##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936923_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909620 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86910797915613 33.0 31.0 34.0 30.0 34.0 2 31.967341307359117 33.0 31.0 34.0 30.0 34.0 3 31.98001583078648 34.0 31.0 34.0 30.0 34.0 4 35.53990567489721 37.0 35.0 37.0 33.0 37.0 5 35.563081286691144 37.0 35.0 37.0 33.0 37.0 6 35.64163276972802 37.0 35.0 37.0 33.0 37.0 7 35.58894373474638 37.0 35.0 37.0 33.0 37.0 8 35.559134583672304 37.0 35.0 37.0 33.0 37.0 9 37.19167454541457 39.0 37.0 39.0 34.0 39.0 10-11 37.07272982124404 39.0 37.0 39.0 33.0 39.0 12-13 36.984281348255315 39.0 37.0 39.0 33.0 39.0 14-15 38.130259888744746 40.0 38.0 41.0 33.0 41.0 16-17 38.06301587476089 40.0 38.0 41.0 33.0 41.0 18-19 38.053882940128844 40.0 38.0 41.0 33.0 41.0 20-21 38.00592884940964 40.0 38.0 41.0 33.0 41.0 22-23 37.874046305050456 40.0 38.0 41.0 33.0 41.0 24-25 37.68975836063411 40.0 37.0 41.0 32.5 41.0 26-27 37.63351344517491 40.0 37.0 41.0 32.5 41.0 28-29 37.71425430399508 40.0 37.5 41.0 33.0 41.0 30-31 37.57703436599898 40.0 37.0 41.0 32.5 41.0 32-33 37.358887777313605 40.0 37.0 41.0 32.0 41.0 34-35 37.135567599657 40.0 36.0 41.0 31.0 41.0 36-37 36.97028539390075 39.0 36.0 41.0 31.0 41.0 38-39 36.794663705723266 39.0 36.0 41.0 30.5 41.0 40-41 36.601862865812095 39.0 35.5 41.0 30.0 41.0 42-43 36.38526802401003 39.0 35.0 41.0 30.0 41.0 44-45 36.10589586860447 39.0 35.0 40.5 29.5 41.0 46-47 35.711724126558344 39.0 35.0 40.0 28.0 41.0 48-49 35.308526087816894 39.0 35.0 40.0 26.5 41.0 50-51 34.657167278643826 38.0 34.0 39.5 25.5 40.5 52-53 34.98687473890196 38.5 35.0 40.0 26.5 40.5 54-55 35.30697049317297 39.0 35.0 40.5 26.5 41.0 56-57 35.15516094632923 39.0 35.0 41.0 26.0 41.0 58-59 34.91165266814714 38.5 35.0 40.0 26.0 41.0 60-61 34.58461775246806 38.0 34.0 40.0 25.0 41.0 62-63 34.31515962709703 38.0 34.0 40.0 24.0 41.0 64-65 34.020820232624615 37.5 34.0 40.0 23.0 41.0 66-67 33.60976836481168 37.0 33.5 39.5 22.5 41.0 68-69 33.14734394582353 36.0 33.0 39.0 21.0 41.0 70-71 32.756260306501616 36.0 32.5 39.0 20.5 40.5 72-73 32.3797047118577 35.0 32.0 38.5 19.5 40.0 74-75 31.950343550053866 35.0 32.0 37.5 18.0 39.5 76-77 31.516302961676303 35.0 31.0 37.0 15.5 39.0 78-79 31.06158780589697 35.0 31.0 36.5 11.0 39.0 80-81 30.59188232448715 35.0 31.0 36.0 7.0 38.5 82-83 30.150503506958948 34.0 30.0 35.5 6.0 37.0 84-85 29.778782348673072 34.0 30.0 35.0 2.0 37.0 86-87 29.491523383390867 34.0 30.0 35.0 2.0 36.5 88-89 29.094736813174734 34.0 29.0 35.0 2.0 36.0 90-91 28.719569160748442 34.0 29.0 35.0 2.0 36.0 92-93 28.33750961940151 34.0 29.0 35.0 2.0 35.0 94-95 27.934760669290473 34.0 28.5 35.0 2.0 35.0 96-97 27.490192607902202 34.0 27.0 35.0 2.0 35.0 98-99 26.897873837426616 34.0 26.5 35.0 2.0 35.0 100-101 24.782772476418724 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3317378085252045 1101 2 0.21203886541663408 1101 3 0.19178037806683434 1101 4 0.21634851113666542 1101 5 0.2692656316743509 1101 6 0.21521060876412434 1101 7 0.17708687993395245 1101 8 0.13170112192054262 1101 9 0.26026015352876186 1101 10-11 0.17349780018999894 1101 12-13 0.2829570346163024 1101 14-15 0.46929525583665566 1101 16-17 0.5488369845156598 1101 18-19 0.40631860147769316 1101 20-21 0.45638426076827443 1101 22-23 0.5857646147601017 