##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936922_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 725781 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.999231173039803 33.0 31.0 34.0 30.0 34.0 2 32.11646488403527 33.0 31.0 34.0 30.0 34.0 3 32.13265158498225 34.0 31.0 34.0 30.0 34.0 4 35.69557896941364 37.0 35.0 37.0 35.0 37.0 5 35.70230551640233 37.0 35.0 37.0 35.0 37.0 6 35.780552260254815 37.0 35.0 37.0 35.0 37.0 7 35.73893502309925 37.0 35.0 37.0 35.0 37.0 8 35.69136557721957 37.0 35.0 37.0 33.0 37.0 9 37.36889089132948 39.0 37.0 39.0 35.0 39.0 10-11 37.202908315318254 39.0 37.0 39.0 34.0 39.0 12-13 37.16371329643515 39.0 37.0 39.0 34.0 39.0 14-15 38.34113940706632 40.0 38.0 41.0 34.0 41.0 16-17 38.28911613833925 40.0 38.0 41.0 34.0 41.0 18-19 38.28663880702305 40.0 38.0 41.0 33.5 41.0 20-21 38.22639542782189 40.0 38.0 41.0 34.0 41.0 22-23 38.123780451678954 40.0 38.0 41.0 33.5 41.0 24-25 37.96453475635212 40.0 38.0 41.0 33.0 41.0 26-27 38.03457792364363 40.0 38.0 41.0 33.0 41.0 28-29 37.995292657151396 40.0 38.0 41.0 33.0 41.0 30-31 37.84712399470364 40.0 37.5 41.0 33.0 41.0 32-33 37.621824627539155 40.0 37.0 41.0 33.0 41.0 34-35 37.430140083578934 40.0 37.0 41.0 32.5 41.0 36-37 37.24056430245487 40.0 36.5 41.0 31.5 41.0 38-39 37.07638461188706 40.0 36.0 41.0 31.0 41.0 40-41 36.9196851391811 39.0 36.0 41.0 31.0 41.0 42-43 36.69449324245193 39.0 36.0 41.0 30.5 41.0 44-45 36.39177520491718 39.0 35.5 41.0 30.0 41.0 46-47 35.99278225800896 39.0 35.0 40.5 29.5 41.0 48-49 35.6067339872496 39.0 35.0 40.0 27.5 41.0 50-51 34.95902069632575 38.5 34.0 39.5 26.5 40.5 52-53 35.20326723901563 38.5 35.0 40.0 27.5 40.5 54-55 35.597341346769895 39.0 35.0 40.5 28.0 41.0 56-57 35.46492674787574 39.0 35.0 41.0 27.0 41.0 58-59 35.2159115490761 39.0 35.0 41.0 26.5 41.0 60-61 34.9142103471984 38.5 35.0 40.0 26.0 41.0 62-63 34.63955793827614 38.0 34.0 40.0 26.0 41.0 64-65 34.332972342896824 37.5 34.0 40.0 25.5 41.0 66-67 33.9339793959886 37.0 34.0 40.0 24.0 41.0 68-69 33.53224664189335 36.5 33.5 39.0 22.5 41.0 70-71 33.074949606010634 36.0 33.0 39.0 22.0 41.0 72-73 32.677747833024014 35.5 33.0 39.0 21.0 40.0 74-75 32.26111320081402 35.0 32.0 37.5 20.0 40.0 76-77 31.831414021585026 35.0 32.0 37.0 18.0 39.0 78-79 31.362435776081213 35.0 31.5 36.5 15.5 39.0 80-81 30.88780362120254 35.0 31.0 36.0 10.0 38.5 82-83 30.44529823734708 35.0 31.0 36.0 7.0 37.0 84-85 30.09057415391144 34.0 30.5 35.0 4.5 37.0 86-87 29.7897781837772 34.0 30.0 35.0 2.0 36.5 88-89 29.415537193726482 34.0 30.0 35.0 2.0 36.0 90-91 29.028800698833393 34.0 29.0 35.0 2.0 36.0 92-93 28.633200648680525 34.0 29.0 35.0 2.0 35.5 94-95 28.224825394988294 34.0 29.0 35.0 2.0 35.0 96-97 27.767377487148327 34.0 28.5 35.0 2.0 35.0 98-99 27.175492331708874 34.0 27.0 35.0 2.0 35.0 100-101 25.033000312766525 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22388056435476145 1101 2 0.1859160393902215 1101 3 0.12600903705530442 1101 4 0.1054229498772088 1101 5 0.11638774510080196 1101 6 0.07113039012604361 1101 7 0.11892509070865742 1101 8 0.08109533600392638 1101 9 0.08233899659811783 1101 10-11 0.13964696716134029 1101 12-13 0.10550740605551567 1101 14-15 0.20948948159050218 1101 16-17 0.31549635640011076 1101 18-19 0.18712118551336943 1101 20-21 0.20438031635998755 1101 22-23 