##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936925_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 129457 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.394841530392334 33.0 31.0 34.0 31.0 34.0 2 32.5085935870598 34.0 31.0 34.0 31.0 34.0 3 32.58284217925643 34.0 31.0 34.0 31.0 34.0 4 36.19124496937207 37.0 35.0 37.0 35.0 37.0 5 36.13243779787883 37.0 35.0 37.0 35.0 37.0 6 36.19847516936125 37.0 36.0 37.0 35.0 37.0 7 36.16641046834084 37.0 36.0 37.0 35.0 37.0 8 36.14956317541732 37.0 36.0 37.0 35.0 37.0 9 37.85847038012622 39.0 38.0 39.0 35.0 39.0 10-11 37.78835829657724 39.0 37.5 39.0 35.0 39.0 12-13 37.702403114547685 39.0 37.0 39.0 35.0 39.0 14-15 38.90233050356489 40.0 38.0 41.0 35.5 41.0 16-17 38.825219957205874 40.0 38.0 41.0 35.5 41.0 18-19 39.006226005546246 40.0 38.5 41.0 36.0 41.0 20-21 39.058733788053175 40.0 38.5 41.0 35.0 41.0 22-23 38.99645828344546 40.0 38.0 41.0 35.0 41.0 24-25 38.86583962242289 40.0 38.0 41.0 35.0 41.0 26-27 38.673088361386405 40.0 38.0 41.0 35.0 41.0 28-29 38.55365488154368 40.0 38.0 41.0 34.5 41.0 30-31 38.4633662142642 40.0 38.0 41.0 35.0 41.0 32-33 38.209417026503004 40.0 37.0 41.0 34.0 41.0 34-35 38.01276871857065 40.0 37.0 41.0 34.0 41.0 36-37 37.82375228840465 40.0 37.0 41.0 33.5 41.0 38-39 37.61054635902269 40.0 36.5 41.0 33.0 41.0 40-41 37.37007655051484 40.0 36.0 41.0 33.0 41.0 42-43 37.04408413604517 40.0 36.0 41.0 32.5 41.0 44-45 36.61871895687371 40.0 35.5 41.0 31.0 41.0 46-47 36.01456081942267 39.5 35.0 41.0 29.5 41.0 48-49 35.453895888209985 39.0 35.0 41.0 27.0 41.0 50-51 35.06180816796311 39.0 35.0 40.5 25.0 41.0 52-53 34.74964273851549 39.0 35.0 40.0 23.5 41.0 54-55 34.38379539151997 38.0 35.0 40.0 22.0 41.0 56-57 34.10469499524939 38.0 34.0 40.0 20.5 41.0 58-59 33.76002456414099 38.0 34.0 40.0 18.5 41.0 60-61 33.42434939786956 38.0 33.5 40.0 15.0 41.0 62-63 33.038514719173165 37.0 33.0 40.0 12.0 41.0 64-65 32.60867315015797 36.5 33.0 39.0 7.5 41.0 66-67 32.20466255204431 36.0 32.0 39.0 4.5 40.0 68-69 31.895359076759078 35.5 32.0 39.0 2.0 40.0 70-71 31.4041612272801 35.0 31.0 38.0 2.0 40.0 72-73 30.871486285021284 35.0 31.0 37.5 2.0 40.0 74-75 30.370227179681287 35.0 30.5 37.0 2.0 39.0 76-77 28.963057231358675 33.5 28.5 35.5 2.0 38.0 78-79 29.579597858748464 34.0 30.0 36.0 2.0 38.5 80-81 29.22398557049831 34.0 29.0 36.0 2.0 37.5 82-83 28.933186308967457 34.0 29.0 35.0 2.0 37.0 84-85 28.659520149547728 34.0 29.0 35.0 2.0 36.5 86-87 28.376233034907344 34.0 29.0 35.0 2.0 36.0 88-89 27.97725885815367 34.0 28.5 35.0 2.0 36.0 90-91 27.54652123871247 34.0 27.0 35.0 2.0 35.0 92-93 27.143808368802 33.5 26.5 35.0 2.0 35.0 94-95 26.637142062615386 33.0 25.5 35.0 2.0 35.0 96-97 26.04095182184046 33.0 24.5 35.0 2.0 35.0 98-99 25.32977359277598 32.5 21.0 34.0 2.0 35.0 100-101 22.249700672810278 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.316442916218179 1101 2 0.14032204765995715 1101 3 0.09998455391917105 1101 4 0.24613827272759892 1101 5 0.23628125008089995 1101 6 0.18500662494483322 1101 7 0.2108603308079111 1101 8 0.2384029465596953 1101 9 0.26035534656691794 1101 10-11 0.268734847079827 