##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936924_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 140574 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.30709804088949 33.0 31.0 34.0 31.0 34.0 2 32.427860059470454 34.0 31.0 34.0 31.0 34.0 3 32.52381663750053 34.0 31.0 34.0 31.0 34.0 4 36.16165151450482 37.0 35.0 37.0 35.0 37.0 5 36.036941397413464 37.0 35.0 37.0 35.0 37.0 6 36.15730504929788 37.0 35.0 37.0 35.0 37.0 7 36.11371946448134 37.0 35.0 37.0 35.0 37.0 8 36.081800332920736 37.0 35.0 37.0 35.0 37.0 9 37.79272127135886 39.0 38.0 39.0 35.0 39.0 10-11 37.72924224963364 39.0 37.0 39.0 35.0 39.0 12-13 37.62798597180133 39.0 37.0 39.0 35.0 39.0 14-15 38.79192453796577 40.0 38.0 41.0 35.0 41.0 16-17 38.7933366056312 40.0 38.0 41.0 35.5 41.0 18-19 38.96213382275528 40.0 38.0 41.0 35.0 41.0 20-21 38.9903396076088 40.0 38.0 41.0 35.0 41.0 22-23 38.892117318992135 40.0 38.0 41.0 35.0 41.0 24-25 38.79394838305804 40.0 38.0 41.0 35.0 41.0 26-27 38.58039537894632 40.0 38.0 41.0 34.5 41.0 28-29 38.396300169305846 40.0 38.0 41.0 34.0 41.0 30-31 38.299582426337736 40.0 37.5 41.0 34.0 41.0 32-33 38.07884103746069 40.0 37.0 41.0 34.0 41.0 34-35 37.91532929275684 40.0 37.0 41.0 34.0 41.0 36-37 37.70390328225703 40.0 36.5 41.0 33.0 41.0 38-39 37.48571570845249 40.0 36.0 41.0 33.0 41.0 40-41 37.00384139314525 40.0 35.5 41.0 32.0 41.0 42-43 36.76555764223825 39.0 35.0 41.0 31.0 41.0 44-45 36.42288403260916 39.0 35.0 41.0 31.0 41.0 46-47 35.85188583948668 39.0 35.0 41.0 29.0 41.0 48-49 35.28346280250971 39.0 35.0 40.0 26.5 41.0 50-51 34.916759144649795 39.0 35.0 40.0 24.0 41.0 52-53 34.60458192837936 38.5 35.0 40.0 23.5 41.0 54-55 34.20352981347902 38.0 34.0 40.0 22.0 41.0 56-57 33.949514134904035 38.0 34.0 40.0 20.5 41.0 58-59 33.60240869577589 38.0 34.0 40.0 18.5 41.0 60-61 33.203440892341405 37.0 33.0 40.0 14.5 41.0 62-63 32.791472818586655 37.0 33.0 39.5 10.0 41.0 64-65 32.43527252550258 36.0 32.0 39.0 7.5 40.0 66-67 32.06486974831761 36.0 32.0 39.0 4.0 40.0 68-69 31.608921279895288 35.0 31.0 38.5 2.0 40.0 70-71 31.148370253389672 35.0 31.0 38.0 2.0 40.0 72-73 30.72392832244939 35.0 31.0 37.5 2.0 39.5 74-75 30.239646022735357 35.0 30.0 37.0 2.0 39.0 76-77 28.83369613157483 33.5 28.5 35.5 2.0 38.0 78-79 29.392935393458252 34.0 29.0 36.0 2.0 38.0 80-81 29.258628907194787 34.0 29.0 35.5 2.0 37.0 82-83 28.899593808243345 34.0 29.0 35.0 2.0 37.0 84-85 28.490264913853203 34.0 29.0 35.0 2.0 36.5 86-87 28.123116650305178 34.0 29.0 35.0 2.0 36.0 88-89 27.71395492765376 34.0 27.5 35.0 2.0 35.5 90-91 27.287382446256064 33.0 26.5 35.0 2.0 35.0 92-93 26.820948397285417 33.0 25.5 35.0 2.0 35.0 94-95 26.354244028056396 33.0 25.0 34.5 2.0 35.0 96-97 25.683611478651812 32.0 23.5 34.0 2.0 35.0 98-99 24.90340318977905 32.0 18.5 34.0 2.0 35.0 100-101 22.060327656607907 28.