##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936924_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 140574 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90944982713731 33.0 31.0 34.0 30.0 34.0 2 31.99898274218561 33.0 31.0 34.0 30.0 34.0 3 32.002418654943305 34.0 31.0 34.0 30.0 34.0 4 35.59960590151806 37.0 35.0 37.0 33.0 37.0 5 35.59074224251995 37.0 35.0 37.0 33.0 37.0 6 35.66863004538535 37.0 35.0 37.0 35.0 37.0 7 35.635956862577714 37.0 35.0 37.0 33.0 37.0 8 35.608049852746596 37.0 35.0 37.0 33.0 37.0 9 37.246510734559735 39.0 37.0 39.0 34.0 39.0 10-11 37.13817633417275 39.0 37.0 39.0 34.0 39.0 12-13 37.064720360806405 39.0 37.0 39.0 33.5 39.0 14-15 38.18897164482763 40.0 38.0 41.0 33.5 41.0 16-17 38.158692219044774 40.0 38.0 41.0 33.0 41.0 18-19 38.18002262153741 40.0 38.0 41.0 33.5 41.0 20-21 38.14820308165095 40.0 38.0 41.0 34.0 41.0 22-23 38.061700598972784 40.0 38.0 41.0 33.5 41.0 24-25 38.01386102693243 40.0 38.0 41.0 33.0 41.0 26-27 37.99980081665173 40.0 38.0 41.0 33.0 41.0 28-29 37.88164952267134 40.0 38.0 41.0 33.0 41.0 30-31 37.650226926743215 40.0 37.0 41.0 33.0 41.0 32-33 37.35546046921906 40.0 36.0 41.0 33.0 41.0 34-35 37.142750437492 40.0 36.0 41.0 32.0 41.0 36-37 36.92646933287806 39.0 35.0 41.0 31.5 41.0 38-39 36.70422339835247 39.0 35.0 41.0 31.0 41.0 40-41 36.44115199112211 39.0 35.0 41.0 30.5 41.0 42-43 36.16685873632393 39.0 35.0 41.0 30.0 41.0 44-45 35.77106008223427 39.0 35.0 41.0 29.0 41.0 46-47 35.21420746368461 39.0 35.0 40.0 26.0 41.0 48-49 34.7634662170814 38.5 35.0 40.0 23.5 41.0 50-51 33.991872608021396 38.0 34.0 40.0 21.5 40.5 52-53 34.129863986227896 38.5 34.5 40.0 22.0 40.5 54-55 34.45284334229658 39.0 35.0 40.5 20.5 41.0 56-57 34.30908987437222 39.0 35.0 41.0 18.5 41.0 58-59 34.0925348926544 38.5 34.5 41.0 15.5 41.0 60-61 33.7654402663366 38.0 34.0 40.0 11.0 41.0 62-63 33.41270078392875 38.0 34.0 40.0 7.5 41.0 64-65 33.02924794058645 37.0 33.5 40.0 4.0 41.0 66-67 32.63787044545933 37.0 33.0 40.0 2.0 41.0 68-69 32.241449343406316 36.0 33.0 39.0 2.0 41.0 70-71 31.77802438573278 36.0 32.0 39.0 2.0 41.0 72-73 31.346877801015836 35.0 31.5 39.0 2.0 40.0 74-75 30.90773187075846 35.0 31.0 37.5 2.0 40.0 76-77 30.43794371647673 35.0 31.0 37.0 2.0 39.0 78-79 29.961973764707558 35.0 30.5 36.5 2.0 39.0 80-81 29.536404313742228 35.0 30.0 36.0 2.0 39.0 82-83 29.131468123550583 34.5 29.5 36.0 2.0 37.0 84-85 28.74395336264174 34.0 29.0 35.0 2.0 37.0 86-87 28.345006900280282 34.0 29.0 35.0 2.0 36.5 88-89 27.9590073555565 34.0 28.5 35.0 2.0 36.0 90-91 27.550304465975216 34.0 27.0 35.0 2.0 36.0 92-93 27.186965583962895 34.0 26.0 35.0 2.0 35.5 94-95 26.83723874969767 34.0 25.5 35.0 2.0 35.0 96-97 26.350648057250986 34.0 25.0 35.0 2.0 35.0 98-99 25.81769388364847 34.0 22.0 35.0 2.0 35.0 100-101 23.852735925562335 31.0 9.