##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936922_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 725781 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.434945252080176 33.0 31.0 34.0 31.0 34.0 2 32.50895104721672 34.0 31.0 34.0 31.0 34.0 3 32.63066131519012 34.0 31.0 34.0 31.0 34.0 4 36.24427203247261 37.0 35.0 37.0 35.0 37.0 5 36.19176859135194 37.0 35.0 37.0 35.0 37.0 6 36.247993540751274 37.0 37.0 37.0 35.0 37.0 7 36.22164675019049 37.0 37.0 37.0 35.0 37.0 8 36.20621096446449 37.0 36.0 37.0 35.0 37.0 9 37.9419948992878 39.0 38.0 39.0 35.0 39.0 10-11 37.85039702058886 39.0 38.0 39.0 35.0 39.0 12-13 37.792099131831776 39.0 37.0 39.0 35.0 39.0 14-15 39.031789892543344 40.0 38.0 41.0 36.0 41.0 16-17 38.99168550843849 40.0 38.0 41.0 36.0 41.0 18-19 39.06536131422564 40.0 38.5 41.0 36.0 41.0 20-21 39.1501031302831 40.0 39.0 41.0 36.0 41.0 22-23 39.089570407602295 40.0 38.5 41.0 36.0 41.0 24-25 38.996334982591165 40.0 38.0 41.0 35.0 41.0 26-27 38.81484497389708 40.0 38.0 41.0 35.0 41.0 28-29 38.679065723682484 40.0 38.0 41.0 35.0 41.0 30-31 38.62153183949428 40.0 38.0 41.0 35.0 41.0 32-33 38.4952499445425 40.0 38.0 41.0 35.0 41.0 34-35 38.338742678576594 40.0 38.0 41.0 34.0 41.0 36-37 38.162626880560396 40.0 38.0 41.0 34.0 41.0 38-39 38.010956473095874 40.0 38.0 41.0 34.0 41.0 40-41 37.81125780366254 40.0 37.0 41.0 33.0 41.0 42-43 37.56105147420503 40.0 37.0 41.0 33.0 41.0 44-45 37.23863327918477 40.0 37.0 41.0 33.0 41.0 46-47 36.786088365498685 40.0 37.0 41.0 31.5 41.0 48-49 36.314806394766464 40.0 36.0 41.0 30.0 41.0 50-51 36.03505671821114 39.0 36.0 41.0 29.5 41.0 52-53 35.83213118006671 39.0 35.5 41.0 29.0 41.0 54-55 35.58247253648139 39.0 35.0 40.0 28.0 41.0 56-57 35.31041112952805 39.0 35.0 40.0 27.5 41.0 58-59 34.97876632207236 38.0 35.0 40.0 26.5 41.0 60-61 34.621277630580025 38.0 34.0 40.0 26.0 41.0 62-63 34.32187602045245 38.0 34.0 40.0 26.0 41.0 64-65 33.9471410797472 37.0 34.0 40.0 25.5 41.0 66-67 33.51389399832732 36.5 33.0 39.0 23.5 40.5 68-69 33.05667343179279 36.0 33.0 39.0 22.5 40.0 70-71 32.64092543067399 35.0 32.0 38.5 22.0 40.0 72-73 32.24253941615997 35.0 32.0 38.0 20.5 40.0 74-75 31.777318502413262 35.0 31.5 37.0 19.0 39.0 76-77 30.36292077086614 33.5 29.5 35.5 18.0 38.5 78-79 31.010295805484024 34.5 31.0 36.0 17.5 38.5 80-81 30.610573299659265 34.0 31.0 36.0 9.5 37.5 82-83 30.471886836387284 34.0 31.0 35.0 9.5 37.0 84-85 30.157466232927014 34.0 31.0 35.0 7.0 37.0 86-87 29.838183281182616 34.0 30.5 35.0 4.5 36.0 88-89 29.472883004652918 34.0 30.0 35.0 2.0 36.0 90-91 29.03827669779176 34.0 29.5 35.0 2.0 35.5 92-93 28.660808839029954 34.0 29.0 35.0 2.0 35.0 94-95 28.196699830940737 34.0 29.0 35.0 2.0 35.0 96-97 27.60956886443707 33.5 27.5 35.0 2.0 35.0 98-99 26.935034121863204 33.0 26.5 35.0 2.0 35.0 100-101 23.83687434639375 30.0 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11338601554796668 1101 2 0.07780220766193224 1101 3 -0.0033304100772824086 1101 4 -0.0019792737132462435 1101 5 0.01083875363440967 1101 6 0.02750819512834113 1101 7 0.006088734625500081 1101 8 0.038292734649552074 1101 9 0.08257443117425112 1101 10-11 -0.022698307054533018 1101 12-13 0.009173819722228416 1101 14-15 -0.023490604115480096 1101 16-17 -0.0461441523574635 1101 18-19 -0.13512839466703497 1101 20-21 -0.05262993905700597 1101 22-23 0.07323109438797104 1101 