##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125023 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01737280340417 33.0 31.0 34.0 30.0 34.0 2 32.122433472241106 33.0 31.0 34.0 30.0 34.0 3 32.12168161058365 34.0 31.0 34.0 30.0 34.0 4 35.69053694120282 37.0 35.0 37.0 35.0 37.0 5 35.69475216560153 37.0 35.0 37.0 35.0 37.0 6 35.7816161826224 37.0 35.0 37.0 35.0 37.0 7 35.73972789006823 37.0 35.0 37.0 35.0 37.0 8 35.68844132679587 37.0 35.0 37.0 35.0 37.0 9 37.36545275669277 39.0 37.0 39.0 35.0 39.0 10-11 37.209861385505064 39.0 37.0 39.0 34.0 39.0 12-13 37.179778920678594 39.0 37.0 39.0 34.0 39.0 14-15 38.33980547579246 40.0 38.0 41.0 34.0 41.0 16-17 38.30349615670717 40.0 38.0 41.0 34.0 41.0 18-19 38.35643841533158 40.0 38.0 41.0 34.0 41.0 20-21 38.330211241131636 40.0 38.0 41.0 34.0 41.0 22-23 38.22908184893979 40.0 38.0 41.0 34.0 41.0 24-25 38.08692800524703 40.0 38.0 41.0 34.0 41.0 26-27 38.097118130264036 40.0 38.0 41.0 34.0 41.0 28-29 38.02493941114835 40.0 38.0 41.0 34.0 41.0 30-31 37.799884821192904 40.0 37.5 41.0 33.5 41.0 32-33 37.484862785247515 40.0 36.5 41.0 33.0 41.0 34-35 37.2866672532254 40.0 36.0 41.0 33.0 41.0 36-37 37.047643233645005 40.0 35.5 41.0 32.0 41.0 38-39 36.842157043104066 39.0 35.0 41.0 31.5 41.0 40-41 36.58539628708318 39.0 35.0 41.0 31.0 41.0 42-43 36.29380593970709 39.0 35.0 41.0 30.0 41.0 44-45 35.85132735576654 39.0 35.0 41.0 29.5 41.0 46-47 35.271122113531106 39.0 35.0 40.5 26.0 41.0 48-49 34.76106796349471 39.0 35.0 40.0 23.5 41.0 50-51 34.04884301288563 38.0 34.0 40.0 21.5 40.5 52-53 34.16960479271814 38.5 34.5 40.0 21.0 40.5 54-55 34.49006182862354 39.0 35.0 40.5 19.5 41.0 56-57 34.364256976716284 39.0 35.0 41.0 17.5 41.0 58-59 34.11937003591339 39.0 35.0 41.0 13.5 41.0 60-61 33.81759756204858 38.0 34.0 40.5 8.5 41.0 62-63 33.49203746510642 38.0 34.0 40.0 4.5 41.0 64-65 33.09197507658591 37.5 34.0 40.0 2.0 41.0 66-67 32.697311694648185 37.0 33.0 40.0 2.0 41.0 68-69 32.29053854090847 36.5 33.0 39.5 2.0 41.0 70-71 31.82409236700447 36.0 32.5 39.0 2.0 41.0 72-73 31.381805747742415 35.5 32.0 39.0 2.0 40.5 74-75 30.923470081505002 35.0 31.0 38.0 2.0 40.0 76-77 30.464610511665853 35.0 31.0 37.0 2.0 39.5 78-79 30.02319973124945 35.0 31.0 37.0 2.0 39.0 80-81 29.60820409044736 35.0 30.0 36.0 2.0 39.0 82-83 29.182510418083073 35.0 30.0 36.0 2.0 37.5 84-85 28.830303224206745 34.0 29.0 35.0 2.0 37.0 86-87 28.458451644897337 34.0 29.0 35.0 2.0 37.0 88-89 28.086024171552435 34.0 29.0 35.0 2.0 36.0 90-91 27.714392551770473 34.0 27.5 35.0 2.0 36.0 92-93 27.373331307039503 34.0 27.0 35.0 2.0 35.5 94-95 26.995480831526997 34.0 26.0 35.0 2.0 35.0 96-97 26.526367148444685 34.0 25.0 35.0 2.0 35.0 98-99 25.948169536805228 34.0 23.5 35.0 2.0 35.0 100-101 24.030430400806253 31.5 10.