##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 129457 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.002966235893 33.0 31.0 34.0 30.0 34.0 2 32.106490958387724 33.0 31.0 34.0 30.0 34.0 3 32.07431038877774 34.0 31.0 34.0 30.0 34.0 4 35.63827371250686 37.0 35.0 37.0 35.0 37.0 5 35.63443459990576 37.0 35.0 37.0 35.0 37.0 6 35.75843716446388 37.0 35.0 37.0 35.0 37.0 7 35.71885645426667 37.0 35.0 37.0 35.0 37.0 8 35.68235784855203 37.0 35.0 37.0 35.0 37.0 9 37.33262782236573 39.0 37.0 39.0 35.0 39.0 10-11 37.22913399816155 39.0 37.0 39.0 34.0 39.0 12-13 37.140228029384275 39.0 37.0 39.0 34.0 39.0 14-15 38.30074464880231 40.0 38.0 41.0 34.0 41.0 16-17 38.26171624554871 40.0 38.0 41.0 34.0 41.0 18-19 38.28749314444178 40.0 38.0 41.0 34.0 41.0 20-21 38.24140834408336 40.0 38.0 41.0 34.0 41.0 22-23 38.09993279621805 40.0 38.0 41.0 34.0 41.0 24-25 38.193098866805194 40.0 38.0 41.0 34.0 41.0 26-27 38.11605784159991 40.0 38.0 41.0 34.0 41.0 28-29 37.980097638598146 40.0 38.0 41.0 33.5 41.0 30-31 37.72665054805843 40.0 37.0 41.0 33.0 41.0 32-33 37.4260217678457 40.0 36.5 41.0 33.0 41.0 34-35 37.18583390623914 40.0 36.0 41.0 32.5 41.0 36-37 36.95824868489151 39.5 35.5 41.0 31.5 41.0 38-39 36.756208625257806 39.0 35.0 41.0 31.0 41.0 40-41 36.49317920236063 39.0 35.0 41.0 30.5 41.0 42-43 36.18567941478638 39.0 35.0 41.0 30.0 41.0 44-45 35.776937515931934 39.0 35.0 41.0 29.0 41.0 46-47 35.22143259924145 39.0 35.0 40.0 26.0 41.0 48-49 34.79338699336459 39.0 35.0 40.0 23.5 41.0 50-51 34.00932742146041 38.0 34.0 40.0 22.0 40.5 52-53 34.13253821732313 38.5 34.5 40.0 21.5 40.5 54-55 34.47117961948756 39.0 35.0 40.5 20.5 41.0 56-57 34.34531157063735 39.0 35.0 41.0 18.5 41.0 58-59 34.085704133418815 38.5 34.5 41.0 14.0 41.0 60-61 33.810635964065284 38.0 34.0 40.0 10.5 41.0 62-63 33.438350185775974 38.0 34.0 40.0 7.5 41.0 64-65 33.05808106166526 37.5 33.5 40.0 2.0 41.0 66-67 32.668963439597704 37.0 33.0 40.0 2.0 41.0 68-69 32.23898669056135 36.0 33.0 39.0 2.0 41.0 70-71 31.790579111210672 36.0 32.0 39.0 2.0 41.0 72-73 31.34088925280209 35.0 31.5 39.0 2.0 40.0 74-75 30.89559081393822 35.0 31.0 38.0 2.0 40.0 76-77 30.44008049004689 35.0 31.0 37.0 2.0 39.0 78-79 29.98992715728002 35.0 30.5 37.0 2.0 39.0 80-81 29.57453826367056 35.0 30.0 36.0 2.0 39.0 82-83 29.132159713263864 34.5 29.5 36.0 2.0 37.5 84-85 28.74348239183667 34.0 29.0 35.0 2.0 37.0 86-87 28.358261816664992 34.0 29.0 35.0 2.0 36.5 88-89 27.96981623241694 34.0 28.5 35.0 2.0 36.0 90-91 27.536931181782368 34.0 27.0 35.0 2.0 36.0 92-93 27.208061364005037 34.0 26.0 35.0 2.0 35.5 94-95 26.826915500899915 34.0 25.5 35.0 2.0 35.0 96-97 26.348505681423177 34.0 25.0 35.0 2.0 35.0 98-99 25.718817831403477 34.0 21.5 35.0 2.0 35.0 100-101 23.45675784237237 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4736884961869947 1101 2 0.45104034697388684 1101 3 0.3590831466996782 1101 4 0.5864733663495088 1101 5 0.23525775584669617 1101 6 0.34576204022434354 1101 7 0.12810711566707056 1101 8 0.15486732849247886 1101 9 0.20680942247415146 1101 10-11 0.5606579305462134 1101 12-13 0.6095437941531898 1101 14-15 0.588426736441555 1101 16-17 0.6466447821651968 1101 18-19 0.8131143972056876 1101 20-21 0.6746960933227228 1101 22-23 0.5017670765205509 1101 24-25 0.5167753622260349 1101 26-27 0.6164445655424018 1101 28-29 0.7311430517585578 1101 30-31 0.6324487201474369 1101 32-33 0.7623903453821725 1101 34-35 0.9316827895250626 1101 36-37 0.6052208057300277 1101 38-39 0.3750100108677188 1101 40-41 0.47027905794377745 1101 42-43 0.5859520521640036 1101 44-45 0.9944912286389851 1101 46-47 1.2565952767778796 1101 48-49 1.029262177872944 1101 50-51 1.1321461824280519 1101 52-53 1.2550940684906706 1101 54-55 1.1831460887449268 1101 56-57 1.2191832882336868 1101 58-59 1.3066697793978435 1101 60-61 1.6402976895326447 1101 62-63 1.7109046867663906 1101 64-65 1.8449132480170043 1101 66-67 1.6839657884928272 1101 68-69 2.0110703734336646 1101 70-71 1.7609361692006118 1101 72-73 1.3139203329690332 1101 74-75 1.4781308584320314 1101 76-77 1.7798314713134928 1101 78-79 1.7375098057677185 1101 80-81 1.7123761561022057 1101 82-83 1.7172767840395515 1101 84-85 1.649663778178553 1101 86-87 1.456146309595134 1101 88-89 1.7834316165006179 1101 90-91 1.443619865364937 1101 92-93 1.485098130517354 1101 94-95 1.7233115141047932 1101 96-97 1.1976727467452655 1101 98-99 0.7809012564737294 1101 100-101 0.6442579167899112 1102 1 0.22159863202816865 1102 