FastQCFastQC Report
Thu 26 May 2016
SRR936920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784868
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71880.9158227880356952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55560.7078897343247527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.5981897592971047No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.3646473037504396No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24600.31342850007899414No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18340.23366986550604688No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18300.23316022566852007No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16660.21226499232992044No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16240.20691377403588884No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15230.19404536813833664No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC15200.19366313826019152No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14740.1878022801286331No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13830.17620797382489795No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.16117359861785677No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10310.1313596681225378No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG700.00838783827.1442782
TAACGCA908.9456025E-426.388594
GAGTACT49250.022.18460712-13
GTACATG39250.022.1575371
TACATGG39050.021.7745082
GTACTTT50800.021.31865514-15
ACATGGG37800.021.236533
AGTACTT51500.020.70805412-13
AGAGTAC74250.020.28181510-11
ACTATCC2351.12933776E-720.2151098
TACACGA1200.004760736619.7927022
ACCGTGG1507.712053E-419.0022058
AGTACAT30400.018.750982
GCTACAC3305.966285E-1018.7119083
GTAAGGT2803.484456E-818.6605014
GTGTAGC5100.018.6367381
ACTTTTT58850.018.4440416-17
TACTTTT56400.018.4439414-15
GTATAGG2603.2596836E-718.278341
TAAGGTG3901.4551915E-1118.2690245
Produced by FastQC (version 0.11.5)