##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 125023 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37977812082577 33.0 31.0 34.0 31.0 34.0 2 32.45229277812882 34.0 31.0 34.0 31.0 34.0 3 32.56420818569383 34.0 31.0 34.0 31.0 34.0 4 36.19919534805596 37.0 35.0 37.0 35.0 37.0 5 36.13896643017685 37.0 35.0 37.0 35.0 37.0 6 36.20073106548395 37.0 36.0 37.0 35.0 37.0 7 36.16155427401358 37.0 36.0 37.0 35.0 37.0 8 36.159762603680925 37.0 36.0 37.0 35.0 37.0 9 37.88855650560297 39.0 38.0 39.0 35.0 39.0 10-11 37.79918095070507 39.0 37.5 39.0 35.0 39.0 12-13 37.726818265439164 39.0 37.0 39.0 35.0 39.0 14-15 38.928785103540946 40.0 38.0 41.0 35.5 41.0 16-17 38.895903153819695 40.0 38.0 41.0 35.5 41.0 18-19 38.972781008294476 40.0 38.5 41.0 35.5 41.0 20-21 39.05270630204043 40.0 38.0 41.0 35.0 41.0 22-23 39.00233157099093 40.0 38.0 41.0 35.0 41.0 24-25 38.90363373139343 40.0 38.0 41.0 35.0 41.0 26-27 38.700035193524386 40.0 38.0 41.0 35.0 41.0 28-29 38.52104412788047 40.0 38.0 41.0 34.5 41.0 30-31 38.39550722667029 40.0 38.0 41.0 35.0 41.0 32-33 38.19419626788671 40.0 37.0 41.0 34.0 41.0 34-35 38.042976092399 40.0 37.0 41.0 34.0 41.0 36-37 37.83890564136199 40.0 37.0 41.0 33.5 41.0 38-39 37.62942018668565 40.0 36.5 41.0 33.0 41.0 40-41 37.334858386056965 40.0 36.0 41.0 33.0 41.0 42-43 37.033941754717134 40.0 36.0 41.0 32.5 41.0 44-45 36.613755069067295 40.0 35.5 41.0 31.5 41.0 46-47 36.03727714100606 40.0 35.0 41.0 29.5 41.0 48-49 35.472541052446346 39.0 35.0 41.0 27.0 41.0 50-51 35.1494005103061 39.0 35.0 41.0 25.5 41.0 52-53 34.849435703830494 39.0 35.0 40.5 24.0 41.0 54-55 34.54597154123641 39.0 35.0 40.0 23.0 41.0 56-57 34.278420770578215 38.0 34.5 40.0 21.5 41.0 58-59 33.92964094606592 38.0 34.0 40.0 19.5 41.0 60-61 33.58092111051566 38.0 34.0 40.0 16.5 41.0 62-63 33.21835182326452 37.0 33.5 40.0 11.5 41.0 64-65 32.751905649360516 36.5 33.0 39.5 7.5 41.0 66-67 32.29641745918751 36.0 32.5 39.0 2.0 40.5 68-69 31.849539684698016 35.5 32.0 39.0 2.0 40.0 70-71 31.435071946761795 35.0 31.5 38.0 2.0 40.0 72-73 31.044951728881884 35.0 31.0 38.0 2.0 40.0 74-75 30.543164057813364 35.0 31.0 37.0 2.0 39.0 76-77 29.105748542268223 33.5 28.5 35.5 2.0 38.5 78-79 29.677179398990585 34.0 30.0 36.0 2.0 38.5 80-81 29.219667581165062 34.0 29.0 36.0 2.0 37.5 82-83 29.134275293346022 34.0 30.0 35.0 2.0 37.0 84-85 28.86477288178975 34.0 29.5 35.0 2.0 37.0 86-87 28.52297577245787 34.0 29.0 35.0 2.0 36.0 88-89 28.137622677427352 34.0 29.0 35.0 2.0 36.0 90-91 27.722755013077595 34.0 28.5 35.0 2.0 35.5 92-93 27.374303128224405 34.0 27.0 35.0 2.0 35.0 94-95 26.96067523575662 33.5 26.5 35.0 2.0 35.0 96-97 26.42415795493629 33.0 25.0 35.0 2.0 35.0 98-99 25.797173320109103 33.0 23.5 35.0 2.0 35.0 100-101 23.05505786935204 29.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3547641432947657 1101 2 0.20059435436045447 1101 3 0.2739759157756083 1101 4 0.26100598586613444 1101 5 0.32939997159660805 1101 6 0.20554438905751482 1101 7 0.10278348627385725 1101 8 0.12306020875799817 1101 9 0.2015947392326609 1101 10-11 0.26226979789363014 1101 12-13 0.3923372240881591 1101 14-15 0.40124268096030136 1101 16-17 0.2668590824148964 1101 18-19 0.2943084863557317 1101 20-21 0.4142033437076833 1101 22-23 0.3211489606639546 1101 24-25 0.5452510805353228 1101 26-27 0.2720337244053752 1101 28-29 0.3175608949388362 