##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936927_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 206298 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25947415874124 33.0 31.0 34.0 31.0 34.0 2 32.26774374933349 33.0 31.0 34.0 30.0 34.0 3 32.42793434740036 34.0 31.0 34.0 31.0 34.0 4 36.14351084353702 37.0 35.0 37.0 35.0 37.0 5 36.097601527886845 37.0 35.0 37.0 35.0 37.0 6 36.16851350958322 37.0 36.0 37.0 35.0 37.0 7 36.10982656157597 37.0 35.0 37.0 35.0 37.0 8 36.11466422359887 37.0 35.0 37.0 35.0 37.0 9 37.826008977304674 39.0 38.0 39.0 35.0 39.0 10-11 37.74964614295824 39.0 37.0 39.0 35.0 39.0 12-13 37.6526771951255 39.0 37.0 39.0 35.0 39.0 14-15 38.827029345897685 40.0 38.0 41.0 35.0 41.0 16-17 38.76371801956393 40.0 38.0 41.0 35.0 41.0 18-19 38.881780240235 40.0 38.0 41.0 35.0 41.0 20-21 38.96396717370018 40.0 38.0 41.0 35.0 41.0 22-23 38.90882121978885 40.0 38.0 41.0 35.0 41.0 24-25 38.7851506073738 40.0 38.0 41.0 35.0 41.0 26-27 38.603760094620405 40.0 38.0 41.0 34.5 41.0 28-29 38.4058085875772 40.0 38.0 41.0 34.0 41.0 30-31 38.18790536020708 40.0 37.5 41.0 34.0 41.0 32-33 38.07461051488623 40.0 37.0 41.0 34.0 41.0 34-35 37.94851137674626 40.0 37.0 41.0 34.0 41.0 36-37 37.728846135202474 40.0 37.0 41.0 33.0 41.0 38-39 37.522128183501536 40.0 36.0 41.0 33.0 41.0 40-41 37.2848840027533 40.0 36.0 41.0 33.0 41.0 42-43 36.97768034590737 40.0 36.0 41.0 32.5 41.0 44-45 36.42641470106351 39.5 35.0 41.0 30.5 41.0 46-47 35.98329843236483 39.0 35.0 41.0 29.5 41.0 48-49 35.47215678290628 39.0 35.0 41.0 27.0 41.0 50-51 35.130161223085054 39.0 35.0 41.0 25.5 41.0 52-53 34.83554615168349 39.0 35.0 40.0 24.0 41.0 54-55 34.57915491182658 38.5 35.0 40.0 23.0 41.0 56-57 34.28739250986437 38.0 34.0 40.0 22.5 41.0 58-59 33.97161872630855 38.0 34.0 40.0 20.5 41.0 60-61 33.620151431424446 38.0 34.0 40.0 19.0 41.0 62-63 33.227057460566755 37.0 33.0 40.0 16.5 41.0 64-65 32.79872805359237 36.5 33.0 39.5 12.0 41.0 66-67 32.41766764583272 36.0 32.0 39.0 8.0 40.0 68-69 31.982079322145637 35.5 32.0 39.0 6.5 40.0 70-71 31.493257326779705 35.0 31.0 38.0 2.0 40.0 72-73 31.15911690854977 35.0 31.0 37.5 2.0 40.0 74-75 30.71347516699144 35.0 31.0 37.0 2.0 39.0 76-77 29.33340362000601 33.5 29.0 35.5 2.0 38.5 78-79 29.97381700258849 34.0 30.0 36.0 2.0 38.5 80-81 29.790007658823644 34.0 30.0 36.0 2.0 37.5 82-83 29.454335475865008 34.0 30.0 35.0 2.0 37.0 84-85 29.07943848219566 34.0 29.5 35.0 2.0 37.0 86-87 28.719127184946046 34.0 29.0 35.0 2.0 36.0 88-89 28.341011546403745 34.0 29.0 35.0 2.0 36.0 90-91 27.935842809915755 34.0 28.5 35.0 2.0 35.5 92-93 27.527874724912508 34.0 27.0 35.0 2.0 35.0 94-95 27.090870973058394 33.5 26.5 35.0 2.0 35.0 96-97 26.432735654247736 33.0 25.0 35.0 2.0 35.0 98-99 25.838701780918864 33.0 24.5 35.0 2.0 35.0 100-101 23.16666181930993 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34645692704521736 1101 2 0.16562502773532373 1101 3 0.18813749475896913 1101 4 0.21653549599975008 1101 5 0.28246303211531654 1101 6 0.21157398167276398 1101 7 0.2694940833656787 1101 8 0.25454629470906553 1101 9 0.414020958936554 1101 10-11 0.32341329645232975 1101 12-13 0.36304388777623586 1101 14-15 0.5213509879440821 