2 0.5341614284785869 1102 3 0.23840981859100552 1102 4 0.10686586896184025 1102 5 0.19447275062204028 1102 6 0.07078163270083593 1102 7 0.14350353677470196 1102 8 -0.06724604663813238 1102 9 0.17211441202482547 1102 10-11 0.2987146834197816 1102 12-13 0.22501695246040043 1102 14-15 0.3973021282910807 1102 16-17 0.2147286379645692 1102 18-19 0.2519450529005667 1102 20-21 0.34808134671874313 1102 22-23 0.48438691716841475 1102 24-25 0.06531572272759689 1102 26-27 0.5413808899938104 1102 28-29 0.1376624483102873 1102 30-31 0.3069033962185017 1102 32-33 0.4864303140342088 1102 34-35 0.8325448584905857 1102 36-37 1.0235691599620012 1102 38-39 0.9078927172418005 1102 40-41 1.1885719001590331 1102 42-43 1.604826137848434 1102 44-45 1.5062793686598752 1102 46-47 1.6498897516995115 1102 48-49 1.7858501480923792 1102 50-51 2.3731418067749743 1102 52-53 2.2523021405909844 1102 54-55 2.5622849994032393 1102 56-57 2.5226136043256417 1102 58-59 2.427383925165863 1102 60-61 2.3907630108492555 1102 62-63 3.1111953085009745 1102 64-65 2.658123475289731 1102 66-67 2.8906011071971136 1102 68-69 2.66401414302614 1102 70-71 2.767744554780549 1102 72-73 2.9741786271174107 1102 74-75 3.1902928882125963 1102 76-77 2.89101877538873 1102 78-79 2.6898919243675117 1102 80-81 3.082624980553618 1102 82-83 3.7747807025348514 1102 84-85 3.6017928272903568 1102 86-87 3.3229174694697434 1102 88-89 2.889524876688707 1102 90-91 2.9291622504224115 1102 92-93 2.7709033813010535 1102 94-95 3.07627814158651 1102 96-97 2.5882324384903015 1102 98-99 2.1715099294099858 1102 100-101 2.4764075380020287 1103 1 0.02646405645880634 1103 2 -0.05580363363321439 1103 3 0.35645459084772924 1103 4 0.4267739952558358 1103 5 0.40558817505267797 1103 6 0.3085533718312732 1103 7 -0.12723274686918984 1103 8 0.026827452326671164 1103 9 0.547470260886108 1103 10-11 0.2657339639580769 1103 12-13 0.5889047630194071 1103 14-15 0.6366266624525707 1103 16-17 0.26267118454842375 1103 18-19 0.2782616936873197 1103 20-21 0.23044094816802385 1103 22-23 -0.0019129016721706193 1103 24-25 -0.18943971205501242 1103 26-27 0.08364602974535984 1103 28-29 0.09685305390035381 1103 30-31 0.34200303389965825 1103 32-33 0.47070826434476487 1103 34-35 0.6019547963787844 1103 36-37 0.6599239736265972 1103 38-39 1.1065857814240019 1103 40-41 1.34779809063523 1103 42-43 1.2322847299809396 1103 44-45 1.166281956651595 1103 46-47 0.5952993013889554 1103 48-49 1.447971654303558 1103 50-51 1.1068536338971242 1103 52-53 1.408260991522667 1103 54-55 1.5417589900864712 1103 56-57 1.7464910629177766 1103 58-59 1.7799169220602735 1103 60-61 1.5624924363150967 1103 62-63 1.6248146149191953 1103 64-65 1.3237387703207908 1103 66-67 0.3621331982944156 1103 68-69 1.5773002455106173 1103 70-71 1.424091604469993 1103 72-73 1.0696593465791153 1103 74-75 1.1494834938026557 1103 76-77 1.384613495885624 1103 78-79 0.8020651904130567 1103 80-81 0.8503850737213199 1103 82-83 1.0595244913547255 1103 84-85 0.8299242972696561 1103 86-87 0.14900442599148178 1103 88-89 0.5904177338315648 1103 90-91 0.7467897090145392 1103 92-93 0.6508529154625435 1103 94-95 0.22749773268381546 1103 96-97 0.7005836289664913 1103 98-99 0.5261941944203414 1103 100-101 0.7975611353105201 1104 1 0.4065579816216598 1104 2 0.5213311439257389 1104 3 0.7029677454202705 1104 4 0.5998790803439533 1104 5 0.5742999863943936 1104 6 0.6203243156276628 1104 7 0.6240675611649422 1104 8 0.6233027338496697 1104 9 0.6491215258459633 1104 10-11 0.6209326712246579 1104 12-13 0.8333121760376372 1104 14-15 0.477332616095957 1104 16-17 0.27900709324912043 1104 18-19 0.6779486098111391 1104 20-21 0.7799283995642767 1104 22-23 1.0742624252984925 1104 24-25 0.8545687715080845 1104 26-27 0.9537919672295843 1104 28-29 0.7604393792045911 1104 30-31 0.9050995344298798 1104 32-33 0.6665624278553466 1104 34-35 0.5530733137751369 1104 36-37 0.8384827778481778 1104 38-39 1.1860939367539984 1104 40-41 0.6288666156061922 1104 42-43 0.23541556874273795 1104 44-45 -0.06257225735638627 1104 46-47 0.30594920291902383 1104 48-49 0.8569248432143297 1104 50-51 0.5474939409213064 1104 52-53 0.4714332381519597 1104 54-55 0.662069044118688 1104 56-57 0.44290069782157815 1104 58-59 0.8213752869544848 1104 60-61 0.9030216490606335 1104 62-63 1.100651609313985 1104 64-65 1.0193404468344518 1104 66-67 0.9779994811808521 1104 68-69 1.371775830018013 1104 70-71 1.1510016686016868 1104 72-73 1.1290947856540008 1104 74-75 1.27241941260284 