1101 30-31 0.09719909792291048 1101 32-33 0.2787515199554491 1101 34-35 0.57508597507573 1101 36-37 0.36179630775449567 1101 38-39 0.2907151852214085 1101 40-41 0.4498934757183335 1101 42-43 0.4404622678567236 1101 44-45 0.49245632046432064 1101 46-47 0.5302846827975785 1101 48-49 0.5017396724183172 1101 50-51 0.39930454644322566 1101 52-53 0.21837882988192803 1101 54-55 0.21941766305915422 1101 56-57 0.3210245253050701 1101 58-59 0.3658336319243318 1101 60-61 0.45335626702606646 1101 62-63 0.5355824078914324 1101 64-65 0.7071047751053285 1101 66-67 0.5545367928473439 1101 68-69 0.5431033115829358 1101 70-71 0.7675131255731706 1101 72-73 0.5464913641857763 1101 74-75 0.5191009262071091 1101 76-77 0.2090546231668604 1101 78-79 0.8434279595294463 1101 80-81 0.966060197006577 1101 82-83 1.291225925343479 1101 84-85 1.1596326608017087 1101 86-87 1.0267493226743056 1101 88-89 1.0137759725376583 1101 90-91 0.5628695154498509 1101 92-93 0.8196854990188776 1101 94-95 0.6919583475708322 1101 96-97 0.3125994647607868 1101 98-99 0.4959810432262195 1101 100-101 0.5231924933148413 1102 1 -0.023187148766950827 1102 2 -0.06567260975926814 1102 3 -0.04592555289886491 1102 4 0.0680333143658558 1102 5 -0.08489636601261452 1102 6 0.023772684279670386 1102 7 -0.15720907739969192 1102 8 -0.25510022998525983 1102 9 -0.17486928753811526 1102 10-11 -0.013083427612976095 1102 12-13 -0.10228305849224739 1102 14-15 -0.20617737853031315 1102 16-17 0.10493307386312267 1102 18-19 0.06255072468999856 1102 20-21 -0.08702829786138011 1102 22-23 0.11542996084985901 1102 24-25 -0.003944866666756752 1102 26-27 -0.49056155352732844 1102 28-29 -0.26297219671388916 1102 30-31 -0.31499415361083294 1102 32-33 -0.25716361296889545 1102 34-35 -0.07498838818882092 1102 36-37 0.008768328576209683 1102 38-39 0.1009494295047304 1102 40-41 0.013927218049623491 1102 42-43 -0.2864967393936908 1102 44-45 0.3475990049781643 1102 46-47 0.4480319406521929 1102 48-49 0.006828908335776873 1102 50-51 0.32060729788400977 1102 52-53 0.5963998375041584 1102 54-55 0.5480451960578492 1102 56-57 0.7731764122729032 1102 58-59 0.6637746990371767 1102 60-61 0.5440562112536185 1102 62-63 1.031155469798854 1102 64-65 0.7418812205412806 1102 66-67 0.4631861700550033 1102 68-69 0.44804614482331573 1102 70-71 0.27835389523295717 1102 72-73 0.27022021713241884 1102 74-75 0.4818495783729375 1102 76-77 0.3614528384485105 1102 78-79 0.8179933992022477 1102 80-81 0.6822481501577222 1102 82-83 0.4992455386545025 1102 84-85 0.39433009526908336 1102 86-87 0.6035991088799229 1102 88-89 0.3328408544859762 1102 90-91 0.2745724342079825 1102 92-93 -0.02631282780767208 1102 94-95 0.3643765479798269 1102 96-97 0.28485267167657113 1102 98-99 0.2677322981063064 1102 100-101 0.8913138913442147 1103 1 -0.20965303461934326 1103 2 0.16685202307211 1103 3 -0.002097703856293265 1103 4 0.15977899200110812 1103 5 0.145350891842007 1103 6 0.15339714979371166 1103 7 0.10585097093643014 1103 8 -0.06405635565917578 1103 9 0.06355792941671012 1103 10-11 -0.0613498340082117 1103 12-13 0.2558341566034983 1103 14-15 0.19111998157379162 1103 16-17 0.1763682848688788 1103 18-19 -0.029311145546103035 1103 20-21 -0.00910953972790196 1103 22-23 0.16010601587867512 1103 24-25 0.281447399553727 1103 26-27 -0.1052668890915669 1103 28-29 0.3098921832823933 1103 30-31 0.17081872982475232 1103 