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0562987662584149 1101 2 0.1319466040732422 1101 3 0.17176221156675808 1101 4 0.1176957491819337 1101 5 -0.043471658484442344 1101 6 0.07772023555883578 1101 7 0.3147459818155838 1101 8 0.12567166720755552 1101 9 -0.01489388525404678 1101 10-11 0.04454907672575814 1101 12-13 0.03942194533637178 1101 14-15 -0.002630040805932765 1101 16-17 0.2417777465380908 1101 18-19 0.09340860889579261 1101 20-21 0.047571557570584844 1101 22-23 0.16325539512957477 1101 24-25 0.10254634454633305 1101 26-27 0.07011449185295504 1101 28-29 0.23791350930567035 1101 30-31 0.08310165617515963 1101 32-33 0.2783409810278741 1101 34-35 0.020013480965083374 1101 36-37 0.1909520443394399 1101 38-39 -0.11782537641122559 1101 40-41 0.4731339074005305 1101 42-43 0.23475652253365098 1101 44-45 0.2530462593403797 1101 46-47 -0.09930643103020032 1101 48-49 -0.4066631600589261 1101 50-51 -0.4167834627212912 1101 52-53 0.16110922172899222 1101 54-55 -0.053076641975579264 1101 56-57 0.17751883566724302 1101 58-59 0.07763428217227641 1101 60-61 0.30401649750378823 1101 62-63 0.7726238998160966 1101 64-65 0.2717963759508315 1101 66-67 0.4138879335024619 1101 68-69 0.4896160630493931 1101 70-71 0.19741252782947072 1101 72-73 0.4401574073166685 1101 74-75 0.6325958752467216 1101 76-77 0.8410024209951423 1101 78-79 0.4953598342750958 1101 80-81 0.4950473300740619 1101 82-83 0.8317317357958451 1101 84-85 0.7109145728031372 1101 86-87 0.3738324997841218 1101 88-89 0.03822073974820839 1101 90-91 0.39303767579630033 1101 92-93 0.6054339661264976 1101 94-95 0.8757786801855758 1101 96-97 0.08720244189865767 1101 98-99 0.07874882778684622 1101 100-101 0.1629201188265128 1102 1 -0.28042405222583966 1102 2 -0.11357751221157031 1102 3 -0.1907637973438696 1102 4 -0.020266092660378376 1102 5 -0.32202856069093855 1102 6 -0.025727634943237376 1102 7 -0.004120323567235573 1102 8 0.06799947940265127 1102 9 0.15657096010314575 1102 10-11 0.09418054368709505 1102 12-13 0.07224901106103232 1102 14-15 -0.00992429122031524 1102 16-17 -0.008260458641352386 1102 18-19 -0.0743650283548476 1102 20-21 0.003829565963968662 1102 22-23 -0.26695843632092675 1102 24-25 -0.3076244619943864 1102 26-27 0.028735343652122936 1102 28-29 -0.03098042136074497 1102 30-31 0.045049297446162484 1102 32-33 0.06127678457096408 1102 34-35 -0.169419554171931 1102 36-37 -0.39791320305378974 1102 38-39 -0.32164119217997467 1102 40-41 -0.3085616972405134 1102 42-43 -0.15705816471286482 1102 44-45 -0.14011442576665445 1102 46-47 -0.5269340657181516 1102 48-49 -0.27383844542222846 1102 50-51 -0.33384883472218974 1102 52-53 -0.6319127491880465 1102 54-55 -0.2516436538603273 1102 56-57 -0.3404140877930857 1102 58-59 -0.3198994265514301 1102 60-61 -0.4345660315615305 1102 62-63 -0.35703272378647455 1102 64-65 -0.32201203389082167 1102 66-67 -0.20112728979211525 1102 68-69 -0.4415969807866418 1102 70-71 -0.18888298041697027 1102 72-73 -0.38219438597567645 1102 74-75 -0.6154497828815764 1102 76-77 -0.7295067071038837 1102 78-79 -0.6560737416121434 1102 80-81 -0.5182363169335922 1102 82-83 -0.29528579156494317 1102 84-85 -0.7287153659510217 1102 86-87 -0.9165268640114732 1102 88-89 -0.7320779659778687 1102 90-91 -0.8509170892712348 1102 92-93 -0.45567668782862114 1102 94-95 -0.15975213669993593 1102 96-97 -0.504907306887592 1102 98-99 -0.9919718982291172 1102 100-101 -0.6501096106808184 1103 1 0.14505643356592657 1103 2 0.3385900780883162 1103 3 0.042325854033720134 1103 4 0.02544392581228294 1103 5 0.07751158787645096 1103 6 -0.13385167691942002 1103 7 -0.010408890167084905 1103 8 -0.03548674470614088 1103 9 0.13353908493119349 1103 10-11 0.18858764976734932 