1101 24-25 0.6654243718360746 1101 26-27 0.6266237481284094 1101 28-29 0.5046745570463642 1101 30-31 0.37117292239189226 1101 32-33 0.4401263558772115 1101 34-35 0.41581070927167474 1101 36-37 0.5852441490145424 1101 38-39 0.5430520501359482 1101 40-41 0.5230570769110514 1101 42-43 0.5035082370537012 1101 44-45 0.5836381893507436 1101 46-47 0.6174782382341917 1101 48-49 0.5603645010240612 1101 50-51 0.6716396832766094 1101 52-53 0.5149872320806992 1101 54-55 0.7024804045823885 1101 56-57 0.8320237194324704 1101 58-59 0.8111269959034004 1101 60-61 0.8371815004362162 1101 62-63 0.803144601825089 1101 64-65 0.6591614980500182 1101 66-67 0.7615464924805408 1101 68-69 0.8558746173024758 1101 70-71 0.7455633490064102 1101 72-73 0.7826574717921844 1101 74-75 0.5253454471811629 1101 76-77 0.5343504742278249 1101 78-79 0.6196213485138458 1101 80-81 0.7636174151155153 1101 82-83 0.6394474641966106 1101 84-85 0.7871585557988681 1101 86-87 1.048651579020607 1101 88-89 1.0535955145052043 1101 90-91 0.9114834233148592 1101 92-93 1.1029411706370311 1101 94-95 1.282865870700526 1101 96-97 1.1457215258497726 1101 98-99 1.182440469585039 1101 100-101 1.5595103321913903 1102 1 0.38518911461964933 1102 2 0.39833444448332855 1102 3 0.4491666230577387 1102 4 0.3419936034631732 1102 5 0.25219554407106415 1102 6 0.38106707758590375 1102 7 0.24816014452373025 1102 8 0.3414947172351077 1102 9 0.2935451774431641 1102 10-11 0.42601167153944886 1102 12-13 0.4248232780162198 1102 14-15 0.574571464732216 1102 16-17 0.44731418130984224 1102 18-19 0.44380946577711455 1102 20-21 0.4203662951334266 1102 22-23 0.44761697438104875 1102 24-25 0.4430536513213923 1102 26-27 0.5024015256299705 1102 28-29 0.5835485912722973 1102 30-31 0.6114427798003845 1102 32-33 0.8964984138087431 1102 34-35 0.5896867073624961 1102 36-37 0.6111463460573106 1102 38-39 0.6201471086541517 1102 40-41 0.5753313070262962 1102 42-43 0.552174176178859 1102 44-45 0.6125473991312944 1102 46-47 0.4357309329551171 1102 48-49 0.5002444442459932 1102 50-51 0.518208388716765 1102 52-53 0.6669403454280172 1102 54-55 0.6836984607041785 1102 56-57 0.804458141260703 1102 58-59 0.7721744195115363 1102 60-61 0.7969393608675688 1102 62-63 0.9453346343407247 1102 64-65 1.094155705891552 1102 66-67 0.8272651178745534 1102 68-69 0.5861520691505007 1102 70-71 0.7638770105454853 1102 72-73 0.9161353397806948 1102 74-75 0.8378970297082411 1102 76-77 0.8920460900859091 1102 78-79 1.0589808053933396 1102 80-81 1.2064005845448484 1102 82-83 1.1414135042495097 1102 84-85 1.138899807899456 1102 86-87 1.1722986252683825 1102 88-89 1.0989676296125452 1102 90-91 0.846357375505935 1102 92-93 0.7425225566781783 1102 94-95 1.1091974456704463 1102 96-97 1.1154671307698756 1102 98-99 1.3219062610474452 1102 100-101 1.489489848334852 1103 1 0.18787491786764576 1103 2 0.16602696351136714 1103 3 0.16308711229213557 1103 4 0.3127799602542538 1103 5 0.2651819293533322 1103 6 0.06780392076226605 1103 7 0.1706793760756824 1103 8 0.04240083877037648 1103 9 0.10601719502629692 1103 10-11 0.17753035361849356 1103 12-13 0.03454233455087774 1103 14-15 0.08773083222784095 1103 16-17 0.19327072207259022 1103 18-19 0.26968633296747413 1103 20-21 0.16764055334498806 1103 22-23 0.15048059316895745 1103 24-25 0.19590658591376098 1103 26-27 0.34719450662228013 