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1710143446903487 1101 2 0.2865959323369296 1101 3 0.27777040255193697 1101 4 0.2896722832318588 1101 5 0.19106377441026723 1101 6 0.07994875475465335 1101 7 0.2013332169278783 1101 8 0.2645984460182973 1101 9 0.16216739509613376 1101 10-11 0.25595033516031407 1101 12-13 0.21171940681798418 1101 14-15 0.45622873493109495 1101 16-17 0.39526793959387163 1101 18-19 0.4227667584228314 1101 20-21 0.43227067632883376 1101 22-23 0.3067005904109479 1101 24-25 0.34800256917520045 1101 26-27 0.49158074260009954 1101 28-29 0.40664327322193117 1101 30-31 0.6363332961860451 1101 32-33 0.9540982214006632 1101 34-35 1.10280934510687 1101 36-37 0.9432377948065138 1101 38-39 0.7142860412611682 1101 40-41 1.0900846183320425 1101 42-43 1.1629421863628622 1101 44-45 0.7661473422699103 1101 46-47 1.0843637045821595 1101 48-49 1.3857529902725076 1101 50-51 1.8188156238120214 1101 52-53 2.0575107113806084 1101 54-55 2.0141343843706565 1101 56-57 2.145481718542541 1101 58-59 1.9328648445874066 1101 60-61 1.9190570553640995 1101 62-63 2.2198652034897393 1101 64-65 1.8315363500558064 1101 66-67 2.052327347150335 1101 68-69 1.949033937288636 1101 70-71 2.2687965692304566 1101 72-73 2.0946551961727415 1101 74-75 2.295933489175521 1101 76-77 2.523178839074326 1101 78-79 2.441719056532353 1101 80-81 2.4998530692850913 1101 82-83 2.3379198210330827 1101 84-85 2.6061350762977007 1101 86-87 2.0372008444617933 1101 88-89 2.385599056221725 1101 90-91 2.6269037693834925 1101 92-93 2.2984467699273985 1101 94-95 2.0027835588611644 1101 96-97 2.141390826219787 1101 98-99 2.764020206836765 1101 100-101 2.5770534107732033 1102 1 0.20849807772622597 1102 2 0.2278721918647264 1102 3 -8.362157786123703E-4 1102 4 0.11926421481765459 1102 5 -0.023148904526337333 1102 6 0.19528153036350204 1102 7 0.13194299569716605 1102 8 0.15298383363681012 1102 9 -0.10965357534446696 1102 10-11 0.26536193582958134 1102 12-13 0.19304725586055582 1102 14-15 0.21131797084855464 1102 16-17 0.3345340671268744 1102 18-19 0.10389940389595864 1102 20-21 -0.05911945194780088 1102 22-23 0.16530581443028325 1102 24-25 0.19275763517260458 1102 26-27 0.40392504451495626 1102 28-29 0.4540614736309223 1102 30-31 0.2640405837859774 1102 32-33 0.35554365735530524 1102 34-35 0.4331332900780538 1102 36-37 0.21479152226514486 1102 38-39 0.8301997798742917 1102 40-41 0.503300364753315 1102 42-43 0.72819595100313 1102 44-45 0.9681133210763875 1102 46-47 1.0833644482148017 1102 48-49 1.5188283760319479 1102 50-51 1.9622089125273945 1102 52-53 1.7224344890344483 1102 54-55 1.8229975559638874 1102 56-57 1.8935645406284323 1102 58-59 1.812419594540927 1102 60-61 1.873695463990238 1102 62-63 2.156761001965286 1102 64-65 2.3566688096207784 1102 66-67 2.8063359918798376 1102 68-69 2.8470749300382217 1102 70-71 2.9436896720376744 1102 72-73 2.7547918386713555 1102 74-75 3.194439252328525 1102 76-77 3.1466102389627792 1102 78-79 3.0593989226784757 1102 80-81 2.961811611765107 1102 82-83 2.347552187643977 1102 84-85 2.571161103440293 1102 86-87 2.24859004319762 1102 88-89 2.5561581750170426 1102 90-91 2.9455735964889023 1102 92-93 3.22538486250966 1102 94-95 2.6124112777680217 1102 96-97 2.5353069816390104 1102 98-99 2.5632393925590193 1102 100-101 2.953924111646973 1103 1 -0.1449365755550538 1103 2 -0.0937721658225783 1103 3 0.21335322463782802 1103 4 -0.04161606032642595 1103 5 0.3567079461894096 1103 6 -0.01821075444780007 1103 7 0.026486591161003048 1103 8 -0.04828498956452876 1103 9 0.14069500245809508 1103 10-11 0.10564358669405038 1103 