FastQCFastQC Report
Thu 26 May 2016
SRR936921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759203
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43870.577842816743348No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41710.549391928114088No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35980.4739180430003569No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.4212312122054312No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24050.31677957015449093No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22240.29293877921978706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.2621169832047555No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15200.20020995702071778No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15110.19902450332783198No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12050.15871907776971375No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11820.15568958499900554No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC9570.1260532426768598No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8770.11551587651787466No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8590.11314496913210301No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8560.11274981790114107No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA7750.1020807346651686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACTA753.0767074E-431.6680079
GTACATG37700.024.0691761
TAGGCCG1000.001651455923.7541355
GAGTACT30700.023.44077312-13
TACATGG37550.023.2796842
GTGTAAG3150.022.6229841
AGTACTT32650.022.33175712-13
ACATGGG38000.022.2538723
GTATAGA2405.9080776E-921.7746221
GTACTTT33800.021.14825614-15
AGCGACT1150.00372121720.653058
AGAGTAC56700.020.10667210-11
CATGGGA25300.019.341314
GTGTAGC4250.019.0033071
TACTTTT39200.018.65904414-15
TACCTGG5100.018.6306932
ACCGACT1559.617688E-418.3878758
ACTTTTT40900.017.65239516-17
GTATCAA93650.017.603171
CTTAGAC1901.862143E-417.5030483
Produced by FastQC (version 0.11.5)