##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936921_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759203 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.965241180553818 33.0 31.0 34.0 30.0 34.0 2 32.09984286152715 33.0 31.0 34.0 30.0 34.0 3 32.10307651576719 34.0 31.0 34.0 30.0 34.0 4 35.658199453901 37.0 35.0 37.0 35.0 37.0 5 35.66072578743761 37.0 35.0 37.0 35.0 37.0 6 35.76087554975415 37.0 35.0 37.0 35.0 37.0 7 35.713565410041845 37.0 35.0 37.0 35.0 37.0 8 35.67862350385865 37.0 35.0 37.0 33.0 37.0 9 37.31742498383173 39.0 37.0 39.0 34.0 39.0 10-11 37.20256505835725 39.0 37.0 39.0 34.0 39.0 12-13 37.13509100991435 39.0 37.0 39.0 34.0 39.0 14-15 38.309961894249625 40.0 38.0 41.0 33.5 41.0 16-17 38.24147230714315 40.0 38.0 41.0 33.0 41.0 18-19 38.22616085552876 40.0 38.0 41.0 33.5 41.0 20-21 38.12994811664338 40.0 38.0 41.0 33.5 41.0 22-23 37.9890259917308 40.0 38.0 41.0 33.0 41.0 24-25 38.10513393650973 40.0 38.0 41.0 33.0 41.0 26-27 38.05236478254169 40.0 38.0 41.0 33.0 41.0 28-29 37.96155837108125 40.0 38.0 41.0 33.0 41.0 30-31 37.7689484894027 40.0 37.5 41.0 33.0 41.0 32-33 37.53412987040357 40.0 37.0 41.0 33.0 41.0 34-35 37.32184014025235 40.0 37.0 41.0 32.0 41.0 36-37 37.12436265399373 40.0 36.0 41.0 31.5 41.0 38-39 36.954726864883305 39.0 36.0 41.0 31.0 41.0 40-41 36.78281763902409 39.0 36.0 41.0 31.0 41.0 42-43 36.538097847347814 39.0 35.5 41.0 30.0 41.0 44-45 36.22295486187489 39.0 35.0 40.5 30.0 41.0 46-47 35.82088848437111 39.0 35.0 40.0 28.5 41.0 48-49 35.52243668689402 39.0 35.0 40.0 27.0 41.0 50-51 34.83260669412529 38.0 34.0 39.5 26.5 40.5 52-53 35.037822558656906 38.5 35.0 40.0 27.0 40.5 54-55 35.43863630675854 39.0 35.0 40.5 27.0 41.0 56-57 35.303676355335796 39.0 35.0 41.0 26.5 41.0 58-59 35.05299702451123 39.0 35.0 40.5 26.0 41.0 60-61 34.75731128565087 38.0 34.5 40.0 26.0 41.0 62-63 34.44868105104959 38.0 34.0 40.0 25.0 41.0 64-65 34.10472561357108 37.5 34.0 40.0 23.5 41.0 66-67 33.715679469127494 37.0 34.0 40.0 22.5 41.0 68-69 33.29758970920821 36.0 33.0 39.0 22.0 41.0 70-71 32.85600162275439 36.0 33.0 39.0 20.5 40.5 72-73 32.444067001842726 35.0 32.0 38.5 19.5 40.0 74-75 32.001003684126644 35.0 32.0 37.5 18.0 40.0 76-77 31.56628991192079 35.0 31.5 37.0 16.0 39.0 78-79 31.118191050351488 35.0 31.0 36.5 11.5 39.0 80-81 30.61867181768249 35.0 31.0 36.0 7.0 38.5 82-83 30.16374276708601 34.0 30.0 35.5 4.5 37.0 84-85 29.7519148370067 34.0 30.0 35.0 2.0 37.0 86-87 29.428382790900457 34.0 30.0 35.0 2.0 36.5 88-89 29.046712144182784 34.0 29.0 35.0 2.0 36.0 90-91 28.617822901121308 34.0 29.0 35.0 2.0 36.0 92-93 28.213382323304835 34.0 29.0 35.0 2.0 35.0 94-95 27.789589213952 34.0 27.5 35.0 2.0 35.0 96-97 27.274792776108633 34.0 26.5 35.0 2.0 35.0 98-99 26.628978020371363 34.0 25.5 35.0 2.0 35.0 100-101 24.15895880284983 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3236831623712497 1101 2 0.24677231143206768 1101 3 0.1659112440867645 1101 4 0.14301063993212892 1101 5 0.12895109375296698 1101 6 0.21144033785341776 1101 7 0.16251431335276578 1101 8 0.06375847253398348 1101 9 0.25393857893234184 1101 10-11 0.27939320948497226 1101 12-13 0.4102204706270456 1101 14-15 0.40683961270840996 1101 16-17 0.39975690855802526 1101 18-19 0.3191286310672794 1101 20-21 0.4233017253959801 1101 22-23 0.4591430084096402 1101 24-25 0.2382446756298151 