0.4080131222582608 1101 24-25 0.35449317482400744 1101 26-27 0.32652759628371797 1101 28-29 0.3980438005169873 1101 30-31 0.24761358268152378 1101 32-33 0.5099152023367068 1101 34-35 0.3778871801920971 1101 36-37 0.3716044255047706 1101 38-39 0.3666833578167683 1101 40-41 0.35267750090061156 1101 42-43 0.3056407138355439 1101 44-45 0.4872012245989623 1101 46-47 0.43541265925679085 1101 48-49 0.32069895006223703 1101 50-51 0.37733223358072365 1101 52-53 0.49473958087399694 1101 54-55 0.3532577836743158 1101 56-57 0.3938759620106609 1101 58-59 0.23094065495366323 1101 60-61 0.32794828299913803 1101 62-63 0.31858765482416374 1101 64-65 0.42779952558603185 1101 66-67 0.25940414879727314 1101 68-69 0.24396044000061323 1101 70-71 0.2221340489576349 1101 72-73 0.4763947725752331 1101 74-75 0.3264617081518182 1101 76-77 0.30080568640256544 1101 78-79 0.20451689221264857 1101 80-81 0.1867198914986794 1101 82-83 0.3314917730574827 1101 84-85 0.4433972933728825 1101 86-87 0.24372766004635693 1101 88-89 0.2541773149525852 1101 90-91 0.3352743142832253 1101 92-93 0.3162168166040935 1101 94-95 0.16545136779616243 1101 96-97 0.2582084064551289 1101 98-99 0.23180384875209725 1101 100-101 0.18476391627801192 1102 1 0.14206238253657588 1102 2 0.04789951046459606 1102 3 0.16237267341893613 1102 4 0.08393534657142965 1102 5 0.00316460460494028 1102 6 -0.08589440326238673 1102 7 0.13380112376651 1102 8 0.16704574922707138 1102 9 0.09225635197002191 1102 10-11 0.29006019030183694 1102 12-13 0.37038343911336824 1102 14-15 0.3983324567971138 1102 16-17 0.40392610846623 1102 18-19 0.3115013508026223 1102 20-21 0.2589257709054351 1102 22-23 0.2650379156466869 1102 24-25 0.29540226573309525 1102 26-27 0.23727139793660967 1102 28-29 0.24473801539301832 1102 30-31 0.16331606202037108 1102 32-33 0.20536974779125217 1102 34-35 0.1741681719276329 1102 36-37 0.2819350040171713 1102 38-39 0.3443693082299859 1102 40-41 0.2675535339584627 1102 42-43 0.3729960857363679 1102 44-45 0.39092023286342936 1102 46-47 0.3457432377691916 1102 48-49 0.511608040971332 1102 50-51 0.6393157046551039 1102 52-53 0.495152804014495 1102 54-55 0.5458197671453888 1102 56-57 0.6199090198619004 1102 58-59 0.6111059442098608 1102 60-61 0.39778299374293624 1102 62-63 0.4685876548241694 1102 64-65 0.4397829966604121 1102 66-67 0.2151892727642135 1102 68-69 0.4852827540502034 1102 70-71 0.5717208258171382 1102 72-73 0.6693699791868042 1102 74-75 0.5760484850113272 1102 76-77 0.5706403971463736 1102 78-79 0.6884011897333089 1102 80-81 0.6623397262094244 1102 82-83 0.4802521036359977 1102 84-85 0.6909179545299047 1102 86-87 0.4986863377323054 1102 88-89 0.531036819084818 1102 90-91 0.5208115043658701 1102 92-93 0.8182829323065697 1102 94-95 0.670823268622609 1102 96-97 0.5544893981906647 1102 98-99 0.8553575677603611 1102 100-101 0.7959209410714045 1103 1 0.09510640479465238 1103 2 0.27927812822495923 1103 3 0.3016782609860229 1103 4 0.19329625006488982 1103 5 0.1620052749812828 1103 6 0.1819779091408904 1103 7 0.0600267995756667 1103 8 0.3094694428822038 1103 9 0.19283091322911616 1103 10-11 0.29517609033805314 1103 12-13 0.2823734954170831 1103 14-15 0.25978910952503753 1103 16-17 0.4128968371218491 1103 18-19 0.33940296747642407 1103 20-21 0.4019157942830063 1103 22-23 0.5347713784895305 1103 24-25 