12-13 0.34822247430264497 1103 14-15 -0.020765130099576368 1103 16-17 0.09158695799877847 1103 18-19 0.14362565412834982 1103 20-21 0.4798166885987669 1103 22-23 0.37878647998150683 1103 24-25 0.2605792562917699 1103 26-27 0.12348258309089744 1103 28-29 0.004802782424377483 1103 30-31 -0.12440290013297073 1103 32-33 0.31912889624729246 1103 34-35 0.3267357254749683 1103 36-37 -0.18713030335300118 1103 38-39 -0.22129678082472282 1103 40-41 -0.250406179213968 1103 42-43 0.3899360513935406 1103 44-45 0.7324050109815659 1103 46-47 1.047553888631242 1103 48-49 1.0207223154258855 1103 50-51 0.6209628630758246 1103 52-53 0.5697806500309142 1103 54-55 0.6935822371313876 1103 56-57 0.9706350927756731 1103 58-59 0.845441531703969 1103 60-61 0.6981981596585811 1103 62-63 0.9652639764958693 1103 64-65 1.0661989267429206 1103 66-67 1.187296672303706 1103 68-69 1.450567679619926 1103 70-71 1.555606385181374 1103 72-73 1.3753300427985131 1103 74-75 1.0045224462307338 1103 76-77 0.8713383482767796 1103 78-79 1.5460135350599593 1103 80-81 1.5366628852360122 1103 82-83 1.2198216615238806 1103 84-85 1.402147346236351 1103 86-87 1.6046855070384787 1103 88-89 1.206151203460987 1103 90-91 0.651443646684104 1103 92-93 0.6634774447740206 1103 94-95 0.7389798778795686 1103 96-97 1.4450718078148803 1103 98-99 1.6014435197202026 1103 100-101 2.2549675212026514 1104 1 0.2569568029090874 1104 2 -0.23699196696495406 1104 3 0.14401749373027428 1104 4 0.2202307769911087 1104 5 0.014207417312739778 1104 6 -0.002080016135977303 1104 7 -0.4242221035713811 1104 8 0.11889292454590361 1104 9 -0.26521783866735404 1104 10-11 0.05611428692676412 1104 12-13 0.12062040110610894 1104 14-15 0.4644527670867973 1104 16-17 0.03235277141109094 1104 18-19 0.05263242374967092 1104 20-21 0.3505592314377779 1104 22-23 0.032795365109485886 1104 24-25 0.021976548443241484 1104 26-27 -0.4727729701140859 1104 28-29 -0.2794155378633292 1104 30-31 0.1926586611109471 1104 32-33 0.4418811704169556 1104 34-35 0.32543997764336297 1104 36-37 0.267756517040489 1104 38-39 0.41079016649916156 1104 40-41 0.03706473256940512 1104 42-43 -0.09586361012539868 1104 44-45 0.7451509386312409 1104 46-47 1.3273560887581723 1104 48-49 1.606929211977608 1104 50-51 1.4213912531752513 1104 52-53 1.838291333339562 1104 54-55 1.6483632821916885 1104 56-57 1.6069266095939554 1104 58-59 1.7525972329944324 1104 60-61 2.2586223187446848 1104 62-63 2.070880646688387 1104 64-65 1.6538866779593349 1104 66-67 1.2763595028516228 1104 68-69 1.2669417011980961 1104 70-71 1.9431672059979626 1104 72-73 2.0494626850663344 1104 74-75 1.660805501022356 1104 76-77 1.7698469160787695 1104 78-79 2.055480427380669 1104 80-81 2.1351397204380405 1104 82-83 2.0905642043628916 1104 84-85 2.2789719707339167 1104 86-87 0.6847838780983508 1104 88-89 1.0806487706283257 1104 90-91 0.9753541607522216 1104 92-93 0.6630807872745521 1104 94-95 0.9554755410909088 1104 96-97 0.6200770965815394 1104 98-99 0.7168444082330012 1104 100-101 1.176429336307372 1105 1 0.47098404854553166 1105 2 0.6710540100465394 1105 3 0.8691132791708824 1105 4 0.8752967625168679 1105 5 0.7885794905353904 1105 6 0.899686693101998 1105 7 