1101 26-27 0.2188681757176525 1101 28-29 0.11432282163629992 1101 30-31 0.09185449390692213 1101 32-33 0.3796193716025158 1101 34-35 0.2874900288007325 1101 36-37 0.20267881613626315 1101 38-39 0.36274677132050925 1101 40-41 0.08553216864179802 1101 42-43 0.39275002832263084 1101 44-45 0.1494488279945827 1101 46-47 0.34540755274417734 1101 48-49 0.36776066823900777 1101 50-51 0.26398625932439046 1101 52-53 0.26437261272297263 1101 54-55 0.2835181031144529 1101 56-57 -0.04543555551301637 1101 58-59 -0.32622047391366493 1101 60-61 0.0396970196764741 1101 62-63 0.049209759996990954 1101 64-65 0.14804067507430574 1101 66-67 0.1802649808791017 1101 68-69 0.36333119032315153 1101 70-71 0.17210618231256092 1101 72-73 0.05737314850158981 1101 74-75 0.14603695252662519 1101 76-77 0.2846966030105804 1101 78-79 0.0808888750669361 1101 80-81 0.03868503601863438 1101 82-83 -0.009128623255183044 1101 84-85 0.00551325625317034 1101 86-87 0.011466590057029435 1101 88-89 0.03273428344753171 1101 90-91 0.24640253077897256 1101 92-93 0.3600762914281326 1101 94-95 0.4791756096641322 1101 96-97 0.16566585070204454 1101 98-99 0.056772204216979816 1101 100-101 0.3130395771670429 1102 1 0.15729794799841912 1102 2 0.20633699855093823 1102 3 0.11717308853746289 1102 4 0.1443240839700337 1102 5 0.1263242056771645 1102 6 0.045055123480587156 1102 7 0.049370491230611435 1102 8 0.042649550496271615 1102 9 0.193895422913954 1102 10-11 0.19181463738627258 1102 12-13 0.0849554363836873 1102 14-15 0.06632924588225819 1102 16-17 0.19950360149356783 1102 18-19 0.23914927090216764 1102 20-21 0.29111296472424897 1102 22-23 0.27472139287347375 1102 24-25 -0.13395252861072748 1102 26-27 -0.1362542560301705 1102 28-29 0.007136406400356066 1102 30-31 7.577681352657351E-4 1102 32-33 0.09145913142990025 1102 34-35 0.046754500139513766 1102 36-37 -0.1352515713297251 1102 38-39 0.09780634538936539 1102 40-41 0.09184139089528998 1102 42-43 0.07527184087539496 1102 44-45 -0.24296603268653172 1102 46-47 -0.12227234218030958 1102 48-49 -0.18709907470123 1102 50-51 -0.2715470927724368 1102 52-53 -0.12292919795344659 1102 54-55 0.0021127179939028906 1102 56-57 0.12071511528161238 1102 58-59 0.007933761943348827 1102 60-61 -0.22428177758406065 1102 62-63 -0.06247989194033465 1102 64-65 0.01592227747602948 1102 66-67 -0.14009809257994732 1102 68-69 -0.07073860984571922 1102 70-71 -0.07953562273481651 1102 72-73 -0.0472098330163746 1102 74-75 0.16318208809281387 1102 76-77 0.1946084146251792 1102 78-79 0.15469973912547985 1102 80-81 0.3498305468545482 1102 82-83 0.34864415092625833 1102 84-85 0.21306086859954476 1102 86-87 0.39836967665052114 1102 88-89 0.3834003872096119 1102 90-91 0.2125344380873493 1102 92-93 0.16929854492282814 1102 94-95 0.0690527088291546 1102 96-97 -0.18380408081122823 1102 98-99 -0.06366592318709152 1102 100-101 0.005764035371374376 1103 1 0.007076859941836489 1103 2 0.18179511061060083 1103 3 0.10323960904949558 1103 4 0.1906608314672127 1103 5 0.1511970097779738 1103 6 0.0779547657305173 1103 7 0.0936899229033159 1103 8 0.1529528160660334 1103 9 0.1274374879792859 1103 10-11 -0.041752776705450856 1103 12-13 0.25296751058102984 1103 14-15 0.22451671625094605 1103 16-17 0.2799114556613702 1103 18-19 0.20117929305869353 1103 20-21 0.22417668623708664 1103 22-23 0.3018639424340037 1103 24-25 