24-25 0.05850176789024175 1101 26-27 -0.05297816969134317 1101 28-29 0.019772981973787296 1101 30-31 -0.14835705613858607 1101 32-33 0.008248286326463017 1101 34-35 0.05790495922658323 1101 36-37 0.0624301934147411 1101 38-39 0.03364130391207709 1101 40-41 0.21678406523475502 1101 42-43 0.18386619691626294 1101 44-45 0.02475722581451123 1101 46-47 0.10437568099377614 1101 48-49 0.13556641486516696 1101 50-51 0.07282655495918533 1101 52-53 0.23925934094707912 1101 54-55 0.38801421011948634 1101 56-57 0.3772362069091031 1101 58-59 0.34514899845513014 1101 60-61 -4.285591932529087E-5 1101 62-63 0.0826629588036738 1101 64-65 0.14576773604668603 1101 66-67 0.07734415037828057 1101 68-69 -0.051666468489237616 1101 70-71 0.15529530427049565 1101 72-73 0.07063413267047736 1101 74-75 0.16888362727571504 1101 76-77 -0.04925383535415051 1101 78-79 0.12462596320724728 1101 80-81 0.30749097684847015 1101 82-83 0.2086830986665973 1101 84-85 0.07774747852500852 1101 86-87 0.24150670237721528 1101 88-89 -0.11087946484530065 1101 90-91 -0.13545732006746647 1101 92-93 -0.05249166650886394 1101 94-95 0.0389545842457224 1101 96-97 -0.02511563312172882 1101 98-99 -0.09144819693273121 1101 100-101 -0.07142194356566733 1102 1 0.1737165940603589 1102 2 0.12573609195945323 1102 3 0.07683487917891796 1102 4 0.1071116353776631 1102 5 0.13232635694019024 1102 6 0.08866521992172949 1102 7 0.07633666850979637 1102 8 0.14738364374046142 1102 9 0.0685248443973876 1102 10-11 0.07936780864795168 1102 12-13 0.13396720815197938 1102 14-15 -0.0028294470906828906 1102 16-17 0.06831865755989242 1102 18-19 0.16404515905198025 1102 20-21 -0.019158864676846576 1102 22-23 0.03934679686731357 1102 24-25 -0.07910153789488561 1102 26-27 0.06107141708551467 1102 28-29 -0.02774767918323562 1102 30-31 0.09461815047298217 1102 32-33 0.08924002186365243 1102 34-35 0.001706612119143358 1102 36-37 0.061190523993253976 1102 38-39 0.26669915515174836 1102 40-41 0.2903377842430217 1102 42-43 0.2578331390650277 1102 44-45 0.5020299530872379 1102 46-47 0.5828880776879899 1102 48-49 0.5640788115593836 1102 50-51 0.67613234008315 1102 52-53 0.7537221508644265 1102 54-55 0.64462578036742 1102 56-57 0.5735171986446446 1102 58-59 0.41374403977744123 1102 60-61 0.4334282184608398 1102 62-63 0.34795221500201734 1102 64-65 0.6870900500962804 1102 66-67 0.4785838197997663 1102 68-69 0.4301517133289394 1102 70-71 0.534220924105206 1102 72-73 0.6202209095299764 1102 74-75 0.6081398256228248 1102 76-77 0.6073577348937818 1102 78-79 0.70768381444692 1102 80-81 0.6446810594930987 1102 82-83 0.6880219416418036 1102 84-85 0.7624582223266607 1102 86-87 0.7154736445259751 1102 88-89 0.5188726012704024 1102 90-91 0.6905757377837745 1102 92-93 0.634698416135766 1102 94-95 0.7373016916837365 1102 96-97 0.7662066809278549 1102 98-99 0.7197088278606572 1102 100-101 0.9984127671781344 1103 1 0.08734307985272949 1103 2 0.07424453751606563 1103 3 0.09443225825764756 1103 4 0.08228861341592619 1103 5 0.18796825216919189 1103 6 0.21826747207015984 1103 7 0.16836360209775592 1103 8 0.1391125957012136 1103 9 0.22129722594557677 1103 10-11 0.07296449627759927 1103 12-13 0.15150038124473753 1103 14-15 0.183828878285496 1103 16-17 0.2261781287659801 1103 18-19 0.33648185853503776 1103 20-21 0.23228823946855925 1103 22-23 0.18237633162085842 1103 24-25 0.1846591219450957 1103 26-27 