0.19042794812748554 1103 26-27 0.30226678827607856 1103 28-29 0.2687576393764246 1103 30-31 0.21970620393205564 1103 32-33 0.3473983276865482 1103 34-35 0.3690819054233998 1103 36-37 0.1160119194957474 1103 38-39 0.13518949854311302 1103 40-41 0.35058833689546987 1103 42-43 0.3815568838661605 1103 44-45 0.426318309976466 1103 46-47 0.5633299158502538 1103 48-49 0.4611190876935325 1103 50-51 0.5494075686042663 1103 52-53 0.7595020187258967 1103 54-55 0.7000022109239907 1103 56-57 0.7494849310930078 1103 58-59 0.8099362428500072 1103 60-61 0.8019934247462004 1103 62-63 0.916254014180133 1103 64-65 0.7647887587360742 1103 66-67 0.7378457542761794 1103 68-69 0.6259185579420787 1103 70-71 0.3486363043986813 1103 72-73 0.40880562503317464 1103 74-75 0.3328181007631983 1103 76-77 0.5012082085454033 1103 78-79 0.5208573156428891 1103 80-81 0.6359535354353305 1103 82-83 0.5743039952431204 1103 84-85 0.4720241084267123 1103 86-87 0.5951904041114204 1103 88-89 0.6593137938304601 1103 90-91 0.8277720917683276 1103 92-93 0.6598357293827313 1103 94-95 0.6653888081175232 1103 96-97 0.803299941142825 1103 98-99 0.9058317042721633 1103 100-101 0.5867444239991961 1104 1 0.1701248659113439 1104 2 0.12831704470112015 1104 3 0.1871165301070974 1104 4 0.14741761145617716 1104 5 -0.01657562892805231 1104 6 -0.04714223624868907 1104 7 -0.014483151740044775 1104 8 -0.0360729159842208 1104 9 0.026735030389772874 1104 10-11 0.25726834285959654 1104 12-13 0.1616786941559738 1104 14-15 0.22058963660650477 1104 16-17 0.3756352287274609 1104 18-19 0.2108019442662723 1104 20-21 -0.15773019445903458 1104 22-23 0.05967564599222186 1104 24-25 0.23314774596168064 1104 26-27 -0.10664307725624411 1104 28-29 -0.04308267970242241 1104 30-31 0.2675597398439038 1104 32-33 0.26657401119005186 1104 34-35 0.31211498951324046 1104 36-37 0.29732882722554166 1104 38-39 0.17813757305970057 1104 40-41 0.11888584608180963 1104 42-43 0.016606032443881702 1104 44-45 0.1171239000468205 1104 46-47 0.172808206903575 1104 48-49 0.08454215458247205 1104 50-51 0.11306525749354535 1104 52-53 0.23722818398716328 1104 54-55 0.2877162824956443 1104 56-57 0.22578174163109566 1104 58-59 0.2586513712376828 1104 60-61 0.4166408284720262 1104 62-63 0.3375505151905216 1104 64-65 0.3709247033040839 1104 66-67 0.2008947790428195 1104 68-69 0.262250035583115 1104 70-71 0.26987255805221366 1104 72-73 0.2948861641419924 1104 74-75 0.3320918161126727 1104 76-77 0.24925873673232601 1104 78-79 0.13865526922257843 1104 80-81 0.5189017647157677 1104 82-83 0.5689226350015133 1104 84-85 0.4080054881243491 1104 86-87 0.6019994608920669 1104 88-89 0.5931179777606665 1104 90-91 0.9151318646634259 1104 92-93 0.6680371081075656 1104 94-95 0.9419838707084445 1104 96-97 0.995620006055205 1104 98-99 1.0767938048156083 1104 100-101 0.9945670734679162 1105 1 0.09184147708411317 1105 2 0.19638955672479597 1105 3 0.3177861564482001 1105 4 0.06491668655791472 1105 5 0.21450095264797397 1105 6 0.13791140785045997 1105 7 0.0991683465951354 1105 8 0.08342393936169401 1105 9 0.1819699545481157 1105 10-11 0.2971499558821691 1105 12-13 0.20278117288331288 1105 14-15 0.26113717637712597 1105 16-17 0.16113282399198425 1105 18-19 0.15264434160256002 1105 20-21 0.2658362132361276 1105 22-23 0.3067344695216363 1105 24-25 0.2317788965351113 