1103 12-13 -0.057727799849629946 1103 14-15 -0.013265092226482977 1103 16-17 0.010713325896631432 1103 18-19 0.0024537430164528473 1103 20-21 -0.09801573529767182 1103 22-23 -0.21711172013180402 1103 24-25 -0.16062921772287808 1103 26-27 -0.14380720243127598 1103 28-29 -0.07215088081914445 1103 30-31 -0.004030999688616532 1103 32-33 0.1896519761757247 1103 34-35 -0.11331374903888758 1103 36-37 -0.10327420608016524 1103 38-39 -0.2259763727797477 1103 40-41 0.17674860421765004 1103 42-43 0.10336709960345303 1103 44-45 0.09063666490661859 1103 46-47 -0.28322263231800093 1103 48-49 -0.24790793408315892 1103 50-51 -0.8004997799723625 1103 52-53 -0.5971684187238679 1103 54-55 0.0498636369465828 1103 56-57 -0.17120251683054022 1103 58-59 -0.3237511735938412 1103 60-61 -0.35558556542153497 1103 62-63 -0.09560521916831277 1103 64-65 0.17054977092778856 1103 66-67 0.25729938945168485 1103 68-69 0.6163364390144821 1103 70-71 0.6687497672935976 1103 72-73 0.38679905033293593 1103 74-75 0.4551492030870605 1103 76-77 -0.02850839715615905 1103 78-79 -0.04171026246267573 1103 80-81 0.12328590921898197 1103 82-83 0.23921289176642446 1103 84-85 0.3688458493144715 1103 86-87 -0.07940073894760857 1103 88-89 -0.061805199401600674 1103 90-91 -0.22067497945714365 1103 92-93 -0.3388960408923296 1103 94-95 -0.3651426173822472 1103 96-97 -0.05058784770432112 1103 98-99 -0.1608297373204195 1103 100-101 0.2998864589650587 1104 1 -0.2732051294324762 1104 2 -0.01215420686649793 1104 3 -0.14494155691789956 1104 4 -0.09271131076400252 1104 5 -0.6587324312623082 1104 6 -0.2548644360900738 1104 7 -0.2744971410974415 1104 8 -0.24249099776302785 1104 9 -0.09865123936439346 1104 10-11 -0.059451956824879915 1104 12-13 -0.16980249416116777 1104 14-15 -0.27157184395429823 1104 16-17 -0.36441958213100634 1104 18-19 -0.03293301934399295 1104 20-21 -0.21125337485110407 1104 22-23 -0.15112288440612076 1104 24-25 -0.2918426680152777 1104 26-27 -0.18660358733293236 1104 28-29 -0.5406184512604 1104 30-31 -0.35062190877952304 1104 32-33 -0.19770847039524142 1104 34-35 -0.17059710895382807 1104 36-37 -0.15298180841934794 1104 38-39 -0.162645697606969 1104 40-41 0.3669067647705404 1104 42-43 -0.14576899821685885 1104 44-45 -0.38205073541236345 1104 46-47 -0.08678456745888496 1104 48-49 -0.4428175566243624 1104 50-51 0.1460047389005794 1104 52-53 -0.15551504285997453 1104 54-55 0.04132429617040145 1104 56-57 0.4057844581827439 1104 58-59 0.3001855621849998 1104 60-61 0.26436392952795984 1104 62-63 0.19722439274024595 1104 64-65 -0.34204326469156143 1104 66-67 -0.16408551219637602 1104 68-69 -0.28973081244750887 1104 70-71 -0.17720982866599755 1104 72-73 -0.21912864770002827 1104 74-75 -0.3165812594328763 1104 76-77 0.5405373232053527 1104 78-79 0.2723514068621533 1104 80-81 0.6960530543545502 1104 82-83 0.6052748269073085 1104 84-85 0.17363718371106884 1104 86-87 0.3267661935351178 1104 88-89 0.07528943111408282 1104 90-91 0.3085647866247285 1104 92-93 0.3078011946206942 1104 94-95 0.6977893337076004 1104 96-97 0.1292699407061022 1104 98-99 -0.4677808271662478 1104 100-101 0.28749278538717604 1105 1 -0.14113885262935355 1105 2 0.032192771691008204 1105 3 -0.0401657284578647 1105 4 0.19968635005471214 1105 5 0.0690940794589423 1105 6 