##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784868 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3569899651916 33.0 31.0 34.0 31.0 34.0 2 32.47927549600697 34.0 31.0 34.0 31.0 34.0 3 32.59564156010947 34.0 31.0 34.0 31.0 34.0 4 36.21018056539444 37.0 35.0 37.0 35.0 37.0 5 36.090566821427295 37.0 35.0 37.0 35.0 37.0 6 36.196602486023124 37.0 36.0 37.0 35.0 37.0 7 36.17448411707446 37.0 36.0 37.0 35.0 37.0 8 36.13339695337305 37.0 36.0 37.0 35.0 37.0 9 37.86129897001789 39.0 38.0 39.0 35.0 39.0 10-11 37.782399078571174 39.0 37.0 39.0 35.0 39.0 12-13 37.69344972657822 39.0 37.0 39.0 35.0 39.0 14-15 38.89995897399308 40.0 38.0 41.0 36.0 41.0 16-17 38.91627764159069 40.0 38.0 41.0 35.5 41.0 18-19 39.07396785191905 40.0 38.5 41.0 36.0 41.0 20-21 39.08838110357411 40.0 38.5 41.0 36.0 41.0 22-23 38.998982631474334 40.0 38.0 41.0 35.5 41.0 24-25 38.89604239184169 40.0 38.0 41.0 35.0 41.0 26-27 38.68985039522569 40.0 38.0 41.0 35.0 41.0 28-29 38.55168385002319 40.0 38.0 41.0 34.0 41.0 30-31 38.52465255304077 40.0 38.0 41.0 35.0 41.0 32-33 38.34704115851328 40.0 38.0 41.0 34.0 41.0 34-35 38.188288986173475 40.0 38.0 41.0 34.0 41.0 36-37 38.028456377378106 40.0 37.5 41.0 34.0 41.0 38-39 37.846467813696066 40.0 37.0 41.0 33.0 41.0 40-41 37.48564344577687 40.0 37.0 41.0 33.0 41.0 42-43 37.28390824954005 40.0 37.0 41.0 33.0 41.0 44-45 37.01144842190024 40.0 36.5 41.0 32.0 41.0 46-47 36.54085909987412 39.5 36.0 41.0 31.0 41.0 48-49 36.06281438407477 39.0 36.0 41.0 30.0 41.0 50-51 35.7426439859951 39.0 35.0 40.0 28.5 41.0 52-53 35.50929837883568 39.0 35.0 40.0 28.0 41.0 54-55 35.13806206903581 38.5 35.0 40.0 27.0 41.0 56-57 34.91414543592043 38.0 34.5 40.0 26.5 41.0 58-59 34.606535748686404 38.0 34.0 40.0 26.0 41.0 60-61 34.1879940321175 38.0 34.0 40.0 25.0 41.0 62-63 33.84053751713664 37.0 33.5 40.0 24.5 41.0 64-65 33.54989565124327 37.0 33.0 39.0 23.0 40.0 66-67 33.207661033447664 36.0 33.0 39.0 22.5 40.0 68-69 32.741885896736775 36.0 32.0 39.0 21.5 40.0 70-71 32.271741235468895 35.0 32.0 38.0 20.0 40.0 72-73 31.844387846109157 35.0 31.0 37.5 18.5 39.5 74-75 31.388665355193485 35.0 31.0 37.0 18.0 39.0 76-77 29.9902939092943 33.5 29.5 35.5 15.5 38.0 78-79 30.63658729875597 34.0 31.0 36.0 14.0 38.0 80-81 30.46135847046892 34.0 31.0 35.5 9.5 37.0 82-83 30.10175086766182 34.0 30.0 35.0 7.0 37.0 84-85 29.669969982213573 34.0 30.0 35.0 4.5 36.5 86-87 29.33666743962042 34.0 29.5 35.0 2.0 36.0 88-89 28.965016410402768 34.0 29.0 35.0 2.0 36.0 90-91 28.49963114816759 34.0 29.0 35.0 2.0 35.0 92-93 28.034661879449793 33.0 28.5 35.0 2.0 35.0 94-95 27.48815533312608 33.0 27.0 35.0 2.0 35.0 96-97 26.741747656930848 33.0 25.5 34.0 2.0 35.0 98-99 25.967944928319156 32.0 24.5 34.0 2.0 35.0 100-101 22.839359612062154 28.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.044846251491840405 1101 2 0.0967137587517044 1101 3 0.12112728748402901 1101 4 0.022092207150599563 1101 5 0.0052565436017602 1101 6 0.06976212601259135 1101 7 0.006580628500245211 1101 8 0.14422376990154362 1101 9 0.13330972334340885 1101 10-11 0.0828852026621334 1101 12-13 0.12937342677290076 1101 14-15 0.05187340826788045 1101 16-17 0.09129718271778842 1101 18-19 0.1045861173374405 1101 20-21 0.01956817789177734 1101 22-23 0.02025298785710561 1101 24-25 -0.10836980398849505 