1101 12-13 0.1414442778506242 1101 14-15 0.2689611184353211 1101 16-17 0.21492992595216975 1101 18-19 0.08875346973343312 1101 20-21 0.20515955055500257 1101 22-23 0.23652537930337303 1101 24-25 0.12693831184945736 1101 26-27 0.06825943025947367 1101 28-29 0.2762170622419404 1101 30-31 0.41294103887577194 1101 32-33 0.29665150196995427 1101 34-35 0.029555073683731337 1101 36-37 0.06433039100360105 1101 38-39 0.13404755535850654 1101 40-41 -0.19006144699475414 1101 42-43 0.19330155295338614 1101 44-45 0.466109804954435 1101 46-47 0.31574367520036617 1101 48-49 0.0327900066787592 1101 50-51 -0.0619887519932405 1101 52-53 0.35922036777859034 1101 54-55 0.20722742580685605 1101 56-57 0.2974763182472344 1101 58-59 0.5202729687470082 1101 60-61 0.7115518133561949 1101 62-63 0.7411179125480842 1101 64-65 0.5848630550853535 1101 66-67 0.6591657367750088 1101 68-69 0.6796299549066802 1101 70-71 0.5697830811084188 1101 72-73 0.6664137813277335 1101 74-75 0.5882547100363276 1101 76-77 0.32000820946643316 1101 78-79 0.43134618028290106 1101 80-81 0.5851843534576773 1101 82-83 0.8851550570967568 1101 84-85 0.49417058832705507 1101 86-87 -0.16705727223350664 1101 88-89 0.21424482824140867 1101 90-91 0.4331387236238413 1101 92-93 0.4814697571461011 1101 94-95 -0.03830773887908734 1101 96-97 -0.11544874639390557 1101 98-99 0.035953857959849955 1101 100-101 0.9148928957863696 1102 1 0.18847139061734453 1102 2 0.2990537613485671 1102 3 0.1908120095095569 1102 4 -0.04467938662454429 1102 5 0.1050932929231152 1102 6 -0.10248031340417896 1102 7 0.08705220646099576 1102 8 0.10205563725980227 1102 9 0.2811396987090333 1102 10-11 0.10272126296280248 1102 12-13 0.22551232905230023 1102 14-15 0.1958485270037542 1102 16-17 0.3131453778852915 1102 18-19 0.2623183208306159 1102 20-21 0.1301856738569782 1102 22-23 -0.05343510920236838 1102 24-25 -0.11227766254970106 1102 26-27 -0.5056776778910006 1102 28-29 -0.21913788812378954 1102 30-31 -0.05548241984941171 1102 32-33 -0.16478397347414386 1102 34-35 -0.3991119848324658 1102 36-37 -0.12689217952931386 1102 38-39 -0.13271217557148418 1102 40-41 -0.026766318990581794 1102 42-43 0.10056383090532961 1102 44-45 0.10623682951033686 1102 46-47 0.6204801563915865 1102 48-49 0.758021850983873 1102 50-51 0.6700922955511714 1102 52-53 0.4010503050826628 1102 54-55 0.5812559655142735 1102 56-57 0.8209872900340685 1102 58-59 0.43521713018901664 1102 60-61 0.45735575797479555 1102 62-63 0.6104687484937514 1102 64-65 0.37069279646466313 1102 66-67 0.5826244619578631 1102 68-69 -0.14793992492612773 1102 70-71 -0.2464966341475865 1102 72-73 0.5499528356642038 1102 74-75 0.6836292528785393 1102 76-77 0.46472313893351824 1102 78-79 0.5612442994051605 1102 80-81 0.9341508503228795 1102 82-83 1.1770258120601866 1102 84-85 1.2986866541577733 1102 86-87 0.8720052277664934 1102 88-89 1.3174726887429777 1102 90-91 1.6203366729446387 1102 92-93 1.6010246813885303 1102 94-95 0.9420916211000154 1102 96-97 0.8399538789979424 1102 98-99 1.1741297983987202 1102 100-101 1.5352641732158396 1103 1 0.1336706659796576 1103 2 0.04834206362600213 1103 3 -0.048480174755454186 