##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936923_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 909620 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.309667773355905 33.0 31.0 34.0 31.0 34.0 2 32.310873771465005 33.0 31.0 34.0 31.0 34.0 3 32.479318836437194 34.0 31.0 34.0 31.0 34.0 4 36.16973571381456 37.0 35.0 37.0 35.0 37.0 5 36.12755766144104 37.0 35.0 37.0 35.0 37.0 6 36.19208350739869 37.0 36.0 37.0 35.0 37.0 7 36.150638728260155 37.0 36.0 37.0 35.0 37.0 8 36.134719992964094 37.0 36.0 37.0 35.0 37.0 9 37.851458850948745 39.0 38.0 39.0 35.0 39.0 10-11 37.78077493898551 39.0 37.0 39.0 35.0 39.0 12-13 37.699381609903035 39.0 37.0 39.0 35.0 39.0 14-15 38.907535564301575 40.0 38.0 41.0 35.5 41.0 16-17 38.85097293375256 40.0 38.0 41.0 35.5 41.0 18-19 38.947357687825686 40.0 38.0 41.0 35.5 41.0 20-21 39.03464193839186 40.0 38.0 41.0 36.0 41.0 22-23 38.96617488621622 40.0 38.0 41.0 35.0 41.0 24-25 38.85407862623953 40.0 38.0 41.0 35.0 41.0 26-27 38.68523889096546 40.0 38.0 41.0 35.0 41.0 28-29 38.529017611749964 40.0 38.0 41.0 34.0 41.0 30-31 38.38464743519272 40.0 38.0 41.0 34.0 41.0 32-33 38.32951507222796 40.0 38.0 41.0 34.0 41.0 34-35 38.20353554231437 40.0 38.0 41.0 34.0 41.0 36-37 38.019594446032414 40.0 37.5 41.0 33.5 41.0 38-39 37.85409126888151 40.0 37.0 41.0 33.0 41.0 40-41 37.68741177634617 40.0 37.0 41.0 33.0 41.0 42-43 37.43198973197599 40.0 37.0 41.0 33.0 41.0 44-45 36.9978864800686 40.0 36.5 41.0 31.5 41.0 46-47 36.63983421648601 40.0 36.0 41.0 31.0 41.0 48-49 36.21683010487896 39.5 36.0 41.0 30.0 41.0 50-51 35.91924100173699 39.0 35.5 41.0 29.0 41.0 52-53 35.69771113212111 39.0 35.0 40.5 28.5 41.0 54-55 35.46874189221873 39.0 35.0 40.0 28.0 41.0 56-57 35.1843857874717 38.5 35.0 40.0 27.0 41.0 58-59 34.861908269387214 38.0 34.5 40.0 26.0 41.0 60-61 34.49474230997559 38.0 34.0 40.0 26.0 41.0 62-63 34.17122479716805 37.5 34.0 40.0 25.5 41.0 64-65 33.789362041291966 37.0 33.5 39.5 24.5 41.0 66-67 33.396098920428315 36.0 33.0 39.0 23.0 40.5 68-69 32.943138343484094 36.0 32.5 39.0 22.0 40.0 70-71 32.4447978276643 35.0 32.0 38.0 21.0 40.0 72-73 32.100486466876276 35.0 32.0 37.5 20.0 40.0 74-75 31.671098920428314 35.0 31.0 37.0 18.5 39.0 76-77 30.312334271454013 33.5 29.5 35.5 18.0 38.5 78-79 31.002673643939225 34.0 31.0 36.0 18.0 38.5 80-81 30.79559816186979 34.0 31.0 36.0 15.0 37.5 82-83 30.446548009058727 34.0 31.0 35.0 10.0 37.0 84-85 30.06868692421011 34.0 30.5 35.0 7.0 37.0 86-87 29.750435896308346 34.0 30.0 35.0 4.0 36.0 88-89 29.39370121589235 34.0 30.0 35.0 2.0 36.0 90-91 28.977043710560455 34.0 29.0 35.0 2.0 35.5 92-93 28.547422550075858 34.0 29.0 35.0 2.0 35.0 94-95 28.07506321320991 34.0 29.0 35.0 2.0 35.0 96-97 27.450452386710936 33.0 27.0 35.0 2.0 35.0 98-99 26.793130098282802 33.0 26.5 35.0 2.0 35.0 100-101 23.844054110507685 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22227326138479953 1101 2 0.05937812250096641 1101 3 0.04087259637591245 1101 4 0.12146985257266607 1101 5 0.13278617492564138 1101 6 0.13688998189500978 1101 7 0.14599100581718005 1101 8 0.16446332472591507 1101 9 0.22689466936112268 1101 10-11 0.216275344836113 1101 12-13 0.1823153228222978 1101 14-15 0.29413745947893233 1101 16-17 0.2900652674678952 1101 18-19 0.3095129168709079 1101 20-21 0.2271999179714541 1101 22-23 0.1590599491836855 1101 24-25 