1101 26-27 -0.14187826165632345 1101 28-29 0.01777584061417059 1101 30-31 0.11820794576551208 1101 32-33 0.13757373789564298 1101 34-35 -0.043356420323455325 1101 36-37 -0.13575160292598554 1101 38-39 -0.09117934052775212 1101 40-41 0.10069584934607434 1101 42-43 0.14082806752865196 1101 44-45 -0.02853931669284293 1101 46-47 -0.01762306928558388 1101 48-49 0.25916633807862866 1101 50-51 0.07695994262378747 1101 52-53 0.13552891417955237 1101 54-55 -0.060451940932942705 1101 56-57 0.1679665929160663 1101 58-59 0.013928875442047683 1101 60-61 0.006581883460285098 1101 62-63 -0.09758684701724718 1101 64-65 0.098526915852581 1101 66-67 -0.005038454972847717 1101 68-69 -0.18168814524582189 1101 70-71 -0.40937032382142036 1101 72-73 -0.30041855729797007 1101 74-75 -0.3413852611766188 1101 76-77 -0.01567003771862474 1101 78-79 0.01807924158772778 1101 80-81 -0.2791553120226311 1101 82-83 -0.15433341621127283 1101 84-85 -0.23013269052723118 1101 86-87 -0.5497252584407946 1101 88-89 -0.40924452923958654 1101 90-91 -0.1553730855961426 1101 92-93 -0.6153559563548932 1101 94-95 -0.7565589607593282 1101 96-97 -0.8905000543573784 1101 98-99 -0.5325289044354342 1101 100-101 -0.36864762314971244 1102 1 -0.1961273408238995 1102 2 -0.07880564177256133 1102 3 0.001361816619549927 1102 4 -0.06975114368905366 1102 5 -0.1981965552024363 1102 6 -0.09960458974587993 1102 7 -0.028712208728009614 1102 8 0.037671403769330425 1102 9 0.1900570672916828 1102 10-11 0.06585706287091142 1102 12-13 0.13757662051879294 1102 14-15 -0.026348125434104475 1102 16-17 0.0027015324366672644 1102 18-19 0.09436136024923059 1102 20-21 0.086650272351811 1102 22-23 -0.10625797443483975 1102 24-25 -0.08288953796650844 1102 26-27 0.04823437160938937 1102 28-29 -0.10003174731109965 1102 30-31 -0.04510218474458583 1102 32-33 -0.0559139567348339 1102 34-35 0.04400730361388838 1102 36-37 0.08243945680854381 1102 38-39 0.15142562793308656 1102 40-41 0.17970123091904355 1102 42-43 0.2214231435283196 1102 44-45 0.18273141996206022 1102 46-47 -0.113612593280358 1102 48-49 0.10052571785465858 1102 50-51 0.10547371148475548 1102 52-53 0.094725985344148 1102 54-55 0.5641347153596072 1102 56-57 0.37216648917490147 1102 58-59 0.007119703611479622 1102 60-61 0.04910534100513786 1102 62-63 0.16318093023564728 1102 64-65 0.21120186328730028 1102 66-67 0.12043278001016233 1102 68-69 -0.06411212242277031 1102 70-71 -0.1452749560497253 1102 72-73 -0.16020704573361044 1102 74-75 -0.202406833781545 1102 76-77 0.03385942199367875 1102 78-79 -0.26172087141604194 1102 80-81 0.023386809669460717 1102 82-83 0.217379517162815 1102 84-85 0.16985202617602368 1102 86-87 0.03637527786398209 1102 88-89 -0.09751390429169149 1102 90-91 -0.010621740202896746 1102 92-93 -0.05322935508220894 1102 94-95 -0.3133456707749822 1102 96-97 0.330241694978028 1102 98-99 0.01934052063472791 1102 100-101 0.11187890957970126 1103 1 -0.0019468472876766896 1103 2 0.06493648816376663 1103 3 0.008257130233687349 1103 4 0.06122104411700491 1103 5 -0.10285226151484039 1103 6 -0.10197214089392048 1103 7 0.024173776279468484 1103 8 -0.13461029520357926 1103 9 -0.117662211435416 1103 10-11 -0.10546463583229126 1103 12-13 0.09339975121537236 1103 14-15 0.04884880526213209 1103 16-17 0.054702951787369614 1103 18-19 -0.0017166298692501414 