1103 28-29 0.29524320643775326 1103 30-31 0.3642804512562847 1103 32-33 0.33689968166660833 1103 34-35 0.46958871802634405 1103 36-37 0.41343912354112433 1103 38-39 0.4023764932444891 1103 40-41 0.5113711336370628 1103 42-43 0.5692816083087777 1103 44-45 0.6000901359134119 1103 46-47 0.47232557457667923 1103 48-49 0.3358413855134472 1103 50-51 0.22650949987309588 1103 52-53 0.2980341526647834 1103 54-55 0.29550729500756745 1103 56-57 0.23708411301372223 1103 58-59 0.2063712223932157 1103 60-61 0.1572854779256474 1103 62-63 0.16234590871086851 1103 64-65 0.37322434930398884 1103 66-67 0.2767288730131483 1103 68-69 0.3870062960217595 1103 70-71 0.2664463051850703 1103 72-73 0.2624953361175386 1103 74-75 0.2300070056942367 1103 76-77 0.0865289750222118 1103 78-79 0.27223480433509195 1103 80-81 0.3341479491099548 1103 82-83 0.33680073002182453 1103 84-85 0.285901619124985 1103 86-87 0.3862191145027829 1103 88-89 0.3613536648602178 1103 90-91 0.3241075383127239 1103 92-93 0.5315313177185139 1103 94-95 0.41983534302076464 1103 96-97 0.6718911305256654 1103 98-99 0.8806146435619979 1103 100-101 1.2963653825446322 1104 1 0.2818530828268919 1104 2 0.4236737144273981 1104 3 0.3791261461663602 1104 4 0.28140779245345726 1104 5 0.3597912167234014 1104 6 0.18929124596976976 1104 7 0.27327789548045445 1104 8 0.3335060507646972 1104 9 0.2924368465629783 1104 10-11 0.43573569273463164 1104 12-13 0.3890188917930857 1104 14-15 0.48559369022530063 1104 16-17 0.4789336249365661 1104 18-19 0.48264041955986414 1104 20-21 0.4967678994902087 1104 22-23 0.5758952346106625 1104 24-25 0.576415073164199 1104 26-27 0.4806967125596202 1104 28-29 0.5142830628648838 1104 30-31 0.4073814757085401 1104 32-33 0.4571442042120424 1104 34-35 0.5661769929085736 1104 36-37 0.6148146563310775 1104 38-39 0.5024561313212104 1104 40-41 0.5794680453247949 1104 42-43 0.5442546883955188 1104 44-45 0.49099382244597933 1104 46-47 0.4281488752571505 1104 48-49 0.30006818578264927 1104 50-51 0.15850718176945833 1104 52-53 0.4708125120456259 1104 54-55 0.37337501354492275 1104 56-57 0.3022157214785466 1104 58-59 0.3551332802710263 1104 60-61 0.25434111241305146 1104 62-63 0.3878147638667784 1104 64-65 0.18550469028955519 1104 66-67 0.195038152905461 1104 68-69 0.3768563670604195 1104 70-71 0.15092734907948824 1104 72-73 0.2694040327450722 1104 74-75 0.2759821047744744 1104 76-77 0.36253622844328603 1104 78-79 0.3193495130765882 1104 80-81 0.3444679969675768 1104 82-83 0.4019327123743288 1104 84-85 0.4143406380437007 1104 86-87 0.2834209390746807 1104 88-89 0.4435562372074813 1104 90-91 0.4454135279934235 1104 92-93 0.6071078525274558 1104 94-95 0.6629853467596405 1104 96-97 0.6975222529364906 1104 98-99 0.7389657988749434 1104 100-101 0.6761339460681484 1105 1 0.18888612084830214 1105 2 0.30480420538346564 1105 3 0.27843124029704214 1105 4 0.267613957089857 1105 5 0.188489998561721 1105 6 0.08230527328612425 1105 7 0.17639251342065165 1105 8 0.2735069736144595 1105 9 0.2710793345659326 1105 10-11 0.3323432970166422 1105 12-13 0.4221233583859174 1105 14-15 0.4631024572980209 1105 16-17 0.5001070284064824 1105 18-19 0.5109746757429647 1105 20-21 0.3363360058025009 1105 22-23 0.29239671117027655 1105 24-25 0.4983392149139192 1105 26-27 0.40181382219417117 1105 28-29 0.49570702004465517 1105 30-31 