0.18201820843253103 1103 28-29 0.2015014400423425 1103 30-31 0.31115530762948396 1103 32-33 0.41004440762638694 1103 34-35 0.2650384088989668 1103 36-37 0.27875333061799523 1103 38-39 0.4285715004811763 1103 40-41 0.3370254138897266 1103 42-43 0.28272365962740054 1103 44-45 0.5033198348823689 1103 46-47 0.5931248166824261 1103 48-49 0.4947519866277048 1103 50-51 0.4257973165409581 1103 52-53 0.3991915130849719 1103 54-55 0.4647444791261037 1103 56-57 0.3407902551788382 1103 58-59 0.5799000767864371 1103 60-61 0.5511787041215044 1103 62-63 0.45487905310415755 1103 64-65 0.29520222947792263 1103 66-67 0.3010666158881463 1103 68-69 0.3677797137240546 1103 70-71 0.32842016021859877 1103 72-73 0.44137266613989823 1103 74-75 0.37294424431170725 1103 76-77 0.442511335367918 1103 78-79 0.6117238526412478 1103 80-81 1.060922704483385 1103 82-83 0.8031095251918998 1103 84-85 0.6100735461553093 1103 86-87 0.5931019741825523 1103 88-89 0.2878685184282155 1103 90-91 0.54888300458434 1103 92-93 0.5859660727819076 1103 94-95 0.6816046451590942 1103 96-97 0.7987286591307488 1103 98-99 0.9541783876380734 1103 100-101 1.2619849248285249 1104 1 0.07540525954682664 1104 2 0.040224126374837965 1104 3 0.05019964376555919 1104 4 0.09336749434061886 1104 5 0.033876517001097284 1104 6 0.03281726402332197 1104 7 -0.033993451375366135 1104 8 0.07750994968151304 1104 9 0.03142740445462522 1104 10-11 0.0941920168280248 1104 12-13 0.06588170168992491 1104 14-15 -0.007704654619509199 1104 16-17 0.10298720765575098 1104 18-19 0.07296821057416025 1104 20-21 0.08814339818994199 1104 22-23 0.05921636328111646 1104 24-25 0.09678475924052776 1104 26-27 0.018780482311484548 1104 28-29 -0.009435610866120214 1104 30-31 0.026754619593610585 1104 32-33 0.16485756343821123 1104 34-35 0.10473401051027764 1104 36-37 0.03638429400086807 1104 38-39 0.11828600539160306 1104 40-41 0.13197104755926148 1104 42-43 -0.007999131781879498 1104 44-45 -0.14647996701989996 1104 46-47 -0.1988360855466098 1104 48-49 -0.03182151956947621 1104 50-51 0.02296414313027384 1104 52-53 0.05652665641840571 1104 54-55 0.12725481514053172 1104 56-57 -0.006656804529065141 1104 58-59 0.16705257666483675 1104 60-61 0.03118874166278829 1104 62-63 0.12961904983200867 1104 64-65 0.05120430331354697 1104 66-67 0.013444076080673995 1104 68-69 0.1189061734788126 1104 70-71 0.08173929212604492 1104 72-73 0.268006198602734 1104 74-75 -0.069667661008193 1104 76-77 0.10956785954866888 1104 78-79 0.20350434443655274 1104 80-81 0.09957552936587177 1104 82-83 -0.1481912649881103 1104 84-85 -0.04136122530269759 1104 86-87 -0.0015538089004323297 1104 88-89 0.08697232517680575 1104 90-91 0.1143945433349991 1104 92-93 -0.02500292580753083 1104 94-95 -0.11051157365046294 1104 96-97 0.08749202962214397 1104 98-99 0.3890885803853976 1104 100-101 0.2835844313529279 1105 1 0.07355546302444793 1105 2 0.13885527694827715 1105 3 0.03458736111664962 1105 4 0.03180626607572634 1105 5 0.10550063834774903 1105 6 0.1883481556252491 1105 7 0.09475238658246354 1105 8 -0.013056088054334225 1105 9 -0.019774963284923786 1105 10-11 -0.01635182334955232 1105 12-13 0.16162716968844393 1105 14-15 0.20894351408193046 1105 16-17 0.15333866899499782 1105 18-19 0.11614246140656803 1105 20-21 0.12151372745372413 1105 22-23 -0.1466659896600575 1105 24-25 -0.0012544564457428464 1105 26-27 0.14656442607767417 