1101 16-17 0.40751598672084555 1101 18-19 0.3867410892974945 1101 20-21 0.5004722811625584 1101 22-23 0.2806010840599029 1101 24-25 0.35628589843552305 1101 26-27 0.11235768175055227 1101 28-29 0.2836656960434638 1101 30-31 0.13789801467849827 1101 32-33 0.3225560281537625 1101 34-35 0.22566714121460762 1101 36-37 0.35124565626318116 1101 38-39 0.10284313817315649 1101 40-41 0.1772790348005273 1101 42-43 0.3223437880646074 1101 44-45 0.48970749542781533 1101 46-47 0.18572585853463153 1101 48-49 0.04651421056995275 1101 50-51 -0.053781318422906566 1101 52-53 -0.1241889633399893 1101 54-55 0.13975723585873112 1101 56-57 0.19160112078299818 1101 58-59 0.37857109435910985 1101 60-61 0.25116473385327964 1101 62-63 0.27349047139576044 1101 64-65 0.5539499594280457 1101 66-67 0.45767112056809367 1101 68-69 0.3526275443061522 1101 70-71 0.09053229524793949 1101 72-73 0.4564074356483445 1101 74-75 0.5307933735760173 1101 76-77 0.20217041913729972 1101 78-79 0.5274612989737903 1101 80-81 0.707751195299462 1101 82-83 0.7384757572570102 1101 84-85 0.8128164955474624 1101 86-87 0.5090566360794853 1101 88-89 0.8491918292668217 1101 90-91 0.905131508196515 1101 92-93 1.0864717448545242 1101 94-95 0.8894957394110179 1101 96-97 0.7304696046008878 1101 98-99 0.6066069628540802 1101 100-101 -0.025321403178079294 1102 1 0.396948715219267 1102 2 0.2514702814611667 1102 3 0.3496789685110713 1102 4 0.27520455826221024 1102 5 0.3212670769866648 1102 6 0.286275108063478 1102 7 0.32309553915080613 1102 8 0.28680852030969106 1102 9 0.38656374565942286 1102 10-11 0.48486493536233866 1102 12-13 0.4123194503957279 1102 14-15 0.5364870071088532 1102 16-17 0.6720358310071148 1102 18-19 0.5822447402776234 1102 20-21 0.5678115574162206 1102 22-23 0.49628993302219016 1102 24-25 0.707162364010351 1102 26-27 0.6436985243553082 1102 28-29 0.566256164336373 1102 30-31 0.4530616753331387 1102 32-33 0.4114625768834088 1102 34-35 0.3698797042717601 1102 36-37 0.6861983202117656 1102 38-39 0.7963289603923087 1102 40-41 0.7796861478772499 1102 42-43 0.9814459003351246 1102 44-45 0.8991862229978906 1102 46-47 0.550713304691314 1102 48-49 0.4134365610179742 1102 50-51 0.731294580501384 1102 52-53 0.8018032509736912 1102 54-55 0.5096648672133952 1102 56-57 0.5307552911927829 1102 58-59 0.7092278100894163 1102 60-61 0.8564143134722926 1102 62-63 0.8766853669339554 1102 64-65 1.0483180058657524 1102 66-67 0.4824609300721576 1102 68-69 0.4623251003377504 1102 70-71 0.6724824023587139 1102 72-73 0.598747287766372 1102 74-75 0.5993696065016394 1102 76-77 0.7150582637921268 1102 78-79 0.6851444814306582 1102 80-81 1.0057517941984067 1102 82-83 1.168291711003583 1102 84-85 0.9960772699698701 1102 86-87 0.9885650782511881 1102 88-89 0.8557820211448295 1102 90-91 1.1288225684779967 1102 92-93 0.6326698882677881 1102 94-95 1.057675777648413 1102 96-97 0.9939805345066759 1102 98-99 0.8019585856398805 1102 100-101 0.9439550808244057 1103 1 -0.07203065051955093 1103 2 -0.23524900949576377 1103 3 -0.05573579398720341 1103 4 0.04950867126594716 1103 5 0.08629962095564991 1103 6 0.15395271504311836 1103 7 0.12561596568453126 1103 8 0.24215404032077004 1103 9 