FastQCFastQC Report
Thu 26 May 2016
SRR936919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164910
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54740.4699075465057386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.328780764179207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.22139049368620753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59000.033.7473831
GGTATCA37600.032.3541641
ATCAACG70850.027.6981623
CAACGCA71600.027.474395
TCAACGC71600.027.474394
AACGCAG73150.027.0221396
TATCAAC72650.027.0153872
GTACATG50200.024.4227221
ACGCAGA82450.023.7436397
ACATGGG48850.023.539193
GTATAGG4450.023.4931241
CGCAGAG82750.023.4863078
TACATGG51850.023.463242
GAGTACT41850.022.42155312-13
GCAGAGT86750.021.6912779
GTGTAGT5400.021.1200791
GTACTTT46400.020.32572214-15
AGAGTAC68800.019.99233610-11
AGTACTT46150.019.81769412-13
GAGTACG1200.004751843419.8000761
Produced by FastQC (version 0.11.5)