0.09089579782805401 1105 7 0.13437225461405689 1105 8 0.2986883394689457 1105 9 -0.11814441675230825 1105 10-11 0.26644960262930795 1105 12-13 0.06643044930861919 1105 14-15 -0.06250751646891217 1105 16-17 -0.1504819602986487 1105 18-19 -0.5303745398118238 1105 20-21 -0.14936253664447463 1105 22-23 -0.34337434639442677 1105 24-25 0.06622263412896956 1105 26-27 -0.04405972768380906 1105 28-29 0.012865954197692986 1105 30-31 -0.05201079766840877 1105 32-33 0.1799718414955862 1105 34-35 -0.5468154325405692 1105 36-37 -0.2467927245986843 1105 38-39 -0.10392250072587927 1105 40-41 -0.12249382002477915 1105 42-43 0.3617846080209617 1105 44-45 0.6491383484083002 1105 46-47 0.14859554950017895 1105 48-49 0.4309641197888965 1105 50-51 -0.007991362463940277 1105 52-53 0.7029157563602979 1105 54-55 1.0368164988994408 1105 56-57 1.1629725673445392 1105 58-59 0.39594579610312763 1105 60-61 0.6671417073057384 1105 62-63 0.7326776091145177 1105 64-65 0.4192871446651658 1105 66-67 -0.0368925297402356 1105 68-69 -0.18458948691144172 1105 70-71 0.3876049861488191 1105 72-73 0.7567485452824272 1105 74-75 1.064997687935545 1105 76-77 0.6823501887024257 1105 78-79 0.57571397996157 1105 80-81 0.48187176780484364 1105 82-83 0.38609841365194697 1105 84-85 -0.3323326018639783 1105 86-87 0.21857905903219077 1105 88-89 0.5606695480731467 1105 90-91 0.5108065020243373 1105 92-93 0.06303998604369454 1105 94-95 -0.1711190483586762 1105 96-97 -0.039224211340684434 1105 98-99 -0.10106542755611159 1105 100-101 0.6260649101435085 1106 1 0.17324529896409757 1106 2 0.3271970783232234 1106 3 0.4361477943672867 1106 4 0.3708749805456648 1106 5 0.29949890288702363 1106 6 0.3615676324533794 1106 7 0.34453590663817835 1106 8 0.5094979863251012 1106 9 0.451838356718838 1106 10-11 0.28119981796091764 1106 12-13 0.1531014226074987 1106 14-15 -0.06670648287717995 1106 16-17 0.30311407759970876 1106 18-19 0.5852585351235717 1106 20-21 0.30068052967765624 1106 22-23 0.5041708732438153 1106 24-25 0.24893150579133305 1106 26-27 0.10225810177356465 1106 28-29 0.4525731032071718 1106 30-31 0.46478506796466235 1106 32-33 0.4236195589805192 1106 34-35 0.5625730256850972 1106 36-37 0.6266999541265577 1106 38-39 0.8026235802388086 1106 40-41 1.1601289189933155 1106 42-43 1.4440412833008835 1106 44-45 1.5865845155056348 1106 46-47 1.2403268156500502 1106 48-49 0.9037246710378213 1106 50-51 1.6495237107488094 1106 52-53 0.5807150672991455 1106 54-55 1.2421351896832462 1106 56-57 1.305952756836355 1106 58-59 0.8294083284028702 1106 60-61 1.249829045807033 1106 62-63 0.7901711491661985 1106 64-65 1.3769744831664603 1106 66-67 1.82841754777915 1106 68-69 1.5362330798413524 1106 70-71 1.7472087759851682 1106 72-73 2.1395004832669287 1106 74-75 2.1144162925867107 1106 76-77 2.0024406279789133 1106 78-79 1.8874064864215185 1106 80-81 1.507711561086495 1106 82-83 1.9424076298448831 1106 84-85 2.1071592155348178 1106 86-87 1.8032043820296089 1106 88-89 2.230889186316041 1106 90-91 2.3058108086565525 1106 92-93 2.300227755208205 1106 94-95 2.7848456372571704 1106 96-97 1.7711763054551817 1106 98-99 1.8334737627970341 1106 100-101 1.7799193459746903 1107 1 0.7662685547780512 1107 2 0.8776954171936566 1107 3 0.36671257842044724 1107 4 0.19413079449915216 