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38286149981238893 1101 2 0.3368132085239175 1101 3 0.531845408242031 1101 4 0.3521154740969976 1101 5 0.4056904172508311 1101 6 0.46533203934990297 1101 7 0.34921183563161406 1101 8 0.5796790990362979 1101 9 0.387048512545185 1101 10-11 0.36201693930605217 1101 12-13 0.5650289032584439 1101 14-15 0.6028161198950954 1101 16-17 0.6322104496585297 1101 18-19 0.6202715713198685 1101 20-21 0.5848244841403698 1101 22-23 0.5888234195122095 1101 24-25 0.6701710524185103 1101 26-27 0.4395709155805392 1101 28-29 0.5476211000850171 1101 30-31 0.46914692994617013 1101 32-33 0.7280195453119447 1101 34-35 0.9519341333484803 1101 36-37 1.029065896253087 1101 38-39 0.673180419553141 1101 40-41 0.8342816822731223 1101 42-43 1.00733807599088 1101 44-45 0.9989492375247622 1101 46-47 1.2822980638442232 1101 48-49 1.3535928969870241 1101 50-51 1.4023586462398043 1101 52-53 1.5072152594779098 1101 54-55 1.3277624881015058 1101 56-57 1.5671334909931574 1101 58-59 1.5225865665170843 1101 60-61 1.7378585190573688 1101 62-63 1.651020744846683 1101 64-65 1.3359133360687068 1101 66-67 1.6620646761384599 1101 68-69 1.4984016259418205 1101 70-71 1.4800676479944848 1101 72-73 1.1233387693342998 1101 74-75 1.4395931711355097 1101 76-77 1.620517389503842 1101 78-79 1.4201060414082427 1101 80-81 1.5691270936132682 1101 82-83 1.5623871239591303 1101 84-85 1.3289878396464552 1101 86-87 1.6730548520766249 1101 88-89 1.8007376210662471 1101 90-91 1.6927029191024836 1101 92-93 1.677207891032051 1101 94-95 1.3841436979948867 1101 96-97 0.8395655710672578 1101 98-99 0.9292225268991068 1101 100-101 0.8838479469193992 1102 1 -0.18842936503531504 1102 2 -0.05104577321988302 1102 3 -0.1228855685174608 1102 4 -0.15262784587431355 1102 5 -0.26458125096363005 1102 6 -0.4161025945269259 1102 7 -0.682927536863005 1102 8 -0.5014804575485172 1102 9 -0.3241673525964117 1102 10-11 -0.22613122625755722 1102 12-13 -0.17301287557471312 1102 14-15 -0.3181461804093715 1102 16-17 0.07808252696230511 1102 18-19 -0.6201212954792439 1102 20-21 -0.6498258503018945 1102 22-23 -0.40966600646966356 1102 24-25 -0.4307905425595635 1102 26-27 -0.25455841776846455 1102 28-29 -0.8313566468673912 1102 30-31 -0.40046173146189545 1102 32-33 -0.3405381797753364 1102 34-35 -0.5154797874433612 1102 36-37 -0.724757560551545 1102 38-39 -0.49257128204677514 1102 40-41 -0.5987240661369526 1102 42-43 -0.05142736771819756 1102 44-45 0.0847992146016594 1102 46-47 -0.20396570471467612 1102 48-49 0.6358116187004939 1102 50-51 0.5598462736393799 1102 52-53 1.1657432433024226 1102 54-55 0.36265263351629784 1102 56-57 0.6936514121611879 1102 58-59 0.656345838620247 1102 60-61 0.3242316132066847 1102 62-63 -0.07128050005439945 1102 64-65 0.6449403383139938 1102 66-67 0.4806077069259551 1102 68-69 0.715201325002564 1102 70-71 0.7917161133218151 1102 72-73 1.131955506729529 1102 74-75 1.4008002196972313 1102 76-77 1.3950539312885546 1102 78-79 1.1741058298103226 1102 80-81 1.3495295998730406 1102 82-83 1.2799861929282983 1102 84-85 0.3804766661008969 1102 86-87 0.7095496150281804 1102 88-89 0.3683725441268919 1102 90-91 0.3405628412814714 1102 92-93 0.33292632473724737 1102 94-95 0.08767150548780123 1102 96-97 8.610194291946982E-5 1102 98-99 0.25175065178889966 1102 100-101 0.1499088165868372 1103 1 0.1206929868885922 1103 2 0.28293606854405695 1103 3 0.301749891137451 1103 4 0.24266752023817162 1103 5 0.0306866026699808 1103 6 -0.11529294157719505 1103 7 