0.7590473728015397 1105 8 0.9121028010891123 1105 9 0.550980543622984 1105 10-11 1.1103394891404648 1105 12-13 0.7796774466574163 1105 14-15 1.220998105528686 1105 16-17 0.7909734610662653 1105 18-19 0.7415958124257074 1105 20-21 0.8976005256055117 1105 22-23 0.6998877448455474 1105 24-25 0.4658356374172712 1105 26-27 1.0778868851434638 1105 28-29 0.5319935723963596 1105 30-31 0.3353789676243579 1105 32-33 0.7176368111151277 1105 34-35 0.844118138562898 1105 36-37 0.24572586569949095 1105 38-39 0.7000089830168221 1105 40-41 0.9654917227779976 1105 42-43 0.9908222136293929 1105 44-45 1.2146060259024196 1105 46-47 1.3020793752630624 1105 48-49 1.1812161425863792 1105 50-51 1.0519255230773368 1105 52-53 0.8928890344889879 1105 54-55 0.7661793741457075 1105 56-57 1.5748917011222616 1105 58-59 1.8518415922962603 1105 60-61 1.7137628040575805 1105 62-63 1.5203272196981743 1105 64-65 1.5913068567588269 1105 66-67 1.4575004138776002 1105 68-69 1.7384189255488813 1105 70-71 2.1372923656403593 1105 72-73 2.632317091196601 1105 74-75 2.6853904307797016 1105 76-77 2.7367477249336005 1105 78-79 2.539196902476455 1105 80-81 2.1415254164789133 1105 82-83 2.0450129059380266 1105 84-85 1.37741811803064 1105 86-87 1.8568251722660811 1105 88-89 2.1446121593043586 1105 90-91 1.7450966043452425 1105 92-93 1.4066418771000109 1105 94-95 1.6593388871956343 1105 96-97 1.311541549540248 1105 98-99 2.0267635227498175 1105 100-101 2.142265862488724 1106 1 -0.23488849066034234 1106 2 0.18006301905555233 1106 3 -0.1819377718801647 1106 4 -0.7166284960750602 1106 5 -0.4308065621839958 1106 6 -0.4473269872450132 1106 7 -0.5807924670383002 1106 8 -0.3067827511297736 1106 9 -0.42015726084815697 1106 10-11 0.15571820118584156 1106 12-13 0.43224668255997756 1106 14-15 0.21899610352668475 1106 16-17 0.432865352958153 1106 18-19 0.21164919914576075 1106 20-21 -0.09305679838514891 1106 22-23 -0.23421302258855548 1106 24-25 -0.7274409725260043 1106 26-27 -0.5237981332082242 1106 28-29 0.08638129345074219 1106 30-31 0.046339928585318546 1106 32-33 -0.44861277180111614 1106 34-35 -0.9216476272028657 1106 36-37 -0.27604590607228374 1106 38-39 -0.15526295558844794 1106 40-41 -0.5772176532647109 1106 42-43 -0.825243852436671 1106 44-45 -1.0125377845747252 1106 46-47 -1.4896289997786383 1106 48-49 -1.1617101170065496 1106 50-51 -0.5125556247371392 1106 52-53 -1.1521560105560553 1106 54-55 -1.4725593645930317 1106 56-57 -1.2905570809931888 1106 58-59 -0.725152068030738 1106 60-61 -0.5800309897362155 1106 62-63 -0.8130895303852483 1106 64-65 -0.8796641142121473 1106 66-67 -1.6532770302331805 1106 68-69 -1.0597125392492508 1106 70-71 -0.8599548816068854 1106 72-73 -0.07413936525985321 1106 74-75 0.028233273622543464 1106 76-77 -0.2911968863443448 1106 78-79 -0.9240163107367572 1106 80-81 -0.8159320409785451 1106 82-83 -0.5489977714059826 1106 84-85 -0.5173933601141734 1106 86-87 -0.30275107064349527 1106 88-89 0.08088176224062593 1106 90-91 0.5134482893635912 1106 92-93 0.4059745430993438 1106 94-95 0.5867663146230591 1106 96-97 0.685081756413787 1106 98-99 0.47663005594968055 1106 100-101 0.26939298961585223 1107 1 -0.6132668690387213 1107 2 -0.4820991431066126 