FastQCFastQC Report
Thu 26 May 2016
SRR936922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725781
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42430.5846116114916208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41980.5784113940706632No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34870.4804479588195337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.43318852381090167No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23430.32282465371785707No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20810.2867256100669486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.271293957819232No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14670.2021270879232165No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14500.19978478356418808No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC11320.15596991378942132No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11160.1537653920397475No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9920.1366803484797756No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8940.12317765276302355No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8740.12042200057593132No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8250.11367065271755529No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA7380.101683565703704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGA455.694965E-736.94806376-77
ACGCGAA605.3788553E-627.71104678-79
TAACGCG605.3788553E-627.71104676-77
GCACCGT1056.884847E-527.1530026
TATAGGG1955.329639E-1026.803042
GTGTAGG3150.025.6427351
GTATAGG2600.025.5847191
ATAACGC651.0015823E-525.57942874-75
TACATGG33600.024.1814732
GTACATG34550.023.9291421
TGCACCG1801.9406616E-723.7605155
GAGTACT31350.023.49119612-13
GTACTAG1452.3273196E-522.9380231
ACATGGG34150.022.818083
GTACTTT34550.021.0433214-15
GCGTATT804.982712E-520.78543594-95
CTTTGCG1404.8394082E-420.3633482
TTTGCGT1404.8394082E-420.3633483
AGTACTT32700.020.1965912-13
GTATACA3553.6379788E-1220.076541
Produced by FastQC (version 0.11.5)