1105 28-29 0.08708677167179957 1105 30-31 0.033598866726428867 1105 32-33 0.0060340340264488646 1105 34-35 -0.03266887517229833 1105 36-37 0.17301026514404327 1105 38-39 0.05561489921328899 1105 40-41 0.21850452887631633 1105 42-43 0.055041933700920254 1105 44-45 0.08270982210330402 1105 46-47 -0.06505880104790407 1105 48-49 -0.0023357630027049936 1105 50-51 0.0026903732449596873 1105 52-53 0.01782891317016322 1105 54-55 -0.13123575089720418 1105 56-57 0.005033909492397015 1105 58-59 -0.04412019433035397 1105 60-61 0.1312997294541347 1105 62-63 0.14097680047944294 1105 64-65 0.2320404113416643 1105 66-67 0.21100234467112244 1105 68-69 0.4049217117696102 1105 70-71 0.3663119891293718 1105 72-73 0.4732226247958522 1105 74-75 0.4711758462060125 1105 76-77 0.4225534311357926 1105 78-79 0.39802894673029954 1105 80-81 0.5663531838238853 1105 82-83 0.2898775474425186 1105 84-85 0.2450612682230151 1105 86-87 0.08882724918318274 1105 88-89 0.19618431185827134 1105 90-91 0.24332536094506096 1105 92-93 0.08535593471781056 1105 94-95 0.3043607903894916 1105 96-97 0.13321899965544048 1105 98-99 0.07447518785857454 1105 100-101 0.20522705066480285 1106 1 0.07041080893639418 1106 2 0.03489790260798031 1106 3 0.1121549332413565 1106 4 0.04824783283849854 1106 5 0.0547157394319413 1106 6 0.037310335469108225 1106 7 0.11860285954517735 1106 8 0.1282712426148933 1106 9 0.030144944988975908 1106 10-11 0.059253923021024946 1106 12-13 -0.11409407738188548 1106 14-15 0.08184806160583236 1106 16-17 0.16734376114263938 1106 18-19 0.1288846334941809 1106 20-21 0.1461769206686867 1106 22-23 0.11028934444890837 1106 24-25 0.1051073558192499 1106 26-27 -0.06154507195797265 1106 28-29 -0.046077947501316885 1106 30-31 -0.1236844219241604 1106 32-33 -0.10415616274627837 1106 34-35 0.007593657075162241 1106 36-37 0.14371439787710472 1106 38-39 0.2865083856482755 1106 40-41 0.2866398248784776 1106 42-43 0.04451029325375089 1106 44-45 -0.1841220864403752 1106 46-47 -0.1619006578270401 1106 48-49 -0.2113338576626944 1106 50-51 -0.4414401678378752 1106 52-53 -0.1796326006477713 1106 54-55 -0.03018865696225248 1106 56-57 -0.07220655116148578 1106 58-59 0.12552588454735059 1106 60-61 -0.14150364957364303 1106 62-63 -0.08373191855879014 1106 64-65 0.0793484862737941 1106 66-67 0.1090371466337956 1106 68-69 0.215405297181114 1106 70-71 0.2544145739844481 1106 72-73 0.26044757294699394 1106 74-75 0.13507133914951197 1106 76-77 -0.11258997520747016 1106 78-79 0.10002533838727246 1106 80-81 0.30311612917603625 1106 82-83 0.0729697625267427 1106 84-85 0.01524775548421431 1106 86-87 0.21941975934218405 1106 88-89 0.04904232187395152 1106 90-91 0.15152338141481891 1106 92-93 0.36863699766147917 1106 94-95 0.4304951421523562 1106 96-97 0.38434196996248815 1106 98-99 0.452556190101145 1106 100-101 0.6194184614594818 1107 1 0.11574233802976153 1107 2 0.051977718459411903 1107 3 0.0378588226361245 1107 4 0.07107231487530896 1107 5 0.054256819146210944 1107 6 0.021057664480487404 1107 7 0.12033514758024211 1107 8 0.05700799650664834 1107 9 0.013652837329537704 1107 10-11 0.05357183687461742 1107 12-13 0.13388612918665643 1107 14-15 0.12678506436660797 1107 16-17 0.16253688858462567 1107 18-19 0.2280785300357735 1107 20-21 0.15992276227044755 1107 22-23 0.08115334804771379 1107 24-25 0.06481306638384154 1107 26-27 0.17556162610149784 