0.040844917819562454 1103 26-27 0.30239844852003017 1103 28-29 0.1398809847314979 1103 30-31 0.4266479273038186 1103 32-33 0.37461252910096476 1103 34-35 0.18345469327999808 1103 36-37 0.5291623199367379 1103 38-39 0.5615970112202575 1103 40-41 0.5726848452781823 1103 42-43 0.3450348009628783 1103 44-45 0.40090984405568264 1103 46-47 0.6277491355512694 1103 48-49 0.6193664119099225 1103 50-51 0.42146134877153685 1103 52-53 0.37030452113054935 1103 54-55 0.45552778337636113 1103 56-57 0.5237662259189761 1103 58-59 0.7105233930248716 1103 60-61 0.7159088386094155 1103 62-63 0.6173645562516654 1103 64-65 0.6314698217493202 1103 66-67 0.6057206438506384 1103 68-69 0.6512324201414401 1103 70-71 0.6694777273988777 1103 72-73 0.38164955625239827 1103 74-75 0.4029939537705509 1103 76-77 0.4258843403684409 1103 78-79 0.17357362718884417 1103 80-81 0.40392897532097294 1103 82-83 0.25770054137338505 1103 84-85 0.1593729034475615 1103 86-87 0.33132468371960755 1103 88-89 0.297970986369382 1103 90-91 0.06278532981094642 1103 92-93 0.1530104696775858 1103 94-95 0.2804407854532158 1103 96-97 -0.27962711278812336 1103 98-99 0.04715652014086302 1103 100-101 0.274433487249059 1104 1 0.16185161156910866 1104 2 0.2271477570648699 1104 3 0.18202086975521325 1104 4 0.09594624599273516 1104 5 0.15580046986704588 1104 6 0.1801847674434356 1104 7 0.06236391228324578 1104 8 0.14162723367480368 1104 9 0.14362775005533734 1104 10-11 0.3234161595741014 1104 12-13 0.2242049848873009 1104 14-15 0.2506397463982495 1104 16-17 0.23163079447602541 1104 18-19 0.10503549381238031 1104 20-21 0.38125905605551225 1104 22-23 0.26047990680536515 1104 24-25 0.2959429822251778 1104 26-27 0.2738286258067788 1104 28-29 0.2597550854509194 1104 30-31 0.1204139858855271 1104 32-33 0.23623144825135967 1104 34-35 0.37563898156366093 1104 36-37 0.4688441459045336 1104 38-39 0.3424899754203281 1104 40-41 0.40076166953306114 1104 42-43 0.5357368821550708 1104 44-45 0.5543201515240028 1104 46-47 0.5220146160240233 1104 48-49 0.39292221012848927 1104 50-51 0.32775631280031803 1104 52-53 0.39575630256255323 1104 54-55 0.35626487227666814 1104 56-57 0.5321686111536437 1104 58-59 0.6425490225212656 1104 60-61 0.792189778143495 1104 62-63 0.8398904640968183 1104 64-65 0.7890082552882021 1104 66-67 0.4959345441589633 1104 68-69 0.3560061011965914 1104 70-71 0.5304617972858239 1104 72-73 0.5228366066121097 1104 74-75 0.49116841420220325 1104 76-77 0.4300815183403479 1104 78-79 0.5697313438726397 1104 80-81 0.45771077131275106 1104 82-83 0.31567362719044567 1104 84-85 0.4961716787130648 1104 86-87 0.523529312873432 1104 88-89 0.3875944661569797 1104 90-91 0.25273051651872436 1104 92-93 0.08534924151725676 1104 94-95 0.36524401429871034 1104 96-97 0.2270350574756641 1104 98-99 0.6110809163381923 1104 100-101 0.5523165379513593 1105 1 0.19802562217702402 1105 2 0.22030488680783122 1105 3 0.294867666980835 1105 4 0.22479544019918052 1105 5 0.18061177137663265 1105 6 0.15346225622352705 1105 7 0.04610651734240179 1105 8 0.05812942665725984 1105 9 0.154991734143465 1105 10-11 0.19233063325830813 1105 12-13 0.19685454119085222 1105 14-15 0.13447670070262063 1105 16-17 0.2441792440884356 1105 18-19 0.1710978153138072 1105 20-21 0.014983173762573188 1105 22-23 0.04819920505097741 1105 24-25 0.007466340038320141 1105 26-27 -0.04965094885663035 