##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936920_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784868 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.906949958464352 33.0 31.0 34.0 30.0 34.0 2 32.00501867830005 33.0 31.0 34.0 30.0 34.0 3 32.022022811479125 34.0 31.0 34.0 30.0 34.0 4 35.60442265451006 37.0 35.0 37.0 33.0 37.0 5 35.59853376618744 37.0 35.0 37.0 33.0 37.0 6 35.67529826671491 37.0 35.0 37.0 33.0 37.0 7 35.63688798626011 37.0 35.0 37.0 33.0 37.0 8 35.60592864022995 37.0 35.0 37.0 33.0 37.0 9 37.251099547949465 39.0 37.0 39.0 34.0 39.0 10-11 37.13005944948705 39.0 37.0 39.0 33.5 39.0 12-13 37.06940084192502 39.0 37.0 39.0 33.0 39.0 14-15 38.21878901930006 40.0 38.0 41.0 33.0 41.0 16-17 38.164703491542525 40.0 38.0 41.0 33.0 41.0 18-19 38.14665395964672 40.0 38.0 41.0 33.5 41.0 20-21 38.08718982045389 40.0 38.0 41.0 33.0 41.0 22-23 37.975533465499936 40.0 38.0 41.0 33.0 41.0 24-25 37.94045495548296 40.0 38.0 41.0 33.0 41.0 26-27 37.94991132266827 40.0 38.0 41.0 33.0 41.0 28-29 37.87605941381226 40.0 38.0 41.0 33.0 41.0 30-31 37.715345765147774 40.0 37.0 41.0 33.0 41.0 32-33 37.491626617469436 40.0 37.0 41.0 32.5 41.0 34-35 37.30367399358873 40.0 36.5 41.0 32.0 41.0 36-37 37.10964264054593 39.5 36.0 41.0 31.0 41.0 38-39 36.93656767762222 39.0 36.0 41.0 31.0 41.0 40-41 36.76553637044701 39.0 36.0 41.0 30.5 41.0 42-43 36.532688936228766 39.0 35.5 41.0 30.0 41.0 44-45 36.24286249407544 39.0 35.0 41.0 30.0 41.0 46-47 35.85141450536906 39.0 35.0 40.0 28.5 41.0 48-49 35.562870444456905 39.0 35.0 40.0 27.0 41.0 50-51 34.87789029492858 38.0 34.0 39.5 26.5 40.5 52-53 35.079496807106416 38.5 35.0 40.0 27.0 40.5 54-55 35.46812776161087 39.0 35.0 40.5 27.0 41.0 56-57 35.31336033575073 39.0 35.0 41.0 26.5 41.0 58-59 35.095614804018 39.0 35.0 40.5 26.0 41.0 60-61 34.7890906496379 38.0 34.5 40.0 26.0 41.0 62-63 34.51273207724101 38.0 34.0 40.0 25.5 41.0 64-65 34.188968081256974 37.5 34.0 40.0 24.5 41.0 66-67 33.790733601064126 37.0 34.0 40.0 23.0 41.0 68-69 33.38241143733723 36.0 33.0 39.0 22.0 41.0 70-71 32.92844274451245 36.0 33.0 39.0 21.5 40.5 72-73 32.537308184306156 35.0 32.5 38.5 20.5 40.0 74-75 32.15206760882084 35.0 32.0 37.5 19.5 40.0 76-77 31.708794345036363 35.0 32.0 37.0 18.0 39.0 78-79 31.21751937905482 35.0 31.0 36.5 14.5 39.0 80-81 30.736219338793276 35.0 31.0 36.0 7.5 38.5 82-83 30.300312918860243 34.5 30.5 35.5 7.0 37.0 84-85 29.918274792703997 34.0 30.0 35.0 3.5 37.0 86-87 29.595156128164227 34.0 30.0 35.0 2.0 36.5 88-89 29.205948006543775 34.0 29.5 35.0 2.0 36.0 90-91 28.812985113420346 34.0 29.0 35.0 2.0 36.0 92-93 28.397615904840052 34.0 29.0 35.0 2.0 35.0 94-95 28.028423887838464 34.0 29.0 35.0 2.0 35.0 96-97 27.539150532318807 34.0 27.0 35.0 2.0 35.0 98-99 26.984717812421962 34.0 26.5 35.0 2.0 35.0 100-101 24.84315069540356 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1564868345681063 1101 2 0.11302099122630338 1101 3 0.07436948492140161 1101 4 0.09219082229989795 1101 5 0.05805064617081257 1101 6 -0.05991181082465147 1101 7 -0.25836076666411145 1101 8 -0.09687225547690304 1101 9 -0.15627741442514775 1101 10-11 -0.09174143645358157 1101 12-13 0.06711111730695762 1101 14-15 0.0624682247824353 1101 16-17 0.16694755878032908 1101 18-19 0.11909346255190911 1101 20-21 0.002009647868710829 1101 22-23 0.14690095413826754 