 8 0.18301223605112538 0.9909101044842149 9 0.12590061350544632 0.7668925142497822 >10 0.636259756967334 7.323884779377804 >50 0.023601118949201763 1.1880198995130324 >100 0.017700839211901326 2.7400996785967253 >500 0.0029501398686502204 1.3071546681476727 >1k 0.0029501398686502204 3.3224623744364474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2311 1.12022414177549 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1590 0.7707297210830933 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.5288466199381477 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 716 0.34707074232421065 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 652 0.316047659211432 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 596 0.28890246148775073 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 428 0.20746686831670688 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 380 0.18419955598212295 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 368 0.17838272789847695 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 364 0.17644378520392828 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 335 0.1623864506684505 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 307 0.14881385180660986 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 286 0.1386344026602294 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 269 0.13039389620839756 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 2.4236783681858283E-4 18-19 0.0 0.0 0.0 0.0 4.8473567363716566E-4 20-21 0.0 0.0 0.0 0.0 4.8473567363716566E-4 22-23 0.0 0.0 0.0 4.8473567363716566E-4 4.8473567363716566E-4 24-25 0.0 0.0 0.0 0.0019389426945486629 4.8473567363716566E-4 26-27 0.0 0.0 0.0 0.0033931497154601597 4.8473567363716566E-4 28-29 0.0 0.0 0.0 0.008240506451831817 4.8473567363716566E-4 30-31 0.0 0.0 0.0 0.01720811641411938 4.8473567363716566E-4 32-33 0.0 0.0 0.0 0.023509680171402536 4.8473567363716566E-4 34-35 0.0 0.0 0.0 0.029568876091867105 4.8473567363716566E-4 36-37 0.0 0.0 0.0 0.04265673928007058 4.8473567363716566E-4 38-39 0.0 0.0 0.0 0.054775131120999715 4.8473567363716566E-4 40-41 0.0 0.0 0.0 0.07004430484057045 4.8473567363716566E-4 42-43 0.0 0.0 0.0 0.10906552656836227 4.8473567363716566E-4 44-45 0.0 0.0 0.0 0.15341884070616293 4.8473567363716566E-4 46-47 0.0 0.0 0.0 0.1890469127184946 4.8473567363716566E-4 48-49 0.0 0.0 0.0 0.24115599763448992 4.8473567363716566E-4 50-51 0.0 0.0 0.0 0.2976277036132197 4.8473567363716566E-4 52-53 0.0 0.0 0.0 0.38730380323609537 4.8473567363716566E-4 54-55 0.0 0.0 0.0 0.46049888995530736 4.8473567363716566E-4 56-57 0.0 0.0 0.0 0.6238548119710322 4.8473567363716566E-4 58-59 0.0 0.0 0.0 0.8613752920532434 4.8473567363716566E-4 60-61 0.0 0.0 0.0 1.0976839329513617 4.8473567363716566E-4 62-63 0.0 0.0 0.0 1.4488749285014881 4.8473567363716566E-4 64-65 0.0 0.0 0.0 1.6357405306886155 4.8473567363716566E-4 66-67 0.0 0.0 0.0 1.9925059864855692 4.8473567363716566E-4 68-69 0.0 0.0 0.0 2.3941094920939614 4.8473567363716566E-4 70-71 0.0 0.0 0.0 2.7040979553849285 4.8473567363716566E-4 72-73 0.0 0.0 0.0 3.3228630427827706 4.8473567363716566E-4 74-75 0.0 0.0 0.0 3.8626162153777543 4.8473567363716566E-4 76-77 0.0 0.0 0.0 4.1890856915723855 4.8473567363716566E-4 78-79 0.0 0.0 0.0 4.501982568905175 4.8473567363716566E-4 80-81 0.0 0.0 0.0 5.115657931729828 4.8473567363716566E-4 82-83 0.0 0.0 0.0 5.480663893978614 4.8473567363716566E-4 84-85 0.0 0.0 0.0 6.140146777961977 4.8473567363716566E-4 86-87 0.0 0.0 0.0 7.479471444221466 4.8473567363716566E-4 88-89 0.0 0.0 0.0 9.070616292935462 4.8473567363716566E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTA 65 2.5602785E-6 43.892506 5 GTGTAGC 95 3.452075E-5 30.031717 1 TATCCAG 80 4.4533127E-4 29.71889 5 GTATCAA 2390 0.0 29.445322 1 ATGGGAG 300 0.0 28.53013 5 TAGGCTG 70 0.008332774 27.171553 5 CGATTCT 35 0.008386942 27.141851 72-73 GAGTACT 1250 0.0 26.61518 12-13 GTACATG 1420 0.0 26.453995 1 GTCCTAA 90 8.8676024E-4 26.416788 1 TATCAAC 2695 0.0 25.936483 2 ATCAACG 2740 0.0 25.516726 3 TACATGG 1400 0.0 25.473331 2 TCAACGC 2755 0.0 25.205156 4 AACGCAG 2795 0.0 25.008524 6 CAACGCA 2805 0.0 24.919367 5 ACATGGG 1345 0.0 24.753345 3 GTACTTT 1345 0.0 24.55563 14-15 ACGCAGA 2955 0.0 23.654425 7 CGCAGAG 2965 0.0 23.41997 8 >>END_MODULE