1107 28-29 0.18050895531278854 1107 30-31 0.09020689559425676 1107 32-33 -0.08581114175929372 1107 34-35 -0.1613588437683049 1107 36-37 -0.38156884482097553 1107 38-39 -0.1970484823749601 1107 40-41 -0.02011301480416705 1107 42-43 -0.17352037640708318 1107 44-45 -0.16384978164474973 1107 46-47 -0.09800915891720052 1107 48-49 -0.15491659210202613 1107 50-51 -0.4328540051007579 1107 52-53 -0.3465535176176573 1107 54-55 -0.5258715412308987 1107 56-57 -0.6472899005177268 1107 58-59 -0.4543371286543234 1107 60-61 -0.5986617176824822 1107 62-63 -0.4307536528784084 1107 64-65 -0.4509801586960407 1107 66-67 -0.5198169675049442 1107 68-69 -0.48654048855209453 1107 70-71 -0.617508574099876 1107 72-73 -0.5470444367610767 1107 74-75 -0.4464486846490452 1107 76-77 -0.267517291666568 1107 78-79 -0.5556701707240173 1107 80-81 -0.452301125501144 1107 82-83 -0.4215095896882275 1107 84-85 -0.3623284561197586 1107 86-87 -0.5483511578091012 1107 88-89 -0.6264801761035379 1107 90-91 -0.7892232982152869 1107 92-93 -0.6149878921432368 1107 94-95 -0.8536191416961891 1107 96-97 -0.7569410840885276 1107 98-99 -0.7211197432483942 1107 100-101 -0.9835619809179086 1108 1 0.10852740086464507 1108 2 0.09594696547240034 1108 3 0.03300234264357016 1108 4 0.12080420724389995 1108 5 0.011770204300873388 1108 6 0.05357646033235852 1108 7 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66-67 -0.5101854518642455 2316 68-69 -0.698807737640081 2316 70-71 -0.4772635377381107 2316 72-73 -0.6160496912143039 2316 74-75 -0.7304433748024799 2316 76-77 -0.624381910362608 2316 78-79 -0.9286206174316796 2316 80-81 -1.0283808998128805 2316 82-83 -0.735207965080928 2316 84-85 -0.8983459920660728 2316 86-87 -0.9998216529463555 2316 88-89 -0.6474050073517041 2316 90-91 -0.9091765699828898 2316 92-93 -1.1357960017270763 2316 94-95 -1.125383775427565 2316 96-97 -0.8833704298932723 2316 98-99 -0.8923652623234801 2316 100-101 -1.9211742513323316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 2.0 5 8.0 6 25.0 7 51.0 8 136.0 9 279.0 10 517.0 11 933.0 12 1582.0 13 2315.0 14 2994.0 15 4350.0 16 6164.0 17 9703.0 18 8903.0 19 4727.0 20 3555.0 21 3457.0 22 3546.0 23 3525.0 24 4020.0 25 4595.0 26 5500.0 27 6852.0 28 8363.0 29 10562.0 30 13644.0 31 17728.0 32 22746.0 33 31135.0 34 44958.0 35 67290.0 36 109233.0 37 171616.0 38 138459.0 39 12306.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4791765729037 19.669548606373347 15.30942605157721 20.54184876914574 2 15.544348449147755 21.91779311865294 39.661582131095585 22.876276301103715 3 20.748019506602564 25.7225063145667 30.294762457920115 23.23471172091062 4 12.914501757417183 17.74764012835828 38.39587974884986 30.941978365374677 5 13.08975766903711 38.39279193874416 33.88568680648659 14.631763585732138 6 29.992931752140105 37.86472778978783 17.1762556473647 14.966084810707361 7 26.920517346141605 34.47610229532049 20.853535708429956 17.749844650107953 8 25.990107195017774 33.91620050152939 19.664223318360936 20.4294689850919 9 26.23987125592982 17.899889911695123 20.831352708323863 35.0288861240512 10-11 24.72281583563086 27.2914970218289 26.888345106857305 21.09734203568294 12-13 26.992508759529386 25.044055989341135 27.330558391580933 20.632876859548542 14-15 23.03229211015444 26.191647342655706 27.68934430634034 23.086716240849512 16-17 20.92421818702887 29.32337716198137 