1101 24-25 0.05571948996193754 1101 26-27 -0.06955603565315727 1101 28-29 -0.07535894603464754 1101 30-31 -0.016344721838216003 1101 32-33 -0.05205119435363059 1101 34-35 -0.1698212356544957 1101 36-37 -0.10867609178999516 1101 38-39 -0.11142652675647469 1101 40-41 0.05724779496986798 1101 42-43 0.003720572277401857 1101 44-45 0.05524038276533361 1101 46-47 -0.02915335728766877 1101 48-49 0.018368192911353276 1101 50-51 0.10128841851504689 1101 52-53 0.14857090893781333 1101 54-55 0.042903623312319894 1101 56-57 0.1277945806170706 1101 58-59 -0.010404600758029403 1101 60-61 0.1894564918867232 1101 62-63 0.1587967345770096 1101 64-65 0.15065953679547306 1101 66-67 0.13969616853639621 1101 68-69 0.20617289789617388 1101 70-71 0.2210287677740368 1101 72-73 0.22352578932712674 1101 74-75 0.3681730077048968 1101 76-77 0.35222453625983974 1101 78-79 0.407016613263405 1101 80-81 0.43229023982542003 1101 82-83 0.2846544950945109 1101 84-85 0.13462035109644077 1101 86-87 0.46364161199929654 1101 88-89 0.3833483297897935 1101 90-91 0.38312482290107397 1101 92-93 0.2954110939533301 1101 94-95 0.449844202568233 1101 96-97 0.4280748936573353 1101 98-99 0.13564331517816086 1101 100-101 0.3119890379362751 1102 1 0.13515274170123703 1102 2 0.09713794086552596 1102 3 3.1496523373419905E-4 1102 4 -0.004654334747556277 1102 5 0.07119196572138975 1102 6 0.02560518142254864 1102 7 -0.055581522307349474 1102 8 0.008651961601486846 1102 9 -0.003437500011607142 1102 10-11 0.009326310232715684 1102 12-13 -0.07001942057245714 1102 14-15 -0.22603294693698217 1102 16-17 0.05766967139506818 1102 18-19 -0.00945643046500777 1102 20-21 0.04732693837176072 1102 22-23 0.1472100935497238 1102 24-25 -0.12555430335231677 1102 26-27 0.06900789020895814 1102 28-29 -0.1550694433438622 1102 30-31 -0.2174462770294454 1102 32-33 -0.22861032218683164 1102 34-35 -0.14993210902543552 1102 36-37 -0.04734839011995007 1102 38-39 0.03888238108987707 1102 40-41 -0.03698878120115978 1102 42-43 -0.22382340193551187 1102 44-45 0.0753680214496697 1102 46-47 -0.030113194636342655 1102 48-49 0.17911489773994305 1102 50-51 0.2034294694501071 1102 52-53 0.353211936716356 1102 54-55 -0.019728947708699707 1102 56-57 0.24413057231777202 1102 58-59 -0.08184706609274173 1102 60-61 0.0374316866329778 1102 62-63 -0.09245603262596092 1102 64-65 0.2147116018930575 1102 66-67 -0.1751200613984949 1102 68-69 0.08530865062169823 1102 70-71 0.03645632957876188 1102 72-73 0.036430449343249904 1102 74-75 0.25786581344030424 1102 76-77 0.24890250330349772 1102 78-79 0.19304582751670196 1102 80-81 0.3237336777335571 1102 82-83 0.41693258625706164 1102 84-85 0.3140416235929884 1102 86-87 0.36086723050968317 1102 88-89 0.3769015573441905 1102 90-91 0.3917703059921891 1102 92-93 0.42983926708992115 1102 94-95 0.2998814845496902 1102 96-97 0.038515237855701656 1102 98-99 -0.04117516556951628 1102 100-101 0.10289848671870416 1103 1 0.1288777618873702 1103 2 0.030902979324537228 1103 3 0.028432269006813726 1103 4 0.07607250092635809 1103 5 0.03821060439212687 1103 6 -0.012001712373944429 1103 7 0.16760294777081697 1103 8 -0.004021053372497363 1103 9 -0.1516079367232308 1103 10-11 0.12316978383228872 1103 12-13 -0.07221081714950373 1103 14-15 0.160084808600935 1103 16-17 0.0903210589705239 1103 18-19 0.14636324456773764 1103 20-21 