##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936921_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 759203 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44126669678597 33.0 31.0 34.0 31.0 34.0 2 32.56360288355025 34.0 31.0 34.0 31.0 34.0 3 32.66428610002858 34.0 31.0 34.0 31.0 34.0 4 36.241658686807085 37.0 35.0 37.0 35.0 37.0 5 36.18616891661387 37.0 35.0 37.0 35.0 37.0 6 36.25365416100832 37.0 37.0 37.0 35.0 37.0 7 36.217827116067774 37.0 36.0 37.0 35.0 37.0 8 36.1927784795371 37.0 36.0 37.0 35.0 37.0 9 37.91655328021623 39.0 38.0 39.0 35.0 39.0 10-11 37.83958243052254 39.0 38.0 39.0 35.0 39.0 12-13 37.75748185926557 39.0 37.0 39.0 35.0 39.0 14-15 38.98592471315314 40.0 38.0 41.0 36.0 41.0 16-17 38.928380156558916 40.0 38.0 41.0 36.0 41.0 18-19 39.11044345188309 40.0 38.5 41.0 36.0 41.0 20-21 39.14473599287674 40.0 39.0 41.0 36.0 41.0 22-23 39.06204598770026 40.0 38.5 41.0 35.5 41.0 24-25 38.94332016601621 40.0 38.0 41.0 35.0 41.0 26-27 38.765005538703086 40.0 38.0 41.0 35.0 41.0 28-29 38.69425436938474 40.0 38.0 41.0 35.0 41.0 30-31 38.67419385855957 40.0 38.0 41.0 35.0 41.0 32-33 38.48094383188686 40.0 38.0 41.0 35.0 41.0 34-35 38.28987240566752 40.0 38.0 41.0 34.0 41.0 36-37 38.123748193829584 40.0 38.0 41.0 34.0 41.0 38-39 37.956691425086575 40.0 37.0 41.0 34.0 41.0 40-41 37.797812969653705 40.0 37.0 41.0 33.0 41.0 42-43 37.52354113458456 40.0 37.0 41.0 33.0 41.0 44-45 37.1786913381533 40.0 37.0 41.0 32.5 41.0 46-47 36.71823939051875 40.0 36.5 41.0 31.0 41.0 48-49 36.253777975060686 39.5 36.0 41.0 30.0 41.0 50-51 35.925159674026574 39.0 36.0 41.0 29.0 41.0 52-53 35.701946646680796 39.0 35.0 40.0 28.5 41.0 54-55 35.41739297658202 39.0 35.0 40.0 28.0 41.0 56-57 35.112028666904635 38.5 35.0 40.0 26.5 41.0 58-59 34.782844641024866 38.0 34.5 40.0 26.0 41.0 60-61 34.41332226031773 38.0 34.0 40.0 26.0 41.0 62-63 34.08968945064759 37.5 34.0 40.0 25.0 41.0 64-65 33.71421938532909 37.0 33.5 39.5 24.5 41.0 66-67 33.30958650058021 36.0 33.0 39.0 23.0 40.0 68-69 32.9806099290967 36.0 33.0 39.0 22.0 40.0 70-71 32.4867788983974 35.0 32.0 38.5 21.0 40.0 72-73 31.962664794527946 35.0 31.5 37.5 19.0 39.5 74-75 31.479312515888374 35.0 31.0 37.0 18.0 39.0 76-77 30.109709787764274 33.5 29.5 35.5 16.5 38.0 78-79 30.811619553663512 34.0 31.0 36.0 16.0 38.5 80-81 30.501001708370488 34.0 31.0 36.0 9.0 37.5 82-83 30.182047489274936 34.0 30.5 35.0 7.5 37.0 84-85 29.84884148244936 34.0 30.0 35.0 6.5 36.5 86-87 29.55753006771575 34.0 30.0 35.0 2.0 36.0 88-89 29.16571720607005 34.0 29.5 35.0 2.0 36.0 90-91 28.715294196677306 34.0 29.0 35.0 2.0 35.0 92-93 28.254272572684776 34.0 29.0 35.0 2.0 35.0 94-95 27.675281183030098 33.0 27.5 35.0 2.0 35.0 96-97 27.006182141008402 33.0 26.0 35.0 2.0 35.0 98-99 26.2411509174753 33.0 25.0 34.0 2.0 35.0 100-101 22.749603202305575 28.5 10.