1104 76-77 1.6402565802667795 1104 78-79 1.7192999528227944 1104 80-81 1.704195102504837 1104 82-83 2.106335580583629 1104 84-85 1.9000651850139363 1104 86-87 1.700966249957549 1104 88-89 1.6153886978269192 1104 90-91 0.9513294252191571 1104 92-93 1.223266186179103 1104 94-95 1.2661282432125276 1104 96-97 0.8718960563764817 1104 98-99 1.0968148074587702 1104 100-101 0.5175025583272337 1105 1 0.3065821303780041 1105 2 0.40622207204294014 1105 3 0.28487227733687703 1105 4 0.44540753562850455 1105 5 0.5371913974867226 1105 6 0.05500192973053686 1105 7 -0.02754638071704818 1105 8 -0.05342591462493118 1105 9 0.08790442852647828 1105 10-11 0.16291125932736605 1105 12-13 0.219370294044559 1105 14-15 0.33449272235048255 1105 16-17 0.27011230133091146 1105 18-19 0.5343037575210303 1105 20-21 0.33784516685076227 1105 22-23 0.5176738396106515 1105 24-25 0.1189976534206707 1105 26-27 -0.1514563624814329 1105 28-29 0.15893410672612873 1105 30-31 0.07015628400727536 1105 32-33 -0.010166220619254318 1105 34-35 0.004574561460884752 1105 36-37 0.33312980352635435 1105 38-39 0.2803143344035206 1105 40-41 0.9659027582448445 1105 42-43 0.9837452797626227 1105 44-45 0.9779171574387533 1105 46-47 0.9029064596203042 1105 48-49 0.9195908659141594 1105 50-51 1.140184917336022 1105 52-53 1.1636573386107898 1105 54-55 1.522448984551751 1105 56-57 2.130415790794281 1105 58-59 2.25202810833418 1105 60-61 2.016923283126488 1105 62-63 2.231296381108244 1105 64-65 2.329611717863987 1105 66-67 1.731360183104698 1105 68-69 1.819413276689506 1105 70-71 1.2663522280478752 1105 72-73 1.2615909621009145 1105 74-75 1.3501784080145782 1105 76-77 1.3944222407352669 1105 78-79 1.7291606949945724 1105 80-81 1.7316039409496575 1105 82-83 1.4007089203566316 1105 84-85 1.2451509130989393 1105 86-87 1.4219273704598407 1105 88-89 1.5748590351045486 1105 90-91 1.432694634910856 1105 92-93 1.165550658528776 1105 94-95 1.7056356085832078 1105 96-97 1.922648692615713 1105 98-99 1.9918482132383701 1105 100-101 1.499793470345267 1106 1 -0.7351524228241537 1106 2 -0.2666692677239446 1106 3 -0.1129219324638484 1106 4 -0.16218740108035945 1106 5 0.18827549323807347 1106 6 0.0862088478907097 1106 7 0.046523051542635585 1106 8 -0.38554773440184675 1106 9 -0.06437029261652327 1106 10-11 -0.2400841035000596 1106 12-13 -0.32512149690668224 1106 14-15 -0.3056250868987931 1106 16-17 -0.04753402026327791 1106 18-19 -0.18934582262685495 1106 20-21 0.2444750703474412 1106 22-23 -0.01979952797926643 1106 24-25 0.3598173050060822 1106 26-27 0.013789908981152621 1106 28-29 -0.05606777320870293 1106 30-31 -0.274122447663359 1106 32-33 -0.5764652555860437 1106 34-35 -0.6278981794840988 1106 36-37 -0.5739387514304042 1106 38-39 -1.0033678312814018 1106 40-41 0.19318687906199017 1106 42-43 0.3134231842619286 1106 44-45 -0.8514474245890682 1106 46-47 -0.19929933057897387 1106 48-49 -0.628410136717747 1106 50-51 -0.557270904195498 1106 52-53 -0.8978165302950885 1106 54-55 -0.9544278170524549 1106 56-57 -0.6092758998937668 1106 58-59 -0.29265167609995757 1106 60-61 -0.15383746383429298 1106 62-63 -0.45137061976906523 1106 64-65 -0.11135832850773753 1106 66-67 -0.2222680911151258 1106 68-69 -0.724138546847275 1106 70-71 -0.4337981667384412 1106 72-73 -0.7093327125450344 1106 74-75 -0.35153859912917795 1106 76-77 -0.17187679423152247 1106 78-79 -0.4770054912443058 1106 80-81 -0.675088627041319 1106 82-83 -0.4953933480980588 1106 84-85 -0.09098143431301864 1106 86-87 0.05076557073556387 1106 88-89 0.06238880073933828 1106 90-91 -0.45707851961978463 1106 92-93 -0.4812722516103385 1106 94-95 -0.0341187465991446 1106 96-97 0.5966053287856603 1106 98-99 -0.23487192713009364 1106 100-101 -1.5594244767659013 1107 1 0.1777389829053604 1107 2 0.2076811653206896 1107 3 0.5111620115734574 1107 4 0.39277595668113463 1107 5 0.5437052067623895 1107 6 0.32571584322715097 1107 7 0.1777688876518937 1107 8 0.21455219897590183 1107 9 1.0210727453581612 1107 10-11 0.3133802535952128 1107 12-13 0.29779655772355795 1107 14-15 0.6456573914489709 1107 16-17 0.47295232217509664 1107 18-19 0.6095382546549573 1107 20-21 0.5505210397012092 1107 22-23 0.5080985399754283 1107 24-25 0.21416440693811722 1107 26-27 0.4644894426253856 1107 28-29 0.6923499483102873 1107 30-31 0.7759275190255153 1107 32-33 0.5604434719289486 1107 34-35 0.20754485849058568 1107 36-37 0.6016941599620012 1107 38-39 0.454493594434787 1107 40-41 0.8919710229660538 1107 42-43 