FastQCFastQC Report
Thu 26 May 2016
SRR936926_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936926_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125023
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16281.3021604024859426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11790.9430264831271046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8290.6630779936491685No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4770.38152979851707286No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3900.31194260256112877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.2911464290570535No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3630.2903465762299737No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2680.21436055765739104No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2640.2111611463490718No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2550.2039624709053534No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.19356438415331578No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.18316629740127816No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2070.16556953520552217No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2020.16157027107012312No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1380.1103796901370148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC200.002062656271.311583
TCCGGTG250.004993722357.0492632
CTAAGGT352.7664765E-454.3326264
GTTATGC352.7664765E-454.3326261
ACAGCCC459.5702556E-442.241798
GAGGGCA756.851078E-638.0176169
GTATCAA13550.035.0856441
TAAGCTT550.002559996734.575315
CATGGGG2000.033.2787364
TACAGCA600.00392867731.6813437
TATCAAC15950.029.8063012
ATGGGGC804.4484233E-429.7131585
CATACAC804.4484233E-429.7131583
ATACACT804.4484233E-429.7131584
GGTATCA8650.029.6788061
ATATATG650.005799866729.256031
GGTACAG650.005811234529.244328
AGAGGGC650.005811234529.244328
ATCAACG16400.028.9884453
CAACGCA16500.028.5246285
Produced by FastQC (version 0.11.5)