1107 3 -0.6278837178261121 1107 4 -1.0679798474264075 1107 5 -0.47134710272453617 1107 6 -0.5554350953531255 1107 7 -0.6483600346058651 1107 8 -0.4554313997784192 1107 9 -0.5147518554427464 1107 10-11 -0.5469845015168531 1107 12-13 -0.4596452093319101 1107 14-15 -0.5107336262030415 1107 16-17 -0.5468643767715733 1107 18-19 -0.5721345846380288 1107 20-21 -0.6065703118986576 1107 22-23 -0.4301589685344993 1107 24-25 -0.558522053607085 1107 26-27 -1.0778521872622733 1107 28-29 -0.45415924708979105 1107 30-31 -0.548254666009278 1107 32-33 -0.7796938528822039 1107 34-35 -1.1108368163920517 1107 36-37 -1.6409107709371469 1107 38-39 -1.3579656582911426 1107 40-41 -0.7461365721836302 1107 42-43 -1.0482168254096464 1107 44-45 -1.3030783251152656 1107 46-47 -1.3612506214002664 1107 48-49 -1.6008993061957426 1107 50-51 -1.032825895007413 1107 52-53 -1.2737776321776764 1107 54-55 -0.9590458510795159 1107 56-57 -1.459475999912108 1107 58-59 -1.1778547707334326 1107 60-61 -1.113814773519998 1107 62-63 -0.6576841249798377 1107 64-65 -0.8256100601580911 1107 66-67 -1.2613851383412928 1107 68-69 -1.5664692960060087 1107 70-71 -1.4815765032285064 1107 72-73 -0.5741393652598497 1107 74-75 -0.8704153750261021 1107 76-77 -0.7101158052632641 1107 78-79 -0.6942865810070309 1107 80-81 -0.8767428517893592 1107 82-83 -0.7854842578924668 1107 84-85 -0.7741501168709313 1107 86-87 -1.045994313886741 1107 88-89 -0.7772263458674793 1107 90-91 -0.5676327917174895 1107 92-93 -0.6210524839276843 1107 94-95 -1.3186390907823444 1107 96-97 -1.1122155408835113 1107 98-99 -0.6787753494557229 1107 100-101 0.5464200166428768 1108 1 0.15054918582210775 1108 2 0.7805067463649493 1108 3 0.33843334550587656 1108 4 0.2841782808511226 1108 5 0.2591894039313729 1108 6 0.7152385633802325 1108 7 0.7695504131553292 1108 8 0.5629227752921722 1108 9 0.5848850949404678 1108 10-11 -0.18494335589362265 1108 12-13 0.17639754984517708 1108 14-15 0.2315011458824756 1108 16-17 0.5148540580811911 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-1.280602109270081 2316 98-99 -1.924639395319769 2316 100-101 -2.278272858049995 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 405.0 3 122.0 4 103.0 5 79.0 6 89.0 7 108.0 8 136.0 9 171.0 10 212.0 11 319.0 12 503.0 13 669.0 14 801.0 15 1089.0 16 1433.0 17 2192.0 18 1994.0 19 1074.0 20 996.0 21 956.0 22 977.0 23 1070.0 24 1131.0 25 1240.0 26 1300.0 27 1450.0 28 1540.0 29 1791.0 30 2173.0 31 2779.0 32 3468.0 33 4588.0 34 6728.0 35 10160.0 36 15479.0 37 24359.0 38 25456.0 39 5878.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.11889426731207 19.372724726967235 15.861103332399887 20.6472776733208 2 14.55248061829441 21.194665130531487 42.18051188504589 22.07234236612822 3 18.47644343464243 27.703064873556965 31.438354520508494 22.38213717129211 4 11.587766468215863 17.67092375648166 39.70376416362589 31.037545611676588 5 11.97464362608953 38.027357350386986 35.18356958195599 14.814429441567484 6 27.39707388910232 38.30596107055961 19.147778204635678 15.149186835702395 7 24.685271590809048 35.43365693202827 22.44835893044362 17.432712546719063 8 24.86913928062621 33.67562549022747 21.309087416561283 20.14614781258504 