0.21058396446568395 1103 10-11 0.12316641715155896 1103 12-13 0.2992083854462777 1103 14-15 0.34721959262331836 1103 16-17 0.15968297129239062 1103 18-19 0.23507329587933867 1103 20-21 0.20410846920129444 1103 22-23 0.30451940262474153 1103 24-25 0.25511971063824035 1103 26-27 0.4556255427738023 1103 28-29 0.31106990204582985 1103 30-31 0.568623924329124 1103 32-33 0.5997640586726334 1103 34-35 0.5541131899385618 1103 36-37 0.5404463468008842 1103 38-39 0.8131583469038759 1103 40-41 0.4083767724444911 1103 42-43 0.6022982753524388 1103 44-45 1.0309858352399672 1103 46-47 0.7715933767034997 1103 48-49 0.404621992398674 1103 50-51 0.4393163226423553 1103 52-53 0.449521711020779 1103 54-55 0.476068412428738 1103 56-57 0.5088712031163425 1103 58-59 0.30546101878626786 1103 60-61 0.45861276244058047 1103 62-63 0.5856418826520127 1103 64-65 0.4388975654835292 1103 66-67 0.6786733660228563 1103 68-69 0.9932280223707153 1103 70-71 0.7903988959191608 1103 72-73 0.6683463737655408 1103 74-75 0.4678331157108886 1103 76-77 0.7089822355411926 1103 78-79 0.9804636892966059 1103 80-81 0.7751812361039612 1103 82-83 0.6472662297682987 1103 84-85 0.6040193832505771 1103 86-87 0.41490145825395786 1103 88-89 0.7619480646291095 1103 90-91 1.0284625075446066 1103 92-93 0.9147880159509363 1103 94-95 1.1217735480403235 1103 96-97 1.29164013843965 1103 98-99 1.7675312210089444 1103 100-101 2.2578624343765448 1104 1 0.08215944318861546 1104 2 0.13422890215082361 1104 3 -0.03852026520540619 1104 4 -0.02855023100982379 1104 5 -0.08149154370298817 1104 6 -0.08414634787521891 1104 7 -0.003647755868350089 1104 8 0.09922231341313648 1104 9 -0.1210224612371249 1104 10-11 0.056359188235902025 1104 12-13 -0.07909817412534181 1104 14-15 0.20767474657245089 1104 16-17 0.28169100342091014 1104 18-19 -0.054920010680234554 1104 20-21 0.1274284156537675 1104 22-23 -0.08738822789734257 1104 24-25 0.2614918659260539 1104 26-27 0.12829622550473374 1104 28-29 0.12303777353177026 1104 30-31 0.03750611977758922 1104 32-33 0.28962349642363705 1104 34-35 0.15064598779665772 1104 36-37 0.3259684351542944 1104 38-39 0.23809141249958543 1104 40-41 0.24282791032936046 1104 42-43 0.5238980076148181 1104 44-45 0.08087204675268111 1104 46-47 0.4492573659939936 1104 48-49 0.6688388598685506 1104 50-51 0.6869267644094279 1104 52-53 0.6578952049966844 1104 54-55 0.5790135262172171 1104 56-57 0.4544334521123261 1104 58-59 0.3184998407407562 1104 60-61 0.3498242751581202 1104 62-63 0.5831987768956424 1104 64-65 0.20004214379678587 1104 66-67 0.449663995206258 1104 68-69 0.5069228014871783 1104 70-71 0.27363182764607075 1104 72-73 0.06970514217250212 1104 74-75 0.16611290152079405 1104 76-77 0.12210807222124842 1104 78-79 0.5344931404344919 1104 80-81 0.923146430213734 1104 82-83 0.8397093355246561 1104 84-85 0.7515561971729348 1104 86-87 0.7216685265270506 1104 88-89 0.9865866188459798 1104 90-91 0.7529604995124792 1104 92-93 0.8349687388425018 1104 94-95 0.6929248197940048 1104 96-97 0.7475437528974815 1104 98-99 0.966326401731834 1104 100-101 0.810238605728621 1105 1 0.3246991257282943 1105 2 0.27179503971695596 1105 3 0.023278676593534442 1105 4 0.1657354832758955 1105 5 