0.17670548825929444 1105 32-33 0.3982816165413112 1105 34-35 0.2067062472459682 1105 36-37 0.06696812624617365 1105 38-39 0.1936539903097696 1105 40-41 0.20424932993166323 1105 42-43 0.4265839611899125 1105 44-45 0.3791550442990612 1105 46-47 0.5436712082213475 1105 48-49 0.5649043799502351 1105 50-51 0.38836737003080657 1105 52-53 0.5544729956189016 1105 54-55 0.5555778559221807 1105 56-57 0.595321110921148 1105 58-59 0.4395380421757906 1105 60-61 0.36595056239459467 1105 62-63 0.43106273359730807 1105 64-65 0.47090751420982 1105 66-67 0.5977028815876295 1105 68-69 0.6009953482342851 1105 70-71 0.5559518045981306 1105 72-73 0.37472610731133216 1105 74-75 0.43377557099817565 1105 76-77 0.27963525022253677 1105 78-79 0.2731534997875542 1105 80-81 0.2111097466907239 1105 82-83 0.5706693310528053 1105 84-85 0.43268873697319776 1105 86-87 0.6315235599679987 1105 88-89 0.6803419514931974 1105 90-91 0.6503369314633396 1105 92-93 0.8283800009216975 1105 94-95 0.854682928893272 1105 96-97 0.8015292665946703 1105 98-99 0.8680623289598444 1105 100-101 1.071077652232784 1106 1 0.33599776524255276 1106 2 0.2639335422301947 1106 3 0.3911382796345144 1106 4 0.15868695414111755 1106 5 0.20333566014651439 1106 6 0.17631211185818074 1106 7 0.3977010961422778 1106 8 0.37794028185211204 1106 9 0.3374299525469837 1106 10-11 0.3445931244838647 1106 12-13 0.42582497078318227 1106 14-15 0.6728572254766974 1106 16-17 0.44153803097879063 1106 18-19 0.4551642422151332 1106 20-21 0.5201775266005413 1106 22-23 0.5800598330725322 1106 24-25 0.44797924879308226 1106 26-27 0.5927076204249246 1106 28-29 0.5651553856886693 1106 30-31 0.49024264554645214 1106 32-33 0.6989611604273733 1106 34-35 0.7187737389330167 1106 36-37 0.5730283401868235 1106 38-39 0.6179826809428022 1106 40-41 0.36873973083510947 1106 42-43 0.5314054681853335 1106 44-45 0.68972777470875 1106 46-47 0.2884638551879064 1106 48-49 0.3684071875291295 1106 50-51 0.33088454488493824 1106 52-53 0.3856986841815484 1106 54-55 0.4459215098527949 1106 56-57 0.6764468088946671 1106 58-59 0.7633546241445472 1106 60-61 0.7278772830169657 1106 62-63 0.8315877822201827 1106 64-65 0.7712968893274521 1106 66-67 0.8909752794795978 1106 68-69 1.0298373395762752 1106 70-71 1.0004766336338307 1106 72-73 1.055316953663045 1106 74-75 1.0565287236426322 1106 76-77 1.3159945732666394 1106 78-79 1.208053744470405 1106 80-81 1.2972075257232802 1106 82-83 1.3211673233986225 1106 84-85 1.2342211417664686 1106 86-87 1.1613717311205178 1106 88-89 1.1097485955737554 1106 90-91 0.9766081843650269 1106 92-93 0.9296822313000135 1106 94-95 1.0737191764621272 1106 96-97 1.071111737264097 1106 98-99 0.9173792559941631 1106 100-101 0.9363177864946586 1107 1 0.01796725271211841 1107 2 0.25398461370698655 1107 3 0.27417202777811056 1107 4 0.22586666335492822 1107 5 0.1605790108450762 1107 6 0.18111199267588063 1107 7 0.21016123292258726 1107 8 0.3096804830653497 1107 9 0.3036631867169248 1107 10-11 0.3320207887436126 1107 12-13 0.33154059995864316 1107 14-15 0.30888566162804665 1107 16-17 0.16934149192098147 1107 18-19 0.2981788094576956 1107 20-21 0.35825254627028613 1107 22-23 0.4607425241715717 1107 24-25 0.3523796826911365 1107 26-27 0.48166091120645405 1107 28-29 0.3202660250365241 1107 30-31 0.560646329024344 1107 32-33 0.47771505363817823 1107 34-35 