FastQCFastQC Report
Thu 26 May 2016
SRR936925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129457
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14321.1061588017642925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.9787033532369822No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.560803973520165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.42639640961863784No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4950.38236634558193067No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4550.35146805502985545No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG3510.27113249959445995No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3210.2479587816804035No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG3200.24718632441660163No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.23096472187676217No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG2650.20470117490749823No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.2016113458522907No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.1792100852020362No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA1850.14290459380334783No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1520.11741350409788578No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1310.10119190155804629No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1300.10041944429424442No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG405.3672417E-447.5056041
GCGAATG205.387524E-447.48725516-17
TATCCTC500.001612648137.9898035
GTATAGG600.003935846531.6704031
GTGCTGA650.00582182129.2342171
CTAGCAT650.005832829529.2229254
ATCTAAT700.00835974927.1460591
TACGTTA350.00839075427.13557238-39
GAGTACT11100.024.1714412-13
GTACTTT11450.023.0178414-15
AGTACTT11050.022.77669112-13
GTATCAA23300.022.0197641
ACTTTTT13350.020.09760316-17
TACTTTT12750.019.55357614-15
TATCAAC26500.019.1740992
TCAACGC27050.018.7842374
ATCAACG27100.018.749583
CAACGCA27450.018.5105155
AACGCAG27750.018.3104026
GGTATCA18000.018.210481
Produced by FastQC (version 0.11.5)