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05873569787629407 1101 2 0.03281103845352362 1101 3 0.10263850413126363 1101 4 0.05588932648147704 1101 5 0.17345796617082243 1101 6 0.12872259014469023 1101 7 0.17930194623917828 1101 8 0.1777270900585819 1101 9 0.1592320047599216 1101 10-11 0.19309566033164316 1101 12-13 0.21883170954453846 1101 14-15 0.2514547267439724 1101 16-17 0.20683014524338006 1101 18-19 0.12682148657129488 1101 20-21 0.19195761720072113 1101 22-23 0.2510612239209635 1101 24-25 0.1866971086258431 1101 26-27 0.09889244320055468 1101 28-29 0.16533894699422547 1101 30-31 0.3599618993744613 1101 32-33 0.46479765204987444 1101 34-35 0.4540006236654861 1101 36-37 0.4056016171872514 1101 38-39 0.30946798878830606 1101 40-41 0.32026522826674153 1101 42-43 0.39305961515152177 1101 44-45 0.5439425314545971 1101 46-47 0.7001244766489023 1101 48-49 0.9219569885707699 1101 50-51 0.8403874400485662 1101 52-53 0.8922871175835994 1101 54-55 0.9400051075758995 1101 56-57 0.9034074466660869 1101 58-59 0.9975763023045872 1101 60-61 1.0517209763393325 1101 62-63 0.9482258583232692 1101 64-65 0.7620885610996808 1101 66-67 0.7037017382443409 1101 68-69 0.5313944699373749 1101 70-71 0.526543871871219 1101 72-73 0.7669427967602758 1101 74-75 0.7416931593765597 1101 76-77 0.666599135626285 1101 78-79 0.8299201939701994 1101 80-81 1.2311881197016383 1101 82-83 1.09042672184178 1101 84-85 0.9711487663502929 1101 86-87 0.5334274886820012 1101 88-89 0.5112912775171985 1101 90-91 0.8750138163261489 1101 92-93 0.8724348229389669 1101 94-95 0.8804676140309091 1101 96-97 0.8437276070065067 1101 98-99 0.8357720154534043 1101 100-101 1.470952877962663 1102 1 0.18956410579448857 1102 2 0.124845938537959 1102 3 0.12280925185619651 1102 4 0.09402611229628377 1102 5 0.05914142615768725 1102 6 0.04297345795593088 1102 7 0.08961248193670457 1102 8 0.06810142160938426 1102 9 0.08919729231034523 1102 10-11 0.21061606562295054 1102 12-13 0.23105142996858774 1102 14-15 0.27216492469874254 1102 16-17 0.25366849780927936 1102 18-19 0.07803642230636143 1102 20-21 0.22713915393024564 1102 22-23 0.21695858765114195 1102 24-25 0.20494459900955775 1102 26-27 0.10484985008675096 1102 28-29 0.27482388929649915 1102 30-31 0.13360389205670486 1102 32-33 0.26487270599489676 1102 34-35 0.12690614951218748 1102 36-37 0.22415870509418312 1102 38-39 0.15405941387508904 1102 40-41 0.12317825950794514 1102 42-43 0.2481351381836987 1102 44-45 0.23354756006890653 1102 46-47 0.1077612155549943 1102 48-49 0.14953462249516036 1102 50-51 0.1740491343913746 1102 52-53 0.17800810454298244 1102 54-55 0.23998634408964392 1102 56-57 0.07736841861467525 1102 58-59 0.20465482749456498 1102 60-61 0.23522787192052874 1102 62-63 0.3272248263315234 1102 64-65 0.240838912915045 1102 66-67 -0.05429619777217454 1102 68-69 -0.09099036916573056 1102 70-71 0.08951882261707311 1102 72-73 0.26769333621051317 1102 74-75 0.06902217710805303 1102 76-77 -0.02711040560647504 1102 78-79 0.1581873860144789 1102 80-81 0.27711175231257457 1102 82-83 0.1997709379971404 1102 84-85 0.196357510134888 1102 86-87 0.12440694266454955 1102 88-89 0.38130722648051574 1102 90-91 0.5732265942602908 1102 92-93 0.04874123066952407 1102 94-95 -0.18987200507031687 1102 96-97 -0.02443544768905781 1102 98-99 0.2836564323780699 1102 100-101 0.6461334765178783 1103 1 0.1850691612812767 1103 2 0.0615147370683502 1103 3 0.14439516755530235 1103 4 0.13690839205407457 1103 5 0.18256572961629303 1103 6 0.05415672717425224 1103 7 0.016477889212829666 1103 8 -0.06647327157724447 1103 9 0.14576592506446673 1103 10-11 0.07229894399959136 1103 12-13 0.06619949904096956 1103 14-15 0.07291627408363865 1103 16-17 0.11070582239455007 1103 18-19 0.14728487140201452 1103 20-21 0.1599398395206464 1103 22-23 0.04786732373171532 1103 