0.6704117971926777 1107 36-37 0.6333294617108649 1107 38-39 0.5859151939667271 1107 40-41 0.41661051115326586 1107 42-43 0.5347644536460265 1107 44-45 0.6779957672784747 1107 46-47 0.6128422217386529 1107 48-49 0.6672218823339904 1107 50-51 0.7490700875135587 1107 52-53 0.6435886919563245 1107 54-55 0.5454433839944244 1107 56-57 0.5179436640648944 1107 58-59 0.7164289222027165 1107 60-61 0.8185698904556205 1107 62-63 0.6462616370691805 1107 64-65 0.7613805497137704 1107 66-67 0.7816325046736168 1107 68-69 0.7685138574047343 1107 70-71 0.845085729664028 1107 72-73 0.9196283732825066 1107 74-75 0.9211241323308279 1107 76-77 0.8134968661292099 1107 78-79 0.9495585140900928 1107 80-81 0.848677052765094 1107 82-83 0.7844116890089445 1107 84-85 0.8864648881875112 1107 86-87 1.0205666919439942 1107 88-89 0.8269955776079527 1107 90-91 0.7267047872039853 1107 92-93 0.7527500581318627 1107 94-95 0.5613423741790413 1107 96-97 0.6813949348879014 1107 98-99 1.0510752573349613 1107 100-101 1.1355477571180863 1108 1 -0.09387242930715445 1108 2 0.029532154982550907 1108 3 -0.04374393561525025 1108 4 0.019219668102785192 1108 5 0.028634819376641474 1108 6 0.01781091884331687 1108 7 -0.05218096064250943 1108 8 -0.13905848212110072 1108 9 -0.24146975723574116 1108 10-11 -0.01804943739670506 1108 12-13 -0.12475931220809144 1108 14-15 -0.04010385099849856 1108 16-17 -0.22377715585141544 1108 18-19 0.11573453501312514 1108 20-21 -0.05153013721828614 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-2.774185690163602 2316 84-85 -2.1041453035737163 2316 86-87 -2.1645239019210436 2316 88-89 -2.3357176901852554 2316 90-91 -2.4595267334745827 2316 92-93 -2.8107200697996575 2316 94-95 -3.0557415957020453 2316 96-97 -2.815337777252573 2316 98-99 -2.963060961940677 2316 100-101 -2.9805391165869572 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2459.0 3 694.0 4 684.0 5 759.0 6 751.0 7 901.0 8 1116.0 9 1381.0 10 1716.0 11 2319.0 12 2956.0 13 3681.0 14 4332.0 15 5523.0 16 7021.0 17 9613.0 18 8536.0 19 5378.0 20 5082.0 21 5330.0 22 5559.0 23 6125.0 24 7029.0 25 7937.0 26 9215.0 27 11036.0 28 13078.0 29 15783.0 30 19403.0 31 24349.0 32 31441.0 33 41501.0 34 57711.0 35 83918.0 36 125624.0 37 181704.0 38 165821.0 39 32141.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.531201872728396 18.908462479429026 14.93210479534634 21.628230852496237 2 15.049290520241138 20.71093239308282 40.70710272104801 23.532674365628033 3 20.00085810846491 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21.515474403327406 26.127457748698617 30.61357971651485 21.74348813145913 24-25 21.959923457334725 26.207502347268065 30.439460285104374 21.393113910292833 26-27 21.41058253148057 26.016329471423425 31.210135382263083 21.362952614832924 28-29 20.874398157494202 27.179435839862176 30.667688325204256 21.27847767743937 30-31 21.183934090834093 26.28529473232651 30.592599518220716 21.93817165861868 32-33 21.27686370613414 26.40302832482446 30.664593666938167 21.655514302103228 34-35 22.040608690391732 26.319864931008123 30.255509176002153 21.384017202597988 36-37 21.681591870159252 25.943125652742772 30.986603339733122 21.388679137364853 38-39 21.394123551168004 26.615995930208573 30.641679613919788 21.348200904703635 40-41 21.356333093141327 26.799212316899435 30.436856088317317 21.40759850164192 