1107 5 0.24634275670761951 1107 6 -0.04640578947353191 1107 7 0.19204421228601376 1107 8 0.31747140825201114 1107 9 0.9641307155228205 1107 10-11 0.5880104491901577 1107 12-13 0.5178854757636486 1107 14-15 0.7116988327374401 1107 16-17 0.7477984629817698 1107 18-19 0.4656572062199231 1107 20-21 0.6342353469534103 1107 22-23 0.20583200281192404 1107 24-25 0.4266723695787107 1107 26-27 0.09046408184000398 1107 28-29 0.45440034573208266 1107 30-31 0.7440209483633353 1107 32-33 1.1099982965220505 1107 34-35 1.0346660489409132 1107 36-37 0.9665670637611115 1107 38-39 1.3386700918667174 1107 40-41 1.7207601482291892 1107 42-43 1.348027994264342 1107 44-45 1.8624981367680888 1107 46-47 0.7692304701350992 1107 48-49 1.4803027441275205 1107 50-51 1.8476964682238943 1107 52-53 1.204635333079878 1107 54-55 1.221869408952351 1107 56-57 0.9936603980323682 1107 58-59 1.3729299230872556 1107 60-61 1.353649643813668 1107 62-63 1.578709355146259 1107 64-65 1.2022236526016705 1107 66-67 1.128583660735952 1107 68-69 1.3599872326652758 1107 70-71 2.0942187427625747 1107 72-73 2.0293675929014796 1107 74-75 1.7899976879355464 1107 76-77 1.2057628871151245 1107 78-79 1.8189679482155405 1107 80-81 1.3477447836778573 1107 82-83 1.7653312178847536 1107 84-85 1.1269266573952805 1107 86-87 1.9741346145877472 1107 88-89 1.556304468708067 1107 90-91 1.306309147526985 1107 92-93 1.4176696156733257 1107 94-95 0.767902115662487 1107 96-97 0.6175218204053472 1107 98-99 0.630151503660823 1107 100-101 1.4473612064398047 1108 1 0.07952156682624434 1108 2 0.36530299034339464 1108 3 -0.14636831658815908 1108 4 0.17123922823409288 1108 5 0.14049077736166282 1108 6 -0.04330768276096819 1108 7 -0.01466835225787122 1108 8 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-2.731859572780973 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 3.0 6 9.0 7 23.0 8 56.0 9 91.0 10 156.0 11 245.0 12 433.0 13 644.0 14 843.0 15 1194.0 16 1755.0 17 2616.0 18 2353.0 19 1326.0 20 1073.0 21 1022.0 22 1028.0 23 1063.0 24 1178.0 25 1325.0 26 1463.0 27 1751.0 28 2004.0 29 2473.0 30 2936.0 31 3680.0 32 4715.0 33 6401.0 34 9045.0 35 13336.0 36 21197.0 37 31042.0 38 20905.0 39 1187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1192793715847 20.49038023679417 15.733151183970856 19.657189207650273 2 15.24277024863942 22.550421513178957 40.4510368868495 21.755771351332122 3 19.55411384751092 27.75691095081594 31.45389616856602 21.23507903310712 4 12.378533726009078 18.663479733094313 38.61738301535135 30.340603525545262 5 12.814785305750789 39.20339754716444 33.540107560538374 14.441709586546397 6 28.855976211817264 38.22755274801884 17.694595017570816 15.221876022593083 7 25.049796545542495 36.678712688159806 21.41393734171812 16.857553424579574 8 26.027572666353667 34.87344743693713 19.375560203167016 19.72341969354219 9 27.43679485537866 17.000298775022408 21.198087839856587 34.364818529742344 10-11 23.543827450310868 28.672442983766555 26.68167655469717 21.102053011225404 12-13 26.23883888869126 26.368325566504215 27.664259542527837 19.72857600227669 14-15 23.6491557556938 26.517487790736965 27.80652986600934 22.02682658755989 16-17 20.12605460469219 30.380795879750167 28.37971459871669 21.11343491684095 18-19 19.939462058859096 27.877686326677242 