0.2927969849913623 1101 26-27 0.20100107918338495 1101 28-29 0.2465071717342937 1101 30-31 0.27876134993913837 1101 32-33 0.3803705580374128 1101 34-35 0.40786267896432804 1101 36-37 0.443141421945775 1101 38-39 0.43553975635434483 1101 40-41 0.3654201646988824 1101 42-43 0.4529573232945481 1101 44-45 0.412220066665391 1101 46-47 0.6105233710667761 1101 48-49 0.4147086667659394 1101 50-51 0.40370980450516214 1101 52-53 0.36203409745426995 1101 54-55 0.3349598921112431 1101 56-57 0.2986517714942778 1101 58-59 0.3319136175633375 1101 60-61 0.38613129958768155 1101 62-63 0.44343666740083876 1101 64-65 0.3342761813969872 1101 66-67 0.4053315316272119 1101 68-69 0.5168101281893911 1101 70-71 0.49860461738178685 1101 72-73 0.59617455586978 1101 74-75 0.5672449897868042 1101 76-77 0.39813175445208415 1101 78-79 0.7039457963511637 1101 80-81 0.5252700706570046 1101 82-83 0.6828995976388548 1101 84-85 0.6906231894356907 1101 86-87 0.4743901087069098 1101 88-89 0.598434250505111 1101 90-91 0.9178854517474342 1101 92-93 0.982235442902482 1101 94-95 1.1013951407440707 1101 96-97 0.9134175665113062 1101 98-99 0.9421181610390619 1101 100-101 0.9653000905814721 1102 1 0.2592821708680262 1102 2 0.05790025202655613 1102 3 0.13862583551002672 1102 4 0.1615140519621292 1102 5 0.14803658012814225 1102 6 0.1590176430562238 1102 7 0.1821018821483804 1102 8 0.23455962868248292 1102 9 0.3270033034060873 1102 10-11 0.23161586409193546 1102 12-13 0.28262749033252277 1102 14-15 0.4215211315582508 1102 16-17 0.3139200110716658 1102 18-19 0.329863777491191 1102 20-21 0.22752774668266795 1102 22-23 0.3023012087419943 1102 24-25 0.259849111931608 1102 26-27 0.30140273927068506 1102 28-29 0.19941418894669738 1102 30-31 0.2770606802363531 1102 32-33 0.35681163711901576 1102 34-35 0.31507596712047814 1102 36-37 0.49700725268548496 1102 38-39 0.5015181588124094 1102 40-41 0.4811921749010466 1102 42-43 0.49392006455162374 1102 44-45 0.8297218938055053 1102 46-47 0.7711532247961301 1102 48-49 0.7810092425015185 1102 50-51 0.868707641767891 1102 52-53 0.9459368065934513 1102 54-55 0.8364856162553451 1102 56-57 0.92412968671497 1102 58-59 0.875012994098384 1102 60-61 1.0378954841611545 1102 62-63 0.9232069946453549 1102 64-65 0.8069290521199903 1102 66-67 0.7806733435535875 1102 68-69 0.7609741556536704 1102 70-71 0.6569188506789985 1102 72-73 0.7673797598631396 1102 74-75 0.6044151723516613 1102 76-77 0.6570403054678238 1102 78-79 0.5787226863846762 1102 80-81 0.5721116663096559 1102 82-83 0.768716142827639 1102 84-85 0.7790120247395187 1102 86-87 0.8196195328718971 1102 88-89 0.944261224065027 1102 90-91 0.9569542441942609 1102 92-93 0.9830601418593936 1102 94-95 1.135554270975657 1102 96-97 1.3737819877429231 1102 98-99 1.4175878727735274 1102 100-101 1.1526792134547819 1103 1 -0.029013038542203162 1103 2 -0.21627146843418643 1103 3 -0.094421907580859 1103 4 -0.0703075524987824 1103 5 0.022927194316750388 1103 6 0.07866486109521986 1103 7 0.025026400850812536 1103 8 0.12055926236720893 1103 9 0.13021568486239232 1103 10-11 0.10425516565080528 1103 12-13 0.05499688876142983 1103 14-15 0.14974445159569427 1103 16-17 0.2098508588880179 1103 18-19 0.2256419939428227 1103 20-21 0.17396812887159996 1103 22-23 0.22047934989547713 1103 24-25 0.11463181574536208 1103 26-27 0.19254477801869996 1103 28-29 0.19624454427251692 