42-43 21.116140256502916 26.88143299291721 29.661800514721936 22.340626235857936 44-45 21.454926225816262 26.470327813766453 29.82562465661966 22.24912130379763 46-47 22.12817458877301 26.966119757041472 29.43507633969763 21.470629314487883 48-49 21.802364907595706 26.96546786169547 29.282256813874884 21.949910416833937 50-51 21.168551039158622 27.279240305465734 29.79539030235917 21.75681835301647 52-53 21.75612524457535 27.765117478654993 28.573109744629793 21.90564753213987 54-55 21.297172146023296 27.080578989627902 29.105667806900797 22.516581057448 56-57 21.896861608046393 27.20606999234431 28.61401254828803 22.283055851321265 58-59 22.485822197068572 26.983723645731754 28.595573874452125 21.93488028274755 60-61 21.894466474900863 27.07758815085332 29.703716333276496 21.324229040969318 62-63 21.335359588852736 27.304783151058338 29.54983466546983 21.81002259461909 64-65 21.193754406108777 27.904732367041372 28.976111054287664 21.92540217256219 66-67 21.546661386269317 27.317915673154403 28.472177531359232 22.663245409217044 68-69 21.28051936195126 27.368689676944243 28.955946741640638 22.394844219463863 70-71 22.037947394436163 27.16863657581644 28.570069733519322 22.223346296228076 72-73 21.704865900867638 26.83890455515353 29.303990838121642 22.15223870585719 74-75 21.182385731928196 28.198642738452772 28.52107758341022 22.097893946208806 76-77 21.458181186263207 27.3880674169167 28.388333582634555 22.765417814185533 78-79 21.68302928329924 27.406140697785062 28.57523952637609 22.335590492539602 80-81 22.041670584728852 27.511395374541998 28.18821655858314 22.25871748214601 82-83 21.618742172473176 27.267432043179074 29.02866339255807 22.085162391789684 84-85 21.62420231519729 27.577370338061048 28.488169348442362 22.3102579982993 86-87 21.397749093383307 27.742333292813125 28.505799981628392 22.35411763217517 88-89 21.435350260476124 27.49449421307822 27.998996497092648 23.071159029353012 90-91 21.53592290683476 27.09806057005819 28.444825803328822 22.921190719778224 92-93 21.932059728291723 27.36501502889156 28.556159205031044 22.14676603778567 94-95 21.470041156927223 27.216826751947675 28.622467027970316 22.690665063154785 96-97 20.764566712517194 27.40302613480055 29.287647867950483 22.544759284731775 98-99 21.04678310432167 27.873928554524152 28.198100320429205 22.881188020724977 100-101 20.98007662919592 27.386902593307216 28.42396131777562 23.209059459721242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 201.0 2 166.0 3 206.0 4 205.5 5 205.5 6 355.0 7 729.5 8 1225.5 9 1499.0 10 1402.5 11 1253.0 12 1248.5 13 1300.5 14 1432.0 15 1498.0 16 1523.5 17 1628.5 18 1729.0 19 1876.5 20 2100.0 21 2418.5 22 2827.5 23 3300.5 24 3892.5 25 4782.5 26 5959.5 27 7314.0 28 8593.0 29 9901.5 30 11618.5 31 13620.0 32 15630.0 33 17588.5 34 19686.0 35 21332.5 36 23506.0 37 25645.5 38 26374.0 39 26834.5 40 27443.5 41 28509.5 42 29075.0 43 29598.0 44 31063.0 45 32149.5 46 32812.5 47 37110.0 48 46436.5 49 45571.5 50 35611.5 51 30345.5 52 28151.5 53 27174.0 54 25886.5 55 23623.5 56 21237.0 57 18962.0 58 17119.0 59 15171.0 60 12754.0 61 10461.0 62 8232.0 63 6239.5 64 4544.5 65 3231.0 66 2304.5 67 1756.0 68 1291.5 69 874.5 70 610.0 71 430.5 72 309.0 73 243.5 74 174.0 75 118.5 76 78.5 77 46.0 78 30.5 79 21.0 80 14.0 81 10.0 82 11.0 83 11.0 84 8.5 85 6.0 86 5.5 