0.12568875143215053 1103 22-23 0.06200817321204255 1103 24-25 0.14239576314814428 1103 26-27 0.1666434575868223 1103 28-29 0.14995020336930054 1103 30-31 0.058142070809246604 1103 32-33 0.15998371148313595 1103 34-35 0.11722189052616727 1103 36-37 -0.022588137333926284 1103 38-39 0.11801455433158026 1103 40-41 0.09221891093796586 1103 42-43 0.11409964295946651 1103 44-45 0.13125116269866055 1103 46-47 0.15237198393719353 1103 48-49 0.20370068164464072 1103 50-51 0.3085490775609685 1103 52-53 0.21661923811261374 1103 54-55 0.16809059584024766 1103 56-57 0.3661555407532333 1103 58-59 0.26363587557059276 1103 60-61 0.2411624550208984 1103 62-63 0.24970567895591245 1103 64-65 0.3726257463465501 1103 66-67 0.269505875634259 1103 68-69 0.10951051870299722 1103 70-71 -0.04035802036030134 1103 72-73 -0.019121032462258825 1103 74-75 0.0011854252596847914 1103 76-77 -0.06784954354519002 1103 78-79 0.08400867660196099 1103 80-81 -0.008512807182050608 1103 82-83 -0.23871304131677817 1103 84-85 -0.3146182194018756 1103 86-87 -0.2822200089880482 1103 88-89 -0.30739910900894074 1103 90-91 -0.01801748253466684 1103 92-93 0.027975905723572225 1103 94-95 0.26063525754460315 1103 96-97 0.23101552020872163 1103 98-99 0.28700080020424323 1103 100-101 0.23867404725266184 1104 1 0.19154374459588652 1104 2 0.13745605239025238 1104 3 0.22858696741559825 1104 4 0.2967469536756866 1104 5 0.24009707676351155 1104 6 0.15694369282076792 1104 7 0.2862249201957212 1104 8 0.16109829261272068 1104 9 0.27572925127976333 1104 10-11 0.4648083954241642 1104 12-13 0.2485379554620195 1104 14-15 0.2829261498321003 1104 16-17 0.36416198215246 1104 18-19 0.4277351124803417 1104 20-21 0.45897294210298867 1104 22-23 0.15314542866840242 1104 24-25 0.3138176555984131 1104 26-27 0.18968513931604747 1104 28-29 0.3024523390555558 1104 30-31 0.3880091634687446 1104 32-33 0.16775245957718 1104 34-35 0.49353384814769186 1104 36-37 0.3246502133306066 1104 38-39 0.4434194259177673 1104 40-41 0.3676058084270437 1104 42-43 0.3579753227943385 1104 44-45 0.34278306066234165 1104 46-47 0.3598364850954141 1104 48-49 0.29691152184280867 1104 50-51 0.10643843386613838 1104 52-53 0.23131609183779744 1104 54-55 0.36238351707533667 1104 56-57 0.4986816890253962 1104 58-59 0.18411968171692905 1104 60-61 0.19872540980090037 1104 62-63 0.10687673783218088 1104 64-65 0.23691373985163722 1104 66-67 -0.03755027019423807 1104 68-69 -0.06411875443663462 1104 70-71 0.14616766745184862 1104 72-73 0.18841061911491153 1104 74-75 0.12954622799497884 1104 76-77 0.27740458797268985 1104 78-79 0.4071405317509367 1104 80-81 0.24729242227042647 1104 82-83 0.30114906302588906 1104 84-85 0.5814223293533693 1104 86-87 0.8769860610059119 1104 88-89 0.6467416988560402 1104 90-91 0.7462027546830221 1104 92-93 0.8045366733184984 1104 94-95 0.7044823605846524 1104 96-97 0.8598004308532232 1104 98-99 0.8237275353609697 1104 100-101 0.8586198422227405 1105 1 0.10009284893410353 1105 2 0.13367366407109316 1105 3 0.2239470650323092 1105 4 0.13172212851399934 1105 5 0.051999682854969365 1105 6 0.02375958247865384 1105 7 0.009987515743127062 1105 8 0.04588829881738832 1105 9 0.02580664775307895 1105 10-11 0.16352950775721808 1105 12-13 0.14175413838453466 1105 14-15 0.14413451927298837 1105 16-17 0.29694557384105025 1105 18-19 0.1302820069711359 1105 