0.15097569781843845 1103 8 0.07584925985902657 1103 9 -0.18318555013600957 1103 10-11 -0.09181137403887618 1103 12-13 -6.96324853485919E-4 1103 14-15 -0.17775393106771276 1103 16-17 0.3268837689542039 1103 18-19 0.24487104033021012 1103 20-21 -0.44074083674217945 1103 22-23 -0.2960296428333038 1103 24-25 -0.14449372221605472 1103 26-27 -0.2874653076792484 1103 28-29 0.22811943692776993 1103 30-31 0.004698823504106997 1103 32-33 0.1493018548117817 1103 34-35 -0.39854192634207664 1103 36-37 -0.3439848529305962 1103 38-39 -0.48594863534631116 1103 40-41 -0.046558441014134644 1103 42-43 0.18290034520873633 1103 44-45 0.058220194831974936 1103 46-47 0.6183626370689126 1103 48-49 0.34369787425211484 1103 50-51 0.7467542640493221 1103 52-53 0.667954067497682 1103 54-55 0.7510207504836401 1103 56-57 0.8538526462923812 1103 58-59 0.7962369678109908 1103 60-61 0.5312531908508262 1103 62-63 0.4429277301071437 1103 64-65 0.4567058533869002 1103 66-67 0.2360217697330178 1103 68-69 0.6781675632606259 1103 70-71 0.39813045125366386 1103 72-73 0.34020532003770754 1103 74-75 0.22262744132990164 1103 76-77 0.6626913954240727 1103 78-79 0.8048294080047214 1103 80-81 0.4541556266780553 1103 82-83 0.5349679167816142 1103 84-85 0.3358769726685189 1103 86-87 0.8085945389758287 1103 88-89 -0.04021724481308908 1103 90-91 0.15229887869844205 1103 92-93 0.04590238888062714 1103 94-95 -0.33232613629972363 1103 96-97 0.2205887942354927 1103 98-99 0.0725363295784689 1103 100-101 -0.1552438776709124 1104 1 -0.20240642831608824 1104 2 0.07679571766686522 1104 3 0.08398008784133282 1104 4 0.1936244580372133 1104 5 -0.33208585878669084 1104 6 -0.10824881611201675 1104 7 -0.13856444040590787 1104 8 -0.3779652005343834 1104 9 -0.12211431143318663 1104 10-11 -0.3702536919548791 1104 12-13 -0.08665459173281675 1104 14-15 -0.8211284658896076 1104 16-17 -0.26239719861624167 1104 18-19 -0.09294793893614184 1104 20-21 -0.21546172988411882 1104 22-23 -0.8340081117328211 1104 24-25 -0.547470862035297 1104 26-27 -0.26237891746127673 1104 28-29 -0.6078388299515538 1104 30-31 0.06048696810804444 1104 32-33 -0.0396601334923119 1104 34-35 -0.015966168766318845 1104 36-37 0.236690319849842 1104 38-39 0.18028608023050907 1104 40-41 0.22643464776843558 1104 42-43 0.5937190586590333 1104 44-45 0.5930420980749318 1104 46-47 0.8097169698706139 1104 48-49 1.6277223293292025 1104 50-51 1.4140854708542108 1104 52-53 1.3910401918995987 1104 54-55 1.6229306388408986 1104 56-57 1.8982771545326784 1104 58-59 1.7453664202299208 1104 60-61 2.154458932477624 1104 62-63 1.9697419246844916 1104 64-65 1.4897334982566477 1104 66-67 1.9908729127420521 1104 68-69 1.4958177493318665 1104 70-71 1.7134215198342098 1104 72-73 1.449920896858547 1104 74-75 2.2992489192671677 1104 76-77 2.0276568393368812 1104 78-79 1.492096818956341 1104 80-81 1.692825217321328 1104 82-83 1.965988650433932 1104 84-85 1.9042581528918028 1104 86-87 1.7015371227078866 1104 88-89 1.500053887563304 1104 90-91 1.0052029191024836 1104 92-93 1.2317078115281532 1104 94-95 1.5165574362680587 1104 96-97 1.5843580658995045 1104 98-99 1.095097467271188 1104 100-101 1.8992816406703952 1105 1 -0.10152923533362923 1105 2 0.1718249574329498 1105 3 0.27818086756842675 1105 4 0.18290321047386016 1105 5 0.24954182152519877 1105 6 0.03453870825445193 1105 7 0.5171373139800579 1105 8 0.5472297312394971 1105 9 0.49021512326466876 1105 10-11 0.5096196023920996 1105 12-13 0.24509492093775265 1105 14-15 0.4779699746561974 1105 16-17 0.769644711715145 1105 18-19 0.9654097608689298 1105 20-21 0.9426766716753363 1105 22-23 0.6551488083451531 1105 24-25 0.014176311453979906 1105 26-27 -0.04125318646713083 1105 28-29 0.20076253457086324 1105 30-31 0.13054057434585786 1105 32-33 0.5289314844414079 1105 34-35 0.4979227201225669 1105 36-37 -0.007705391651128934 1105 38-39 0.18576853637085833 1105 40-41 -0.0671813366370273 1105 42-43 -0.5763589140505232 1105 44-45 0.1571259187376981 1105 46-47 0.047655566361839874 1105 48-49 0.33654299209723604 1105 50-51 0.4740269913220416 1105 52-53 0.5467419462855645 1105 54-55 -0.03727891281602069 1105 56-57 0.1998122422519799 1105 58-59 0.2873985839726103 1105 60-61 0.014839049436687901 1105 62-63 0.23838227556169045 1105 64-65 0.38641965810069934 1105 66-67 0.27937193808318384 1105 68-69 0.30990156999463636 1105 70-71 0.4823055354287469 1105 72-73 0.524969629802019 1105 74-75 0.8577284514309085 1105 76-77 0.33735469508737026 1105 78-79 0.6188024719777871 1105 80-81 0.4911926637150934 1105 82-83 1.0690588258725242 1105 84-85 1.337981349772896 1105 86-87 1.3014396568209463 1105 88-89 1.1862137316178902 1105 90-91 0.5491844005839646 1105 92-93 1.1330236010018382 1105 94-95 1.8053001263265394 1105 96-97 1.7824574811041813 1105 98-99 2.0615935686357076 1105 100-101 2.0897056172785824 1106 1 0.8015715398601664 1106 2 0.7558042855983196 1106 3 0.5370094636063278 1106 4 0.5280797823946202 1106 5 0.664701166917105 1106 6 0.7072346600201769 1106 7 0.8640365387862587 1106 8 0.6613554849001346 1106 9 0.7929713678813783 1106 10-11 0.9586936764661758 1106 12-13 0.9830363507396527 1106 14-15 0.745196503510634 1106 16-17 0.6497050045661368 1106 18-19 1.0198886583710802 1106 20-21 1.0330082823557802 1106 22-23 0.7182409702745218 1106 24-25 0.5134226508165938 1106 26-27 0.03788118045793709 1106 28-29 0.21185211252090852 1106 30-31 0.5877972496257868 1106 32-33 0.6680357049409835 1106 34-35 0.888103598675535 1106 36-37 0.6396890958596302 1106 38-39 0.2516600092390817 1106 40-41 -0.31212104378603556 1106 42-43 0.4012465984387106 1106 44-45 -0.015893030444040335 1106 46-47 0.5980431632610674 1106 48-49 0.9949193917527026 1106 50-51 1.5386264745261755 1106 52-53 2.1436411710917653 1106 54-55 1.675791715952279 1106 56-57 1.0389595290736793 1106 58-59 1.7605897984428864 1106 60-61 1.1152912458191153 1106 62-63 1.4496225856392044 1106 64-65 0.042212078428008226 1106 66-67 0.6244408441985989 1106 68-69 1.479905876626848 1106 70-71 1.129807688744858 1106 72-73 0.722105719379968 1106 74-75 1.1099894333430527 1106 76-77 1.0325528001691957 1106 78-79 1.2714725839502208 1106 80-81 1.4728894768072536 1106 82-83 1.2444464227717482 1106 84-85 1.020690221435256 1106 86-87 1.8112372451069092 1106 88-89 1.6181689426428676 1106 90-91 1.902005244683881 1106 92-93 1.746395694025093 1106 94-95 