0.14644496423352393 1105 6 0.3461182023893272 1105 7 0.248839016618426 1105 8 0.30684136103218407 1105 9 0.4901944699798051 1105 10-11 0.35191474379145404 1105 12-13 0.3475684925413205 1105 14-15 0.5879922068899077 1105 16-17 0.2542835960135008 1105 18-19 0.6329106771504556 1105 20-21 0.13726968549504193 1105 22-23 0.5848869043777896 1105 24-25 0.31905800349219504 1105 26-27 0.3514708286793322 1105 28-29 0.5252599957539914 1105 30-31 0.629781252052716 1105 32-33 0.5044383112384523 1105 34-35 0.6145613317120038 1105 36-37 0.5856509748368381 1105 38-39 0.7534353278435049 1105 40-41 0.6951030426044937 1105 42-43 0.4142683779851879 1105 44-45 0.8312424171230575 1105 46-47 0.4938076305442607 1105 48-49 0.5924367434664362 1105 50-51 0.45105374853640967 1105 52-53 0.3702761573776314 1105 54-55 0.31287596007965846 1105 56-57 0.34057101824988933 1105 58-59 0.5598755021164195 1105 60-61 0.8745861799200227 1105 62-63 0.17526226895914476 1105 64-65 0.1767617205163603 1105 66-67 0.23453172007398138 1105 68-69 0.05845719302157093 1105 70-71 -0.4099660559518128 1105 72-73 -0.41061231814495613 1105 74-75 -0.13748498207708693 1105 76-77 -0.011542721429545821 1105 78-79 0.02750901345036283 1105 80-81 0.2492839963512985 1105 82-83 -0.09447055865523879 1105 84-85 0.4101805357972701 1105 86-87 0.8890759339344569 1105 88-89 0.5492321214914817 1105 90-91 0.8857647323167122 1105 92-93 0.6286195324932962 1105 94-95 0.7177925446617301 1105 96-97 0.7913532767070066 1105 98-99 0.7758502112556442 1105 100-101 0.3975401930302098 1106 1 0.264634190663358 1106 2 0.09635011427203466 1106 3 0.09158074489560164 1106 4 -0.0586512411108302 1106 5 -0.13043093764238023 1106 6 0.06605567232679732 1106 7 0.25034214312155 1106 8 0.14179807098889796 1106 9 0.12337147815681249 1106 10-11 0.36454100641771703 1106 12-13 0.2610785935514244 1106 14-15 0.39787676677446626 1106 16-17 0.203357670087577 1106 18-19 0.0820749388147135 1106 20-21 0.2657870015123507 1106 22-23 0.4147329842238676 1106 24-25 0.20295651239070622 1106 26-27 0.22178107398957536 1106 28-29 0.09091656141055893 1106 30-31 0.2557131904846557 1106 32-33 0.49454773884787784 1106 34-35 -0.23819239604172537 1106 36-37 -0.5922891406032775 1106 38-39 -0.3841308097226346 1106 40-41 -0.6366670391655873 1106 42-43 -0.26544542672861837 1106 44-45 -0.2259966401160014 1106 46-47 0.11688362862025059 1106 48-49 0.5667934053230965 1106 50-51 -0.2527702052875469 1106 52-53 -0.7127866131851377 1106 54-55 -0.3943450596413598 1106 56-57 -0.37708169940282943 1106 58-59 -0.05920217946125916 1106 60-61 -0.24545350261966092 1106 62-63 -0.38813960694273675 1106 64-65 -0.06473058347594218 1106 66-67 -0.24917438863212737 1106 68-69 -0.717546895482517 1106 70-71 -0.2896257481115043 1106 72-73 -0.7596382921709335 1106 74-75 0.18045633586423193 1106 76-77 0.19624948636266026 1106 78-79 -0.45409271815136876 1106 80-81 -1.0030909435236417 1106 82-83 -0.850543189727869 1106 84-85 -0.484630671513937 1106 86-87 -0.9084829886244634 1106 88-89 -0.3852315629722014 1106 90-91 -0.24097889442691312 1106 92-93 -0.21048580661204497 1106 94-95 -0.6428075034383198 1106 96-97 -0.2210926107388822 1106 98-99 0.3242809471863808 1106 100-101 