1105 26-27 0.3250254392069465 1105 28-29 0.3068618264019136 1105 30-31 -0.05097522131036669 1105 32-33 0.2676468931730298 1105 34-35 0.2685337834459034 1105 36-37 0.2884867998485561 1105 38-39 0.3098430854533376 1105 40-41 0.3337212141913213 1105 42-43 0.24675771575872574 1105 44-45 0.3973680731877707 1105 46-47 0.4018870082354269 1105 48-49 0.5778458911714424 1105 50-51 0.577475172402913 1105 52-53 0.3907242994144653 1105 54-55 0.5158701855663068 1105 56-57 0.7942242129592572 1105 58-59 0.5279077530356844 1105 60-61 0.6483833367961154 1105 62-63 0.6992597271771999 1105 64-65 0.7569772375253194 1105 66-67 0.7160261145959055 1105 68-69 0.6414213267410886 1105 70-71 0.6298059653774075 1105 72-73 0.6316601190069591 1105 74-75 0.675118083223289 1105 76-77 0.7090441603357256 1105 78-79 0.5663652211278709 1105 80-81 0.8608921457738496 1105 82-83 0.7359592609726562 1105 84-85 0.70031033459124 1105 86-87 0.7021844405443041 1105 88-89 0.6210129093181962 1105 90-91 0.41598277106372095 1105 92-93 0.6187585287512931 1105 94-95 0.6361385136977162 1105 96-97 0.7484131988040019 1105 98-99 0.6220398277530883 1105 100-101 0.3311930446110871 1106 1 0.15410900031071506 1106 2 0.2251888552898933 1106 3 0.16918143862400115 1106 4 0.14583462204613795 1106 5 0.054993643224129585 1106 6 -0.04271313899898388 1106 7 -0.006911880022293815 1106 8 0.2513720736460101 1106 9 0.1320365571414044 1106 10-11 0.2731822960780974 1106 12-13 0.19334889676076017 1106 14-15 0.28658273929550404 1106 16-17 0.4797874516076064 1106 18-19 0.16094521671005424 1106 20-21 0.18033028644838822 1106 22-23 0.1462357088357109 1106 24-25 0.33022933240272323 1106 26-27 0.40024084379662384 1106 28-29 0.4325662120562157 1106 30-31 0.5689726769141288 1106 32-33 0.2514203094645282 1106 34-35 0.1583604480892049 1106 36-37 0.24076224794067969 1106 38-39 0.3990188994381896 1106 40-41 0.27242912388157237 1106 42-43 0.15024887194601177 1106 44-45 0.48951992710046 1106 46-47 0.5520412901920295 1106 48-49 0.6702236880588259 1106 50-51 0.5753093235149151 1106 52-53 0.4505047637778645 1106 54-55 0.4790482252580759 1106 56-57 0.46282994246841724 1106 58-59 0.5564929116173047 1106 60-61 0.5869007124853169 1106 62-63 0.6480760557724707 1106 64-65 0.6385510820967966 1106 66-67 0.878003621466938 1106 68-69 0.699460849900241 1106 70-71 0.6753722560344997 1106 72-73 0.7471279389944883 1106 74-75 0.6875145962824618 1106 76-77 0.825414066080409 1106 78-79 0.6146790795256969 1106 80-81 0.5686610432058572 1106 82-83 0.7349349299493362 1106 84-85 0.7964515995346666 1106 86-87 0.9128433846824358 1106 88-89 0.9714673244799812 1106 90-91 0.8829268080239494 1106 92-93 0.8026879131409963 1106 94-95 1.0369267848967318 1106 96-97 0.7981636498468809 1106 98-99 1.2346664991409462 1106 100-101 0.5550561636358218 1107 1 0.12830244337375873 1107 2 -0.013957589256939684 1107 3 0.1650946899925998 1107 4 0.2470002432935985 1107 5 0.18783564767184657 1107 6 0.1305718958463018 1107 7 0.08523314604365595 1107 8 0.16274241324702388 1107 9 0.20845313341467886 1107 10-11 0.42482461397018056 1107 12-13 0.4426242380487153 1107 14-15 0.30102875176797994 1107 16-17 0.23096286799160026 1107 18-19 0.4050200660644805 1107 20-21 0.4592830775181227 1107 22-23 0.3574097829232201 1107 24-25 0.3612109864747808 1107 26-27 0.2972631222904951 1107 