1103 20-21 -0.14694375288970463 1103 22-23 -0.08583457975925057 1103 24-25 -0.0528252626192085 1103 26-27 -0.03390001494285144 1103 28-29 -0.11716594000903058 1103 30-31 -0.05809505933432746 1103 32-33 -0.08563519423333332 1103 34-35 -0.24592584193361233 1103 36-37 -0.25172534295681004 1103 38-39 -0.4132483849691866 1103 40-41 -0.23941684995697443 1103 42-43 -0.38564385757917563 1103 44-45 -0.36147452748824804 1103 46-47 -0.4567111268989166 1103 48-49 -0.33204079524568186 1103 50-51 -0.28993092989318825 1103 52-53 -0.5832942227036781 1103 54-55 -0.492253711369365 1103 56-57 -0.5563206549127955 1103 58-59 -0.7036741045132047 1103 60-61 -0.6843517219503141 1103 62-63 -0.5153850198443379 1103 64-65 -0.7922521148560477 1103 66-67 -0.906389492670371 1103 68-69 -0.7697949194539291 1103 70-71 -0.8807506323905656 1103 72-73 -0.6085903850511407 1103 74-75 -0.7510726946885455 1103 76-77 -0.5977364709920003 1103 78-79 -0.5792319000407353 1103 80-81 -0.6985814353084479 1103 82-83 -0.39505893409294046 1103 84-85 -0.8216525192861823 1103 86-87 -0.9383444340853657 1103 88-89 -0.5397175052619723 1103 90-91 -0.9803168326545482 1103 92-93 -0.7933183426401307 1103 94-95 -0.6939086574125213 1103 96-97 -0.28061178656114194 1103 98-99 -0.5747500413573512 1103 100-101 0.13754100634165667 1104 1 0.07887297349643774 1104 2 0.18325047746413503 1104 3 0.01660296801847494 1104 4 -0.021325501726032314 1104 5 -0.043186337373605 1104 6 0.07211102868664199 1104 7 0.16715771874619634 1104 8 0.13860304925081124 1104 9 0.17800360768861623 1104 10-11 0.14408752641120515 1104 12-13 0.17631587680786254 1104 14-15 0.3093348733173187 1104 16-17 0.29257548514548404 1104 18-19 0.27606849686832646 1104 20-21 0.2151328208030847 1104 22-23 0.287838315908175 1104 24-25 0.12764174077567247 1104 26-27 0.2564503183857667 1104 28-29 0.2641262562034825 1104 30-31 0.29003341304917996 1104 32-33 0.38359678673431574 1104 34-35 0.3701267434080009 1104 36-37 0.2831146168943093 1104 38-39 0.29393943626730845 1104 40-41 0.4026704072160072 1104 42-43 0.40551379544804433 1104 44-45 0.5465238447452023 1104 46-47 0.23893490052269328 1104 48-49 0.22655173197296108 1104 50-51 0.37921239232582593 1104 52-53 0.12018012987540772 1104 54-55 0.20184280751252714 1104 56-57 0.26503499592118374 1104 58-59 -0.10655642264664777 1104 60-61 0.06382713849193067 1104 62-63 0.15425713405836206 1104 64-65 -0.009533704331708748 1104 66-67 -0.35589793140798776 1104 68-69 -0.32691922548212204 1104 70-71 -0.04838837153189246 1104 72-73 0.03452987781498251 1104 74-75 0.03692585930544112 1104 76-77 0.1360570530821832 1104 78-79 0.19075872919403025 1104 80-81 0.08608888137898774 1104 82-83 0.28253473821705555 1104 84-85 0.1598397144901753 1104 86-87 0.2366152251742193 1104 88-89 0.36179756975463917 1104 90-91 0.39630462276262435 1104 92-93 0.18790125033745042 1104 94-95 0.41560913189471194 1104 96-97 0.20936714941270296 1104 98-99 0.08674772601744252 1104 100-101 0.7743612471945625 1105 1 -0.1287646011636312 1105 2 0.022728248808398632 1105 3 -0.03942948798211887 1105 4 0.039392193504141915 1105 5 0.07355086047250126 1105 6 0.02417055207980212 1105 7 0.07027546889872838 1105 8 0.09433356185510178 1105 9 0.14532480888188104 1105 10-11 0.009571160457362282 1105 12-13 0.06891806929775157 1105 14-15 -0.0023293904964987178 1105 16-17 -0.08261606303010183 1105 18-19 0.01853124617240809 1105 20-21 -0.015115534440901968 1105 22-23 -0.15544205103530118 1105 24-25 -0.06629374359671658 1105 26-27 -0.06708637013326069 1105 28-29 -0.041776989785176966 1105 30-31 0.02095987797983412 1105 32-33 0.014968070894006757 1105 34-35 0.008011637041285269 1105 36-37 0.015269710359454791 1105 38-39 -0.11470552311735815 1105 40-41 -0.07262036388603121 1105 42-43 0.027920689757493733 1105 44-45 0.06511259939384928 1105 46-47 -0.14785834661881836 1105 48-49 -0.392489225333982 1105 50-51 -0.41198487606442313 1105 52-53 -0.17376649440778635 1105 54-55 -0.050378374121130776 1105 56-57 -0.1893302299405093 1105 58-59 -0.0695796908115014 1105 60-61 -0.16652808217545356 1105 62-63 -0.10951198999490686 1105 64-65 -0.2865016796788282 1105 66-67 -0.34052420683062223 1105 68-69 -0.23324229066744095 1105 70-71 -0.311463194167672 1105 72-73 -0.39950889684664403 1105 74-75 -0.20934648042556958 1105 76-77 -0.3454273788924702 1105 78-79 -0.1092546127921672 1105 80-81 0.002076466855136516 1105 82-83 -0.02588923642539598 1105 84-85 -0.5204765812563252 1105 86-87 -0.429096777043398 1105 88-89 -0.21945642150700806 1105 90-91 -0.21359383565506818 1105 92-93 -0.22604423404789387 1105 94-95 -0.013803989106740744 1105 96-97 -0.1276630799010654 1105 98-99 -0.24456228863283513 1105 100-101 0.18123483122769812 1106 1 -0.06026958932402948 1106 2 0.055961624841934565 1106 3 0.07377943247904284 1106 4 0.10107458161019167 1106 5 0.039565378542455676 1106 6 0.004082046198817579 1106 7 0.05067219262839728 1106 8 0.1165522257268421 1106 9 0.008101874989563385 1106 10-11 0.01838293018679593 1106 12-13 0.02569866787699482 1106 14-15 0.18806257798298986 1106 16-17 0.18408068787243081 1106 18-19 0.1739866826849621 1106 20-21 0.16039929417599552 1106 22-23 0.03634737872286564 1106 24-25 0.046740494204613015 1106 26-27 0.13234299984461728 1106 28-29 0.18157419999782576 1106 30-31 0.22791030692280856 1106 32-33 0.1663330217887804 1106 34-35 0.28946422040373676 1106 36-37 0.19857247422731916 1106 38-39 0.0603628942577501 1106 40-41 0.15209674725095823 1106 42-43 0.032615286240549324 1106 44-45 -0.2197390173912126 1106 46-47 -0.26225365347801954 1106 48-49 -0.37357254896271996 1106 50-51 -0.39062264245548306 1106 52-53 -0.4551899639935968 1106 54-55 -0.4062700320536621 1106 56-57 -0.46427345807605747 1106 58-59 -0.3526116965566217 1106 60-61 -0.29757646927222936 1106 62-63 -0.23347117248005844 1106 64-65 -0.24903710144040048 1106 66-67 -0.12921398983594656 1106 68-69 -0.11067329101680201 1106 70-71 -0.09000439986620279 1106 72-73 -0.11183081757953417 1106 74-75 -0.25436608370183933 1106 76-77 -0.23916937260389304 1106 78-79 -0.11719539537048007 1106 80-81 0.09522114291584671 1106 82-83 0.24401488220353684 1106 84-85 0.35218218587228023 1106 86-87 0.11347726899518662 1106 88-89 0.5627167278272545 1106 90-91 0.3468924614607296 1106 92-93 0.5643220172622243 1106 94-95 0.8574112199301744 1106 96-97 0.8261333565726723 1106 98-99 0.9563858500263791 1106 100-101 1.0961012960388459 1107 1 0.15091641613256002 1107 2 0.20984141941744383 1107 3 0.26011790142463553 1107 4 0.09881170245756721 1107 5 -0.04379202797335324 1107 6 0.10366477770259053 1107 7 0.19979753366514785 1107 8 0.18730732121713345 1107 9 