2.284305294285197 1106 96-97 2.0127546387269213 1106 98-99 2.0116366349578456 1106 100-101 1.5864756431183764 1107 1 0.3889469551425613 1107 2 0.5305551161631037 1107 3 0.43003271942027865 1107 4 0.25565453322518294 1107 5 -0.009717437734060752 1107 6 0.46205193576768266 1107 7 0.46237540921815423 1107 8 0.3101926942024562 1107 9 0.21190824495779026 1107 10-11 0.6801222478947508 1107 12-13 0.3305446563874881 1107 14-15 0.6385519852382089 1107 16-17 0.585253177323608 1107 18-19 0.5810182264773971 1107 20-21 0.9713803753790415 1107 22-23 0.26176256495891437 1107 24-25 0.34049906277672903 1107 26-27 0.02342935321541262 1107 28-29 0.6807889895973176 1107 30-31 0.5132125320178176 1107 32-33 0.321524077034006 1107 34-35 -0.2623947401948925 1107 36-37 -0.10466306360880395 1107 38-39 0.37624472684705523 1107 40-41 0.05199855754286631 1107 42-43 0.2955987579071504 1107 44-45 -0.1866571500453702 1107 46-47 -0.6142491955429108 1107 48-49 -0.4643829338286878 1107 50-51 -0.8362904689954149 1107 52-53 -1.2151628156192018 1107 54-55 -0.8617498122869307 1107 56-57 -0.4597115672718317 1107 58-59 -0.391569669995647 1107 60-61 0.017616827214460784 1107 62-63 0.04314418032359413 1107 64-65 -0.5946649979839513 1107 66-67 -0.3747285910173481 1107 68-69 -0.012452927359863253 1107 70-71 -0.20208599896278656 1107 72-73 0.13356751339990325 1107 74-75 0.4930459117483714 1107 76-77 1.3626192453519224 1107 78-79 1.3090141121894234 1107 80-81 1.390663563185992 1107 82-83 1.0892969211106198 1107 84-85 0.7341453709369183 1107 86-87 0.9306724610537529 1107 88-89 1.0515576469618004 1107 90-91 1.1498457762453391 1107 92-93 1.4151664581446965 1107 94-95 0.8477604437868571 1107 96-97 0.17571144935815042 1107 98-99 0.7809057379478759 1107 100-101 1.2127214902944559 1108 1 0.24385229077078563 1108 2 0.3692814500701651 1108 3 0.4553339242395573 1108 4 0.08439807883620176 1108 5 0.1408849719044909 1108 6 0.4049090786248257 1108 7 -0.011979151883394934 1108 8 0.4379035375759557 1108 9 0.3434056632021907 1108 10-11 0.16351295357460316 1108 12-13 -0.16532453466242458 1108 14-15 0.45800121071152944 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-3.1210417973950975 2316 98-99 -2.9671462101040724 2316 100-101 -3.7320837045107425 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 426.0 3 140.0 4 107.0 5 118.0 6 127.0 7 125.0 8 176.0 9 151.0 10 255.0 11 375.0 12 597.0 13 744.0 14 906.0 15 1181.0 16 1659.0 17 2338.0 18 2210.0 19 1200.0 20 1055.0 21 1094.0 22 1079.0 23 1261.0 24 1332.0 25 1461.0 26 1568.0 27 1681.0 28 1956.0 29 2166.0 30 2645.0 31 3239.0 32 4184.0 33 5532.0 34 7806.0 35 11794.0 36 17526.0 37 27046.0 38 27401.0 39 5910.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0263613006996 19.55533097524002 15.702197012291027 20.716110711769353 2 14.512513609484298 20.902033061262248 42.18904552150121 22.396407807752247 3 18.27794776915961 27.484522877677364 31.62598733366541 22.611542019497616 4 11.775425508438984 17.70229002199617 39.549683582599535 30.972600886965314 5 12.041593960329049 37.965884405826 35.18321997079876 14.809301663046186 6 27.70678405561729 38.10510869874918 18.9011881357381 15.286919109895432 7 