1103 30-31 0.32405969322019246 1103 32-33 0.38804510205450526 1103 34-35 0.3695284784152051 1103 36-37 0.5148193329492372 1103 38-39 0.5213297099540881 1103 40-41 0.4784282752714262 1103 42-43 0.5317828184284608 1103 44-45 0.5906719222966998 1103 46-47 0.6612153966386956 1103 48-49 0.7710068003996824 1103 50-51 0.7762054031745365 1103 52-53 0.8774447535998391 1103 54-55 0.8127132811989668 1103 56-57 0.7899896118238487 1103 58-59 0.7505319407384619 1103 60-61 0.8127629492594508 1103 62-63 0.7002659917555363 1103 64-65 0.578252925700518 1103 66-67 0.7954734982200407 1103 68-69 0.6966133249320237 1103 70-71 0.48383742693362564 1103 72-73 0.5403240697658589 1103 74-75 0.5173173559977151 1103 76-77 0.5345243809287687 1103 78-79 0.7457751712232898 1103 80-81 0.7714316935797925 1103 82-83 0.69144651443413 1103 84-85 0.6139869931957058 1103 86-87 0.43984471501198996 1103 88-89 0.6296180253186385 1103 90-91 0.8764068572432286 1103 92-93 0.9200462829314766 1103 94-95 0.9175470769506688 1103 96-97 0.9985924704650522 1103 98-99 1.233455938221855 1103 100-101 1.3700936890134088 1104 1 0.15636047525425312 1104 2 0.07231149724292152 1104 3 0.10535824646086667 1104 4 0.07360854710489662 1104 5 0.08263107527091051 1104 6 0.07579789033323436 1104 7 0.12847588862468484 1104 8 0.19910334372517013 1104 9 0.14579782798659835 1104 10-11 0.05217223736246268 1104 12-13 0.06601834991450062 1104 14-15 0.1427748112756433 1104 16-17 0.19830034666189533 1104 18-19 0.238737783967494 1104 20-21 0.14870341639123552 1104 22-23 0.21231850341376202 1104 24-25 0.0028300509954917175 1104 26-27 -0.009259977832641653 1104 28-29 0.24133352954134324 1104 30-31 0.2515127703158342 1104 32-33 0.3067645367363241 1104 34-35 0.24719953203069167 1104 36-37 0.43839818586182133 1104 38-39 0.43389084059045047 1104 40-41 0.5313625458165561 1104 42-43 0.6623451455054052 1104 44-45 0.5479324472350058 1104 46-47 0.5898675315373723 1104 48-49 0.6682774203054649 1104 50-51 0.7029282776230517 1104 52-53 0.6487006423308657 1104 54-55 0.7781481125756216 1104 56-57 0.855200484477713 1104 58-59 0.8421531177368919 1104 60-61 0.8375345038844344 1104 62-63 0.8745559790433575 1104 64-65 0.8905841154111229 1104 66-67 0.8820804674864675 1104 68-69 0.5430550358420732 1104 70-71 0.6882810820596248 1104 72-73 0.690941358332374 1104 74-75 0.7255040007296394 1104 76-77 0.6625308117969375 1104 78-79 0.6763139433761367 1104 80-81 0.7285920166187516 1104 82-83 0.7702358661128876 1104 84-85 0.8114759635090216 1104 86-87 0.9805666921300613 1104 88-89 0.9521612093461549 1104 90-91 1.101071479990555 1104 92-93 1.3021733299665499 1104 94-95 1.0440901114231131 1104 96-97 1.6058025205660051 1104 98-99 1.4238500158409408 1104 100-101 1.6026383685916663 1105 1 0.11683943427972565 1105 2 -0.08623778293426909 1105 3 -0.003502956935221846 1105 4 -0.004499610886981031 1105 5 0.05543561569173505 1105 6 0.038111474681699065 1105 7 0.12104602520645358 1105 8 0.039072889683083645 1105 9 0.09378047841111226 1105 10-11 0.15206241824102307 1105 12-13 0.19025884456197417 1105 14-15 0.2267250696735772 1105 16-17 0.17293397530714572 1105 18-19 0.027736491977833566 1105 20-21 0.11865819675298894 1105 22-23 0.10362479724912532 1105 24-25 0.08383318868467171 1105 26-27 0.1621496751758542 1105 28-29 0.1347605321253198 1105 30-31 0.3608649297842774 1105 32-33 0.2421655149570725 1105 