24-25 -0.02221078415847444 1103 26-27 0.0700055450796313 1103 28-29 0.1409083768590449 1103 30-31 0.01738019697472737 1103 32-33 0.1088849795904423 1103 34-35 0.11624577025256855 1103 36-37 -0.08679377131478105 1103 38-39 -0.12562625819760598 1103 40-41 -5.175649028501539E-4 1103 42-43 0.2515100587691208 1103 44-45 0.0894749366992258 1103 46-47 0.18804338521952246 1103 48-49 0.011165530064538132 1103 50-51 -0.04531805208943496 1103 52-53 -0.0800598329690203 1103 54-55 0.02952737822386098 1103 56-57 0.11496028231963606 1103 58-59 0.020702033957618937 1103 60-61 0.07678266877301354 1103 62-63 0.18050222210619182 1103 64-65 0.1838449680444043 1103 66-67 0.056108888154660974 1103 68-69 -0.07776220777622456 1103 70-71 -0.09895649177979493 1103 72-73 0.1201248805813826 1103 74-75 0.18943641626074026 1103 76-77 0.10297623715361937 1103 78-79 0.10924997689710025 1103 80-81 0.14547181879278526 1103 82-83 -0.05291589970651245 1103 84-85 0.13165809761713732 1103 86-87 -0.21618321236755023 1103 88-89 -0.1595248579624986 1103 90-91 -0.394581202845675 1103 92-93 -0.2079022824721939 1103 94-95 -0.34560836246825843 1103 96-97 -0.31527628231900806 1103 98-99 -0.03405649148894341 1103 100-101 0.40169567868007405 1104 1 0.1774188529565066 1104 2 0.16744784772268417 1104 3 0.11108324566423988 1104 4 0.10430733004653803 1104 5 0.03522824246315537 1104 6 -0.032529638019305196 1104 7 0.09233113518747871 1104 8 0.06822397062899199 1104 9 0.07868387641767072 1104 10-11 0.11080956407496245 1104 12-13 0.059985898492840306 1104 14-15 0.0627431625728434 1104 16-17 0.08320603650897596 1104 18-19 0.07949249815774806 1104 20-21 0.08507259046275095 1104 22-23 -0.04678302741594109 1104 24-25 0.057210143385198364 1104 26-27 0.08001432377096762 1104 28-29 0.09675048888369986 1104 30-31 0.08146089670066203 1104 32-33 -0.06976674188951648 1104 34-35 0.06918556127688902 1104 36-37 0.12663548527994806 1104 38-39 0.21583218219294054 1104 40-41 0.10343980491558113 1104 42-43 0.2977304039215696 1104 44-45 0.09204859206065663 1104 46-47 0.058273986452825 1104 48-49 0.1443778887490268 1104 50-51 0.1254810229362633 1104 52-53 0.11079480474938208 1104 54-55 0.00782206390388751 1104 56-57 0.05231843151457127 1104 58-59 0.17897113296412215 1104 60-61 0.28874470628585414 1104 62-63 0.3666388485193437 1104 64-65 0.22289515646509273 1104 66-67 0.034553453930605826 1104 68-69 -0.04251094450112447 1104 70-71 -0.06960140442111395 1104 72-73 -0.056458495574837286 1104 74-75 -0.22198498233467134 1104 76-77 -0.10436788754661919 1104 78-79 0.04218345154595582 1104 80-81 0.4103854236232074 1104 82-83 0.3654684999166449 1104 84-85 0.6540587485249816 1104 86-87 0.28574756443957483 1104 88-89 0.36869435238351045 1104 90-91 0.27637578156679155 1104 92-93 0.08687332561276762 1104 94-95 -0.015449275452159128 1104 96-97 0.07592736190846594 1104 98-99 -0.12917896493875247 1104 100-101 -0.007110847527531661 1105 1 0.12493262284635165 1105 2 0.09009421655173355 1105 3 0.0932894880143067 1105 4 0.09582609789639918 1105 5 0.013253793258748203 1105 6 0.12438080669713969 1105 7 0.11051631470604661 1105 8 0.12411697348497341 1105 9 0.12032504328833937 1105 10-11 0.17467635314064722 1105 12-13 0.19829169204648878 1105 14-15 0.1614178106756583 1105 16-17 -0.03592518544125056 1105 18-19 -0.03566266810091889 1105 20-21 