1103 4 0.16171295291997012 1103 5 0.14640411072850412 1103 6 0.14777848576766672 1103 7 -0.018505505754326634 1103 8 -0.2395269196760168 1103 9 0.10182422251855172 1103 10-11 0.1930421739358792 1103 12-13 0.14674033112269314 1103 14-15 0.2435322744157702 1103 16-17 0.15526170811303786 1103 18-19 -0.1003078437656626 1103 20-21 0.03442998027519906 1103 22-23 0.06614628313717219 1103 24-25 -0.22134533853314053 1103 26-27 0.287266809686642 1103 28-29 -0.06241881462482013 1103 30-31 0.4791319693845466 1103 32-33 0.11970101617388451 1103 34-35 -0.19876907592976778 1103 36-37 -0.6144698192808633 1103 38-39 -0.29014294679301145 1103 40-41 0.012619679974221754 1103 42-43 -0.3223140158855671 1103 44-45 -0.14562328953728354 1103 46-47 0.2134052340313346 1103 48-49 0.18876072261949162 1103 50-51 -0.25361047359997 1103 52-53 0.05535931129384153 1103 54-55 -0.40281171046915887 1103 56-57 -0.3436089832578517 1103 58-59 0.07259096559273814 1103 60-61 -0.012138289644248346 1103 62-63 0.3981887277898082 1103 64-65 0.5690138368373319 1103 66-67 0.49307994850030923 1103 68-69 0.3738315554051326 1103 70-71 -0.14490178425110756 1103 72-73 0.42789667624391114 1103 74-75 0.6319179433547326 1103 76-77 0.6008353283745116 1103 78-79 0.6571293925728625 1103 80-81 0.9998049652297141 1103 82-83 0.812782152639894 1103 84-85 0.6132246976360314 1103 86-87 0.3515762681391621 1103 88-89 0.11051422601005711 1103 90-91 -0.18111583740733295 1103 92-93 0.1903912704154429 1103 94-95 -0.495812752605989 1103 96-97 -0.6536893663436736 1103 98-99 -0.5489874376261241 1103 100-101 -0.33337907212577633 1104 1 0.2679937483409205 1104 2 0.08081189142986744 1104 3 0.2785914810542707 1104 4 0.217007605245378 1104 5 0.004102439264578095 1104 6 -0.014065679257832642 1104 7 0.023154848737412692 1104 8 0.0381582795362192 1104 9 0.049559414156185255 1104 10-11 0.07934727922295792 1104 12-13 0.3968791989709928 1104 14-15 0.08837901480863763 1104 16-17 -0.12035309772446112 1104 18-19 -0.23634783770597068 1104 20-21 0.04278729987324681 1104 22-23 0.04673003307404855 1104 24-25 0.3054687805397265 1104 26-27 0.33927913105209484 1104 28-29 0.4414947338274331 1104 30-31 0.21255619803677206 1104 32-33 0.35274651433072535 1104 34-35 -0.11138332629587211 1104 36-37 -0.14721738278134922 1104 38-39 -0.12337528532758313 1104 40-41 -0.06976682712065951 1104 42-43 -0.1900865755987411 1104 44-45 -0.4249496542295006 1104 46-47 -0.14012705905556544 1104 48-49 -0.20602794576408456 1104 50-51 -0.3854844320911113 1104 52-53 -0.5966631095514856 1104 54-55 -0.042664258062963256 1104 56-57 0.1339954201153688 1104 58-59 0.13167292287194243 1104 60-61 0.25092791244633617 1104 62-63 0.31956427694903056 1104 64-65 0.48127460540775147 1104 66-67 0.2757138928521776 1104 68-69 0.5117399531226496 1104 70-71 0.33994899593371386 1104 72-73 0.33793555924143703 1104 74-75 0.1853441919029315 1104 76-77 0.25480189909611894 1104 78-79 -0.2126378144159773 1104 80-81 0.09186172430661799 1104 82-83 0.21469095433660712 1104 84-85 0.15475880862931746 1104 86-87 0.15814597979901635 1104 88-89 -0.09023818524076077 1104 90-91 -0.32611911973829066 1104 