32-33 0.18059201075298859 1103 34-35 0.2898099014560884 1103 36-37 0.1854159396563304 1103 38-39 0.10513236313551744 1103 40-41 0.1876235370680277 1103 42-43 0.49414324945181676 1103 44-45 0.49245632046432064 1103 46-47 0.8738429650061619 1103 48-49 0.8498991816207777 1103 50-51 1.0726174298788038 1103 52-53 1.031262265464754 1103 54-55 0.9678839207278642 1103 56-57 1.0940306602743917 1103 58-59 1.413379644194265 1103 60-61 1.2662397026088854 1103 62-63 1.4634965183208877 1103 64-65 1.0445280879887662 1103 66-67 1.5913466087982613 1103 68-69 1.9326738637301766 1103 70-71 1.9438934936713288 1103 72-73 2.0357551678667534 1103 74-75 2.2829046071887014 1103 76-77 1.8286865250073525 1103 78-79 2.358765382842332 1103 80-81 2.5228086632642466 1103 82-83 2.070367029637957 1103 84-85 1.475583581047104 1103 86-87 1.9577309177663302 1103 88-89 1.7484385492247725 1103 90-91 1.8435443620756153 1103 92-93 2.351894087975932 1103 94-95 2.1919583475708286 1103 96-97 2.401556519975511 1103 98-99 2.549662024821309 1103 100-101 2.9357691804314037 1104 1 -0.14518296903863614 1104 2 -0.08108535793063965 1104 3 -0.24289524986856037 1104 4 -0.03854975773445801 1104 5 -0.33280650185378846 1104 6 -0.08803504821771213 1104 7 -0.2172926719660424 1104 8 -0.25836564273343043 1104 9 -0.5463426417700887 1104 10-11 -0.2453195822629226 1104 12-13 -0.48387919224355613 1104 14-15 -0.5611277442565452 1104 16-17 -0.14376076103761193 1104 18-19 -0.38389650623999216 1104 20-21 -0.47835536160223313 1104 22-23 -0.19484955848138696 1104 24-25 -0.6621214601881888 1104 26-27 -0.4481111878011035 1104 28-29 -0.7391346836522388 1104 30-31 -0.7740458777487689 1104 32-33 -0.7348934980263664 1104 34-35 -0.7974283045942556 1104 36-37 -0.7015895606609917 1104 38-39 -0.40375276485263356 1104 40-41 -0.1434003681572733 1104 42-43 -0.41835410616485547 1104 44-45 -0.2502980692120147 1104 46-47 -0.7717303372997364 1104 48-49 0.031515428711948346 1104 50-51 0.250531540308252 1104 52-53 0.30381885526800545 1104 54-55 -0.5362677610999427 1104 56-57 0.009331062220660158 1104 58-59 0.37485097907897114 1104 60-61 0.5158430451094205 1104 62-63 0.5141100152534008 1104 64-65 0.8272391097784819 1104 66-67 0.8306104124792455 1104 68-69 0.902395674603877 1104 70-71 1.2189886914711998 1104 72-73 0.9685613874563472 1104 74-75 1.1549164540260186 1104 76-77 1.1051832459720252 1104 78-79 0.627619835983861 1104 80-81 0.9459628837000373 1104 82-83 0.8784905752271257 1104 84-85 0.9004037629806803 1104 86-87 0.7052187536030132 1104 88-89 -0.1547505770709705 1104 90-91 -0.144053480107587 1104 92-93 -0.02043508486096357 1104 94-95 0.2515891916579882 1104 96-97 0.562282138761212 1104 98-99 0.6352871570404766 1104 100-101 0.30053541694504915 1105 1 0.34044921486940893 1105 2 0.32461919719920473 1105 3 0.38364796000798407 1105 4 0.32224206857461013 1105 5 0.2579768101938953 1105 6 0.4886997325400557 1105 7 0.43426118073723785 1105 8 0.23269438280935617 1105 9 -0.07890969157851657 1105 10-11 0.0849805454509962 1105 12-13 -0.08397497768416429 1105 14-15 0.10814641496013877 1105 16-17 0.7505559694860153 1105 18-19 0.6550030254756294 1105 20-21 0.3581176056178563 1105 22-23 0.24092098217420954 1105 24-25 0.39013481907959147 1105 26-27 0.3764418134760348 1105 28-29 0.579830833589142 1105 30-31 0.28454288342620515 1105 32-33 0.10780552283357281 1105 34-35 0.3652473020468605 