87 4.0 88 4.5 89 5.0 90 1.5 91 1.0 92 1.0 93 0.0 94 1.0 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06288340186011741 2 0.1018007519623579 3 0.07079879510125107 4 0.1335722609441305 5 0.10872672104834985 6 0.10454915239330709 7 0.11312416173786856 8 0.09729337525560125 9 0.11554275411710384 10-11 0.10850684901387393 12-13 0.09113695829027506 14-15 0.09652382313493547 16-17 0.11021085728106243 18-19 0.08943295002308657 20-21 0.07327235548910534 22-23 0.07514126778215079 24-25 0.064257602075592 26-27 0.05799124909302786 28-29 0.07041401904091818 30-31 0.05386864844660408 32-33 0.05172489611046371 34-35 0.050020887843275214 36-37 0.052274576196653544 38-39 0.05304412831731932 40-41 0.0687100107737297 42-43 0.06436753809282998 44-45 0.05062553593808403 46-47 0.036168949671291306 48-49 0.04496383105032871 50-51 0.02797871638706273 52-53 0.042984982740045294 54-55 0.04947120775708537 56-57 0.05408852048108001 58-59 0.05942041731712143 60-61 0.05771640904993294 62-63 0.060904553549834 64-65 0.04078626239528594 66-67 0.06568677029968559 68-69 0.055462720696554606 70-71 0.025230315956113542 72-73 0.021932235438974514 74-75 0.03177150898177261 76-77 0.045293639102042614 78-79 0.06447747411006793 80-81 0.040621358369429 82-83 0.06249862579978452 84-85 0.06409269804973505 86-87 0.06689606648930323 88-89 0.08833358985070688 90-91 0.06486225017040083 92-93 0.07739495613552913 94-95 0.0859149974714716 96-97 0.09564433499703172 98-99 0.1265363558409006 100-101 0.1197203227721466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 909620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.03621425117221 #Duplication Level Percentage of deduplicated Percentage of total 1 85.57831518388944 59.080029022893356 2 9.2351712064803 12.751225161136611 3 2.2796230332974994 4.721296324158997 4 0.9641866175970144 2.662551756421621 5 0.49482516935632087 1.708042820427777 6 0.3136836190441975 1.299331771885098 7 0.2095028433484357 1.0124298225742663 8 0.14309580961338547 0.7903034376731702 9 0.11475769335551608 0.7130193034915556 >10 0.6181102931147251 7.489752901107552 >50 0.033073842807591146 1.5495999973375572 >100 0.011820887171875393 1.4789601355324014 >500 0.0015974170515484234 0.7856320543357868 >1k 0.001916900461858108 2.795367955264391 >5k 3.194834103096847E-4 1.1624575357599 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5409 0.5946439172401662 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5132 0.5641916404652493 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4207 0.4625008245201293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3803 0.4180866735559904 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2812 0.3091400804731646 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2461 0.27055253842263804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2431 0.267254457905499 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1823 0.20041335942481475 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1817 0.19975374332138696 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1381 0.15182163980563312 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1298 0.1426969503748818 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1197 0.1315934126338471 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1084 0.11917064268595677 