FastQCFastQC Report
Thu 26 May 2016
SRR936924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140574
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15471.1004879992032666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.9247798312632493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.5939931993113947No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5630.4005008038470841No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5600.3983666965441689No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT5210.37062330160627144No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG4300.30588871341784396No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA3290.23404043421969925No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3200.22763811231095366No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG3040.21625620669540596No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG2970.21127662298860383No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770.1970492409691693No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.1650376314254414No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA1880.13373739098268528No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1630.11595316345839202No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC1590.1131076870545051No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA1520.10812810334770298No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTTA250.00500975457.0068329
ATCCATA459.573753E-442.2423172
GCTTATA500.001607228838.018081
CCCCGTA250.001612788938.00455568-69
AATCCTT801.0726322E-535.6165925
AGGTAAG550.002565960434.5618972
GTAATCC550.002574938334.5373043
ACGCATT300.003936735431.68173634-35
TCTACGC300.003936735431.68173632-33
CGTATTA300.00394360331.67046470-71
ACCGTTA300.00394360331.67046478-79
ACCCCGT300.00394360331.67046466-67
GGTAAGG600.00394381831.6591973
CGAAATC300.003950480431.65919756-57
TATTCCG300.003950480431.65919752-53
ACGAAAT300.003950480431.65919754-55
TAATCCT753.0690635E-431.6591954
CTGAACT856.374542E-427.9445259
CGCATTC350.00836154227.15577536-37
TACGCAT350.00836154227.15577534-35
Produced by FastQC (version 0.11.5)