25.02634051883703 35.31409288948373 22.37093145769855 17.288635133980694 8 24.77408512486737 34.01362966338862 21.144492946613592 20.06779226513042 9 26.297952325349588 16.811437359382563 21.810298180161194 35.08031213510666 10-11 23.03151778816543 27.312924322419175 28.359601123575505 21.29595676583989 12-13 25.492353041695146 25.612326270221008 28.776130667578038 20.11919002050581 14-15 22.563684517791796 26.02877728573666 28.9449515157556 22.462586680715944 16-17 19.30712450262302 29.764675735121397 29.35502929096642 21.573170471289156 18-19 19.21210848369861 27.602188468890493 33.630088955493775 19.555614091917118 20-21 20.483377233573005 26.480031323414256 33.55769915284402 19.47889229016872 22-23 19.858950956635603 26.317886968619792 33.40343086497508 20.41973120976953 24-25 19.50825620193368 26.633276655686856 33.88244249827477 19.976024644104694 26-27 19.689408517434313 26.371820545562546 33.815772157031525 20.122998779971617 28-29 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31.210315695864825 20.739172965762563 54-55 19.463337934174767 28.048650340794286 31.41710659248403 21.070905132546923 56-57 19.706245397856424 28.7141744243541 30.537957235192216 21.041622942597265 58-59 19.636117879310653 28.015721984100168 31.143045156241662 21.20511498034752 60-61 19.547981603406146 27.529087035238796 31.590067546658414 21.332863814696644 62-63 20.196001707455892 27.274473534433696 31.55129482071713 20.978229937393284 64-65 20.04090488724479 27.143060396955253 31.90616774560717 20.909866970192788 66-67 19.288733296332662 27.491141186013746 31.82520030169776 21.394925215955826 68-69 19.48238191147898 27.884334811293225 31.72216726330698 20.911116013920818 70-71 19.292189095982263 27.709188231148946 31.22190626345741 21.776716409411378 72-73 19.32096679531186 27.288313443719208 30.95345652444555 22.43726323652338 74-75 19.573700629986803 27.037141119178138 31.28377152573484 22.105386725100225 76-77 19.75273503513297 26.77470426042871 31.416170061371517 22.0563906430668 78-79 19.10257869869642 26.58429156941994 31.549211589912908 22.76391814197074 80-81 19.136666061492786 27.213957445596648 31.509980029261936 22.139396463648627 82-83 19.24564418400276 27.60326050216857 30.932075243452488 22.21902007037618 84-85 19.069979225362133 26.92378838327784 30.380773499530438 23.62545889182959 86-87 19.451827656266126 26.682037979953243 30.711314638685185 23.15481972509545 88-89 19.695395345527007 25.536616610917374 31.378905415984626 23.389082627570993 90-91 19.365903996014662 25.532505426466923 32.319681172828524 22.78190940468989 92-93 18.741125855387473 25.790977641603767 31.722731688569567 23.74516481443919 94-95 18.267517101785415 26.312737673018844 31.76254219863328 23.657203026562463 96-97 18.097860502180765 26.676841199029504 31.805730467388134 23.419567831401594 98-99 18.582505684709467 27.705441307821232 30.21062782678628 23.501425180683018 100-101 18.688560777877072 28.007206257766132 29.23198524568568 24.072247718671115 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 128.0 1 99.0 2 80.5 3 93.0 4 90.0 5 90.5 6 149.5 7 330.5 8 552.5 9 649.5 10 557.5 11 419.0 12 371.0 13 406.0 14 428.0 