0.13207876473438773 1107 10-11 0.11343990338531995 1107 12-13 0.16947575020728323 1107 14-15 0.27423454074980214 1107 16-17 0.19017521523560532 1107 18-19 0.1396081720125224 1107 20-21 0.1372067889601425 1107 22-23 0.10089359923321695 1107 24-25 0.3126167580466941 1107 26-27 0.21873362735224333 1107 28-29 0.15862443270525972 1107 30-31 0.2610671035846579 1107 32-33 0.1547879246936006 1107 34-35 0.16517437928673928 1107 36-37 0.32347417539887857 1107 38-39 0.23753148677901237 1107 40-41 0.5501367889778095 1107 42-43 0.5050494862084562 1107 44-45 0.32168892354924594 1107 46-47 0.45634087388516065 1107 48-49 0.3359704617899695 1107 50-51 0.33151064273148734 1107 52-53 0.2558435779739838 1107 54-55 0.2815288766287338 1107 56-57 0.25854591281143513 1107 58-59 -0.06072235295206241 1107 60-61 -0.14339375381724295 1107 62-63 0.08596190407265425 1107 64-65 -0.04843928407561293 1107 66-67 0.08889907387774798 1107 68-69 0.011650573531419184 1107 70-71 -0.08935442393939041 1107 72-73 0.23326226539270678 1107 74-75 -0.098163227017519 1107 76-77 -0.4032501782530673 1107 78-79 -0.0927711456513336 1107 80-81 -0.08060938187953326 1107 82-83 -0.06630448868396144 1107 84-85 -0.19337831805967198 1107 86-87 0.07279359061290336 1107 88-89 0.29833099520007167 1107 90-91 0.06799461199836188 1107 92-93 0.22787120679841522 1107 94-95 0.1773719003987999 1107 96-97 0.20044727087214298 1107 98-99 0.2793075319393914 1107 100-101 0.3246962246217393 1108 1 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-0.5924605277668178 2316 70-71 -0.5915314088373833 2316 72-73 -0.486242282871892 2316 74-75 -0.39416693628184873 2316 76-77 -0.3829507876322076 2316 78-79 -0.37299723414378505 2316 80-81 -0.5547836289871881 2316 82-83 -0.5076473306869644 2316 84-85 -0.4084074622794027 2316 86-87 -0.24100354795461243 2316 88-89 -0.7360481446038705 2316 90-91 -0.8854077548655432 2316 92-93 -0.8909334386135761 2316 94-95 -0.8393661548869034 2316 96-97 -0.4695098959564348 2316 98-99 -1.1387022711163368 2316 100-101 -2.065825280279789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 6.0 5 10.0 6 35.0 7 79.0 8 181.0 9 350.0 10 618.0 11 1124.0 12 1861.0 13 2716.0 14 3405.0 15 4962.0 16 6922.0 17 10593.0 18 9714.0 19 5192.0 20 4042.0 21 3976.0 22 3955.0 23 4294.0 24 4932.0 25 5550.0 26 6764.0 27 8170.0 28 10226.0 29 13158.0 30 16537.0 31 21067.0 32 27311.0 33 37645.0 34 53496.0 35 79380.0 36 126092.0 37 184603.0 38 119180.0 39 6719.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69585075513224 19.569591829712614 15.280930091487544 20.453627323667607 2 15.531748984511573 21.947291436259945 39.722033932858 22.79892564637049 3 20.88552921270437 25.78548640273984 30.184465079300367 23.144519305255432 4 12.941411804277916 17.724126859548356 38.413593113746515 30.92086822242721 5 13.150078932581764 38.32173659324683 33.9165467919225 14.611637682248896 6 29.996891196991086 37.82355759184984 17.215761121615355 14.96379008954372 7 26.9807496047101 34.49629045430627 20.870287577385724 17.652672363597908 8 26.076486746816023 33.8584322459318 19.670059169185137 20.395021838067038 9 26.272061034466944 17.918172227686693 20.8765040745705 34.93326266327586 10-11 24.751677989165056 27.308859578935568 26.86610997008414 21.073352461815233 12-13 27.065165315161494 24.995349157241808 