33.03016937818784 19.152682236275815 20-21 21.243206328429558 26.991506132088894 32.901644708761346 18.863642830720202 22-23 20.921025954763234 27.01329928186012 32.526507862546815 19.539166900829827 24-25 20.49347674534409 27.028824675971375 33.134505669611734 19.3431929090728 26-27 20.43087626445858 27.048031641697612 33.07652908788254 19.444563005961275 28-29 20.165607315745856 26.74010393139532 34.02099257683703 19.073296176021795 30-31 20.446170700129468 27.43679485537866 33.16367180275158 18.95336264174029 32-33 19.956037389559945 28.019050464524025 32.628010869719866 19.396901276196168 34-35 19.89983923058318 27.492637329804943 32.98582952750864 19.621693912103233 36-37 20.80784224454372 27.657001394303276 32.426799078052525 19.108357283100474 38-39 20.618241320432556 27.186966420034146 32.99978656801366 19.195005691519636 40-41 20.2565908347205 27.85223441034615 32.81901347333077 19.072161281602572 42-43 20.449016176533355 28.344501828218586 32.06887475635608 19.13760723889197 44-45 20.413092036934284 28.37651343776232 31.685091126381838 19.525303398921565 46-47 20.316701523752613 28.781993825316203 30.55508130948824 20.34622334144294 48-49 20.690170301762773 28.98544538819412 30.160982827549905 20.163401482493207 50-51 21.498973817222087 29.243897146962887 29.607062698522796 19.65006633729223 52-53 20.771695945537388 29.574919881770036 29.780150597374327 19.87323357531825 54-55 20.612631069756855 29.380255239233428 29.901333105695223 20.105780585314495 56-57 20.8530738258853 29.72918178326006 29.36104827350719 20.056696117347446 58-59 20.489071475573102 29.129452773479876 29.75937683401803 20.622098916928987 60-61 20.248918672813158 29.249544704342384 29.798745091343694 20.70279153150077 62-63 21.014198927255396 28.912885739895 29.868254442500035 20.204660890349565 64-65 20.94171376539675 28.681081427153764 30.398688230712047 19.978516576737437 66-67 20.37787926643618 29.07187673396218 30.099805084866333 20.4504389147353 68-69 20.45655831291104 29.290726915101352 29.915311207304363 20.337403564683243 70-71 20.085649449040712 29.568267021405248 29.52131632674838 20.82476720280566 72-73 20.17599396754711 28.87503290105497 29.41247608004382 21.5364970513541 74-75 20.554318519730526 28.721837674024858 29.37062937062937 21.35321443561525 76-77 20.419138674292544 28.76776644329677 29.494785664489875 21.31830921792081 78-79 20.2582297391026 27.88987888811823 29.82197798289139 22.02991338988778 80-81 20.226509589664786 29.167378066131693 29.050352854134655 21.555759490068862 82-83 19.935834278864792 29.492400772549594 28.885956456942452 21.68580849164316 84-85 19.888239144044164 28.909140060326678 28.493682772750557 22.70893802287861 86-87 19.92815095413399 28.843479343399313 28.38499706557115 22.843372636895552 88-89 20.294097769367305 27.922214199074453 28.95056041489555 22.833127616662694 90-91 19.75599345521804 27.86476488582201 29.651063527068366 22.728178131891585 92-93 19.72946633090045 28.23779646307283 28.82752144777839 23.205215758248325 94-95 19.199140666126027 29.083614324128217 28.709434177017084 23.00781083272867 96-97 19.161642568781232 29.538850059577797 28.490280815949063 22.809226555691904 98-99 19.29885715708488 30.323218598496837 27.074315023422415 23.30360922099587 