FastQCFastQC Report
Thu 26 May 2016
SRR936922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725781
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66900.9217656565823575No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.7226697860649425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43290.5964609158961174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.3777999148503474No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23540.3243402624207578No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17040.23478156634025968No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16070.22141665323286228No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15370.2117718705780394No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15330.21122074014062092No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14900.2052960879383726No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14800.20391826184482648No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13630.18779769655033682No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12210.16823256602198183No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.15955226163264125No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10040.13833373979203092No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7510.10347473962531398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG300.00394743431.67298576-77
TTGCGTA951.19252945E-824.99809660-61
GAGTACT44200.024.49955212-13
GTACTTT46400.023.08283214-15
AGTACTT44650.022.07202512-13
TATAGGG1754.043679E-621.7156242
GGTGTGC4650.021.4485028
GTACATG36950.020.9567281
AGAGTAC71600.020.72848710-11
ACTTTTT53450.020.48253816-17
GAACGAT1404.8541135E-420.354196
TACATGG37200.020.0482792
TACTTTT50300.019.73507914-15
GTAGGAC1951.0324657E-519.4856973
ACATGGG34850.019.3529013
TATCTCG1503.7252903E-918.99724688-89
TATACTG3557.8216544E-1118.7296775
CATGGGA22150.017.58214
GTCTATA1650.001461829517.2749821
GCGTAAG1254.5651977E-617.09869662-63
Produced by FastQC (version 0.11.5)