1105 28-29 -0.11232263792729213 1105 30-31 -0.11902527360039983 1105 32-33 0.321797288724639 1105 34-35 -0.01487700680843318 1105 36-37 -0.007392594201540703 1105 38-39 0.13612203886381735 1105 40-41 0.30150371359670913 1105 42-43 0.14599927505592092 1105 44-45 -0.050391573282205115 1105 46-47 0.035874392645816044 1105 48-49 0.06167093513869304 1105 50-51 0.3523279162274875 1105 52-53 0.39258667791954593 1105 54-55 0.3833016684822965 1105 56-57 0.5357557949561738 1105 58-59 0.3163192948590847 1105 60-61 0.29149809617695155 1105 62-63 0.275869401265318 1105 64-65 0.2322565468018709 1105 66-67 0.33799008271465425 1105 68-69 0.4865969314699541 1105 70-71 0.5458993737852111 1105 72-73 0.3859027020833494 1105 74-75 0.3647684754017142 1105 76-77 0.5187258216859227 1105 78-79 0.462113279817153 1105 80-81 0.32934671072523614 1105 82-83 0.3591080672149296 1105 84-85 0.4444670173503553 1105 86-87 0.15225164559476667 1105 88-89 0.14798627052254432 1105 90-91 0.017092001626842546 1105 92-93 0.28953758301052446 1105 94-95 0.008021197081248488 1105 96-97 0.02545024515416472 1105 98-99 -0.3085239643227631 1105 100-101 0.012759979403824673 1106 1 0.20928252694043792 1106 2 0.18989063271254025 1106 3 0.25894228599010205 1106 4 0.3354270318303847 1106 5 0.21160789646462774 1106 6 0.3186184893778048 1106 7 0.3378442663419676 1106 8 0.40500698324302675 1106 9 0.565567516894717 1106 10-11 0.4458416777088061 1106 12-13 0.4098510341100976 1106 14-15 0.4579961799042991 1106 16-17 0.35586438687671773 1106 18-19 0.20761537291428311 1106 20-21 0.3074383522558648 1106 22-23 0.44774242839431366 1106 24-25 0.46195118602484087 1106 26-27 0.39762234342335745 1106 28-29 0.38987166576180243 1106 30-31 0.3336426128285268 1106 32-33 0.17383399574704583 1106 34-35 0.04454280280718592 1106 36-37 0.0870544222430496 1106 38-39 0.14351275175884126 1106 40-41 0.2681515659061162 1106 42-43 -0.013437340642859397 1106 44-45 0.2114472024739129 1106 46-47 0.1319806134333561 1106 48-49 0.12034556936855978 1106 50-51 0.01389620917337453 1106 52-53 0.23441131119812297 1106 54-55 0.10296985932330216 1106 56-57 0.25421902134362995 1106 58-59 0.29304286967152393 1106 60-61 0.24910633686543093 1106 62-63 0.44374948827643834 1106 64-65 0.3527847017476162 1106 66-67 0.3036256063351246 1106 68-69 0.4593361961879623 1106 70-71 0.4547493620526595 1106 72-73 0.6484842750058917 1106 74-75 0.6075220411452058 1106 76-77 0.6156897869068452 1106 78-79 0.5398144613165066 1106 80-81 0.48207119877389104 1106 82-83 0.6275178629417439 1106 84-85 0.8833856251724761 1106 86-87 0.708846130662721 1106 88-89 0.9776986328236461 1106 90-91 0.9324369068468741 1106 92-93 0.5297631016208832 1106 94-95 0.6673111559221994 1106 96-97 0.7475283649022622 1106 98-99 0.666561810120573 1106 100-101 0.18668074347812436 1107 1 0.07935582564411803 1107 2 0.19006258007371457 1107 3 0.29390982887868944 1107 4 0.16162918952412753 1107 5 0.35583734302338854 1107 6 0.22077552808397627 1107 7 0.035508402380166615 1107 8 0.2778379901012684 1107 9 0.16485483602246376 1107 10-11 0.11863976892934147 1107 12-13 0.33948034835126606 1107 14-15 0.3409434231787003 1107 16-17 0.5689227245223876 1107 18-19 0.3077878115536379 1107 20-21 0.44628420881574016 1107 22-23 0.4413058655074309 1107 24-25 0.5212722068353841 1107 26-27 0.35741941278528344 1107 28-29 0.4206748073219657 