15 438.0 16 456.5 17 469.5 18 507.5 19 528.0 20 582.5 21 638.0 22 689.0 23 826.0 24 964.0 25 1100.5 26 1314.0 27 1564.0 28 1769.0 29 1956.5 30 2218.0 31 2535.0 32 2910.5 33 3208.5 34 3569.5 35 3858.5 36 4173.0 37 4606.5 38 4632.0 39 4535.5 40 4518.5 41 4470.5 42 4385.5 43 4449.5 44 4615.0 45 4671.5 46 4739.0 47 4997.0 48 6336.0 49 6210.0 50 4625.0 51 4102.0 52 3740.5 53 3501.5 54 3329.5 55 2973.0 56 2620.5 57 2349.0 58 2049.0 59 1728.5 60 1387.0 61 1129.0 62 905.5 63 683.0 64 467.5 65 317.5 66 250.5 67 191.5 68 127.0 69 80.5 70 56.5 71 43.5 72 29.5 73 18.5 74 13.0 75 7.5 76 4.5 77 4.5 78 4.5 79 4.0 80 2.0 81 1.5 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04623899156316246 2 0.03343434774567132 3 0.03130024044275612 4 0.06758006459231437 5 0.1202213780642224 6 0.1323146527807418 7 0.0754051247030034 8 0.10314851964090088 9 0.08678703031855109 10-11 0.09212229857583906 12-13 0.089632506722438 14-15 0.0825188157127207 16-17 0.06188911178454053 18-19 0.07860628565737618 20-21 0.07398238650105994 22-23 0.03948098510393103 24-25 0.009247798312632493 26-27 0.0024897918534010554 28-29 0.009603482863118358 30-31 0.006758006459231436 32-33 0.009959167413604222 34-35 0.008180744661174898 36-37 0.009603482863118358 38-39 0.007113691009717302 40-41 0.008892113762146627 42-43 0.004623899156316246 44-45 0.0024897918534010554 46-47 0.003912530055344515 48-49 7.113691009717302E-4 50-51 0.003912530055344515 52-53 0.008180744661174898 54-55 0.014227382019434603 56-57 0.010314851964090087 58-59 0.004623899156316246 60-61 0.003201160954372786 62-63 0.009959167413604222 64-65 0.002845476403886921 66-67 0.025609287634982287 68-69 0.04623899156316246 70-71 0.0601106890321112 72-73 0.004623899156316246 74-75 0.011026221065061818 76-77 0.025964972185468154 78-79 0.027032025836925745 80-81 0.08429723846515003 82-83 0.030944555892270267 84-85 0.012804643817491143 86-87 0.03734687780101584 88-89 0.045527622462190734 90-91 0.04197077695733208 92-93 0.04801741431559179 94-95 0.013160328367977009 96-97 0.019206965726236716 98-99 0.04659467611364833 100-101 0.09994735868652808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 140574.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.74517887063989 #Duplication Level Percentage of deduplicated Percentage of total 1 90.64528456927144 69.56588578048792 2 5.919207079986239 9.08541212251804 3 1.5186344168425683 3.4964360987907845 4 0.6576580919535714 2.0188835161080236 5 0.32180210495880435 1.2348380053005936 6 0.22485955318942383 1.03541319781767 7 0.15690184610347072 0.8429022171041131 8 0.10393497056830117 0.6381206325743173 9 0.06895685478115018 0.47628955390824995 >10 0.34578364822886865 4.533570933979093 >50 0.016989369999312193 0.8919883261968584 >100 0.016989369999312193 3.307182856888094 >500 9.993747058418938E-4 0.647324288314831 >1k 0.0019987494116837875 2.2257524700114213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1681 1.1958114587334783 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1221 0.8685816722864826 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 844 0.6003955212201403 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 