0.01370886137258509 1105 22-23 0.05903985100103171 1105 24-25 0.038289932246890146 1105 26-27 0.11198979296720779 1105 28-29 0.09048136403670526 1105 30-31 0.007844898128652744 1105 32-33 -0.07540857175487758 1105 34-35 -0.05588838813151398 1105 36-37 -0.06328699534021354 1105 38-39 -0.06124286370668841 1105 40-41 -0.3174222156882536 1105 42-43 -0.049030484491318305 1105 44-45 -0.11107768292914244 1105 46-47 -0.11788497927545194 1105 48-49 -0.37732116265856774 1105 50-51 -0.24714749603558772 1105 52-53 -0.46115478215392613 1105 54-55 -0.514591619286648 1105 56-57 -0.45803129818759913 1105 58-59 -0.5493591403936904 1105 60-61 -0.6645849295770532 1105 62-63 -0.5773999366703748 1105 64-65 -0.7202453984104622 1105 66-67 -0.7325307718955756 1105 68-69 -0.6137541870551857 1105 70-71 -0.7407305353880673 1105 72-73 -0.3514057109971134 1105 74-75 -0.44774168878102216 1105 76-77 -0.36410370966004635 1105 78-79 -0.4447357906001095 1105 80-81 -0.466762296695034 1105 82-83 -0.25023746193566154 1105 84-85 -0.2575028589821571 1105 86-87 -0.26300995800024296 1105 88-89 -0.45134211232477384 1105 90-91 -0.487696918351606 1105 92-93 -0.46577865317140166 1105 94-95 -0.6363884329388974 1105 96-97 -0.6131147382547333 1105 98-99 -0.5644247829722069 1105 100-101 -0.23349548645041907 1106 1 0.14668698767153643 1106 2 0.035593357221820554 1106 3 0.1896454218300434 1106 4 0.12747226959749014 1106 5 0.10800997821444724 1106 6 0.11304462795651915 1106 7 -0.024211395037042394 1106 8 0.07762595681207074 1106 9 0.11894726156447888 1106 10-11 0.022230116751991602 1106 12-13 0.1975290763857558 1106 14-15 0.31205501490444476 1106 16-17 0.29341221094765757 1106 18-19 0.004327882440996689 1106 20-21 0.18401473208623997 1106 22-23 0.16063817292948102 1106 24-25 0.12426810970644198 1106 26-27 0.04801410788149951 1106 28-29 0.08782885675935148 1106 30-31 0.08110198046577466 1106 32-33 0.1660655119964929 1106 34-35 0.209287029325246 1106 36-37 -0.00829543009138689 1106 38-39 0.0810119101722151 1106 40-41 0.2861119767635927 1106 42-43 0.3430483011581842 1106 44-45 0.09915135544580522 1106 46-47 0.36243525588287895 1106 48-49 0.4549834586972068 1106 50-51 0.42826599702952706 1106 52-53 0.41075432547476254 1106 54-55 0.25957346718540464 1106 56-57 0.48195195914841804 1106 58-59 0.4730341726877896 1106 60-61 0.3773700301200549 1106 62-63 0.5326767371203829 1106 64-65 0.0376835847897965 1106 66-67 0.2985026119617018 1106 68-69 0.2375111841689943 1106 70-71 0.18590377692603255 1106 72-73 0.023868576964023447 1106 74-75 -0.01391881221377389 1106 76-77 0.100921404335935 1106 78-79 0.36879992391210337 1106 80-81 0.5102316023796796 1106 82-83 0.5808479256506658 1106 84-85 0.6887953633781763 1106 86-87 0.4623802285155705 1106 88-89 0.1464415458204691 1106 90-91 -0.06687497404633191 1106 92-93 0.23743463822070865 1106 94-95 0.3190984361195177 1106 96-97 0.3703709068134735 1106 98-99 -0.044053749049286495 1106 100-101 -0.01478571799384909 1107 1 0.08921562918912684 1107 2 0.12566266019634753 1107 3 0.06384873238990707 1107 4 0.06338339410047666 1107 5 -0.013661612151260272 1107 6 0.0667170960725656 1107 7 0.0512030799830967 1107 8 0.06921691205334923 1107 9 0.0944603197383671 1107 10-11 0.10274487802355026 1107 12-13 0.03900507253413821 1107 14-15 0.1497473503098803 1107 16-17 0.12638581948410632 1107 18-19 