34-35 0.18428055823963518 1105 36-37 0.17957205075661165 1105 38-39 0.15088650319657404 1105 40-41 -0.06921469670836444 1105 42-43 -0.0029090996034923933 1105 44-45 0.08493964546313038 1105 46-47 0.12486153301392733 1105 48-49 0.1464151094896664 1105 50-51 0.03435085052818465 1105 52-53 0.24403932819280527 1105 54-55 0.24468834882516433 1105 56-57 0.3530590116094956 1105 58-59 0.13202022737144148 1105 60-61 0.3504645518726264 1105 62-63 0.062359596614413704 1105 64-65 0.10260792493493653 1105 66-67 0.2010759455226463 1105 68-69 -0.06336153853962401 1105 70-71 -0.07205944950184318 1105 72-73 0.07417225534234007 1105 74-75 -0.08068001551251669 1105 76-77 0.010070494336620328 1105 78-79 -0.051922029307508666 1105 80-81 0.14553692248807693 1105 82-83 0.22915290191945914 1105 84-85 0.49019597089181843 1105 86-87 0.24478780693257107 1105 88-89 0.5473102495824058 1105 90-91 0.5236485379455189 1105 92-93 0.5226610560647416 1105 94-95 0.5960977710761206 1105 96-97 0.6040223433788476 1105 98-99 0.605018805062052 1105 100-101 0.3364432781523874 1106 1 0.2419688338656414 1106 2 0.2291678259496024 1106 3 0.14025606182379846 1106 4 0.18918412084023117 1106 5 0.0932791127743613 1106 6 0.22725053610336943 1106 7 0.1908002444171899 1106 8 0.1586498706731092 1106 9 0.26995997524277726 1106 10-11 0.34307581305876766 1106 12-13 0.2839715931660223 1106 14-15 0.2505365540828848 1106 16-17 0.3267116589761372 1106 18-19 0.26865107256632115 1106 20-21 0.2896342617507983 1106 22-23 0.37429540080016466 1106 24-25 0.5863702048086452 1106 26-27 0.4732243191309351 1106 28-29 0.4182064038864084 1106 30-31 0.4594203598614399 1106 32-33 0.4038463763661895 1106 34-35 0.2918978805308683 1106 36-37 0.49568347556368764 1106 38-39 0.5322172799077833 1106 40-41 0.4539301837734726 1106 42-43 0.4788435981818182 1106 44-45 0.7575234022860187 1106 46-47 0.6109001019329341 1106 48-49 0.338075502237011 1106 50-51 0.3496525476233572 1106 52-53 0.5089751773677946 1106 54-55 0.5018837500423032 1106 56-57 0.5580573647056681 1106 58-59 0.36404544852710075 1106 60-61 0.3725291418719934 1106 62-63 0.4765010107558325 1106 64-65 0.44995170434393117 1106 66-67 0.5086215098616904 1106 68-69 0.35203073826569664 1106 70-71 0.4381631177859404 1106 72-73 0.5041435521614659 1106 74-75 0.5405752388949132 1106 76-77 0.37776137833185075 1106 78-79 0.39843362349379774 1106 80-81 0.4714415432296555 1106 82-83 0.6796301119075387 1106 84-85 0.7742672739848615 1106 86-87 0.5656909376183137 1106 88-89 0.8001472374737411 1106 90-91 0.8239516466725405 1106 92-93 0.8925419300217037 1106 94-95 0.6537374442049249 1106 96-97 1.0329349163131631 1106 98-99 0.9887101439164354 1106 100-101 0.580651666382515 1107 1 0.16238671679697347 1107 2 0.06709386774721082 1107 3 0.12430146283032428 1107 4 0.11350336179797438 1107 5 0.1507473269486752 1107 6 0.10142475641371362 1107 7 0.10543592368645704 1107 8 0.07915155365751048 1107 9 0.16954888942687063 1107 10-11 0.13775637191200474 1107 12-13 0.1878645711871343 1107 14-15 0.21554770414550717 1107 16-17 0.25389406841388507 1107 18-19 0.21381324894681342 1107 20-21 0.2681232536827096 1107 22-23 0.2824924426582882 1107 24-25 0.18417883342078056 1107 26-27 0.18573659816648558 1107 28-29 0.243650893334582 1107 30-31 0.1499234860689782 1107 32-33 0.1625883513472246 1107 34-35 0.11847134291627981 1107 36-37 