20-21 0.07645107355134684 1105 22-23 0.2278942301564868 1105 24-25 0.09683874759464572 1105 26-27 0.3469586591004017 1105 28-29 0.3014957315988127 1105 30-31 0.3826296228216748 1105 32-33 0.2629028418963202 1105 34-35 0.054901803287535245 1105 36-37 0.06560777933781736 1105 38-39 0.43374324758075034 1105 40-41 0.44244852195387807 1105 42-43 0.5016348353066675 1105 44-45 0.29730372713989084 1105 46-47 0.3792432459850019 1105 48-49 0.47003026940594594 1105 50-51 0.569668956185474 1105 52-53 0.5184984586543351 1105 54-55 0.3939820036018702 1105 56-57 0.5485489815996161 1105 58-59 0.28783872798512533 1105 60-61 0.5832343275585643 1105 62-63 0.44360450561426745 1105 64-65 0.5185850891668622 1105 66-67 0.5966338414432144 1105 68-69 0.4376493825687291 1105 70-71 0.3218900453305764 1105 72-73 0.5101882379978377 1105 74-75 0.5145111048924846 1105 76-77 0.3289914423598077 1105 78-79 0.5613952652415186 1105 80-81 0.08059466748058952 1105 82-83 0.18406924815565873 1105 84-85 0.26902555163733766 1105 86-87 0.4695483570710657 1105 88-89 0.46680882329532025 1105 90-91 0.4423085786542984 1105 92-93 0.50466711390197 1105 94-95 0.4605085989606046 1105 96-97 0.4978796252563278 1105 98-99 0.7025233628167022 1105 100-101 0.6858352919836896 1106 1 0.187220677822733 1106 2 0.4209829688341067 1106 3 0.3727427251386679 1106 4 0.33614936348158864 1106 5 0.3486642145113521 1106 6 0.3316329181410893 1106 7 0.3041045919435845 1106 8 0.27992909692305545 1106 9 0.38084430157086757 1106 10-11 0.5326558033202744 1106 12-13 0.5372502371384584 1106 14-15 0.5845070785680448 1106 16-17 0.6995866786118299 1106 18-19 0.6530004573418466 1106 20-21 0.38253148580242424 1106 22-23 0.41343875482555603 1106 24-25 0.29792517669289964 1106 26-27 0.42269814902703473 1106 28-29 0.4433404775325869 1106 30-31 0.4832880794033301 1106 32-33 0.6781477858037803 1106 34-35 0.6896524923135772 1106 36-37 0.45288166233925864 1106 38-39 0.33731114090787173 1106 40-41 0.4628500009337273 1106 42-43 0.36180864455320005 1106 44-45 0.6446844577015867 1106 46-47 1.0454780582981869 1106 48-49 1.0419728376292383 1106 50-51 1.2743291013661704 1106 52-53 1.1587831913898654 1106 54-55 1.3368704784281604 1106 56-57 1.259473441053828 1106 58-59 1.3584153748544239 1106 60-61 1.3901202209633183 1106 62-63 1.231044822372155 1106 64-65 0.9668038112661534 1106 66-67 0.9554247462993857 1106 68-69 1.0661454619134716 1106 70-71 1.125473951234845 1106 72-73 1.2761668490766027 1106 74-75 1.592392883550147 1106 76-77 1.5651721030484467 1106 78-79 1.416272793287792 1106 80-81 1.5729620057050369 1106 82-83 1.4276472342586857 1106 84-85 1.5397375775680082 1106 86-87 1.6186618425724042 1106 88-89 1.5292578741862002 1106 90-91 1.301897686219995 1106 92-93 1.644090564078592 1106 94-95 1.6311226085099229 1106 96-97 1.8045229038557586 1106 98-99 1.6339943849043586 1106 100-101 1.8790755582776981 1107 1 0.2606438843706407 1107 2 0.3779361063722213 1107 3 0.38075508270567227 1107 4 0.33800299853214 1107 5 0.24111727180552833 1107 6 0.32613219594561826 1107 7 0.4874539740652324 1107 8 0.35061197286592005 1107 9 0.3125767682696292 1107 10-11 0.5558081865653364 1107 12-13 0.5367808100802094 1107 14-15 0.496846590684882 1107 16-17 0.6871147639913815 1107 18-19 0.7565953859247401 1107 20-21 0.7867021646661669 1107 22-23 0.8225805458703306 1107 24-25 