1105 36-37 0.0707071613483734 1105 38-39 0.3063366348919345 1105 40-41 0.7292049958274021 1105 42-43 1.094044786981165 1105 44-45 1.0992123607581874 1105 46-47 0.7201279002481513 1105 48-49 1.254163364003567 1105 50-51 1.8492969724070178 1105 52-53 2.3518712179755425 1105 54-55 2.363210740390059 1105 56-57 2.4329687803986033 1105 58-59 2.6566198168822908 1105 60-61 2.7810090732064765 1105 62-63 2.4492952004385913 1105 64-65 2.801855968016028 1105 66-67 2.8491289309977645 1105 68-69 2.1896987823092893 1105 70-71 1.6452871163328489 1105 72-73 1.7537359298148019 1105 74-75 2.425031396554761 1105 76-77 1.903820964149972 1105 78-79 2.5270983374738556 1105 80-81 2.143387319630225 1105 82-83 1.9987545173301484 1105 84-85 1.5896864364587557 1105 86-87 0.884603598217744 1105 88-89 0.3042213258664468 1105 90-91 0.7089158685514185 1105 92-93 0.5981260049644952 1105 94-95 0.607432103535384 1105 96-97 0.8120692822265134 1105 98-99 0.5960151263891369 1105 100-101 0.008176966540620612 1106 1 0.34945822387842185 1106 2 0.3788400847534277 1106 3 0.4800777231044151 1106 4 0.08400383033637127 1106 5 0.04860076748451547 1106 6 0.050594961101950275 1106 7 0.21420779401718448 1106 8 0.1325942827092561 1106 9 0.5352044225355996 1106 10-11 0.3277232881937451 1106 12-13 0.14950850579931796 1106 14-15 0.5112161513632145 1106 16-17 0.44850391743396045 1106 18-19 0.17936071649998553 1106 20-21 0.43252534669226606 1106 22-23 0.4126760705959711 1106 24-25 0.24165300393110556 1106 26-27 0.6619773490115648 1106 28-29 0.6676686714269806 1106 30-31 0.9502085490918759 1106 32-33 0.7287598104545339 1106 34-35 0.7851672219667876 1106 36-37 0.9844542417621227 1106 38-39 0.9627430412983387 1106 40-41 1.2419677585901638 1106 42-43 1.2695536291566754 1106 44-45 1.4750048198839778 1106 46-47 1.6067645368847892 1106 48-49 0.8118043383112052 1106 50-51 0.8451261349028485 1106 52-53 0.65342276952709 1106 54-55 0.5871012976139482 1106 56-57 0.30767682177332034 1106 58-59 0.20441761468009645 1106 60-61 0.2654935576909665 1106 62-63 0.5749208260642078 1106 64-65 0.6682223343823921 1106 66-67 0.9927725746414069 1106 68-69 0.7067992160763872 1106 70-71 0.993802298181361 1106 72-73 1.0471960566082572 1106 74-75 1.3664728379961986 1106 76-77 1.2515019784976538 1106 78-79 1.055042948751801 1106 80-81 1.4485257914353618 1106 82-83 1.6513237532327167 1106 84-85 1.8692993827383688 1106 86-87 1.7773296576104727 1106 88-89 1.9751422467873674 1106 90-91 1.8155224751580228 1106 92-93 2.4893505628557193 1106 94-95 2.4662909623942433 1106 96-97 2.7228133596372572 1106 98-99 2.6350541654281763 1106 100-101 3.097599722630047 1107 1 -0.21810934368915014 1107 2 -0.0941328882195478 1107 3 0.11521285823954486 1107 4 0.2056254519579923 1107 5 0.2377899566737085 1107 6 -0.03048611997913042 1107 7 0.09258617239555633 1107 8 -0.07010841999345274 1107 9 -0.14047125314007758 1107 10-11 -0.29389833342787597 1107 12-13 0.06842742471823726 1107 14-15 0.17337831352537592 1107 16-17 0.28634175527179906 1107 18-19 -0.19226090512163552 1107 20-21 -0.567474653307734 1107 22-23 -0.10759419967430262 1107 24-25 -0.6502388879607821 1107 26-27 -1.0745091374749194 1107 28-29 -0.9134124096541001 1107 30-31 -0.7119536130702926 1107 32-33 -0.6496185679238451 1107 34-35 -0.07969764289807557 1107 36-37 -0.279059271751386 1107 38-39 -0.246716418161121 1107 40-41 