92-93 -0.35038537958708105 1104 94-95 -0.05441498459104466 1104 96-97 0.243472679810953 1104 98-99 0.7563198390491266 1104 100-101 -0.037843042231312296 1105 1 0.2731454830265818 1105 2 -0.022625281555718857 1105 3 -0.006846818873285088 1105 4 0.15808463977809595 1105 5 0.2099303391277374 1105 6 0.15288272289945581 1105 7 0.25232654639498975 1105 8 0.08911215541161255 1105 9 0.5219090881479715 1105 10-11 0.3407575665931546 1105 12-13 0.2775440947288672 1105 14-15 0.344879055056559 1105 16-17 -0.10530037293978722 1105 18-19 0.43148601885042126 1105 20-21 0.5220998652761253 1105 22-23 0.35345948650719805 1105 24-25 0.5563743589024384 1105 26-27 0.7648204656733526 1105 28-29 0.69002568448348 1105 30-31 0.6940019035829366 1105 32-33 0.6884544753707402 1105 34-35 1.0013108699530164 1105 36-37 1.0760781175003942 1105 38-39 0.7676333277288521 1105 40-41 0.3333564120325292 1105 42-43 0.4710258771099447 1105 44-45 0.6054375308304643 1105 46-47 0.7769622108470386 1105 48-49 0.3611055968584651 1105 50-51 0.15279134670628025 1105 52-53 0.11113693874602859 1105 54-55 0.29570006617434075 1105 56-57 0.14771996330139814 1105 58-59 0.015275917317730148 1105 60-61 0.30610782068106346 1105 62-63 0.37727980459936106 1105 64-65 0.4860238608210956 1105 66-67 0.35572677218889126 1105 68-69 0.3539680783742014 1105 70-71 0.09954812657848677 1105 72-73 0.4847197498556213 1105 74-75 1.101507759479201 1105 76-77 0.9060133617057957 1105 78-79 0.9393383588110993 1105 80-81 1.0613940433921876 1105 82-83 1.1567339391228906 1105 84-85 1.2709948224746022 1105 86-87 1.4765070842021366 1105 88-89 1.3115166243865417 1105 90-91 0.669815839611303 1105 92-93 1.0575273629066793 1105 94-95 1.4238108867765824 1105 96-97 1.4846785077108144 1105 98-99 2.0591854914680248 1105 100-101 1.978256128661382 1106 1 -0.18718789208308095 1106 2 -0.5001287281029079 1106 3 -0.1727823997731477 1106 4 -0.3007447874684246 1106 5 0.08735858828176646 1106 6 -0.24541280285565392 1106 7 0.1281255186972814 1106 8 0.3583188229138088 1106 9 -0.31228065994076104 1106 10-11 -0.22059097332412136 1106 12-13 0.1993388058455423 1106 14-15 0.21892342151853228 1106 16-17 0.1776298188135641 1106 18-19 -0.03505114119469965 1106 20-21 0.19875107470086562 1106 22-23 0.027228129194256212 1106 24-25 -0.35677265200118313 1106 26-27 -0.10427340573910726 1106 28-29 -0.31542612652041413 1106 30-31 0.1208097742434191 1106 32-33 0.08455674382543776 1106 34-35 -0.2662665206974424 1106 36-37 -0.2661326858584303 1106 38-39 -0.8387709835883612 1106 40-41 -0.6845656333365682 1106 42-43 -1.037621823103116 1106 44-45 -0.796617864582494 1106 46-47 -0.6306076600641077 1106 48-49 -1.939433317792492 1106 50-51 -1.8888805420015728 1106 52-53 -1.5210224797274634 1106 54-55 -2.1964668720384637 1106 56-57 -2.210658279586184 1106 58-59 -1.6343042305704785 1106 60-61 -2.2725361196623375 1106 62-63 -2.0834922219703884 1106 64-65 -2.1268348934087555 1106 66-67 -1.5260253270716682 1106 68-69 -0.822056486107563 1106 70-71 -0.9772785434302911 1106 72-73 -0.7867428394412848 1106 74-75 -1.2568704834083775 1106 76-77 -0.8965308167626844 1106 78-79 -0.6974582322404075 