0.05682259761847064 1107 38-39 0.11741400053237783 1107 40-41 0.3275178037123041 1107 42-43 0.16777314561497292 1107 44-45 0.24715780127669973 1107 46-47 0.20996340356191467 1107 48-49 0.11941555819682748 1107 50-51 0.0019405218501873378 1107 52-53 -0.16564783878234124 1107 54-55 -0.11155358846801278 1107 56-57 -0.21389475753067444 1107 58-59 -0.08138209468813073 1107 60-61 -0.05453222304464589 1107 62-63 -0.1710522604723579 1107 64-65 -0.21817913547226198 1107 66-67 -0.28244633559238963 1107 68-69 0.02587860392490171 1107 70-71 -0.09028467245320826 1107 72-73 -0.1263051971227398 1107 74-75 -0.2814869873000525 1107 76-77 -0.26095564285559547 1107 78-79 -0.06291360202611074 1107 80-81 0.12428818447697054 1107 82-83 0.2020955094689576 1107 84-85 0.16441704330193474 1107 86-87 0.09405725558364608 1107 88-89 -0.08843593640671443 1107 90-91 0.24400406826129384 1107 92-93 0.38270473490258894 1107 94-95 0.5724863094125396 1107 96-97 0.4937560637221097 1107 98-99 0.48325615712891334 1107 100-101 0.48954005867850015 1108 1 -0.04186004207438998 1108 2 -0.28174588308154114 1108 3 -0.07863386840634234 1108 4 0.026694138090277875 1108 5 -0.01935778964291046 1108 6 0.052092410698719505 1108 7 0.03318192767740413 1108 8 0.012839458166148177 1108 9 0.024008755288868144 1108 10-11 0.03658418227309568 1108 12-13 0.016709098202944972 1108 14-15 0.03611080850519244 1108 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-0.9277181419480378 2316 86-87 -0.8406475223146401 2316 88-89 -0.9787436950977764 2316 90-91 -1.07020950857342 2316 92-93 -0.98562414745561 2316 94-95 -0.9633916600098438 2316 96-97 -1.0084344380135768 2316 98-99 -0.8828161391466516 2316 100-101 -0.8598576019356194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 6.0 5 22.0 6 35.0 7 70.0 8 187.0 9 414.0 10 690.0 11 1184.0 12 2135.0 13 2962.0 14 3866.0 15 5586.0 16 7925.0 17 11800.0 18 10801.0 19 5813.0 20 4492.0 21 4375.0 22 4364.0 23 4664.0 24 5335.0 25 6177.0 26 7541.0 27 9229.0 28 11502.0 29 14420.0 30 18359.0 31 23447.0 32 31091.0 33 40810.0 34 58101.0 35 86237.0 36 136769.0 37 209297.0 38 164654.0 39 15253.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49627029784838 19.625028588518852 15.225915272426594 20.65278584120617 2 15.559840720845914 21.882304050800464 39.641577927831854 22.91627730052177 3 20.811794324915624 25.699600919095417 30.200969667652462 23.287635088336504 4 12.923872522451706 17.67817075061097 38.19203424730574 31.20592247963158 5 13.139960444455683 38.26454721654517 33.90519202248883 14.690300316510315 6 30.141960535125143 37.638175983571145 17.25656566100752 14.963297820296189 7 27.198640309844013 34.323711356073254 20.850487951548946 17.627160382533784 8 26.054726149381057 33.83720674567402 19.694707680130165 20.413359424814757 9 26.363646357819746 17.70299685582991 20.915107407488843 35.018249378861505 10-11 24.742983192422766 27.15525557664496 26.92735808763994 21.174403143292334 12-13 26.984916612614196 24.833225228394586 27.423181363441472 20.75867679554974 14-15 23.086343747938702 26.03812581077813 27.660946329236385 23.214584112046786 16-17 21.074954376552846 29.180591895516812 27.9056639036081 21.838789824322244 18-19 20.784668323035994 27.890822541280976 30.940887403531146 20.383621732151887 20-21 22.31224421925203 25.88318395325379 30.975684166261264 20.828887661232915 22-23 21.579450759657878 