0.012238028860430461 1107 42-43 0.3236076832107315 1107 44-45 1.0020318469110094 1107 46-47 1.1473050774253295 1107 48-49 1.3050475815544544 1107 50-51 0.8924234322001467 1107 52-53 1.0115308776352023 1107 54-55 0.9925067030193517 1107 56-57 0.9022714163679026 1107 58-59 1.0895527498152262 1107 60-61 0.8803584225558296 1107 62-63 0.8924883936317798 1107 64-65 0.7290331451932133 1107 66-67 -0.20317337130453694 1107 68-69 0.6730154322926047 1107 70-71 1.0681266225056838 1107 72-73 1.4998987593109625 1107 74-75 0.9475539190772793 1107 76-77 1.1839344109300818 1107 78-79 1.6563943001031483 1107 80-81 2.0431203860299583 1107 82-83 2.03645888836785 1107 84-85 1.76119127463026 1107 86-87 1.3178701981510095 1107 88-89 1.718385490030613 1107 90-91 1.362819772455321 1107 92-93 1.5907019142070702 1107 94-95 1.5203450164482994 1107 96-97 2.2836241704480713 1107 98-99 2.7431622735362815 1107 100-101 2.665167290197612 1108 1 -0.11726222791666174 1108 2 0.23543548612494192 1108 3 0.3429256456538283 1108 4 0.16911879604835178 1108 5 0.24323570092542468 1108 6 -0.12084513571128497 1108 7 0.022799968280999394 1108 8 -0.13767598756101762 1108 9 -0.020866573833906443 1108 10-11 0.13611376822601784 1108 12-13 -0.47866817834751885 1108 14-15 -0.5089532717912846 1108 16-17 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0.08229183343522095 2316 64-65 0.2846842508443075 2316 66-67 0.6457619276307582 2316 68-69 0.04312190239907565 2316 70-71 0.609035713414773 2316 72-73 0.3410535504657517 2316 74-75 7.889723123319925E-4 2316 76-77 0.029634656630328493 2316 78-79 0.561554005262856 2316 80-81 0.7830057259152987 2316 82-83 0.7443212962302574 2316 84-85 0.645466458905446 2316 86-87 0.8528415331223442 2316 88-89 0.6661741878193084 2316 90-91 0.5957845554201029 2316 92-93 0.8502023237074816 2316 94-95 0.6427856388889204 2316 96-97 1.1560647928886922 2316 98-99 0.8253080556820649 2316 100-101 -1.182170957140638 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 7.0 7 14.0 8 31.0 9 64.0 10 111.0 11 242.0 12 361.0 13 549.0 14 696.0 15 1073.0 16 1594.0 17 2303.0 18 2148.0 19 1213.0 20 944.0 21 837.0 22 851.0 23 908.0 24 1003.0 25 1105.0 26 1216.0 27 1424.0 28 1595.0 29 1971.0 30 2385.0 31 2853.0 32 3849.0 33 5149.0 34 7180.0 35 10949.0 36 17449.0 37 27570.0 38 23263.0 39 2114.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.32212746211818 20.366577595724593 15.671152215306336 19.64014272685089 2 15.132826186076647 22.683240942941936 40.45259293033524 21.731339940646173 3 19.622916200044795 27.670943589415415 31.363389114644995 21.342751095894794 4 12.407416294732126 18.520740349698453 38.52042040600854 30.551422949560873 5 12.640883094028716 39.530456345238576 33.145622525296965 14.683038035435747 6 28.553146221095314 38.58570023115747 17.837517896707006 15.023635651040209 7 25.240155811330716 36.886812826439936 21.18810138934436 16.68492997288499 8 26.05061509174385 34.61790724832429 19.6021500215962 19.729327638335654 9 27.601321356870333 17.124849027778886 21.24009182310455 34.03373779224623 10-11 23.58725994417027 28.713116786511282 26.775873239323968 20.92375002999448 12-13 26.232373243323227 26.744279052654313 27.518936515681112 19.504411188341344 14-15 23.275317341609146 26.713884645225278 28.242003471361272 21.768794541804308 16-17 19.990721707205875 30.575574094366637 28.253601337353928 21.180102861073564 