0.1351110453553943 1107 20-21 0.0762283005343889 1107 22-23 0.13433923343530552 1107 24-25 0.1432977698074538 1107 26-27 0.1351357418072041 1107 28-29 0.06634198698736782 1107 30-31 -0.027822578857701785 1107 32-33 -0.09917966856460936 1107 34-35 0.017650057694929444 1107 36-37 -0.2941399045219981 1107 38-39 -0.34534631022172135 1107 40-41 -0.19497135711560531 1107 42-43 -0.0750367947720676 1107 44-45 -0.22836333377162532 1107 46-47 -0.1976718100073569 1107 48-49 0.004053141593523435 1107 50-51 -0.23047662560988158 1107 52-53 -0.4440467723681891 1107 54-55 -0.27985242513951647 1107 56-57 -0.2414322269346556 1107 58-59 -0.1826649816587178 1107 60-61 -0.14537942456117747 1107 62-63 -0.2661776021759721 1107 64-65 -0.4592213627089521 1107 66-67 -0.45995310422362223 1107 68-69 -0.28095186843707864 1107 70-71 -0.36060962186738266 1107 72-73 -0.7139845701639622 1107 74-75 -0.5861686369912249 1107 76-77 -0.6311641370198622 1107 78-79 -0.31916228697069116 1107 80-81 -0.2857053633553015 1107 82-83 -0.37455138524980924 1107 84-85 -0.3303333711547509 1107 86-87 -0.35720768735140496 1107 88-89 -0.27928947610363863 1107 90-91 -0.348864552398652 1107 92-93 -0.10577311349384999 1107 94-95 -0.11951717473576551 1107 96-97 -0.011512719212479539 1107 98-99 -0.428425699030619 1107 100-101 -0.11140130264763215 1108 1 0.13238055212085698 1108 2 0.16730276879975037 1108 3 0.08946068299015053 1108 4 0.14979247396482975 1108 5 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-0.9170997602906041 2316 70-71 -1.0205029638753054 2316 72-73 -0.8744348600972529 2316 74-75 -1.1153603478317464 2316 76-77 -0.8983981019715728 2316 78-79 -0.9008081420212939 2316 80-81 -0.6726646494762072 2316 82-83 -0.4785343022276045 2316 84-85 -0.7147765341260879 2316 86-87 -0.6310683468664671 2316 88-89 -0.9399862031720474 2316 90-91 -0.7110297983552343 2316 92-93 -0.5752124443677396 2316 94-95 -0.9002263222357847 2316 96-97 -0.24734566440732664 2316 98-99 -0.7322247739056174 2316 100-101 -1.045816987878407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 10.0 6 26.0 7 58.0 8 155.0 9 351.0 10 564.0 11 1012.0 12 1671.0 13 2501.0 14 3149.0 15 4500.0 16 6572.0 17 10048.0 18 9334.0 19 4985.0 20 3712.0 21 3710.0 22 3784.0 23 4065.0 24 4508.0 25 5280.0 26 6140.0 27 7781.0 28 9541.0 29 12001.0 30 15263.0 31 19370.0 32 25557.0 33 35058.0 34 49845.0 35 74819.0 36 118573.0 37 178607.0 38 127807.0 39 8844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.597343733530096 19.639638452619373 15.28104247918204 20.481975334668494 2 15.584227407744034 22.002068061620335 39.559647770906196 22.854056759729442 3 20.848711403349323 25.788661192468894 30.22373610046391 23.13889130371787 4 12.927801728396037 17.63011376433909 38.445549992689685 30.996534514575192 5 13.173683454886243 38.47218728060874 33.801368013561586 14.552761250943425 6 29.9470629067588 37.93557190896242 17.167476946218603 14.949888238060177 7 27.138064522927337 34.430843924483966 20.813800788458423 17.617290764130278 8 26.0990802196514 33.81638376033814 19.747946201477077 20.336589818533383 9 26.220128213402738 17.916947114276418 20.825391891233306 35.037532781087535 10-11 24.754682730902534 27.20699916613864 26.943733459444434 21.094584643514388 12-13 26.972035147384823 24.909082287609507 27.45741257608308 20.661469988922594 14-15 