28-29 0.48919389166093197 1107 30-31 0.3248896649459212 1107 32-33 0.3390096900853976 1107 34-35 0.36025725813973963 1107 36-37 0.4145606204968857 1107 38-39 0.2053971658441398 1107 40-41 0.5571329298362926 1107 42-43 0.34216371230343157 1107 44-45 0.3453600051713295 1107 46-47 0.21391499097600075 1107 48-49 0.05345205622536042 1107 50-51 0.24953668677299845 1107 52-53 0.007439901834871421 1107 54-55 0.07681094351729456 1107 56-57 0.18562939248964483 1107 58-59 0.18779490293227497 1107 60-61 0.17372099349690018 1107 62-63 0.021130179343565203 1107 64-65 0.05288535745621914 1107 66-67 0.16290060928106698 1107 68-69 0.28048567512961853 1107 70-71 0.21871754910861085 1107 72-73 0.09321278664383215 1107 74-75 0.18526352879396768 1107 76-77 0.10687822132244307 1107 78-79 -0.13123054401455292 1107 80-81 0.019207394154712176 1107 82-83 0.19427876463645077 1107 84-85 0.15831750048770132 1107 86-87 0.07531658410053055 1107 88-89 0.038181273842752717 1107 90-91 0.06294237140755143 1107 92-93 0.14817870389873988 1107 94-95 0.029508782886914275 1107 96-97 -0.011704587427605162 1107 98-99 0.04435878674066984 1107 100-101 4.401148487893636E-4 1108 1 0.03186460368816668 1108 2 0.050268903539247844 1108 3 0.2121991073464713 1108 4 0.1209845998049417 1108 5 0.2341754880006519 1108 6 0.009056738814727794 1108 7 0.17850952041131052 1108 8 0.29102751552436956 1108 9 0.28478745179830867 1108 10-11 0.19351174621976241 1108 12-13 0.21257049516100324 1108 14-15 0.40755678322486233 1108 16-17 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2316 80-81 -3.091816670291472 2316 82-83 -2.8892890011921892 2316 84-85 -3.044928548780714 2316 86-87 -3.56884060634966 2316 88-89 -3.6579813530673277 2316 90-91 -3.3948607686030527 2316 92-93 -3.2645223936805756 2316 94-95 -3.8709424749373937 2316 96-97 -4.1058762197108365 2316 98-99 -4.107442683556638 2316 100-101 -4.590703678328335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1463.0 3 454.0 4 455.0 5 499.0 6 515.0 7 640.0 8 750.0 9 952.0 10 1253.0 11 1610.0 12 2179.0 13 2789.0 14 3237.0 15 4129.0 16 5631.0 17 7547.0 18 6896.0 19 4113.0 20 3815.0 21 3816.0 22 4190.0 23 4626.0 24 5054.0 25 5763.0 26 6828.0 27 8030.0 28 9429.0 29 11478.0 30 14071.0 31 18023.0 32 23372.0 33 31029.0 34 44309.0 35 64711.0 36 99730.0 37 150855.0 38 143146.0 39 28379.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.558527787311526 18.867170324179558 14.988566395540687 21.58573549296823 2 14.892048054604498 20.76214183716789 40.8855588084487 23.460251299778914 3 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21.355002753594317 22-23 21.32721768594506 26.083268269589237 30.934278612484935 21.655235431980767 24-25 21.796346122707714 26.144618575311696 30.82699721291087 21.232038089069718 26-27 21.286575816640866 26.01482841085488 31.486981906472565 21.211613866031694 28-29 20.71339294696096 27.035110855716006 31.069434855644346 21.18206134167869 30-31 21.03445306067611 26.23021300197396 30.903372272882297 21.831961664467634 32-33 21.113060085044467 26.341324279560947 31.02875969013611 21.51685594525848 34-35 21.85724393895446 26.27423367536125 30.55453166887252 21.313990716811777 36-37 21.501683441371668 25.92547938636891 31.249935411242646 21.322901761016773 38-39 21.153463248946746 26.604751783555123 30.973747033149497 21.268037934348634 