18-19 19.88041913293873 27.92753159494481 33.191489361702125 19.00055991041433 20-21 21.034129720131496 26.969437623477283 33.11870615806692 18.877726498324307 22-23 20.670996536637258 27.08581620981739 32.77996848579861 19.463218767746735 24-25 20.317792966640937 27.020001039846104 33.37985978075245 19.282346212760512 26-27 20.413942048113263 27.223788669586256 33.18335433039374 19.178914951906734 28-29 20.029676557519327 27.09565690379916 33.97405900915494 18.900607529526575 30-31 20.20530135134054 27.77768887964348 33.23345627945306 18.783553489562916 32-33 19.83515033233885 27.93086072162722 32.801564512129765 19.43242443390416 34-35 19.794991161343475 27.601804525712094 33.08257144913973 19.520632863804703 36-37 20.752286899376426 27.746779141717763 32.48377071408858 19.01716324481723 38-39 20.578812857708506 27.434017653952143 32.870986965512 19.11618252282735 40-41 19.729942684814475 28.13363677451094 33.11641822086945 19.020002319805137 42-43 20.080634516852854 28.574056964126438 32.357399696820615 18.98790882220009 44-45 20.433930571108622 28.38585826267797 31.6649336106223 19.51527755559111 46-47 20.41666000095986 28.950231166711994 30.544801548577006 20.08830728375114 48-49 20.528079731880755 29.118374007150916 30.34178804821667 20.011758212751662 50-51 21.347299042956955 29.187213297019287 29.794313732547863 19.671173927475895 52-53 20.59568137005915 29.57169653605724 29.902852823427306 19.929769270456298 54-55 20.417141600175974 29.62545243665887 29.853020577119203 20.104385386045955 56-57 20.520706272870886 30.15257253694335 29.354316223079845 19.972404967105923 58-59 20.528951136209116 29.221490789547357 29.688612313131397 20.560945761112134 60-61 20.25827247786407 29.42338609695816 29.659342680946704 20.65899874423106 62-63 20.894555401806066 29.121841581149067 29.80331618982107 20.18028682722379 64-65 20.679545636349093 28.983681305495562 30.328373730101593 20.008399328053756 66-67 20.187727710833737 29.31215830877089 30.104821970621053 20.39529200977432 68-69 20.30738835875573 29.479447452827923 29.943769446732947 20.269394741683396 70-71 20.00407952677489 29.630162901103475 29.548172412000206 20.81758516012143 72-73 20.07031549980801 28.966146166645334 29.44331562779982 21.520222705746832 74-75 20.433621027540255 28.803682699152915 29.568778646104132 21.193917627202698 76-77 20.22348334873081 28.724889118184617 29.65353681996153 21.398090713123047 78-79 20.11799056075514 28.18334533237341 29.736421086313097 21.962243020558354 80-81 20.428641130032073 28.94027498940195 29.166633339465537 21.464450541100437 82-83 19.93392819290408 29.638724798125033 28.860821258163988 21.5665257508069 84-85 19.49600265556449 29.097228054599043 28.54731823980867 22.859451050027797 86-87 19.62078977468146 29.02865872679427 28.242803324188348 23.10774817433592 88-89 19.969524997900344 28.10019156858275 29.076831399650455 22.853452033866446 90-91 19.40130776460237 27.865384230838448 29.865024295626785 22.868283708932392 92-93 19.327321082205202 28.342498350296946 28.931592313383593 23.39858825411426 94-95 18.96491045647601 29.454580357214272 28.53674923814018 23.043759948169537 96-97 18.982583135035693 30.167369873422782 28.08414485392629 22.765902137615228 98-99 19.144307179165185 30.540347060305628 26.772995044852287 23.542350715676903 100-101 19.321953911742828 31.138768686860608 