0.35821840370841684 1107 1 0.2770800781092504 1107 2 0.2961336640555814 1107 3 0.29417814749300675 1107 4 0.2577989753393837 1107 5 0.4480322658208209 1107 6 0.26886952514065143 1107 7 0.17793954571895654 1107 8 -0.11220625801543349 1107 9 0.18754896733430115 1107 10-11 0.08207347395018161 1107 12-13 0.11264785762068641 1107 14-15 0.28164300054070424 1107 16-17 0.5997862415161492 1107 18-19 0.46730221154198404 1107 20-21 -0.019873171647816434 1107 22-23 -0.09976918027830095 1107 24-25 0.2322855167197062 1107 26-27 0.5863914635999734 1107 28-29 0.4268472973412969 1107 30-31 0.4578235800950452 1107 32-33 0.36581397261411297 1107 34-35 0.573820590971259 1107 36-37 0.3840636732495355 1107 38-39 0.4253929998011756 1107 40-41 0.8120342595357144 1107 42-43 0.7264376901544978 1107 44-45 0.07833236421300427 1107 46-47 -0.12153628479965306 1107 48-49 -0.043542092512403485 1107 50-51 0.4097839072665721 1107 52-53 0.37979996690144446 1107 54-55 0.27107701828071384 1107 56-57 -0.2493760716971991 1107 58-59 0.43500777724869266 1107 60-61 0.6063322116660501 1107 62-63 0.3054209991178709 1107 64-65 0.08575642951107199 1107 66-67 0.19379097933324374 1107 68-69 0.3621608967252712 1107 70-71 -0.22636817235392925 1107 72-73 -0.058231365764005005 1107 74-75 -0.2697601143522199 1107 76-77 -1.0067808166676429 1107 78-79 -0.6201100341686825 1107 80-81 -0.4676472205799165 1107 82-83 -0.1987033628880397 1107 84-85 -0.4681263425096063 1107 86-87 -0.13737909252056824 1107 88-89 -0.4915086192492595 1107 90-91 -0.7470395004875208 1107 92-93 -0.46503126115749893 1107 94-95 -0.9800910532218694 1107 96-97 -0.19436100900728093 1107 98-99 -0.8860545506491171 1107 100-101 -0.3913486958586816 1108 1 -0.12707132604215587 1108 2 -0.2503864824645632 1108 3 -0.046725393410532945 1108 4 -0.25795194041153025 1108 5 -0.1307223129337558 1108 6 -0.024659168388041053 1108 7 -0.2672374994580906 1108 8 0.07973513392595777 1108 9 -0.29572331593798395 1108 10-11 -0.2976579057811932 1108 12-13 -0.378927233954407 1108 14-15 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-0.09736335481852976 2316 76-77 -0.6496577184657077 2316 78-79 -0.5283099946508045 2316 80-81 -0.4325751004455576 2316 82-83 -0.2364824579285454 2316 84-85 -0.9946033233434299 2316 86-87 -0.8350672964328396 2316 88-89 -0.6920025927722797 2316 90-91 -0.9888099015940206 2316 92-93 -1.0094296014064632 2316 94-95 -0.8844380202244366 2316 96-97 -0.07892927614816259 2316 98-99 -0.42993915843414143 2316 100-101 -1.1431961568347724 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 2.0 5 3.0 6 11.0 7 33.0 8 67.0 9 114.0 10 255.0 11 381.0 12 663.0 13 901.0 14 1094.0 15 1707.0 16 2405.0 17 3682.0 18 3393.0 19 1834.0 20 1385.0 21 1313.0 22 1332.0 23 1452.0 24 1461.0 25 1683.0 26 1929.0 27 2347.0 28 2816.0 29 3419.0 30 4128.0 31 5277.0 32 6706.0 33 9000.0 34 12707.0 35 18688.0 36 29040.0 37 45027.0 38 36580.0 39 3462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.43156914842038 20.19180420068654 15.647363419506236 19.72926323138685 2 15.225695117694963 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21.20706077961361 26.62989076296266 33.1320030344968 19.031045422926933 22-23 20.884109395146826 