27.965736220705693 21.786668430284067 18-19 20.672652629301822 28.007512192500624 31.084727803586205 20.235107374611353 20-21 22.254119225901743 26.00466950088423 31.08228669137134 20.658924581842683 22-23 21.502560689794855 26.265361038660423 31.181168975214284 21.050909296330435 24-25 22.015851341315038 26.326674869754168 31.321900452363995 20.3355733365668 26-27 21.544604823591744 26.03467744546814 32.00936140837249 20.411356322567624 28-29 20.791025300077578 27.408922614364318 31.48709780096978 20.312954284588326 30-31 21.29180173326277 26.36589540883832 31.237527013319223 21.104775844579695 32-33 21.34893772686846 26.637741404574538 31.219442295411064 20.79387857314594 34-35 21.995723086377396 26.342288121230663 31.170419300123868 20.491569492268074 36-37 21.830565230422604 25.969869535709734 31.72387183011737 20.475693403750288 38-39 21.19613519647141 26.797744061488515 31.528297753018 20.477822989022073 40-41 21.413133568639715 27.005782529224405 31.022962697071392 20.558121205064484 42-43 21.144056323705605 27.28081313595142 30.24974043502195 21.32539010532103 44-45 21.810931782968165 26.57191141203517 30.14462680387371 21.47253000112295 46-47 22.449949708585365 27.419154828664727 29.61165382972567 20.519241633024237 48-49 22.374297731038308 27.516680502499064 29.231818510621444 20.877203255841188 50-51 21.405472585403224 27.914140015404477 29.846520145006018 20.833867254186284 52-53 22.14246740685014 28.451939054736652 28.374090271520018 21.031503266893186 54-55 21.456216905883537 27.825305778268905 28.872192468423236 21.846284847424325 56-57 22.229670194157837 27.774084410399634 28.751452756396432 21.244792639046096 58-59 22.879225548841692 27.46953788242556 28.403913866282892 21.247322702449853 60-61 21.901234669962427 27.833327133115915 29.73844727266214 20.526990924259522 62-63 21.62449829907369 27.78682550245873 29.563187793563074 21.02548840490451 64-65 21.45917601757675 28.647922819940984 28.880584623927717 21.012316538554547 66-67 21.8695424831198 27.774046142304137 28.470128200623744 21.88628317395231 68-69 21.618875258258235 28.019339313603176 28.64859725906978 21.713188169068808 70-71 22.384619147251946 27.89284880184138 28.372553166717076 21.349978884189596 72-73 22.154469864210494 27.438189179282386 29.071204742573194 21.336136213933926 74-75 21.580570775831006 28.94534686726427 28.23571803070419 21.23836432620053 76-77 21.91357833319899 28.090314162276414 28.13316605936848 21.862941445156117 78-79 21.987129579230942 28.202688457271996 28.03546944653057 21.774712516966492 80-81 22.651529869202967 28.16586546079327 27.802877176448543 21.37972749355522 82-83 22.135758452608552 28.06979107459653 28.454227596066072 21.340222876728845 84-85 21.84701488681433 28.423832956840016 28.197523617807963 21.53162853853769 86-87 21.768343343239906 28.60160296287723 27.849723263629112 21.780330430253755 88-89 21.54790716387413 28.527288472229973 27.520569433852298 22.4042349300436 90-91 21.926132805530084 27.87893777070182 27.814110561737067 22.380818862031028 92-93 22.332111659180466 28.264763426933783 27.866846702857618 21.536278211028133 94-95 21.95953859169057 28.202287751902094 27.791073854539544 22.04709980186779 96-97 21.17384055774477 28.269654716305215 28.75795695665353 21.79854776929648 98-99 21.61176829633753 28.88028818630426 27.252247752591863 22.255695764766344 100-101 