1107 30-31 0.5116236887932359 1107 32-33 0.4140035194692331 1107 34-35 0.21500568510347762 1107 36-37 0.34689144889652823 1107 38-39 0.26665572025807904 1107 40-41 0.3329282308478554 1107 42-43 0.28566181894394305 1107 44-45 0.3621418698130796 1107 46-47 0.30401722348294413 1107 48-49 0.1365471047766107 1107 50-51 0.3318334303730168 1107 52-53 0.43360659754780073 1107 54-55 0.3991516453487307 1107 56-57 0.33593823294041414 1107 58-59 0.2717426856059717 1107 60-61 0.05878599074357993 1107 62-63 0.3894861762754971 1107 64-65 0.23869680880695654 1107 66-67 -0.054235335739896584 1107 68-69 -0.12037532526780126 1107 70-71 -0.08510394228434137 1107 72-73 -0.23585492309712208 1107 74-75 0.010534722672495889 1107 76-77 -0.29799673176500363 1107 78-79 -0.5491068883228927 1107 80-81 -0.3270099145279559 1107 82-83 -0.0063218690168831415 1107 84-85 -0.10601832339397177 1107 86-87 -0.10643736959601924 1107 88-89 -0.043319066291395814 1107 90-91 -0.09667951297980437 1107 92-93 -0.10598688364077091 1107 94-95 -0.08080397348047086 1107 96-97 -0.1411447254994691 1107 98-99 -0.2725098706403166 1107 100-101 -0.373166763776414 1108 1 0.16615755400178855 1108 2 0.3444614757278188 1108 3 0.22715782425846243 1108 4 0.236072174497842 1108 5 0.44056137980205534 1108 6 0.20457833754556987 1108 7 0.1643340840566907 1108 8 0.2861751677509403 1108 9 0.22089968598848486 1108 10-11 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86-87 -2.8252858674384633 2316 88-89 -2.90509863813152 2316 90-91 -2.6159829337736724 2316 92-93 -3.92094339980828 2316 94-95 -3.464257691354309 2316 96-97 -3.4140529054872992 2316 98-99 -3.0200929384376387 2316 100-101 -3.131376200840066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1511.0 3 487.0 4 514.0 5 524.0 6 558.0 7 697.0 8 854.0 9 1017.0 10 1314.0 11 1783.0 12 2471.0 13 3104.0 14 3506.0 15 4528.0 16 5929.0 17 8200.0 18 7338.0 19 4444.0 20 4140.0 21 4208.0 22 4668.0 23 5099.0 24 5659.0 25 6517.0 26 7566.0 27 8832.0 28 10565.0 29 12870.0 30 16033.0 31 19743.0 32 25760.0 33 34434.0 34 47684.0 35 70148.0 36 106495.0 37 155265.0 38 139642.0 39 25081.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.48761528326746 18.95638998682477 14.96205533596838 21.593939393939394 2 14.920565918431869 20.77049741806302 40.851644008852354 23.457292654652758 3 19.977947772843617 25.3275229974167 30.377535709722082 24.3169935200176 4 12.245736864086892 16.825931776650283 39.175923278704936 31.75240808055789 5 13.030063802054158 37.71490854072405 34.64763895496954 14.607388702252248 6 29.66901188510494 36.684373307469606 18.357934187170308 15.28868062025515 7 26.71976561285546 33.596852003593796 21.720990770479233 17.96239161307151 8 25.398220641976348 33.37291269390482 20.750830573540515 20.47803609057831 9 25.588516645540764 17.400934458759696 21.854370953248075 35.156177942451464 10-11 24.216253960725414 26.525892110169103 27.895759635843824 21.36209429326166 12-13 26.187153782500307 24.38596629930058 28.152193391627005 21.27468652657211 14-15 22.767646001003882 25.487416557210103 27.9749898887166 23.769947553069418 16-17 20.946764894973704 28.8119460165892 28.29294063089554 21.948348457541552 18-19 20.69110433226337 27.647005739331714 30.80558137328974 20.85630855511517 20-21 22.15798612025947 26.021220089264542 30.45111791456108 21.369675875914908 22-23 21.410172054611188 