0.47872964662035855 1107 44-45 1.058362701993211 1107 46-47 1.0384588306468814 1107 48-49 1.3874126480923792 1107 50-51 1.667412640108303 1107 52-53 1.894571877433087 1107 54-55 2.066808025719034 1107 56-57 2.498901981518621 1107 58-59 1.3167753725342806 1107 60-61 1.3220952476913652 1107 62-63 1.4267106593781662 1107 64-65 1.4274491331844708 1107 66-67 1.7239344405304422 1107 68-69 1.8675503272366676 1107 70-71 1.8277774495173915 1107 72-73 1.2654341095735546 1107 74-75 1.6660330197915414 1107 76-77 1.4124003543361 1107 78-79 1.9778579331394397 1107 80-81 1.6722631384483542 1107 82-83 1.5330043867453789 1107 84-85 1.8463103711500075 1107 86-87 1.6650227326276372 1107 88-89 1.1806432977413372 1107 90-91 1.347884838141706 1107 92-93 1.3901468023536836 1107 94-95 1.891930553867212 1107 96-97 2.159230245507846 1107 98-99 1.0344485259012153 1107 100-101 1.8027507397564193 1108 1 0.4160430764726115 1108 2 0.09145309514525124 1108 3 0.2852848185910055 1108 4 0.3065186467396188 1108 5 0.3229449728442617 1108 6 0.3381427438119502 1108 7 0.8049618701080306 1108 8 0.5508095089174176 1108 9 0.590517189802604 1108 10-11 0.662429961197553 1108 12-13 -0.06404554753959957 1108 14-15 -0.27283676059780504 1108 16-17 0.44042308240901207 1108 18-19 0.6347575529005667 1108 20-21 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0.3815777483944629 2311 5 0.45355963005466293 2311 6 0.30947844121147483 2311 7 -0.16489628592033512 2311 8 0.454473811517893 2311 9 0.09022168152837651 2311 10-11 -0.07323788750220928 2311 12-13 0.018401260971039335 2311 14-15 1.0774882985038445 2311 16-17 0.8377339571135067 2311 18-19 -0.12632400738311844 2311 20-21 -0.12571076321033559 2311 22-23 -0.2868342707748539 2311 24-25 0.028414126982916343 2311 26-27 1.0216003048874214 2311 28-29 0.7322435653315651 2311 30-31 -0.34347337683114887 2311 32-33 0.1892228672256948 2311 34-35 0.5054171989161134 2311 36-37 0.8490345854939179 2311 38-39 0.7201710860361317 2311 40-41 0.6608679994498132 2311 42-43 1.02282259174914 2311 44-45 0.7950648296527802 2311 46-47 -0.10193870574729402 2311 48-49 0.5097530736242959 2311 50-51 0.3748594486189418 2311 52-53 0.27657075761226224 2311 54-55 0.26291664833940587 2311 56-57 0.3736220263114589 2311 58-59 0.594770893250967 2311 60-61 0.8849451917003179 2311 62-63 0.9563859113378612 2311 64-65 1.689207251885481 2311 66-67 0.7718067809559699 2311 68-69 1.2722255791963555 2311 70-71 1.1843004058443753 2311 72-73 1.5575673682521654 2311 74-75 1.8699803882125963 2311 76-77 2.1576411158142648 2311 78-79 2.024610452736301 2311 80-81 0.9366808316174478 2311 82-83 0.06533921317315006 2311 84-85 1.2635836074322029 2311 86-87 1.269725980108042 2311 88-89 0.882211046901471 2311 90-91 1.4483887575146106 2311 92-93 0.9746932749180743 2311 94-95 0.4563202515155851 2311 96-97 1.3434407718236336 2311 98-99 1.63383262444545 2311 100-101 2.194548052186427 2312 1 0.563797213588451 2312 2 0.17744600294667023 2312 3 -0.17836766367850032 2312 4 -0.08650735798851628 2312 5 -0.8655893061155524 2312 6 -0.37137262261830983 2312 7 0.11169945876051202 2312 8 -0.05616448635444726 2312 9 -0.6331825737907764 2312 10-11 -1.2647272492043342 2312 12-13 -0.3433008666885371 2312 14-15 -1.060809573836579 2312 16-17 -0.20481923437585436 2312 18-19 0.37367599261688156 2312 20-21 0.70407647083222 2312 22-23 0.2982721122038683 2312 24-25 0.592243914216958 2312 26-27 0.3833024325470049 2312 28-29 0.3492648419273081 2312 30-31 -0.21581380236305847 2312 32-33 0.14666967573633372 2312 34-35 0.6011618797671829 2312 36-37 0.3383962876215705 2312 38-39 0.9329370434829443 2312 40-41 0.4693786377476883 2312 42-43 0.9164396130257373 2312 44-45 0.8057031275251276 2312 46-47 0.6214655495718517 2312 48-49 0.4565615842625874 2312 50-51 -0.114502253508725 2312 52-53 -0.13832285940901556 2312 54-55 0.05015069089260038 2312 56-57 0.9055369199284726 2312 58-59 1.3607283400594739 2312 60-61 0.6402643406364916 2312 62-63 -0.04361408866214589 2312 64-65 -0.3746225353485677 2312 66-67 -1.8026613041504085 2312 68-69 -1.972455271867478 2312 70-71 -1.4220825728790274 2312 72-73 -0.9849858232371957 2312 74-75 -1.5023600373193204 2312 76-77 -2.1508695224836103 2312 78-79 -1.624325717476463 2312 80-81 -2.2122553385953196 2312 82-83 -2.4665756804438708 2312 84-85 -2.395990860652905 2312 86-87 -2.5494229560621733 2312 88-89 -2.947576187141081 2312 90-91 -2.9026750722726256 2312 92-93 -2.993391831464905 2312 94-95 -3.277722301675901 2312 96-97 -2.752303909027429 2312 98-99 -3.11084822661838 2312 100-101 -2.762898756324212 2313 1 -0.23835807792854524 2313 2 -0.05410967541751788 2313 3 -0.026944618638435003 2313 4 -0.07407298385201244 2313 5 -0.028065128165842168 2313 6 0.027356308025510145 2313 7 0.050632865779029146 2313 8 -0.32833912023120604 2313 9 0.4074363817217943 2313 10-11 0.041849153116750415 2313 12-13 0.08043497194092453 2313 14-15 -0.15586345612450003 2313 16-17 -0.4821057776198501 2313 18-19 -0.626876646233633 2313 20-21 0.05624557832047827 2313 22-23 0.34054898426797564 2313 24-25 0.08865176168863087 2313 26-27 -0.053282259356841166 2313 28-29 0.2783889093492533 2313 30-31 0.2869493918894932 2313 32-33 -0.11624825739436062 2313 34-35 0.1880643390100616 2313 36-37 0.20396688723472778 2313 38-39 0.2373028904019847 2313 40-41 -0.7366174937803578 2313 42-43 -0.2228253773030886 2313 44-45 -0.4237801551496432 2313 46-47 -0.22034157946931998 2313 48-49 0.04650355718328569 2313 50-51 -0.2968730741774124 2313 52-53 0.0629758418896813 2313 54-55 -0.3170778252720865 2313 56-57 -0.6549770342024956 2313 58-59 -0.7141542241847887 2313 60-61 -1.4510592294104825 2313 62-63 -0.24587990794923797 2313 64-65 -0.4018399987362429 2313 66-67 -1.2922993257033255 2313 68-69 -1.2612090712595752 2313 70-71 -0.6253155101545147 2313 72-73 -0.6025597386834498 2313 74-75 -0.584565066332857 2313 76-77 -0.7765948609749067 2313 78-79 -1.1538918959788127 2313 80-81 -0.1846233960697603 2313 82-83 -1.5567939727898228 2313 84-85 -0.48289331989579054 2313 86-87 -0.3848747383224662 2313 88-89 -0.6693225259086937 2313 90-91 -1.044288777716119 2313 92-93 -1.2585203199976505 2313 94-95 -0.9074542718334051 2313 96-97 -0.37084494246208166 2313 98-99 -0.4342801138800567 2313 100-101 -1.3037940312620364 2314 1 0.0574572178867534 2314 2 -0.08152670283454455 2314 3 0.026446434752617876 2314 4 -0.11014801992705259 2314 5 0.05274295264224094 2314 6 -0.3954431147739115 2314 7 -0.05614924100307661 2314 8 -0.012321804213890175 2314 9 -0.1784222041367869 2314 10-11 0.05700066826825889 2314 12-13 -0.035005143499198255 2314 14-15 -0.4199327201937635 2314 16-17 -0.03621833173239963 2314 18-19 -0.6146111339681184 2314 20-21 -0.44808342600852313 2314 22-23 0.1421362858552797 2314 24-25 0.6102967833336592 2314 26-27 0.429401723327139 2314 28-29 0.6759358068961419 2314 30-31 0.35693496187506213 2314 32-33 -0.25694089808700227 2314 34-35 0.5459286968744266 2314 36-37 0.14336082662867256 2314 38-39 -0.2388875857884969 2314 40-41 0.46035220318933767 2314 42-43 0.4438412893635828 2314 44-45 -0.2888595202290105 2314 46-47 0.3482009890732485 2314 48-49 -0.23127422059448577 2314 50-51 0.3517560744517354 2314 52-53 0.20511148402533053 2314 54-55 -0.22977623797050484 2314 56-57 -0.36998424920971473 2314 58-59 -0.46884397887453844 2314 60-61 -0.7158499942012497 2314 62-63 -0.42336908543841645 2314 64-65 -0.7257938226900684 2314 66-67 -0.8139443473483468 2314 68-69 -0.6832869933374965 2314 70-71 -0.5048248896638938 2314 72-73 0.24808961196590218 2314 74-75 -0.20577718754498164 2314 76-77 -0.21093829531834274 2314 78-79 -0.6870809291678448 2314 80-81 -0.10092931237567626 2314 82-83 0.1538842378883878 2314 84-85 -0.08318192018439063 2314 86-87 0.14109928765156354 2314 88-89 0.25852740194123314 2314 90-91 0.20102146759412776 2314 92-93 0.5033844419071158 2314 94-95 0.8696020052228732 2314 96-97 0.2979862263690869 2314 98-99 0.24032306072312082 2314 100-101 0.5179952905272813 2315 1 -0.44945399955078 2315 2 -0.8098626943284302 2315 3 -0.2826976375493473 2315 4 0.006811044400436117 2315 5 -0.4299790037639255 2315 6 -0.12165257782547911 2315 7 -0.12924865620775705 2315 8 0.052271497221511254 2315 9 -0.4569974300804347 2315 10-11 -0.16907588675566387 2315 12-13 -0.18027361771503791 2315 14-15 -0.4705706787264674 2315 16-17 -0.3209073269477116 2315 18-19 0.045941763426888826 2315 20-21 -0.40781229363213356 2315 22-23 -0.4326909337087841 2315 24-25 0.24267317886794615 2315 26-27 -0.10130003105881968 2315 28-29 -0.13879040256690445 2315 30-31 0.457945062885166 2315 32-33 -0.35183722982543486 2315 34-35 -0.41526215905327746 2315 36-37 0.09072924768130264 2315 38-39 0.050984822504965166 2315 40-41 -0.1803973980865763 2315 42-43 0.7133787694273792 2315 44-45 0.2316083160283 2315 46-47 0.19416058503284717 2315 48-49 -0.2748680536620043 2315 50-51 0.0796933418626864 2315 52-53 0.2388701230471213 2315 54-55 0.09093083273656788 2315 56-57 0.2949546130975662 2315 58-59 -0.4112948029043153 2315 60-61 0.126919809094872 2315 62-63 0.13285101025536505 2315 64-65 0.5853438700265734 2315 66-67 0.8467414580743053 2315 68-69 1.382901204429647 2315 70-71 1.4483914846051107 2315 72-73 1.280784986766541 2315 74-75 0.76471723031786 2315 76-77 1.0001196525817129 2315 78-79 1.030489512086806 2315 80-81 1.2446315595009878 2315 82-83 1.0351973692015157 2315 84-85 0.760784055360535 2315 86-87 0.60800518876799 2315 88-89 0.9745029468641455 2315 90-91 0.782095364457497 2315 92-93 0.7629538198975467 2315 94-95 0.5015796766742291 2315 96-97 0.1767741051569658 2315 98-99 0.4423432627433215 2315 100-101 -0.089793119892704 2316 1 -1.1155808551513218 2316 2 -1.265598186906029 2316 3 -1.538433130126947 2316 4 -3.5007463105253436 2316 5 -2.674918275018989 2316 6 -1.4018145211453188 2316 7 -0.8963201811740191 2316 8 -1.4726947645868549 2316 9 -1.8827734084879921 2316 10-11 -1.9983606370930431 2316 12-13 -2.562122470616522 2316 14-15 -2.459909410170461 2316 16-17 -1.9472905928046629 2316 18-19 -1.9597937291507108 2316 20-21 -2.2817664096915067 2316 22-23 -1.4970634033444057 2316 24-25 -1.8003092772724045 2316 26-27 -2.357777763852347 2316 28-29 -2.3441885132281755 2316 30-31 -1.7369267319866282 2316 32-33 -1.7909735321196365 2316 34-35 -1.5386089876632596 2316 36-37 -1.4925366092687682 2316 38-39 -0.9499598468607573 2316 40-41 -1.2054986126614793 2316 42-43 -0.9910071954849045 2316 44-45 -0.751252682622173 2316 46-47 -0.5814804406081819 2316 48-49 -0.5779719672922354 2316 50-51 -0.7825873598916999 2316 52-53 -1.0767843978705542 2316 54-55 -0.7577871159813796 2316 56-57 -1.0305514597769232 2316 58-59 -1.1224958825264437 2316 60-61 -0.572337950689203 2316 62-63 -0.17814764021697016 2316 64-65 -0.2931986400948823 2316 66-67 0.3239344405304436 2316 68-69 0.3646151045646029 2316 70-71 0.5088503240113198 2316 72-73 0.12221548609177546 2316 74-75 0.09690346513567505 2316 76-77 -0.14407737845742474 2316 78-79 0.16004416795725263 2316 80-81 -0.2810769425233062 2316 82-83 0.2492999333040835 2316 84-85 0.02070308370061369 2316 86-87 0.15368670023897124 2316 88-89 0.48327487668870717 2316 90-91 0.7889779555506138 2316 92-93 0.09277838130105209 2316 94-95 0.43160666722753405 2316 96-97 0.4165176949005591 2316 98-99 -0.11919519879513985 2316 100-101 -0.8819658594338691 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 400.0 3 139.0 4 98.0 5 84.0 6 115.0 7 120.0 8 144.0 9 192.0 10 246.0 11 332.0 12 512.0 13 690.0 14 804.0 15 1110.0 16 1516.0 17 2222.0 18 1937.0 19 1099.0 20 990.0 21 977.0 22 1002.0 23 1062.0 24 1215.0 25 1309.0 26 1456.0 27 1584.0 28 1727.0 29 1986.0 30 2462.0 31 2989.0 32 3847.0 33 5046.0 34 7199.0 35 10674.0 36 16173.0 37 25384.0 38 25080.0 39 5535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.056678848189385 19.44201929985861 15.68944070585872 20.811861146093285 2 14.582480319522878 21.066415333389987 42.169912625634446 22.181191721452688 3 18.21012214247638 27.616097157734533 31.675152002101374 22.498628697687714 4 11.511625211525534 17.62596876762713 39.712711622120736 31.149694398726595 5 12.091233005881762 37.946252598873116 35.34312854084385 14.619385854401273 6 27.443934405959723 38.017959536946876 19.214540733528075 15.323565323565324 7 24.567949876777835 35.57759906057586 22.53922636568012 17.315224696966187 8 24.672715188797355 33.87223913816288 21.29167375075349 20.16337192228628 9 26.19877437153698 16.862823891254454 22.086041281886818 34.852360455321744 10-11 22.93778588206206 27.36084497465694 28.566571887748793 21.134797255532202 12-13 25.49581747580318 25.623708054015417 28.753356644707612 20.127117825473796 14-15 22.55529888050188 25.89370562375901 29.128583900550865 22.422411595188244 16-17 19.208838754539133 29.621416982152514 29.637641968631694 21.53210229467666 18-19 19.01116008483055 27.411161630013638 34.056221486543386 19.521456798612423 20-21 20.264455020241666 26.436617324391975 33.74215828672085 19.556769368645508 22-23 19.724217844727693 26.317497103128623 33.74391657010429 20.214368482039397 24-25 19.414070798169213 26.42552287518588 34.23495104381916 19.925455282825748 26-27 19.408374981654138 26.427694137821828 34.163853634797654 20.00007724572638 28-29 19.137759890617094 26.249579965006742 34.93030315904939 19.682356985326777 30-31 19.26508415921889 26.697281722888683 34.39288721351491 19.644746904377516 32-33 18.884648956796465 27.00780954293704 33.99700286581645 20.110538634450048 34-35 19.268560216906 