FastQCFastQC Report
Thu 26 May 2016
SRR936925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129457
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15481.1957638443653105No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.9114995712862186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7930.612558610194891No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4550.35146805502985545No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4000.308982905520752No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3590.277312157704875No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3540.2734498713858656No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2640.20392871764369636No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2480.1915694014228663No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2460.19002448689526252No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.18461728604864935No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2340.18075499972963996No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2210.17071305530021552No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.13826985022053656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTAGT250.004997126357.04062
AGTACAC500.001604892438.0270652
TCTAGGA1106.8876034E-834.570062
GTCCAGG753.0576345E-431.6769751
GTATCAA13600.030.395921
GGTATCA9500.029.0094411
AAGTATC700.00833553327.1621917
CCGAAGG350.00837436627.14644664-65
TATCAAC16350.025.2932342
CTAGGAC950.001215617925.017813
ATCAACG16550.024.9875763
TCAACGC16650.024.8375024
GAGTACT8700.024.57696312-13
CAACGCA16950.024.3978985
CATGGGA5100.024.2329354
AACGCAG17400.024.04016
TGGGAAC1201.6912183E-423.7669186
TACATGG8000.023.7669162
ACATGGG7600.023.1414743
GTACATG8150.022.7374611
Produced by FastQC (version 0.11.5)