100-101 19.30695710632183 30.917666475775157 26.086569892970328 23.688806524932684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 259.0 1 186.0 2 109.0 3 109.0 4 108.0 5 115.5 6 163.0 7 281.5 8 440.0 9 518.0 10 465.5 11 440.5 12 475.0 13 488.5 14 486.5 15 496.5 16 522.0 17 530.5 18 534.0 19 565.5 20 579.5 21 624.0 22 731.0 23 815.0 24 901.5 25 1033.5 26 1228.5 27 1457.0 28 1658.5 29 1909.5 30 2237.0 31 2532.5 32 2804.5 33 3085.0 34 3352.0 35 3642.5 36 4051.5 37 4257.0 38 4379.0 39 4455.0 40 4353.0 41 4401.5 42 4377.5 43 4503.0 44 4655.0 45 4574.0 46 4716.0 47 4984.0 48 6443.0 49 6305.0 50 4645.0 51 4213.0 52 3807.5 53 3600.0 54 3407.0 55 3061.5 56 2732.5 57 2456.0 58 2184.0 59 1912.0 60 1560.0 61 1259.0 62 997.5 63 717.0 64 488.0 65 343.5 66 262.0 67 202.0 68 151.0 69 108.5 70 85.5 71 60.5 72 32.5 73 17.5 74 18.0 75 15.5 76 8.5 77 4.5 78 3.5 79 2.5 80 3.0 81 3.0 82 1.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021341073029151905 2 0.006402321908745572 3 0.0 4 0.0 5 0.0014227382019434604 6 0.0 7 0.0014227382019434604 8 0.0 9 0.0 10-11 0.0 12-13 0.013516012918462873 14-15 0.003201160954372786 16-17 0.0 18-19 7.113691009717302E-4 20-21 0.0014227382019434604 22-23 0.0017784227524293254 24-25 0.0 26-27 0.0 28-29 3.556845504858651E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0014227382019434604 38-39 0.009959167413604222 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.003201160954372786 52-53 3.556845504858651E-4 54-55 0.0 56-57 0.0 58-59 0.0010670536514575954 60-61 0.004268214605830382 62-63 0.0 64-65 3.556845504858651E-4 66-67 0.0 68-69 3.556845504858651E-4 70-71 7.113691009717302E-4 72-73 7.113691009717302E-4 74-75 0.003556845504858651 76-77 0.0 78-79 0.0010670536514575954 80-81 0.004268214605830382 82-83 3.556845504858651E-4 84-85 0.004268214605830382 86-87 0.0010670536514575954 88-89 0.006046637358259707 90-91 0.002845476403886921 92-93 0.0 94-95 0.0 96-97 0.0010670536514575954 98-99 0.0024897918534010554 100-101 0.003912530055344515 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 140574.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.95600360792791 #Duplication Level Percentage of deduplicated Percentage of total 1 89.59936285626426 66.26410802665922 2 6.549161098927496 9.68699563722366 3 1.6489507672452215 3.658494266750493 4 0.7138513051551345 2.111743587983087 5 0.36891937546718334 1.3641901331542763 6 0.24694588666965203 1.0957878531302245 7 0.1689629693904354 0.8747078181691678 8 0.10597677349538526 0.6270094914385018 9 0.08198203231751444 0.5456757130073425 >10 0.4688972336177659 6.045317926176205 >50 0.027993864693596212 1.4972809198271007 >100 0.012997151464883956 2.290654957839189 >500 0.003999123527656602 1.8329676050624109 >1k 0.001999561763828301 2.1050660635791383 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1547 1.1004879992032666 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1300 0.9247798312632493 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 835 0.5939931993113947 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 563 0.4005008038470841 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 560 0.3983666965441689 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 521 0.37062330160627144 