26.57999592822034 32.80133593151655 19.73455874511629 24-25 20.485662556866245 26.57716721886808 33.37082001303955 19.56635021122613 26-27 20.62221726706238 26.745017438661716 33.22822469818633 19.40454059608957 28-29 20.108631107470146 26.624800952037013 33.91462205730157 19.351945883191263 30-31 20.31138270306171 27.144543530797705 33.30069058798149 19.243383178159096 32-33 20.105187896120892 27.356851149432256 32.890607011718515 19.64735394272834 34-35 20.205822258955035 26.80779177341131 33.224104375056044 19.7622815925776 36-37 20.905944457640345 27.01109586849772 32.70154585952989 19.381413814332042 38-39 20.75857204834812 26.81493294812538 33.0322280418138 19.394266961712688 40-41 20.16016248930513 27.379350963810744 33.094592334596115 19.365894212288012 42-43 20.461133516888356 27.772472563694883 32.330971031915304 19.435422887501456 44-45 20.555993165210438 27.804990365855137 31.862722524509508 19.776293944424918 46-47 20.646104179390978 28.206768848946666 30.695401797399878 20.451725174262474 48-49 20.964817884807417 28.358491114795104 30.414497474527142 20.26219352587034 50-51 21.504813425239217 28.62097548206963 29.969510126128224 19.90470096656293 52-53 20.932695733526586 28.918609075859997 30.081994430292614 20.066700760320806 54-55 20.710499463138326 28.880007174314017 30.110305078008547 20.29918828453911 56-57 20.87281928481752 29.304594831723197 29.604405299156074 20.218180584303212 58-59 20.8351612129203 28.641196729933515 29.77621906440034 20.747422992745843 60-61 20.55066773310066 28.643689861605953 30.094282459584576 20.711359945708814 62-63 21.36908743662081 28.4106001997111 30.0422204771738 20.178091886494297 64-65 21.083386856360352 28.37067826803524 30.373853294392806 20.1720815812116 66-67 20.612657417910015 28.894851137674628 30.03082918884332 20.461662255572037 68-69 20.757108648653887 28.93968918748606 29.94236492840454 20.360837235455506 70-71 20.500925845136646 29.182299392141463 29.610078624126263 20.706696138595625 72-73 20.4781432684757 28.655149347061048 29.45302426586782 21.41368311859543 74-75 20.927301591148705 28.550758007246817 29.319065912092974 21.202874489511505 76-77 20.71425974624026 28.708782219852395 29.519262230516606 21.057695803390732 78-79 20.665978342010103 28.162657902645687 29.646676167485868 21.52468758785834 80-81 20.65471891103172 29.067755117869766 29.10338508970512 21.1741408813934 82-83 20.556534468926703 29.383478473735362 28.808572211086574 21.251414846251354 84-85 20.272235472049328 28.864909933492335 28.60484168072441 22.25801291373393 86-87 20.448243768602094 28.82051828943159 28.608199947647535 22.123037994318786 88-89 20.728124984851938 27.93908781998769 29.016660445862037 22.31612674929834 90-91 19.964565822257125 28.138663486800645 29.64813037450678 22.24864031643545 92-93 20.125789212181434 28.415031689671473 29.034040645184746 22.425138452962347 94-95 19.73043849189037 29.055783381322165 28.750884642604387 22.462893484183073 96-97 19.825636774789317 29.354051387949692 28.34117410388597 22.47913773337502 98-99 19.875226920737102 29.688140013427404 27.604225997299974 22.832407068535517 100-101 19.85835886299298 30.361955636561056 26.99034397176872 