26.13860733053363 31.09853565225039 21.1834062575581 24-25 22.127564137448815 26.158118579864876 31.230461653306246 20.483855629380066 26-27 21.65921288997654 25.9177599971856 31.924041837894652 20.49898527494322 28-29 20.83924025653133 27.29550960233466 31.324933638157137 20.540316502976868 30-31 21.42170334578908 26.185765264245266 31.170776036807585 21.221755353158073 32-33 21.4273896053425 26.472730390941628 31.2175582652594 20.882321738456472 34-35 22.066555592262755 26.22524943642841 31.0803479109785 20.627847060330332 36-37 21.927221674963498 25.88150187338388 31.546179352319303 20.64509709933332 38-39 21.352336641761838 26.725216469729418 31.389155185379003 20.53329170312974 40-41 21.473285992750494 26.83087450347908 30.89346829687141 20.802371206899014 42-43 21.207347205769587 27.104351100349326 30.16598000764074 21.522321686240343 44-45 21.888099664362755 26.413430766710675 30.17327149018319 21.525198078743376 46-47 22.51172056008034 27.23726789058502 29.584018372517303 20.66699317681734 48-49 22.41651023644554 27.31510772385096 29.289893828999553 20.97848821070395 50-51 21.48290739469349 27.657858761667665 29.847787836329186 21.011446007309658 52-53 22.1994713115903 28.250529485455022 28.43511418911082 21.114885013843853 54-55 21.575036362976718 27.61222499639943 28.76314206902314 22.04959657160071 56-57 22.26652059746189 27.634289031232463 28.630095496498793 21.469094874806853 58-59 22.997484625211357 27.348434377081944 28.32616539468737 21.327915603019328 60-61 22.051813414540298 27.61755037873374 29.613460713932344 20.717175492793615 62-63 21.707801059783204 27.65127195971944 29.4408654163277 21.200061564169655 64-65 21.485153358585734 28.440007409716074 28.85567709029025 21.219162141407946 66-67 21.873993397235548 27.68279906202487 28.3612149702238 22.081992570515787 68-69 21.72507295632954 27.73615781104077 28.628069209158337 21.910700023471353 70-71 22.507250296278215 27.694097962001628 28.337225076900413 21.46142666481974 72-73 22.262593170774608 27.1927837998285 29.08230909610607 21.462313933290826 74-75 21.6846495791894 28.783488811456735 28.181309973961465 21.350551635392396 76-77 22.019791922613287 27.89937704310677 28.00249776416027 22.07833327011967 78-79 22.127176323273808 28.029757513860197 28.021072558950504 21.821993603915484 80-81 22.546355670066255 28.053570338967678 27.856939071792674 21.54313491917339 82-83 22.334266701406868 27.891544608727486 28.39890546377619 21.37528322608945 84-85 21.94862385119376 28.2974497724513 28.145512433960302 21.60841394239464 86-87 21.865298018487085 28.37749887863783 27.843862411060588 21.913340691814494 88-89 21.771315681851164 28.31190661512821 27.42559464914711 22.49118305387352 90-91 22.069020106220297 27.717264041318394 27.741559927837915 22.472155924623387 92-93 22.49554759130186 28.105307710912246 27.78160110815505 21.617543589630834 94-95 22.138401978411828 27.881798798067543 27.91087692257798 22.06892230094265 96-97 21.28770180462948 28.08779635116342 28.652217171189694 21.97228467301741 98-99 21.7324074272575 28.550163231898136 27.33588721580087 22.381542125043495 100-101 21.28155811574502 28.568790082359335 27.258780259484404 22.890871542411237 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 422.0 1 323.0 2 244.5 3 285.5 4 258.5 5 232.5 6 400.5 7 905.5 8 1530.5 9 1859.0 10 1760.0 11 1562.0 12 