0.6640361540641067 1107 26-27 0.7320826779950949 1107 28-29 0.7026748540371628 1107 30-31 0.7574756094948114 1107 32-33 0.9212267458422971 1107 34-35 0.8655893403315531 1107 36-37 0.8280321197297269 1107 38-39 0.8981621279083498 1107 40-41 0.8831376754643046 1107 42-43 0.8270690361436337 1107 44-45 0.9491420086564943 1107 46-47 1.065454787555133 1107 48-49 0.9110247554594366 1107 50-51 0.7649084626243976 1107 52-53 0.9518380782459062 1107 54-55 1.020332202068026 1107 56-57 0.9432200306863123 1107 58-59 1.0649351951079993 1107 60-61 1.075377402796093 1107 62-63 1.057075957021489 1107 64-65 0.9372920956506832 1107 66-67 0.7407822330591358 1107 68-69 0.7543636451906366 1107 70-71 0.7577280035539005 1107 72-73 0.5905104536344581 1107 74-75 0.7317866405714923 1107 76-77 0.9317906233501674 1107 78-79 0.9038751043133111 1107 80-81 0.8132885985472775 1107 82-83 0.8470602498260078 1107 84-85 0.9575136167270522 1107 86-87 0.9246420223188316 1107 88-89 0.9837996812797662 1107 90-91 1.1618920691440877 1107 92-93 1.287789408565832 1107 94-95 1.4228233788517635 1107 96-97 1.2028197261956741 1107 98-99 1.3159274614570755 1107 100-101 1.4738536837792218 1108 1 0.08587193850741315 1108 2 0.055504323873268646 1108 3 0.12866135692710756 1108 4 -0.02239408396482645 1108 5 -0.051119944244717885 1108 6 -0.050185947027927114 1108 7 -0.0064526578881825 1108 8 0.006968671397231674 1108 9 -0.045503005961741394 1108 10-11 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-2.9482120346912453 2316 76-77 -2.860640711101837 2316 78-79 -3.2758917419288522 2316 80-81 -3.3666813740748225 2316 82-83 -4.007474767395578 2316 84-85 -3.8532962393181656 2316 86-87 -4.258028206419137 2316 88-89 -3.85803772930047 2316 90-91 -4.604700265042965 2316 92-93 -4.98652835174504 2316 94-95 -4.960915471276291 2316 96-97 -5.269453522943248 2316 98-99 -5.3596112650288354 2316 100-101 -5.309132274005815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1644.0 3 521.0 4 506.0 5 526.0 6 577.0 7 706.0 8 819.0 9 1102.0 10 1365.0 11 1801.0 12 2459.0 13 3188.0 14 3671.0 15 4630.0 16 5977.0 17 8324.0 18 7528.0 19 4422.0 20 4135.0 21 4459.0 22 4817.0 23 5212.0 24 5808.0 25 6732.0 26 7589.0 27 9091.0 28 10871.0 29 13122.0 30 15963.0 31 20470.0 32 26497.0 33 34915.0 34 49085.0 35 71357.0 36 108805.0 37 159993.0 38 148109.0 39 28057.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.5174052246263 18.967055488712607 15.026408630217999 21.489130656443095 2 14.89214189401026 20.63781954063127 40.979707943240356 23.490330622118112 3 19.968445253279093 25.31172419418149 30.31536904780111 24.404461504738308 4 12.265751577165423 16.847657738850213 39.078774776060996 31.80781590792337 5 13.067908642350027 37.68458425739708 34.60510861123262 14.642398489020273 6 29.856156861344502 36.65047150497654 18.280464145085517 15.212907488593444 7 26.768422340864934 33.633169647182406 21.681785963785078 17.916622048167582 8 25.45652550931924 33.426976321883636 20.648725299164482 20.467772869632636 9 25.732944707523377 17.349566876024795 21.750167665006593 35.16732075144524 10-11 24.275255139772224 26.513689058443713 27.90924368462123 21.301812117162836 12-13 26.157883026895668 24.375994905066058 28.14001123282484 21.32611083521344 14-15 22.80504923140866 25.50853756643977 27.885962642011226 23.800450560140344 16-17 20.907380425180406 