26.679515205821236 33.79346037680465 20.25846420046811 36-37 19.565570034837823 26.599952107649642 33.890017534779886 19.944460322732645 38-39 19.402345198368558 26.81837844518601 33.90456989247312 19.874706463972313 40-41 19.22337146697359 27.118270931660703 33.99043697907413 19.66792062229157 42-43 19.03064337965502 27.589083633947954 33.30912967240087 20.071143313996153 44-45 19.230709810979707 27.845925674162075 32.7050680921078 20.218296422750413 46-47 19.145040998327623 27.78539509410497 31.996462132067528 21.07310177549988 48-49 19.543856938704568 27.95759144104129 31.66621605963462 20.83233556061952 50-51 20.142595610897825 28.021273473045106 31.36485473941154 20.471276176645528 52-53 19.771272753957923 28.224370536593657 31.437829987910902 20.566526721537517 54-55 19.444980881387355 28.13371441813758 31.58974160905334 20.831563091421728 56-57 19.586040152328575 28.744293471963662 30.836107742339152 20.83355863336861 58-59 19.602488886142343 27.889506432712395 31.21574595131145 21.292258729833804 60-61 19.59637704949692 27.557598346883992 31.645198045615185 21.2008265580039 62-63 20.025259937892997 27.106926012328703 31.839881347814668 21.027932701963632 64-65 20.040863462734297 26.88298295553298 32.1492713712434 20.92688221048932 66-67 19.24477869064587 27.245443062910645 32.20621806088276 21.30356018556072 68-69 19.39533464407251 27.721747326053453 32.0568433331917 20.826074696682337 70-71 19.352047163178106 27.51211144850606 31.44379283434938 21.69204855396645 72-73 19.12311198671148 27.084250782245917 31.241163518368293 22.551473712674316 74-75 19.40048285852245 27.10883631096089 31.564268469338487 21.926412361178173 76-77 19.62902434407007 26.688456946810852 31.83312663475395 21.849392074365127 78-79 19.040371459143742 26.44367271729935 31.749790436163185 22.766165387393723 80-81 19.058769885891593 27.021627658053472 31.868046941716997 22.051555514337938 82-83 19.06572242119375 27.45961650652356 31.278710819028632 22.195950253254054 84-85 18.94993838392033 26.59226380180869 30.802245220407865 23.655552593863117 86-87 19.19362813262878 26.409876085411454 31.093754828342757 23.302740953617008 88-89 19.621446229913474 25.379712608158222 31.53237021013597 23.46647095179234 90-91 19.085293538181016 25.390656689549136 32.542426571994795 22.981623200275052 92-93 18.519505806541343 25.84432442456132 32.007062114152816 23.62910765474452 94-95 18.12275483796207 26.439800687550697 31.8061725056974 23.631271968789832 96-97 17.958830505135566 26.733132211324893 31.580146862844316 23.72789042069522 98-99 18.65089790745835 27.670926961950975 30.171854232992303 23.506320897598368 100-101 18.827202231692663 27.75860936491807 29.074210735770833 24.339977667618435 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 129.0 1 102.5 2 83.5 3 90.0 4 83.0 5 93.5 6 139.5 7 288.0 8 528.5 9 655.0 10 512.5 11 372.0 12 402.5 13 394.5 14 398.5 15 431.0 16 428.5 17 405.0 18 433.0 19 497.0 20 542.0 21 576.0 22 631.0 23 744.5 24 870.0 25 1036.5 26 1244.5 27 1426.5 28 1621.0 29 1883.5 30 2126.0 31 2334.0 32 2644.0 33 2935.0 34 3302.0 35 3649.0 36 3965.0 37 4277.5 38 4299.5 39 4232.0 40 4141.0 41 4089.0 42 4095.0 43 4156.0 44 4261.5 45 4333.0 46 4471.0 47 4727.0 48 5797.5 49 5524.5 50 4144.5 51 3748.0 52 3405.0 53 3207.0 54 2995.5 55 2667.5 56 2371.5 57 2098.5 58 1832.5 59 1583.5 60 1257.5 61 1002.0 62 795.0 63 565.5 64 396.5 65 277.5 66 210.0 67 159.5 68 116.5 69 84.0 70 64.0 71 46.5 72 21.0 73 12.5 74 16.5 75 11.5 76 10.5 77 6.5 78 1.5 79 1.5 80 2.0 81 2.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020083888858848886 2 0.010814401693226321 3 0.013904230748433843 4 0.030898290552075203 5 0.05716183752133914 6 0.04248514950910341 7 0.012359316220830082 8 0.04557497856431093 9 0.041712692245301525 10-11 0.025491089705462044 12-13 0.03823663455819307 14-15 0.018538974331245125 16-17 0.020856346122650762 18-19 0.017380288435542304 20-21 0.016221602539839484 22-23 0.005407200846613161 24-25 0.0034760576871084607 26-27 0.0 28-29 0.0027036004233065803 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.724572638018801E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.8622863190094006E-4 48-49 0.0015449145276037603 50-51 0.0 52-53 0.0011586858957028203 54-55 0.0015449145276037603 56-57 0.0 58-59 0.0011586858957028203 60-61 0.0034760576871084607 62-63 0.0023173717914056406 64-65 0.0011586858957028203 66-67 0.008883258533721622 68-69 0.010428173061325381 70-71 0.026263546969263928 72-73 0.016994059803641364 74-75 0.015062916644136663 76-77 0.032056976447778024 78-79 0.016607831171740424 80-81 0.04827857898761751 82-83 0.032056976447778024 84-85 0.020470117490749822 86-87 0.010041944429424443 88-89 0.013131773484631964 90-91 0.021242574754551706 92-93 0.026263546969263928 94-95 0.009269487165622562 96-97 0.012745544852731022 98-99 0.03476057687108461 100-101 0.037464177294391186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 129457.