1546.0 13 1672.0 14 1821.5 15 1913.0 16 2000.0 17 2136.0 18 2288.0 19 2404.0 20 2538.0 21 2764.0 22 3165.0 23 3678.0 24 4259.0 25 5057.5 26 6173.0 27 7523.0 28 8711.5 29 10168.0 30 12020.5 31 13617.5 32 15447.5 33 17328.5 34 19198.0 35 20933.0 36 23122.0 37 25066.0 38 25895.0 39 26430.5 40 27004.0 41 28153.5 42 28689.5 43 29281.5 44 30484.5 45 31011.5 46 31596.5 47 37025.5 48 47640.5 49 45643.5 50 34711.0 51 29992.0 52 27726.5 53 26620.5 54 25324.5 55 23377.0 56 21135.0 57 18735.5 58 17127.0 59 15453.5 60 12924.5 61 10467.0 62 8330.0 63 6360.0 64 4583.0 65 3261.0 66 2384.5 67 1788.5 68 1313.0 69 886.5 70 608.5 71 427.0 72 307.5 73 240.5 74 175.5 75 137.5 76 93.5 77 51.5 78 37.5 79 20.5 80 10.0 81 7.5 82 7.5 83 7.0 84 4.0 85 1.0 86 0.0 87 0.5 88 0.5 89 1.0 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01802950682702667 2 0.0024185923792352853 3 0.0032980805171390253 4 1.0993601723796751E-4 5 0.0014291682240935777 6 1.0993601723796751E-4 7 1.0993601723796751E-4 8 0.0 9 0.0 10-11 5.4968008618983754E-5 12-13 0.001099360172379675 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0030232404740441064 22-23 0.0 24-25 0.003353048525758009 26-27 0.001539104241331545 28-29 0.0025834964050922365 30-31 0.014676458301268663 32-33 9.344561465227237E-4 34-35 0.002143752336140366 36-37 0.00395769662056683 38-39 0.00263846441371122 40-41 0.0045073767067566675 42-43 0.0024735603878542687 44-45 7.695521206657725E-4 46-47 5.4968008618983754E-5 48-49 2.7484004309491874E-4 50-51 1.6490402585695124E-4 52-53 0.0011543281809986588 54-55 0.0045073767067566675 56-57 6.046480948088213E-4 58-59 0.001539104241331545 60-61 0.001099360172379675 62-63 0.0 64-65 3.8477606033288623E-4 66-67 1.0993601723796751E-4 68-69 5.4968008618983754E-5 70-71 2.1987203447593502E-4 72-73 0.0 74-75 7.145841120467888E-4 76-77 7.145841120467888E-4 78-79 1.0993601723796751E-4 80-81 0.005441832853279391 82-83 0.0012092961896176427 84-85 0.0037927925947098787 86-87 0.00131923220685561 88-89 8.794881379037401E-4 90-91 1.0993601723796751E-4 92-93 0.0 94-95 1.6490402585695124E-4 96-97 0.0016490402585695126 98-99 0.0026934324223302037 100-101 9.344561465227237E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 909620.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.03141185481145 #Duplication Level Percentage of deduplicated Percentage of total 1 84.33738617866385 55.68916681521635 2 9.843260850506336 12.999288224282509 3 2.582874409334839 5.1165253167612486 4 1.0794427425073965 2.851085132167723 5 0.5465370009007227 1.8044304900184538 6 0.35392845471736417 1.4022237336347554 7 0.21617186076295716 0.9991893218621845 8 0.1690617754227126 0.893071017747422 9 0.12570095381456892 0.7470190306685189 >10 0.6888317164776167 8.127009751915898 >50 0.03368255736798867 1.5297162791796803 >100 0.019100369333405202 2.4196699337566576 >500 0.0015079238213643405 0.6483135399110587 >1k 0.0020105650951524542 2.607304640799304 >5k 5.026412737881136E-4 2.1659867720782775 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7851 0.8631076713352828 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6457 0.7098568633055562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5270 0.5793628108440888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3378 0.37136386622985423 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2754 