40-41 21.248863229255658 26.73381651831234 30.785060765563426 21.23225948686858 42-43 20.934931764021805 26.767146145351006 30.06371998206002 22.234202108567164 44-45 21.321281806626732 26.44713047132538 30.080844892855545 22.150742829192342 46-47 21.986509416609085 26.92832602266159 29.779453556279545 21.305711004449783 48-49 21.722374779029924 26.875541660868286 29.602205636166477 21.799877923935313 50-51 20.9321487993331 27.297491879006675 30.148985349486907 21.621373972173327 52-53 21.583377655543398 27.67655626073034 28.85747906292284 21.88258702080342 54-55 21.10604348664811 27.115771488411827 29.329029680050706 22.449155344889355 56-57 21.734939260933377 27.163695746542732 28.93618780533181 22.165177187192082 58-59 22.299784560118887 27.017828390801192 28.836287048322056 21.84610000075786 60-61 21.72237495779675 27.112746415307548 29.99772619219877 21.167152434696927 62-63 21.222944751967436 27.282273033563868 29.79759980487563 21.69718240959306 64-65 21.04711623455944 27.873792675339814 29.268326290964986 21.81076479913576 66-67 21.410248901903365 27.324502626819395 28.6364309706313 22.62881750064594 68-69 21.12681491220273 27.304355375847294 29.287899115308896 22.28093059664108 70-71 21.763755438957926 27.204883779416882 28.849438620304753 22.18192216132044 72-73 21.554149957969877 26.757754902366088 29.59285901305001 22.095236126614026 74-75 20.970852513844072 28.085435351946437 28.86870987767881 22.075002256530677 76-77 21.275639859542355 27.31985259664732 28.76591035627071 22.638597187539624 78-79 21.475739047976095 27.347876305256307 28.904053323924096 22.272331322843502 80-81 21.703937543974263 27.53531206305694 28.55492914846537 22.205821244503433 82-83 21.46760047604707 27.318886071901854 29.223970224316854 21.98954322773422 84-85 21.281388216503675 27.53157339518129 28.87967416821183 22.307364220103203 86-87 21.19760734856268 27.80881940525482 28.7504039545862 22.2431692915963 88-89 21.21435742245502 27.476040760163684 28.298671759964556 23.010930057416736 90-91 21.368828156813514 26.97444893611304 28.76115615411805 22.895566752955396 92-93 21.769271426612395 27.326239653902316 28.842691077634235 22.061797841851057 94-95 21.318880913614155 27.18352143023005 28.879046311180872 22.61855134497492 96-97 20.55339237104166 27.40560283932325 29.512077461148824 22.52892732848627 98-99 20.80753133460896 27.808121357162157 28.48609587039355 22.898251437835334 100-101 20.831170550025956 27.288362605872983 28.710526188807016 23.169940655294045 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 169.0 1 134.0 2 123.5 3 169.0 4 167.0 5 176.5 6 283.0 7 589.0 8 1032.0 9 1285.5 10 1168.5 11 981.0 12 990.5 13 1096.0 14 1190.5 15 1243.5 16 1267.0 17 1322.5 18 1454.0 19 1588.5 20 1713.5 21 1932.5 22 2295.5 23 2746.0 24 3287.0 25 3968.5 26 4958.0 27 6072.5 28 7005.5 29 8189.0 30 9625.5 31 11217.0 32 12851.5 33 14339.0 34 16196.0 35 17625.0 36 19059.5 37 20675.0 38 21403.0 39 21805.5 40 22306.5 41 23066.5 42 23477.0 43 23770.5 44 24687.5 45 25517.5 46 26039.5 47 29471.0 48 37244.5 49 36381.0 50 28018.5 51 24014.0 52 22226.5 53 21103.0 54 19909.5 55 18236.0 56 16558.0 57 14730.5 58 13180.5 59 11608.5 60 9691.5 61 8004.5 62 6325.5 63 4766.0 64 3429.5 65 2459.0 66 1807.0 67 1329.0 68 924.5 69 626.0 70 429.0 71 302.5 72 227.5 73 189.0 74 143.5 75 86.0 76 58.5 77 34.5 78 21.0 79 17.0 80 10.5 81 7.5 82 6.0 83 4.5 84 4.5 85 4.0 86 3.0 87 2.0 88 0.5 89 1.5 90 2.0 91 2.0 92 1.5 93 0.