FastQCFastQC Report
Thu 26 May 2016
SRR936923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences909620
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT78510.8631076713352828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.7098568633055562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52700.5793628108440888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33780.37136386622985423No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT27540.30276379147336246No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT23560.2590092566126514No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19980.21965216244145908No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC19010.2089883687693762No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18590.20437105604538158No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17780.1954662386491062No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT17330.1905191178733977No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16080.17677711571865176No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.16534376992590313No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14930.16413447373628548No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12050.13247290077175083No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9440.10377960027264131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGACG700.00839343727.141028
GAGTACT56900.024.33271212-13
GTACTTT60000.022.91845714-15
AGTACTT58700.022.37284512-13
GTACATG46600.021.9185711
GTATAGG3101.0913936E-1121.4548781
AGAGTAC88200.020.65194710-11
TACATGG48000.020.5819422
ACTTTTT68100.020.19358416-17
GTGTAGC4250.020.1207081
TACTTTT65800.020.0320414-15
ACATGGG46500.020.0201513
GTATAAT5700.018.3361231
CTATACC1851.5216839E-417.9717564
CTATACA4253.6379788E-1217.8811424
CATGGGG17000.017.3223554
ATGGGAG12400.017.2367345
CATGGGA29200.017.0793254
CGCGATC850.001788256316.76449222-23
GTATCAA160200.016.6957361
Produced by FastQC (version 0.11.5)