23.037711916312237 26.15150361629235 27.722098042289083 23.088686425106328 16-17 21.007095598937305 29.34221808923305 27.885032066522392 21.76565424530725 18-19 20.753092883650936 27.987658101758093 30.98646276849721 20.272786246093762 20-21 22.318083867670534 25.9755056665209 30.971448743144066 20.7349617226645 22-23 21.537028828922576 26.253201622001477 31.130599833637497 21.079169715438454 24-25 22.120502560555913 26.2331340875035 31.22429152464226 20.422071827298325 26-27 21.629258069593888 26.033393421557864 31.878526505888406 20.458822002959845 28-29 20.7472587975656 27.500209101272848 31.38929520028609 20.36323690087546 30-31 21.347901683395243 26.377275481440503 31.120221818277617 21.15460101688664 32-33 21.403050286857635 26.72350032831714 31.09601284026268 20.777436544562548 34-35 22.107394201550836 26.303570981674202 31.063167558610804 20.525867258164155 36-37 21.911713466920375 25.992698225140476 31.54101962706017 20.55456868087898 38-39 21.287264684398316 26.78189756867715 31.48251216715287 20.448325579771666 40-41 21.370968007497428 26.98027577949495 30.945931439086344 20.70282477392127 42-43 21.251430776748776 27.287760980923416 30.13614277077409 21.324665471553722 44-45 21.907513537222588 26.49330943107443 30.107626023606333 21.49155100809665 46-47 22.50169750941945 27.34251360477239 29.528760847760243 20.62702803804792 48-49 22.380659773744064 27.457093763110286 29.164254538916428 20.997991924229222 50-51 21.49617407592309 27.773943145279734 29.80308576682478 20.926797011972393 52-53 22.17878485727796 28.46669467849837 28.334977601511056 21.01954286271261 54-55 21.64951644813969 27.782102653673597 28.734453498144035 21.833927400042676 56-57 22.33128769707196 27.760258806576065 28.586614188786015 21.321839307565956 58-59 22.96022665904463 27.48471421308163 28.31841855000382 21.236640577869924 60-61 21.974215855766857 27.829159463241954 29.588145221042232 20.60847945994896 62-63 21.683223027116956 27.771349011327434 29.447577599089534 21.097850362466072 64-65 21.44083993345653 28.657223430360524 28.775571224033627 21.12636541214932 66-67 21.923781624290196 27.753570595913786 28.389909205505365 21.932738574290656 68-69 21.681008536628738 27.922981984960465 28.63234804050058 21.763661437910216 70-71 22.431785057814185 27.920790253418236 28.38799287934199 21.259431809425585 72-73 22.24991207885111 27.311799347473602 29.074503130256335 21.363785443418955 74-75 21.549440663432573 28.890823666397523 28.21998859330113 21.339747076868772 76-77 21.984502168721672 28.040326500290437 27.96998958117921 22.00518174980868 78-79 22.092610484456802 28.151893365367453 28.010558614735036 21.74493753544071 80-81 22.544781339034152 28.185076443415273 27.843922923013903 21.42621929453667 82-83 22.30088926916052 28.01267015893027 28.334298283574178 21.352142288335035 84-85 21.82369875762284 28.498602712640924 28.116793859444023 21.560904670292217 86-87 21.779414650981135 28.57860745471047 27.798677768249842 21.84330012605856 88-89 21.680762589188927 28.584844896555992 27.328395699174003 22.405996815081078 90-91 22.023328519051972 27.755813853934306 27.756933454250166 22.463924172763548 92-93 22.379500040503082 28.256595910308395 27.721889379828674 21.642014669359845 94-95 22.048648385214495 28.20510456360157 27.75805680430662 21.988190246877316 96-97 21.194049706957223 