28.87941748983705 28.198827994039267 22.014374090943278 18-19 20.65579559789922 27.74022136081507 30.707040186747292 20.89694285453842 20-21 22.17758256159413 25.944205310993528 30.431224220914686 21.446987906497654 22-23 21.32728784774553 26.083041520760382 30.840133442517438 21.749537188976653 24-25 21.778509464928288 26.170182672606245 30.662965473654165 21.388342388811303 26-27 21.27876449253896 25.95740424683392 31.418238158307993 21.345593102319125 28-29 20.702111379892294 27.130986336331265 30.910076661036918 21.256825622739527 30-31 21.126593665347478 26.19931042039892 30.814409818227222 21.859686096026383 32-33 21.111515308277497 26.37736909267788 30.87693300570003 21.63418259334459 34-35 21.970164658212365 26.265166935840956 30.41835099690051 21.34631740904617 36-37 21.605231253221273 25.95641174458141 31.169699097183244 21.26865790501407 38-39 21.174033976308575 26.56888465306986 30.930150183285534 21.326931187336033 40-41 21.288165093894857 26.759907443037456 30.626301263738924 21.32562619932876 42-43 20.951458722872914 26.803423595029606 29.899247943071742 22.345869739025733 44-45 21.26593922961664 26.46886817454412 29.987730233316682 22.277462362522552 46-47 22.014549082289143 26.88554208040792 29.677248377259396 21.422660460043534 48-49 21.716814260769187 26.926490046396424 29.476541551970943 21.880154140863446 50-51 21.007460618496804 27.289261895039147 30.00944761099032 21.69382987547373 52-53 21.49425477749208 27.734810008842725 28.812055156174743 21.95888005749045 54-55 21.1773475752527 27.081033038237194 29.261410153898105 22.480209232612 56-57 21.862676015958186 27.089733575772712 28.82609731638976 22.221493091879342 58-59 22.294814391979877 27.091546406464616 28.741338844533693 21.872300357021814 60-61 21.72587668304989 27.058059049687998 29.89243133220148 21.323632935060633 62-63 21.314011286908944 27.207783354548848 29.71764888768264 21.760556470859566 64-65 21.056319858316424 27.861144999506273 29.162353196003043 21.920181946174257 66-67 21.43311355161982 27.34714891209326 28.628628373504878 22.591109162782043 68-69 21.168111159335222 27.253016609275726 29.17524662808313 22.403625603305922 70-71 21.844491867897638 27.189446496911103 28.698901866597314 22.267159768593945 72-73 21.539744497158352 26.72070709548197 29.651032784201202 22.08851562315847 74-75 21.035187559094048 28.12398855200149 28.722001208003405 22.118822680901058 76-77 21.242663611577143 27.39366497961327 28.63840581418539 22.725265594624197 78-79 21.498983014558952 27.285795848456424 28.831218649320988 22.384002487663636 80-81 21.75744060710611 27.520779384708362 28.409266222200124 22.312513785985406 82-83 21.465261639299136 27.310224766570425 29.196714239379297 22.027799354751142 84-85 21.429472509212864 27.54507041535631 28.653800084610566 22.371656990820263 86-87 21.308792726267043 27.7130092278987 28.702709488466876 22.275488557367375 88-89 21.222299411730962 27.436536414681868 28.16801677490902 23.173147398678147 90-91 21.426950839146667 27.035410243108444 28.590065349813333 22.94757356793156 92-93 21.770774183145868 27.268770166828226 28.807551339972814 22.152904310053096 94-95 21.244389226039726 27.197719055128143 28.861725681336708 22.69616603749542 96-97 20.604415209075082 27.338093284162184 29.470832451939614 22.586659054823112 98-99 20.87445526583792 