494 0.35141633588003474 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 463 0.32936389374991104 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 438 0.31157966622561784 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 375 0.2667634128643988 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.21412209939249077 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 280 0.19918334827208445 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 264 0.18780144265653678 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 258 0.18353322805070638 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.18282185894973466 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 256 0.18211048984876294 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.15436709491086545 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 141 0.10030304323701394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0014227382019434604 0.0 18-19 0.0 0.0 0.0 0.0014227382019434604 0.0 20-21 0.0 0.0 0.0 0.0014227382019434604 0.0 22-23 0.0 0.0 0.0 0.004623899156316246 0.0 24-25 0.0 0.0 0.0 0.009247798312632493 0.0 26-27 0.0 0.0 0.0 0.011737590166033549 0.0 28-29 0.0 0.0 0.0 0.021341073029151905 0.0 30-31 0.0 0.0 0.0 0.03379003229615719 0.0 32-33 0.0 0.0 0.0 0.04659467611364833 0.0 34-35 0.0 0.0 0.0 0.06331184998648398 0.0 36-37 0.0 0.0 0.0 0.07825060110689033 0.0 38-39 0.0 0.0 0.0 0.09496777497972597 0.0 40-41 0.0 0.0 0.0 0.10457125784284435 0.0 42-43 0.0 0.0 0.0 0.15187730305746439 0.0 44-45 0.0 0.0 0.0 0.20345156287791483 0.0 46-47 0.0 0.0 0.0 0.25253603084496423 0.0 48-49 0.0 0.0 0.0 0.31229103532658953 0.0 50-51 0.0 0.0 0.0 0.39125300553445164 0.0 52-53 0.0 0.0 0.0 0.5399291476375432 0.0 54-55 0.0 0.0 0.0 0.6558823110959353 0.0 56-57 0.0 0.0 0.0 0.9027273891331256 0.0 58-59 0.0 0.0 0.0 1.2217764309189465 0.0 60-61 0.0 0.0 0.0 1.531933358942621 0.0 62-63 0.0 0.0 0.0 2.0476759571471255 0.0 64-65 0.0 0.0 0.0 2.3162177927639536 0.0 66-67 0.0 0.0 0.0 2.8188000626004808 0.0 68-69 0.0 0.0 0.0 3.3526825728797642 0.0 70-71 0.0 0.0 0.0 3.7770142416094012 0.0 72-73 0.0 0.0 0.0 4.631368531876449 0.0 74-75 0.0 0.0 0.0 5.365857128629761 0.0 76-77 0.0 0.0 0.0 5.795879750167172 0.0 78-79 0.0 0.0 0.0 6.240129753724017 0.0 80-81 0.0 0.0 0.0 7.109067110560986 0.0 82-83 0.0 0.0 0.0 7.597777682928564 0.0 84-85 0.0 0.0 0.0 8.419408994550913 0.0 86-87 0.0 0.0 0.0 10.201388592485095 0.0 88-89 0.0 0.0 0.0 12.470656024584915 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGGTGC 25 0.0049863635 57.074223 7 TACAGTA 45 9.534891E-4 42.277203 7 TTTAGAC 60 0.003907392 31.71921 2 ATGGGAG 195 0.0 31.71921 5 GTCCAGG 90 2.3709728E-5 31.71921 1 TGCACTG 75 3.0351224E-4 31.71921 5 TCGCCCT 30 0.0039586406 31.645851 32-33 GTATCAA 1700 0.0 30.226542 1 TATACAA 85 6.317401E-4 27.987537 5 GTACATG 890 0.0 27.264265 1 CATGGGG 280 0.0 27.187895 4 TTAGGGT 70 0.008299511 27.187895 4 TAGAGCC 70 0.008299511 27.187895 5 TATACAG 70 0.008299511 27.187895 5 TATGTAG 70 0.008299511 27.187895 5 TACATGG 885 0.0 26.880686 2 GGTATCA 1160 0.0 26.250381 1 TATCAAC 1955 0.0 26.040579 2 GAGTACT 1035 0.0 25.504183 12-13 ATCAACG 2025 0.0 25.375368 3 >>END_MODULE