25.78286847809568 23.75640892330089 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 263.0 1 190.0 2 114.0 3 108.5 4 101.5 5 118.5 6 169.5 7 276.0 8 423.0 9 507.0 10 476.5 11 426.0 12 409.0 13 405.0 14 431.0 15 444.5 16 432.5 17 449.0 18 491.0 19 503.0 20 534.0 21 588.5 22 639.5 23 703.5 24 810.0 25 975.5 26 1144.0 27 1303.0 28 1460.0 29 1703.0 30 2023.5 31 2299.5 32 2511.5 33 2674.5 34 2929.0 35 3263.0 36 3642.0 37 3898.0 38 3932.5 39 3947.5 40 3948.0 41 3980.5 42 3907.0 43 3914.0 44 4047.0 45 4082.0 46 4213.0 47 4443.5 48 5693.0 49 5549.5 50 4141.0 51 3739.5 52 3404.0 53 3253.5 54 3028.0 55 2726.5 56 2364.0 57 2092.0 58 1886.0 59 1660.5 60 1365.0 61 1053.0 62 829.0 63 615.0 64 433.0 65 291.5 66 214.5 67 162.5 68 122.5 69 87.5 70 62.5 71 44.5 72 26.5 73 18.5 74 13.0 75 8.0 76 4.5 77 3.5 78 2.5 79 0.5 80 0.0 81 1.0 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023195731985314702 2 0.007998528270798173 3 0.00879838109787799 4 7.998528270798173E-4 5 0.006398822616638538 6 0.0 7 0.0 8 7.998528270798173E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.002399558481239452 20-21 0.0 22-23 0.0 24-25 0.0035993377218591777 26-27 0.004399190548938995 28-29 0.006798749030178447 30-31 0.02879470177487342 32-33 0.0 34-35 0.003199411308319269 36-37 0.013997424473896803 38-39 0.006798749030178447 40-41 0.009998160338497717 42-43 0.011597865992657351 44-45 0.002399558481239452 46-47 0.003999264135399087 48-49 0.003199411308319269 50-51 0.0019996320676995433 52-53 0.005199043376018812 54-55 0.004399190548938995 56-57 3.9992641353990864E-4 58-59 0.0015997056541596345 60-61 0.0 62-63 0.0 64-65 0.010398086752037624 66-67 0.001199779240619726 68-69 0.0015997056541596345 70-71 0.006798749030178447 72-73 0.011997792406197258 74-75 0.004799116962478904 76-77 0.0019996320676995433 78-79 0.010398086752037624 80-81 0.0 82-83 0.005998896203098629 84-85 0.0019996320676995433 86-87 0.0 88-89 0.0019996320676995433 90-91 3.9992641353990864E-4 92-93 3.9992641353990864E-4 94-95 0.0 96-97 3.9992641353990864E-4 98-99 0.001199779240619726 100-101 0.0015997056541596345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 125023.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.34293689961046 #Duplication Level Percentage of deduplicated Percentage of total 1 90.42634506773112 68.12986410500469 2 6.048027517092021 9.113523111747439 3 1.4894475349271732 3.366580549178951 4 0.6656333602276105 2.0060308903161816 5 0.3354707206250796 1.2637674667861114 6 0.22930910017410505 1.0366092638954434 7 0.12845556074567924 0.6774753445366053 8 0.12420909592764023 0.7486622461467091 9 0.09342222599685761 0.6334834390472153 >10 0.41403031975880084 5.395007318653368 >50 0.023355556499214402 1.2277740895675195 >100 0.018047475476665676 3.1994113083192692 >500 0.0021232324090194915 1.0933988146181102 >1k 0.0021232324090194915 2.1084120521823984 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1447 1.1573870407844955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1189 0.9510250113979027 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 797 0.6374827031826144 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 570 0.4559161114354959 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 455 0.3639330363213169 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 441 0.35273509674219944 