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1034 0.1136738418240584 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.10773729689320816 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 10-11 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 12-13 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 14-15 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 16-17 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 18-19 1.0993601723796751E-4 0.0 0.0 1.0993601723796751E-4 0.0 20-21 1.0993601723796751E-4 0.0 0.0 1.6490402585695127E-4 0.0 22-23 1.0993601723796751E-4 0.0 0.0 2.1987203447593502E-4 0.0 24-25 1.0993601723796751E-4 0.0 0.0 3.8477606033288623E-4 0.0 26-27 1.0993601723796751E-4 0.0 0.0 7.145841120467888E-4 0.0 28-29 1.0993601723796751E-4 0.0 0.0 0.001978848310283415 0.0 30-31 1.0993601723796751E-4 0.0 0.0 0.0043974406895187 0.0 32-33 1.0993601723796751E-4 0.0 0.0 0.0075306171808007735 0.0 34-35 1.0993601723796751E-4 0.0 0.0 0.011158505749653703 0.0 36-37 1.0993601723796751E-4 0.0 0.0 0.01451155427541171 0.0 38-39 1.0993601723796751E-4 0.0 0.0 0.017479826740836833 0.0 40-41 1.0993601723796751E-4 0.0 0.0 0.02138255535278468 0.0 42-43 1.0993601723796751E-4 0.0 0.0 0.02885820452496647 0.0 44-45 1.0993601723796751E-4 0.0 0.0 0.035949077636815374 0.0 46-47 1.0993601723796751E-4 0.0 0.0 0.05843099316197973 0.0 48-49 1.0993601723796751E-4 0.0 0.0 0.10493392845363998 0.0 50-51 1.0993601723796751E-4 0.0 0.0 0.13423187704755832 0.0 52-53 1.0993601723796751E-4 0.0 0.0 0.1606165211846705 0.0 54-55 1.0993601723796751E-4 0.0 0.0 0.20222730370924122 0.0 56-57 1.0993601723796751E-4 0.0 0.0 0.23388887667377584 0.0 58-59 1.0993601723796751E-4 0.0 0.0 0.2719817066467316 0.0 60-61 1.0993601723796751E-4 0.0 0.0 0.33546975660165784 0.0 62-63 1.0993601723796751E-4 0.0 0.0 0.3818627558760801 0.0 64-65 1.0993601723796751E-4 0.0 0.0 0.43595127635716013 0.0 66-67 1.0993601723796751E-4 0.0 0.0 0.5352234999230447 0.0 68-69 1.0993601723796751E-4 0.0 0.0 0.6436753809282998 0.0 70-71 1.0993601723796751E-4 0.0 0.0 0.7351421472702887 0.0 72-73 1.0993601723796751E-4 0.0 0.0 0.8456278445944461 0.0 74-75 1.0993601723796751E-4 0.0 0.0 0.9346210505485808 0.0 76-77 1.0993601723796751E-4 0.0 0.0 0.9984389085552208 0.0 78-79 1.0993601723796751E-4 0.0 0.0 1.0930388513884919 0.0 80-81 1.0993601723796751E-4 0.0 0.0 1.2260614322464325 0.0 82-83 1.0993601723796751E-4 0.0 0.0 1.3817308326553945 0.0 84-85 1.0993601723796751E-4 0.0 0.0 1.556584068072382 0.0 86-87 1.0993601723796751E-4 0.0 0.0 1.8861722477518086 0.0 88-89 1.0993601723796751E-4 0.0 0.0 2.310415338273125 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTCGCA 55 0.0025736124 34.561123 9 TACTGCG 65 0.0058320686 29.242422 7 TACATGG 4520 0.0 25.026539 2 GTACATG 4465 0.0 24.802574 1 ACATGGG 4450 0.0 24.246668 3 GAGTACT 3815 0.0 23.353367 12-13 GCACCGT 155 3.8932798E-5 21.460161 6 GTACTTT 4120 0.0 21.335623 14-15 AGTACTT 4035 0.0 21.31463 12-13 GTATAGG 290 1.05501385E-10 21.306282 1 AGAGTAC 6615 0.0 20.435966 10-11 CATGGGG 1620 0.0 19.658302 4 AGACGAC 150 7.698694E-4 19.007574 7 GTATCAA 11380 0.0 18.711044 1 CATGGGA 3155 0.0 18.681362 4 GTGTAGC 460 0.0 18.59846 1 TATAGGG 180 1.2271265E-4 18.483654 2 ACTTTTT 4810 0.0 18.375303 16-17 ACAACGT 130 0.007544581 18.276514 6 TACTTTT 4680 0.0 18.173477 14-15 >>END_MODULE