27.349248984459813 20.590236543136886 14-15 23.089743009840916 26.125920654710445 27.705551035854008 23.078785299594628 16-17 20.97187042916771 29.310087810944008 27.905329303781016 21.81271245610727 18-19 20.644352029518416 28.003124100164868 31.0915493855002 20.260974484816515 20-21 22.312931843982565 25.95293943768387 31.081427157165407 20.652701561168154 22-23 21.533080845886037 26.260626929092222 31.13163854585068 21.07465367917106 24-25 22.10356055089764 26.170377179755235 31.334835503453146 20.391226765893986 26-27 21.677571918281643 25.94345662606316 31.96753842850982 20.41143302714538 28-29 20.775927146938812 27.426030508334208 31.461616132659337 20.336426212067643 30-31 21.386016502137938 26.352138193938345 31.206776171279753 21.05506913264396 32-33 21.41183515636447 26.63534493193097 31.15338306587757 20.79943684582699 34-35 22.014338716828817 26.33697640877192 31.146766080410977 20.50191879398829 36-37 21.921069446629918 26.011636551743027 31.609298283949443 20.45799571767761 38-39 21.27105561760278 26.799813709668683 31.563787555117788 20.365343117610742 40-41 21.428362111142825 26.870706262486237 30.890128735679045 20.81080289069189 42-43 21.23337937079866 27.27687967913076 30.15468843168533 21.335052518385258 44-45 21.868721037939103 26.389637427168815 30.25528022087931 21.486361314012765 46-47 22.533258840834716 27.263617443221232 29.59942869265028 20.60369502329377 48-49 22.41706073580666 27.364462618802047 29.30014212608131 20.918334519309973 50-51 21.48313123604038 27.79176482729887 29.86922391836433 20.855880018296418 52-53 22.17400444662458 28.49126919916164 28.323023704713552 21.011702649500233 54-55 21.56719346437872 27.77097550161301 28.80994001539112 21.851891018617142 56-57 22.335928894823383 27.761317066427303 28.629746115735756 21.273007923013562 58-59 22.967620032922735 27.435696193500053 28.420893704419086 21.175790069158126 60-61 21.942034931426562 27.746505073568013 29.73764480437076 20.57381519063467 62-63 21.643639440007746 27.74988915333534 29.469796195028973 21.136675211627942 64-65 21.428967309768847 28.659359700841225 28.860986090008822 21.050686899381105 66-67 21.87234031709791 27.81308449318866 28.393691678091095 21.920883511622336 68-69 21.63707782709959 27.950878364259978 28.651059796042137 21.760984012598296 70-71 22.45903592276119 27.783267048006376 28.47147501256586 21.28622201666657 72-73 22.239684531734756 27.335083103463653 29.101374121664236 21.323858243137355 74-75 21.59477857022269 28.873563804202757 28.31689160564062 21.214766019933936 76-77 21.976243138973686 28.012481079621033 28.042040190197586 21.969235591207696 78-79 22.054953434637444 28.165880862238 28.01547273446563 21.763692968658923 80-81 22.458720108351155 28.170918086195485 27.948265636158503 21.422096169294857 82-83 22.23051803681388 27.96868768848735 28.404494009813124 21.396300264885642 84-85 21.91391863800813 28.351386665358564 28.223527920285456 21.51116677634785 86-87 21.80338134799064 28.559388276575127 27.814355216728494 21.822875158705738 88-89 21.64029108802527 28.550095973936372 27.34897244500053 22.46064049303783 90-91 22.034913277416706 27.79703105908195 27.729311889141872 22.43874377435947 92-93 22.434982697727516 28.219140033738157 27.74058822630047 21.605289042233853 94-95 22.047274191328988 28.118295050887536 