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039681391494128394 2 0.0376146523538092 3 0.02287191315286567 4 0.04367708716541215 5 0.06365556552183096 6 0.06034878289732027 7 0.0631044350844125 8 0.0673756959744055 9 0.0631044350844125 10-11 0.06868463076327433 12-13 0.07082026120827081 14-15 0.06331110899844444 16-17 0.05373521764829887 18-19 0.044090434993475994 20-21 0.05035954371911086 22-23 0.029003239269145928 24-25 0.01563832616174852 26-27 0.010953717443691691 28-29 0.014880521810298149 30-31 0.011504847881110142 32-33 0.005924652202248337 34-35 0.008335847865954055 36-37 0.004891282632088743 38-39 0.007371369600471768 40-41 0.0056490869835391115 42-43 0.003789021757251843 44-45 0.0011711521795142062 46-47 0.0021356304449964936 48-49 5.511304374184499E-4 50-51 0.00530463046015258 52-53 0.004822391327411437 54-55 0.0056490869835391115 56-57 0.0187384348722273 58-59 0.007784717428535605 60-61 0.017360608778681175 62-63 0.012400434841915125 64-65 0.007509152209826379 66-67 0.012882673974656266 68-69 0.022114108801415303 70-71 0.01563832616174852 72-73 0.015293869638361986 74-75 0.015087195724330068 76-77 0.02108073923125571 78-79 0.034859000166716954 80-81 0.029554369706564377 82-83 0.030656630581401275 84-85 0.01798063052077693 86-87 0.01963402183303228 88-89 0.016740587036585416 90-91 0.016051673989812353 92-93 0.028314326222372864 94-95 0.018669543567549993 96-97 0.035272347994780795 98-99 0.053046304601525804 100-101 0.06096880463941603 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 725781.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.8555215389025 #Duplication Level Percentage of deduplicated Percentage of total 1 86.87301998368939 61.5543313860381 2 8.382544243757966 11.878990884287912 3 2.076492636671635 4.413929062291784 4 0.8742926418638858 2.4779384446756207 5 0.4774043071541872 1.6913365584164164 6 0.2895866041477384 1.2311285920540624 7 0.19809002867902883 0.9825040605596117 8 0.13408730498844873 0.7600660741361932 9 0.09887904677347682 0.630551378556381 >10 0.5541399563695942 6.8984348133196125 >50 0.026669094053627277 1.2801298483133101 >100 0.010316974320392377 1.3725143855231337 >500 0.0023359187116399243 1.1551409880462267 >1k 0.002141258819003264 3.6730035237816137 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4243 0.5846116114916208 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4198 0.5784113940706632 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3487 0.4804479588195337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3144 0.43318852381090167 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2343 0.32282465371785707 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2081 0.2867256100669486 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1969 0.271293957819232 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1467 0.2021270879232165 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1450 0.19978478356418808 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1132 0.15596991378942132 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1116 0.1537653920397475 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 