9 26.169952217446635 17.016831944677886 21.736659703379996 35.07655613449548 10-11 22.92703034765939 27.440959535120125 28.371839869372593 21.26017024784789 12-13 25.42501080535946 25.630312635066993 28.98917863260177 19.955497926971777 14-15 22.411364545818326 26.026810724289717 29.244497799119646 22.317326930772307 16-17 19.283433501124385 29.450530982658034 29.64179677809166 21.624238738125914 18-19 19.08375874153851 27.31160684280433 34.18201603482213 19.422618380835026 20-21 20.136204675133442 26.3550444545811 34.00355316543827 19.50519770484719 22-23 19.674118287140754 26.326169749727967 33.80392690264354 20.195785060487744 24-25 19.39927759711039 26.34450537802151 34.37973751895008 19.876479505918024 26-27 19.401325499262057 26.458789132025966 34.25459665065455 19.885288718057428 28-29 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28.130799257695017 31.58795674153708 20.63455877647661 54-55 19.451888233835422 27.992336674372652 31.8168001215893 20.738974970202623 56-57 19.478176698345653 28.873476048766438 30.840213113180376 20.80813413970753 58-59 19.534212168237158 27.89287599788824 31.399683235477628 21.173228598396978 60-61 19.39255514044112 27.353818830550647 31.928655429243435 21.324970599764796 62-63 20.147268807570626 27.101540282938497 31.781584599570433 20.969606309920447 64-65 19.88849293481956 27.22404201112671 32.14507117174408 20.74239388230965 66-67 19.304196181798545 27.278181534560204 31.974337334458607 21.443284949182644 68-69 19.166586669226586 27.453121500111997 32.314965921090526 21.065325909570895 70-71 19.223769314768223 27.531269624764505 31.53842153894153 21.70653952152574 72-73 18.95802083958321 27.1502569948601 31.305373892522148 22.586348273034538 74-75 19.286828526858926 26.838926442942284 31.967121315147395 21.907123715051398 76-77 19.497501910068763 26.821765583561007 31.77914404918577 21.90158845718446 78-79 18.836839936469072 26.468152522233822 31.98870205675376 22.70630548454334 80-81 18.90857156573294 26.99607146571934 31.989550578878728 22.105806389668995 82-83 19.189563297114784 27.102302731593348 31.43772703269271 22.27040693859916 84-85 18.867554702188087 26.472258890355615 30.870434817392695 23.789751590063602 86-87 19.037371551541383 26.431682819806163 31.11002667967984 23.420918948972613 88-89 19.697036364436375 25.20110241322896 31.835582026984323 23.266279195350346 90-91 18.88926664400136 25.13289202647841 32.87922724636522 23.098614083155013 92-93 18.449340123454324 25.75218725372143 32.0945229646639 23.703949658160347 94-95 18.027239455210893 26.423471530569387 31.976560468790627 23.572728545429094 96-97 18.03593504326848 26.645622542018238 31.75342367103952 23.56501874367376 98-99 18.599234869463608 27.775198885918716 30.173434923887122 23.452131320730555 100-101 18.610384101295814 27.80352326298013 29.092131486061422 24.493961149662642 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 131.0 1 98.5 2 87.0 3 104.5 4 85.5 5 77.0 6 125.0 7 304.0 8 564.0 9 654.0 10 509.5 11 377.0 12 375.5 13 387.0 14 381.0 15 400.5 16 410.0 17 423.5 18 457.5 19 493.0 20 530.5 21 597.5 22 671.0 23 766.5 24 860.0 25 996.0 26 1210.0 27 1465.0 28 1631.5 29 1784.0 30 2051.0 31 2270.5 32 2548.0 33 2884.0 34 3176.0 35 3364.0 36 3686.5 37 4040.0 38 4104.5 39 4021.0 40 3923.0 41 3962.5 42 3977.5 43 3978.0 44 4100.5 45 4185.5 46 4259.5 47 4436.0 48 5623.5 49 5498.5 50 4080.0 51 3605.0 52 3310.5 53 3158.5 54 2914.0 55 2637.0 56 2344.0 57 1996.0 58 1764.0 59 1517.0 60 1150.0 61 934.5 62 755.5 63 529.0 64 371.5 65 270.0 66 197.0 67 155.5 68 111.0 69 72.0 70 54.0 71 31.5 72 20.0 73 18.0 74 13.0 75 10.0 76 6.0 77 4.0 78 4.0 79 4.0 80 2.