FastQCFastQC Report
Thu 26 May 2016
SRR936920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784868
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT45270.5767848861209783No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43660.5562718826605237No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39300.5007211403701004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.433576091775942No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25750.3280806454078902No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22560.2874368683651264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20370.25953408726053295No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16280.20742341387341565No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16060.20462039476701813No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC12800.16308474800858233No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC12390.15786093967393242No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10480.13352563743202678No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9330.11887349210313071No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8760.11161112441837355No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8340.10625990612434191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG38200.027.380381
TACATGG37950.027.1849232
TACACCG700.00835362227.1670285
ACATGGG38900.025.2988573
GAGTACT34050.024.91828512-13
GTACTTT37150.022.645614-15
CATGGGA24000.022.1864054
AGTACTT36500.021.94340912-13
GTGTAGC4250.021.2542041
AGAGTAC58100.020.7355610-11
CATGGGG13950.020.4483014
ATGGGAT11200.020.375275
GCACCGT1707.907173E-519.5724966
ACTTTTT44550.019.25561116-17
GTATCAA96500.019.016921
GCTACAC3759.094947E-1219.016923
TACTTTT42950.018.70220814-15
ATGGGAG10200.018.1779385
GTATAGA2101.9395975E-518.1113531
TACCTGG6350.017.2200452
Produced by FastQC (version 0.11.5)