26.0850149871863 30.82842158023101 21.676391377971505 24-25 21.79794609639108 26.156876717705813 30.695275284069766 21.34990190183334 26-27 21.31722345670394 25.946749420115566 31.514035113138384 21.221992010042108 28-29 20.79113369661573 27.04958844240367 30.990240901804427 21.169036959176175 30-31 21.14329105654219 26.167112089915346 30.81685662464453 21.872740228897936 32-33 21.117939200514225 26.27257226516579 31.014073357433247 21.595415176886736 34-35 21.911728483686183 26.21334478393789 30.50784836203229 21.367078370343638 36-37 21.549374804894082 25.910461365405563 31.302629204573744 21.237534625126614 38-39 21.17209912513402 26.576128619260754 30.964157101798996 21.287615153806232 40-41 21.237534625126614 26.78183568821514 30.699760143202802 21.280869543455438 42-43 20.970741685688807 26.797246586222656 30.004425693786775 22.227586034301762 44-45 21.373861799808484 26.387935769484578 30.097154515985842 22.141047914721096 46-47 21.9520339092443 26.906901052814597 29.654387561692985 21.48667747624812 48-49 21.72640824130121 26.875443311747027 29.53185620926224 21.866292237689517 50-51 21.04922079316229 27.24217483764192 30.065996971818414 21.642607397377375 52-53 21.6258099017778 27.65099177156917 28.82967135012388 21.89352697652915 54-55 21.234257955486946 26.959877132692213 29.32785207450543 22.47801283731541 56-57 21.794251204388814 27.05536401684662 28.93305804446113 22.21732673430343 58-59 22.384455144353275 26.993867858979854 28.81078877471116 21.81088822195571 60-61 21.723106428323984 27.03596083705332 29.899550440141887 21.341382294480805 62-63 21.352573327230278 27.288168523378765 29.60546377188621 21.75379437750475 64-65 21.076794905146553 27.824314489971318 29.223884430599416 21.875006174282714 66-67 21.383739763499204 27.304031895266757 28.679393420843763 22.632834920390273 68-69 21.17797548513649 27.323882988918974 29.131572585877443 22.366568940067094 70-71 21.84855349756693 27.094327491813463 28.87372607195721 22.183392938662394 72-73 21.60819114066798 26.692742640936736 29.51997460029721 22.179091618098077 74-75 21.060924438940525 28.09407750426517 28.77940333704409 22.065594719750216 76-77 21.31906993658419 27.308194513801144 28.627369857923814 22.745365691690857 78-79 21.49205811772812 27.336964648364294 28.81658609398251 22.354391139925077 80-81 21.815861276917065 27.492519788544424 28.460779707854993 22.230839226683514 82-83 21.52826183489454 27.262389629487128 29.13979188874731 22.069556646871018 84-85 21.440094222483367 27.52667957307413 28.701035301154263 22.332190903288236 86-87 21.296478042932705 27.726964345701134 28.67269577589103 22.30386183547514 88-89 21.259766281087106 27.462696791140235 28.178923670527734 23.098613257244928 90-91 21.409768938286046 27.025709535215235 28.585356342010332 22.97916518448838 92-93 21.740178784950125 27.301037674754745 28.756522936426528 22.202260603868602 94-95 21.343693622783853 27.171586969852452 28.83839305297672 22.646326354386975 96-97 20.611894837244353 27.37724161190538 29.389027365714078 22.621836185136196 98-99 20.934620444674 27.765740114274724 28.357997550597165 22.941641890454115 100-101 20.85204231221566 27.261927900564313 28.6578620537435 23.228167733476525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 155.0 1 132.5 2 137.5 3 174.0 4 154.0 5 147.5 6 273.0 7 618.5 8 1050.0 9 1253.0 