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 378 0.3023443686361709 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 339 0.2711501083800581 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 299 0.23915599529686538 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 294 0.2351567311614663 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.22155923310110942 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 249 0.19916335394287452 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.1767674747846396 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 183 0.14637306735560657 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 152 0.12157762971613223 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 140 0.11197939579117443 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 126 0.10078145621205697 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.998528270798173E-4 0.0 10-11 0.0 0.0 0.0 7.998528270798173E-4 0.0 12-13 0.0 0.0 0.0 7.998528270798173E-4 0.0 14-15 0.0 0.0 0.0 7.998528270798173E-4 0.0 16-17 0.0 0.0 0.0 0.0015997056541596345 0.0 18-19 0.0 0.0 0.0 0.0015997056541596345 0.0 20-21 0.0 0.0 0.0 0.0019996320676995433 0.0 22-23 0.0 0.0 0.0 0.004399190548938996 0.0 24-25 0.0 0.0 0.0 0.006398822616638538 0.0 26-27 0.0 0.0 0.0 0.009598233924957808 0.0 28-29 0.0 0.0 0.0 0.015197203714516529 0.0 30-31 0.0 0.0 0.0 0.028394775361333516 0.0 32-33 0.0 0.0 0.0 0.043591979075850044 0.0 34-35 0.0 0.0 0.0 0.053190213000807854 0.0 36-37 0.0 0.0 0.0 0.06598785823408493 0.0 38-39 0.0 0.0 0.0 0.08118506194860145 0.0 40-41 0.0 0.0 0.0 0.10038152979851707 0.0 42-43 0.0 0.0 0.0 0.157571006934724 0.0 44-45 0.0 0.0 0.0 0.22355886516880893 0.0 46-47 0.0 0.0 0.0 0.2835478271997952 0.0 48-49 0.0 0.0 0.0 0.3527350967421994 0.0 50-51 0.0 0.0 0.0 0.42112251345752383 0.0 52-53 0.0 0.0 0.0 0.5830927109411868 0.0 54-55 0.0 0.0 0.0 0.7026707085896196 0.0 56-57 0.0 0.0 0.0 0.9498252321572831 0.0 58-59 0.0 0.0 0.0 1.299760844004703 0.0 60-61 0.0 0.0 0.0 1.6173024163553906 0.0 62-63 0.0 0.0 0.0 2.175999616070643 0.0 64-65 0.0 0.0 0.0 2.4639466338193774 0.0 66-67 0.0 0.0 0.0 2.987050382729578 0.0 68-69 0.0 0.0 0.0 3.5981379426185582 0.0 70-71 0.0 0.0 0.0 4.074450301144589 0.0 72-73 0.0 0.0 0.0 5.016277005031075 0.0 74-75 0.0 0.0 0.0 5.807331451013013 0.0 76-77 0.0 0.0 0.0 6.26004815114019 0.0 78-79 0.0 0.0 0.0 6.728361981395423 0.0 80-81 0.0 0.0 0.0 7.701782871951561 0.0 82-83 0.0 0.0 0.0 8.18489397950777 0.0 84-85 0.0 0.0 0.0 9.117922302296378 0.0 86-87 0.0 0.0 0.0 11.146349071770794 0.0 88-89 0.0 0.0 0.0 13.450325140174208 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGACAT 25 0.005013096 56.993515 7 GGGATAG 25 0.005013096 56.993515 7 GTTGGAA 35 2.779878E-4 54.27954 8 TATTCGT 40 5.372669E-4 47.4946 4 GTAAGGT 40 5.372669E-4 47.4946 4 ATATTCG 40 5.372669E-4 47.4946 3 TCGTAGC 45 9.597503E-4 42.217422 7 GTAGCTT 75 6.8783083E-6 37.99568 9 CGTAGCT 50 0.001611211 37.995678 8 TGGACAG 55 0.0025723102 34.541527 5 TTCGTAG 55 0.0025723102 34.541527 6 AATGCGT 35 2.0501245E-4 33.924713 84-85 ATGGGAC 150 9.385985E-10 31.663067 5 GGTGTGC 60 0.0039398037 31.663067 8 AAGGTGT 60 0.0039398037 31.663067 6 ATGCGTA 30 0.0039472883 31.663067 84-85 TGCGTAG 30 0.0039472883 31.663067 86-87 TAAGGTG 65 0.005827664 29.227448 5 TTTCGTC 35 0.008367534 27.15063 64-65 CTACTCC 70 0.00836813 27.13977 4 >>END_MODULE