0.30276379147336246 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2356 0.2590092566126514 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1998 0.21965216244145908 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1901 0.2089883687693762 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1859 0.20437105604538158 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1778 0.1954662386491062 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1733 0.1905191178733977 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1608 0.17677711571865176 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1504 0.16534376992590313 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1493 0.16413447373628548 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1205 0.13247290077175083 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 944 0.10377960027264131 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0993601723796751E-4 0.0 10-11 0.0 0.0 0.0 1.0993601723796751E-4 0.0 12-13 0.0 0.0 0.0 1.0993601723796751E-4 0.0 14-15 0.0 0.0 0.0 1.0993601723796751E-4 0.0 16-17 0.0 0.0 0.0 1.0993601723796751E-4 0.0 18-19 0.0 0.0 0.0 1.0993601723796751E-4 0.0 20-21 0.0 0.0 0.0 1.6490402585695127E-4 0.0 22-23 0.0 0.0 0.0 2.1987203447593502E-4 0.0 24-25 0.0 0.0 0.0 3.8477606033288623E-4 0.0 26-27 0.0 0.0 0.0 7.145841120467888E-4 0.0 28-29 0.0 0.0 0.0 0.0020338163189023988 0.0 30-31 0.0 0.0 0.0 0.0045073767067566675 0.0 32-33 0.0 0.0 0.0 0.0075306171808007735 0.0 34-35 0.0 0.0 0.0 0.011103537741034718 0.0 36-37 0.0 0.0 0.0 0.014566522284030693 0.0 38-39 0.0 0.0 0.0 0.017534794749455816 0.0 40-41 0.0 0.0 0.0 0.021492491370022647 0.0 42-43 0.0 0.0 0.0 0.02918801257668037 0.0 44-45 0.0 0.0 0.0 0.03611398166267232 0.0 46-47 0.0 0.0 0.0 0.058595897187836685 0.0 48-49 0.0 0.0 0.0 0.10542864053121084 0.0 50-51 0.0 0.0 0.0 0.13544117323717597 0.0 52-53 0.0 0.0 0.0 0.16188078538290715 0.0 54-55 0.0 0.0 0.0 0.20327169587300192 0.0 56-57 0.0 0.0 0.0 0.23504320485477453 0.0 58-59 0.0 0.0 0.0 0.27357577889668216 0.0 60-61 0.0 0.0 0.0 0.33744860491194123 0.0 62-63 0.0 0.0 0.0 0.3836217321518876 0.0 64-65 0.0 0.0 0.0 0.43765528462434866 0.0 66-67 0.0 0.0 0.0 0.537806996328137 0.0 68-69 0.0 0.0 0.0 0.6477430135661045 0.0 70-71 0.0 0.0 0.0 0.7391548118994745 0.0 72-73 0.0 0.0 0.0 0.8492557331632989 0.0 74-75 0.0 0.0 0.0 0.9388535872122425 0.0 76-77 0.0 0.0 0.0 1.0029462852619775 0.0 78-79 0.0 0.0 0.0 1.0972164200435346 0.0 80-81 0.0 0.0 0.0 1.231448297091093 0.0 82-83 0.0 0.0 0.0 1.3881620896638158 0.0 84-85 0.0 0.0 0.0 1.563235197115279 0.0 86-87 0.0 0.0 0.0 1.8934829928981332 0.0 88-89 0.0 0.0 0.0 2.3183307315142585 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGACG 70 0.008393437 27.14102 8 GAGTACT 5690 0.0 24.332712 12-13 GTACTTT 6000 0.0 22.918457 14-15 AGTACTT 5870 0.0 22.372845 12-13 GTACATG 4660 0.0 21.918571 1 GTATAGG 310 1.0913936E-11 21.454878 1 AGAGTAC 8820 0.0 20.651947 10-11 TACATGG 4800 0.0 20.581942 2 ACTTTTT 6810 0.0 20.193584 16-17 GTGTAGC 425 0.0 20.120708 1 TACTTTT 6580 0.0 20.03204 14-15 ACATGGG 4650 0.0 20.020151 3 GTATAAT 570 0.0 18.336123 1 CTATACC 185 1.5216839E-4 17.971756 4 CTATACA 425 3.6379788E-12 17.881142 4 CATGGGG 1700 0.0 17.322355 4 ATGGGAG 1240 0.0 17.236734 5 CATGGGA 2920 0.0 17.079325 4 CGCGATC 85 0.0017882563 16.764492 22-23 GTATCAA 16020 0.0 16.695736 1 >>END_MODULE