10 1102.5 11 953.5 12 1014.0 13 1095.0 14 1170.0 15 1238.5 16 1310.0 17 1399.0 18 1500.0 19 1625.5 20 1776.5 21 2038.0 22 2342.5 23 2774.0 24 3343.5 25 4030.0 26 5073.5 27 6338.0 28 7316.5 29 8446.5 30 9997.0 31 11624.5 32 13334.0 33 14983.5 34 16767.5 35 18153.0 36 19867.0 37 21707.5 38 22465.5 39 22728.5 40 23033.5 41 23962.0 42 24700.5 43 25094.0 44 25836.0 45 26704.5 46 27347.5 47 30931.5 48 38742.5 49 37835.0 50 29618.5 51 25247.0 52 23177.0 53 22227.0 54 21040.0 55 19281.0 56 17543.5 57 15607.5 58 13980.5 59 12463.5 60 10448.5 61 8424.5 62 6572.5 63 5016.5 64 3652.5 65 2612.0 66 1860.5 67 1392.0 68 1008.5 69 648.0 70 489.0 71 345.0 72 218.5 73 167.0 74 134.5 75 92.5 76 59.0 77 43.5 78 27.5 79 15.5 80 12.0 81 10.0 82 9.5 83 6.5 84 4.0 85 4.0 86 4.5 87 4.0 88 4.0 89 3.0 90 2.5 91 1.5 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026738566628424807 2 0.01093251738994709 3 0.012644839390782175 4 0.025816547089513607 5 0.059272684644291446 6 0.04662784525350927 7 0.015410898007515777 8 0.05136966002505258 9 0.03437815709368904 10-11 0.02476281047361509 12-13 0.03780280109535921 14-15 0.01975756154809715 16-17 0.020416146933033723 18-19 0.01876968347069229 20-21 0.01462059554559189 22-23 0.007178580695808631 24-25 0.0019098976163160577 26-27 0.0 28-29 0.0018440390778224004 30-31 0.0 32-33 3.292926924682858E-4 34-35 0.0 36-37 0.0 38-39 1.317170769873143E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 3.292926924682858E-4 50-51 1.975756154809715E-4 52-53 7.90302461923886E-4 54-55 0.0013171707698731432 56-57 6.585853849365715E-5 58-59 8.561610004175432E-4 60-61 0.002107473231797029 62-63 0.002700200078239944 64-65 7.244439234302287E-4 66-67 0.012315546698313891 68-69 0.014818171161072861 70-71 0.024038366550184865 72-73 0.01830867370123669 74-75 0.019494127394122523 76-77 0.032138966784904695 78-79 0.02074543962550201 80-81 0.048406025792838016 82-83 0.037144215710422646 84-85 0.019559985932616177 86-87 0.014225444314629947 88-89 0.011327668620909033 90-91 0.02436765924265315 92-93 0.03246825947737298 94-95 0.01139352715940269 96-97 0.019889278625084463 98-99 0.031941391169423725 100-101 0.042215323174434244 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759203.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.6491073015769 #Duplication Level Percentage of deduplicated Percentage of total 1 86.68180892135048 61.23992419579276 2 8.526485519837017 12.047771807926145 3 2.120350288826206 4.494025652166367 4 0.8583333044714752 2.4256192691248932 5 0.4654358464452195 1.6441313528754293 6 0.296790474220715 1.2580789235583114 7 0.19027051581066512 0.9409709461483846 8 0.14276030847125085 0.8068710681273297 9 0.11666734536327661 0.7418199421044234 >10 0.5590459567066883 6.998898378583926 >50 0.027847813849521147 1.3437798002633323 >100 0.010092105944749442 1.3817642487404425 >500 0.0020557993513930675 1.0597266925907205 >1k 0.0020557993513930675 3.6166177219975606 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4387 0.577842816743348 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4171 0.549391928114088 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3598 0.4739180430003569 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3198 0.4212312122054312 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2405 0.31677957015449093 