22.78934152867724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 390.0 1 273.0 2 157.5 3 154.0 4 142.0 5 155.5 6 240.5 7 480.0 8 735.5 9 829.0 10 760.5 11 655.5 12 655.5 13 671.5 14 682.5 15 682.0 16 691.0 17 708.5 18 734.5 19 780.5 20 827.0 21 931.5 22 1044.0 23 1124.5 24 1262.5 25 1490.5 26 1770.5 27 2038.0 28 2311.0 29 2679.0 30 3156.0 31 3566.5 32 3966.5 33 4367.0 34 4788.5 35 5336.5 36 6042.5 37 6410.0 38 6446.0 39 6446.5 40 6469.0 41 6569.5 42 6430.0 43 6550.5 44 6739.5 45 6721.5 46 6916.5 47 7242.5 48 9294.5 49 9094.5 50 6797.0 51 6167.0 52 5643.5 53 5439.5 54 5129.5 55 4679.0 56 4161.0 57 3657.0 58 3341.0 59 3007.5 60 2424.0 61 1904.5 62 1534.0 63 1152.0 64 799.0 65 554.5 66 385.5 67 317.0 68 251.5 69 151.5 70 113.0 71 80.5 72 59.0 73 46.5 74 30.0 75 20.0 76 13.5 77 9.0 78 5.0 79 3.5 80 2.0 81 0.5 82 0.5 83 0.5 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02229784098730962 2 9.694713472743312E-4 3 0.002423678368185828 4 0.0 5 0.002423678368185828 6 4.847356736371656E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.423678368185828E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0016965748577300798 22-23 0.0 24-25 0.001211839184092914 26-27 0.0016965748577300798 28-29 0.0016965748577300798 30-31 0.011876024004110558 32-33 2.423678368185828E-4 34-35 0.0016965748577300798 36-37 0.002423678368185828 38-39 0.001211839184092914 40-41 0.0036355175522787427 42-43 9.694713472743312E-4 44-45 2.423678368185828E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0016965748577300798 54-55 0.0031507818786415767 56-57 4.847356736371656E-4 58-59 0.001211839184092914 60-61 0.001454207020911497 62-63 0.0 64-65 2.423678368185828E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 2.423678368185828E-4 76-77 2.423678368185828E-4 78-79 0.0 80-81 0.005332092410008822 82-83 0.0016965748577300798 84-85 0.002908414041822994 86-87 0.0019389426945486624 88-89 4.847356736371656E-4 90-91 0.0 92-93 2.423678368185828E-4 94-95 0.0 96-97 0.0021813105313672454 98-99 0.0016965748577300798 100-101 9.694713472743312E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 206298.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.23965125461562 #Duplication Level Percentage of deduplicated Percentage of total 1 83.48858813328485 53.63277785422459 2 10.288159045441297 13.218154982623362 3 2.683000054827802 5.170649635147577 4 1.1483393144176384 2.95075668320614 5 0.6194776501636716 1.9897514103271525 6 0.39590813154504484 1.5259800179592102 7 0.2800006045954073 1.2590998833203344 8 0.1675213419837086 0.860921006939091 9 0.13694840625928334 0.7917766072174602 >10 0.7201421462237756 8.175825542308948 >50 0.04236099128865871 1.9542410206004335 >100 0.02265820464277094 3.204752761761851 >500 0.003940557329177555 1.7378062961686498 >1k 0.002955417996883166 3.5275062981952128 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2330 1.129434119574596 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1945 0.9428108852242872 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1299 0.6296716400546781 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 821 0.397967988056113 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 