992 0.1366803484797756 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 894 0.12317765276302355 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 874 0.12042200057593132 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 825 0.11367065271755529 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 738 0.101683565703704 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3778260935461248E-4 0.0 10-11 0.0 0.0 0.0 1.3778260935461248E-4 0.0 12-13 0.0 0.0 0.0 1.3778260935461248E-4 0.0 14-15 0.0 0.0 0.0 2.7556521870922496E-4 0.0 16-17 0.0 0.0 0.0 4.1334782806383744E-4 0.0 18-19 0.0 0.0 0.0 4.1334782806383744E-4 0.0 20-21 0.0 0.0 0.0 4.1334782806383744E-4 0.0 22-23 0.0 0.0 0.0 6.200217420957562E-4 0.0 24-25 0.0 0.0 0.0 8.955869608049811E-4 0.0 26-27 0.0 0.0 0.0 0.0013089347888688187 0.0 28-29 0.0 0.0 0.0 0.002273413054351106 0.0 30-31 0.0 0.0 0.0 0.0042023695853156805 0.0 32-33 0.0 0.0 0.0 0.00695802177240793 0.0 34-35 0.0 0.0 0.0 0.009300326131436342 0.0 36-37 0.0 0.0 0.0 0.013089347888688185 0.0 38-39 0.0 0.0 0.0 0.016120565294489658 0.0 40-41 6.889130467730624E-5 0.0 0.0 0.020736282707869178 0.0 42-43 1.3778260935461248E-4 0.0 0.0 0.028658782745759392 0.0 44-45 1.3778260935461248E-4 0.0 0.0 0.03919915236138725 0.0 46-47 1.3778260935461248E-4 0.0 0.0 0.06820239163053318 0.0 48-49 1.3778260935461248E-4 0.0 0.0 0.12917119626994922 0.0 50-51 1.3778260935461248E-4 0.0 0.0 0.1666480660144038 0.0 52-53 1.3778260935461248E-4 0.0 0.0 0.1976491531191916 0.0 54-55 1.3778260935461248E-4 0.0 0.0 0.24663087074475634 0.0 56-57 1.3778260935461248E-4 0.0 0.0 0.2833499361377606 0.0 58-59 1.3778260935461248E-4 0.0 0.0 0.3253736319909174 0.0 60-61 1.3778260935461248E-4 0.0 0.0 0.40404750193240113 0.0 62-63 1.3778260935461248E-4 0.0 0.0 0.44917130649603665 0.0 64-65 1.3778260935461248E-4 0.0 0.0 0.5088311763465838 0.0 66-67 1.3778260935461248E-4 0.0 0.0 0.6304243291020295 0.0 68-69 1.3778260935461248E-4 0.0 0.0 0.7573221123176275 0.0 70-71 1.3778260935461248E-4 0.0 0.0 0.8652058954422891 0.0 72-73 1.3778260935461248E-4 0.0 0.0 0.994308200407561 0.0 74-75 1.3778260935461248E-4 0.0 0.0 1.0987474182983572 0.0 76-77 1.3778260935461248E-4 0.0 0.0 1.171358853428238 0.0 78-79 1.3778260935461248E-4 0.0 0.0 1.2733179843506512 0.0 80-81 1.3778260935461248E-4 0.0 0.0 1.4153718545952567 0.0 82-83 1.3778260935461248E-4 0.0 0.0 1.5753374640559619 0.0 84-85 1.3778260935461248E-4 0.0 0.0 1.7561771388338907 0.0 86-87 1.3778260935461248E-4 0.0 0.0 2.1210254884049045 0.0 88-89 1.3778260935461248E-4 0.0 0.0 2.5952043384988035 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGA 45 5.694965E-7 36.948063 76-77 ACGCGAA 60 5.3788553E-6 27.711046 78-79 TAACGCG 60 5.3788553E-6 27.711046 76-77 GCACCGT 105 6.884847E-5 27.153002 6 TATAGGG 195 5.329639E-10 26.80304 2 GTGTAGG 315 0.0 25.642735 1 GTATAGG 260 0.0 25.584719 1 ATAACGC 65 1.0015823E-5 25.579428 74-75 TACATGG 3360 0.0 24.181473 2 GTACATG 3455 0.0 23.929142 1 TGCACCG 180 1.9406616E-7 23.760515 5 GAGTACT 3135 0.0 23.491196 12-13 GTACTAG 145 2.3273196E-5 22.938023 1 ACATGGG 3415 0.0 22.81808 3 GTACTTT 3455 0.0 21.04332 14-15 GCGTATT 80 4.982712E-5 20.785435 94-95 CTTTGCG 140 4.8394082E-4 20.363348 2 TTTGCGT 140 4.8394082E-4 20.363348 3 AGTACTT 3270 0.0 20.19659 12-13 GTATACA 355 3.6379788E-12 20.07654 1 >>END_MODULE