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2224 0.29293877921978706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.2621169832047555 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1520 0.20020995702071778 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1511 0.19902450332783198 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1205 0.15871907776971375 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1182 0.15568958499900554 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 957 0.1260532426768598 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 877 0.11551587651787466 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 859 0.11314496913210301 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 856 0.11274981790114107 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 775 0.1020807346651686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.585853849365717E-5 0.0 18-19 0.0 0.0 0.0 1.3171707698731434E-4 0.0 20-21 0.0 0.0 0.0 1.3171707698731434E-4 0.0 22-23 0.0 0.0 0.0 2.634341539746287E-4 0.0 24-25 0.0 0.0 0.0 6.585853849365716E-4 0.0 26-27 0.0 0.0 0.0 0.0010537366158985147 0.0 28-29 0.0 0.0 0.0 0.002436765924265315 0.0 30-31 0.0 0.0 0.0 0.005532117233467202 0.0 32-33 0.0 0.0 0.0 0.00928605392760566 0.0 34-35 0.0 0.0 0.0 0.012710697929275833 0.0 36-37 0.0 0.0 0.0 0.015674332161490404 0.0 38-39 0.0 0.0 0.0 0.01830867370123669 0.0 40-41 0.0 0.0 0.0 0.022128468933868807 0.0 42-43 0.0 0.0 0.0 0.028450888629259895 0.0 44-45 0.0 0.0 0.0 0.03786865963385287 0.0 46-47 0.0 0.0 0.0 0.0653316701857079 0.0 48-49 0.0 0.0 0.0 0.11953324736598775 0.0 50-51 0.0 0.0 0.0 0.15292352638227194 0.0 52-53 0.0 0.0 0.0 0.1861820883215688 0.0 54-55 0.0 0.0 0.0 0.23188791403616688 0.0 56-57 0.0 0.0 0.0 0.26863697851562757 0.0 58-59 0.0 0.0 0.0 0.3110498773055428 0.0 60-61 0.0 0.0 0.0 0.37987205003141455 0.0 62-63 0.0 0.0 0.0 0.4291342368246701 0.0 64-65 0.0 0.0 0.0 0.4874190433915567 0.0 66-67 0.0 6.585853849365717E-5 0.0 0.6000371442157104 0.0 68-69 0.0 1.3171707698731434E-4 0.0 0.718450796427306 0.0 70-71 0.0 1.3171707698731434E-4 0.0 0.8215852677083731 0.0 72-73 0.0 1.3171707698731434E-4 0.0 0.9411185150743608 0.0 74-75 0.0 1.3171707698731434E-4 0.0 1.0399063228148466 0.0 76-77 0.0 1.3171707698731434E-4 0.0 1.1101115248490852 0.0 78-79 0.0 1.3171707698731434E-4 0.0 1.2154851864389367 0.0 80-81 0.0 1.3171707698731434E-4 0.0 1.3610984150484127 0.0 82-83 0.0 1.3171707698731434E-4 0.0 1.5198174928181265 0.0 84-85 0.0 1.3171707698731434E-4 0.0 1.7040238249848856 0.0 86-87 0.0 1.3171707698731434E-4 0.0 2.0593964986966595 0.0 88-89 0.0 1.3171707698731434E-4 0.0 2.5358171661597755 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGACTA 75 3.0767074E-4 31.668007 9 GTACATG 3770 0.0 24.069176 1 TAGGCCG 100 0.0016514559 23.754135 5 GAGTACT 3070 0.0 23.440773 12-13 TACATGG 3755 0.0 23.279684 2 GTGTAAG 315 0.0 22.622984 1 AGTACTT 3265 0.0 22.331757 12-13 ACATGGG 3800 0.0 22.253872 3 GTATAGA 240 5.9080776E-9 21.774622 1 GTACTTT 3380 0.0 21.148256 14-15 AGCGACT 115 0.003721217 20.65305 8 AGAGTAC 5670 0.0 20.106672 10-11 CATGGGA 2530 0.0 19.34131 4 GTGTAGC 425 0.0 19.003307 1 TACTTTT 3920 0.0 18.659044 14-15 TACCTGG 510 0.0 18.630693 2 ACCGACT 155 9.617688E-4 18.387875 8 ACTTTTT 4090 0.0 17.652395 16-17 GTATCAA 9365 0.0 17.60317 1 CTTAGAC 190 1.862143E-4 17.503048 3 >>END_MODULE