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 430 0.30588871341784396 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 329 0.23404043421969925 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.22763811231095366 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 304 0.21625620669540596 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 297 0.21127662298860383 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 277 0.1970492409691693 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.1650376314254414 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 188 0.13373739098268528 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 163 0.11595316345839202 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 159 0.1131076870545051 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 152 0.10812810334770298 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0014227382019434604 0.0 18-19 0.0 0.0 0.0 0.0014227382019434604 0.0 20-21 0.0 0.0 0.0 0.0014227382019434604 0.0 22-23 0.0 0.0 0.0 0.004623899156316246 0.0 24-25 0.0 0.0 0.0 0.009247798312632493 0.0 26-27 0.0 0.0 0.0 0.011737590166033549 0.0 28-29 0.0 0.0 0.0 0.022052442130123636 0.0 30-31 0.0 0.0 0.0 0.03485708594761478 0.0 32-33 0.0 0.0 0.0 0.04801741431559178 0.0 34-35 0.0 0.0 0.0 0.06437890363794159 0.0 36-37 0.0 0.0 0.0 0.08074039296029137 0.0 38-39 0.0 0.0 0.0 0.0988803050350705 0.0 40-41 0.0 0.0 0.0 0.10883947244867473 0.0 42-43 0.0 0.0 0.0 0.15756825586523826 0.0 44-45 0.0 0.0 0.0 0.21163230753908974 0.0 46-47 0.0 0.0 0.0 0.26036109095565324 0.0 48-49 0.0 0.0 0.0 0.3222502027401938 0.0 50-51 0.0 0.0 0.0 0.4012121729480558 0.0 52-53 0.0 0.0 0.0 0.5523781069045485 0.0 54-55 0.0 0.0 0.0 0.6679755858124546 0.0 56-57 0.0 0.0 0.0 0.9144649792991593 0.0 58-59 0.0 0.0 0.0 1.2345810747364379 0.0 60-61 0.0 0.0 0.0 1.5465164255125414 0.0 62-63 0.0 0.0 0.0 2.056568070909272 0.0 64-65 0.0 0.0 0.0 2.3240428528746424 0.0 66-67 0.0 0.0 0.0 2.8305376527665143 0.0 68-69 0.0 0.0 0.0 3.372245223156487 0.0 70-71 0.0 0.0 0.0 3.7997780528404967 0.0 72-73 0.0 0.0 0.0 4.6569778195114315 0.0 74-75 0.0 0.0 0.0 5.3925334699162 0.0 76-77 0.0 0.0 0.0 5.831092520665273 0.0 78-79 0.0 0.0 0.0 6.277476631525033 0.0 80-81 0.0 0.0 0.0 7.1474810420134585 0.0 82-83 0.0 0.0 0.0 7.6390370907849245 0.0 84-85 0.0 0.0 0.0 8.468137777967478 0.0 86-87 0.0 0.0 0.0 10.266123180673524 3.556845504858651E-4 88-89 0.0 0.0 0.0 12.531478082718 7.113691009717302E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTTTA 25 0.005009754 57.006832 9 ATCCATA 45 9.573753E-4 42.242317 2 GCTTATA 50 0.0016072288 38.01808 1 CCCCGTA 25 0.0016127889 38.004555 68-69 AATCCTT 80 1.0726322E-5 35.616592 5 AGGTAAG 55 0.0025659604 34.561897 2 GTAATCC 55 0.0025749383 34.537304 3 ACGCATT 30 0.0039367354 31.681736 34-35 TCTACGC 30 0.0039367354 31.681736 32-33 CGTATTA 30 0.003943603 31.670464 70-71 ACCGTTA 30 0.003943603 31.670464 78-79 ACCCCGT 30 0.003943603 31.670464 66-67 GGTAAGG 60 0.003943818 31.659197 3 CGAAATC 30 0.0039504804 31.659197 56-57 TATTCCG 30 0.0039504804 31.659197 52-53 ACGAAAT 30 0.0039504804 31.659197 54-55 TAATCCT 75 3.0690635E-4 31.659195 4 CTGAACT 85 6.374542E-4 27.944525 9 CGCATTC 35 0.008361542 27.155775 36-37 TACGCAT 35 0.008361542 27.155775 34-35 >>END_MODULE