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.81894830492175 #Duplication Level Percentage of deduplicated Percentage of total 1 91.771976140953 72.33370643294286 2 5.275998628336182 8.316973262873349 3 1.2750253659814266 3.0148847522626196 4 0.5400001556262485 1.7024897740381988 5 0.3099999240264178 1.2216933993183945 6 0.19799994860383793 0.9363688628029838 7 0.12599996724368445 0.6951829433221258 8 0.09199997608170468 0.5801073087070335 9 0.0549999857010031 0.39015369267649186 >10 0.3219999162858701 4.285385105216301 >50 0.015999995840291683 0.8654318273914839 >100 0.01499999610027345 2.88240819013721 >500 9.999997400182302E-4 0.6250340975605162 >1k 0.0019999994800364604 2.1501803507504262 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1548 1.1957638443653105 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.9114995712862186 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 793 0.612558610194891 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 455 0.35146805502985545 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 400 0.308982905520752 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 359 0.277312157704875 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 354 0.2734498713858656 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 264 0.20392871764369636 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 248 0.1915694014228663 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.19002448689526252 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 239 0.18461728604864935 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 234 0.18075499972963996 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 221 0.17071305530021552 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 179 0.13826985022053656 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.8622863190094006E-4 0.0 22-23 0.0 0.0 0.0 0.0030898290552075205 0.0 24-25 0.0 0.0 0.0 0.006952115374216921 0.0 26-27 0.0 0.0 0.0 0.010041944429424443 0.0 28-29 0.0 0.0 0.0 0.016607831171740424 0.0 30-31 0.0 0.0 0.0 0.027036004233065805 0.0 32-33 0.0 0.0 0.0 0.04016777771769777 0.0 34-35 0.0 0.0 0.0 0.049823493515221265 0.0 36-37 0.0 0.0 0.0 0.059865437944645716 0.0 38-39 0.0 0.0 0.0 0.07376966869307955 0.0 40-41 0.0 0.0 0.0 0.08999127123291904 0.0 42-43 0.0 0.0 0.0 0.14058722201194218 0.0 44-45 0.0 0.0 0.0 0.20122511722038977 0.0 46-47 0.0 0.0 0.0 0.24641386715279978 0.0 48-49 0.0 0.0 0.0 0.31014159141645486 0.0 50-51 0.0 0.0 0.0 0.3792765165267232 0.0 52-53 0.0 0.0 0.0 0.5206361958024672 0.0 54-55 0.0 0.0 0.0 0.6160346678819995 0.0 56-57 0.0 0.0 0.0 0.8477718470225635 0.0 58-59 0.0 0.0 0.0 1.218551333647466 0.0 60-61 0.0 0.0 0.0 1.5777439613153401 0.0 62-63 0.0 0.0 0.0 2.1319820480931893 0.0 64-65 0.0 0.0 0.0 2.4046594622152533 0.0 66-67 0.0 0.0 0.0 2.9419034891894604 0.0 68-69 0.0 0.0 0.0 3.535923125053106 0.0 70-71 0.0 0.0 0.0 3.9997837119661357 0.0 72-73 0.0 0.0 0.0 4.928663571687896 0.0 74-75 0.0 0.0 0.0 5.784546219980379 0.0 76-77 0.0 0.0 0.0 6.264628409433248 0.0 78-79 0.0 0.0 0.0 6.748572885205126 0.0 80-81 0.0 0.0 0.0 7.691743204307222 0.0 82-83 0.0 0.0 0.0 8.202337455680265 0.0 84-85 0.0 0.0 0.0 9.167522806800715 0.0 86-87 0.0 0.0 0.0 11.095962365882107 0.0 88-89 0.0 0.0 0.0 13.347675289864588 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACTAGT 25 0.0049971263 57.0406 2 AGTACAC 50 0.0016048924 38.027065 2 TCTAGGA 110 6.8876034E-8 34.57006 2 GTCCAGG 75 3.0576345E-4 31.676975 1 GTATCAA 1360 0.0 30.39592 1 GGTATCA 950 0.0 29.009441 1 AAGTATC 70 0.008335533 27.162191 7 CCGAAGG 35 0.008374366 27.146446 64-65 TATCAAC 1635 0.0 25.293234 2 CTAGGAC 95 0.0012156179 25.01781 3 ATCAACG 1655 0.0 24.987576 3 TCAACGC 1665 0.0 24.837502 4 GAGTACT 870 0.0 24.576963 12-13 CAACGCA 1695 0.0 24.397898 5 CATGGGA 510 0.0 24.232935 4 AACGCAG 1740 0.0 24.0401 6 TGGGAAC 120 1.6912183E-4 23.766918 6 TACATGG 800 0.0 23.766916 2 ACATGGG 760 0.0 23.141474 3 GTACATG 815 0.0 22.737461 1 >>END_MODULE