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030394407429033057 2 0.02719499612071379 3 0.020796173504075248 4 0.04399190548938995 5 0.06718763747470465 6 0.06318837333930556 7 0.059189109203906486 8 0.06478807899346521 9 0.06558793182054502 10-11 0.06998712236948401 12-13 0.06798749030178447 14-15 0.05838925637682667 16-17 0.05279028658726795 18-19 0.03599337721859178 20-21 0.051190580933108305 22-23 0.031194260256112875 24-25 0.018796541436375706 26-27 0.010798013165577533 28-29 0.013197571646816987 30-31 0.009598233924957808 32-33 0.005998896203098629 34-35 0.007998528270798173 36-37 0.005199043376018812 38-39 0.005998896203098629 40-41 0.005998896203098629 42-43 0.005199043376018812 44-45 0.001199779240619726 46-47 0.004399190548938995 48-49 7.998528270798173E-4 50-51 0.005598969789558721 52-53 0.005598969789558721 54-55 0.009598233924957808 56-57 0.015197203714516529 58-59 0.007198675443718355 60-61 0.019196467849915615 62-63 0.010798013165577533 64-65 0.006798749030178447 66-67 0.013197571646816987 68-69 0.015197203714516529 70-71 0.017196835782216074 72-73 0.016396982955136256 74-75 0.01439735088743671 76-77 0.021995952744694975 78-79 0.03559345080505187 80-81 0.03199411308319269 82-83 0.03279396591027251 84-85 0.022395879158234884 86-87 0.017196835782216074 88-89 0.019596394263455524 90-91 0.012397718819737169 92-93 0.02999448101549315 94-95 0.016396982955136256 96-97 0.03799300928629132 98-99 0.06158866768514594 100-101 0.06558793182054502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 125023.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.7575086184142 #Duplication Level Percentage of deduplicated Percentage of total 1 91.74122784745849 72.25310542860115 2 5.294266998425837 8.339265575134174 3 1.2877672269334282 3.042640154211625 4 0.5707611841771187 1.7980691552754293 5 0.3006144315238918 1.1837821840781295 6 0.2000710912506982 0.9454260416083441 7 0.10765246534301529 0.5934907976932244 8 0.09749657238612705 0.6142869711972997 9 0.06296653633270706 0.4463178775105381 >10 0.30264561011526936 3.958471641218016 >50 0.014218250139643528 0.7406637178759108 >100 0.01726501802671 3.1762155763339543 >500 0.0010155892956888236 0.6630779936491685 >1k 0.002031178591377647 2.2451868856130472 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1628 1.3021604024859426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1179 0.9430264831271046 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 829 0.6630779936491685 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 477 0.38152979851707286 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 390 0.31194260256112877 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 