27.757511563850684 28.442611562958458 22.925421607352938 100-101 20.68785909006291 27.291864832953788 28.794400936407428 23.22587514057587 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 143.0 1 114.0 2 136.5 3 190.0 4 168.5 5 171.0 6 303.5 7 597.5 8 1005.5 9 1280.0 10 1215.0 11 1092.5 12 1097.0 13 1148.5 14 1229.0 15 1283.5 16 1327.5 17 1426.5 18 1533.5 19 1633.5 20 1802.5 21 2085.5 22 2443.5 23 2881.0 24 3436.0 25 4225.5 26 5289.0 27 6486.0 28 7581.5 29 8742.5 30 10287.0 31 12017.0 32 13673.0 33 15345.5 34 17209.0 35 18796.5 36 20576.5 37 22276.0 38 23048.5 39 23567.0 40 23978.0 41 24649.0 42 25205.0 43 25771.5 44 26883.0 45 27731.5 46 28293.5 47 32069.5 48 40621.0 49 39707.0 50 30427.0 51 25842.0 52 24018.0 53 23097.0 54 22077.5 55 20066.5 56 17784.5 57 15967.5 58 14423.0 59 12802.0 60 10710.5 61 8594.0 62 6807.0 63 5197.5 64 3775.0 65 2669.5 66 1958.0 67 1474.0 68 1013.0 69 699.5 70 501.0 71 343.5 72 249.5 73 214.5 74 150.5 75 97.5 76 72.5 77 41.5 78 24.0 79 15.0 80 9.0 81 8.0 82 5.5 83 3.5 84 3.0 85 6.5 86 5.5 87 1.0 88 2.5 89 3.0 90 0.5 91 0.0 92 0.0 93 0.0 94 1.5 95 2.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035165148789350564 2 0.028794650820265315 3 0.025481991876340992 4 0.04981729411824663 5 0.09441077990184336 6 0.1132674538903357 7 0.05606038212795018 8 0.08714841221708618 9 0.072114037010045 10-11 0.07542669595396934 12-13 0.07128587227406391 14-15 0.06510648924405123 16-17 0.051091393712063685 18-19 0.06274940499548969 20-21 0.05586926718887762 22-23 0.03319029441893414 24-25 0.009683156913009576 26-27 0.003121544004851771 28-29 0.006306792989394395 30-31 0.005351218294031608 32-33 0.006051973070630985 34-35 0.0077083025425931494 36-37 0.0059245631112492805 38-39 0.0034400689033060334 40-41 0.006880137806612067 42-43 0.0026119041673249516 44-45 0.0015289195125804595 46-47 9.555746953627871E-4 48-49 1.9111493907255744E-4 50-51 0.0017200344516530167 52-53 0.005351218294031608 54-55 0.012167651120952824 56-57 0.00853646727857423 58-59 0.005351218294031608 60-61 0.0026119041673249516 62-63 0.008281647359810821 64-65 0.0026119041673249516 66-67 0.019111493907255743 68-69 0.042873451331943715 70-71 0.056824841884240404 72-73 0.0029941340454700664 74-75 0.012995815856933907 76-77 0.021213758237053876 78-79 0.024972352038814167 80-81 0.07096734737560965 82-83 0.02669238649046719 84-85 0.012231356100643676 86-87 0.03013245539377322 88-89 0.0462498152555589 90-91 0.04089859696152729 92-93 0.04058007206307303 94-95 0.01344175071476987 96-97 0.016053654882094825 98-99 0.03962449736771024 100-101 0.07568151587273274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 784868.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.2507604465379 #Duplication Level Percentage of deduplicated Percentage of total 1 86.54558051337173 60.798928443514356 2 8.570466855908515 12.041636280188438 3 2.1555575580244497 4.542886729124994 4 0.892265074977334 2.5072920014817943 5 0.4679096401532011 1.643550402051414 6 0.3010584866175984 1.268975257426208 7 0.19548483370524883 0.9613070756491112 8 0.1417489206059864 0.7966375572037174 9 0.1004368468080794 0.6350188378608009 >10 0.5852625821775189 7.197843949250443 >50 0.029668307248060053 1.4196864361461026 >100 0.010374271051682303 1.3758984938429881 >500 0.0020020522981389665 0.9879720873339594 >1k 0.002184057052515236 