FastQCFastQC Report
Thu 26 May 2016
SRR936924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140574
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16811.1958114587334783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12210.8685816722864826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.6003955212201403No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA4940.35141633588003474No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT4630.32936389374991104No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA4380.31157966622561784No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3750.2667634128643988No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.21412209939249077No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2800.19918334827208445No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT2640.18780144265653678No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG2580.18353322805070638No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.18282185894973466No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC2560.18211048984876294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.15436709491086545No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG1410.10030304323701394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGC250.004986363557.0742237
TACAGTA459.534891E-442.2772037
TTTAGAC600.00390739231.719212
ATGGGAG1950.031.719215
GTCCAGG902.3709728E-531.719211
TGCACTG753.0351224E-431.719215
TCGCCCT300.003958640631.64585132-33
GTATCAA17000.030.2265421
TATACAA856.317401E-427.9875375
GTACATG8900.027.2642651
CATGGGG2800.027.1878954
TTAGGGT700.00829951127.1878954
TAGAGCC700.00829951127.1878955
TATACAG700.00829951127.1878955
TATGTAG700.00829951127.1878955
TACATGG8850.026.8806862
GGTATCA11600.026.2503811
TATCAAC19550.026.0405792
GAGTACT10350.025.50418312-13
ATCAACG20250.025.3753683
Produced by FastQC (version 0.11.5)