697 0.33786076452510444 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 646 0.313139245169609 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 582 0.28211616205683043 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 486 0.23558153738766252 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 412 0.19971109753851224 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 371 0.17983693491938846 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 366 0.17741325655120263 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 355 0.17208116414119382 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 355 0.17208116414119382 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 253 0.1226381254302029 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 237 0.11488235465200826 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 210 0.10179449146380479 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 207 0.10034028444289328 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.8473567363716566E-4 0.0 24-25 0.0 0.0 0.0 0.0019389426945486629 0.0 26-27 0.0 0.0 0.0 0.0033931497154601597 0.0 28-29 0.0 0.0 0.0 0.008482874288650398 0.0 30-31 0.0 0.0 0.0 0.017692852087756547 0.0 32-33 0.0 0.0 0.0 0.02472151935549545 0.0 34-35 0.0 0.0 0.0 0.031023083112778606 0.0 36-37 0.0 0.0 0.0 0.04435331413780066 0.0 38-39 0.0 0.0 0.0 0.05695644165236696 0.0 40-41 0.0 0.0 0.0 0.07319508671921202 0.0 42-43 0.0 0.0 0.0 0.1114892049365481 0.0 44-45 0.0 0.0 0.0 0.15560015123753018 0.0 46-47 0.0 0.0 0.0 0.1914705910866804 0.0 48-49 0.0 0.0 0.0 0.24261020465540142 0.0 50-51 0.0 0.0 0.0 0.29859717496049404 0.0 52-53 0.0 0.0 0.0 0.3890003780938254 0.0 54-55 0.0 0.0 0.0 0.46389203967076753 0.0 56-57 0.0 0.0 0.0 0.6282174330337666 0.0 58-59 0.0 0.0 0.0 0.8640413382582478 0.0 60-61 0.0 0.0 0.0 1.099865243482729 0.0 62-63 0.0 0.0 0.0 1.4496020320119438 0.0 64-65 0.0 0.0 0.0 1.637194737709527 0.0 66-67 0.0 0.0 0.0 1.993960193506481 0.0 68-69 0.0 0.0 0.0 2.4011381593617003 0.0 70-71 0.0 0.0 0.0 2.713065565347216 0.0 72-73 0.0 0.0 0.0 3.3376474808287044 0.0 74-75 0.0 0.0 0.0 3.8810361709759666 0.0 76-77 0.0 0.0 0.0 4.209929325538784 0.0 78-79 0.0 0.0 0.0 4.522826202871574 0.0 80-81 0.0 0.0 0.0 5.138440508390774 0.0 82-83 0.0 0.0 0.0 5.5080514595391135 0.0 84-85 0.0 0.0 0.0 6.167534343522478 0.0 86-87 0.0 0.0 0.0 7.513645309212887 0.0 88-89 0.0 0.0 0.0 9.121755906504184 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTAGC 40 5.3808856E-4 47.49455 7 GTGTAGC 55 4.9382896E-5 43.197803 1 CTAGTAG 45 9.6006494E-4 42.22761 3 CGTAGCT 45 9.6121174E-4 42.217373 8 TCTACCC 55 0.0025731286 34.549862 3 TATTCGT 55 0.0025731286 34.549862 4 ATATTCG 55 0.0025731286 34.549862 3 CTAGGAC 135 1.1856173E-8 31.670708 3 GATAGCT 60 0.0039410405 31.670706 4 CTGCCGC 30 0.0039502624 31.663029 84-85 TAAGGTA 65 0.005829466 29.2345 4 TACCTGG 115 3.8405615E-6 28.916733 2 CAATAAG 120 5.3382428E-6 27.718588 1 TGCGCGC 35 0.008370313 27.152906 26-27 TTCGTAG 70 0.008380585 27.13974 6 ATGTAAG 95 0.0012205058 25.009254 1 GCTTATA 115 1.273569E-4 24.791782 1 AATAAGG 140 1.768563E-5 23.753033 2 TTATACT 150 3.0146972E-5 22.169495 3 GTACATG 1375 0.0 22.117275 1 >>END_MODULE