364 0.2911464290570535 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 363 0.2903465762299737 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 268 0.21436055765739104 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 264 0.2111611463490718 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 255 0.2039624709053534 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.19356438415331578 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.18316629740127816 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 207 0.16556953520552217 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.16157027107012312 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 138 0.1103796901370148 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.998528270798173E-4 0.0 10-11 0.0 0.0 0.0 7.998528270798173E-4 0.0 12-13 0.0 0.0 0.0 7.998528270798173E-4 0.0 14-15 0.0 0.0 0.0 7.998528270798173E-4 0.0 16-17 0.0 0.0 0.0 0.0015997056541596345 0.0 18-19 0.0 0.0 0.0 0.0015997056541596345 0.0 20-21 0.0 0.0 0.0 0.0019996320676995433 0.0 22-23 0.0 0.0 0.0 0.004399190548938996 0.0 24-25 0.0 0.0 0.0 0.006398822616638538 0.0 26-27 0.0 0.0 0.0 0.009598233924957808 0.0 28-29 0.0 0.0 0.0 0.014397350887436712 0.0 30-31 0.0 0.0 0.0 0.02719499612071379 0.0 32-33 0.0 0.0 0.0 0.040792494181070685 0.0 34-35 0.0 0.0 0.0 0.04999080169248858 0.0 36-37 0.0 0.0 0.0 0.06358829975284548 0.0 38-39 0.0 0.0 0.0 0.078785503467362 0.0 40-41 0.0 0.0 0.0 0.09798197131727762 0.0 42-43 0.0 0.0 0.0 0.15517144845348455 0.0 44-45 0.0 0.0 0.0 0.21955960103340985 0.0 46-47 0.0 0.0 0.0 0.28034841589147597 0.0 48-49 0.0 0.0 0.0 0.34993561184742006 0.0 50-51 0.0 0.0 0.0 0.41712324932212475 0.0 52-53 0.0 0.0 0.0 0.5794933732193277 0.0 54-55 0.0 0.0 0.0 0.70027115010838 0.0 56-57 0.0 0.0 0.0 0.9486254529166633 0.0 58-59 0.0 0.0 0.0 1.299760844004703 0.0 60-61 0.0 0.0 0.0 1.6197019748366301 0.0 62-63 0.0 0.0 0.0 2.1779992481383426 0.0 64-65 0.0 0.0 0.0 2.467945897954776 0.0 66-67 0.0 0.0 0.0 2.9890500147972774 0.0 68-69 0.0 0.0 0.0 3.6009374275133377 0.0 70-71 0.0 0.0 0.0 4.076449933212289 0.0 72-73 0.0 0.0 0.0 5.0282747974372715 0.0 74-75 0.0 0.0 0.0 5.830527182998328 0.0 76-77 0.0 0.0 0.0 6.286843220847365 0.0 78-79 0.0 0.0 0.0 6.762755652959855 0.0 80-81 0.0 0.0 0.0 7.744974924613871 0.0 82-83 0.0 0.0 0.0 8.23088551706486 0.0 84-85 0.0 0.0 0.0 9.15711509082329 0.0 86-87 0.0 0.0 0.0 11.177543332026907 0.0 88-89 0.0 0.0 0.0 13.460723226926245 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGTC 20 0.0020626562 71.31158 3 TCCGGTG 25 0.0049937223 57.049263 2 CTAAGGT 35 2.7664765E-4 54.332626 4 GTTATGC 35 2.7664765E-4 54.332626 1 ACAGCCC 45 9.5702556E-4 42.24179 8 GAGGGCA 75 6.851078E-6 38.017616 9 GTATCAA 1355 0.0 35.085644 1 TAAGCTT 55 0.0025599967 34.57531 5 CATGGGG 200 0.0 33.278736 4 TACAGCA 60 0.003928677 31.681343 7 TATCAAC 1595 0.0 29.806301 2 ATGGGGC 80 4.4484233E-4 29.713158 5 CATACAC 80 4.4484233E-4 29.713158 3 ATACACT 80 4.4484233E-4 29.713158 4 GGTATCA 865 0.0 29.678806 1 ATATATG 65 0.0057998667 29.25603 1 GGTACAG 65 0.0058112345 29.24432 8 AGAGGGC 65 0.0058112345 29.24432 8 ATCAACG 1640 0.0 28.988445 3 CAACGCA 1650 0.0 28.524628 5 >>END_MODULE