28.28595495591022 28.614331467966824 21.905663869165735 98-99 21.649303931938256 28.813226676220673 27.19809060410199 22.339378787739086 100-101 21.20511223012675 28.80325184832962 27.12852624937599 22.86310967216764 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 360.0 1 267.5 2 212.5 3 264.0 4 236.0 5 207.5 6 342.5 7 733.0 8 1243.0 9 1519.0 10 1437.5 11 1290.0 12 1293.0 13 1384.5 14 1530.5 15 1680.0 16 1743.0 17 1781.5 18 1901.5 19 2000.5 20 2122.0 21 2345.5 22 2669.0 23 3067.0 24 3527.0 25 4261.0 26 5217.0 27 6313.5 28 7499.0 29 8741.0 30 10173.5 31 11652.5 32 13116.0 33 14621.5 34 16301.5 35 17703.5 36 19515.5 37 21137.5 38 21950.0 39 22285.0 40 22716.0 41 23643.0 42 23888.0 43 24351.5 44 25487.5 45 26248.0 46 26879.0 47 31306.0 48 39822.0 49 37913.5 50 28654.0 51 24707.0 52 22809.0 53 21974.5 54 20981.0 55 18983.0 56 17101.5 57 15524.0 58 14189.5 59 12671.5 60 10571.0 61 8511.5 62 6719.5 63 5096.5 64 3683.0 65 2658.5 66 1877.0 67 1387.0 68 1030.0 69 670.0 70 465.0 71 314.0 72 240.0 73 197.5 74 162.5 75 128.0 76 76.0 77 37.5 78 27.0 79 15.5 80 7.5 81 4.0 82 3.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03200724970791738 2 0.005005248925517944 3 6.585853849365716E-4 4 2.634341539746286E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011064234466934404 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.585853849365715E-5 20-21 9.220195389112002E-4 22-23 0.002305048847278001 24-25 0.011920395467351947 26-27 0.005466258694973545 28-29 1.975756154809715E-4 30-31 0.0030294927707082294 32-33 0.0037539366941384588 34-35 0.0 36-37 0.00954948808158029 38-39 0.011788678390364633 40-41 0.001251312231379486 42-43 0.0 44-45 0.0 46-47 3.292926924682858E-4 48-49 0.0024367659242653153 50-51 0.005268683079492573 52-53 0.0 54-55 0.0038197952326321154 56-57 0.003424644001670173 58-59 1.317170769873143E-4 60-61 0.0020416146933033724 62-63 0.0037539366941384588 64-65 0.0 66-67 0.002107473231797029 68-69 5.268683079492572E-4 70-71 4.610097694556001E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0016464634623414292 80-81 0.0018440390778224004 82-83 0.012710697929275833 84-85 0.007046863618821317 86-87 0.004214946463594058 88-89 0.0 90-91 4.610097694556001E-4 92-93 1.975756154809715E-4 94-95 0.0 96-97 0.0017781805393287434 98-99 0.0013171707698731432 100-101 5.268683079492572E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 759203.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.21299446612946 #Duplication Level Percentage of deduplicated Percentage of total 1 85.10393647147039 57.20090411102772 2 9.429437626515188 12.675614780193564 3 2.4310041823379755 4.9018521196385985 4 0.9738470565564937 2.618207072927523 5 0.5373412285779099 1.8058156511415129 6 0.32243804284957606 1.3003215833830901 7 0.23358223133258582 1.0989832855360282 8 0.16584032306080682 0.8917299772917704 9 0.11985964627903324 0.7250513167858406 >10 0.6279008921310844 7.544871091994687 >50 0.03272995890518708 1.5456054961007402 >100 0.018139064992826817 2.3986058497278466 >500 9.85818747724952E-4 0.423665353246628 >1k 0.002563128744084875 3.2208903380229263 >5k 3.9432749908998075E-4 1.6478819729814889 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7143 0.9408550809203862 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5292 