27.84723036230296 21.987200395480514 96-97 21.31908035139801 28.190262019582985 28.696591133507038 21.79406649551197 98-99 21.61280787298643 28.713300826515777 27.370133055407265 22.30375824509053 100-101 21.185354973890032 28.800788937043663 27.21654423405132 22.797311855014982 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 356.0 1 275.5 2 222.5 3 259.0 4 235.5 5 215.0 6 353.0 7 753.0 8 1302.5 9 1593.5 10 1511.5 11 1352.0 12 1347.5 13 1448.0 14 1568.0 15 1683.5 16 1792.0 17 1925.5 18 2051.0 19 2126.0 20 2211.5 21 2446.5 22 2804.5 23 3271.0 24 3814.5 25 4588.0 26 5491.5 27 6513.0 28 7688.5 29 8947.0 30 10435.0 31 12120.0 32 13740.0 33 15328.0 34 16959.0 35 18242.0 36 20122.5 37 21742.5 38 22557.0 39 23068.5 40 23342.5 41 24257.5 42 24757.5 43 25097.5 44 26177.0 45 26904.5 46 27490.0 47 32195.5 48 41420.5 49 39559.5 50 29674.0 51 25422.5 52 23650.5 53 22747.5 54 21604.5 55 19797.0 56 17776.0 57 15910.0 58 14514.5 59 12955.0 60 10823.5 61 8694.5 62 6859.5 63 5294.0 64 3844.5 65 2733.5 66 1996.5 67 1543.0 68 1097.5 69 708.0 70 517.5 71 346.0 72 238.0 73 190.0 74 147.0 75 118.0 76 80.5 77 37.0 78 22.0 79 15.0 80 10.0 81 6.0 82 1.5 83 2.5 84 1.0 85 1.5 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021787103054271544 2 0.003057839025160919 3 5.096398375268198E-4 4 0.0 5 0.002675609147015804 6 0.0 7 1.2740995938170495E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.008154237400429117 14-15 0.004140823679905411 16-17 0.0 18-19 2.548199187634099E-4 20-21 8.918697156719347E-4 22-23 0.001337804573507902 24-25 4.4593485783596734E-4 26-27 1.9111493907255744E-4 28-29 6.370497969085248E-5 30-31 0.0 32-33 3.822298781451149E-4 34-35 0.0 36-37 0.0017200344516530167 38-39 0.009109812095791904 40-41 5.096398375268198E-4 42-43 0.0 44-45 6.370497969085247E-4 46-47 1.9111493907255744E-4 48-49 1.9111493907255744E-4 50-51 0.0019111493907255743 52-53 3.822298781451149E-4 54-55 0.0 56-57 3.1852489845426237E-4 58-59 0.0 60-61 0.0020385593501072792 62-63 0.0 64-65 6.370497969085248E-5 66-67 0.0 68-69 0.0 70-71 4.4593485783596734E-4 72-73 3.822298781451149E-4 74-75 0.0032489539642334767 76-77 0.0 78-79 4.4593485783596734E-4 80-81 0.001592624492271312 82-83 1.2740995938170495E-4 84-85 0.0022296742891798364 86-87 4.4593485783596734E-4 88-89 0.0024844942079432462 90-91 0.0011466896344353445 92-93 0.0 94-95 0.0 96-97 3.822298781451149E-4 98-99 8.918697156719347E-4 100-101 0.0018474444110347218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 784868.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.26821371581818 #Duplication Level Percentage of deduplicated Percentage of total 1 85.34514365375388 57.4101536290792 2 9.190028085968631 12.363935466826184 3 2.386962376065021 4.8170008573427685 4 1.0030082821892132 2.6988230194015865 5 0.5404476651860148 1.8177474521973893 6 0.34681812980510196 1.399790164574998 7 0.22589706103595422 1.063698424567925 8 0.15494421017792892 0.8338256195422058 9 0.11547033357309638 0.6990734768968675 >10 0.6358297115019947 7.599261160383103 >50 0.031549915230223 1.445344864574506 >100 0.020076483441472424 2.5738132511702 >500 9.560230178567831E-4 0.40142277457973763 >1k 0.0024856598464276358 3.236977560989509 >5k 3.8240920714271325E-4 1.639132277873815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7188 