1106 80-81 -0.43541006739863874 1106 82-83 -0.7119839453237802 1106 84-85 -0.3454984724245058 1106 86-87 -0.7303484114740115 1106 88-89 -0.7148651277127165 1106 90-91 -0.2036018819076837 1106 92-93 -0.40863381017265255 1106 94-95 -0.4835083261573736 1106 96-97 0.14503694861819483 1106 98-99 -0.17812494843672155 1106 100-101 -1.0536400989360608 1107 1 0.06730910991559114 1107 2 0.20640025257664263 1107 3 0.3007352551235911 1107 4 0.11431184144563389 1107 5 0.22543320520382082 1107 6 -0.17046276954452821 1107 7 0.3203307152329202 1107 8 0.45375519866330905 1107 9 0.56047083905991 1107 10-11 0.05447564892770629 1107 12-13 0.4028697851926495 1107 14-15 0.4036336147230557 1107 16-17 0.47501489542915465 1107 18-19 0.29397950504114334 1107 20-21 0.3626418142078549 1107 22-23 0.2605725662362204 1107 24-25 0.36291422341520274 1107 26-27 0.4544374536879445 1107 28-29 0.8130715417007721 1107 30-31 -0.11195171809502114 1107 32-33 0.023264272139890352 1107 34-35 0.16969283972894544 1107 36-37 0.3467920309970012 1107 38-39 -0.23125932469428534 1107 40-41 0.8056075845181994 1107 42-43 0.4536340063439184 1107 44-45 0.4229857330191109 1107 46-47 0.48931239323369624 1107 48-49 0.47320825449265413 1107 50-51 0.2127183920423903 1107 52-53 0.6000634629774027 1107 54-55 0.6156583777949791 1107 56-57 0.11046097424459589 1107 58-59 0.2826677880837565 1107 60-61 0.3008316351677749 1107 62-63 0.6592626081428676 1107 64-65 0.8859066122541428 1107 66-67 1.6637648128350548 1107 68-69 1.0261280575300162 1107 70-71 1.1870285851506566 1107 72-73 1.034876081278238 1107 74-75 0.8208277177908201 1107 76-77 1.273522481038782 1107 78-79 1.7224285099314756 1107 80-81 1.5834107187439344 1107 82-83 1.742129978726851 1107 84-85 1.3669432331051432 1107 86-87 1.0763637803980721 1107 88-89 1.8326865045324503 1107 90-91 1.340868471190248 1107 92-93 1.6710630585815096 1107 94-95 1.4504523667140496 1107 96-97 1.6791808526821548 1107 98-99 1.4890969036618777 1107 100-101 1.723175357426367 1108 1 -0.02333416493821261 1108 2 0.07335931690412423 1108 3 0.0814370095095569 1108 4 -0.21829049773565146 1108 5 0.14502384847867233 1108 6 0.18918635326249245 1108 7 0.20337165090543863 1108 8 0.051708415037580835 1108 9 0.17523692093125476 1108 10-11 0.567999040740574 1108 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1526.0 17 2370.0 18 2237.0 19 1185.0 20 970.0 21 895.0 22 878.0 23 970.0 24 1137.0 25 1155.0 26 1344.0 27 1576.0 28 1745.0 29 2177.0 30 2584.0 31 3150.0 32 4108.0 33 5481.0 34 7957.0 35 11682.0 36 18549.0 37 28935.0 38 21863.0 39 1665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.42160574916931 20.313731550884782 15.658758983077043 19.605903716868866 2 15.31223928560393 22.803819176219758 40.11680004943917 21.76714148873714 3 19.495427017673958 27.925318254851067 31.368186874304783 21.21106785317019 4 12.530222857363563 18.90927349271948 38.449654320033986 30.11084932988297 5 12.550885622252949 39.05698417235067 33.75638242814216 14.635747777254224 6 28.535343782105254 38.53171323296539 17.931050464633046 15.001892520296314 7 25.270939385278506 36.761241184331475 21.44882084398681 