21.142390253748918 28.825609536820217 27.202175876457837 22.82982433297303 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 353.0 1 259.0 2 185.0 3 229.0 4 224.0 5 196.0 6 333.0 7 749.5 8 1252.5 9 1517.5 10 1427.5 11 1294.0 12 1301.5 13 1375.0 14 1509.0 15 1608.0 16 1717.0 17 1787.0 18 1812.0 19 1947.0 20 2092.0 21 2323.0 22 2665.0 23 3035.5 24 3502.0 25 4139.5 26 5040.5 27 6158.5 28 7123.5 29 8177.5 30 9703.5 31 11316.5 32 12591.5 33 13933.5 34 15781.0 35 17132.5 36 18720.0 37 20247.0 38 20994.0 39 21534.0 40 21978.0 41 22734.5 42 23145.5 43 23507.0 44 24376.5 45 24809.0 46 25348.5 47 29646.0 48 37823.5 49 36185.0 50 27328.0 51 23431.0 52 21823.5 53 21040.5 54 19927.5 55 18141.5 56 16221.5 57 14521.0 58 13190.5 59 11904.5 60 10029.5 61 8093.0 62 6390.0 63 4803.0 64 3441.0 65 2474.5 66 1837.5 67 1367.5 68 971.0 69 610.5 70 436.5 71 326.5 72 227.5 73 169.5 74 131.0 75 104.5 76 80.5 77 42.0 78 18.0 79 9.5 80 6.5 81 5.0 82 3.5 83 3.0 84 1.5 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03141443493285165 2 0.007026913077085236 3 0.010747043529659772 4 0.0 5 0.005235739155475274 6 0.0 7 0.0 8 1.3778260935461248E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.003789021757251843 20-21 0.0010333695701595935 22-23 0.0 24-25 0.005235739155475274 26-27 0.004891282632088743 28-29 0.007164695686439849 30-31 0.02934769579253246 32-33 3.444565233865312E-4 34-35 0.0033067826245106995 36-37 0.01426050006820239 38-39 0.007164695686439849 40-41 0.008335847865954055 42-43 0.0065446739443440925 44-45 0.002273413054351106 46-47 0.0015156087029007373 48-49 0.0011711521795142062 50-51 0.0024800869683830246 52-53 0.002066739140319187 54-55 0.0013778260935461248 56-57 7.578043514503686E-4 58-59 3.444565233865312E-4 60-61 0.0 62-63 0.0 64-65 0.006820239163053317 66-67 6.200217420957562E-4 68-69 7.578043514503686E-4 70-71 0.0029623261011241684 72-73 0.013640478326106634 74-75 0.006820239163053317 76-77 0.003513456538542618 78-79 0.011849304404496674 80-81 0.0 82-83 0.005993543506925643 84-85 6.200217420957562E-4 86-87 0.0 88-89 0.0010333695701595935 90-91 7.578043514503686E-4 92-93 1.3778260935461248E-4 94-95 4.1334782806383744E-4 96-97 1.3778260935461248E-4 98-99 6.889130467730624E-5 100-101 6.200217420957562E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 725781.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.74406068677729 #Duplication Level Percentage of deduplicated Percentage of total 1 85.64478640230527 58.01925607543846 2 9.089310255010979 12.314935710328216 3 2.2785291636994307 4.630704538267384 4 0.9745957584240403 2.640922968150157 5 0.5323092034657589 1.8030393491857226 6 0.3201354784066039 1.3012366366300472 7 0.21308331738391492 1.010459042893703 8 0.15772084505244324 0.8547720399042009 9 0.11766509147576468 0.7173999987884463 >10 0.6168270959943999 7.496685124141425 >50 0.032207316304137625 1.4844839163185222 >100 0.01895711375634457 2.4827101813875063 >500 8.153597307202721E-4 0.34715687151445956 >1k 0.0026499191248408847 3.2481399887681457 >5k 4.0767986536013607E-4 1.6480975582836417 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6690 0.9217656565823575 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.7226697860649425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4329 0.5964609158961174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2742 0.3777999148503474 