0.9158227880356952 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5556 0.7078897343247527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4695 0.5981897592971047 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2862 0.3646473037504396 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2460 0.31342850007899414 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1834 0.23366986550604688 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1830 0.23316022566852007 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1666 0.21226499232992044 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1624 0.20691377403588884 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1523 0.19404536813833664 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1520 0.19366313826019152 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1474 0.1878022801286331 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1383 0.17620797382489795 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1265 0.16117359861785677 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1031 0.1313596681225378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.2740995938170495E-4 0.0 24-25 0.0 0.0 0.0 5.733448172176723E-4 0.0 26-27 0.0 0.0 0.0 0.0014015095531987544 0.0 28-29 0.0 0.0 0.0 0.0037585938017602964 0.0 30-31 0.0 0.0 0.0 0.007644597562902297 0.0 32-33 0.0 0.0 0.0 0.010192796750536396 0.0 34-35 0.0 0.0 0.0 0.014015095531987544 0.0 36-37 0.0 0.0 0.0 0.01783739431343869 0.0 38-39 0.0 0.0 0.0 0.020767823379217905 0.0 40-41 0.0 0.0 0.0 0.024653827140359907 0.0 42-43 0.0 0.0 0.0 0.03166137490635368 0.0 44-45 0.0 0.0 0.0 0.04198158161627178 0.0 46-47 0.0 0.0 0.0 0.07033029757870113 0.0 48-49 0.0 0.0 0.0 0.12823812411768604 0.0 50-51 0.0 0.0 0.0 0.16181064841476528 0.0 52-53 0.0 0.0 0.0 0.18990454445843122 0.0 54-55 0.0 0.0 0.0 0.2321409459934664 0.0 56-57 0.0 0.0 0.0 0.2662231101280725 0.0 58-59 0.0 0.0 0.0 0.3049557377801108 0.0 60-61 0.0 0.0 0.0 0.3812105984700612 0.0 62-63 0.0 0.0 0.0 0.430964187608617 0.0 64-65 0.0 0.0 0.0 0.489254244025747 0.0 66-67 0.0 0.0 0.0 0.598954219053395 0.0 68-69 0.0 0.0 0.0 0.723752274267775 0.0 70-71 0.0 0.0 0.0 0.8264447015294292 0.0 72-73 0.0 0.0 0.0 0.9437892741199794 0.0 74-75 0.0 0.0 0.0 1.0401749083922391 0.0 76-77 0.0 0.0 0.0 1.1078933018036152 0.0 78-79 0.0 0.0 0.0 1.1980995530458625 0.0 80-81 0.0 0.0 0.0 1.3318800103966526 0.0 82-83 0.0 0.0 0.0 1.4861097662282066 0.0 84-85 0.0 0.0 0.0 1.6600243607842338 0.0 86-87 0.0 0.0 0.0 2.0142240478653735 0.0 88-89 0.0 0.0 0.0 2.481372663938395 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 70 0.008387838 27.144278 2 TAACGCA 90 8.9456025E-4 26.38859 4 GAGTACT 4925 0.0 22.184607 12-13 GTACATG 3925 0.0 22.157537 1 TACATGG 3905 0.0 21.774508 2 GTACTTT 5080 0.0 21.318655 14-15 ACATGGG 3780 0.0 21.23653 3 AGTACTT 5150 0.0 20.708054 12-13 AGAGTAC 7425 0.0 20.281815 10-11 ACTATCC 235 1.12933776E-7 20.215109 8 TACACGA 120 0.0047607366 19.792702 2 ACCGTGG 150 7.712053E-4 19.002205 8 AGTACAT 3040 0.0 18.75098 2 GCTACAC 330 5.966285E-10 18.711908 3 GTAAGGT 280 3.484456E-8 18.660501 4 GTGTAGC 510 0.0 18.636738 1 ACTTTTT 5885 0.0 18.44404 16-17 TACTTTT 5640 0.0 18.44394 14-15 GTATAGG 260 3.2596836E-7 18.27834 1 TAAGGTG 390 1.4551915E-11 18.269024 5 >>END_MODULE