16.518998586403207 8 26.058073337092623 34.79302007616429 19.28053330449493 19.868373282248157 9 27.398286689788886 17.04581444031609 21.101215075275963 34.454683794619065 10-11 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33.017140826683764 19.562480205782613 36-37 20.72710979265165 27.499536479095205 32.634961836778835 19.138391891474306 38-39 20.514415054693778 27.450404795748103 32.92480378221371 19.11037636734442 40-41 19.968483013271126 28.112302439476572 32.87963291980163 19.039581627450676 42-43 20.192419104413048 28.743521014699862 32.05195547556332 19.012104405323775 44-45 20.328371582842177 28.591733162362793 31.50042098920877 19.579474265586256 46-47 20.32729140676598 28.99236423045579 30.442272114571306 20.238072248206926 48-49 20.724510053841936 29.034475832927782 30.136033927371326 20.104980185858963 50-51 21.34293306964959 29.265033063469502 29.742444842716765 19.649589024164143 52-53 20.541569787651497 29.560394571170352 29.91302131209591 19.985014329082244 54-55 20.598607189620743 29.310817648444772 29.9442644099482 20.14631075198628 56-57 20.727067666268834 29.911202264940922 29.363120550939925 19.998609517850316 58-59 20.469810322386284 29.16462286559578 29.71577325201902 20.64979355999892 60-61 20.18184205850772 29.372822568808754 29.814295535831537 20.631039836851986 62-63 20.927546755865244 29.063955689113087 29.93727259384005 20.071224961181624 64-65 20.847543383298635 28.99236423045579 30.276579391533065 19.88351299471251 66-67 20.222327798313618 29.262216248160467 29.961722229303543 20.553733724222372 68-69 20.363983268511042 29.420693437563973 29.998879932641465 20.21644336128352 70-71 20.055231547642038 29.67285929473562 29.53999459271562 20.731914564906724 72-73 20.343434499486314 28.86209320469345 29.310890874962343 21.48358142085789 74-75 20.231120105981546 28.919752967212926 29.305210630597923 21.54391629620761 76-77 20.161134585229075 28.716871239098694 29.761619688390738 21.36037448728149 78-79 20.10938628577167 28.202503679040863 29.749438970409308 21.938671064778156 80-81 20.16809319284368 29.130874764391436 29.15018694187807 21.550845100886814 82-83 19.823922986587554 29.64838679770072 28.825174609061126 21.702515606650596 84-85 19.53305469069895 29.1373409457883 28.474083917272498 22.855520446240256 86-87 19.770091855102244 29.007362313914232 28.097309240360623 23.1252365906229 88-89 20.054690396737115 27.96818996415771 29.00985663082437 22.967263008280806 90-91 19.36696149241049 27.886524274844543 29.83546406087057 22.911050171874397 92-93 19.354452478062043 28.31927450253368 28.856522679520452 23.46975033988382 94-95 18.938334736630697 29.44143615254486 28.52143955135682 23.098789559467622 96-97 18.95203185773713 29.914909560024878 28.181814670472498 22.951243911765502 98-99 19.156669318892863 30.605016569976286 26.756842357339846 23.48147175379101 100-101 19.343949511998236 31.121650902040454 25.796309957398815 23.738089628562488 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 272.0 1 183.0 2 95.0 3 108.5 4 108.5 5 109.5 6 176.5 7 276.0 8 381.0 9 468.5 10 431.0 11 382.5 12 429.5 13 469.5 14 472.0 15 452.5 16 479.5 