FastQCFastQC Report
Thu 26 May 2016
SRR936921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences759203
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71430.9408550809203862No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.6970467714168674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43980.5792917045902084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.37012498633435326No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24560.323497141080844No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17680.23287579211357176No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17240.2270802407261299No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15990.2106156061027156No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15830.20850813287091857No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15240.20073682532866705No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15170.19981480578975583No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA13810.1819012833194811No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12710.1674124048508765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12330.16240715592535857No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10410.13711747714379421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTGCG300.00394970531.66935776-77
GTCGTAA300.00395097931.66727338-39
TAAGGTG3150.027.1442685
GGGCGTA1151.2807404E-424.7838979
CCTACGT1000.001653897323.7481082
GTAAGGT2602.3646862E-1123.7481084
GAGTACT46400.023.5433812-13
TGTATCG1050.002194875922.6202249
GTACATG37100.022.2817211
TACATGG37050.021.664942
GTACTTT50500.021.49227714-15
AGTACTT48850.021.19585412-13
ACATGGG37100.020.995633
ACTTTTT55450.019.61649916-17
CATGGGA23200.019.448884
AGAGTAC72150.019.35651610-11
TACTTTT53350.019.09767314-15
GGTAAGG3357.3123374E-1018.4313663
CGCGGGG800.001191890917.8110888-89
AAGGTGT5100.017.6969996
Produced by FastQC (version 0.11.5)