FastQCFastQC Report
Thu 26 May 2016
SRR936923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909620
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT54090.5946439172401662No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51320.5641916404652493No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT42070.4625008245201293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38030.4180866735559904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28120.3091400804731646No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24610.27055253842263804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.267254457905499No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18230.20041335942481475No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18170.19975374332138696No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13810.15182163980563312No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12980.1426969503748818No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC11970.1315934126338471No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10840.11917064268595677No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10340.1136738418240584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.10773729689320816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA550.002573612434.5611239
TACTGCG650.005832068629.2424227
TACATGG45200.025.0265392
GTACATG44650.024.8025741
ACATGGG44500.024.2466683
GAGTACT38150.023.35336712-13
GCACCGT1553.8932798E-521.4601616
GTACTTT41200.021.33562314-15
AGTACTT40350.021.3146312-13
GTATAGG2901.05501385E-1021.3062821
AGAGTAC66150.020.43596610-11
CATGGGG16200.019.6583024
AGACGAC1507.698694E-419.0075747
GTATCAA113800.018.7110441
CATGGGA31550.018.6813624
GTGTAGC4600.018.598461
TATAGGG1801.2271265E-418.4836542
ACTTTTT48100.018.37530316-17
ACAACGT1300.00754458118.2765146
TACTTTT46800.018.17347714-15
Produced by FastQC (version 0.11.5)