17 484.5 18 482.5 19 521.5 20 531.0 21 588.0 22 668.0 23 739.0 24 832.0 25 970.5 26 1159.0 27 1369.0 28 1556.0 29 1747.0 30 2048.5 31 2365.0 32 2599.0 33 2843.0 34 3110.5 35 3430.0 36 3855.5 37 4057.0 38 4127.0 39 4115.5 40 4028.5 41 4094.0 42 4047.5 43 4095.5 44 4175.0 45 4210.0 46 4352.5 47 4567.0 48 5830.5 49 5679.5 50 4320.0 51 3900.5 52 3477.5 53 3352.0 54 3173.5 55 2840.5 56 2512.5 57 2227.0 58 1952.5 59 1704.0 60 1402.5 61 1094.0 62 846.0 63 623.5 64 452.0 65 301.0 66 208.0 67 171.0 68 137.5 69 94.5 70 71.5 71 50.0 72 32.0 73 25.0 74 16.5 75 10.0 76 8.5 77 5.0 78 1.5 79 1.0 80 1.0 81 1.5 82 2.0 83 1.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03630549139868836 2 0.0038622863190094004 3 7.724572638018801E-4 4 0.0015449145276037603 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.013904230748433843 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 7.724572638018801E-4 22-23 0.0011586858957028203 24-25 0.011200630325127263 26-27 0.0027036004233065803 28-29 3.8622863190094006E-4 30-31 0.0019311431595047002 32-33 0.0011586858957028203 34-35 0.0 36-37 0.010041944429424443 38-39 0.006952115374216921 40-41 0.0023173717914056406 42-43 0.0 44-45 0.0 46-47 3.8622863190094006E-4 48-49 0.0030898290552075205 50-51 0.006952115374216921 52-53 0.0 54-55 0.005020972214712221 56-57 0.004248514950910341 58-59 3.8622863190094006E-4 60-61 0.0030898290552075205 62-63 0.006179658110415041 64-65 3.8622863190094006E-4 66-67 0.006565886742315982 68-69 3.8622863190094006E-4 70-71 0.0015449145276037603 72-73 0.0 74-75 3.8622863190094006E-4 76-77 0.0 78-79 0.005793429478514101 80-81 0.0038622863190094004 82-83 0.019311431595047 84-85 0.015449145276037602 86-87 0.010814401693226321 88-89 7.724572638018801E-4 90-91 0.0015449145276037603 92-93 7.724572638018801E-4 94-95 0.0 96-97 0.005020972214712221 98-99 0.004634743582811281 100-101 3.8622863190094006E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 129457.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.19794217384923 #Duplication Level Percentage of deduplicated Percentage of total 1 90.15911822412146 67.79780158662722 2 6.236324975089626 9.379176097082428 3 1.5285210942074392 3.448249225611593 4 0.6605101233705534 1.9867600824984357 5 0.36980349053405787 1.3904230748433843 6 0.2198276304841344 0.991835126721614 7 0.16127541114957525 0.8489305329182663 8 0.11094104716021737 0.6674030759248244 9 0.08526024920646334 0.5770255760600045 >10 0.4232195502778663 5.508392748171207 >50 0.024653566035603862 1.2930934596043473 >100 0.01643571069040257 3.0388468757965965 >500 0.0020544638363003214 0.9872003831388029 >1k 0.0020544638363003214 2.0848621550012747 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1432 1.1061588017642925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1267 0.9787033532369822 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.560803973520165 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 552 0.42639640961863784 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 495 0.38236634558193067 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 455 0.35146805502985545 