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2354 0.3243402624207578 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1704 0.23478156634025968 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1607 0.22141665323286228 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1537 0.2117718705780394 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1533 0.21122074014062092 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1490 0.2052960879383726 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1480 0.20391826184482648 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1363 0.18779769655033682 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1221 0.16823256602198183 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1158 0.15955226163264125 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1004 0.13833373979203092 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 751 0.10347473962531398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.889130467730624E-5 0.0 14-15 0.0 0.0 0.0 1.3778260935461248E-4 0.0 16-17 0.0 0.0 0.0 4.1334782806383744E-4 0.0 18-19 0.0 0.0 0.0 5.511304374184499E-4 0.0 20-21 0.0 0.0 0.0 6.200217420957562E-4 0.0 22-23 0.0 0.0 0.0 8.955869608049811E-4 0.0 24-25 0.0 0.0 0.0 0.0012400434841915123 0.0 26-27 0.0 0.0 0.0 0.001791173921609962 0.0 28-29 0.0 0.0 0.0 0.0028245434917695557 0.0 30-31 0.0 0.0 0.0 0.004822391327411437 0.0 32-33 0.0 0.0 0.0 0.007715826123858299 0.0 34-35 0.0 0.0 0.0 0.010058130482886712 0.0 36-37 0.0 0.0 0.0 0.013709369630783941 0.0 38-39 0.0 0.0 0.0 0.016740587036585416 0.0 40-41 0.0 0.0 0.0 0.021356304449964933 0.0 42-43 0.0 0.0 0.0 0.029209913183177845 0.0 44-45 0.0 0.0 0.0 0.039061369752032636 0.0 46-47 0.0 0.0 0.0 0.06785793510714665 0.0 48-49 0.0 0.0 0.0 0.1294467614886584 0.0 50-51 0.0 0.0 0.0 0.16733697906117684 0.0 52-53 0.0 0.0 0.0 0.19840695747064196 0.0 54-55 0.0 0.0 0.0 0.2476642403149159 0.0 56-57 0.0 0.0 0.0 0.2848655448406613 0.0 58-59 0.0 0.0 0.0 0.32695813199849544 0.0 60-61 0.0 0.0 0.0 0.4057008932446564 0.0 62-63 0.0 0.0 0.0 0.44972243693345515 0.0 64-65 0.0 0.0 0.0 0.5093134154793251 0.0 66-67 0.0 0.0 0.0 0.6302176551879974 0.0 68-69 0.0 0.0 0.0 0.7562887427474678 0.0 70-71 0.0 0.0 0.0 0.8633458302160018 0.0 72-73 0.0 0.0 0.0 0.9915525482204687 0.0 74-75 0.0 0.0 0.0 1.0961295487206195 0.0 76-77 0.0 0.0 0.0 1.168189853413082 0.0 78-79 0.0 0.0 0.0 1.2701489843354952 0.0 80-81 0.0 0.0 0.0 1.411169485009941 0.0 82-83 0.0 0.0 0.0 1.5726507031735468 0.0 84-85 0.0 0.0 0.0 1.753352595342121 0.0 86-87 0.0 0.0 0.0 2.1195098797020036 0.0 88-89 0.0 0.0 0.0 2.5978222080765407 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 30 0.003947434 31.672985 76-77 TTGCGTA 95 1.19252945E-8 24.998096 60-61 GAGTACT 4420 0.0 24.499552 12-13 GTACTTT 4640 0.0 23.082832 14-15 AGTACTT 4465 0.0 22.072025 12-13 TATAGGG 175 4.043679E-6 21.715624 2 GGTGTGC 465 0.0 21.448502 8 GTACATG 3695 0.0 20.956728 1 AGAGTAC 7160 0.0 20.728487 10-11 ACTTTTT 5345 0.0 20.482538 16-17 GAACGAT 140 4.8541135E-4 20.35419 6 TACATGG 3720 0.0 20.048279 2 TACTTTT 5030 0.0 19.735079 14-15 GTAGGAC 195 1.0324657E-5 19.485697 3 ACATGGG 3485 0.0 19.352901 3 TATCTCG 150 3.7252903E-9 18.997246 88-89 TATACTG 355 7.8216544E-11 18.729677 5 CATGGGA 2215 0.0 17.5821 4 GTCTATA 165 0.0014618295 17.274982 1 GCGTAAG 125 4.5651977E-6 17.098696 62-63 >>END_MODULE