3.8223664489256777 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4527 0.5767848861209783 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4366 0.5562718826605237 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3930 0.5007211403701004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3403 0.433576091775942 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2575 0.3280806454078902 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2256 0.2874368683651264 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2037 0.25953408726053295 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1628 0.20742341387341565 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1606 0.20462039476701813 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1280 0.16308474800858233 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1239 0.15786093967393242 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1048 0.13352563743202678 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 933 0.11887349210313071 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.11161112441837355 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 834 0.10625990612434191 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2740995938170495E-4 0.0 24-25 0.0 0.0 0.0 7.007547765993772E-4 0.0 26-27 0.0 0.0 0.0 0.0014015095531987544 0.0 28-29 0.0 0.0 0.0 0.0037585938017602964 0.0 30-31 0.0 0.0 0.0 0.007326072664448034 0.0 32-33 0.0 0.0 0.0 0.009555746953627871 0.0 34-35 0.0 0.0 0.0 0.013505455694460725 0.0 36-37 0.0 0.0 0.0 0.017136639536839318 0.0 38-39 0.0 0.0 0.0 0.020194478562000233 0.0 40-41 0.0 0.0 0.0 0.024526417180978203 0.0 42-43 0.0 0.0 0.0 0.03115173506882686 0.0 44-45 0.0 0.0 0.0 0.04096230194121814 0.0 46-47 0.0 0.0 0.0 0.0694384278630292 0.0 48-49 0.0 0.0 0.0 0.12690031954417813 0.0 50-51 0.0 0.0 0.0 0.16053654882094825 0.0 52-53 0.0 0.0 0.0 0.18882155980368673 0.0 54-55 0.0 0.0 0.0 0.23048461652150426 0.0 56-57 0.0 0.0 0.0 0.2642482557576561 0.0 58-59 0.0 0.0 0.0 0.3024075385924767 0.0 60-61 0.0 0.0 0.0 0.3789172192011905 0.0 62-63 0.0 0.0 0.0 0.42898933323820054 0.0 64-65 0.0 0.0 0.0 0.4879164394522391 0.0 66-67 0.0 0.0 0.0 0.5976801194595779 0.0 68-69 0.0 0.0 0.0 0.7223507647145762 0.0 70-71 0.0 0.0 0.0 0.8231320425855049 0.0 72-73 0.0 0.0 0.0 0.9399669753385282 0.0 74-75 0.0 0.0 0.0 1.0353333299357343 0.0 76-77 0.0 0.0 0.0 1.1029880183674199 0.0 78-79 0.0 0.0 0.0 1.1929394496909036 0.0 80-81 0.0 0.0 0.0 1.3258280373260218 0.0 82-83 0.0 0.0 0.0 1.4791022184622127 0.0 84-85 0.0 0.0 0.0 1.6514878935056596 0.0 86-87 0.0 0.0 0.0 2.00575128556649 0.0 88-89 0.0 0.0 0.0 2.4732821315176565 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3820 0.0 27.38038 1 TACATGG 3795 0.0 27.184923 2 TACACCG 70 0.008353622 27.167028 5 ACATGGG 3890 0.0 25.298857 3 GAGTACT 3405 0.0 24.918285 12-13 GTACTTT 3715 0.0 22.6456 14-15 CATGGGA 2400 0.0 22.186405 4 AGTACTT 3650 0.0 21.943409 12-13 GTGTAGC 425 0.0 21.254204 1 AGAGTAC 5810 0.0 20.73556 10-11 CATGGGG 1395 0.0 20.448301 4 ATGGGAT 1120 0.0 20.37527 5 GCACCGT 170 7.907173E-5 19.572496 6 ACTTTTT 4455 0.0 19.255611 16-17 GTATCAA 9650 0.0 19.01692 1 GCTACAC 375 9.094947E-12 19.01692 3 TACTTTT 4295 0.0 18.702208 14-15 ATGGGAG 1020 0.0 18.177938 5 GTATAGA 210 1.9395975E-5 18.111353 1 TACCTGG 635 0.0 17.220045 2 >>END_MODULE