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 351 0.27113249959445995 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 321 0.2479587816804035 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 320 0.24718632441660163 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.23096472187676217 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 265 0.20470117490749823 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 261 0.2016113458522907 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.1792100852020362 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 185 0.14290459380334783 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 152 0.11741350409788578 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 131 0.10119190155804629 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 130 0.10041944429424442 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.8622863190094006E-4 0.0 18-19 0.0 0.0 0.0 7.724572638018801E-4 0.0 20-21 0.0 0.0 0.0 0.00115868589570282 0.0 22-23 0.0 0.0 0.0 0.003862286319009401 0.0 24-25 0.0 0.0 0.0 0.007724572638018802 0.0 26-27 0.0 0.0 0.0 0.010814401693226321 0.0 28-29 0.0 0.0 0.0 0.017380288435542304 0.0 30-31 0.0 0.0 0.0 0.027808461496867685 0.0 32-33 0.0 0.0 0.0 0.041712692245301525 0.0 34-35 0.0 0.0 0.0 0.05214086530662691 0.0 36-37 0.0 0.0 0.0 0.064500181527457 0.0 38-39 0.0 0.0 0.0 0.07840441227589084 0.0 40-41 0.0 0.0 0.0 0.09462601481573032 0.0 42-43 0.0 0.0 0.0 0.14522196559475348 0.0 44-45 0.0 0.0 0.0 0.2081772325946067 0.0 46-47 0.0 0.0 0.0 0.25491089705462044 0.0 48-49 0.0 0.0 0.0 0.31593502089496894 0.0 50-51 0.0 0.0 0.0 0.38700108916474196 0.0 52-53 0.0 0.0 0.0 0.5310643688637926 0.0 54-55 0.0 0.0 0.0 0.6272352982071268 0.0 56-57 0.0 0.0 0.0 0.8647659068262048 0.0 58-59 0.0 0.0 0.0 1.238248993874414 0.0 60-61 0.0 0.0 0.0 1.5986003074379909 0.0 62-63 0.0 0.0 0.0 2.153610851479642 0.0 64-65 0.0 0.0 0.0 2.425515808337904 0.0 66-67 0.0 0.0 0.0 2.9619873780483097 0.0 68-69 0.0 0.0 0.0 3.555234556648154 0.0 70-71 0.0 0.0 0.0 4.016005314505975 0.0 72-73 0.0 0.0 0.0 4.941022887908726 0.0 74-75 0.0 0.0 0.0 5.7926570212502995 0.0 76-77 0.0 0.0 0.0 6.270421838911762 0.0 78-79 0.0 0.0 0.0 6.751662714260333 0.0 80-81 0.0 0.0 0.0 7.691356975675321 0.0 82-83 0.0 0.0 0.0 8.200406312520759 0.0 84-85 0.0 0.0 0.0 9.160184462794597 0.0 86-87 0.0 0.0 0.0 11.08437550692508 0.0 88-89 0.0 0.0 0.0 13.345357918073184 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 40 5.3672417E-4 47.505604 1 GCGAATG 20 5.387524E-4 47.487255 16-17 TATCCTC 50 0.0016126481 37.989803 5 GTATAGG 60 0.0039358465 31.670403 1 GTGCTGA 65 0.005821821 29.234217 1 CTAGCAT 65 0.0058328295 29.222925 4 ATCTAAT 70 0.008359749 27.146059 1 TACGTTA 35 0.008390754 27.135572 38-39 GAGTACT 1110 0.0 24.17144 12-13 GTACTTT 1145 0.0 23.01784 14-15 AGTACTT 1105 0.0 22.776691 12-13 GTATCAA 2330 0.0 22.019764 1 ACTTTTT 1335 0.0 20.097603 16-17 TACTTTT 1275 0.0 19.553576 14-15 TATCAAC 2650 0.0 19.174099 2 TCAACGC 2705 0.0 18.784237 4 ATCAACG 2710 0.0 18.74958 3 CAACGCA 2745 0.0 18.510515 5 AACGCAG 2775 0.0 18.310402 6 GGTATCA 1800 0.0 18.21048 1 >>END_MODULE