0.6970467714168674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4398 0.5792917045902084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2810 0.37012498633435326 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2456 0.323497141080844 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1768 0.23287579211357176 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1724 0.2270802407261299 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1599 0.2106156061027156 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1583 0.20850813287091857 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1524 0.20073682532866705 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1517 0.19981480578975583 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1381 0.1819012833194811 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1271 0.1674124048508765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 0.16240715592535857 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1041 0.13711747714379421 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.585853849365717E-5 0.0 18-19 0.0 0.0 0.0 1.3171707698731434E-4 0.0 20-21 0.0 0.0 0.0 1.3171707698731434E-4 0.0 22-23 0.0 0.0 0.0 2.634341539746287E-4 0.0 24-25 0.0 0.0 0.0 5.268683079492573E-4 0.0 26-27 0.0 0.0 0.0 9.220195389112003E-4 0.0 28-29 0.0 0.0 0.0 0.002305048847278001 0.0 30-31 0.0 0.0 0.0 0.005466258694973545 0.0 32-33 0.0 0.0 0.0 0.00928605392760566 0.0 34-35 0.0 0.0 0.0 0.01277655646776949 0.0 36-37 0.0 0.0 0.0 0.01574019069998406 0.0 38-39 0.0 0.0 0.0 0.018440390778224007 0.0 40-41 0.0 0.0 0.0 0.022260186010856123 0.0 42-43 0.0 0.0 0.0 0.028714322783234526 0.0 44-45 0.0 0.0 0.0 0.03832966940330847 0.0 46-47 0.0 0.0 0.0 0.06572682141666986 0.0 48-49 0.0 0.0 0.0 0.11973082298146873 0.0 50-51 0.0 0.0 0.0 0.15312110199775292 0.0 52-53 0.0 0.0 0.0 0.18644552247554344 0.0 54-55 0.0 0.0 0.0 0.23287579211357173 0.0 56-57 0.0 0.0 0.0 0.27021758343947533 0.0 58-59 0.0 0.0 0.0 0.3132890676143271 0.0 60-61 0.0 0.0 0.0 0.38211124034019883 0.0 62-63 0.0 0.0 0.0 0.4303855490560496 0.0 64-65 0.0 0.0 0.0 0.48840692146896153 0.0 66-67 0.0 0.0 0.0 0.6022763345244948 0.0 68-69 0.0 0.0 0.0 0.7210851379670523 0.0 70-71 0.0 0.0 0.0 0.8240220336326385 0.0 72-73 0.0 0.0 0.0 0.9417112419208038 0.0 74-75 0.0 0.0 0.0 1.0414210692002008 0.0 76-77 0.0 0.0 0.0 1.1117579883114266 0.0 78-79 0.0 0.0 0.0 1.2175926596707338 0.0 80-81 0.0 0.0 0.0 1.3639961907421334 0.0 82-83 0.0 0.0 0.0 1.5241641563587076 0.0 84-85 0.0 0.0 0.0 1.7089632153719099 0.0 86-87 0.0 0.0 0.0 2.0672995233158984 0.0 88-89 0.0 0.0 0.0 2.543983624932989 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATTGCG 30 0.003949705 31.669357 76-77 GTCGTAA 30 0.003950979 31.667273 38-39 TAAGGTG 315 0.0 27.144268 5 GGGCGTA 115 1.2807404E-4 24.783897 9 CCTACGT 100 0.0016538973 23.748108 2 GTAAGGT 260 2.3646862E-11 23.748108 4 GAGTACT 4640 0.0 23.54338 12-13 TGTATCG 105 0.0021948759 22.620224 9 GTACATG 3710 0.0 22.281721 1 TACATGG 3705 0.0 21.66494 2 GTACTTT 5050 0.0 21.492277 14-15 AGTACTT 4885 0.0 21.195854 12-13 ACATGGG 3710 0.0 20.99563 3 ACTTTTT 5545 0.0 19.616499 16-17 CATGGGA 2320 0.0 19.44888 4 AGAGTAC 7215 0.0 19.356516 10-11 TACTTTT 5335 0.0 19.097673 14-15 GGTAAGG 335 7.3123374E-10 18.431366 3 CGCGGGG 80 0.0011918909 17.81108 88-89 AAGGTGT 510 0.0 17.696999 6 >>END_MODULE