FastQCFastQC Report
Thu 26 May 2016
SRR936926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125023
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14471.1573870407844955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11890.9510250113979027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7970.6374827031826144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5700.4559161114354959No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4550.3639330363213169No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4410.35273509674219944No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG3780.3023443686361709No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3390.2711501083800581No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG2990.23915599529686538No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG2940.2351567311614663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.22155923310110942No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.19916335394287452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.1767674747846396No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA1830.14637306735560657No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1520.12157762971613223No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA1400.11197939579117443No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1260.10078145621205697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGACAT250.00501309656.9935157
GGGATAG250.00501309656.9935157
GTTGGAA352.779878E-454.279548
TATTCGT405.372669E-447.49464
GTAAGGT405.372669E-447.49464
ATATTCG405.372669E-447.49463
TCGTAGC459.597503E-442.2174227
GTAGCTT756.8783083E-637.995689
CGTAGCT500.00161121137.9956788
TGGACAG550.002572310234.5415275
TTCGTAG550.002572310234.5415276
AATGCGT352.0501245E-433.92471384-85
ATGGGAC1509.385985E-1031.6630675
GGTGTGC600.003939803731.6630678
AAGGTGT600.003939803731.6630676
ATGCGTA300.003947288331.66306784-85
TGCGTAG300.003947288331.66306786-87
TAAGGTG650.00582766429.2274485
TTTCGTC350.00836753427.1506364-65
CTACTCC700.0083681327.139774
Produced by FastQC (version 0.11.5)