FastQCFastQC Report
Thu 26 May 2016
SRR936927_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936927_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206298
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23301.129434119574596No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19450.9428108852242872No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.6296716400546781No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA8210.397967988056113No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT6970.33786076452510444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6460.313139245169609No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5820.28211616205683043No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.23558153738766252No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG4120.19971109753851224No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.17983693491938846No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3660.17741325655120263No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.17208116414119382No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3550.17208116414119382No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2530.1226381254302029No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA2370.11488235465200826No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2100.10179449146380479No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2070.10034028444289328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAGC405.3808856E-447.494557
GTGTAGC554.9382896E-543.1978031
CTAGTAG459.6006494E-442.227613
CGTAGCT459.6121174E-442.2173738
TCTACCC550.002573128634.5498623
TATTCGT550.002573128634.5498624
ATATTCG550.002573128634.5498623
CTAGGAC1351.1856173E-831.6707083
GATAGCT600.003941040531.6707064
CTGCCGC300.003950262431.66302984-85
TAAGGTA650.00582946629.23454
TACCTGG1153.8405615E-628.9167332
CAATAAG1205.3382428E-627.7185881
TGCGCGC350.00837031327.15290626-27
TTCGTAG700.00838058527.139746
ATGTAAG950.001220505825.0092541
GCTTATA1151.273569E-424.7917821
AATAAGG1401.768563E-523.7530332
TTATACT1503.0146972E-522.1694953
GTACATG13750.022.1172751
Produced by FastQC (version 0.11.5)